##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938941_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2770576 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.56290930117059 33.0 31.0 34.0 30.0 34.0 2 31.644678579472284 33.0 31.0 34.0 30.0 34.0 3 31.68692863866575 33.0 31.0 34.0 28.0 34.0 4 35.232431090141546 37.0 35.0 37.0 33.0 37.0 5 35.28144725140187 37.0 35.0 37.0 33.0 37.0 6 35.3601139257685 37.0 35.0 37.0 33.0 37.0 7 35.272323516842704 37.0 35.0 37.0 32.0 37.0 8 35.25741903488661 37.0 35.0 37.0 32.0 37.0 9 36.81791078822599 39.0 37.0 39.0 33.0 39.0 10-11 36.68453906335722 39.0 37.0 39.0 32.0 39.0 12-13 36.574053193271 39.0 37.0 39.0 32.0 39.0 14-15 37.70496099006127 40.0 37.0 41.0 32.0 41.0 16-17 37.62206414839369 40.0 37.0 41.0 32.0 41.0 18-19 37.55048950110013 40.0 37.0 41.0 32.0 41.0 20-21 37.66902117104891 40.0 37.5 41.0 32.0 41.0 22-23 37.59270924168837 40.0 37.0 41.0 32.0 41.0 24-25 37.499288234648674 40.0 37.0 41.0 32.0 41.0 26-27 37.371897937468596 40.0 37.0 41.0 31.0 41.0 28-29 37.239504348554235 40.0 37.0 41.0 31.0 41.0 30-31 37.03662649932721 40.0 36.5 41.0 30.5 41.0 32-33 36.81577332655736 39.5 36.0 41.0 30.0 41.0 34-35 36.4702971512061 39.0 35.5 41.0 30.0 41.0 36-37 36.18288706030803 39.0 35.0 41.0 29.5 41.0 38-39 35.93692539024376 39.0 35.0 40.5 28.0 41.0 40-41 35.79126741154186 39.0 35.0 40.0 27.5 41.0 42-43 35.56776334596127 39.0 35.0 40.0 27.0 41.0 44-45 35.465449603259394 39.0 35.0 41.0 26.0 41.0 46-47 35.22730399743591 39.0 35.0 41.0 24.5 41.0 48-49 34.9645912979828 39.0 35.0 41.0 24.0 41.0 50-51 33.87353983431604 38.0 33.0 39.5 22.5 40.5 52-53 34.00563312466433 38.0 33.5 39.5 23.0 40.5 54-55 34.50888118571734 38.0 34.0 40.0 23.0 41.0 56-57 34.41438946269656 38.0 34.0 40.0 22.5 41.0 58-59 34.20701056386831 38.0 34.0 40.0 22.0 41.0 60-61 33.878942681954946 38.0 33.5 40.0 20.5 41.0 62-63 33.51090242606592 37.5 33.0 40.0 19.5 41.0 64-65 33.125692996690944 37.0 32.5 40.0 18.0 41.0 66-67 32.69660803385289 36.0 32.0 39.0 14.5 41.0 68-69 32.24219891459393 36.0 31.5 39.0 12.0 40.5 70-71 31.773230909384907 35.0 31.0 38.5 8.0 40.0 72-73 31.320755503548718 35.0 31.0 38.0 7.0 40.0 74-75 30.840434083021 35.0 30.5 37.0 4.0 39.0 76-77 30.396646220858045 35.0 30.0 37.0 2.0 39.0 78-79 29.96838689860881 34.5 29.5 36.0 2.0 39.0 80-81 29.46754303076328 34.0 29.0 36.0 2.0 37.5 82-83 28.972203433509854 34.0 29.0 35.0 2.0 37.0 84-85 28.524703888288933 34.0 28.5 35.0 2.0 36.5 86-87 28.05769829089691 34.0 27.5 35.0 2.0 36.0 88-89 27.537161045212258 34.0 26.0 35.0 2.0 36.0 90-91 27.03402216723165 33.0 25.5 35.0 2.0 35.0 92-93 26.48897647998106 33.0 25.0 35.0 2.0 35.0 94-95 25.909721841234457 33.0 23.5 35.0 2.0 35.0 96-97 25.198827247474895 32.0 19.0 35.0 2.0 35.0 98-99 24.290238022707193 32.0 4.5 34.0 2.0 35.0 100-101 21.116490758600378 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.37228706961729685 1101 2 0.45116301192908637 1101 3 0.5192651996744146 1101 4 0.43753607685516727 1101 5 0.34538208149377425 1101 6 0.3807519567983917 1101 7 0.5044061827242885 1101 8 0.4876645338109924 1101 9 0.6330064532336479 1101 10-11 0.4509381236081609 1101 12-13 0.5114039013436411 1101 14-15 0.6215115218446314 1101 16-17 0.5014313570913842 1101 18-19 0.6741390557275224 1101 20-21 0.5507634442469467 1101 22-23 0.6112733539984134 1101 24-25 0.7496216740764083 1101 26-27 0.7591754469197056 1101 28-29 0.6751858269721964 1101 30-31 0.7193280187700424 1101 32-33 0.7367696360597549 1101 34-35 0.651524137514599 1101 36-37 0.6145895922345943 1101 38-39 0.8756470257424454 1101 40-41 0.7543322826377121 1101 42-43 0.6555578241515221 1101 44-45 0.7768642090799815 1101 46-47 0.8836295053898127 1101 48-49 0.8171465811880907 1101 50-51 0.7950291334805826 1101 52-53 0.8901478816386685 1101 54-55 0.9208510989057288 1101 56-57 0.979363123315018 1101 58-59 0.9035146214035237 1101 60-61 1.0296504719808084 1101 62-63 0.8501850933521595 1101 64-65 0.8205481448230998 1101 66-67 1.0136425594922684 1101 68-69 1.0148155233074547 1101 70-71 0.9053345123173067 1101 72-73 0.8416518623467546 1101 74-75 0.6181779580407749 1101 76-77 0.5659396385201312 1101 78-79 0.4482026664712997 1101 80-81 0.608846130368164 1101 82-83 0.7134474717681947 1101 84-85 0.7405911156848539 1101 86-87 0.6398295832305507 1101 88-89 0.4720754234404332 1101 90-91 0.413389098798433 1101 92-93 0.30893437829870507 1101 94-95 0.1751621381592514 1101 96-97 -0.1263947925718476 1101 98-99 0.001990768142530186 1101 100-101 0.3562844332202637 1102 1 0.23356437708585176 1102 2 0.2877357114817336 1102 3 0.3508735318375429 1102 4 0.18271150665315616 1102 5 0.3523656719369157 1102 6 0.39387032426814983 1102 7 0.4739861893442807 1102 8 0.4005332724015389 1102 9 0.5589114362824574 1102 10-11 0.5025176437590133 1102 12-13 0.5248786576075517 1102 14-15 0.6032742578072359 1102 16-17 0.7701243724979108 1102 18-19 0.7714338234259728 1102 20-21 0.7092677522571407 1102 22-23 0.7356900298595122 1102 24-25 0.7520800358288966 1102 26-27 0.8167073181708773 1102 28-29 0.783901021860764 1102 30-31 0.7653943265194414 1102 32-33 0.6779237966246612 1102 34-35 0.7391757202946039 1102 36-37 0.5242389207065798 1102 38-39 0.8055359151885781 1102 40-41 0.788585414596227 1102 42-43 0.6883180099125212 1102 44-45 0.7474635440716568 1102 46-47 0.7971850055001468 1102 48-49 0.8832985476267297 1102 50-51 0.7720553777382406 1102 52-53 0.7825426011917216 1102 54-55 0.9159190165169164 1102 56-57 0.7725109296902701 1102 58-59 0.7294809146089989 1102 60-61 0.7566215496353266 1102 62-63 0.6862540842045277 1102 64-65 0.6874762677543558 1102 66-67 0.6949595116879053 1102 68-69 0.8459756268200636 1102 70-71 0.6591206339044966 1102 72-73 0.6465156760641868 1102 74-75 0.5602082018771597 1102 76-77 0.7472691285801112 1102 78-79 0.7451506995311412 1102 80-81 0.5645128689185874 1102 82-83 0.6455176361646728 1102 84-85 0.7706515858046146 1102 86-87 0.7839112724319435 1102 88-89 0.49739062535020295 1102 90-91 0.5182351087484811 1102 92-93 0.3642273004235186 1102 94-95 0.40028544805513633 1102 96-97 0.5224352993056236 1102 98-99 0.33839671409931427 1102 100-101 0.4871530940760458 1103 1 0.37050747316677857 1103 2 0.24039069570245175 1103 3 0.43215740804218683 1103 4 0.3825086390756809 1103 5 0.2115531916532234 1103 6 0.4736364342854671 1103 7 0.27455969236984146 1103 8 0.2867273045785552 1103 9 0.5716592383425549 1103 10-11 0.5196228281665185 1103 12-13 0.5262402519197806 1103 14-15 0.5956885440737167 1103 16-17 0.6338530336841117 1103 18-19 0.6581075931594285 1103 20-21 0.6616103927276598 1103 22-23 0.640346164951616 1103 24-25 0.6903308921245852 1103 26-27 0.8427763318005361 1103 28-29 0.8049497799047103 1103 30-31 0.7643910547030686 1103 32-33 0.6712087177157855 1103 34-35 0.6371401555010436 1103 36-37 0.7034597072143285 1103 38-39 0.7738026318136164 1103 40-41 0.6852558987976209 1103 42-43 0.597472269071794 1103 44-45 0.7622990929051454 1103 46-47 0.9009979994989052 1103 48-49 0.8121755066330181 1103 50-51 0.6602730981650211 1103 52-53 0.7268257130581617 1103 54-55 0.7325426865622191 1103 56-57 0.669698402025098 1103 58-59 0.7427804616170448 1103 60-61 0.7743151061271547 1103 62-63 0.7624260590524372 1103 64-65 0.6372503328739683 1103 66-67 0.6045143314449817 1103 68-69 0.5253511824615131 1103 70-71 0.5486482030721156 1103 72-73 0.5255948480352934 1103 74-75 0.5648163632136161 1103 76-77 0.4944940385241168 1103 78-79 0.4253903438707347 1103 80-81 0.503374073580428 1103 82-83 0.6374609130433804 1103 84-85 0.5280402923024887 1103 86-87 0.602037434369393 1103 88-89 0.6103978098798954 1103 90-91 0.6864183466746177 1103 92-93 0.6907370242268591 1103 94-95 0.6389743649018733 1103 96-97 0.47499583227254405 1103 98-99 0.5582220322722371 1103 100-101 0.7673411798764427 1104 1 0.39125395352409953 1104 2 0.43262788058347823 1104 3 0.399841800029094 1104 4 0.3825538843582521 1104 5 0.3298017376550746 1104 6 0.30858118797349476 1104 7 0.3335401066283552 1104 8 0.3326969671610982 1104 9 0.30369314133120184 1104 10-11 0.44888653558925995 1104 12-13 0.41683154095787955 1104 14-15 0.5257419183241652 1104 16-17 0.623882749813994 1104 18-19 0.6245891134980965 1104 20-21 0.5533602191102815 1104 22-23 0.5726063854510102 1104 24-25 0.5211472415226268 1104 26-27 0.5605303377540949 1104 28-29 0.5993926419451583 1104 30-31 0.46748551318840015 1104 32-33 0.5111998387086558 1104 34-35 0.6675263103231117 1104 36-37 0.5905005321238903 1104 38-39 0.5638955698752497 1104 40-41 0.6085720530943632 1104 42-43 0.45980689224995075 1104 44-45 0.5820126599004993 1104 46-47 0.659074586097617 1104 48-49 0.59170693555496 1104 50-51 0.4389989181294922 1104 52-53 0.4945507043355377 1104 54-55 0.5118160048117701 1104 56-57 0.6009359728574921 1104 58-59 0.5240711425160356 1104 60-61 0.6842119347365028 1104 62-63 0.6435561495642617 1104 64-65 0.6650121853919657 1104 66-67 0.7139444504097057 1104 68-69 0.6926493346016613 1104 70-71 0.6806588305513053 1104 72-73 0.5651654915598563 1104 74-75 0.5636031671384778 1104 76-77 0.5631352315905964 1104 78-79 0.528885739328171 1104 80-81 0.6613375014237661 1104 82-83 0.8269325179846483 1104 84-85 0.8295070289728166 1104 86-87 0.795443481021838 1104 88-89 0.6792424548881435 1104 90-91 0.6526525362572109 1104 92-93 0.5574640837298723 1104 94-95 0.6519271175914625 1104 96-97 0.7218519683679254 1104 98-99 0.8690225822605306 1104 100-101 0.9767314131507909 1105 1 0.26711329950904883 1105 2 0.2053437247243508 1105 3 0.27025822714434256 1105 4 0.3550009456173697 1105 5 0.45825744736956153 1105 6 0.4087957687824044 1105 7 0.5077962039247907 1105 8 0.4624923692588041 1105 9 0.49380862246522383 1105 10-11 0.5174729038916936 1105 12-13 0.35723896059999305 1105 14-15 0.4656547786285543 1105 16-17 0.5254049501706604 1105 18-19 0.5796600646374657 1105 20-21 0.6108370551126825 1105 22-23 0.41319660424073845 1105 24-25 0.4753663626104583 1105 26-27 0.3993692894629959 1105 28-29 0.5196454946877509 1105 30-31 0.5561464482855314 1105 32-33 0.4589318903320887 1105 34-35 0.5181679344577219 1105 36-37 0.6531643448954014 1105 38-39 0.6386265140177088 1105 40-41 0.548188061326428 1105 42-43 0.5508836266908119 1105 44-45 0.6888780956477447 1105 46-47 0.718584982544364 1105 48-49 0.6771512695311586 1105 50-51 0.6612351328884998 1105 52-53 0.6468222050370542 1105 54-55 0.6810995751196245 1105 56-57 0.5660923967203217 1105 58-59 0.5786478109331412 1105 60-61 0.7270641837876752 1105 62-63 0.7144070522260435 1105 64-65 0.7208150610572446 1105 66-67 0.8481662823291245 1105 68-69 0.7685106896884193 1105 70-71 0.7738832657134402 1105 72-73 0.708486953236747 1105 74-75 0.7009134107478943 1105 76-77 0.7161091171978704 1105 78-79 0.7081412972244401 1105 80-81 0.7548146029770173 1105 82-83 0.7571529907030126 1105 84-85 0.810297540654652 1105 86-87 0.7557340932315562 1105 88-89 0.719467439350364 1105 90-91 0.8117148738821136 1105 92-93 0.7925445009416983 1105 94-95 0.9119141370467041 1105 96-97 0.6933336416469125 1105 98-99 0.7732658780843202 1105 100-101 0.7404378187351632 1106 1 0.17545103837170828 1106 2 0.11412416669827152 1106 3 0.18630351551837165 1106 4 0.12143095239043333 1106 5 0.13780714384378 1106 6 0.16700260382676646 1106 7 0.11540713562062166 1106 8 0.18068750920038923 1106 9 0.11651605278190402 1106 10-11 0.18706759986810084 1106 12-13 0.22602600736217937 1106 14-15 0.2469760588075971 1106 16-17 0.2365289701448816 1106 18-19 0.2591146157011792 1106 20-21 0.26763059167530656 1106 22-23 0.30936947300457973 1106 24-25 0.2476519138298272 1106 26-27 0.236583274786625 1106 28-29 0.12599078909163097 1106 30-31 0.06431310770702225 1106 32-33 0.08912628986428217 1106 34-35 0.17357986430393169 1106 36-37 0.1378521739954479 1106 38-39 0.3238432870948529 1106 40-41 0.38874728075545306 1106 42-43 0.3832300513408313 1106 44-45 0.36818197059709235 1106 46-47 0.4678412777769907 1106 48-49 0.40490259855197763 1106 50-51 0.30336491767896945 1106 52-53 0.31407984366398267 1106 54-55 0.3882924840170219 1106 56-57 0.2614616127978664 1106 58-59 0.4342111180389665 1106 60-61 0.7529079069250457 1106 62-63 0.6195552112289633 1106 64-65 0.38091001014708326 1106 66-67 0.437868772376369 1106 68-69 0.46570842246514843 1106 70-71 0.5217750621737842 1106 72-73 0.37825455163196864 1106 74-75 0.5457000452366714 1106 76-77 0.5603213734973131 1106 78-79 0.5750161105445208 1106 80-81 0.5731898671334932 1106 82-83 0.63228526175455 1106 84-85 0.69130064111895 1106 86-87 0.8204732882326695 1106 88-89 0.8187220518343956 1106 90-91 0.6768393481984596 1106 92-93 0.7884313725577989 1106 94-95 0.6583201366063811 1106 96-97 0.642300504120076 1106 98-99 0.7009355236834445 1106 100-101 0.8445163105436855 1107 1 0.15081557379654953 1107 2 0.1484601652178128 1107 3 0.18546177750490855 1107 4 0.22378716568515955 1107 5 0.15908790279592466 1107 6 0.14735198089164925 1107 7 0.030315124103537983 1107 8 0.15929136165539148 1107 9 0.2628772299632658 1107 10-11 0.2748685218882727 1107 12-13 0.3245354806567988 1107 14-15 0.420220737053036 1107 16-17 0.3218048141961134 1107 18-19 0.4418287785623747 1107 20-21 0.42180716887952485 1107 22-23 0.33678133952392386 1107 24-25 0.351396872382459 1107 26-27 0.3427286698564842 1107 28-29 0.3910515858172019 1107 30-31 0.4002067161586993 1107 32-33 0.3421400004239743 1107 34-35 0.4026726214925844 1107 36-37 0.35225424881298295 1107 38-39 0.5705528071980481 1107 40-41 0.5624581398017483 1107 42-43 0.591494078947008 1107 44-45 0.3570513256728347 1107 46-47 0.5236301656377904 1107 48-49 0.6125910439084024 1107 50-51 0.7281454344239293 1107 52-53 0.7032452902232791 1107 54-55 0.71912246545687 1107 56-57 0.5905789989196606 1107 58-59 0.7004819982035642 1107 60-61 0.689743810194571 1107 62-63 0.5721306356358227 1107 64-65 0.586669778716761 1107 66-67 0.40553737851482907 1107 68-69 0.6525339843041777 1107 70-71 0.4377778815743021 1107 72-73 0.4665458751717857 1107 74-75 0.5345757956707402 1107 76-77 0.6014604004231323 1107 78-79 0.5159777002945276 1107 80-81 0.6096714403372125 1107 82-83 0.5447798869321225 1107 84-85 0.649813679485657 1107 86-87 0.8659381627475753 1107 88-89 0.8980732087138925 1107 90-91 0.9768232917669089 1107 92-93 0.8189907078759582 1107 94-95 0.835201324455749 1107 96-97 0.9852432942380212 1107 98-99 0.8078037303161132 1107 100-101 0.8797333717268536 1108 1 0.31217955093497096 1108 2 0.23794622071358873 1108 3 0.30904615895457255 1108 4 0.3278489104680986 1108 5 0.27683892151207345 1108 6 0.4042669026577812 1108 7 0.33308164406331287 1108 8 0.3793880735395305 1108 9 0.4072934747373864 1108 10-11 0.41517958311708014 1108 12-13 0.4457679397758483 1108 14-15 0.49274169570463044 1108 16-17 0.4770347199097458 1108 18-19 0.4897249753554007 1108 20-21 0.28435683680451973 1108 22-23 0.41854516275241593 1108 24-25 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2316 38-39 -2.989631390805229 2316 40-41 -2.80770654019328 2316 42-43 -2.8347952803664143 2316 44-45 -2.8106822393807462 2316 46-47 -3.1415693859087597 2316 48-49 -3.4291908819129233 2316 50-51 -3.2095191353941885 2316 52-53 -3.0854640906625583 2316 54-55 -3.1322907149078603 2316 56-57 -2.9467354753895236 2316 58-59 -2.7932261206557456 2316 60-61 -2.8937970044557986 2316 62-63 -3.017154458606619 2316 64-65 -2.9587410854842915 2316 66-67 -3.2550237803594015 2316 68-69 -3.3003186408223044 2316 70-71 -3.1280910003236038 2316 72-73 -3.245071936006788 2316 74-75 -3.285613412600142 2316 76-77 -3.2716748830342617 2316 78-79 -3.220358581031885 2316 80-81 -3.0783511488248507 2316 82-83 -2.934113887827081 2316 84-85 -3.059239290906355 2316 86-87 -3.0701447005346623 2316 88-89 -3.1356342513096926 2316 90-91 -3.3185918570921267 2316 92-93 -3.2608934015851467 2316 94-95 -3.2668604529672898 2316 96-97 -3.536738559289443 2316 98-99 -3.6504058858364026 2316 100-101 -3.3433060578104445 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9870.0 3 3086.0 4 3136.0 5 3417.0 6 4016.0 7 4733.0 8 6050.0 9 7338.0 10 8992.0 11 11526.0 12 14778.0 13 19880.0 14 18027.0 15 18685.0 16 22779.0 17 29993.0 18 25464.0 19 19209.0 20 19723.0 21 20783.0 22 23176.0 23 26253.0 24 28910.0 25 33243.0 26 37983.0 27 44253.0 28 50924.0 29 60257.0 30 71205.0 31 85684.0 32 104544.0 33 131756.0 34 173732.0 35 241608.0 36 355978.0 37 516487.0 38 447485.0 39 65598.0 40 15.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.377229255772924 19.825222581584278 14.202167952597039 22.595380210045754 2 14.875576276586752 21.310872544705912 40.3325335362087 23.48101764249863 3 19.38269825287391 25.62981476684453 30.503732590247466 24.483754390034093 4 12.186964484494235 16.833834517737518 38.69516944464817 32.284031553120066 5 12.71445291313408 38.42683279800278 34.93188131018855 13.926832978674582 6 30.38682692425756 37.1157244996645 17.945087145662654 14.552361430415283 7 27.040252684830495 33.61829957063659 21.986628303604608 17.354819440928313 8 25.326350201050357 34.80944817508969 20.58398863399156 19.280212989868392 9 26.113231506214074 17.11290068605456 21.809391247567607 34.96447656016377 10-11 24.471218844333308 26.695518878443853 28.029947216234667 20.803315060988172 12-13 25.876640736065287 24.616273024837366 28.16821346817896 21.338872770918385 14-15 22.485097263905864 25.727125137480034 27.41473258678057 24.37304501183353 16-17 21.183017919069407 28.948176718662396 28.009400581983783 21.859404780284418 18-19 21.079698480157095 28.080525200151836 30.01640076669341 20.823375552997664 20-21 22.211800279622686 26.8238770758156 29.638090518725 21.32623212583671 22-23 21.558206241126094 26.973825257644723 29.70730938155694 21.760659119672248 24-25 21.877046223586838 27.07536858442015 29.78114789203183 21.266437299961183 26-27 21.69116963083445 26.805679859042105 30.276849327316192 21.226301182807255 28-29 21.0449638283419 27.723819867255894 30.008180233168254 21.223036071233956 30-31 21.42617578454117 27.06183225615356 29.830435618905028 21.681556340400245 32-33 21.30555073971336 27.237939927184467 29.917522682616738 21.538986650485437 34-35 22.071601853863424 27.05096313998117 29.645339995655007 21.232095010500398 36-37 21.853519499110153 26.695112522663315 29.990785051579955 21.46058292664658 38-39 21.435603765354 27.41540047046981 29.887995985014882 21.260999779161313 40-41 21.574757901072086 27.38282991983815 29.67603519725035 21.36637698183941 42-43 21.29273808824372 27.49331052856435 29.18477302944383 22.0291783537481 44-45 21.637952609110396 27.173122191311794 29.16064880470935 22.028276394868467 46-47 22.043771609167344 27.670777931837893 28.767715754377328 21.51773470461744 48-49 21.9537211373778 27.558466154552953 28.72660125013399 21.761211457935257 50-51 21.24149163264529 27.84478271357294 29.130110688123562 21.783614965658206 52-53 21.792533110084502 28.214213686275418 28.27983172184047 21.71342148179961 54-55 21.342578269370897 27.588767671111047 28.697245996083588 22.37140806343447 56-57 21.80111560054864 27.804000242955425 28.40803457219448 21.986849584301453 58-59 22.345541306303684 27.46298116151132 28.39523428057835 21.796243251606644 60-61 21.827542060757395 27.64141737662646 29.068615503655426 21.462425058960715 62-63 21.48293672215554 27.618180496090527 29.080423357370393 21.81845942438354 64-65 21.330815361466325 27.998368348426173 28.71937116482075 21.951445125286753 66-67 21.53443184374161 27.68253484541767 28.28330539895373 22.499727911886986 68-69 21.26262216704791 27.620578760635222 28.737278054768062 22.379521017548807 70-71 21.759717405513545 27.492734043967808 28.474583712103964 22.27296483841468 72-73 21.737697454282934 27.068130967162073 28.935522226162412 22.258649352392577 74-75 21.174920432337284 28.16403218767266 28.35766829834944 22.30337908164061 76-77 21.399083296686356 27.410897979353777 28.488309992610155 22.70170873134971 78-79 21.48401917553886 27.470083548490038 28.58435351454846 22.461543761422647 80-81 21.637031170217703 27.52918944088567 28.407610650791444 22.426168738105183 82-83 21.55326503104575 27.337162802109237 28.743392770068855 22.366179396776158 84-85 21.28900289147057 27.41692575657427 28.543048819520187 22.751022532434973 86-87 21.20613201157464 27.48557386556208 28.612608534889382 22.695685587973895 88-89 21.374104775720372 27.251940971190148 28.318294067177707 23.055660185911773 90-91 21.3961450416778 26.919339851673215 28.609250935045683 23.075264171603305 92-93 21.642500755931042 27.195487667576806 28.60051270898296 22.561498867509187 94-95 21.400960031809987 26.77843697882031 28.686074822402098 23.134528166967606 96-97 20.979771001020293 26.798086384763632 29.02401088311983 23.198131731096247 98-99 21.20019619146226 26.92693355429058 28.37087079188722 23.501999462359933 100-101 21.31437110545291 25.96104183441782 29.21378207220735 23.510804987921915 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2381.0 1 1410.0 2 541.0 3 662.5 4 636.5 5 673.5 6 1075.0 7 1963.0 8 3063.5 9 3705.0 10 3609.5 11 3458.5 12 3650.0 13 3942.0 14 4346.5 15 4719.5 16 5061.0 17 5228.5 18 5310.0 19 5661.5 20 6239.0 21 7138.5 22 8467.5 23 10117.5 24 12063.0 25 14819.5 26 18790.5 27 22959.5 28 26211.0 29 30080.5 30 35211.5 31 41192.5 32 47612.0 33 54196.0 34 61402.5 35 67608.5 36 74891.0 37 82413.0 38 85452.0 39 85930.0 40 86602.0 41 88693.0 42 90408.0 43 92546.0 44 95725.0 45 97787.0 46 99482.0 47 108542.0 48 131996.5 49 132026.5 50 107508.5 51 93631.5 52 85629.5 53 81577.5 54 77714.0 55 70153.5 56 62454.5 57 55628.0 58 49645.0 59 43940.0 60 37469.0 61 30753.0 62 24800.5 63 19214.0 64 14264.5 65 10699.5 66 7887.0 67 6220.0 68 4616.5 69 2904.0 70 2067.0 71 1516.5 72 1065.5 73 770.5 74 574.5 75 430.5 76 293.0 77 178.0 78 111.5 79 78.5 80 59.5 81 46.5 82 32.0 83 23.5 84 19.0 85 16.5 86 12.0 87 8.5 88 9.0 89 7.0 90 5.5 91 6.5 92 6.0 93 4.5 94 1.5 95 3.0 96 3.5 97 1.5 98 2.5 99 1.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3209440924919584 2 0.08568615334861776 3 0.09597282297977028 4 0.10102592385121362 5 0.11286461732145228 6 0.05536754811995773 7 0.05749706920149456 8 0.05839940864282373 9 0.061359082010383405 10-11 0.05910323340706049 12-13 0.05711808663613631 14-15 0.05549387564174381 16-17 0.05769558387858698 18-19 0.06507672050865958 20-21 0.06659265077009258 22-23 0.08220312310508718 24-25 0.09093776889715352 26-27 0.08566810655979118 28-29 0.08364686621121384 30-31 0.07790798736436033 32-33 0.06987716633653074 34-35 0.316757237484191 36-37 0.4924066331333268 38-39 0.4658598067694227 40-41 0.4477769243651861 42-43 0.4785286525256842 44-45 0.48747985978366953 46-47 0.48202972955804135 48-49 0.4992283193097753 50-51 0.49560091475563206 52-53 0.4949151367802219 54-55 0.4859278359445834 56-57 0.4644702040297758 58-59 0.4482641876635039 60-61 0.40870562655563314 62-63 0.4777526406061411 64-65 0.5002569862728906 66-67 0.5094969421521012 68-69 0.5220033668089235 70-71 0.5197655649944272 72-73 0.5083239008783733 74-75 0.5095330357297544 76-77 0.48414120385075166 78-79 0.46546277741523784 80-81 0.47181524708219513 82-83 0.4791241965569615 84-85 0.48107324975023247 86-87 0.48150637268207047 88-89 0.4878227487713746 90-91 0.5014300275466185 92-93 0.5068079706169403 94-95 0.5128536448738458 96-97 0.5057973504426516 98-99 0.5083780412448531 100-101 0.4878407955602012 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2770576.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.473823109629436 #Duplication Level Percentage of deduplicated Percentage of total 1 70.97663336314127 32.98555503835651 2 14.753529805943485 13.71305868888126 3 5.756353228878383 8.025592251463147 4 2.7569036344106728 5.124954073435844 5 1.5531538282968518 3.6090498139155827 6 0.9486647086188708 2.645284551522093 7 0.6458864027181054 2.1011767300185276 8 0.46811904095096674 1.7404225202723684 9 0.3193368560978978 1.3356724106411004 >10 1.6129242737426928 13.543853763822492 >50 0.12951745832151138 4.1536463334106095 >100 0.07343197441767513 6.128194735111282 >500 0.0031241827953018254 0.9244783374458307 >1k 0.0018745096771528675 1.8216241455949609 >5k 3.905228494068474E-4 1.2184572943973626 >10k+ 1.5620913976273896E-4 0.9289793117109063 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 14893 0.5375416519886118 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10700 0.3862012808888838 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8379 0.3024280871558838 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 7777 0.2806997534086775 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 6212 0.22421330438147155 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 5694 0.2055168311571312 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5506 0.19873123855833588 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 4181 0.15090724816789 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3663 0.13221077494354963 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3527 0.12730204838271897 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3418 0.12336784841852382 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2982 0.10763104856174313 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2960 0.10683698985337343 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2953 0.10658433480980128 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 7.218715530633342E-5 0.0 0.0 0.0 0.0 3 7.218715530633342E-5 0.0 0.0 0.0 0.0 4 7.218715530633342E-5 0.0 0.0 0.0 0.0 5 7.218715530633342E-5 0.0 0.0 0.0 0.0 6 7.218715530633342E-5 0.0 0.0 0.0 0.0 7 2.1656146591900023E-4 0.0 0.0 3.609357765316671E-5 0.0 8 2.5265504357216694E-4 0.0 0.0 3.609357765316671E-5 0.0 9 2.5265504357216694E-4 0.0 0.0 3.609357765316671E-5 0.0 10-11 2.887486212253337E-4 0.0 0.0 9.023394413291676E-5 0.0 12-13 3.428889877050837E-4 0.0 0.0 1.0828073295950011E-4 0.0 14-15 4.3312293183800046E-4 0.0 0.0 1.443743106126668E-4 0.0 16-17 4.5116972066458385E-4 0.0 0.0 1.9851467709241686E-4 0.0 18-19 5.414036647975007E-4 0.0 0.0 3.6093577653166704E-4 0.0 20-21 6.13590820103834E-4 0.0 0.0 5.955440312772507E-4 0.0 22-23 6.13590820103834E-4 0.0 0.0 8.481990748494176E-4 0.0 24-25 7.218715530633341E-4 0.0 0.0 0.0013174155843405848 0.0 26-27 7.760119195430842E-4 0.0 0.0 0.0019129596156178355 0.0 28-29 0.001173041273727918 0.0 0.0 0.004674118306085088 0.0 30-31 0.0012632752178608347 0.0 0.0 0.01351704483111093 0.0 32-33 0.0012632752178608347 0.0 0.0 0.030318605228660034 0.0 34-35 0.001335462373167168 0.0 0.0 0.05580067105179573 0.0 36-37 0.001479836683779835 0.0 0.0 0.09629766517864877 0.0 38-39 0.0016963981496988351 0.0 0.0 0.1585951802080145 0.0 40-41 0.0017505385161785854 0.0 0.0 0.23399466392547974 0.0 42-43 0.0019490531932710021 0.0 0.0 0.310910077904378 0.0 44-45 0.0020753807150570855 0.0 0.0 0.4071175091388939 0.0 46-47 0.0023821761251090028 0.0 0.0 0.5234471099150502 0.0 48-49 0.002490456858068503 0.0 0.0 0.648800105104498 0.0 50-51 0.002490456858068503 0.0 0.0 0.7898718533619002 0.0 52-53 0.002490456858068503 0.0 0.0 0.9291930631031238 0.0 54-55 0.0025265504357216695 0.0 0.0 1.0800642176933604 0.0 56-57 0.0026348311686811695 0.0 0.0 1.2722083783299936 0.0 58-59 0.0026348311686811695 0.0 0.0 1.483482135122805 0.0 60-61 0.002652877957507753 0.0 0.0 1.7184874192225732 0.0 62-63 0.002707018323987503 0.0 0.0 1.9556222244038786 0.0 64-65 0.0027431119016406696 0.0 0.0 2.2004810552029612 0.0 66-67 0.0030318605228660035 0.0 0.0 2.480603311369188 0.0 68-69 0.0030318605228660035 0.0 0.0 2.80802620104989 0.0 70-71 0.00317623483347867 0.0 0.0 3.1718675105826373 0.0 72-73 0.0032484219887850036 0.0 0.0 3.5335612522450206 0.0 74-75 0.0033025623552647536 0.0 0.0 3.910576717621173 0.0 76-77 0.003320609144091337 0.0 0.0 4.309717546098717 0.0 78-79 0.0033386559329179203 0.0 0.0 4.782489272988721 0.0 80-81 0.0037898256535825042 0.0 0.0 5.304023423288154 0.0 82-83 0.0037898256535825042 0.0 0.0 5.830105364371885 0.0 84-85 0.003916153175368588 0.0 0.0 6.35896651093491 0.0 86-87 0.004042480697154671 0.0 0.0 6.916576192098683 0.0 88-89 0.004096621063634421 0.0 0.0 7.525095864542247 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 12205 0.0 24.308193 1 TACATGG 12090 0.0 23.62344 2 ACATGGG 12150 0.0 22.454239 3 GTAAGCG 300 0.0 21.40389 94-95 GAGTACT 9775 0.0 20.923712 12-13 TTTGCGT 605 0.0 20.354954 3 GTTCGCG 165 6.490896E-5 20.084558 7 CATGGGG 4655 0.0 19.94291 4 AGTACTT 10395 0.0 19.584642 12-13 TCGCGCA 170 8.165154E-5 19.493837 9 CGTTCGC 175 1.0200414E-4 18.93687 6 GTACTTT 10840 0.0 18.868525 14-15 CATGGGA 7800 0.0 18.581423 4 AGAGTAC 17640 0.0 18.410679 10-11 TTCGCGC 185 1.5615989E-4 17.913254 8 GTGTAGC 1540 0.0 17.569708 1 CTTTGCG 730 0.0 16.869516 2 AGTACAT 9285 0.0 16.833878 2 CCTATAC 765 0.0 16.71685 3 ACTTTTT 13075 0.0 16.188559 16-17 >>END_MODULE