FastQCFastQC Report
Fri 27 May 2016
SRR938941_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938941_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2770576
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT161900.584355022204769No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108710.3923732826675752No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89160.32181033835563433No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT74030.2672007553663931No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58090.20966759258724538No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT58000.2093427503883669No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC54490.19667390463210538No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT43570.15725971783484732No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC37620.13578403913121315No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT37290.13459295106865865No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC36220.1307309382597698No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG33740.12177973100178446No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA32850.11856740259065263No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC29850.10773932929470263No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG118150.025.6848071
TACATGG118400.024.808332
ACATGGG120250.023.006053
GAGTACT105800.021.43978712-13
CATGGGG45800.021.1360234
GTAAGCG2350.020.19289494-95
AGTACTT111100.020.16072712-13
TTTGCGT6900.019.9437833
GTACTTT114850.019.64724314-15
CATGGGA74450.019.12124
CTAGACG1751.0019934E-418.9809783
TTGCGTT7750.018.3686894
AGAGTAC189450.018.18512310-11
CTTTGCG8050.017.6841412
TCGCGCT2550.016.74867860-61
GTCGGGA5150.016.5853212
ACTTTTT138100.016.4421516-17
AGTACAT92650.015.979672
TACTTTT137650.015.96198514-15
CGGGAGT5650.015.9574594
Produced by FastQC (version 0.11.5)