FastQCFastQC Report
Fri 27 May 2016
SRR938940_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938940_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2795698
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT153090.5475913349725186No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109050.39006359055949535No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83250.29777894464995863No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT78230.2798227848644596No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT63000.22534622838375246No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC56520.2021677591785665No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55920.20002160462253074No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT42080.1505169728633064No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT37810.13524350627285206No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC34130.12208042499583288No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC33750.12072119377701027No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG30980.11081311357664526No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC30220.10809465113899999No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA29930.10705734310358272No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG118550.025.6363751
TACATGG116950.025.2175672
ACATGGG118800.023.9083823
GAGTACT94500.021.6894312-13
CATGGGG43200.021.3681164
TTTGCGT6850.020.7322313
CATGGGA74250.019.8917734
GTACTTT105850.019.18536814-15
AGTACTT100700.019.17885812-13
GTATAGG8550.018.299871
CTTTGCG7650.017.945352
AGTACAT90850.017.8724692
AGAGTAC176950.017.85603310-11
TTGCGTT8150.017.4252514
GGACCGT2858.9160494E-716.6079526
GAGTACA114150.016.1990241
ACTTTTT128700.016.16661316-17
ATGGGAT32400.016.0714575
GTATCAA312550.015.4580561
GTGTAGC14950.015.2229991
Produced by FastQC (version 0.11.5)