##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938940_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2795698 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.575521748057195 33.0 31.0 34.0 30.0 34.0 2 31.642432766343145 33.0 31.0 34.0 30.0 34.0 3 31.684560349508423 33.0 31.0 34.0 28.0 34.0 4 35.233012650150336 37.0 35.0 37.0 33.0 37.0 5 35.271985386118246 37.0 35.0 37.0 33.0 37.0 6 35.35098605071077 37.0 35.0 37.0 33.0 37.0 7 35.25250080659642 37.0 35.0 37.0 32.0 37.0 8 35.2343325352023 37.0 35.0 37.0 32.0 37.0 9 36.79602160176099 39.0 37.0 39.0 32.0 39.0 10-11 36.67424253263407 39.0 37.0 39.0 32.0 39.0 12-13 36.546023211376905 39.0 37.0 39.0 32.0 39.0 14-15 37.686235959678044 40.0 37.0 41.0 32.0 41.0 16-17 37.61841085839744 40.0 37.0 41.0 32.0 41.0 18-19 37.57487039014944 40.0 37.0 41.0 32.0 41.0 20-21 37.45314998258038 40.0 37.0 41.0 32.0 41.0 22-23 37.5293184385438 40.0 37.0 41.0 32.0 41.0 24-25 37.44742726145671 40.0 37.0 41.0 32.0 41.0 26-27 37.35454759419652 40.0 37.0 41.0 31.0 41.0 28-29 37.22401078371126 40.0 37.0 41.0 31.0 41.0 30-31 37.033869180433655 40.0 36.5 41.0 30.5 41.0 32-33 36.82950340129728 40.0 36.0 41.0 30.0 41.0 34-35 36.48399165432032 39.0 35.5 41.0 30.0 41.0 36-37 36.2016085070705 39.0 35.0 41.0 29.5 41.0 38-39 35.987683040156696 39.0 35.0 41.0 28.5 41.0 40-41 35.845361516158036 39.0 35.0 41.0 27.5 41.0 42-43 35.62568685888104 39.0 35.0 40.5 27.0 41.0 44-45 35.52135477437119 39.0 35.0 41.0 26.0 41.0 46-47 35.26846318879936 39.0 35.0 41.0 24.5 41.0 48-49 35.06560758708559 39.0 35.0 41.0 24.0 41.0 50-51 33.91399088885852 38.0 33.5 40.0 23.0 40.5 52-53 34.09007893556458 38.0 33.5 39.5 23.0 40.5 54-55 34.597634114986676 39.0 34.0 40.0 23.0 41.0 56-57 34.495955929431574 38.5 34.0 40.0 23.0 41.0 58-59 34.29519282840994 38.0 34.0 40.0 22.0 41.0 60-61 34.01310835433584 38.0 34.0 40.0 22.0 41.0 62-63 33.67270052058556 37.5 33.5 40.0 20.5 41.0 64-65 33.269962277756754 37.0 33.0 40.0 18.5 41.0 66-67 32.87154263443333 36.5 32.0 39.0 17.5 41.0 68-69 32.46573181366514 36.0 32.0 39.0 13.5 41.0 70-71 32.02687414735068 35.5 31.5 39.0 12.0 40.0 72-73 31.583376852578496 35.0 31.0 38.0 8.0 40.0 74-75 31.101977931808086 35.0 31.0 37.0 7.0 39.5 76-77 30.67854306867194 35.0 30.5 37.0 3.5 39.0 78-79 30.224535876192636 34.5 30.0 36.0 2.0 39.0 80-81 29.75096129839489 34.0 29.5 36.0 2.0 37.5 82-83 29.34038386835774 34.0 29.0 35.0 2.0 37.0 84-85 28.957295280105363 34.0 29.0 35.0 2.0 37.0 86-87 28.552092715307587 34.0 29.0 35.0 2.0 36.0 88-89 28.093465030915354 34.0 27.5 35.0 2.0 36.0 90-91 27.623222536912067 34.0 26.5 35.0 2.0 35.5 92-93 27.159551568159365 33.5 26.0 35.0 2.0 35.0 94-95 26.642576379852187 33.0 25.0 35.0 2.0 35.0 96-97 26.009075372232623 33.0 24.5 35.0 2.0 35.0 98-99 25.247370424130217 32.5 19.0 35.0 2.0 35.0 100-101 22.61289237964902 29.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.30297798550301636 1101 2 0.3865279119337224 1101 3 0.3408000796537003 1101 4 0.23586326261239066 1101 5 0.06840858582124554 1101 6 0.24420636650467742 1101 7 0.26877139666158456 1101 8 0.3581295130720292 1101 9 0.3431689596025933 1101 10-11 0.4505563120127718 1101 12-13 0.5430505287535965 1101 14-15 0.16129398185048416 1101 16-17 0.47175959714326154 1101 18-19 0.4623840723198853 1101 20-21 0.6144591722640271 1101 22-23 0.5152211385690109 1101 24-25 0.6371216787179463 1101 26-27 0.5867881331487368 1101 28-29 0.6297954375837165 1101 30-31 0.6532570155178021 1101 32-33 0.6141221882959869 1101 34-35 0.5431029182195672 1101 36-37 0.41744144701967656 1101 38-39 0.5492205575798721 1101 40-41 0.5302708205112623 1101 42-43 0.4440121794112386 1101 44-45 0.647543001984836 1101 46-47 0.539094208566361 1101 48-49 0.5144030400629731 1101 50-51 0.37371085324670617 1101 52-53 0.2774787345476284 1101 54-55 0.3647347583331637 1101 56-57 0.4640464991975577 1101 58-59 0.3399120398377775 1101 60-61 0.21350073512017786 1101 62-63 0.3196525218282815 1101 64-65 0.3080329629680776 1101 66-67 0.1545820163233529 1101 68-69 0.21242285770693314 1101 70-71 0.2622778640982091 1101 72-73 0.03983065843909728 1101 74-75 0.11817730562865947 1101 76-77 0.08561623946058461 1101 78-79 0.0018847712417731088 1101 80-81 -0.15667864665769216 1101 82-83 -0.16689327461747183 1101 84-85 -0.23065626661983885 1101 86-87 -0.14912464666726777 1101 88-89 -0.27669550600375103 1101 90-91 -0.2835833825930081 1101 92-93 -0.25862618781960833 1101 94-95 -0.27030537656352394 1101 96-97 -0.29945099301599853 1101 98-99 -0.446055951989873 1101 100-101 -0.010182601421430348 1102 1 0.3242873120776757 1102 2 0.2339628746011826 1102 3 0.30547087877086554 1102 4 0.25866333645313944 1102 5 0.3472610471216129 1102 6 0.2956197319130709 1102 7 0.38750494648609646 1102 8 0.299495775975295 1102 9 0.3686924916983614 1102 10-11 0.3422941297398907 1102 12-13 0.48854508183708134 1102 14-15 0.6721852025797688 1102 16-17 0.5915710071649443 1102 18-19 0.6866485092899595 1102 20-21 0.6827174593211183 1102 22-23 0.6474358502940163 1102 24-25 0.708870566752843 1102 26-27 0.7558756193471368 1102 28-29 0.7464969154436929 1102 30-31 0.7993643249395816 1102 32-33 0.7311332226121934 1102 34-35 0.6557325604053901 1102 36-37 0.7203729887472505 1102 38-39 0.6025514895407227 1102 40-41 0.5582088349148506 1102 42-43 0.5786905715869111 1102 44-45 0.732204733550482 1102 46-47 0.7077319453705186 1102 48-49 0.8125529286632869 1102 50-51 0.782295769531494 1102 52-53 0.7049351296095807 1102 54-55 0.7459888726911217 1102 56-57 0.6185917980610398 1102 58-59 0.7002927899251361 1102 60-61 0.6750187895324586 1102 62-63 0.6185958211863607 1102 64-65 0.7499057200261987 1102 66-67 0.7324903957394326 1102 68-69 0.7164029729495951 1102 70-71 0.7114050977519426 1102 72-73 0.6832478888768101 1102 74-75 0.7630868797323878 1102 76-77 0.6760531654096873 1102 78-79 0.5528797347451722 1102 80-81 0.46235139816921844 1102 82-83 0.4546605333478091 1102 84-85 0.36926371020623705 1102 86-87 0.35578820150755064 1102 88-89 0.41644276080517884 1102 90-91 0.4306395537843599 1102 92-93 0.4497828051945447 1102 94-95 0.49905068215148773 1102 96-97 0.4144754483589743 1102 98-99 0.7032550418068055 1102 100-101 0.779297529494972 1103 1 0.17483065273368936 1103 2 0.32323656933471057 1103 3 0.3993989139424947 1103 4 0.3520024846893577 1103 5 0.34882102852758123 1103 6 0.2804039745504241 1103 7 0.36252717910155496 1103 8 0.3578806440276381 1103 9 0.5040381967780974 1103 10-11 0.48724900450041986 1103 12-13 0.5816903525629229 1103 14-15 0.4773128431117968 1103 16-17 0.5579410824912614 1103 18-19 0.5825827286278695 1103 20-21 0.5868273211574291 1103 22-23 0.6776602588893752 1103 24-25 0.7020208444400566 1103 26-27 0.5656096643517543 1103 28-29 0.5548496365280542 1103 30-31 0.5494266985920362 1103 32-33 0.5407656348353314 1103 34-35 0.5760691732984284 1103 36-37 0.6986300455756975 1103 38-39 0.7676948024308601 1103 40-41 0.6857854286221254 1103 42-43 0.5224907016541067 1103 44-45 0.6447891620143764 1103 46-47 0.5972467281395168 1103 48-49 0.47142701382600904 1103 50-51 0.3314533198301177 1103 52-53 0.3376856071200791 1103 54-55 0.5871032658274871 1103 56-57 0.6575259724500881 1103 58-59 0.6337146412149721 1103 60-61 0.579825927638673 1103 62-63 0.47429121131114726 1103 64-65 0.3915479635317993 1103 66-67 0.4719274141394507 1103 68-69 0.4901617870507877 1103 70-71 0.3475740354738761 1103 72-73 0.4265101831006568 1103 74-75 0.2870174525230773 1103 76-77 0.3277126032758595 1103 78-79 0.4142037602510875 1103 80-81 0.36469282115902146 1103 82-83 0.42292571510904153 1103 84-85 0.43435072322946056 1103 86-87 0.3816532821054075 1103 88-89 0.4153139186024113 1103 90-91 0.4224816205669839 1103 92-93 0.245641521830688 1103 94-95 0.23399288588588618 1103 96-97 0.3329540802978137 1103 98-99 0.41885764383360424 1103 100-101 0.8557834164070606 1104 1 0.19057868422975233 1104 2 0.15600747297175843 1104 3 0.2456681302904471 1104 4 0.3343796875389984 1104 5 0.2749552617484241 1104 6 0.2616563180074891 1104 7 0.2856617872755365 1104 8 0.31851056528747534 1104 9 0.4312972893915159 1104 10-11 0.3089587907771332 1104 12-13 0.42327265477513976 1104 14-15 0.5016847966176172 1104 16-17 0.2776636171744258 1104 18-19 0.4513491328273389 1104 20-21 0.4059124355181325 1104 22-23 0.47968500579601425 1104 24-25 0.37337442524246 1104 26-27 0.3207652699010666 1104 28-29 0.4950446121561001 1104 30-31 0.5338661436614061 1104 32-33 0.498958360744588 1104 34-35 0.4069653112811764 1104 36-37 0.5229645037684207 1104 38-39 0.4994158373015054 1104 40-41 0.4851855036127475 1104 42-43 0.5502372333376471 1104 44-45 0.6222919741628559 1104 46-47 0.7086078080045368 1104 48-49 0.7104596347483962 1104 50-51 0.6066689178803628 1104 52-53 0.5350984305171522 1104 54-55 0.5336724446801355 1104 56-57 0.5367910643136042 1104 58-59 0.5538496243420852 1104 60-61 0.5355814581973561 1104 62-63 0.4538562656793488 1104 64-65 0.471225503839257 1104 66-67 0.554042149797489 1104 68-69 0.4779758102978775 1104 70-71 0.5671090935916183 1104 72-73 0.35189451005978967 1104 74-75 0.22158813118824483 1104 76-77 0.24597282074867266 1104 78-79 0.3879570410909814 1104 80-81 0.4526193303453745 1104 82-83 0.24782260299805614 1104 84-85 0.3115535728732546 1104 86-87 0.39383925885831417 1104 88-89 0.33676123768752575 1104 90-91 0.5055337390521757 1104 92-93 0.4891735803233743 1104 94-95 0.4321556155446764 1104 96-97 0.4454400195554058 1104 98-99 0.5525284349847084 1104 100-101 1.16455731966915 1105 1 0.09375832821072194 1105 2 0.15988626989009802 1105 3 0.22102935930809053 1105 4 0.052056138203660396 1105 5 0.04502944446453938 1105 6 0.12271111709682714 1105 7 0.1836239895140679 1105 8 0.1978179170618759 1105 9 0.23558957053593588 1105 10-11 0.19548699408873915 1105 12-13 0.25565203276725157 1105 14-15 0.1749744613763724 1105 16-17 0.35754748528207614 1105 18-19 0.3537496614905393 1105 20-21 0.22723856809410137 1105 22-23 0.2313908353849925 1105 24-25 0.2503166721024854 1105 26-27 0.1865985462663744 1105 28-29 0.17500947842627568 1105 30-31 0.24382332442817756 1105 32-33 0.1191791066320178 1105 34-35 0.2391696420934224 1105 36-37 0.30354193158994036 1105 38-39 0.3311327290436239 1105 40-41 0.319499101153383 1105 42-43 0.15056226176986343 1105 44-45 -0.02611343924886711 1105 46-47 0.11058988068743503 1105 48-49 0.11940444206184253 1105 50-51 -0.012878569622927216 1105 52-53 -0.03546650577188615 1105 54-55 -0.09038711693543888 1105 56-57 -0.0027453610365384407 1105 58-59 0.01161519370116082 1105 60-61 0.1110852108360092 1105 62-63 0.10572264149839583 1105 64-65 0.14346633561373778 1105 66-67 0.052079342662189276 1105 68-69 -0.047720287082213986 1105 70-71 -0.25344558845434406 1105 72-73 -0.02994450039539842 1105 74-75 -0.0066570260124088065 1105 76-77 -0.01914576594732509 1105 78-79 -0.002975514991444328 1105 80-81 -0.02340252051442704 1105 82-83 -0.039503359709645025 1105 84-85 -0.053068874182741865 1105 86-87 -0.07433012052175059 1105 88-89 -0.050103180503295874 1105 90-91 -0.032288259717315526 1105 92-93 -0.007178395016723016 1105 94-95 -0.12382488689251758 1105 96-97 -0.059765407114330316 1105 98-99 -0.03303131203586318 1105 100-101 0.29403692201167786 1106 1 0.12011675218473172 1106 2 0.23693731809555274 1106 3 0.31456525057587825 1106 4 0.2513114917480479 1106 5 0.2784944770572224 1106 6 0.21325879758344968 1106 7 0.19138170625695494 1106 8 0.32335638944536527 1106 9 0.30621046849035594 1106 10-11 0.27101255411560743 1106 12-13 0.2789613782632827 1106 14-15 0.3064332323233643 1106 16-17 0.372247372968296 1106 18-19 0.3016885985833042 1106 20-21 0.36232899864833 1106 22-23 0.3325639958486448 1106 24-25 0.43103537183834106 1106 26-27 0.3436908869004398 1106 28-29 0.3771083972451734 1106 30-31 0.12451213256682792 1106 32-33 0.22333241189028996 1106 34-35 0.3153257724804348 1106 36-37 0.2585903919015564 1106 38-39 0.18803968754988887 1106 40-41 0.24645000234098546 1106 42-43 0.47254065170405823 1106 44-45 0.35973956594115464 1106 46-47 0.22858285989007499 1106 48-49 0.2075401892263784 1106 50-51 0.1947212164589729 1106 52-53 0.11570824755465736 1106 54-55 0.13203395331930068 1106 56-57 0.23754810531101356 1106 58-59 0.42024390142844226 1106 60-61 0.43409570814135634 1106 62-63 0.30678735172274685 1106 64-65 0.13464634877689008 1106 66-67 0.3319877157662603 1106 68-69 0.30193724739649497 1106 70-71 0.34548522774893087 1106 72-73 0.21158468864760138 1106 74-75 0.2149686972998488 1106 76-77 0.09974192772954993 1106 78-79 0.190587895524569 1106 80-81 0.27155284882302766 1106 82-83 0.21196427704441234 1106 84-85 0.2481986379891019 1106 86-87 0.26423864811389564 1106 88-89 0.41593982950598374 1106 90-91 0.33839675944109615 1106 92-93 0.3599656560470734 1106 94-95 0.5163296953673289 1106 96-97 0.5165767154330858 1106 98-99 0.4724559311658325 1106 100-101 0.7163903464296553 1107 1 0.15366285646995692 1107 2 0.008464498876008975 1107 3 0.12022356717852745 1107 4 0.263558621399703 1107 5 0.16684300863204982 1107 6 0.1447314480566675 1107 7 0.03331250963559995 1107 8 0.19273517710269772 1107 9 0.1697473116585897 1107 10-11 0.21446931586041984 1107 12-13 0.23132568695478284 1107 14-15 0.22850179647819147 1107 16-17 0.3327600694680726 1107 18-19 0.3296982290820054 1107 20-21 0.3246642882874369 1107 22-23 0.2661040544979585 1107 24-25 0.2792334401580092 1107 26-27 0.36459383916383814 1107 28-29 0.3072571198886678 1107 30-31 0.2458507692294134 1107 32-33 0.4412276935039827 1107 34-35 0.3299442529928953 1107 36-37 0.22568392918320512 1107 38-39 0.3242539103402109 1107 40-41 0.25666659555206905 1107 42-43 0.40170679242254437 1107 44-45 0.3929197431646543 1107 46-47 0.3959293837822955 1107 48-49 0.37744873403516976 1107 50-51 0.38128641831013965 1107 52-53 0.35474996775730716 1107 54-55 0.4354238277428166 1107 56-57 0.5593943760837519 1107 58-59 0.6232453271355496 1107 60-61 0.42842702759143236 1107 62-63 0.3739726365120646 1107 64-65 0.4871459867030907 1107 66-67 0.40074499876447334 1107 68-69 0.40030745025082837 1107 70-71 0.43811154499999105 1107 72-73 0.3910689228249744 1107 74-75 0.42622022365683065 1107 76-77 0.5007969244144128 1107 78-79 0.4736757937559197 1107 80-81 0.32202268109981347 1107 82-83 0.4633966941427978 1107 84-85 0.5257056170446255 1107 86-87 0.6108727569273711 1107 88-89 0.7065100326260669 1107 90-91 0.7202055738453907 1107 92-93 0.6531413289396397 1107 94-95 0.7034314307970142 1107 96-97 0.4811524778807872 1107 98-99 0.6722911045427118 1107 100-101 0.877048470369175 1108 1 0.2228572162875615 1108 2 0.22856852925965399 1108 3 0.14846032890211802 1108 4 0.3351772701423812 1108 5 0.20123005086992407 1108 6 0.23048918055412315 1108 7 0.2626067724869401 1108 8 0.361839978718713 1108 9 0.4166591411461269 1108 10-11 0.4072571026087175 1108 12-13 0.5142890580471899 1108 14-15 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28.72597816624603 21.77525046139875 50-51 21.26354231555945 27.787778309234024 29.142985733967553 21.805693641238975 52-53 21.80308635268264 28.167645393245166 28.26383307273134 21.76543518134085 54-55 21.3384484519829 27.598005348354437 28.675320853599818 22.38822534606285 56-57 21.8620294408596 27.75410359921243 28.421422317314555 21.962444642613413 58-59 22.334135973949635 27.44187568678639 28.41083451547653 21.81315382378745 60-61 21.82010812132017 27.62961618369122 29.108142886925513 21.442132808063093 62-63 21.476877864188026 27.598632383939687 29.07633758030518 21.84815217156711 64-65 21.352921227124995 27.978435445319928 28.73560571372541 21.93303761382967 66-67 21.561288121539803 27.66838183006423 28.228131126868828 22.542198921527138 68-69 21.299794958627345 27.646324775097774 28.65019078114555 22.403689485129327 70-71 21.784598055639023 27.50054429871833 28.481235639060674 22.233622006581967 72-73 21.705261082198135 27.06216811699338 28.948060635567312 22.28451016524117 74-75 21.150943307966635 28.177473091137678 28.356235807021186 22.315347793874498 76-77 21.422838229209766 27.42177690633376 28.451188141965428 22.70419672249105 78-79 21.501389576048467 27.5170587927441 28.538275343059738 22.443276288147697 80-81 21.65481901580334 27.581216114243865 28.383827920604194 22.380136949348596 82-83 21.53824930511565 27.37011589019838 28.742995849938797 22.348638954747173 84-85 21.290151175566127 27.416679317993932 28.540063215864258 22.753106290575683 86-87 21.213219450533717 27.485778439344244 28.64026627970304 22.660735830418997 88-89 21.321709936011594 27.31559531236171 28.320968093580078 23.041726658046617 90-91 21.332482487741057 26.953298723803577 28.616297663065144 23.09792112539022 92-93 21.641215028736998 27.215782246537955 28.59194471672503 22.55105800800001 94-95 21.41813094342352 26.840066109707138 28.65272144790766 23.089081498961683 96-97 20.99935249042904 26.76355501645401 29.084478319404113 23.152614173712838 98-99 21.234523120680077 26.955498844181903 28.36166023266057 23.448317802477455 100-101 21.2208650778832 26.150403779521668 29.087091682972904 23.54163945962223 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3224.0 1 1892.0 2 622.5 3 712.5 4 649.0 5 662.5 6 1044.5 7 1951.0 8 3143.5 9 3764.5 10 3613.5 11 3466.0 12 3757.0 13 4208.5 14 4567.5 15 4872.5 16 5176.0 17 5419.0 18 5579.5 19 5831.0 20 6437.5 21 7307.0 22 8442.0 23 10188.0 24 12242.0 25 15000.5 26 19015.0 27 23279.5 28 26349.0 29 29890.5 30 35438.0 31 41494.0 32 47659.0 33 54576.0 34 61812.0 35 67680.0 36 75283.0 37 83097.0 38 85938.0 39 86583.0 40 87659.0 41 89664.0 42 91035.5 43 92895.5 44 96459.0 45 99058.0 46 100878.5 47 110007.5 48 133945.5 49 133656.0 50 108228.0 51 94324.0 52 86643.5 53 82479.5 54 78178.0 55 70636.5 56 62991.5 57 56344.5 58 50316.5 59 43844.0 60 37354.0 61 30957.0 62 24809.0 63 19312.5 64 14367.5 65 10797.0 66 7930.5 67 6110.5 68 4521.0 69 2930.5 70 2052.0 71 1432.5 72 1002.5 73 729.0 74 544.5 75 429.0 76 296.0 77 172.0 78 109.5 79 76.0 80 51.5 81 42.5 82 39.0 83 29.5 84 21.0 85 17.5 86 11.5 87 11.5 88 10.0 89 7.5 90 6.0 91 4.0 92 4.0 93 2.5 94 2.0 95 2.5 96 1.0 97 1.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2828989397281108 2 0.08702656724724916 3 0.09128310711672005 4 0.10591272734036365 5 0.10809465113899999 6 0.08323502753158603 7 0.09292849227634745 8 0.08902964483288252 9 0.0941446465247677 10-11 0.08790291369096376 12-13 0.09214156893913435 14-15 0.10092291799758057 16-17 0.08933368339498758 18-19 0.10024330238816925 20-21 0.10215695686730111 22-23 0.11480138412661167 24-25 0.12733850365812044 26-27 0.13000331223186481 28-29 0.122026771131932 30-31 0.11492657647571376 32-33 0.10952542084302383 34-35 0.37926127929411546 36-37 0.5804632689224659 38-39 0.5642776866456964 40-41 0.528222290104296 42-43 0.5536184523507188 44-45 0.574883267076773 46-47 0.5639915327048917 48-49 0.5858107706912549 50-51 0.5767253830707036 52-53 0.5807494228632706 54-55 0.5710380734972089 56-57 0.5452126803395789 58-59 0.5395611400086848 60-61 0.48782093058692316 62-63 0.5607901854921383 64-65 0.5937873117911877 66-67 0.6064317390504983 68-69 0.6077373164054201 70-71 0.6042498152518619 72-73 0.5962375049093286 74-75 0.5935905809568846 76-77 0.5776017295144182 78-79 0.5556215299363523 80-81 0.5508463360491727 82-83 0.5629721092907747 84-85 0.5728801894911396 86-87 0.5638663403557895 88-89 0.5770294216328087 90-91 0.5902640413950291 92-93 0.5974178899151482 94-95 0.5966667358205358 96-97 0.5933580808799805 98-99 0.5978471208263554 100-101 0.5754198057157819 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2795698.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.63699531435853 #Duplication Level Percentage of deduplicated Percentage of total 1 71.48984852202284 33.340717305457794 2 14.346443552426273 13.381500414644263 3 5.642526494121228 7.894514450024266 4 2.742746130203746 5.116537536911483 5 1.5598215814049283 3.637269589160848 6 0.9814034128963084 2.746182382124436 7 0.6368290652404435 2.078985589216612 8 0.4601254717043054 1.7167095574312559 9 0.3311014184828573 1.389741777212623 >10 1.6034033679622675 13.53359483974503 >50 0.12762632675075267 4.13535756311139 >100 0.07256766937610225 6.1047182926599435 >500 0.00331875636871008 1.0271422056692423 >1k 0.0016979683746625885 1.740735359117412 >5k 3.859019033324065E-4 1.212730417472423 >10k+ 1.543607613329626E-4 0.9435627200410215 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 15309 0.5475913349725186 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10905 0.39006359055949535 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8325 0.29777894464995863 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 7823 0.2798227848644596 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 6300 0.22534622838375246 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 5652 0.2021677591785665 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5592 0.20002160462253074 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 4208 0.1505169728633064 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3781 0.13524350627285206 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3413 0.12208042499583288 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3375 0.12072119377701027 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 3098 0.11081311357664526 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 3022 0.10809465113899999 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2993 0.10705734310358272 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 3.576924260059563E-5 0.0 0.0 0.0 0.0 3 3.576924260059563E-5 0.0 0.0 0.0 0.0 4 3.576924260059563E-5 0.0 0.0 0.0 0.0 5 7.153848520119126E-5 0.0 0.0 0.0 0.0 6 1.430769704023825E-4 0.0 0.0 0.0 0.0 7 2.1461545560357378E-4 0.0 0.0 3.576924260059563E-5 0.0 8 2.1461545560357378E-4 0.0 0.0 3.576924260059563E-5 0.0 9 2.1461545560357378E-4 0.0 0.0 7.153848520119126E-5 0.0 10-11 2.1461545560357378E-4 0.0 0.0 7.153848520119126E-5 0.0 12-13 3.2192318340536067E-4 0.0 0.0 1.9673083430327598E-4 0.0 14-15 3.934616686065519E-4 0.0 0.0 2.682693195044672E-4 0.0 16-17 4.2923091120714756E-4 0.0 0.0 3.2192318340536067E-4 0.0 18-19 6.080771242101257E-4 0.0 0.0 4.650001538077432E-4 0.0 20-21 6.796156094113169E-4 0.0 0.0 5.365386390089344E-4 0.0 22-23 7.153848520119126E-4 0.0 0.0 0.0010373080354172733 0.0 24-25 9.300003076154864E-4 0.0 0.0 0.0015201928105253143 0.0 26-27 9.300003076154864E-4 0.0 0.0 0.0021819237986363334 0.0 28-29 0.0011267311419187623 0.0 0.0 0.005329617147488749 0.0 30-31 0.0011803850058196557 0.0 0.0 0.015112504998751653 0.0 32-33 0.0011803850058196557 0.0 0.0 0.032317510689638146 0.0 34-35 0.0013055773549217405 0.0 0.0 0.05875098097147832 0.0 36-37 0.001538077431825612 0.0 0.0 0.10051157170767372 0.0 38-39 0.0017526928874291858 0.0 0.0 0.17049409485573908 0.0 40-41 0.0017526928874291858 0.0 0.0 0.24975873645865898 0.0 42-43 0.001985192964333057 0.0 0.0 0.3331904948245483 0.0 44-45 0.00228923152643812 0.0 0.0 0.43014302689346273 0.0 46-47 0.002593270088543183 0.0 0.0 0.5481636428541281 0.0 48-49 0.002682693195044672 0.0 0.0 0.6761102236364587 0.0 50-51 0.002682693195044672 0.0 0.0 0.8221023873107896 0.0 52-53 0.002682693195044672 0.0 0.0 0.9652151269557727 0.0 54-55 0.0027184624376452677 0.0 0.0 1.1217413325759793 0.0 56-57 0.002790000922846459 0.0 0.0 1.314072550039382 0.0 58-59 0.002790000922846459 0.0 0.0 1.527632812986238 0.0 60-61 0.002790000922846459 0.0 0.0 1.766106353404409 0.0 62-63 0.002790000922846459 0.0 0.0 2.00635047133131 0.0 64-65 0.0028436547867473523 0.0 0.0 2.2542849764173383 0.0 66-67 0.003004616378450033 0.0 0.0 2.54142257139362 0.0 68-69 0.0030403856210506285 0.0 0.0 2.876276335999096 0.0 70-71 0.003219231834053607 0.0 0.0 3.240568187264862 0.0 72-73 0.003380193425756287 0.0 0.0 3.604323499891619 0.0 74-75 0.0034159626683568827 0.0 0.0 3.9896476658065354 0.0 76-77 0.0034338472896571805 0.0 0.0 4.39346453014596 0.0 78-79 0.0034517319109574783 0.0 0.0 4.87209276538453 0.0 80-81 0.003648462745260754 0.0 0.0 5.410509289629996 0.0 82-83 0.0036842319878613496 0.0 0.0 5.9465650438638225 0.0 84-85 0.003737885851762243 0.0 0.0 6.485035221973189 0.0 86-87 0.0038094243369634346 0.0 0.0 7.047971562021363 0.0 88-89 0.0038273089582637324 0.0 0.0 7.658641956320031 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 11855 0.0 25.636375 1 TACATGG 11695 0.0 25.217567 2 ACATGGG 11880 0.0 23.908382 3 GAGTACT 9450 0.0 21.68943 12-13 CATGGGG 4320 0.0 21.368116 4 TTTGCGT 685 0.0 20.732231 3 CATGGGA 7425 0.0 19.891773 4 GTACTTT 10585 0.0 19.185368 14-15 AGTACTT 10070 0.0 19.178858 12-13 GTATAGG 855 0.0 18.29987 1 CTTTGCG 765 0.0 17.94535 2 AGTACAT 9085 0.0 17.872469 2 AGAGTAC 17695 0.0 17.856033 10-11 TTGCGTT 815 0.0 17.425251 4 GGACCGT 285 8.9160494E-7 16.607952 6 GAGTACA 11415 0.0 16.199024 1 ACTTTTT 12870 0.0 16.166613 16-17 ATGGGAT 3240 0.0 16.071457 5 GTATCAA 31255 0.0 15.458056 1 GTGTAGC 1495 0.0 15.222999 1 >>END_MODULE