FastQCFastQC Report
Fri 27 May 2016
SRR938940_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938940_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2795698
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT162130.579926730283457No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108670.3887043593406727No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90360.3232108761389821No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT74450.2663020111614345No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT59400.21246930104753806No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59240.2118969931659285No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC54120.19358314095442353No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT43770.15656197486280707No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC39650.14182504691136166No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT37570.13438504445043778No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC36690.13123735110158538No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG35010.1252281183446853No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA34310.1227242713626436No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC30010.10734349704438749No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG125950.024.3208261
TACATGG125450.023.754732
ACATGGG125550.022.979893
GAGTACT104150.022.09752512-13
TTTGCGT7200.021.7491633
TTGCGTT7750.020.8179684
CTTTGCG7900.020.422692
GTACTTT113700.020.09541314-15
AGTACTT109250.019.9149412-13
CATGGGA78950.019.8345764
CATGGGG45200.019.6319894
GTATAGG8100.018.232331
GTCTACG1600.001168414817.8647421
AGAGTAC192900.017.79784610-11
ATGGGAT36600.017.7623565
ACTTTTT137750.016.81083716-17
TACACTG20650.016.7750555
AGTACAT98100.015.7692022
TACTTTT139250.015.70966314-15
GTAAGCG2801.8189894E-1215.2526694-95
Produced by FastQC (version 0.11.5)