##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938940_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2795698 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.1248968951582 33.0 31.0 34.0 30.0 34.0 2 32.34740590721888 34.0 31.0 34.0 30.0 34.0 3 32.44560893200911 34.0 31.0 34.0 30.0 34.0 4 36.02809888621732 37.0 35.0 37.0 35.0 37.0 5 35.94644700536324 37.0 35.0 37.0 35.0 37.0 6 35.990781193104546 37.0 35.0 37.0 35.0 37.0 7 35.93020598076044 37.0 35.0 37.0 35.0 37.0 8 35.897850912366074 37.0 35.0 37.0 35.0 37.0 9 37.56581254484569 39.0 37.0 39.0 35.0 39.0 10-11 37.483848040811274 39.0 37.0 39.0 34.5 39.0 12-13 37.41842752686449 39.0 37.0 39.0 34.0 39.0 14-15 38.64939489172292 40.0 38.0 41.0 34.0 41.0 16-17 38.64839836062407 40.0 38.0 41.0 34.0 41.0 18-19 38.765792657146804 40.0 38.0 41.0 34.0 41.0 20-21 38.76589907064354 40.0 38.0 41.0 34.0 41.0 22-23 38.70581675846247 40.0 38.0 41.0 34.0 41.0 24-25 38.64127044480484 40.0 38.0 41.0 34.0 41.0 26-27 38.483228875221855 40.0 38.0 41.0 34.0 41.0 28-29 38.309420044654324 40.0 38.0 41.0 34.0 41.0 30-31 38.14238769709747 40.0 37.5 41.0 33.0 41.0 32-33 37.97654431916466 40.0 37.0 41.0 33.0 41.0 34-35 37.92794321847353 40.0 37.0 41.0 33.0 41.0 36-37 37.813652261438826 40.0 37.0 41.0 33.0 41.0 38-39 37.68253116037569 40.0 37.0 41.0 33.0 41.0 40-41 37.54224848320527 40.0 37.0 41.0 32.5 41.0 42-43 37.36704930217785 40.0 37.0 41.0 32.0 41.0 44-45 37.10111034882881 40.0 36.5 41.0 31.0 41.0 46-47 36.78283956278539 40.0 36.0 41.0 30.5 41.0 48-49 36.48338107334912 39.5 36.0 41.0 30.0 41.0 50-51 36.224106824127645 39.0 35.5 41.0 30.0 41.0 52-53 36.020854362667215 39.0 35.0 41.0 29.0 41.0 54-55 35.789964259372795 39.0 35.0 40.0 28.0 41.0 56-57 35.521036428112055 38.5 35.0 40.0 28.0 41.0 58-59 35.22489106477166 38.0 34.5 40.0 27.5 41.0 60-61 34.81693784521791 38.0 34.0 40.0 26.0 41.0 62-63 34.528251620883225 37.5 34.0 40.0 26.0 41.0 64-65 34.29262817371547 37.0 34.0 40.0 26.0 41.0 66-67 33.83667298828414 36.5 33.0 39.0 25.5 41.0 68-69 33.41729829187559 36.0 33.0 39.0 24.5 40.0 70-71 32.952870624795665 35.5 32.0 38.5 23.5 40.0 72-73 32.43239344879168 35.0 32.0 38.0 22.0 40.0 74-75 31.964421407462467 35.0 31.0 37.0 21.5 39.0 76-77 29.976164807500666 33.0 29.0 35.0 18.5 37.5 78-79 30.952803915158217 34.0 30.5 36.0 19.5 38.0 80-81 30.934045808953613 34.0 31.0 36.0 18.5 37.5 82-83 30.60450932110693 34.0 31.0 35.0 18.0 37.0 84-85 30.18673100599564 34.0 30.0 35.0 13.5 36.5 86-87 29.620918818842377 34.0 29.5 35.0 7.0 36.0 88-89 29.038857737852943 34.0 29.0 35.0 2.0 36.0 90-91 28.556082237781048 34.0 29.0 35.0 2.0 35.0 92-93 28.200029831548328 33.0 29.0 35.0 2.0 35.0 94-95 27.78751406625465 33.0 27.5 35.0 2.0 35.0 96-97 27.148736737659075 33.0 26.5 35.0 2.0 35.0 98-99 26.252486677745594 33.0 25.0 34.5 2.0 35.0 100-101 21.924604696215397 28.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.02196202631630939 1101 2 0.08549908379425375 1101 3 0.12838440613216306 1101 4 0.047715462630613104 1101 5 0.04945390977465536 1101 6 0.10548305437557559 1101 7 0.10101033749663912 1101 8 0.12823215320489112 1101 9 0.19352410652312813 1101 10-11 0.2563676945982394 1101 12-13 0.18328747000789036 1101 14-15 0.2681716583416929 1101 16-17 0.21733781480855896 1101 18-19 0.27968349223934297 1101 20-21 0.2598484063835187 1101 22-23 0.20447746200817818 1101 24-25 0.22124095441230907 1101 26-27 0.09120162100582974 1101 28-29 0.2038998740290694 1101 30-31 0.04054283590767227 1101 32-33 0.058553454309851816 1101 34-35 0.13766134445647538 1101 36-37 0.027355468006682315 1101 38-39 0.049158059786002184 1101 40-41 0.025381850259556415 1101 42-43 -0.0010986841466831265 1101 44-45 -0.013913061309501984 1101 46-47 -0.020973895693749967 1101 48-49 -0.21703005597863267 1101 50-51 -0.2387239343738088 1101 52-53 -0.09383275758676035 1101 54-55 0.12672076818910938 1101 56-57 -0.20675683357807628 1101 58-59 -0.10829563980081502 1101 60-61 0.08203056937518483 1101 62-63 -0.03951761229215123 1101 64-65 -0.179578294631618 1101 66-67 -0.1373914899083175 1101 68-69 -0.14730498658049385 1101 70-71 -0.02538010218184894 1101 72-73 -0.07539246893961149 1101 74-75 -0.1268141555781881 1101 76-77 -0.3765472727437107 1101 78-79 -0.24715559277891685 1101 80-81 -0.03098354944738091 1101 82-83 -0.07141377274873761 1101 84-85 -0.15171740405353873 1101 86-87 -0.16500067244862393 1101 88-89 -0.0024683048415887754 1101 90-91 -0.12251267286421452 1101 92-93 -0.035182377722406954 1101 94-95 -0.08427046779242175 1101 96-97 -0.1684380426322214 1101 98-99 -0.11010467311492178 1101 100-101 0.6196944646168774 1102 1 0.12457280776277457 1102 2 0.11592849795612636 1102 3 0.11287726761225514 1102 4 0.09937148082609326 1102 5 0.06872797158494137 1102 6 0.11977954125084977 1102 7 0.08929421339308874 1102 8 0.12566543239607597 1102 9 0.2018697950242938 1102 10-11 0.23466420237183883 1102 12-13 0.312991334921108 1102 14-15 0.32370286476722754 1102 16-17 0.37309478120752715 1102 18-19 0.17372578580741305 1102 20-21 0.2040719348801332 1102 22-23 0.21078570883599212 1102 24-25 0.3795260377676044 1102 26-27 0.35064302035775086 1102 28-29 0.3583525816974813 1102 30-31 0.297526505026795 1102 32-33 0.2974571687551162 1102 34-35 0.4858975547540041 1102 36-37 0.4076890401227402 1102 38-39 0.46969115867107547 1102 40-41 0.3305364514324154 1102 42-43 0.38295638642580343 1102 44-45 0.40938717913735445 1102 46-47 0.4830423562378954 1102 48-49 0.535318973932668 1102 50-51 0.4569218413198115 1102 52-53 0.5176190317163147 1102 54-55 0.5527647847080814 1102 56-57 0.5495921940111899 1102 58-59 0.5234385555497738 1102 60-61 0.520995556551739 1102 62-63 0.5367308014668453 1102 64-65 0.605416823287193 1102 66-67 0.4822721805575938 1102 68-69 0.6567788656311819 1102 70-71 0.5975416753833329 1102 72-73 0.6612767210064305 1102 74-75 0.6711798663395143 1102 76-77 0.2799346043439428 1102 78-79 0.4389042368068452 1102 80-81 0.5842350180188021 1102 82-83 0.6291836000994628 1102 84-85 0.585845479590386 1102 86-87 0.6945345661928464 1102 88-89 0.8344093749333581 1102 90-91 0.8007238567133008 1102 92-93 0.8258166145138688 1102 94-95 1.0178113247731133 1102 96-97 0.7013410040093575 1102 98-99 0.7093285642796126 1102 100-101 1.139856415034437 1103 1 0.06527843655462817 1103 2 0.10890353199492608 1103 3 0.11112852945186802 1103 4 0.13642185711631072 1103 5 0.09202371287980071 1103 6 0.008904221985936545 1103 7 0.07209060701535464 1103 8 0.1211661370689967 1103 9 0.15642009877046803 1103 10-11 0.18390542013312228 1103 12-13 0.11532154914509363 1103 14-15 0.17286801085386116 1103 16-17 0.11430230098076066 1103 18-19 0.1356479867310938 1103 20-21 0.18861720890600253 1103 22-23 0.1521427404589346 1103 24-25 0.12454041286753181 1103 26-27 0.16634630213645352 1103 28-29 0.24324061921327456 1103 30-31 0.2257470906933392 1103 32-33 0.2910203073916193 1103 34-35 0.19748996476952385 1103 36-37 0.1379179436038953 1103 38-39 0.15801101303677 1103 40-41 0.2962520656983898 1103 42-43 0.2710076286927503 1103 44-45 0.34849498982642046 1103 46-47 0.3110658923702303 1103 48-49 0.2500878884405111 1103 50-51 0.19315867173118306 1103 52-53 0.2072155189915179 1103 54-55 0.3777647989323967 1103 56-57 0.35728362636060496 1103 58-59 0.2247278623302833 1103 60-61 0.33526744479636506 1103 62-63 0.35653538203995083 1103 64-65 0.36172787052161226 1103 66-67 0.4303978558572865 1103 68-69 0.3811138501238318 1103 70-71 0.38510249147251585 1103 72-73 0.5683948847261036 1103 74-75 0.4373224094093331 1103 76-77 0.3969941610116514 1103 78-79 0.462870085916375 1103 80-81 0.4605536838001463 1103 82-83 0.5173914521528573 1103 84-85 0.45259515940089656 1103 86-87 0.29107781410628064 1103 88-89 0.29813344186050017 1103 90-91 0.43086751383203037 1103 92-93 0.3278395264829541 1103 94-95 0.40270234939655225 1103 96-97 0.30867432482528656 1103 98-99 0.44633222789601845 1103 100-101 0.9744476051720881 1104 1 0.03865676426366349 1104 2 0.05678504680557239 1104 3 0.020389871784075808 1104 4 0.10755046604019469 1104 5 0.06577699371969459 1104 6 0.028776737921447193 1104 7 -0.07001662958006705 1104 8 0.10016876174091038 1104 9 0.20441409952037048 1104 10-11 0.04367294919199338 1104 12-13 0.07913857201722863 1104 14-15 0.08362916570950318 1104 16-17 0.08055651920348339 1104 18-19 0.13789770551624514 1104 20-21 0.041823058174841776 1104 22-23 0.03197026201873854 1104 24-25 0.05911109153269223 1104 26-27 0.0967924963621698 1104 28-29 0.08688516364522769 1104 30-31 -0.017784967799343576 1104 32-33 -0.0021930409398365214 1104 34-35 0.0356976888040208 1104 36-37 0.051491246940969404 1104 38-39 0.12838971569893687 1104 40-41 0.061343929215446735 1104 42-43 0.010040249615137498 1104 44-45 0.03184707081629767 1104 46-47 -0.13850291152927952 1104 48-49 -0.27315920567271945 1104 50-51 -0.2789073200198473 1104 52-53 -0.3192186767340104 1104 54-55 -0.28084037542080154 1104 56-57 -0.22276886707771837 1104 58-59 -0.24246373871959293 1104 60-61 -0.11542621849572043 1104 62-63 -0.07409828875120184 1104 64-65 -0.276067405068936 1104 66-67 -0.11140941823345685 1104 68-69 -0.0782037351780076 1104 70-71 -0.2776271673201336 1104 72-73 -0.4512901546439707 1104 74-75 -0.3133337585696694 1104 76-77 -0.3988438592358179 1104 78-79 -0.27203805056656805 1104 80-81 -0.05294462891076179 1104 82-83 -0.09696925275902757 1104 84-85 -0.18913712406367367 1104 86-87 -0.303972804566385 1104 88-89 -0.08844323605476134 1104 90-91 0.008295335354343791 1104 92-93 -0.12641619155229478 1104 94-95 -0.16328940163456807 1104 96-97 -0.14483148694824166 1104 98-99 -0.10409896820446818 1104 100-101 0.7271660153708126 1105 1 -0.04733842556412782 1105 2 -0.03197278111786517 1105 3 -0.09117960744538323 1105 4 -0.11560796512276283 1105 5 0.006769314430982831 1105 6 -0.012242720281633979 1105 7 -0.0489185762289992 1105 8 -0.02333914231251555 1105 9 -0.09047182979371371 1105 10-11 -0.02050742605116085 1105 12-13 -0.04597189414687364 1105 14-15 -0.035571749093271876 1105 16-17 -0.03060941930834815 1105 18-19 -0.04885754123813513 1105 20-21 -0.026786528610230675 1105 22-23 -0.11118497893525614 1105 24-25 -0.09438456957595776 1105 26-27 -0.014396229356506751 1105 28-29 -0.01928996225409918 1105 30-31 -0.08637321747255555 1105 32-33 -0.07155253398662609 1105 34-35 -0.014054448532405672 1105 36-37 0.0980902563189403 1105 38-39 0.08281033024919537 1105 40-41 0.14114679916057327 1105 42-43 -0.0011441258185982406 1105 44-45 0.036802873992826335 1105 46-47 0.07849489468416238 1105 48-49 0.006926018480889695 1105 50-51 0.008136081322945188 1105 52-53 0.12757153802505172 1105 54-55 0.18282749482469995 1105 56-57 0.04770222735039198 1105 58-59 0.023411797556605052 1105 60-61 0.013853477895814592 1105 62-63 -0.1015204134718033 1105 64-65 -0.1670308274588237 1105 66-67 -0.037855629031689375 1105 68-69 -0.2030463480834328 1105 70-71 -0.1302067827135076 1105 72-73 0.0012333213579864832 1105 74-75 0.07061990468098855 1105 76-77 -0.10108922773602558 1105 78-79 0.03628363021780956 1105 80-81 0.11085188547521341 1105 82-83 0.12235388433501981 1105 84-85 -0.12563684336671344 1105 86-87 -0.1241966578002014 1105 88-89 0.008714784938472775 1105 90-91 0.3285722206331876 1105 92-93 0.21839920099877475 1105 94-95 0.05637657934432738 1105 96-97 0.04250276201649328 1105 98-99 0.07724887436493333 1105 100-101 0.46312049896091523 1106 1 -0.1040852844046185 1106 2 -0.12680343268012706 1106 3 -0.10543484915046264 1106 4 -0.0566606509268297 1106 5 -0.08926757491791193 1106 6 -0.08253162494099797 1106 7 -6.228378489367969E-4 1106 8 -0.08692636443419843 1106 9 -0.11622727043720005 1106 10-11 -0.005588536662948229 1106 12-13 -0.03944922228544101 1106 14-15 0.024690659817004246 1106 16-17 -0.05297599331784397 1106 18-19 -0.11914149785533112 1106 20-21 -0.09396358921561898 1106 22-23 0.047548688289246854 1106 24-25 0.01304284840684744 1106 26-27 -0.047697976557643074 1106 28-29 0.00936957245371417 1106 30-31 0.006684001551512608 1106 32-33 -0.009297623054784765 1106 34-35 -0.0723477400039485 1106 36-37 -0.07962904870480969 1106 38-39 0.020217393477715007 1106 40-41 0.04382491003874378 1106 42-43 0.09610117032173093 1106 44-45 0.14561953954110152 1106 46-47 0.1681960532716289 1106 48-49 0.051268542294188535 1106 50-51 0.06278497028412744 1106 52-53 0.14105285170991522 1106 54-55 0.15540144602615413 1106 56-57 0.058480757727643606 1106 58-59 0.12239643219113816 1106 60-61 0.11075552902875074 1106 62-63 0.07627778910104155 1106 64-65 -0.02011172930389904 1106 66-67 0.08280698837416622 1106 68-69 0.05771222067164672 1106 70-71 -0.00975882900007008 1106 72-73 0.08349899597609323 1106 74-75 0.050131688816208 1106 76-77 -0.08963186664214362 1106 78-79 0.1382081474956287 1106 80-81 0.12403807894637353 1106 82-83 0.18509058545497226 1106 84-85 0.17755993078091947 1106 86-87 0.28651158326570325 1106 88-89 0.4346613013473295 1106 90-91 0.7016350112882996 1106 92-93 0.6199622400727165 1106 94-95 0.5610439387368977 1106 96-97 0.5640203739964527 1106 98-99 0.642273943315562 1106 100-101 0.9767582521159746 1107 1 -0.012675402248014223 1107 2 -0.025838921227304468 1107 3 -0.02768358654265768 1107 4 -0.020880185904488258 1107 5 -0.06512103933303592 1107 6 -0.04051392385052566 1107 7 -0.052876398462750274 1107 8 0.04251707830964335 1107 9 -0.005952745418234429 1107 10-11 0.07496265260000001 1107 12-13 -0.076257910462175 1107 14-15 -0.003024262539760514 1107 16-17 0.03909292958901034 1107 18-19 -0.019865313276085317 1107 20-21 -0.015704227448750885 1107 22-23 0.05557775783265839 1107 24-25 -0.050446626207452994 1107 26-27 -0.055902834591499584 1107 28-29 0.02407421988683467 1107 30-31 -0.09846608302095206 1107 32-33 -0.026595383246323934 1107 34-35 -0.05458838554498868 1107 36-37 -0.04315240545446386 1107 38-39 -0.061968642033413346 1107 40-41 -0.09320274974209752 1107 42-43 -0.1860085971671026 1107 44-45 -0.21896997506740945 1107 46-47 -0.40381690431989625 1107 48-49 -0.4846218071435686 1107 50-51 -0.3188572901724953 1107 52-53 -0.24416490412346548 1107 54-55 -0.4543406644189787 1107 56-57 -0.21760649342136418 1107 58-59 -0.23231584204421551 1107 60-61 -0.26023352371975506 1107 62-63 -0.3267416406426875 1107 64-65 -0.40433129085577946 1107 66-67 -0.18285233804898127 1107 68-69 -0.17029242716009918 1107 70-71 -0.27260029116701645 1107 72-73 -0.23326648251273951 1107 74-75 -0.3673144884152606 1107 76-77 -0.5147840372911787 1107 78-79 -0.28211163342647083 1107 80-81 -0.2123468832318629 1107 82-83 -0.12095677778410518 1107 84-85 -0.022530355144443348 1107 86-87 -0.19293564630503113 1107 88-89 -0.016769388426506993 1107 90-91 0.1506188129701833 1107 92-93 0.21994662771623652 1107 94-95 0.2260456859921156 1107 96-97 0.075803185522755 1107 98-99 -0.0981083136066907 1107 100-101 0.6997554525214973 1108 1 0.0034975919194550897 1108 2 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-3.007872645264989 2316 98-99 -3.447356343837942 2316 100-101 -4.11813663627737 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 8.0 4 21.0 5 70.0 6 141.0 7 322.0 8 857.0 9 1562.0 10 2652.0 11 4233.0 12 6258.0 13 8700.0 14 10615.0 15 13842.0 16 17860.0 17 25220.0 18 24325.0 19 15898.0 20 14603.0 21 15227.0 22 16776.0 23 18372.0 24 21823.0 25 25612.0 26 31110.0 27 37881.0 28 46254.0 29 56476.0 30 68716.0 31 83660.0 32 103856.0 33 131801.0 34 175394.0 35 248052.0 36 386786.0 37 623261.0 38 514572.0 39 42880.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.28629855675612 20.61566098816918 14.757892302244416 22.340148152830285 2 15.261591201910937 21.910628401207855 39.67721120092371 23.150569195957505 3 19.350051400401615 25.884984715802638 30.832586352317026 23.93237753147872 4 12.59535185846254 17.500745788708223 38.25799496225987 31.645907390569366 5 12.648787008292404 38.59468017030495 34.98730725248248 13.76922556892017 6 30.305526562597247 37.60456243843219 17.6016150528419 14.488295946128659 7 26.896288511849274 33.8154550312659 21.879258775447134 17.408997681437693 8 25.240208348684302 35.390088629029314 20.271073628124352 19.09862939416203 9 25.741800437672453 17.306697647599993 21.740009114003016 35.21149280072454 10-11 24.451568087826367 26.96927565137579 27.897183458299146 20.681972802498695 12-13 25.800122187732722 24.749025109292923 28.15475777426603 21.296094928708325 14-15 22.307356072602456 25.97044064323919 27.38303937205593 24.339163912102425 16-17 21.104949925562607 29.181657281808306 27.985883723142983 21.727509069486107 18-19 20.96798366633306 28.219750488071316 30.03546878096275 20.776797064632877 20-21 22.143557709022936 26.86842427186341 29.7500838788739 21.23793414023975 22-23 21.53859250891906 27.032301056838044 29.77955773477679 21.64954869946611 24-25 21.821598756374975 27.187843608286734 29.850881604522378 21.139676030815917 26-27 21.639229272975836 26.889134663329155 30.401209286553843 21.070426777141165 28-29 21.00772329486232 27.82489381900334 30.051725901724723 21.11565698440962 30-31 21.384802650357802 27.12338743312046 29.87343411198205 21.61837580453969 32-33 21.337211673077707 27.28980383431973 29.93445643985867 21.438528052743894 34-35 22.02895476575728 27.10132379513574 29.73195907264768 21.1377623664593 36-37 21.86251175771672 26.77280612497673 30.083693301155478 21.280988816151076 38-39 21.39644542400615 27.46075676627061 30.04093040793216 21.10186740179108 40-41 21.515324078661255 27.405206620468427 29.778443737071353 21.301025563798966 42-43 21.290613928250142 27.530980779308113 29.258929385821364 21.91947590662038 44-45 21.699613833875596 27.23075679403424 29.137460975616737 21.932168396473426 46-47 22.07507516476693 27.709870706406626 28.80811420001098 21.406939928815465 48-49 21.93503825439967 27.65412421220296 28.75823387523266 21.652603658164715 50-51 21.28221953593817 27.887455786751254 29.110609266034587 21.719715411275992 52-53 21.8472810725622 28.25605626931092 28.207392214752808 21.68927044337407 54-55 21.353200524520176 27.655329724455218 28.609510040068702 22.381959710955904 56-57 21.837247800012737 27.85127363542128 28.335571295612045 21.975907268953943 58-59 22.31620801199845 27.564492472264046 28.342295626364038 21.777003889373464 60-61 21.81507664555573 27.749055355530967 28.92852179751351 21.507346201399795 62-63 21.49355903248491 27.76966610842802 28.900063597713345 21.836711261373726 64-65 21.311904641822004 28.091140973734223 28.61972227975123 21.977232104692543 66-67 21.538512441628168 27.782845165086535 28.11881294365721 22.559829449628083 68-69 21.283818949306767 27.756389459515628 28.555968378117647 22.40382321305996 70-71 21.784702782632458 27.637248372320617 28.308583402069893 22.269465442977033 72-73 21.718261414501853 27.198717458037315 28.75462585729932 22.328395270161515 74-75 21.179719697907284 28.359089572621937 28.06708736065197 22.394103368818808 76-77 21.38334214060124 27.610883383595347 28.276093254731077 22.729681221072333 78-79 21.50573346009971 27.631457520029016 28.34578820142691 22.517020818444365 80-81 21.561699439639046 27.76179687505589 28.198092211676656 22.47841147362841 82-83 21.481755182426713 27.632240678356535 28.4829405751265 22.403063564090257 84-85 21.198641627243 27.71760397582285 28.260938770925904 22.822815626008243 86-87 21.134563211862545 27.72397604006195 28.291558551234402 22.849902196841104 88-89 21.2405891115992 27.60164998959011 27.945562486897824 23.212198411912862 90-91 21.26548136583265 27.315289124573233 28.207647478431742 23.211582031162376 92-93 21.493058263088503 27.640932604308478 28.145332578840776 22.720676553762246 94-95 21.20933663078058 27.281773639355894 28.18705740033437 23.321832329529155 96-97 20.764671680467405 27.321297278465178 28.53856668682274 23.375464354244677 98-99 20.957038278097276 27.4982490955747 27.80988862173239 23.73482400459563 100-101 20.923121111983285 26.783696830424127 28.615409543871017 23.67777251372157 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 982.0 1 660.5 2 432.0 3 541.0 4 499.5 5 531.0 6 856.0 7 1646.5 8 2691.0 9 3280.0 10 3095.5 11 2849.0 12 2980.0 13 3170.0 14 3477.5 15 3757.0 16 4036.0 17 4391.0 18 4679.5 19 5100.0 20 5915.5 21 6884.0 22 8139.0 23 9968.5 24 12094.5 25 14930.5 26 19051.0 27 23484.5 28 26694.0 29 30644.5 30 36216.5 31 42155.5 32 48289.0 33 55116.0 34 62624.0 35 68590.0 36 75966.5 37 83661.5 38 86802.0 39 87422.5 40 88144.5 41 90290.5 42 91856.5 43 94212.0 44 97432.5 45 99725.0 46 101040.0 47 111364.0 48 137392.0 49 135788.0 50 109437.0 51 95205.5 52 86324.5 53 82189.5 54 78413.5 55 70896.0 56 62815.5 57 55748.0 58 50173.5 59 44017.5 60 37014.5 61 30162.5 62 24102.5 63 18633.0 64 13851.5 65 10327.0 66 7379.5 67 5656.0 68 4134.0 69 2540.5 70 1699.5 71 1181.0 72 840.0 73 623.5 74 449.0 75 335.0 76 211.0 77 93.0 78 55.0 79 44.0 80 33.0 81 20.0 82 11.5 83 7.0 84 3.5 85 4.5 86 3.5 87 1.5 88 0.5 89 0.5 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.40333397956431627 2 0.0 3 0.0 4 0.0 5 2.503846982041694E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.001251923491020847 16-17 0.002146154556035738 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.002199808419936631 36-37 0.006921348443215254 38-39 0.012286734833304598 40-41 0.0023250007690387158 42-43 4.650001538077432E-4 44-45 3.5769242600595626E-4 46-47 2.325000769038716E-4 48-49 2.325000769038716E-4 50-51 5.186540177086367E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 1.251923491020847E-4 60-61 0.0024859623607413964 62-63 0.0 64-65 2.503846982041694E-4 66-67 0.0015380774318256123 68-69 0.0013950004614232295 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.1250671567529826 78-79 1.4307697040238254E-4 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.14742293337835488 88-89 0.4394787992122182 90-91 0.33928915068794985 92-93 0.0 94-95 0.0 96-97 5.186540177086367E-4 98-99 0.0 100-101 3.040385621050628E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2795698.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.44813618410154 #Duplication Level Percentage of deduplicated Percentage of total 1 69.25437202673362 30.78227759188688 2 15.312102459860686 13.611888308016079 3 6.113884407549922 8.152523002819018 4 2.9925437937141353 5.320519763195751 5 1.698275019463336 3.7742579671582024 6 1.087113677529714 2.899210607186411 7 0.6876802802268212 2.1396274722650004 8 0.4712651951546477 1.6757487658448775 9 0.36208780031764787 1.448471507320852 >10 1.7922457637946623 14.212023352511002 >50 0.13979009790968563 4.265587545379545 >100 0.08243691028361291 6.5535695396279685 >500 0.0037054299121168205 1.0641732940406738 >1k 0.0019332677802101148 1.9218994037054233 >5k 4.0276412087710727E-4 1.2086433580029803 >10k+ 1.6110564835084289E-4 0.9695785210392127 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 16213 0.579926730283457 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10867 0.3887043593406727 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9036 0.3232108761389821 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 7445 0.2663020111614345 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5940 0.21246930104753806 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5924 0.2118969931659285 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 5412 0.19358314095442353 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 4377 0.15656197486280707 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3965 0.14182504691136166 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3757 0.13438504445043778 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3669 0.13123735110158538 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 3501 0.1252281183446853 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 3431 0.1227242713626436 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 3001 0.10734349704438749 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 3.576924260059563E-5 0.0 4 0.0 0.0 0.0 3.576924260059563E-5 0.0 5 0.0 0.0 0.0 3.576924260059563E-5 0.0 6 3.576924260059563E-5 0.0 0.0 3.576924260059563E-5 0.0 7 3.576924260059563E-5 0.0 0.0 7.153848520119126E-5 0.0 8 3.576924260059563E-5 0.0 0.0 7.153848520119126E-5 0.0 9 3.576924260059563E-5 0.0 0.0 1.430769704023825E-4 0.0 10-11 7.153848520119126E-5 0.0 0.0 1.9673083430327598E-4 0.0 12-13 1.251923491020847E-4 0.0 0.0 3.755770473062541E-4 0.0 14-15 2.1461545560357378E-4 0.0 0.0 4.4711553250744536E-4 0.0 16-17 2.861539408047651E-4 0.0 0.0 5.186540177086367E-4 0.0 18-19 4.8288477510804106E-4 0.0 0.0 6.080771242101257E-4 0.0 20-21 6.080771242101257E-4 0.0 0.0 6.975002307116147E-4 0.0 22-23 6.438463668107213E-4 0.0 0.0 0.001180385005819656 0.0 24-25 8.942310650148907E-4 0.0 0.0 0.0016275005383271013 0.0 26-27 9.300003076154864E-4 0.0 0.0 0.0022713469051378224 0.0 28-29 0.0011982696271199537 0.0 0.0 0.005544232603092322 0.0 30-31 0.0013950004614232295 0.0 0.0 0.015434428182157013 0.0 32-33 0.0014128850827235273 0.0 0.0 0.03292558781384827 0.0 34-35 0.0014844235679247187 0.0 0.0 0.05991348135599768 0.0 36-37 0.0017169236448285902 0.0 0.0 0.10217484148860141 0.0 38-39 0.001734808266128888 0.0 0.0 0.17296217259518015 0.0 40-41 0.0017526928874291858 0.0 0.0 0.2528885451862111 0.0 42-43 0.0018778852365312706 0.0 0.0 0.3373397269662174 0.0 44-45 0.002110385313435142 0.0 0.0 0.43588399033085834 0.0 46-47 0.002360770011639312 0.0 0.0 0.5553890298594484 0.0 48-49 0.002503846982041694 0.0 0.0 0.6840331108724905 0.0 50-51 0.002503846982041694 0.0 0.0 0.831491813493446 0.0 52-53 0.002521731603341992 0.0 0.0 0.9759995535998524 0.0 54-55 0.0025753854672428854 0.0 0.0 1.1341711443796862 0.0 56-57 0.0027184624376452677 0.0 0.0 1.3285948625352237 0.0 58-59 0.0027184624376452677 0.0 0.0 1.5432997412452991 0.0 60-61 0.0027184624376452677 0.0 0.0 1.783758474627803 0.0 62-63 0.0027363470589455655 0.0 0.0 2.026041439382938 0.0 64-65 0.0028615394080476505 0.0 0.0 2.2768195992557136 0.0 66-67 0.003004616378450033 0.0 0.0 2.5655310409064214 0.0 68-69 0.003076154863651224 0.0 0.0 2.9021374983993264 0.0 70-71 0.0032013472127533087 0.0 0.0 3.2694876199074434 0.0 72-73 0.0033265395618553937 0.0 0.0 3.6380896649065813 0.0 74-75 0.0034159626683568827 0.0 0.0 4.027366332128864 0.0 76-77 0.0034338472896571805 0.0 0.0 4.435547044065561 0.0 78-79 0.0034338472896571805 0.0 0.0 4.918092011368897 0.0 80-81 0.003791539715663137 0.0 0.0 5.461623537306247 0.0 82-83 0.0038988474434649236 0.0 0.0 6.002114677622547 0.0 84-85 0.003970385928666115 0.0 0.0 6.542462740968444 0.0 86-87 0.004059809035167604 0.0 0.0 7.1082963896672675 0.0 88-89 0.004077693656467902 0.0 0.0 7.722615246711197 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 12595 0.0 24.320826 1 TACATGG 12545 0.0 23.75473 2 ACATGGG 12555 0.0 22.97989 3 GAGTACT 10415 0.0 22.097525 12-13 TTTGCGT 720 0.0 21.749163 3 TTGCGTT 775 0.0 20.817968 4 CTTTGCG 790 0.0 20.42269 2 GTACTTT 11370 0.0 20.095413 14-15 AGTACTT 10925 0.0 19.91494 12-13 CATGGGA 7895 0.0 19.834576 4 CATGGGG 4520 0.0 19.631989 4 GTATAGG 810 0.0 18.23233 1 GTCTACG 160 0.0011684148 17.864742 1 AGAGTAC 19290 0.0 17.797846 10-11 ATGGGAT 3660 0.0 17.762356 5 ACTTTTT 13775 0.0 16.810837 16-17 TACACTG 2065 0.0 16.775055 5 AGTACAT 9810 0.0 15.769202 2 TACTTTT 13925 0.0 15.709663 14-15 GTAAGCG 280 1.8189894E-12 15.25266 94-95 >>END_MODULE