FastQCFastQC Report
Fri 27 May 2016
SRR938939_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938939_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3846414
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT356000.9255373966504906No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT200730.5218627012068904No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT156340.40645650728184746No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC137590.35770980450882306No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT110310.28678660175425735No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT91150.2369739710805961No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC87770.22818656546071225No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85660.22270093650865455No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC84350.21929516687491257No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG74750.19433685505512407No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA70480.18323560594361396No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC70320.18281963407995083No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67380.1751761510851406No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA57170.14863194653513637No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT46460.12078782991118481No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA45330.11785002862406387No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC43800.11387229767778506No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42100.10945259662636418No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC39790.10344700284472758No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAAGCG6100.024.17169894-95
TTTGCGT10050.020.2649483
GTACATG106900.019.9809931
TACATGG105400.019.5475222
GAGTACT92050.018.92793512-13
AGAGTAC148350.018.38290610-11
GTACTTT94250.018.16030314-15
ACATGGG107700.018.0746213
AGTACTT96950.017.72711212-13
CATGGGG68400.017.4496824
GTGTAAG15950.015.1772041
CTTTGCG13450.015.1422112
GAGTACA92400.015.0000661
GTATATA21950.014.9209611
AGTACAT83800.014.7516162
ACTTTTT120200.014.53623616-17
TACTTTT116200.014.52611814-15
GTGTAGC22800.014.3646971
GTATAGG8500.013.9605761
TATACTG17400.013.8823825
Produced by FastQC (version 0.11.5)