FastQCFastQC Report
Fri 27 May 2016
SRR938939_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938939_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3846414
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT380830.9900910302427143No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT185430.4820853917441024No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT149410.3884397259369376No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC125020.32503001496978745No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT114190.2968739194480885No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC97120.2524949212435271No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC95350.2478932325017536No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT86580.22509277472471761No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85660.22270093650865455No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG84730.22028310005111257No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA81490.2118596698119339No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71390.18560144591819808No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC68800.17886790137515096No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA63740.16571279118680413No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT48190.12528552568704254No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC46810.12169776836294793No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45430.11811001103885334No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA45330.11785002862406387No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC38620.10040520859169086No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAAGCG8300.026.58724294-95
TTTGCGT10100.023.4931583
GAGTACT98950.019.49614712-13
GTACATG103250.019.2877521
TACATGG101200.019.0387442
GTACTTT101100.018.87030614-15
CTTTGCG13550.018.2119662
AGAGTAC159350.017.9733610-11
AGTACTT105950.017.46898712-13
ACATGGG106000.017.1469023
TTGCGTT14650.016.8445154
CGTATAC1750.002184407816.2706893
ACTTTTT127450.015.28512416-17
CATGGGG70800.015.0814134
TACTTTT122350.014.89468414-15
TCGTATA1950.004491435414.6019012
GTGTAGC22050.014.2603681
GTCTACG2701.6363178E-414.1163241
GTATATA23300.013.6998021
CTACACT16450.013.5589094
Produced by FastQC (version 0.11.5)