##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938939_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3846414 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.1719918864688 33.0 31.0 34.0 30.0 34.0 2 32.41585565152373 34.0 31.0 34.0 31.0 34.0 3 32.51401279217474 34.0 31.0 34.0 31.0 34.0 4 36.0495601877489 37.0 35.0 37.0 35.0 37.0 5 35.97320465243731 37.0 35.0 37.0 35.0 37.0 6 36.01673350814551 37.0 35.0 37.0 35.0 37.0 7 35.955968338301595 37.0 35.0 37.0 35.0 37.0 8 35.92663660230022 37.0 35.0 37.0 35.0 37.0 9 37.60372414409889 39.0 37.0 39.0 35.0 39.0 10-11 37.505890421571884 39.0 37.0 39.0 35.0 39.0 12-13 37.419952844389606 39.0 37.0 39.0 34.0 39.0 14-15 38.723515851387816 40.0 38.0 41.0 34.5 41.0 16-17 38.83432503625455 40.0 38.0 41.0 35.0 41.0 18-19 38.861515427096506 40.0 38.0 41.0 35.0 41.0 20-21 38.82093763177859 40.0 38.0 41.0 34.0 41.0 22-23 38.744946721803736 40.0 38.0 41.0 34.0 41.0 24-25 38.66105104650721 40.0 38.0 41.0 34.0 41.0 26-27 38.49278951771702 40.0 38.0 41.0 34.0 41.0 28-29 38.34746818725181 40.0 38.0 41.0 34.0 41.0 30-31 38.186607187889805 40.0 38.0 41.0 33.0 41.0 32-33 38.11336975166999 40.0 38.0 41.0 33.0 41.0 34-35 38.00668440786666 40.0 38.0 41.0 33.0 41.0 36-37 37.87927352593871 40.0 37.0 41.0 33.0 41.0 38-39 37.7401786443165 40.0 37.0 41.0 33.0 41.0 40-41 37.613177364683054 40.0 37.0 41.0 32.5 41.0 42-43 37.43184275015638 40.0 37.0 41.0 32.0 41.0 44-45 37.20015162174431 40.0 36.5 41.0 31.0 41.0 46-47 36.94084087152345 40.0 36.0 41.0 31.0 41.0 48-49 36.626259939777675 39.0 36.0 41.0 30.0 41.0 50-51 36.36175032120828 39.0 35.0 40.5 30.0 41.0 52-53 36.11266584928195 39.0 35.0 40.0 29.5 41.0 54-55 35.810785578463474 38.5 35.0 40.0 28.5 41.0 56-57 35.63013679754701 38.0 35.0 40.0 28.0 41.0 58-59 35.32199407032108 38.0 34.0 40.0 28.0 41.0 60-61 34.89581919678953 38.0 34.0 40.0 27.0 41.0 62-63 34.466589139910575 37.0 34.0 40.0 26.0 41.0 64-65 34.04480081967256 36.5 33.0 39.0 26.0 41.0 66-67 33.5446521097313 36.0 32.5 39.0 25.5 40.0 68-69 33.00316086619901 35.5 32.0 38.5 23.5 40.0 70-71 32.419979102613496 35.0 31.0 37.5 22.5 40.0 72-73 31.86438134324594 35.0 31.0 37.0 21.5 39.0 74-75 31.323711904126803 34.5 30.5 36.5 20.0 39.0 76-77 29.437990814301322 32.5 28.5 34.5 18.0 36.5 78-79 30.413308343823623 34.0 30.0 35.0 18.0 37.0 80-81 30.36599505409454 34.0 30.0 35.0 18.0 37.0 82-83 29.919429499788635 34.0 29.5 35.0 13.0 36.5 84-85 29.42190362243898 34.0 29.0 35.0 7.0 36.0 86-87 28.71469347813314 33.5 29.0 35.0 2.0 35.5 88-89 27.96889999360443 33.0 27.0 35.0 2.0 35.0 90-91 27.32386282391859 32.5 26.0 34.5 2.0 35.0 92-93 26.862737214454814 32.0 25.0 34.0 2.0 35.0 94-95 26.229623358276044 32.0 24.5 34.0 2.0 35.0 96-97 25.252932601638825 32.0 21.5 34.0 2.0 35.0 98-99 23.902741878539334 31.0 7.5 34.0 2.0 35.0 100-101 18.151700518976895 23.5 2.0 30.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.15158661562783493 1101 2 0.17693385181782162 1101 3 0.21032752281985267 1101 4 0.10477879438068527 1101 5 0.14417260605707582 1101 6 0.11202180179583365 1101 7 0.24753400736360476 1101 8 0.14529385317919008 1101 9 0.1816188476477265 1101 10-11 0.2691786875440769 1101 12-13 0.20073930463067313 1101 14-15 0.2844377869838226 1101 16-17 0.2702017970560817 1101 18-19 0.2715386178821646 1101 20-21 0.3615916634762968 1101 22-23 0.29151116344876726 1101 24-25 0.16464769908127863 1101 26-27 0.1693527412793756 1101 28-29 0.14854854078483015 1101 30-31 0.15748725574714229 1101 32-33 0.23039797641654758 1101 34-35 0.285872625585057 1101 36-37 0.3500043160736652 1101 38-39 0.33515861757623355 1101 40-41 0.27645273415834026 1101 42-43 0.34628509506102034 1101 44-45 0.2943243812333023 1101 46-47 0.3190704130577373 1101 48-49 0.3153869915759486 1101 50-51 0.2282987568329915 1101 52-53 0.0809840141431124 1101 54-55 0.15585418030157427 1101 56-57 0.17479466800860877 1101 58-59 0.1825069725361388 1101 60-61 0.18902246571001058 1101 62-63 0.2659625181883598 1101 64-65 0.349064984488642 1101 66-67 0.3547614268511907 1101 68-69 0.26117907738234436 1101 70-71 0.19365369452437875 1101 72-73 0.35100443025639194 1101 74-75 0.4106694216183442 1101 76-77 0.021309546575771776 1101 78-79 0.3912122822182056 1101 80-81 0.5147838086819512 1101 82-83 0.3769024404610839 1101 84-85 0.4268064880809561 1101 86-87 0.6148801174158116 1101 88-89 0.5923104691649854 1101 90-91 0.6185091918791095 1101 92-93 0.4161625223005494 1101 94-95 0.38941502500438574 1101 96-97 0.6054556378362825 1101 98-99 0.9047145441142135 1101 100-101 1.306591644381978 1102 1 0.17023325411219048 1102 2 0.16376432535120955 1102 3 0.18209236958498565 1102 4 0.11944940355713385 1102 5 0.11162326751922791 1102 6 0.1649868327068944 1102 7 0.1805848302904849 1102 8 0.16222536509098262 1102 9 0.17926396863326488 1102 10-11 0.24873880718342178 1102 12-13 0.20925652945167172 1102 14-15 0.3251759091950177 1102 16-17 0.33370371008797406 1102 18-19 0.2279319986213153 1102 20-21 0.2292945144469698 1102 22-23 0.211608169174184 1102 24-25 0.32831916593997335 1102 26-27 0.21301820475888178 1102 28-29 0.26146298311491023 1102 30-31 0.30234118211177474 1102 32-33 0.3079692883531564 1102 34-35 0.31406075102792386 1102 36-37 0.4249444508047233 1102 38-39 0.419730964044966 1102 40-41 0.4649194259117877 1102 42-43 0.4477645671919035 1102 44-45 0.45954843931937006 1102 46-47 0.6360047402926554 1102 48-49 0.6907873325076181 1102 50-51 0.619965638283702 1102 52-53 0.6046182156401514 1102 54-55 0.7136901960562767 1102 56-57 0.6288480393977096 1102 58-59 0.5252896537785077 1102 60-61 0.608630279302318 1102 62-63 0.6111966717852511 1102 64-65 0.7174076275536478 1102 66-67 0.6538430367243251 1102 68-69 0.5039772230757151 1102 70-71 0.5478790781914569 1102 72-73 0.7096798088281311 1102 74-75 0.8043940663622351 1102 76-77 0.5080347825375178 1102 78-79 0.7279329779757511 1102 80-81 0.7974496262577375 1102 82-83 0.8561574345865743 1102 84-85 1.0237393323146797 1102 86-87 1.0959708608316205 1102 88-89 0.909723163417322 1102 90-91 0.9972784039811913 1102 92-93 1.0808095977547794 1102 94-95 1.2648321785699537 1102 96-97 1.470768319122275 1102 98-99 1.5615276855667517 1102 100-101 1.5873309878882758 1103 1 0.12570545216396312 1103 2 0.1139236670633963 1103 3 0.11929830598651847 1103 4 0.09201302244380116 1103 5 0.10166052316647267 1103 6 0.13425555712505144 1103 7 0.12426746934734467 1103 8 0.1450206223779773 1103 9 0.15345862153691314 1103 10-11 0.1495164324343321 1103 12-13 0.11918597617703597 1103 14-15 0.15408834114691672 1103 16-17 0.144587008186555 1103 18-19 0.1605742360333977 1103 20-21 0.17915543402691725 1103 22-23 0.1244260672771631 1103 24-25 0.14716585863742182 1103 26-27 0.1726403159541121 1103 28-29 0.12284653094569364 1103 30-31 0.19001901814294797 1103 32-33 0.1788677117419084 1103 34-35 0.2579067369361141 1103 36-37 0.28618895748152084 1103 38-39 0.19016479129310682 1103 40-41 0.2720214799984433 1103 42-43 0.2771252605992842 1103 44-45 0.26700267078813766 1103 46-47 0.3526722446354782 1103 48-49 0.22131689828770362 1103 50-51 0.18159616969498416 1103 52-53 0.31104587497400615 1103 54-55 0.3693609626241994 1103 56-57 0.3390445739314387 1103 58-59 0.28775796605999204 1103 60-61 0.33813068138687186 1103 62-63 0.3883774307826826 1103 64-65 0.38041675141767683 1103 66-67 0.43290844928507255 1103 68-69 0.4754473604441358 1103 70-71 0.5057241641213324 1103 72-73 0.4780193802311281 1103 74-75 0.4680217486274785 1103 76-77 0.36907463445731636 1103 78-79 0.6408846948454006 1103 80-81 0.5805605042156508 1103 82-83 0.6714327996824956 1103 84-85 0.6946251905610232 1103 86-87 0.7160694736399016 1103 88-89 0.6369703606052468 1103 90-91 0.6145469147919265 1103 92-93 0.8066927309358647 1103 94-95 1.047709305612969 1103 96-97 0.8864464755645827 1103 98-99 1.0507777743080702 1103 100-101 1.226373241320914 1104 1 0.0385298445334783 1104 2 0.06824667955492458 1104 3 0.05671599747484635 1104 4 0.028535608867734652 1104 5 0.018213612156500858 1104 6 0.08510587667574043 1104 7 0.03712151698945121 1104 8 0.031033622281142925 1104 9 0.08544153041654567 1104 10-11 0.10945276437971785 1104 12-13 0.06748957354316332 1104 14-15 0.08191860404998863 1104 16-17 0.10700984395686675 1104 18-19 0.1007168839466317 1104 20-21 0.11466429429611935 1104 22-23 0.11049898293514815 1104 24-25 0.09760766167799773 1104 26-27 0.020299970258363942 1104 28-29 0.07765522417703608 1104 30-31 0.11748766828335278 1104 32-33 0.19547254615655874 1104 34-35 0.17860339375803846 1104 36-37 0.19133081862221957 1104 38-39 0.21615945334404074 1104 40-41 0.29984326996648747 1104 42-43 0.2559635001654712 1104 44-45 0.26694729410565543 1104 46-47 0.2463245032778758 1104 48-49 0.15604170946229345 1104 50-51 0.2223325282695967 1104 52-53 0.2377698146467111 1104 54-55 0.48729604783771663 1104 56-57 0.25707134840611445 1104 58-59 0.27712322218471996 1104 60-61 0.23264354814391908 1104 62-63 0.25077515035273734 1104 64-65 0.29918309207808846 1104 66-67 0.10129562610312348 1104 68-69 0.14524860959684105 1104 70-71 0.19957160287411568 1104 72-73 0.2881530105206558 1104 74-75 0.3044596057011688 1104 76-77 0.0763652924412419 1104 78-79 0.3939860916692588 1104 80-81 0.39528222882105624 1104 82-83 0.38788633168597997 1104 84-85 0.45984288447019495 1104 86-87 0.44632481158896553 1104 88-89 0.48603682472841925 1104 90-91 0.7466877833888113 1104 92-93 0.6511741267913855 1104 94-95 0.9407733004807888 1104 96-97 0.9194249543096227 1104 98-99 1.1614736443574536 1104 100-101 1.1048892067513414 1105 1 -0.01092667720565288 1105 2 0.014985809989703114 1105 3 0.047476867040067816 1105 4 0.02799213060686867 1105 5 0.012235351286932428 1105 6 -0.0015789059329520683 1105 7 -0.001465439532289281 1105 8 0.04896840488984111 1105 9 0.061528486938286164 1105 10-11 0.09002341655362756 1105 12-13 0.13515261702142567 1105 14-15 0.08762512578911696 1105 16-17 -0.003180373434439332 1105 18-19 0.10587992742489405 1105 20-21 0.10053385951351146 1105 22-23 0.06498267858732021 1105 24-25 0.002770705156258657 1105 26-27 -0.024265247132937873 1105 28-29 0.1343128328726806 1105 30-31 0.09438984219639934 1105 32-33 0.2119127635478577 1105 34-35 0.2400164372362923 1105 36-37 0.21578734036135216 1105 38-39 0.35678445334404074 1105 40-41 0.2954954438795312 1105 42-43 0.3872135001654655 1105 44-45 0.3587951201926174 1105 46-47 0.3332810250170084 1105 48-49 0.366911274679687 1105 50-51 0.3783107891391637 1105 52-53 0.29510677116845585 1105 54-55 0.4231656130551045 1105 56-57 0.2630496092756758 1105 58-59 0.30416126566297663 1105 60-61 0.3326435481439205 1105 62-63 0.33691645470057097 1105 64-65 0.10108526599112366 1105 66-67 0.25238258262486113 1105 68-69 0.27527578350988335 1105 70-71 0.4334031246132426 1105 72-73 0.4158704018249999 1105 74-75 0.31695960570117165 1105 76-77 0.1653598576586326 1105 78-79 0.38678500471273836 1105 80-81 0.5743583157775767 1105 82-83 0.5244352447294567 1105 84-85 0.39856571055715406 1105 86-87 0.5100476376759211 1105 88-89 0.5720422595110257 1105 90-91 0.5106823486061991 1105 92-93 0.659190431139212 1105 94-95 0.8505559091764425 1105 96-97 0.8336912586574492 1105 98-99 0.7385116878357181 1105 100-101 0.8868185545774274 1106 1 0.017880003145158696 1106 2 0.059384251863185966 1106 3 0.024459513056115156 1106 4 0.0037583649789496576 1106 5 -0.0033540777110161457 1106 6 0.04279037938147212 1106 7 0.1042993953249578 1106 8 0.0652062757612697 1106 9 0.029649871524959792 1106 10-11 0.12313418815281096 1106 12-13 0.08871321601377957 1106 14-15 0.028687474175008276 1106 16-17 -0.08065751469213467 1106 18-19 0.00805092481246561 1106 20-21 0.08028287985312232 1106 22-23 0.04014303966214783 1106 24-25 0.07798762995565767 1106 26-27 0.010503366414368998 1106 28-29 0.05640417454090141 1106 30-31 0.07936838296893 1106 32-33 0.10982748644391904 1106 34-35 0.04419923719898122 1106 36-37 0.020050029295852312 1106 38-39 0.019666987398899494 1106 40-41 0.1482659001213662 1106 42-43 0.016252266726397124 1106 44-45 0.14514926742157286 1106 46-47 0.0306225299619598 1106 48-49 0.09809421459384993 1106 50-51 0.12655731367732415 1106 52-53 0.08375728898894153 1106 54-55 0.11443555614150824 1106 56-57 0.1995536212182074 1106 58-59 0.05483244965252965 1106 60-61 0.1691691359401517 1106 62-63 0.19345452150407283 1106 64-65 0.15280317045091607 1106 66-67 0.18087962031846416 1106 68-69 0.1654167846294925 1106 70-71 0.22580550845351155 1106 72-73 0.29365193755928587 1106 74-75 0.275645927776182 1106 76-77 0.13352556021508022 1106 78-79 0.3308603919118376 1106 80-81 0.3338645202933428 1106 82-83 0.2880035410533992 1106 84-85 0.4034873377263999 1106 86-87 0.5514009685212287 1106 88-89 0.5727291880424694 1106 90-91 0.848592289826577 1106 92-93 0.9369906270712889 1106 94-95 1.0006229692622775 1106 96-97 0.9932703545708641 1106 98-99 0.9566896361469688 1106 100-101 1.0011600828476261 1107 1 0.005106436447242402 1107 2 0.027479245545620756 1107 3 0.02560183097168789 1107 4 0.02192030837490222 1107 5 0.0015603388794147577 1107 6 -0.04853516797979296 1107 7 0.0020759698466434884 1107 8 -0.05510133403284101 1107 9 -0.0962970517789472 1107 10-11 0.029873956425056747 1107 12-13 0.07500600629440157 1107 14-15 0.05513296705364468 1107 16-17 0.07063983004889707 1107 18-19 -0.007597128760977512 1107 20-21 0.07014870698757392 1107 22-23 0.06705722326016428 1107 24-25 0.03901227958407105 1107 26-27 0.05436835301017595 1107 28-29 0.06375169553585636 1107 30-31 0.04947678140532474 1107 32-33 0.0799917475161891 1107 34-35 0.08830935715873522 1107 36-37 0.0792720069741577 1107 38-39 -0.06048962878796971 1107 40-41 -0.08630340856907992 1107 42-43 0.0437810461310022 1107 44-45 -0.11577317743783766 1107 46-47 -0.07416381201165478 1107 48-49 -0.06526038048357208 1107 50-51 -0.10008833050396504 1107 52-53 -0.11763737565579646 1107 54-55 -0.20491083891335649 1107 56-57 -0.003088535482575594 1107 58-59 0.034476553748291394 1107 60-61 0.08073477371841875 1107 62-63 0.17883382061278041 1107 64-65 0.08020203807362947 1107 66-67 0.08988875031683108 1107 68-69 0.0937806267711565 1107 70-71 0.1221757971931936 1107 72-73 0.22701350959153288 1107 74-75 0.02828485875688358 1107 76-77 0.04839560286009714 1107 78-79 0.25607552536693134 1107 80-81 0.15431641875422386 1107 82-83 0.11428452220778595 1107 84-85 0.37465057511319344 1107 86-87 0.3011909762800471 1107 88-89 0.4794912444027055 1107 90-91 0.5596042288361147 1107 92-93 0.7432681916682924 1107 94-95 0.7434257326433915 1107 96-97 0.8201927057206149 1107 98-99 1.0039857777585013 1107 100-101 0.8196346433806525 1108 1 0.06550107426514984 1108 2 0.05478019703710402 1108 3 0.11609383743275714 1108 4 0.024411691041173356 1108 5 -0.03724000385727777 1108 6 0.06372876613860257 1108 7 0.09831902260958003 1108 8 0.15939463773051443 1108 9 0.15919254682253836 1108 10-11 0.11494075883133092 1108 12-13 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-0.6634324710311148 2316 64-65 -0.6995483220462404 2316 66-67 -0.731313010807348 2316 68-69 -0.5690930603593571 2316 70-71 -0.5123609522491073 2316 72-73 -0.4633331546328243 2316 74-75 -0.701061057281219 2316 76-77 -0.563805164240275 2316 78-79 -0.6962090365286393 2316 80-81 -0.7628641884048335 2316 82-83 -0.7101440001811632 2316 84-85 -0.9523942463969242 2316 86-87 -1.0355537834034187 2316 88-89 -0.9966204503143459 2316 90-91 -1.1563629083535183 2316 92-93 -1.227975328555516 2316 94-95 -1.0304171236813175 2316 96-97 -0.8314006016218833 2316 98-99 -0.9668153037430756 2316 100-101 -0.5063536620055906 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 8.0 4 27.0 5 69.0 6 137.0 7 382.0 8 996.0 9 1937.0 10 3218.0 11 4932.0 12 7134.0 13 9494.0 14 12191.0 15 15882.0 16 20398.0 17 27249.0 18 28367.0 19 23413.0 20 23537.0 21 25480.0 22 28520.0 23 31971.0 24 36355.0 25 43004.0 26 51285.0 27 61551.0 28 74674.0 29 90799.0 30 109092.0 31 132515.0 32 163984.0 33 208629.0 34 279603.0 35 399939.0 36 611925.0 37 836494.0 38 462520.0 39 18700.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.6048228644449 19.522703877626444 15.034467674614294 22.838005583314366 2 15.527657709232548 21.394992842684122 38.676075950222724 24.401273497860604 3 20.772880922334412 23.736498463243947 29.81527729464379 25.67534331977785 4 13.399831635388182 17.032097948894737 37.398912337569485 32.16915807814759 5 13.888858557607165 37.333760744423245 34.27979931437438 14.497581383595215 6 31.723496222715497 36.31065194750227 16.963956557978417 15.001895271803814 7 28.044485071029797 32.40750995602657 20.960614224053884 18.587390748889746 8 25.54753596466735 34.250057326122466 19.68625842148037 20.51614828772982 9 24.932573560724354 18.107047239324732 21.168106189297355 35.79227301065356 10-11 25.14208038968244 25.99540767062516 27.238110614198057 21.624401325494343 12-13 26.6665002779212 23.487006338891238 27.248002945080795 22.598490438106765 14-15 22.843507044538455 25.272502693438824 26.574335791746222 25.3096544702765 16-17 22.123260514907088 28.18977855485431 26.9817943101532 22.705166620085407 18-19 21.97347441074106 28.08780368339322 28.083084984163108 21.85563692170261 20-21 23.227933862553538 26.231874675996913 27.77541627084344 22.764775190606105 22-23 22.514100146266106 26.275629716405984 28.086979196727135 23.12329094060078 24-25 23.34216841158379 26.320049084730957 27.856273772166773 22.48150873151848 26-27 22.869577074851676 25.8903651414493 28.95637715497827 22.283680628720756 28-29 22.122644624317612 27.68287553029913 28.006917612092717 22.18756223329054 30-31 22.704212287080903 26.35406641094796 27.70386911029338 23.237852191677753 32-33 22.706097159588126 26.490388190142816 28.088214113197385 22.715300537071673 34-35 23.589323964448578 26.272763722197173 27.84125627092801 22.29665604242624 36-37 23.088434992045276 25.907342945572427 28.65691036360809 22.34731169877421 38-39 22.51347507403815 26.829151921871876 28.32100447479063 22.336368529299346 40-41 22.782409921920156 26.856869562640025 27.816481292790712 22.54423922264911 42-43 22.381255585024583 26.907436204297046 27.215010674108083 23.496297536570292 44-45 22.959902151479316 26.096184347070544 27.55320071335097 23.39071278809917 46-47 23.632391292874548 26.84379831669729 27.374337914192797 22.149472476235367 48-49 23.167407791423095 26.63837276068506 27.522457659562384 22.671761788329466 50-51 22.03684207310724 27.07047035395912 28.331639754205924 22.561047818727715 52-53 23.029060314360336 27.53802632789918 26.63129865895871 22.801614698781776 54-55 22.597021537463206 26.598241894918228 27.220301818784975 23.584434748833587 56-57 23.17899217296942 26.488165340496366 27.181629434585048 23.151213051949167 58-59 23.979151852973636 26.40565102037368 26.949382342121236 22.66581478453145 60-61 22.96055914762927 26.814013112935896 28.072594452747403 22.152833286687436 62-63 22.397894737663112 26.916021803982805 27.88059572663672 22.805487731717363 64-65 22.245606363271087 27.722019095064937 27.18824538303678 22.844129158627194 66-67 22.85213285273343 26.68342127923326 26.690661996555654 23.773783871477658 68-69 22.414202137236284 26.7147905564341 27.33124952470834 23.539757781621276 70-71 23.367830399951746 26.59093638906264 26.878112964439083 23.163120246546523 72-73 22.950558624214658 26.028698418838946 27.92338526222086 23.097357694725527 74-75 22.209452752615814 27.79188615682035 26.88691336917971 23.111747721384127 76-77 22.607260597250985 26.53966125123507 26.895407565158358 23.95767058635559 78-79 22.7470864667763 26.701301908989866 27.2307362318087 23.32087539242514 80-81 23.316282353412205 26.4724100273853 26.931578864584388 23.27972875461811 82-83 22.83975411903139 26.267882240445257 27.773089428231074 23.119274212292282 84-85 22.510814632557278 26.85533441711477 27.302933723376793 23.330917226951165 86-87 22.44429287755011 26.977134766360443 27.101587609727336 23.476984746362113 88-89 22.32746708000965 26.89842876048285 26.5386642113982 24.2354399481093 90-91 22.6667574937925 26.096629882985688 26.90777092615872 24.328841697063087 92-93 23.112982637854376 26.712296180286366 27.003359492763913 23.17136168909535 94-95 22.762939130999968 26.425751519287644 27.143661239478728 23.66764811023366 96-97 21.734181725391718 26.45405908647107 27.985277713901535 23.826481474235674 98-99 22.327133792670264 26.92115305320748 26.645467700564733 24.10624545355752 100-101 22.415207996573415 25.376030428472358 27.727191482419588 24.48157009253464 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 537.0 1 391.5 2 277.0 3 295.5 4 270.5 5 314.5 6 533.0 7 1074.0 8 1748.5 9 2135.5 10 2054.5 11 1959.5 12 2091.0 13 2304.5 14 2619.5 15 2828.0 16 3070.5 17 3390.5 18 3728.5 19 4156.5 20 4754.0 21 5702.5 22 7154.5 23 9050.0 24 11252.5 25 14189.5 26 18558.0 27 23924.0 28 28523.0 29 33141.5 30 38168.0 31 44311.5 32 51205.0 33 56739.0 34 62361.0 35 69025.0 36 76613.5 37 84046.5 38 90761.5 39 96177.0 40 101205.5 41 106914.5 42 111242.5 43 116490.0 44 124717.0 45 131799.0 46 139456.5 47 172034.5 48 226375.0 49 222172.5 50 172718.5 51 149276.0 52 142625.0 53 138356.0 54 132900.5 55 123367.0 56 112393.0 57 101620.5 58 91534.5 59 81705.0 60 68615.5 61 56303.5 62 44360.0 63 32904.0 64 24300.5 65 17441.5 66 12169.5 67 9126.5 68 6794.5 69 4924.0 70 3534.0 71 2204.5 72 1382.5 73 898.5 74 564.5 75 350.0 76 210.5 77 101.0 78 51.0 79 32.0 80 21.5 81 16.5 82 14.0 83 8.0 84 6.0 85 4.0 86 1.5 87 1.0 88 0.5 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.38992162570123756 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 7.799472443683909E-4 16-17 0.002313843491626226 18-19 3.8997362218419544E-4 20-21 0.0 22-23 0.0 24-25 1.0399296591578547E-4 26-27 1.2999120739473184E-5 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.01279113480764161 36-37 0.023398417331051725 38-39 0.011023254387073259 40-41 0.0057976078498050395 42-43 0.004965664122478755 44-45 0.001572893609476255 46-47 0.001221917349510479 48-49 0.0031327880982130367 50-51 6.109586747552395E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 7.539490028894446E-4 60-61 0.0015468953679973088 62-63 0.0013649076776446841 64-65 7.14951640671025E-4 66-67 0.0028598065626841 68-69 0.0023788390953235925 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.09117583286666489 78-79 0.0036137555655735443 80-81 1.9498681109209772E-4 82-83 0.0 84-85 1.2999120739473182E-4 86-87 0.09341168163385428 88-89 0.4374464111247515 90-91 0.38892069340429813 92-93 0.0 94-95 0.0010139314176789082 96-97 1.2999120739473184E-5 98-99 0.0 100-101 4.28970984402615E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3846414.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.91473704983747 #Duplication Level Percentage of deduplicated Percentage of total 1 69.98677826238166 33.53398077407298 2 15.306739701697742 14.668368157943098 3 5.950952338099309 8.554149494284315 4 2.853039532899501 5.468105560466828 5 1.6451255804456881 3.941287980050819 6 1.0151886927307578 2.91854995608975 7 0.6835012749452646 2.292484870356171 8 0.49029941433400154 1.8794054010802395 9 0.3369894410923382 1.453208441265997 >10 1.6073945279128667 13.326520440610254 >50 0.08731644908766188 2.847033524988688 >100 0.03330783800755187 2.749718737519639 >500 0.0014119452780599574 0.42303932784136183 >1k 0.0011947228868519036 1.5473798759615296 >5k 4.887502718939605E-4 1.912133543500857 >10k+ 2.715279288299781E-4 2.48463391396742 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 38083 0.9900910302427143 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 18543 0.4820853917441024 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 14941 0.3884397259369376 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 12502 0.32503001496978745 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 11419 0.2968739194480885 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 9712 0.2524949212435271 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 9535 0.2478932325017536 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 8658 0.22509277472471761 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8566 0.22270093650865455 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 8473 0.22028310005111257 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 8149 0.2118596698119339 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7139 0.18560144591819808 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 6880 0.17886790137515096 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 6374 0.16571279118680413 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 4819 0.12528552568704254 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 4681 0.12169776836294793 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4543 0.11811001103885334 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 4533 0.11785002862406387 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 3862 0.10040520859169086 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.5998241478946364E-5 2 0.0 0.0 0.0 0.0 2.5998241478946364E-5 3 0.0 0.0 0.0 0.0 2.5998241478946364E-5 4 0.0 0.0 0.0 0.0 2.5998241478946364E-5 5 0.0 0.0 0.0 0.0 2.5998241478946364E-5 6 0.0 0.0 0.0 0.0 2.5998241478946364E-5 7 0.0 0.0 0.0 0.0 5.199648295789273E-5 8 0.0 0.0 0.0 0.0 1.0399296591578546E-4 9 0.0 0.0 0.0 5.199648295789273E-5 1.0399296591578546E-4 10-11 5.199648295789273E-5 0.0 0.0 1.42990328134205E-4 1.0399296591578546E-4 12-13 5.199648295789273E-5 0.0 0.0 1.8198769035262456E-4 1.0399296591578546E-4 14-15 5.199648295789273E-5 0.0 0.0 2.2098505257104407E-4 2.079859318315709E-4 16-17 5.199648295789273E-5 0.0 0.0 2.4698329404999046E-4 2.5998241478946365E-4 18-19 7.799472443683909E-5 0.0 0.0 3.639753807052491E-4 2.8598065626841E-4 20-21 7.799472443683909E-5 0.0 0.0 4.939665880999809E-4 3.509762599657759E-4 22-23 7.799472443683909E-5 0.0 0.0 7.019525199315519E-4 4.2897098440261496E-4 24-25 7.799472443683909E-5 0.0 0.0 9.229375725025959E-4 4.6796834662103453E-4 26-27 7.799472443683909E-5 0.0 0.0 0.0013259103154262646 4.939665880999809E-4 28-29 7.799472443683909E-5 0.0 0.0 0.003366772271523554 5.199648295789273E-4 30-31 7.799472443683909E-5 0.0 0.0 0.008839402102841764 5.329639503184005E-4 32-33 7.799472443683909E-5 0.0 0.0 0.02066860197576236 6.239577954947127E-4 34-35 7.799472443683909E-5 0.0 0.0 0.03832140793996694 6.499560369736591E-4 36-37 7.799472443683909E-5 0.0 0.0 0.06525558611215537 6.499560369736591E-4 38-39 7.799472443683909E-5 0.0 0.0 0.10565685337043802 6.759542784526055E-4 40-41 7.799472443683909E-5 0.0 0.0 0.14960688059059685 7.279507614104982E-4 42-43 7.799472443683909E-5 0.0 0.0 0.19802860534513445 7.279507614104982E-4 44-45 7.799472443683909E-5 0.0 0.0 0.25804554579928213 7.279507614104982E-4 46-47 7.799472443683909E-5 0.0 0.0 0.3304246500766688 7.279507614104982E-4 48-49 7.799472443683909E-5 0.0 0.0 0.4047276242234975 7.279507614104982E-4 50-51 7.799472443683909E-5 0.0 0.0 0.4884419617857048 7.279507614104982E-4 52-53 7.799472443683909E-5 0.0 0.0 0.574899113823941 8.319437273262836E-4 54-55 7.799472443683909E-5 0.0 0.0 0.6783461166686685 8.839402102841764E-4 56-57 7.799472443683909E-5 0.0 0.0 0.8092992589981214 8.969393310236496E-4 58-59 7.799472443683909E-5 0.0 0.0 0.9459070188492451 9.099384517631228E-4 60-61 9.099384517631228E-5 0.0 0.0 1.0942399856073735 9.229375725025959E-4 62-63 1.0399296591578546E-4 0.0 0.0 1.2487215364752728 9.879331761999618E-4 64-65 1.0399296591578546E-4 0.0 0.0 1.4136803786591874 0.001000932296939435 66-67 1.0399296591578546E-4 0.0 0.0 1.6119819655398508 0.0010139314176789082 68-69 1.0399296591578546E-4 0.0 0.0 1.8417284254892998 0.0010139314176789082 70-71 1.0399296591578546E-4 0.0 0.0 2.0883347450378453 0.0010399296591578546 72-73 1.0399296591578546E-4 0.0 0.0 2.3354480302952307 0.00114392262507364 74-75 1.0399296591578546E-4 0.0 0.0 2.609768475260333 0.0011959191080315327 76-77 1.0399296591578546E-4 0.0 0.0 2.8965680761353303 0.0012479155909894254 78-79 1.0399296591578546E-4 0.0 0.0 3.2452824890924377 0.0012479155909894254 80-81 1.0399296591578546E-4 0.0 0.0 3.634021194806383 0.0012999120739473182 82-83 1.0399296591578546E-4 0.0 0.0 4.003989170172529 0.001351908556905211 84-85 1.0399296591578546E-4 2.5998241478946364E-5 0.0 4.394963724653664 0.001351908556905211 86-87 1.0399296591578546E-4 2.5998241478946364E-5 0.0 4.812716467858114 0.0014039050398631037 88-89 1.0399296591578546E-4 2.5998241478946364E-5 0.0 5.273080328846557 0.0014559015228209965 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTAAGCG 830 0.0 26.587242 94-95 TTTGCGT 1010 0.0 23.493158 3 GAGTACT 9895 0.0 19.496147 12-13 GTACATG 10325 0.0 19.287752 1 TACATGG 10120 0.0 19.038744 2 GTACTTT 10110 0.0 18.870306 14-15 CTTTGCG 1355 0.0 18.211966 2 AGAGTAC 15935 0.0 17.97336 10-11 AGTACTT 10595 0.0 17.468987 12-13 ACATGGG 10600 0.0 17.146902 3 TTGCGTT 1465 0.0 16.844515 4 CGTATAC 175 0.0021844078 16.270689 3 ACTTTTT 12745 0.0 15.285124 16-17 CATGGGG 7080 0.0 15.081413 4 TACTTTT 12235 0.0 14.894684 14-15 TCGTATA 195 0.0044914354 14.601901 2 GTGTAGC 2205 0.0 14.260368 1 GTCTACG 270 1.6363178E-4 14.116324 1 GTATATA 2330 0.0 13.699802 1 CTACACT 1645 0.0 13.558909 4 >>END_MODULE