FastQCFastQC Report
Fri 27 May 2016
SRR938938_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938938_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3854126
Sequences flagged as poor quality0
Sequence length101
%GC46

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT354240.919118887135501No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT202110.5243990466321028No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT156610.40634374693510283No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC134940.3501182888156744No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT108690.2820094620674052No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT89770.23291921436922403No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC87820.2278597015250669No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87140.2260953585845403No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC82220.21332981848543614No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG74000.19200202588083526No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC73620.19101606953171743No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA71980.1867608894986827No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66140.1716082971859249No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA56580.1468037111396981No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT46300.12013099727409016No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA45390.11776989128015015No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC43300.11234713136000225No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42800.11104982037432093No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC39700.10300649226309676No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGCGT10150.029.849663
CTTTGCG12000.025.2478372
TTGCGTT13650.021.8490894
GAGTACT90550.019.29025512-13
GTACATG103350.019.1315861
TACATGG98500.018.9839382
AGAGTAC148200.018.06265610-11
GTACTTT96750.017.88307214-15
AGTACTT97300.017.63580312-13
ACATGGG103050.016.6757013
ATACCGA1800.002681945715.7780536
GTGTAGC23200.015.32846451
GTATATA22650.014.8633091
TACTTTT113900.014.774814-15
ACTTTTT118750.014.63102616-17
ATACGGT1950.00456890114.5643586
GAGTACA92100.014.4153041
GCACCGT6350.014.1629786
CATGGGG69350.014.1302334
AGTACAT82050.014.0778622
Produced by FastQC (version 0.11.5)