##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938938_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3854126 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.760543895036125 33.0 31.0 34.0 30.0 34.0 2 31.833067471068667 33.0 31.0 34.0 30.0 34.0 3 31.89429821443305 34.0 31.0 34.0 30.0 34.0 4 35.40982235661211 37.0 35.0 37.0 33.0 37.0 5 35.44440062416226 37.0 35.0 37.0 33.0 37.0 6 35.5225052320552 37.0 35.0 37.0 33.0 37.0 7 35.4363157820995 37.0 35.0 37.0 33.0 37.0 8 35.414344523245994 37.0 35.0 37.0 33.0 37.0 9 37.01712320770001 39.0 37.0 39.0 33.0 39.0 10-11 36.90094836027676 39.0 37.0 39.0 33.0 39.0 12-13 36.779203508136476 39.0 37.0 39.0 32.0 39.0 14-15 37.969174204475934 40.0 38.0 41.0 33.0 41.0 16-17 37.902183659797316 40.0 38.0 41.0 33.0 41.0 18-19 37.829052163836884 40.0 38.0 41.0 32.0 41.0 20-21 37.679672901197314 40.0 37.5 41.0 32.0 41.0 22-23 37.784713966279256 40.0 38.0 41.0 32.0 41.0 24-25 37.714957295116974 40.0 38.0 41.0 32.0 41.0 26-27 37.625203612959204 40.0 37.5 41.0 32.0 41.0 28-29 37.50734732076741 40.0 37.0 41.0 32.0 41.0 30-31 37.35231269034796 40.0 37.0 41.0 31.0 41.0 32-33 37.16931568921203 40.0 37.0 41.0 31.0 41.0 34-35 36.841064874371 40.0 36.0 41.0 30.0 41.0 36-37 36.571934077920645 39.5 36.0 41.0 30.0 41.0 38-39 36.36636905487781 39.0 35.5 41.0 30.0 41.0 40-41 36.25505224790264 39.0 35.0 41.0 30.0 41.0 42-43 36.053867725134054 39.0 35.0 41.0 29.0 41.0 44-45 36.008502965393454 39.0 35.0 41.0 28.5 41.0 46-47 35.81904898801959 39.0 35.0 41.0 27.0 41.0 48-49 35.64873696397056 39.0 35.0 41.0 27.0 41.0 50-51 34.491602376258584 38.0 34.0 40.0 25.0 40.5 52-53 34.6621970843714 38.0 34.0 39.5 25.5 40.5 54-55 35.16520567827829 39.0 35.0 40.0 26.0 41.0 56-57 35.03372775046794 39.0 34.5 40.5 26.0 41.0 58-59 34.81851462562459 38.0 34.0 40.0 26.0 41.0 60-61 34.494062077887435 38.0 34.0 40.0 25.0 41.0 62-63 34.1508719486597 37.5 34.0 40.0 23.5 41.0 64-65 33.739775113735256 37.0 33.5 40.0 22.5 41.0 66-67 33.308261847173654 36.0 33.0 39.0 22.0 41.0 68-69 32.891769236397565 36.0 32.5 39.0 21.5 40.5 70-71 32.434603461329495 35.0 32.0 38.5 20.0 40.0 72-73 31.983617556872815 35.0 31.5 37.5 18.5 40.0 74-75 31.521164331420405 35.0 31.0 37.0 17.5 39.0 76-77 31.10560293046984 35.0 31.0 36.5 14.0 39.0 78-79 30.664820117453345 35.0 30.5 36.0 9.5 38.5 80-81 30.201614192167042 34.0 30.0 35.5 7.0 37.0 82-83 29.793211093773273 34.0 30.0 35.0 4.0 37.0 84-85 29.41839044701704 34.0 29.0 35.0 2.0 36.5 86-87 29.050129134335513 34.0 29.0 35.0 2.0 36.0 88-89 28.609159119343786 34.0 29.0 35.0 2.0 36.0 90-91 28.15446121896378 34.0 28.5 35.0 2.0 35.0 92-93 27.709449431596163 34.0 27.0 35.0 2.0 35.0 94-95 27.195677437634366 33.5 26.0 35.0 2.0 35.0 96-97 26.564219488413197 33.0 25.0 35.0 2.0 35.0 98-99 25.812690866878768 33.0 23.5 35.0 2.0 35.0 100-101 23.043245991438788 29.5 9.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3493470564318848 1101 2 0.4047899692714516 1101 3 0.351826843382117 1101 4 0.3095327193633324 1101 5 0.05782624188492491 1101 6 0.24770671967380764 1101 7 0.2624698748147978 1101 8 0.3186919596509057 1101 9 0.3966850069810377 1101 10-11 0.4487977522752473 1101 12-13 0.551166782217031 1101 14-15 0.26206989377164547 1101 16-17 0.48595270961029513 1101 18-19 0.553533410756458 1101 20-21 0.6170045200867094 1101 22-23 0.6216252302037546 1101 24-25 0.657935370124811 1101 26-27 0.6252422857655375 1101 28-29 0.5935409233036495 1101 30-31 0.590664570439408 1101 32-33 0.6097216459805139 1101 34-35 0.555409989883259 1101 36-37 0.5868953650828743 1101 38-39 0.5385481337801892 1101 40-41 0.4748154435344034 1101 42-43 0.4688150557425317 1101 44-45 0.6783024664173851 1101 46-47 0.7063400853367057 1101 48-49 0.7519453587321294 1101 50-51 0.6712032049061349 1101 52-53 0.7297331144976695 1101 54-55 0.6594518077287219 1101 56-57 0.6303943822077471 1101 58-59 0.47836307097428943 1101 60-61 0.5539753796642302 1101 62-63 0.6138608098041871 1101 64-65 0.6656382745981517 1101 66-67 0.5522990499713316 1101 68-69 0.540202315094696 1101 70-71 0.5283141579075092 1101 72-73 0.496333698722232 1101 74-75 0.5760026471328388 1101 76-77 0.6320670402787307 1101 78-79 0.6754858297662203 1101 80-81 0.5157139781631876 1101 82-83 0.5136708153230352 1101 84-85 0.2956487569929429 1101 86-87 0.3124647730883403 1101 88-89 0.33629106701619094 1101 90-91 0.380617495281502 1101 92-93 0.5719961203818116 1101 94-95 0.5701852617356558 1101 96-97 0.41426270671936294 1101 98-99 0.3905547933809963 1101 100-101 0.7210377480160481 1102 1 0.3280855427446703 1102 2 0.26872163548548755 1102 3 0.23080601196798156 1102 4 0.2631672101170892 1102 5 0.3425750254628994 1102 6 0.3321121115309893 1102 7 0.3627010159365014 1102 8 0.3141841394959215 1102 9 0.517008795972643 1102 10-11 0.3369972921464992 1102 12-13 0.4147393840979916 1102 14-15 0.5641891061211837 1102 16-17 0.5925879373357432 1102 18-19 0.5670385516125691 1102 20-21 0.6013328050841906 1102 22-23 0.5968444074992831 1102 24-25 0.5989500431594266 1102 26-27 0.6096768244942368 1102 28-29 0.5669514691771553 1102 30-31 0.5654958954451246 1102 32-33 0.6004691219122833 1102 34-35 0.5266654020191268 1102 36-37 0.5794893828108698 1102 38-39 0.6017118270372563 1102 40-41 0.5640039730964261 1102 42-43 0.5190504888868759 1102 44-45 0.6505435482822577 1102 46-47 0.6481319584292251 1102 48-49 0.652915236210589 1102 50-51 0.5933750012626717 1102 52-53 0.5554725836387746 1102 54-55 0.6758029932840728 1102 56-57 0.6700456426566532 1102 58-59 0.5861928867433335 1102 60-61 0.553488751034557 1102 62-63 0.5425209629650922 1102 64-65 0.58247854136755 1102 66-67 0.602322349522808 1102 68-69 0.5170927641329826 1102 70-71 0.5363986386714785 1102 72-73 0.45889036239395864 1102 74-75 0.38873025892582547 1102 76-77 0.3559850019298807 1102 78-79 0.38126363997926305 1102 80-81 0.28009330084313433 1102 82-83 0.30860163748913294 1102 84-85 0.3503750366138796 1102 86-87 0.40268794929277263 1102 88-89 0.39141305018787165 1102 90-91 0.33554437835783446 1102 92-93 0.588738971696575 1102 94-95 0.616456256755118 1102 96-97 0.5009953618632714 1102 98-99 0.5552332715344512 1102 100-101 0.9378522626496029 1103 1 -0.029786482665112857 1103 2 0.18440518893438806 1103 3 0.2546647460772071 1103 4 0.22873005103677713 1103 5 0.22806262268498045 1103 6 0.23227733931182826 1103 7 0.26978370884631886 1103 8 0.2223051502788067 1103 9 0.36076397605072685 1103 10-11 0.4095718570496416 1103 12-13 0.4373959687607325 1103 14-15 0.3745195260350229 1103 16-17 0.44882810818399577 1103 18-19 0.47861216798585815 1103 20-21 0.49215993024557037 1103 22-23 0.5232634592546148 1103 24-25 0.48087304113165885 1103 26-27 0.5146236542418947 1103 28-29 0.49291318873433454 1103 30-31 0.4993554331162784 1103 32-33 0.6320106359120885 1103 34-35 0.5525937165709891 1103 36-37 0.5717455931117925 1103 38-39 0.616994839767699 1103 40-41 0.5169914410950511 1103 42-43 0.4689482909593252 1103 44-45 0.6138394087945969 1103 46-47 0.5407444838357449 1103 48-49 0.4755882040306787 1103 50-51 0.4251399158771676 1103 52-53 0.4541985666430719 1103 54-55 0.6633211588338739 1103 56-57 0.7238473817963964 1103 58-59 0.5291171897239124 1103 60-61 0.5074749529404059 1103 62-63 0.7083438400911106 1103 64-65 0.47609165734009906 1103 66-67 0.45046228931359167 1103 68-69 0.3596040199413508 1103 70-71 0.3695547848656773 1103 72-73 0.41994501292323605 1103 74-75 0.362819953964415 1103 76-77 0.4084583388240368 1103 78-79 0.3590872615511991 1103 80-81 0.3728589560416893 1103 82-83 0.3246703882280819 1103 84-85 0.32612907774318245 1103 86-87 0.44993445743741134 1103 88-89 0.3875197851954262 1103 90-91 0.3618397172233152 1103 92-93 0.4859525947003682 1103 94-95 0.5673792686894217 1103 96-97 0.5002843358052864 1103 98-99 0.5131297454274524 1103 100-101 0.8543738004859307 1104 1 0.20473025368098163 1104 2 0.2347449191379276 1104 3 0.1722815018008852 1104 4 0.2065793826260105 1104 5 0.288049594753538 1104 6 0.21338140981293208 1104 7 0.23922409628794128 1104 8 0.24520421175090945 1104 9 0.33349507796776123 1104 10-11 0.30664244001953733 1104 12-13 0.3957291362107682 1104 14-15 0.35525182491768703 1104 16-17 0.31242158344732474 1104 18-19 0.39926042592529143 1104 20-21 0.404728923522633 1104 22-23 0.45396033632234634 1104 24-25 0.4234623030556932 1104 26-27 0.42861223457015285 1104 28-29 0.38388044858278647 1104 30-31 0.48170040721061724 1104 32-33 0.6049805373947805 1104 34-35 0.544531534872398 1104 36-37 0.4723383430006294 1104 38-39 0.46707905660755245 1104 40-41 0.5431554116333146 1104 42-43 0.4960145821964943 1104 44-45 0.6108876753334229 1104 46-47 0.6982394206551987 1104 48-49 0.7668792294739433 1104 50-51 0.6664327256215188 1104 52-53 0.6246910692125738 1104 54-55 0.6208575797488365 1104 56-57 0.6644858561197893 1104 58-59 0.7311623245066841 1104 60-61 0.6818689583257438 1104 62-63 0.6308720926711331 1104 64-65 0.6798977489078268 1104 66-67 0.7723307703261284 1104 68-69 0.6424326807824841 1104 70-71 0.6155858007236645 1104 72-73 0.5901798595200347 1104 74-75 0.7507707315758552 1104 76-77 0.7059275701261392 1104 78-79 0.7208601706199609 1104 80-81 0.7117837267951934 1104 82-83 0.5928045152629373 1104 84-85 0.7312828703819143 1104 86-87 0.7204090952803917 1104 88-89 0.8926206785548061 1104 90-91 0.947029164034241 1104 92-93 1.1202228079543914 1104 94-95 1.1340337721893654 1104 96-97 1.1311653180004235 1104 98-99 1.132823521720109 1104 100-101 1.6970882023581666 1105 1 0.2287309664988122 1105 2 0.20678921937218675 1105 3 0.30036599090911764 1105 4 0.31868442170903677 1105 5 0.2761802961317201 1105 6 0.3090115677244114 1105 7 0.31401699049543197 1105 8 0.3445300143696315 1105 9 0.3251668284866369 1105 10-11 0.33811035336836426 1105 12-13 0.43438225895943816 1105 14-15 0.47614651795909424 1105 16-17 0.4445931839573021 1105 18-19 0.4163493160293896 1105 20-21 0.4460145086807117 1105 22-23 0.45331109183173623 1105 24-25 0.4326329348189404 1105 26-27 0.4364008217906772 1105 28-29 0.551890487196836 1105 30-31 0.5849987256673899 1105 32-33 0.5029968856981668 1105 34-35 0.5179612574273946 1105 36-37 0.5558791844968667 1105 38-39 0.5523774093398757 1105 40-41 0.4739929533817602 1105 42-43 0.371638786685331 1105 44-45 0.3514527768847202 1105 46-47 0.508502127162771 1105 48-49 0.580556407335429 1105 50-51 0.539185605461725 1105 52-53 0.5108456211168928 1105 54-55 0.5127494831722572 1105 56-57 0.5508644080400487 1105 58-59 0.5891861721883842 1105 60-61 0.7412118958790117 1105 62-63 0.6105241445575018 1105 64-65 0.5355916764238415 1105 66-67 0.46513558403669464 1105 68-69 0.4983185016454783 1105 70-71 0.49281476977105143 1105 72-73 0.5930592552803446 1105 74-75 0.5350283162341753 1105 76-77 0.5286247664068249 1105 78-79 0.5210507341890676 1105 80-81 0.5122539703841866 1105 82-83 0.5671777756628167 1105 84-85 0.6788557022093187 1105 86-87 0.7477955505612037 1105 88-89 0.6181844634837859 1105 90-91 0.7365318335099893 1105 92-93 0.7539406841298835 1105 94-95 0.8092256779807911 1105 96-97 0.6223705440416865 1105 98-99 0.7456666941889551 1105 100-101 0.9749292176713062 1106 1 0.1690118874221902 1106 2 0.2026391949500912 1106 3 0.25839964483446565 1106 4 0.19833668272013227 1106 5 0.20733875612780395 1106 6 0.23943704667711785 1106 7 0.2791434802733974 1106 8 0.2475639323463099 1106 9 0.1785649640933613 1106 10-11 0.31400918303771874 1106 12-13 0.3527882061730665 1106 14-15 0.3092469888895124 1106 16-17 0.39296103920373326 1106 18-19 0.3267110649385643 1106 20-21 0.4526811508392683 1106 22-23 0.4099036688292088 1106 24-25 0.3511888646023493 1106 26-27 0.4381024152255719 1106 28-29 0.3735263583615591 1106 30-31 0.28771170993027795 1106 32-33 0.3935317731002499 1106 34-35 0.3810625901478417 1106 36-37 0.342563425835678 1106 38-39 0.34897177637207477 1106 40-41 0.5168343450644741 1106 42-43 0.5373963556089123 1106 44-45 0.6434442025335514 1106 46-47 0.6294814684511962 1106 48-49 0.5404723548834554 1106 50-51 0.4965530511634739 1106 52-53 0.4364780912156405 1106 54-55 0.40134255052047507 1106 56-57 0.4768725238538778 1106 58-59 0.6431566381707583 1106 60-61 0.6106409165129847 1106 62-63 0.6615185364182992 1106 64-65 0.4829823296571405 1106 66-67 0.577107416864699 1106 68-69 0.5363167606741044 1106 70-71 0.6318737799058951 1106 72-73 0.5972714572514093 1106 74-75 0.6048660049062775 1106 76-77 0.5403538416820979 1106 78-79 0.6606803002180541 1106 80-81 0.6855201764727532 1106 82-83 0.7610581898904236 1106 84-85 0.6989595171536607 1106 86-87 0.7583391166624445 1106 88-89 0.8679652513917517 1106 90-91 0.8141639144953423 1106 92-93 0.8526925944369843 1106 94-95 0.808234937592875 1106 96-97 0.8782333496157051 1106 98-99 0.9361525465166061 1106 100-101 1.402260311953043 1107 1 0.15408938711475528 1107 2 0.0832110726401254 1107 3 0.12218227754022948 1107 4 0.13890866734316631 1107 5 0.07700270389561581 1107 6 0.10830510739367583 1107 7 0.03824099629616029 1107 8 0.15133721605254635 1107 9 0.11299364780158783 1107 10-11 0.19291023258512752 1107 12-13 0.1899265721298775 1107 14-15 0.06923434024309216 1107 16-17 0.1551321152017735 1107 18-19 0.17377281340006334 1107 20-21 0.20293989051164374 1107 22-23 0.12210877243582985 1107 24-25 0.1543863140975077 1107 26-27 0.2410092360998064 1107 28-29 0.2634460898680686 1107 30-31 0.35365270888310363 1107 32-33 0.36581664872571196 1107 34-35 0.20793080515344542 1107 36-37 0.2245200858903189 1107 38-39 0.24744156512593207 1107 40-41 0.23219702743298143 1107 42-43 0.2579743132038672 1107 44-45 0.3733994205203004 1107 46-47 0.44142221840289153 1107 48-49 0.2763894695866682 1107 50-51 0.19489276355952256 1107 52-53 0.12498378654366604 1107 54-55 0.1666551653302406 1107 56-57 0.2600857230524909 1107 58-59 0.24108206156815726 1107 60-61 0.23341986079401522 1107 62-63 0.14695464356822185 1107 64-65 0.14626253001903677 1107 66-67 0.06923065562088482 1107 68-69 0.10807949773120384 1107 70-71 0.2367185322834331 1107 72-73 0.18784027207476583 1107 74-75 0.2393754994110644 1107 76-77 0.27453405892412874 1107 78-79 0.2832976992866669 1107 80-81 0.2761596434575715 1107 82-83 0.410583751078633 1107 84-85 0.41968527143231427 1107 86-87 0.4581166960200953 1107 88-89 0.5991769321417486 1107 90-91 0.5358681057343198 1107 92-93 0.5686968748122574 1107 94-95 0.5769949959782927 1107 96-97 0.6762434028454258 1107 98-99 0.7599865376151946 1107 100-101 0.8723156345049858 1108 1 0.18626786306928977 1108 2 0.1371647556892448 1108 3 0.09867257996573287 1108 4 0.19756064414936247 1108 5 0.175399375912221 1108 6 0.1825360573905499 1108 7 0.124895360649802 1108 8 0.13018461395648728 1108 9 0.19938341209627453 1108 10-11 0.2592244124069012 1108 12-13 0.18654007997348288 1108 14-15 0.3012565149291575 1108 16-17 0.32145437786648046 1108 18-19 0.22131391931949906 1108 20-21 0.318162759361293 1108 22-23 0.34208563883067455 1108 24-25 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-2.220692722332906 2316 64-65 -2.5806733868264757 2316 66-67 -2.659456293193287 2316 68-69 -2.857851244665497 2316 70-71 -2.6557133685106464 2316 72-73 -2.649037562872323 2316 74-75 -2.7153983654918683 2316 76-77 -2.6946489137754845 2316 78-79 -2.503772842458087 2316 80-81 -2.5401582640513816 2316 82-83 -2.719066355176256 2316 84-85 -3.0027011914756905 2316 86-87 -2.9840771125786993 2316 88-89 -3.1528483520033603 2316 90-91 -3.454165534801003 2316 92-93 -3.850138173600353 2316 94-95 -4.101390497947193 2316 96-97 -4.307464694251827 2316 98-99 -4.883536894828513 2316 100-101 -5.218138033479523 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 12943.0 3 3567.0 4 3587.0 5 4022.0 6 4627.0 7 5695.0 8 6918.0 9 8627.0 10 10677.0 11 13141.0 12 16577.0 13 23100.0 14 21579.0 15 19600.0 16 23436.0 17 28614.0 18 26808.0 19 22194.0 20 22942.0 21 24970.0 22 27343.0 23 30954.0 24 34848.0 25 40629.0 26 47000.0 27 55000.0 28 64885.0 29 76688.0 30 92204.0 31 112007.0 32 139179.0 33 179151.0 34 239912.0 35 341417.0 36 522104.0 37 783445.0 38 667662.0 39 96058.0 40 16.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.62628573331682 18.701168673432324 14.542199320797684 23.130346272453174 2 15.052100037265323 20.730036758929717 39.582498542497405 24.635364661307555 3 20.726751818476693 23.479768026545383 29.63478801946599 26.15869213551193 4 12.932136122553933 16.37657594946572 37.92894136714752 32.76234656083282 5 13.970398776511853 37.040516616207476 34.326733465351474 14.662351141929197 6 31.63377338196564 35.87949221722084 17.349201675020474 15.13753272579304 7 28.076909492457187 32.26659450385295 21.109882427246795 18.546613576443065 8 25.49595721280759 33.78466428700348 20.046121664067563 20.673256836121364 9 25.04770183257624 18.12235925849264 21.35943535366101 35.470503555270106 10-11 25.10339164590567 25.726435787331393 27.409280817955782 21.760891748807154 12-13 26.72175528721816 23.33023034041464 27.352581758063423 22.595432614303782 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26.739553326011006 28.288108910688294 22.412784732130895 40-41 22.79353520283673 26.80746845001102 27.850192814544624 22.548803532607632 42-43 22.39544008953892 26.78490830870056 27.203487113126375 23.61616448863414 44-45 22.921963458821043 26.015933759816708 27.565443625778453 23.496659155583796 46-47 23.621539473514268 26.721683802499292 27.4087826594393 22.247994064547136 48-49 23.143567004982085 26.48081936879686 27.55132799022896 22.824285635992098 50-51 22.02062614332351 26.94244303932902 28.39980375936514 22.637127057982322 52-53 22.895695210855205 27.420192881067983 26.790099937498287 22.894011970578525 54-55 22.52976478597562 26.45909228851522 27.40269086660037 23.608452058908796 56-57 23.139419297455913 26.35210108575043 27.26450396579797 23.24397565099569 58-59 23.940389728366593 26.314862070382222 26.992298948694653 22.752449252556527 60-61 22.93529653311433 26.627806782555417 28.197779993873144 22.239116690457102 62-63 22.414002282453342 26.75095063085779 28.002060156786907 22.832986929901967 64-65 22.23194448522575 27.553219602431216 27.371485059361266 22.843350852981768 66-67 22.82174443169149 26.60415080342299 26.788858345029826 23.785246419855696 68-69 22.365702971485927 26.588276292785306 27.464523004494463 23.581497731234307 70-71 23.36095731343307 26.478208788320245 26.9882230901774 23.172610808069287 72-73 22.940391377219814 25.903706989490427 28.107923216471413 23.047978416818342 74-75 22.23143554536609 27.676365973031324 27.0755946638938 23.016603817708788 76-77 22.635826453931813 26.491000922095758 26.958820667314455 23.914351956657974 78-79 22.771779711560587 26.612368383016317 27.368101245479277 23.247750659943822 80-81 23.358310165531137 26.434422086332276 27.00430240859485 23.202965339541734 82-83 22.92249932552068 26.149158818614897 27.887384653599423 23.040957202264998 84-85 22.669051013825943 26.795749508900336 27.389439948061383 23.14575952921234 86-87 22.541558275517474 27.001372447209054 27.21682839452378 23.240240882749696 88-89 22.488589971074198 26.885262714130093 26.659365083217306 23.96678223157841 90-91 22.792077703998835 26.127267545437032 26.999939450344847 24.080715300219282 92-93 23.337941950220152 26.638397234934274 27.125551618031707 22.898109196813866 94-95 23.05364109228527 26.376478826901185 27.21821914481251 23.351660936001036 96-97 22.09644859442316 26.376047806042624 28.09273167487015 23.434771924664062 98-99 22.75946286463321 26.86981903642528 26.7359449866139 23.634773112327608 100-101 22.782723810689408 25.68747523377296 27.451189411712974 24.078611543824657 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3566.0 1 2010.5 2 487.0 3 497.5 4 440.5 5 451.5 6 703.0 7 1385.5 8 2291.0 9 2779.0 10 2748.5 11 2707.5 12 2987.0 13 3365.0 14 3715.5 15 4156.5 16 4720.5 17 5028.5 18 5184.5 19 5514.5 20 5866.5 21 6488.0 22 7753.5 23 9452.5 24 11333.5 25 13995.5 26 18384.5 27 23773.0 28 28198.0 29 32663.5 30 37844.0 31 44295.0 32 50924.5 33 56457.0 34 62358.5 35 68772.5 36 76458.5 37 84041.5 38 90215.0 39 95815.0 40 101261.5 41 106260.0 42 110655.5 43 116632.0 44 124228.5 45 131011.5 46 139388.5 47 169312.5 48 222336.5 49 220254.0 50 170820.5 51 147889.5 52 141885.5 53 137705.0 54 132363.0 55 122917.5 56 111798.0 57 101039.0 58 91770.0 59 82215.5 60 69010.5 61 56766.0 62 44998.5 63 33503.5 64 24886.0 65 18329.0 66 13196.0 67 9751.0 68 7295.0 69 5365.0 70 3884.5 71 2560.0 72 1593.5 73 1038.0 74 695.5 75 441.5 76 272.5 77 172.0 78 116.0 79 78.5 80 70.5 81 54.5 82 38.5 83 29.5 84 30.0 85 31.0 86 21.0 87 15.5 88 17.0 89 14.5 90 13.0 91 12.0 92 9.0 93 8.0 94 6.5 95 5.0 96 4.0 97 1.5 98 0.5 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.28006349558888316 2 0.08720524445749828 3 0.09114906985396949 4 0.10549732935560487 5 0.10767681181154949 6 0.08271654844704092 7 0.09273178925650069 8 0.08928094203458839 9 0.09457397085616817 10-11 0.08706254024907334 12-13 0.09191448333552146 14-15 0.10056754761001585 16-17 0.08921607648530433 18-19 0.1002043005340251 20-21 0.10225405189140159 22-23 0.1139817432019607 24-25 0.12590403116037205 26-27 0.12847270691202103 28-29 0.12119479228234883 30-31 0.11469526424408544 32-33 0.10918169255493981 34-35 0.37767317415154567 36-37 0.5763304053889261 38-39 0.5592577928173599 40-41 0.5223233490550128 42-43 0.5489182242614797 44-45 0.5711022421166304 46-47 0.5594653625750688 48-49 0.5810915367063765 50-51 0.5733076707922885 52-53 0.5768104104536281 54-55 0.5663800301287504 56-57 0.5393310960772948 58-59 0.533415357982588 60-61 0.48132832190748304 62-63 0.5554566716293137 64-65 0.589329461465453 66-67 0.6020820284547002 68-69 0.6045079999979243 70-71 0.600784717469019 72-73 0.591820298557961 74-75 0.5894462194541642 76-77 0.5732687514627182 78-79 0.5506825672020064 80-81 0.545921435884556 82-83 0.5588815726315123 84-85 0.568481673925554 86-87 0.5592967121469303 88-89 0.5729055043867274 90-91 0.5853467167394112 92-93 0.5940905927829033 94-95 0.5925467927099425 96-97 0.5896927085414436 98-99 0.5945057322983214 100-101 0.5719195480376096 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3854126.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.82622894296287 #Duplication Level Percentage of deduplicated Percentage of total 1 71.74385916696824 35.74725952105045 2 14.613846559761795 14.56305728848043 3 5.567044495624899 8.321545007240024 4 2.6890574359091617 5.359423657695464 5 1.4870793428282447 3.704777789605544 6 0.9052529996552119 2.7063205927274683 7 0.6341635616018779 2.2118585165361937 8 0.43740801271641166 1.7435513426475475 9 0.321737042045318 1.4427849164743516 >10 1.4887642359988118 12.815076305000831 >50 0.07789666774829782 2.6314568383551142 >100 0.03086357190460693 2.643845583149878 >500 0.001252513364610709 0.39525077629642624 >1k 0.0010437611014829642 1.4340061800677675 >5k 4.696924956673339E-4 1.792332202541045 >10k+ 2.6094027537074105E-4 2.487453482131441 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 35424 0.919118887135501 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 20211 0.5243990466321028 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 15661 0.40634374693510283 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 13494 0.3501182888156744 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 10869 0.2820094620674052 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 8977 0.23291921436922403 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 8782 0.2278597015250669 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8714 0.2260953585845403 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 8222 0.21332981848543614 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 7400 0.19200202588083526 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 7362 0.19101606953171743 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 7198 0.1867608894986827 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6614 0.1716082971859249 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 5658 0.1468037111396981 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 4630 0.12013099727409016 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 4539 0.11776989128015015 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 4330 0.11234713136000225 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4280 0.11104982037432093 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 3970 0.10300649226309676 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.5946219713626384E-5 2 0.0 0.0 0.0 0.0 5.189243942725277E-5 3 0.0 0.0 0.0 0.0 5.189243942725277E-5 4 0.0 0.0 0.0 0.0 5.189243942725277E-5 5 0.0 0.0 0.0 0.0 5.189243942725277E-5 6 0.0 0.0 0.0 0.0 5.189243942725277E-5 7 0.0 0.0 0.0 0.0 5.189243942725277E-5 8 0.0 0.0 0.0 2.5946219713626384E-5 5.189243942725277E-5 9 0.0 0.0 0.0 5.189243942725277E-5 5.189243942725277E-5 10-11 0.0 0.0 0.0 5.189243942725277E-5 5.189243942725277E-5 12-13 0.0 0.0 0.0 6.486554928406595E-5 1.0378487885450554E-4 14-15 0.0 0.0 0.0 7.783865914087915E-5 1.8162353799538468E-4 16-17 0.0 0.0 0.0 1.297310985681319E-4 2.72435306993077E-4 18-19 0.0 0.0 0.0 2.2054286756582425E-4 3.113546365635166E-4 20-21 0.0 0.0 0.0 3.891932957043958E-4 3.243277464203298E-4 22-23 0.0 0.0 0.0 7.524403716951652E-4 3.6324707599076937E-4 24-25 0.0 0.0 0.0 0.0010378487885450553 4.1513951541802214E-4 26-27 0.0 0.0 0.0 0.0017124505010993414 4.410857351316485E-4 28-29 1.2973109856813192E-5 0.0 0.0 0.0036973363091917597 4.410857351316485E-4 30-31 2.5946219713626384E-5 0.0 0.0 0.009626047513755388 4.410857351316485E-4 32-33 2.5946219713626384E-5 0.0 0.0 0.021639147241164405 4.410857351316485E-4 34-35 2.5946219713626384E-5 0.0 0.0 0.03925663042671672 4.540588449884617E-4 36-37 2.5946219713626384E-5 0.0 0.0 0.06704503174001057 4.929781745589013E-4 38-39 2.5946219713626384E-5 0.0 0.0 0.10720977985670421 4.929781745589013E-4 40-41 2.5946219713626384E-5 0.0 0.0 0.15149997690786443 4.929781745589013E-4 42-43 2.5946219713626384E-5 0.0 0.0 0.20232862132685853 4.929781745589013E-4 44-45 2.5946219713626384E-5 0.0 0.0 0.2657411823069614 5.318975041293408E-4 46-47 2.5946219713626384E-5 0.0 0.0 0.342438207780441 6.227092731270332E-4 48-49 2.5946219713626384E-5 0.0 0.0 0.4227158115744011 7.524403716951652E-4 50-51 2.5946219713626384E-5 0.0 0.0 0.5099340291417561 7.524403716951652E-4 52-53 2.5946219713626384E-5 0.0 0.0 0.5993447022749127 8.04332811122418E-4 54-55 2.5946219713626384E-5 0.0 0.0 0.7033890433265544 8.302790308360443E-4 56-57 2.5946219713626384E-5 0.0 0.0 0.8414488784227605 8.432521406928575E-4 58-59 5.189243942725277E-5 0.0 0.0 0.9773811235024491 8.562252505496707E-4 60-61 5.189243942725277E-5 0.0 0.0 1.1279081171710525 8.691983604064838E-4 62-63 6.486554928406595E-5 0.0 0.0 1.2825475866642657 8.82171470263297E-4 64-65 7.783865914087915E-5 0.0 0.0 1.452196944261812 9.210907998337366E-4 66-67 7.783865914087915E-5 0.0 0.0 1.6510098528174741 9.340639096905498E-4 68-69 7.783865914087915E-5 0.0 0.0 1.8790252316608227 9.340639096905498E-4 70-71 7.783865914087915E-5 0.0 0.0 2.1255921575994146 9.340639096905498E-4 72-73 7.783865914087915E-5 0.0 0.0 2.3782175258411375 9.600101294041763E-4 74-75 7.783865914087915E-5 0.0 0.0 2.6584366987483024 9.600101294041763E-4 76-77 7.783865914087915E-5 0.0 0.0 2.949371660397195 9.859563491178027E-4 78-79 7.783865914087915E-5 0.0 0.0 3.309959248867318 0.001024875678688242 80-81 7.783865914087915E-5 0.0 0.0 3.6935351880037137 0.001076768118115495 82-83 9.081176899769234E-5 0.0 0.0 4.0712862008144 0.001141633667399561 84-85 1.0378487885450554E-4 0.0 0.0 4.462191947019895 0.001141633667399561 86-87 1.0378487885450554E-4 0.0 0.0 4.884323968650739 0.0011546067772563741 88-89 1.0378487885450554E-4 0.0 0.0 5.344168820635339 0.0011805529969700006 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTTGCGT 1015 0.0 29.84966 3 CTTTGCG 1200 0.0 25.247837 2 TTGCGTT 1365 0.0 21.849089 4 GAGTACT 9055 0.0 19.290255 12-13 GTACATG 10335 0.0 19.131586 1 TACATGG 9850 0.0 18.983938 2 AGAGTAC 14820 0.0 18.062656 10-11 GTACTTT 9675 0.0 17.883072 14-15 AGTACTT 9730 0.0 17.635803 12-13 ACATGGG 10305 0.0 16.675701 3 ATACCGA 180 0.0026819457 15.778053 6 GTGTAGC 2320 0.0 15.3284645 1 GTATATA 2265 0.0 14.863309 1 TACTTTT 11390 0.0 14.7748 14-15 ACTTTTT 11875 0.0 14.631026 16-17 ATACGGT 195 0.004568901 14.564358 6 GAGTACA 9210 0.0 14.415304 1 GCACCGT 635 0.0 14.162978 6 CATGGGG 6935 0.0 14.130233 4 AGTACAT 8205 0.0 14.077862 2 >>END_MODULE