FastQCFastQC Report
Fri 27 May 2016
SRR938938_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938938_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3854126
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT384730.9982289110423479No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT185320.4808353437329242No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT147650.38309593407169357No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC123530.3205136521224267No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT114880.29807017207013986No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC98940.25671189784661946No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC93960.24379068042923352No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT90500.23481328840831878No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86260.2238120912497412No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG85570.22202180208950095No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA80780.20959356284667394No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72570.18829171646178666No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC69290.1797813563957172No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA65210.16919529875255765No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC47310.12275156546516643No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT45900.1190931484855451No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45730.11865206275041346No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA45310.11756232152244114No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGCGT11500.029.2975353
CTTTGCG14200.024.061062
TTGCGTT16300.020.6700424
GTAAGCG6450.019.8646294-95
GAGTACT97900.019.65627912-13
GTACATG105050.018.772791
TACATGG101500.018.5139792
AGAGTAC156900.018.45003510-11
AGTACTT104600.018.44259312-13
GTACTTT103750.018.1362814-15
ACATGGG105700.016.9253233
GAGTACA92950.015.7843191
TACTTTT121550.015.51941314-15
AGTACAT84250.015.2077332
GTATAGG8500.015.1310611
ACTTTTT126750.015.05081316-17
CATGGGG68500.014.4093164
GTGTAGC22150.014.4087281
GTCTACG2651.3979418E-414.3803251
TATAGGG7500.013.9197682
Produced by FastQC (version 0.11.5)