##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938938_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3854126 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.14540546935933 33.0 31.0 34.0 30.0 34.0 2 32.37776061291198 34.0 31.0 34.0 30.0 34.0 3 32.48455058293372 34.0 31.0 34.0 30.0 34.0 4 36.04196775092459 37.0 35.0 37.0 35.0 37.0 5 35.95714592620999 37.0 35.0 37.0 35.0 37.0 6 35.998893134267014 37.0 35.0 37.0 35.0 37.0 7 35.94623346512283 37.0 35.0 37.0 35.0 37.0 8 35.9148061064947 37.0 35.0 37.0 35.0 37.0 9 37.593623820290254 39.0 37.0 39.0 35.0 39.0 10-11 37.50847286777858 39.0 37.0 39.0 35.0 39.0 12-13 37.44090618729123 39.0 37.0 39.0 34.0 39.0 14-15 38.682343934785735 40.0 38.0 41.0 34.0 41.0 16-17 38.68406754216131 40.0 38.0 41.0 34.0 41.0 18-19 38.78832308544142 40.0 38.0 41.0 34.0 41.0 20-21 38.77401659935352 40.0 38.0 41.0 34.0 41.0 22-23 38.71368878443518 40.0 38.0 41.0 34.0 41.0 24-25 38.64230749072553 40.0 38.0 41.0 34.0 41.0 26-27 38.48281491056598 40.0 38.0 41.0 34.0 41.0 28-29 38.31857053972807 40.0 38.0 41.0 34.0 41.0 30-31 38.18245874680797 40.0 38.0 41.0 33.0 41.0 32-33 38.04502213990928 40.0 37.5 41.0 33.0 41.0 34-35 38.01725047390771 40.0 38.0 41.0 33.0 41.0 36-37 37.92566472917595 40.0 37.5 41.0 33.0 41.0 38-39 37.80459006789088 40.0 37.0 41.0 33.0 41.0 40-41 37.69079280231108 40.0 37.0 41.0 33.0 41.0 42-43 37.5330610623524 40.0 37.0 41.0 32.0 41.0 44-45 37.3093353720143 40.0 37.0 41.0 31.5 41.0 46-47 37.05794841683951 40.0 36.0 41.0 31.0 41.0 48-49 36.79563784370309 39.5 36.0 41.0 30.5 41.0 50-51 36.53233858986447 39.0 36.0 41.0 30.0 41.0 52-53 36.32553904568766 39.0 35.0 40.5 30.0 41.0 54-55 36.08588328456309 39.0 35.0 40.0 29.0 41.0 56-57 35.76786877232348 38.5 35.0 40.0 28.5 41.0 58-59 35.45340928137793 38.0 34.5 40.0 28.0 41.0 60-61 34.98439659211972 38.0 34.0 40.0 27.5 41.0 62-63 34.67412222641398 37.0 34.0 40.0 27.0 41.0 64-65 34.41138808124073 37.0 34.0 39.5 26.0 41.0 66-67 33.919249656082854 36.0 33.0 39.0 26.0 40.5 68-69 33.46701392222258 36.0 33.0 39.0 26.0 40.0 70-71 32.95644914047958 35.0 32.0 38.0 25.0 40.0 72-73 32.41343381093405 35.0 31.5 37.0 23.5 39.5 74-75 31.945704810896167 35.0 31.0 37.0 22.5 39.0 76-77 29.980938739418484 33.0 28.5 35.0 20.0 37.0 78-79 30.974769117563877 34.0 30.5 36.0 20.0 37.5 80-81 30.953653694767635 34.0 31.0 35.5 20.0 37.0 82-83 30.620504752569065 34.0 30.5 35.0 19.5 37.0 84-85 30.198062154688248 34.0 30.0 35.0 18.0 36.0 86-87 29.637816329824194 34.0 29.5 35.0 10.5 36.0 88-89 29.041953869697046 34.0 29.0 35.0 6.0 35.0 90-91 28.54465435224484 33.5 29.0 35.0 2.0 35.0 92-93 28.166316046750936 33.0 28.0 35.0 2.0 35.0 94-95 27.723568845439928 33.0 27.0 35.0 2.0 35.0 96-97 27.035067094329555 33.0 26.0 34.5 2.0 35.0 98-99 26.06038463713952 32.5 24.5 34.0 2.0 35.0 100-101 21.504890213760525 27.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1581242610699718 1101 2 0.16497416464400771 1101 3 0.18980020666356268 1101 4 0.11410170303535239 1101 5 0.16829059646301658 1101 6 0.13163749067140174 1101 7 0.1059138571910907 1101 8 0.13775174429862602 1101 9 0.28254019968013466 1101 10-11 0.23889054523302633 1101 12-13 0.25066459975911215 1101 14-15 0.28077339873021856 1101 16-17 0.28828986673882184 1101 18-19 0.35596735167982985 1101 20-21 0.32704464183530746 1101 22-23 0.2817509268503642 1101 24-25 0.2657128859591822 1101 26-27 0.24351872568734478 1101 28-29 0.34782064283849223 1101 30-31 0.1913492610809655 1101 32-33 0.2651198459760451 1101 34-35 0.3915832432690607 1101 36-37 0.30233422026795864 1101 38-39 0.32212218323855524 1101 40-41 0.45355423150951424 1101 42-43 0.5258514848174656 1101 44-45 0.42244860001299145 1101 46-47 0.40053889645017904 1101 48-49 0.3057771214836933 1101 50-51 0.22349671354175626 1101 52-53 0.23768615302664386 1101 54-55 0.3594523220056729 1101 56-57 0.21211203714074855 1101 58-59 0.3859441547086959 1101 60-61 0.4721885190352282 1101 62-63 0.38056879139724487 1101 64-65 0.3959431747367006 1101 66-67 0.4338666364594488 1101 68-69 0.3955550144167077 1101 70-71 0.4242621267529856 1101 72-73 0.3506606625826194 1101 74-75 0.457524288456046 1101 76-77 0.32970140556928484 1101 78-79 0.485559494170154 1101 80-81 0.6797136609587362 1101 82-83 0.5424423441922599 1101 84-85 0.4435926863741706 1101 86-87 0.48536646756532065 1101 88-89 0.7124238234757456 1101 90-91 0.5952912913897102 1101 92-93 0.6035151522599342 1101 94-95 0.7603286345465712 1101 96-97 0.7990188506529741 1101 98-99 0.7793795384619742 1101 100-101 1.3156245504559685 1102 1 0.18893589900152818 1102 2 0.1368371896017493 1102 3 0.1972901600405308 1102 4 0.11857168759005532 1102 5 0.15442033458682403 1102 6 0.18149793263906844 1102 7 0.1475249419153144 1102 8 0.258960706879364 1102 9 0.3341801112435334 1102 10-11 0.2760955770986797 1102 12-13 0.2700622988760628 1102 14-15 0.3274033833088481 1102 16-17 0.3089416739345836 1102 18-19 0.18456273567678494 1102 20-21 0.2711783591277026 1102 22-23 0.33195405766404207 1102 24-25 0.3205162574850462 1102 26-27 0.31409042011208044 1102 28-29 0.29007603942137905 1102 30-31 0.22767412920969576 1102 32-33 0.36869652153981036 1102 34-35 0.37910796352489484 1102 36-37 0.36156069310469974 1102 38-39 0.36187296776645894 1102 40-41 0.36900153504254973 1102 42-43 0.30982703135495626 1102 44-45 0.3010152409175575 1102 46-47 0.40888067425081687 1102 48-49 0.25811936946270464 1102 50-51 0.3609613753901968 1102 52-53 0.31938996338563896 1102 54-55 0.34768006659712825 1102 56-57 0.2736657732642058 1102 58-59 0.23694651397521227 1102 60-61 0.19524478610611595 1102 62-63 0.19804566831221138 1102 64-65 0.2928802707332423 1102 66-67 0.3164647876893838 1102 68-69 0.4652555179741569 1102 70-71 0.4333893297420204 1102 72-73 0.4702197387598943 1102 74-75 0.5261497674577349 1102 76-77 0.3486305713436515 1102 78-79 0.36999491523103245 1102 80-81 0.5021588905625087 1102 82-83 0.6356947611842436 1102 84-85 0.544079255184716 1102 86-87 0.8000130770768017 1102 88-89 0.9404258615135355 1102 90-91 0.9556156396901905 1102 92-93 0.8251065235895787 1102 94-95 0.8999802090459141 1102 96-97 0.8462707298709695 1102 98-99 0.9852809499541877 1102 100-101 1.5317761943455004 1103 1 0.029942926974989348 1103 2 0.06691816666176464 1103 3 0.006446725988162427 1103 4 0.002975367398697415 1103 5 0.07580375631692249 1103 6 -0.02003837083344706 1103 7 0.05931419064283716 1103 8 0.061251719878356425 1103 9 0.07796676540176861 1103 10-11 0.07719628613439511 1103 12-13 0.10534153441982141 1103 14-15 0.17142224607115963 1103 16-17 0.0773103422081789 1103 18-19 0.07486253260287157 1103 20-21 0.07258879598614953 1103 22-23 0.09045766641462905 1103 24-25 0.13178291617055038 1103 26-27 0.046973399808848626 1103 28-29 0.19826277592614616 1103 30-31 0.20789922755319168 1103 32-33 0.15747551438112595 1103 34-35 0.11921568538374316 1103 36-37 0.056705708555064405 1103 38-39 0.19434296713073707 1103 40-41 0.1885008045587071 1103 42-43 0.24980411127327784 1103 44-45 0.18711178509622783 1103 46-47 0.24673242197574297 1103 48-49 0.09635797061734053 1103 50-51 0.09462485731761916 1103 52-53 0.23629757485498004 1103 54-55 0.23848899650216282 1103 56-57 0.2155044507052324 1103 58-59 0.13124477418193692 1103 60-61 0.07837331264385483 1103 62-63 0.17056518699494916 1103 64-65 0.13779077532260686 1103 66-67 0.2032840920097314 1103 68-69 0.16508718585978244 1103 70-71 0.183734519748036 1103 72-73 0.29673728997843085 1103 74-75 0.25071343035741833 1103 76-77 0.20444675558599457 1103 78-79 0.2512535635477704 1103 80-81 0.29633136584815034 1103 82-83 0.4269426275119379 1103 84-85 0.3717121013505782 1103 86-87 0.29615928465065977 1103 88-89 0.6142177039191488 1103 90-91 0.571038585081844 1103 92-93 0.4672198305751927 1103 94-95 0.3340926979133876 1103 96-97 0.23089208435852981 1103 98-99 0.3535868408800873 1103 100-101 0.9458498760731864 1104 1 0.009070223502590125 1104 2 0.03951383542106868 1104 3 0.06414783825558601 1104 4 0.013617764289080014 1104 5 0.026861644183618694 1104 6 0.05446484970334353 1104 7 0.0016614318491434688 1104 8 0.07389984491811674 1104 9 0.10645608199852319 1104 10-11 0.036076832279640314 1104 12-13 -0.015114243396837423 1104 14-15 0.09414745943267633 1104 16-17 0.07724219131667809 1104 18-19 0.08616472277635268 1104 20-21 0.07917514714688423 1104 22-23 0.15418946301473824 1104 24-25 0.12733848636405298 1104 26-27 0.1040304988190286 1104 28-29 0.0663126607862452 1104 30-31 0.03835729057818327 1104 32-33 0.1635604877935961 1104 34-35 0.20899125649943784 1104 36-37 0.14191372223128695 1104 38-39 0.23420099612009437 1104 40-41 0.2420230691979981 1104 42-43 0.1546227955187902 1104 44-45 0.23700652580905768 1104 46-47 0.22381653088890374 1104 48-49 0.296115291192109 1104 50-51 0.18722841898527776 1104 52-53 0.03448045420734758 1104 54-55 0.13040153780801944 1104 56-57 0.18215166499140167 1104 58-59 0.19291427341150325 1104 60-61 0.2497924574200141 1104 62-63 0.2513795130579126 1104 64-65 0.3712446762571915 1104 66-67 0.4172800990841026 1104 68-69 0.29735069376098267 1104 70-71 0.10557083990970284 1104 72-73 0.24235056222520512 1104 74-75 0.22195885731677478 1104 76-77 0.13234499439757386 1104 78-79 0.31139012012752687 1104 80-81 0.329212777578487 1104 82-83 0.4535236829539002 1104 84-85 0.3303912350097882 1104 86-87 0.21139094013534532 1104 88-89 0.4049702178835659 1104 90-91 0.5334196548995571 1104 92-93 0.490966634124657 1104 94-95 0.5167732436677532 1104 96-97 0.45057617547934825 1104 98-99 0.6016766434147769 1104 100-101 1.0765165611740137 1105 1 -0.0023063461952972375 1105 2 0.029612608050314293 1105 3 0.06471651385158594 1105 4 0.08887551186295894 1105 5 0.09941631600973011 1105 6 0.06170289466249557 1105 7 0.028581042652319866 1105 8 0.06776814670431008 1105 9 0.027086476354462263 1105 10-11 0.06011416732229691 1105 12-13 0.05180665025086739 1105 14-15 0.13005195976366224 1105 16-17 0.11887486983277995 1105 18-19 0.05610473841652919 1105 20-21 0.15526400589098444 1105 22-23 0.02876602298461961 1105 24-25 0.03466960866315816 1105 26-27 0.04367604119382662 1105 28-29 0.08853797792226459 1105 30-31 0.08516916946258135 1105 32-33 -0.03476621075408559 1105 34-35 0.06362587727345215 1105 36-37 0.11222877789813168 1105 38-39 0.2123693145676171 1105 40-41 0.1679906017112387 1105 42-43 0.18942464688247895 1105 44-45 0.24492030414959487 1105 46-47 0.302142509792084 1105 48-49 0.20362234239261312 1105 50-51 0.2768518564696123 1105 52-53 0.39078952843377124 1105 54-55 0.35352838034147993 1105 56-57 0.25870022154596484 1105 58-59 0.2690355232690109 1105 60-61 0.25580710673661855 1105 62-63 0.2597649714319914 1105 64-65 0.20817522466114724 1105 66-67 0.3442594716693037 1105 68-69 0.19068988617620874 1105 70-71 0.256206121732653 1105 72-73 0.2059118751895639 1105 74-75 0.25781471764430464 1105 76-77 0.14083000833428727 1105 78-79 0.37771382262165787 1105 80-81 0.30658241152477217 1105 82-83 0.3599662007453688 1105 84-85 0.10637216130176341 1105 86-87 0.12544803624607326 1105 88-89 0.33547449738609103 1105 90-91 0.4369198080373451 1105 92-93 0.48614213600380296 1105 94-95 0.4070317247528017 1105 96-97 0.4209832133749565 1105 98-99 0.5623115206154807 1105 100-101 1.0676534494326368 1106 1 0.03243580997787632 1106 2 -0.08550161235456955 1106 3 -0.10692335519875229 1106 4 -0.04165768023766958 1106 5 -0.003109760914959736 1106 6 -0.024117110559146226 1106 7 5.647369347485665E-4 1106 8 9.777941996702566E-4 1106 9 -0.06552293545299648 1106 10-11 0.016788292702635488 1106 12-13 0.021394971249904415 1106 14-15 0.03950508223957172 1106 16-17 0.006637889878696512 1106 18-19 -0.11721622209120852 1106 20-21 -8.217905016181248E-4 1106 22-23 0.1116072322704369 1106 24-25 0.021738657980094445 1106 26-27 0.05538419031276476 1106 28-29 0.07994906328493556 1106 30-31 -0.008018292065280264 1106 32-33 -0.025203169400249692 1106 34-35 -0.018487441115119907 1106 36-37 -0.09260061136139086 1106 38-39 0.08851234399777752 1106 40-41 0.14648236596083564 1106 42-43 0.1753772221323402 1106 44-45 -0.016732224518356986 1106 46-47 0.08146693004388084 1106 48-49 0.04427768598207393 1106 50-51 0.10096259910284289 1106 52-53 0.22681283904078242 1106 54-55 0.10720305934590613 1106 56-57 0.11382780499837963 1106 58-59 0.16146337946135958 1106 60-61 0.07286743140168994 1106 62-63 0.15694327720088808 1106 64-65 0.21151001269225844 1106 66-67 0.18929113909442208 1106 68-69 0.10031518991477384 1106 70-71 0.023130239035914713 1106 72-73 0.2324632085710192 1106 74-75 0.36848421537484555 1106 76-77 0.07339628256718456 1106 78-79 0.23396548001212025 1106 80-81 0.3555190764624392 1106 82-83 0.4022957499556661 1106 84-85 0.2761433104524471 1106 86-87 0.36105948333431925 1106 88-89 0.5520505299946983 1106 90-91 0.7770075649845616 1106 92-93 0.7465874870284104 1106 94-95 0.8007993498256489 1106 96-97 0.8606965476560973 1106 98-99 0.8021479657366015 1106 100-101 1.260589146676967 1107 1 -0.06651922545895417 1107 2 -0.07551101482059863 1107 3 -0.09706642391618914 1107 4 -0.10549744571832065 1107 5 -0.11388929485511312 1107 6 -0.004342907229101911 1107 7 -0.07547073732456511 1107 8 -0.07291825622993287 1107 9 -0.0634759322885401 1107 10-11 -0.023872599072767287 1107 12-13 0.02175968916446891 1107 14-15 -0.027020229183690958 1107 16-17 -0.027334724579716863 1107 18-19 -0.07838044750292283 1107 20-21 -0.08534812695408789 1107 22-23 -0.013347416679984292 1107 24-25 -0.11310805738182239 1107 26-27 -0.11554706627984501 1107 28-29 -0.1185575409735975 1107 30-31 -0.11645675708787451 1107 32-33 -0.08836925697379883 1107 34-35 -0.19507774488885588 1107 36-37 -0.027451680424071867 1107 38-39 -0.058319269369150106 1107 40-41 -0.18499427948980696 1107 42-43 -0.24440278613205635 1107 44-45 -0.2771587649837173 1107 46-47 -0.5117755917112561 1107 48-49 -0.5241754746901108 1107 50-51 -0.3386931988426696 1107 52-53 -0.23483831342964123 1107 54-55 -0.424506394524343 1107 56-57 -0.3353641252263131 1107 58-59 -0.41227112575582225 1107 60-61 -0.5232267643513921 1107 62-63 -0.36387428669731037 1107 64-65 -0.299186724552726 1107 66-67 -0.20234448517661008 1107 68-69 -0.2843220513360052 1107 70-71 -0.222473477955198 1107 72-73 -0.1665870736175279 1107 74-75 -0.3190365506320738 1107 76-77 -0.5301492338466502 1107 78-79 -0.2443786777415511 1107 80-81 -0.09500493343344729 1107 82-83 -0.1259494565816084 1107 84-85 -0.08656770082058429 1107 86-87 -0.13073684596127322 1107 88-89 0.07782902133809344 1107 90-91 0.22825108121660875 1107 92-93 0.19344827782850516 1107 94-95 0.20065888569058998 1107 96-97 0.10702168050125493 1107 98-99 -0.21515990430314247 1107 100-101 0.42745122067307406 1108 1 -0.05821438697309134 1108 2 -0.07566242681694746 1108 3 -0.029612344214271502 1108 4 -0.05406350416518535 1108 5 -0.02730219332019601 1108 6 -0.003541688358993156 1108 7 -0.04517922365961624 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-1.0360575781756225 2316 48-49 -1.1540316266565114 2316 50-51 -1.0977283161292632 2316 52-53 -1.0563391135362608 2316 54-55 -1.18282036608381 2316 56-57 -1.232115854378577 2316 58-59 -1.1845218945975802 2316 60-61 -1.085752181315108 2316 62-63 -1.1231944391146556 2316 64-65 -1.0391275403967626 2316 66-67 -1.1550311637893458 2316 68-69 -1.2864559067088592 2316 70-71 -1.2946726027216258 2316 72-73 -1.1813358159485112 2316 74-75 -1.5093417447526356 2316 76-77 -1.46760892613651 2316 78-79 -1.6018936894396347 2316 80-81 -1.6238594345238404 2316 82-83 -1.7849097150621454 2316 84-85 -1.8753193850153487 2316 86-87 -1.8903996567043357 2316 88-89 -1.9223182601250848 2316 90-91 -2.0977611301082995 2316 92-93 -2.2029898613325365 2316 94-95 -2.5151275990343542 2316 96-97 -2.627939007928191 2316 98-99 -2.8103823766189038 2316 100-101 -3.6351466817057023 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 13.0 4 32.0 5 80.0 6 168.0 7 432.0 8 986.0 9 1818.0 10 3241.0 11 4673.0 12 6713.0 13 9128.0 14 11040.0 15 15011.0 16 19111.0 17 25778.0 18 25880.0 19 20571.0 20 20421.0 21 21683.0 22 23623.0 23 26816.0 24 31344.0 25 37264.0 26 45146.0 27 54552.0 28 66993.0 29 81213.0 30 98546.0 31 120887.0 32 148634.0 33 190461.0 34 255397.0 35 366460.0 36 582235.0 37 884071.0 38 614869.0 39 38830.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.53558499382745 19.485006513810294 15.101098868464796 22.878309623897465 2 15.511921509571819 21.46362106480172 38.644429372573704 24.380028053052754 3 20.80357518150678 23.73427853682002 29.783899125249146 25.67824715642405 4 13.393672132151362 17.077438568432896 37.388632338434185 32.14025696098155 5 13.885481272988434 37.32257910512201 34.29874378326318 14.493195838626383 6 31.668943879883532 36.41852912956141 16.922383959424263 14.990143031130792 7 28.047059177618998 32.431892470562715 20.952247020465858 18.568801331352425 8 25.57972936017141 34.24431375621866 19.683969854644086 20.49198702896584 9 24.891531828487185 18.11585817381165 21.1850105575168 35.80759944018436 10-11 25.151201595381156 25.99361048393332 27.2567502982518 21.598437622433725 12-13 26.674659832086444 23.459041038097872 27.29498205299982 22.57131707681586 14-15 22.796889260502056 25.281031014635836 26.5897133353269 25.33236638953521 16-17 22.115925257554743 28.224017166424282 26.94945686793192 22.710600708089054 18-19 21.961178747140078 28.134926050679194 28.06627235331694 21.837622848863788 20-21 23.22168502015762 26.242797978062992 27.754320953700006 22.781196048079384 22-23 22.505193135875682 26.298634242886713 28.106021961918216 23.090150659319388 24-25 23.35427020289425 26.312022492258947 27.838685087098863 22.49502221774794 26-27 22.871449973353233 25.876852495222003 28.952231971658428 22.299465559766336 28-29 22.13664005795348 27.682566683081976 27.97893737776087 22.201855881203677 30-31 22.71557805842362 26.36279924423851 27.702220944515048 23.21940175282282 32-33 22.709337992582494 26.50572399553102 28.06503990788054 22.71989810400594 34-35 23.6008493417368 26.277311088990047 27.857897815631816 22.26394175364134 36-37 23.10324037836914 25.939698569393688 28.629264901084124 22.327796151153045 38-39 22.507444212198 26.838827453865342 28.30321265600409 22.350515677932563 40-41 22.78827435938851 26.869778602022688 27.852749036236723 22.489198002352083 42-43 22.403217041174717 26.890801109104444 27.21435260964264 23.491629240078197 44-45 22.9503958112249 26.088707898840457 27.55073155670181 23.410164733232836 46-47 23.66357231408068 26.826786310123353 27.371398592362766 22.138242783433203 48-49 23.182346778173372 26.6311643805405 27.525856758926427 22.6606320823597 50-51 22.0265711080973 27.025752501679374 28.345641835432346 22.602034554790976 52-53 22.95532112857753 27.54568740098274 26.68293667617509 22.81605479426464 54-55 22.545215179783952 26.637712415214242 27.23185490043657 23.585217504565236 56-57 23.128628903154695 26.515804101889767 27.180792740040154 23.17477425491538 58-59 23.95761413450299 26.412185417404515 26.938467067017978 22.691733381074513 60-61 22.950887516620543 26.846500445441606 28.021111869478275 22.18150016845957 62-63 22.417832214099903 26.939946955548415 27.83213366662117 22.810087163730504 64-65 22.256257984969835 27.73734625527618 27.193317481881706 22.813078277872282 66-67 22.84810048578421 26.714161819527497 26.663540107846817 23.77419758684147 68-69 22.39699133565071 26.750520034799585 27.329492544113766 23.52299608543594 70-71 23.37103146082925 26.64451032477921 26.855141736414428 23.129316477977106 72-73 22.9615482213088 26.052274886705835 27.900476009346864 23.085700882638502 74-75 22.251724515493265 27.826762799140454 26.862406677934246 23.059106007432035 76-77 22.59668371220461 26.544330543414034 26.889618614044362 23.96936713033699 78-79 22.754625953913262 26.69391280612663 27.23210254781631 23.31935869214379 80-81 23.302753983652842 26.46433977508779 26.963895316344093 23.269010924915275 82-83 22.81034662592764 26.307624608017488 27.761741572538106 23.120287193516766 84-85 22.502870949211314 26.897083800581505 27.342372823306764 23.25767242690042 86-87 22.40404308648218 27.037978446365102 27.060494027979086 23.49748443917363 88-89 22.327167443266514 26.9846686681052 26.505860983415165 24.18230290521312 90-91 22.659795024889352 26.160718783588194 26.877466496906287 24.302019694616163 92-93 23.108494636656925 26.731287456611437 27.00975525968793 23.150462647043714 94-95 22.765096418747078 26.47509448315909 27.119416957307575 23.640392140786265 96-97 21.73076611221419 26.534115270675855 27.94074625674849 23.79437236036146 98-99 22.343158993764085 26.985988522430247 26.62873502319333 24.042117460612342 100-101 22.270485161459728 25.776111220229573 27.494830854989168 24.458572763321527 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 562.0 1 383.0 2 261.0 3 303.0 4 280.5 5 343.0 6 538.0 7 1062.0 8 1800.5 9 2227.0 10 2136.5 11 2026.5 12 2086.5 13 2281.5 14 2597.0 15 2858.0 16 3107.5 17 3401.0 18 3771.5 19 4225.0 20 4797.0 21 5734.0 22 7203.5 23 9102.0 24 11268.0 25 14005.5 26 18388.0 27 23713.0 28 28441.5 29 33149.0 30 38189.0 31 44633.5 32 51430.5 33 56949.5 34 62597.5 35 69455.5 36 77362.0 37 84542.0 38 91062.0 39 96707.5 40 102051.5 41 107256.5 42 111247.5 43 117230.0 44 124752.0 45 131383.0 46 139454.5 47 172178.5 48 227744.5 49 223078.0 50 172144.5 51 149088.5 52 143153.5 53 138879.5 54 133236.5 55 123373.5 56 111943.0 57 101726.0 58 92064.5 59 82014.5 60 68874.0 61 56054.5 62 44050.5 63 32696.0 64 24110.0 65 17529.5 66 12263.0 67 9024.5 68 6719.0 69 4910.5 70 3499.0 71 2185.0 72 1334.5 73 889.0 74 554.5 75 325.0 76 186.5 77 91.0 78 52.0 79 23.5 80 11.5 81 13.0 82 10.0 83 6.5 84 4.0 85 3.0 86 1.0 87 0.5 88 1.0 89 1.0 90 0.5 91 0.5 92 0.5 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.39850798858158765 2 0.0 3 0.0 4 0.0 5 3.6324707599076937E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0015178538532471435 16-17 0.002361105993940001 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0021405631263741766 36-37 0.007018452432535936 38-39 0.011961207287981763 40-41 0.0024259715432240667 42-43 6.616286026974728E-4 44-45 4.1513951541802214E-4 46-47 2.0756975770901107E-4 48-49 2.0756975770901107E-4 50-51 3.891932957043958E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 2.8540841684989023E-4 60-61 0.002218401785515056 62-63 0.0 64-65 2.594621971362638E-4 66-67 0.0012583916561108796 68-69 0.0014789345236767039 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.12276453857502324 78-79 5.189243942725277E-5 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.14737452797339787 88-89 0.4381278660842951 90-91 0.3379624848798405 92-93 0.0 94-95 0.0 96-97 6.097361632702201E-4 98-99 0.0 100-101 1.8162353799538468E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3854126.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.22450126455673 #Duplication Level Percentage of deduplicated Percentage of total 1 70.30456912435928 33.904027826417796 2 15.24633083863198 14.704934016149165 3 5.805530788127243 8.399064805003956 4 2.802023987787942 5.405048373695915 5 1.6411848653760799 3.957266080785007 6 0.994509089286672 2.8775822900350962 7 0.6684764783754029 2.256586134371873 8 0.4665614798086913 1.7999755738422152 9 0.358934926580928 1.5578512038715984 >10 1.5896203779403577 13.24947904730768 >50 0.08517402533585411 2.7657950783874665 >100 0.033908883004320683 2.80523147878788 >500 0.0012377646654864285 0.37711472472498075 >1k 0.0011839487732277789 1.53023294547399 >5k 4.843426799568185E-4 1.9284729357780515 >10k+ 2.6907926664267694E-4 2.481337485367326 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 38473 0.9982289110423479 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 18532 0.4808353437329242 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 14765 0.38309593407169357 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 12353 0.3205136521224267 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 11488 0.29807017207013986 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 9894 0.25671189784661946 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 9396 0.24379068042923352 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 9050 0.23481328840831878 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8626 0.2238120912497412 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 8557 0.22202180208950095 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 8078 0.20959356284667394 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7257 0.18829171646178666 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 6929 0.1797813563957172 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 6521 0.16919529875255765 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 4731 0.12275156546516643 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 4590 0.1190931484855451 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4573 0.11865206275041346 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 4531 0.11756232152244114 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.5946219713626384E-5 2 0.0 0.0 0.0 0.0 2.5946219713626384E-5 3 0.0 0.0 0.0 0.0 2.5946219713626384E-5 4 0.0 0.0 0.0 0.0 2.5946219713626384E-5 5 0.0 0.0 0.0 0.0 2.5946219713626384E-5 6 0.0 0.0 0.0 0.0 2.5946219713626384E-5 7 0.0 0.0 0.0 0.0 2.5946219713626384E-5 8 0.0 0.0 0.0 2.5946219713626384E-5 2.5946219713626384E-5 9 0.0 0.0 0.0 2.5946219713626384E-5 5.189243942725277E-5 10-11 0.0 0.0 0.0 2.5946219713626384E-5 5.189243942725277E-5 12-13 2.5946219713626384E-5 0.0 0.0 3.8919329570439574E-5 7.783865914087915E-5 14-15 2.5946219713626384E-5 0.0 0.0 6.486554928406595E-5 9.081176899769234E-5 16-17 2.5946219713626384E-5 0.0 0.0 1.297310985681319E-4 1.4270420842494512E-4 18-19 2.5946219713626384E-5 0.0 0.0 2.2054286756582425E-4 1.8162353799538468E-4 20-21 2.5946219713626384E-5 0.0 0.0 3.891932957043958E-4 2.0756975770901107E-4 22-23 2.5946219713626384E-5 0.0 0.0 7.394672618383519E-4 2.0756975770901107E-4 24-25 2.5946219713626384E-5 0.0 0.0 0.0010378487885450553 2.8540841684989023E-4 26-27 2.5946219713626384E-5 0.0 0.0 0.0017124505010993414 2.9838152670670344E-4 28-29 2.5946219713626384E-5 0.0 0.0 0.0036973363091917597 3.113546365635166E-4 30-31 2.5946219713626384E-5 0.0 0.0 0.009664966843325828 3.113546365635166E-4 32-33 2.5946219713626384E-5 0.0 0.0 0.02178185144958935 3.37300856277143E-4 34-35 2.5946219713626384E-5 0.0 0.0 0.039567985063280235 3.6324707599076937E-4 36-37 2.5946219713626384E-5 0.0 0.0 0.06734341326671728 3.6324707599076937E-4 38-39 5.189243942725277E-5 0.0 0.0 0.10789735467911532 3.891932957043958E-4 40-41 7.783865914087915E-5 0.0 0.0 0.15266755679497762 3.891932957043958E-4 42-43 7.783865914087915E-5 0.0 0.0 0.20392431383924658 4.2811262527483535E-4 44-45 7.783865914087915E-5 0.0 0.0 0.26841364293746495 4.929781745589012E-4 46-47 7.783865914087915E-5 0.0 0.0 0.34601878610092146 5.318975041293408E-4 48-49 7.783865914087915E-5 0.0 0.0 0.42708774959614704 5.44870613986154E-4 50-51 7.783865914087915E-5 0.0 0.0 0.5149546226563428 5.578437238429673E-4 52-53 7.783865914087915E-5 0.0 0.0 0.6049361126231991 6.746017125542859E-4 54-55 7.783865914087915E-5 0.0 0.0 0.7096420822775384 7.264941519815387E-4 56-57 7.783865914087915E-5 0.0 0.0 0.8485192232947236 7.264941519815387E-4 58-59 7.783865914087915E-5 0.0 0.0 0.9848795809996871 7.264941519815387E-4 60-61 7.783865914087915E-5 0.0 0.0 1.136197934369556 7.264941519815387E-4 62-63 7.783865914087915E-5 0.0 0.0 1.2917455215527465 7.264941519815387E-4 64-65 7.783865914087915E-5 0.0 0.0 1.461900830434708 7.264941519815387E-4 66-67 7.783865914087915E-5 0.0 0.0 1.6612067171649292 7.394672618383519E-4 68-69 7.783865914087915E-5 0.0 0.0 1.8901302136982547 7.524403716951652E-4 70-71 7.783865914087915E-5 0.0 0.0 2.1382668859295206 7.783865914087916E-4 72-73 7.783865914087915E-5 0.0 0.0 2.3924749735737754 8.043328111224179E-4 74-75 7.783865914087915E-5 0.0 0.0 2.6744065969820396 8.432521406928575E-4 76-77 7.783865914087915E-5 0.0 0.0 2.967417256208022 8.562252505496707E-4 78-79 7.783865914087915E-5 1.2973109856813192E-5 0.0 3.3289259354779785 8.562252505496707E-4 80-81 7.783865914087915E-5 2.5946219713626384E-5 0.0 3.7145775721914642 8.951445801201102E-4 82-83 9.081176899769234E-5 2.5946219713626384E-5 0.0 4.094546986787666 9.600101294041763E-4 84-85 1.0378487885450554E-4 2.5946219713626384E-5 0.0 4.48647760867185 9.600101294041763E-4 86-87 1.297310985681319E-4 2.5946219713626384E-5 0.0 4.908972877378684 9.729832392609895E-4 88-89 1.297310985681319E-4 2.5946219713626384E-5 0.0 5.369077191560422 9.989294589746157E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTTGCGT 1150 0.0 29.297535 3 CTTTGCG 1420 0.0 24.06106 2 TTGCGTT 1630 0.0 20.670042 4 GTAAGCG 645 0.0 19.86462 94-95 GAGTACT 9790 0.0 19.656279 12-13 GTACATG 10505 0.0 18.77279 1 TACATGG 10150 0.0 18.513979 2 AGAGTAC 15690 0.0 18.450035 10-11 AGTACTT 10460 0.0 18.442593 12-13 GTACTTT 10375 0.0 18.13628 14-15 ACATGGG 10570 0.0 16.925323 3 GAGTACA 9295 0.0 15.784319 1 TACTTTT 12155 0.0 15.519413 14-15 AGTACAT 8425 0.0 15.207733 2 GTATAGG 850 0.0 15.131061 1 ACTTTTT 12675 0.0 15.050813 16-17 CATGGGG 6850 0.0 14.409316 4 GTGTAGC 2215 0.0 14.408728 1 GTCTACG 265 1.3979418E-4 14.380325 1 TATAGGG 750 0.0 13.919768 2 >>END_MODULE