##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938937_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2348551 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.78985425481499 33.0 31.0 34.0 30.0 34.0 2 31.843823276564997 33.0 31.0 34.0 30.0 34.0 3 31.874239052079346 34.0 31.0 34.0 30.0 34.0 4 35.41692090144093 37.0 35.0 37.0 33.0 37.0 5 35.471958667280376 37.0 35.0 37.0 33.0 37.0 6 35.572252848671376 37.0 35.0 37.0 33.0 37.0 7 35.50707649099381 37.0 35.0 37.0 33.0 37.0 8 35.50170935185142 37.0 35.0 37.0 33.0 37.0 9 37.0858797616062 39.0 37.0 39.0 33.0 39.0 10-11 36.97226396190673 39.0 37.0 39.0 33.0 39.0 12-13 36.8532269471687 39.0 37.0 39.0 33.0 39.0 14-15 37.97789253884629 40.0 38.0 41.0 33.0 41.0 16-17 37.90742930428166 40.0 38.0 41.0 33.0 41.0 18-19 37.890765838170005 40.0 38.0 41.0 33.0 41.0 20-21 38.02180259232182 40.0 38.0 41.0 33.0 41.0 22-23 37.962753416894074 40.0 38.0 41.0 33.0 41.0 24-25 37.87344345513468 40.0 38.0 41.0 32.5 41.0 26-27 37.71002716142847 40.0 37.5 41.0 32.0 41.0 28-29 37.57053732280031 40.0 37.0 41.0 32.0 41.0 30-31 37.368103141043136 40.0 37.0 41.0 31.5 41.0 32-33 37.141182158701255 40.0 36.0 41.0 31.0 41.0 34-35 36.804198205617 40.0 36.0 41.0 30.5 41.0 36-37 36.505474013551336 39.0 35.0 41.0 30.0 41.0 38-39 36.23222127175437 39.0 35.0 41.0 30.0 41.0 40-41 36.05749438696456 39.0 35.0 41.0 29.5 41.0 42-43 35.84868031394677 39.0 35.0 41.0 28.5 41.0 44-45 35.71647581849404 39.0 35.0 41.0 27.5 41.0 46-47 35.40727027005162 39.0 35.0 41.0 25.5 41.0 48-49 35.10856332266151 39.0 35.0 41.0 24.0 41.0 50-51 33.98445999256563 38.0 34.0 40.0 23.0 40.5 52-53 34.05499007686016 38.0 33.5 39.5 23.0 40.5 54-55 34.554288367593465 38.5 34.0 40.0 23.0 41.0 56-57 34.49259713755417 38.5 34.0 40.0 23.0 41.0 58-59 34.29427378839122 38.0 34.0 40.0 22.0 41.0 60-61 33.96070130050401 38.0 34.0 40.0 21.5 41.0 62-63 33.56476163387553 37.5 33.5 40.0 19.5 41.0 64-65 33.13688972477072 37.0 33.0 40.0 18.0 41.0 66-67 32.723369004973705 36.0 32.0 39.0 14.0 41.0 68-69 32.29709616695571 36.0 32.0 39.0 12.0 40.5 70-71 31.814267180061236 35.0 31.5 38.5 8.0 40.0 72-73 31.341158016155493 35.0 31.0 37.5 7.0 40.0 74-75 30.871641492988655 35.0 31.0 37.0 2.0 39.0 76-77 30.407194052843646 35.0 30.0 36.5 2.0 39.0 78-79 30.00033552603286 34.5 30.0 36.0 2.0 38.5 80-81 29.56394623748856 34.0 29.5 35.5 2.0 37.0 82-83 29.08712861675135 34.0 29.0 35.0 2.0 37.0 84-85 28.66410501624193 34.0 29.0 35.0 2.0 36.5 86-87 28.189974797226036 34.0 28.5 35.0 2.0 36.0 88-89 27.664234244859916 34.0 26.5 35.0 2.0 36.0 90-91 27.161802106916134 33.5 25.5 35.0 2.0 35.0 92-93 26.676393444298206 33.0 25.0 35.0 2.0 35.0 94-95 26.14682052891336 33.0 24.5 35.0 2.0 35.0 96-97 25.447298355454066 32.5 20.5 35.0 2.0 35.0 98-99 24.568581010163285 32.0 9.0 35.0 2.0 35.0 100-101 21.38055230650729 28.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.245385498609604 1101 2 0.46238740150026203 1101 3 0.3726215054097253 1101 4 0.39617987343704186 1101 5 0.21815244179444448 1101 6 0.3231148467365088 1101 7 0.4066536140937629 1101 8 0.2995439077164761 1101 9 0.44728691239753715 1101 10-11 0.3649095017769639 1101 12-13 0.3857195594028653 1101 14-15 0.5429099091862213 1101 16-17 0.5477950443943129 1101 18-19 0.5969645401688126 1101 20-21 0.5586557662080764 1101 22-23 0.7136716651237478 1101 24-25 0.6101343304414897 1101 26-27 0.5844309207594094 1101 28-29 0.5871758684934392 1101 30-31 0.6052571021431277 1101 32-33 0.5645564011882271 1101 34-35 0.5636368474450322 1101 36-37 0.5137117073612529 1101 38-39 0.7786153044123054 1101 40-41 0.6407595663231476 1101 42-43 0.7435939378592238 1101 44-45 0.8547878869308576 1101 46-47 0.8386571457676837 1101 48-49 0.8092734888730746 1101 50-51 0.6441255275742037 1101 52-53 0.6887386662117336 1101 54-55 0.8767492669947003 1101 56-57 0.7231130296526516 1101 58-59 0.6047054273438519 1101 60-61 0.7226924656902298 1101 62-63 0.6885511255940386 1101 64-65 0.653658986023494 1101 66-67 0.6979339102143101 1101 68-69 0.6364434317141132 1101 70-71 0.5991977202363401 1101 72-73 0.513659162148425 1101 74-75 0.3808748596527636 1101 76-77 0.3618040822826991 1101 78-79 0.3576082808220207 1101 80-81 0.34257309438995875 1101 82-83 0.42466726781936615 1101 84-85 0.5593743483348383 1101 86-87 0.5372426306309777 1101 88-89 0.2742880810878674 1101 90-91 0.38759690596495133 1101 92-93 0.29927247886758224 1101 94-95 0.14435422941608422 1101 96-97 -0.12918702610611987 1101 98-99 0.16069175821350612 1101 100-101 0.39210186068905273 1102 1 0.3224233650457826 1102 2 0.3233822875997454 1102 3 0.45028993345825796 1102 4 0.21419900636571043 1102 5 0.44316602866200583 1102 6 0.34854291491311784 1102 7 0.5137620012834816 1102 8 0.43747844193246266 1102 9 0.5667577120910963 1102 10-11 0.46121682035136047 1102 12-13 0.5071504693628555 1102 14-15 0.7150015010432611 1102 16-17 0.7806412148620367 1102 18-19 0.7225903545623851 1102 20-21 0.674124169374636 1102 22-23 0.7321206668982327 1102 24-25 0.8143099186055238 1102 26-27 0.8643030405071741 1102 28-29 0.7437656629552336 1102 30-31 0.8021982562738614 1102 32-33 0.6573239315213186 1102 34-35 0.7328987695120688 1102 36-37 0.789301835765329 1102 38-39 0.8012094466717272 1102 40-41 0.8142468405028609 1102 42-43 0.6811886366891216 1102 44-45 0.8315258600976136 1102 46-47 0.9333376415737646 1102 48-49 0.8000350737238833 1102 50-51 0.728847013159033 1102 52-53 0.8565253491171632 1102 54-55 0.9430273165098342 1102 56-57 0.9348959540703845 1102 58-59 0.9450502391248037 1102 60-61 1.082805023884589 1102 62-63 1.0889469454223573 1102 64-65 0.9129632074468788 1102 66-67 0.9229674681402074 1102 68-69 1.0368025833697487 1102 70-71 0.9188063529718562 1102 72-73 0.9370554206852297 1102 74-75 0.857309338211703 1102 76-77 0.8538847391668192 1102 78-79 0.6869274314299929 1102 80-81 0.8035892611253779 1102 82-83 0.7395941606500855 1102 84-85 0.7512408892820552 1102 86-87 0.6112478427498083 1102 88-89 0.5094335424193126 1102 90-91 0.5010099270949837 1102 92-93 0.844795687856525 1102 94-95 0.7393860194122226 1102 96-97 0.6271064764246361 1102 98-99 0.43714260649239023 1102 100-101 0.6675322206264553 1103 1 0.21974714746562896 1103 2 0.22453613616879053 1103 3 0.38342509421471505 1103 4 0.3048343777021927 1103 5 0.15940095757473927 1103 6 0.2659992295744473 1103 7 0.20689830534326603 1103 8 0.09972693414706413 1103 9 0.31001206005417004 1103 10-11 0.3153757053014843 1103 12-13 0.28711827931119416 1103 14-15 0.3919655846455896 1103 16-17 0.5200261956240055 1103 18-19 0.5323951333537593 1103 20-21 0.51015693281024 1103 22-23 0.4792055140799292 1103 24-25 0.5648793507886936 1103 26-27 0.6404799770908696 1103 28-29 0.5660393771341674 1103 30-31 0.5883464002356718 1103 32-33 0.5960339952106679 1103 34-35 0.568658266129475 1103 36-37 0.47464288986068226 1103 38-39 0.8481852469678159 1103 40-41 0.7291089609364363 1103 42-43 0.5574654607397136 1103 44-45 0.7611883452622763 1103 46-47 0.6781825453316586 1103 48-49 0.7697316890913086 1103 50-51 0.6914232090207477 1103 52-53 0.7293369680238584 1103 54-55 0.6888367741834642 1103 56-57 0.7769925558394277 1103 58-59 0.6365968535286513 1103 60-61 0.8049608782147573 1103 62-63 0.7467890643271957 1103 64-65 0.7620156623334893 1103 66-67 0.6627419948656765 1103 68-69 0.5447525473924983 1103 70-71 0.48852566117805907 1103 72-73 0.5678728154390704 1103 74-75 0.5371788847035468 1103 76-77 0.5915690562282201 1103 78-79 0.5849512706035895 1103 80-81 0.5223694474188605 1103 82-83 0.6129350986828257 1103 84-85 0.8574199890476137 1103 86-87 0.913296672628654 1103 88-89 1.0820833232753486 1103 90-91 1.0582708018476232 1103 92-93 0.8901555977954025 1103 94-95 0.8941718589942056 1103 96-97 0.8253316712355137 1103 98-99 0.7330513778159187 1103 100-101 0.9460345235814813 1104 1 0.2005273257306719 1104 2 0.27165415813543703 1104 3 0.3158133600915676 1104 4 0.27776420624449827 1104 5 0.29039272107697656 1104 6 0.309399083873231 1104 7 0.23713329831696228 1104 8 0.27631999383281425 1104 9 0.48632247530263584 1104 10-11 0.4169863805032179 1104 12-13 0.39867724252362535 1104 14-15 0.4257734421609456 1104 16-17 0.4874464480931593 1104 18-19 0.4836270252004624 1104 20-21 0.3685361461032741 1104 22-23 0.4647607909719582 1104 24-25 0.42384375749413294 1104 26-27 0.32308221674946935 1104 28-29 0.43940670741812227 1104 30-31 0.5280276887758362 1104 32-33 0.5314178940319252 1104 34-35 0.6018769493248683 1104 36-37 0.6450416573716993 1104 38-39 0.7319666314478468 1104 40-41 0.5374760324614059 1104 42-43 0.6080468722899255 1104 44-45 0.646084383861151 1104 46-47 0.7606987973868016 1104 48-49 0.7841127176782052 1104 50-51 0.5791755487991708 1104 52-53 0.6711102234906292 1104 54-55 0.799504014100826 1104 56-57 0.8286587647017214 1104 58-59 0.8400072970415735 1104 60-61 0.9323618630912875 1104 62-63 0.890356514834572 1104 64-65 0.9842717222700301 1104 66-67 0.9007783723990244 1104 68-69 0.8871245313574008 1104 70-71 0.818323948591626 1104 72-73 0.8009469916897736 1104 74-75 0.7683581489522133 1104 76-77 0.748599936128219 1104 78-79 0.6945615795618281 1104 80-81 0.8492576914007266 1104 82-83 1.0670193108015056 1104 84-85 1.0088218656474375 1104 86-87 1.001045827681022 1104 88-89 1.024447743515875 1104 90-91 1.0140986514807793 1104 92-93 0.97795849509993 1104 94-95 1.052542314290605 1104 96-97 1.0301155278591452 1104 98-99 1.2366521087105475 1104 100-101 1.67293033128772 1105 1 0.04723718845884051 1105 2 0.09267429886790168 1105 3 0.08289390585796141 1105 4 0.18678145393451473 1105 5 0.17975560112309097 1105 6 0.1434401109511967 1105 7 0.1547496436874809 1105 8 0.12599953589548818 1105 9 0.1296448280071374 1105 10-11 0.23766424972636457 1105 12-13 0.21989875465720132 1105 14-15 0.29585615810832167 1105 16-17 0.3033969789832227 1105 18-19 0.37553638531838374 1105 20-21 0.32794833320425454 1105 22-23 0.1329536093772603 1105 24-25 0.21402433714839475 1105 26-27 0.07564466283921689 1105 28-29 0.09176572648195247 1105 30-31 0.048805764718999 1105 32-33 0.22040265885846821 1105 34-35 0.14142906659846233 1105 36-37 0.2639018752838922 1105 38-39 0.32596815859745476 1105 40-41 0.1720524999080979 1105 42-43 0.08893784394584969 1105 44-45 -0.03338004405047457 1105 46-47 -0.03729754719339695 1105 48-49 -0.06794176955117592 1105 50-51 -0.09708050780994171 1105 52-53 -0.16370914696129546 1105 54-55 -0.039231920336327164 1105 56-57 -0.19022434448867642 1105 58-59 -0.3309934449791214 1105 60-61 -0.10356008064366762 1105 62-63 -0.020787306655769555 1105 64-65 -0.07048942410602166 1105 66-67 0.014806574773089665 1105 68-69 0.04794244650680213 1105 70-71 -0.10695867986196816 1105 72-73 -0.13488218948102215 1105 74-75 -0.06100603232435731 1105 76-77 -0.10959657841023684 1105 78-79 -0.07381899921430346 1105 80-81 0.06542719622665416 1105 82-83 0.19378799633122412 1105 84-85 0.19660997743032382 1105 86-87 0.32349492701799676 1105 88-89 0.3003779536566107 1105 90-91 0.4791901507337677 1105 92-93 0.37142214706309673 1105 94-95 0.35030046584084573 1105 96-97 0.2783134633731983 1105 98-99 0.34369078758642857 1105 100-101 0.3785151645286504 1106 1 0.23392666729271738 1106 2 0.259167210029414 1106 3 0.2740655044781093 1106 4 0.29542845456698785 1106 5 0.22948133771128454 1106 6 0.2785396496882484 1106 7 0.25135384331942134 1106 8 0.24670555470896005 1106 9 0.37759740897141114 1106 10-11 0.3326015548788632 1106 12-13 0.39119523328952255 1106 14-15 0.5054217075657021 1106 16-17 0.4180373861734523 1106 18-19 0.5113548857234349 1106 20-21 0.42744614118202406 1106 22-23 0.4560434764967809 1106 24-25 0.4310453492085742 1106 26-27 0.4325768896974935 1106 28-29 0.3596289507817758 1106 30-31 0.4912289821612674 1106 32-33 0.4080479860323649 1106 34-35 0.3852900915397086 1106 36-37 0.4089923555672783 1106 38-39 0.6262219188596561 1106 40-41 0.6557156211902111 1106 42-43 0.7094901856680949 1106 44-45 0.69887276171481 1106 46-47 0.7761079603155494 1106 48-49 0.7440103696879916 1106 50-51 0.5505168782894643 1106 52-53 0.7312857677942546 1106 54-55 0.7999488649304496 1106 56-57 0.8285788970940189 1106 58-59 0.7720916419333932 1106 60-61 0.9221341112117543 1106 62-63 0.8545699539689906 1106 64-65 0.701355697186905 1106 66-67 0.817114704291221 1106 68-69 0.748445294938719 1106 70-71 0.6900703712862395 1106 72-73 0.7159232252038699 1106 74-75 0.7666383527085721 1106 76-77 0.8040251195594514 1106 78-79 0.9089241831988559 1106 80-81 0.9246732672285596 1106 82-83 1.0472790919407693 1106 84-85 0.9791478194487269 1106 86-87 1.0054679091953105 1106 88-89 1.3118786374810547 1106 90-91 1.136992583774191 1106 92-93 0.8736255599301153 1106 94-95 0.9177689562452933 1106 96-97 1.0195503940632875 1106 98-99 1.0104159519398301 1106 100-101 1.352548176530135 1107 1 0.08901467339961044 1107 2 0.11697989826610566 1107 3 0.14863836551819531 1107 4 0.2801955674284997 1107 5 0.2608596553214042 1107 6 0.3020980169956644 1107 7 0.09294566567015039 1107 8 0.1681401336273538 1107 9 0.1639040798264375 1107 10-11 0.20445817805195077 1107 12-13 0.32309816479979503 1107 14-15 0.2740531290053525 1107 16-17 0.26347810350794987 1107 18-19 0.29726214294805686 1107 20-21 0.2349614224829395 1107 22-23 0.3096846989107789 1107 24-25 0.2537890140411463 1107 26-27 0.28669239820536774 1107 28-29 0.2919718283838719 1107 30-31 0.2980487376829686 1107 32-33 0.3243177723474773 1107 34-35 0.33433178030220034 1107 36-37 0.2785453838488081 1107 38-39 0.4909482065252888 1107 40-41 0.39347200308255736 1107 42-43 0.29464860566771733 1107 44-45 0.10583162239235833 1107 46-47 0.2461257608215206 1107 48-49 0.3684168787509421 1107 50-51 0.36086689861198096 1107 52-53 0.3964499186335573 1107 54-55 0.5236128981130221 1107 56-57 0.4348015306456716 1107 58-59 0.4618573080112114 1107 60-61 0.43481795688666125 1107 62-63 0.4236794895683147 1107 64-65 0.483486680087843 1107 66-67 0.21170046816485666 1107 68-69 0.4118004032110534 1107 70-71 0.2944558147282592 1107 72-73 0.49599127470300886 1107 74-75 0.5560775635865873 1107 76-77 0.4744342834986206 1107 78-79 0.47718112973090854 1107 80-81 0.4977313401126793 1107 82-83 0.5178840501867015 1107 84-85 0.4392123673935622 1107 86-87 0.4316656152831797 1107 88-89 0.468199674841852 1107 90-91 0.5288485989603515 1107 92-93 0.5331162920702148 1107 94-95 0.46851137583058033 1107 96-97 0.3201816308550498 1107 98-99 0.4782044670668846 1107 100-101 0.6193792811410432 1108 1 0.19822006875448395 1108 2 0.21686768823861158 1108 3 0.2736841537009056 1108 4 0.1404842300426239 1108 5 0.17969517489556353 1108 6 0.23227103836657648 1108 7 0.24346011618877128 1108 8 0.270851141801991 1108 9 0.2849326794731084 1108 10-11 0.2987829936241013 1108 12-13 0.2911041068307938 1108 14-15 0.38342747695844537 1108 16-17 0.3456363034179972 1108 18-19 0.3860402128428504 1108 20-21 0.30562620893612547 1108 22-23 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1874.0 4 1794.0 5 2111.0 6 2449.0 7 3223.0 8 4138.0 9 4900.0 10 6391.0 11 8454.0 12 11625.0 13 16450.0 14 15253.0 15 16607.0 16 21361.0 17 28732.0 18 24247.0 19 16556.0 20 16330.0 21 16748.0 22 18152.0 23 20208.0 24 22771.0 25 25885.0 26 30378.0 27 34649.0 28 40753.0 29 48131.0 30 57242.0 31 69659.0 32 86114.0 33 109118.0 34 146726.0 35 209304.0 36 305733.0 37 454005.0 38 388695.0 39 55019.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.40031458873205 18.768866139162366 14.690063456016103 21.14075581608948 2 14.77203618918305 21.484823650395686 40.72271822035614 23.020421940065127 3 20.214756507566303 26.604088667935105 30.579338775343196 22.601816049155396 4 12.216500623806253 16.861907154953304 38.76895128514318 32.15264093609726 5 12.87342112106266 38.80983540866481 33.927257535776555 14.389485934495974 6 29.0710307083132 36.7589304962291 18.468316474819595 15.701722320638108 7 26.516827316354913 35.0107961362273 21.013908013851776 17.458468533566013 8 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30.575269170028186 20.912314448888587 32-33 21.032734697808156 27.228563381265936 30.147428219343738 21.59127370158217 34-35 21.813203360109696 26.493051031719418 30.084237685257765 21.60950792291312 36-37 22.159697662610867 26.32582318278014 30.68263196824627 20.831847186362722 38-39 22.40200624942246 26.01807660847334 30.901571596272248 20.67834554583195 40-41 21.5258421444231 26.523820043966516 31.10788221387813 20.842455597732254 42-43 20.845804724278057 27.47594593062373 30.06498208847619 21.613267256622024 44-45 21.289852576356804 27.54553166543466 29.393328333987668 21.771287424220866 46-47 21.51583106343978 27.232839305228584 28.64451627092217 22.60681336040946 48-49 21.98799711643208 27.07964711599342 28.872076872890844 22.060278894683652 50-51 22.28530529757326 27.62535157925819 28.950188881911092 21.139154241257458 52-53 21.699863896031204 27.887491379832348 29.09995286322225 21.312691860914196 54-55 20.74092299266052 27.345345081926002 29.61272219827194 22.301009727141537 56-57 21.789070106899803 28.251721057738216 28.145796649092453 21.813412186269527 58-59 21.841461521121797 27.372253563358118 28.614889560813943 22.17139535470614 60-61 21.722806888525366 26.90016786056029 29.448582762610283 21.92844248830406 62-63 22.69477722371417 26.775234989740827 28.972694188907 21.557293597638004 64-65 21.983280013027116 27.200267732059952 29.38442810253676 21.432024152376176 66-67 21.301388419861862 27.377453403680967 29.17096281154273 22.15019536491444 68-69 21.031448258503513 28.144622522016178 29.04354779264619 21.78038142683412 70-71 21.508698117193273 27.84882841184479 28.144547916426333 22.497925554535602 72-73 21.396179553250448 27.346691322257723 28.202860359574732 23.0542687649171 74-75 21.402381987240684 27.95345680906673 28.416667362178003 22.22749384151458 76-77 21.99537321010346 26.902389195912868 28.637959574102556 22.464278019881117 78-79 21.38190711849673 27.0551730982804 29.20807814791428 22.354841635308592 80-81 21.37572154424587 28.246863539361705 28.50292297081992 21.8744919455725 82-83 21.37683608808944 27.86692880706153 28.250170077486874 22.50606502736215 84-85 21.49831405278349 27.284703973512624 28.121098953901896 23.09588301980199 86-87 21.854120166008553 27.359705379532016 28.49944707316755 22.286727381291882 88-89 22.3010271093804 26.61693186828986 28.595369567463862 22.486671454865874 90-91 21.5523266090084 26.929168836804628 29.04830053054791 22.470204023639063 92-93 21.462369282280083 27.77625958414715 28.54962527961453 22.211745853958238 94-95 21.428109775776154 27.772601083924076 28.018178969886907 22.781110170412862 96-97 21.136474912391527 27.14634338412656 27.968994218782367 23.748187484699546 98-99 21.6703292846585 27.12445218555467 27.78201142034892 23.423207109437914 100-101 22.448300628605992 26.519851441797194 28.505541193579027 22.52630673601779 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4585.0 1 2861.5 2 1299.5 3 1440.5 4 1216.5 5 1243.5 6 2165.5 7 4188.5 8 6391.5 9 7178.5 10 6041.5 11 4839.5 12 4758.0 13 4964.0 14 5246.5 15 5549.0 16 5743.0 17 5775.0 18 5866.5 19 6189.0 20 6657.5 21 7357.5 22 8316.0 23 9540.5 24 10976.0 25 12981.5 26 15819.0 27 18906.5 28 21552.5 29 24410.0 30 27856.5 31 31167.5 32 34374.5 33 38102.5 34 43255.0 35 47347.5 36 51226.0 37 56533.0 38 59558.5 39 60549.5 40 62358.5 41 65620.5 42 68597.5 43 71992.0 44 76316.0 45 78645.5 46 82338.5 47 97236.5 48 155999.0 49 157312.5 50 100443.0 51 83257.0 52 73319.5 53 68614.5 54 65204.5 55 59556.0 56 53057.0 57 46681.0 58 41308.0 59 36239.5 60 31401.5 61 26442.5 62 21061.5 63 15508.5 64 10932.0 65 8113.0 66 5769.5 67 4264.0 68 2984.0 69 1841.5 70 1294.0 71 909.0 72 601.5 73 446.5 74 345.0 75 256.0 76 179.0 77 101.0 78 66.0 79 40.5 80 27.5 81 27.0 82 20.5 83 12.5 84 13.0 85 12.0 86 8.5 87 7.5 88 6.0 89 5.5 90 5.5 91 2.5 92 2.0 93 3.0 94 2.5 95 1.0 96 0.5 97 1.0 98 1.5 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.32858558319576625 2 0.0895871539515216 3 0.1004449126291062 4 0.10517123111229008 5 0.11671026092258589 6 0.05829126129260127 7 0.06024991579914594 8 0.06199567307671837 9 0.06459301927017978 10-11 0.062165990859896166 12-13 0.059930569955687574 14-15 0.0582273921239096 16-17 0.05997314940148202 18-19 0.06797808521083851 20-21 0.06991544999448596 22-23 0.08524405048048776 24-25 0.09431347243470549 26-27 0.08865040614404371 28-29 0.08650014413142401 30-31 0.08060289088889276 32-33 0.07298117009168632 34-35 0.3217302924228599 36-37 0.4978175905058055 38-39 0.4707157732576384 40-41 0.4528536957468669 42-43 0.48406442951419837 44-45 0.49300611313103265 46-47 0.4869811215511181 48-49 0.5050986757366563 50-51 0.5013091050609504 52-53 0.49964850667496685 54-55 0.49109003807028245 56-57 0.4698641843417495 58-59 0.453811733277242 60-61 0.4132335214351317 62-63 0.484319906188965 64-65 0.5061205824357231 66-67 0.5175105841857384 68-69 0.5291773523334176 70-71 0.527325146441359 72-73 0.5150622660525574 74-75 0.5169357616675133 76-77 0.49006813137121563 78-79 0.4698641843417495 80-81 0.4770175312352169 82-83 0.4845966725866289 84-85 0.4870024112740154 86-87 0.4872153085029876 88-89 0.4920267858777604 90-91 0.5071211994118927 92-93 0.514168097690874 94-95 0.5205337248371442 96-97 0.5117623590034877 98-99 0.5155306399562964 100-101 0.49198420643196594 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2348551.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.40905967844366 #Duplication Level Percentage of deduplicated Percentage of total 1 71.62658563932182 33.95749073137764 2 14.860221635649756 14.09018268718838 3 5.5511861901573445 7.895295521259656 4 2.756102206997102 5.226568560456635 5 1.4685034567886683 3.4810184010447394 6 0.8981263304058369 2.554759487819516 7 0.5976431663331324 1.9833590377371053 8 0.4140162030366351 1.5702495102085186 9 0.3060044023986 1.3056642877663545 >10 1.3828107197727808 11.531799275728686 >50 0.08651228540964835 2.838106604868429 >100 0.04509302714690337 3.8392047607333257 >500 0.002518157803625439 0.8564912925972862 >1k 0.0035074340836210474 3.3741186246006305 >5k 7.194736581786765E-4 2.261464464324927 >10k+ 4.4967103636167274E-4 3.2342267522882233 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 20191 0.8597215900357283 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15924 0.6780350948308127 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 15896 0.6768428703485682 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12268 0.5223646410063055 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 11576 0.4928996645165466 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 8770 0.3734217396173215 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8053 0.34289227698270125 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 7066 0.3008663639835797 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 6770 0.2882628480284226 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 6639 0.2826849406293498 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 5459 0.23244119459189944 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 5270 0.22439367933674847 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5013 0.213450761767575 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 4225 0.17989815848154883 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 3902 0.1661449974899417 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3900 0.16605983859835277 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3284 0.1398308999889719 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 3096 0.13182596417961542 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 3071 0.13076147803475419 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 3062 0.13037826302260416 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2790 0.11879665376651391 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2746 0.11692315815155813 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2736 0.11649736369361363 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2593 0.11040850294500737 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 2550 0.10857758677584603 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 2518 0.10721504451042367 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 6.386916869167415E-5 0.0 12-13 0.0 0.0 0.0 1.49028060280573E-4 0.0 14-15 0.0 0.0 0.0 1.7031778317779772E-4 0.0 16-17 0.0 0.0 0.0 2.554766747666966E-4 0.0 18-19 0.0 0.0 0.0 3.193458434583707E-4 0.0 20-21 0.0 0.0 0.0 5.322430724306179E-4 0.0 22-23 0.0 0.0 0.0 0.001192224482244584 0.0 24-25 0.0 0.0 0.0 0.001937364783647449 0.0 26-27 0.0 0.0 0.0 0.003129589265892033 0.0 28-29 0.0 0.0 0.0 0.0076855899658981215 0.0 30-31 0.0 0.0 0.0 0.01788336723366876 0.0 32-33 0.0 0.0 0.0 0.03706540756406823 0.0 34-35 0.0 0.0 0.0 0.06878709468093305 0.0 36-37 0.0 0.0 0.0 0.11649736369361363 0.0 38-39 0.0 0.0 0.0 0.18117554185538232 0.0 40-41 0.0 0.0 0.0 0.2543696091760409 0.0 42-43 0.0 0.0 0.0 0.32890492903922464 0.0 44-45 0.0 0.0 0.0 0.42015268137672973 0.0 46-47 0.0 0.0 0.0 0.5281341559114534 0.0 48-49 0.0 0.0 0.0 0.6543609229690988 0.0 50-51 0.0 0.0 0.0 0.7873152424622671 0.0 52-53 0.0 0.0 0.0 0.9159903276530933 0.0 54-55 0.0 0.0 0.0 1.0579501999317877 0.0 56-57 0.0 0.0 0.0 1.2286299083988381 0.0 58-59 0.0 0.0 0.0 1.4314145189949037 0.0 60-61 0.0 0.0 0.0 1.6795249496391604 0.0 62-63 0.0 0.0 0.0 1.9162879579791965 0.0 64-65 0.0 0.0 0.0 2.140575188701459 0.0 66-67 0.0 0.0 0.0 2.4031413411929314 0.0 68-69 0.0 0.0 0.0 2.705817331622775 0.0 70-71 0.0 0.0 0.0 3.0565016471858604 0.0 72-73 0.0 0.0 0.0 3.4226423015723313 0.0 74-75 0.0 0.0 0.0 3.794041517514416 0.0 76-77 0.0 0.0 0.0 4.16844258438501 0.0 78-79 0.0 0.0 0.0 4.612120409563174 0.0 80-81 0.0 0.0 0.0 5.098590577764758 0.0 82-83 0.0 0.0 0.0 5.592086354522427 0.0 84-85 0.0 0.0 0.0 6.160500666155429 0.0 86-87 0.0 0.0 0.0 6.749459560384254 0.0 88-89 0.0 4.257944579444943E-5 0.0 7.331605743285967 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTTGCGT 690 0.0 26.08459 3 GAGTACT 11280 0.0 23.900267 12-13 GTACATG 15430 0.0 22.517561 1 TACATGG 15400 0.0 22.021242 2 CTTTGCG 805 0.0 21.769846 2 GTACTTT 12395 0.0 21.751 14-15 TTGCGTT 895 0.0 21.69753 4 AGTACTT 11615 0.0 21.458399 12-13 ACATGGG 15420 0.0 21.009768 3 GTCTACG 170 8.0031634E-5 19.544535 1 ACTTTTT 14345 0.0 19.028307 16-17 CATGGGG 4555 0.0 18.820871 4 TACACCG 180 1.2614086E-4 18.41938 5 CATGGGA 10375 0.0 18.260866 4 TACTTTT 13955 0.0 17.91167 14-15 ATGGGAG 3290 0.0 16.843775 5 GTATAGG 615 0.0 16.207663 1 TACCTGG 1835 0.0 15.745022 2 AGTACAT 11875 0.0 15.635147 2 CATGGGC 2445 0.0 14.916308 4 >>END_MODULE