##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938937_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2348551 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.17376160875365 33.0 31.0 34.0 30.0 34.0 2 32.401613164883365 34.0 31.0 34.0 31.0 34.0 3 32.48322859499325 34.0 31.0 34.0 30.0 34.0 4 36.02269399301952 37.0 35.0 37.0 35.0 37.0 5 35.96106152261543 37.0 35.0 37.0 35.0 37.0 6 36.018487569569494 37.0 35.0 37.0 35.0 37.0 7 35.9628123894265 37.0 35.0 37.0 35.0 37.0 8 35.943746165188664 37.0 35.0 37.0 35.0 37.0 9 37.598642737585855 39.0 37.0 39.0 35.0 39.0 10-11 37.51217942467505 39.0 37.0 39.0 35.0 39.0 12-13 37.41620322488207 39.0 37.0 39.0 34.0 39.0 14-15 38.655610416805935 40.0 38.0 41.0 34.0 41.0 16-17 38.765975488716236 40.0 38.0 41.0 34.5 41.0 18-19 38.86306876026963 40.0 38.0 41.0 35.0 41.0 20-21 38.849489536314096 40.0 38.0 41.0 34.5 41.0 22-23 38.78101050392348 40.0 38.0 41.0 34.0 41.0 24-25 38.69296664198478 40.0 38.0 41.0 34.0 41.0 26-27 38.48718592868539 40.0 38.0 41.0 34.0 41.0 28-29 38.315542008668324 40.0 38.0 41.0 34.0 41.0 30-31 38.1070745323393 40.0 37.5 41.0 33.5 41.0 32-33 37.9697947798451 40.0 37.0 41.0 33.0 41.0 34-35 37.85377984127234 40.0 37.0 41.0 33.0 41.0 36-37 37.688704013666296 40.0 37.0 41.0 33.0 41.0 38-39 37.507498453301636 40.0 36.5 41.0 32.5 41.0 40-41 37.30636549940793 40.0 36.0 41.0 32.0 41.0 42-43 37.12346548999787 40.0 36.0 41.0 31.0 41.0 44-45 36.838061851754546 40.0 36.0 41.0 31.0 41.0 46-47 36.43012351019841 39.5 36.0 41.0 30.0 41.0 48-49 36.02147238871969 39.0 35.0 41.0 29.0 41.0 50-51 35.738612659465346 39.0 35.0 41.0 28.0 41.0 52-53 35.42263676624438 39.0 35.0 40.0 27.0 41.0 54-55 35.142378428230856 38.0 35.0 40.0 26.5 41.0 56-57 35.04368225344052 38.0 34.5 40.0 26.5 41.0 58-59 34.786311006233205 38.0 34.0 40.0 26.0 41.0 60-61 34.40781507406056 38.0 34.0 40.0 25.5 41.0 62-63 33.96154288325014 37.0 33.5 40.0 24.0 41.0 64-65 33.5085725198218 36.5 33.0 39.5 22.5 41.0 66-67 33.05079962070229 36.0 32.5 39.0 22.0 40.0 68-69 32.575906165120536 35.5 32.0 39.0 20.5 40.0 70-71 32.02894210089541 35.0 31.0 38.0 19.0 40.0 72-73 31.49384641849379 35.0 31.0 37.0 17.5 39.0 74-75 30.95894894341234 34.5 30.5 37.0 13.5 39.0 76-77 29.03866660762317 32.5 28.0 35.0 8.0 37.0 78-79 29.96608419404135 34.0 29.5 35.5 7.5 37.0 80-81 29.96343617830739 34.0 30.0 35.0 6.5 37.0 82-83 29.551504736324652 34.0 29.5 35.0 2.0 37.0 84-85 29.099858806557748 34.0 29.0 35.0 2.0 36.0 86-87 28.39284030876911 34.0 28.5 35.0 2.0 36.0 88-89 27.658754057288938 33.0 26.5 35.0 2.0 35.0 90-91 27.036289184267233 32.5 25.5 35.0 2.0 35.0 92-93 26.65829867011617 32.0 25.0 34.0 2.0 35.0 94-95 26.109526256828147 32.0 24.5 34.0 2.0 35.0 96-97 25.208727636742825 32.0 21.5 34.0 2.0 35.0 98-99 23.965224302133528 31.5 4.5 34.0 2.0 35.0 100-101 18.388217671236433 24.0 2.0 31.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.15577513920540298 1101 2 0.15484544546036716 1101 3 0.18722872848012173 1101 4 0.12034130534318166 1101 5 0.1303741477743685 1101 6 0.1624790104272833 1101 7 0.12124296710550908 1101 8 0.16862214603067827 1101 9 0.22848900040962405 1101 10-11 0.316849352877135 1101 12-13 0.2650281075524745 1101 14-15 0.29847453971498084 1101 16-17 0.3092532974108835 1101 18-19 0.30325513791817826 1101 20-21 0.3575198599807621 1101 22-23 0.29257237804696956 1101 24-25 0.25145486086518076 1101 26-27 0.1500104560365898 1101 28-29 0.20208293913153597 1101 30-31 0.21326856995784027 1101 32-33 0.2764805582369263 1101 34-35 0.4083894751559498 1101 36-37 0.4262293102936141 1101 38-39 0.29484221568447566 1101 40-41 0.2526385437007477 1101 42-43 0.3079877818908088 1101 44-45 0.27100468349240714 1101 46-47 0.18912148906360216 1101 48-49 0.2912388534780419 1101 50-51 0.20009779242409564 1101 52-53 0.15904800028215504 1101 54-55 0.2688824451095684 1101 56-57 0.13678431638599164 1101 58-59 0.16871013492532683 1101 60-61 0.1255552363632333 1101 62-63 0.12584891907680174 1101 64-65 0.10083643682163057 1101 66-67 -0.07236908156455257 1101 68-69 0.06113556444763191 1101 70-71 0.07200514625899501 1101 72-73 0.09354360328905642 1101 74-75 0.18992459886513657 1101 76-77 0.05039106021290607 1101 78-79 0.28219046624467126 1101 80-81 0.4304733936797547 1101 82-83 0.1279551739682674 1101 84-85 0.43856458626784445 1101 86-87 0.4564495405267941 1101 88-89 0.4292507629569471 1101 90-91 0.6016104773684461 1101 92-93 0.4791324211484671 1101 94-95 0.30667205007140197 1101 96-97 0.6811560929841995 1101 98-99 0.9884016739882107 1101 100-101 1.2271917826704346 1102 1 0.2155868219471344 1102 2 0.18546991753689923 1102 3 0.17492131823529178 1102 4 0.13881944521916267 1102 5 0.1558742427187454 1102 6 0.13225919782783535 1102 7 0.17975554887225798 1102 8 0.19993218197858198 1102 9 0.15562672213748385 1102 10-11 0.24648443908008488 1102 12-13 0.2279343221529544 1102 14-15 0.3100318893918441 1102 16-17 0.3033634721740057 1102 18-19 0.2469367534131024 1102 20-21 0.2634355493751528 1102 22-23 0.24090594055638093 1102 24-25 0.28703477456402027 1102 26-27 0.3133884459659342 1102 28-29 0.3326874401497335 1102 30-31 0.3096927683326527 1102 32-33 0.30972123895535475 1102 34-35 0.3310628468599006 1102 36-37 0.4138443075762481 1102 38-39 0.4279375332897857 1102 40-41 0.4493328424546874 1102 42-43 0.4751560167766442 1102 44-45 0.399335430409991 1102 46-47 0.42384559380361964 1102 48-49 0.5371146662346362 1102 50-51 0.4127335792510536 1102 52-53 0.3888932736892201 1102 54-55 0.2356790873526151 1102 56-57 0.4534987826349024 1102 58-59 0.41841187862788587 1102 60-61 0.42131940762978815 1102 62-63 0.6769149152397347 1102 64-65 0.5487116471725315 1102 66-67 0.16963300066382203 1102 68-69 0.2538078230106251 1102 70-71 0.47518119932308167 1102 72-73 0.5675861383693679 1102 74-75 0.5987616284812347 1102 76-77 0.24262755683092152 1102 78-79 0.5363182548264689 1102 80-81 0.6399377109642153 1102 82-83 0.6947298807865856 1102 84-85 0.9431867403920293 1102 86-87 0.9910600394103746 1102 88-89 0.6726249551112673 1102 90-91 0.7331735891867339 1102 92-93 0.8351230838602071 1102 94-95 1.163482835752479 1102 96-97 1.3856790466055102 1102 98-99 1.5983731251966233 1102 100-101 1.5045331437144327 1103 1 0.06497588005790789 1103 2 0.09484688867953395 1103 3 0.14078418636326973 1103 4 0.09210013030037345 1103 5 0.09835742392560576 1103 6 0.09529672091314723 1103 7 0.08503806497107291 1103 8 0.1429365622594574 1103 9 0.14448795954247373 1103 10-11 0.1359206194768916 1103 12-13 0.11739334696286363 1103 14-15 0.14234566899024514 1103 16-17 0.20200789985866408 1103 18-19 0.18967706960143005 1103 20-21 0.1891702029639859 1103 22-23 0.26847925542363527 1103 24-25 0.12240548308945165 1103 26-27 0.15518363140302682 1103 28-29 0.09152590026739205 1103 30-31 0.2136665485763274 1103 32-33 0.24906715685362002 1103 34-35 0.34196006497337805 1103 36-37 0.2930006103498144 1103 38-39 0.2902598854978038 1103 40-41 0.41777528871672587 1103 42-43 0.4484460258429621 1103 44-45 0.334441381931768 1103 46-47 0.3756975827941247 1103 48-49 0.28347477170436974 1103 50-51 0.38608161962265086 1103 52-53 0.29174150645192043 1103 54-55 0.3850323955809145 1103 56-57 0.48548345111049684 1103 58-59 0.33759126833343345 1103 60-61 0.3393132697365857 1103 62-63 0.5964353471312336 1103 64-65 0.5174521534783523 1103 66-67 0.35596480937618225 1103 68-69 0.4289565877774848 1103 70-71 0.33276631316729066 1103 72-73 0.42992245706690113 1103 74-75 0.4580605063766541 1103 76-77 0.1512186152028363 1103 78-79 0.4544062012696344 1103 80-81 0.49487070754274853 1103 82-83 0.5399087460450396 1103 84-85 0.5846935881943232 1103 86-87 0.7652880552355974 1103 88-89 0.8561579442843161 1103 90-91 0.9440676892002209 1103 92-93 0.6817631254175538 1103 94-95 0.7924661814174669 1103 96-97 0.6883042919122815 1103 98-99 0.8157094579689144 1103 100-101 1.068044834532298 1104 1 0.04712549052610626 1104 2 0.0902011695484859 1104 3 0.09766697650176326 1104 4 0.10261370718563256 1104 5 0.08290752165688531 1104 6 0.11042018876134563 1104 7 0.10663448852371715 1104 8 0.07741878551671988 1104 9 0.12884697371052312 1104 10-11 0.0761512732613383 1104 12-13 0.13795075364043186 1104 14-15 0.09788689327855593 1104 16-17 0.09643025026611696 1104 18-19 0.17467302898024428 1104 20-21 0.25491489143806234 1104 22-23 0.17630930251777244 1104 24-25 0.16187220071172703 1104 26-27 0.1244470440758434 1104 28-29 0.2928443768536866 1104 30-31 0.2914873201558308 1104 32-33 0.20672423351395253 1104 34-35 0.2441830037589625 1104 36-37 0.23441359368175796 1104 38-39 0.10256208415305679 1104 40-41 0.3362303781080058 1104 42-43 0.29150073545564936 1104 44-45 0.423804795002674 1104 46-47 0.41630084952688406 1104 48-49 0.2549137503627179 1104 50-51 0.17791200111302885 1104 52-53 0.10280564285373117 1104 54-55 0.30994451704636816 1104 56-57 0.2789241097118449 1104 58-59 0.18647004571710823 1104 60-61 0.2063887756104137 1104 62-63 0.18398346635144947 1104 64-65 0.23474601213224133 1104 66-67 0.1408524880691715 1104 68-69 0.13221575680284303 1104 70-71 0.07831466328015324 1104 72-73 0.06293055025198768 1104 74-75 0.18672184658899837 1104 76-77 0.2566131430472929 1104 78-79 0.5343269015908945 1104 80-81 0.5217458145296412 1104 82-83 0.3374278444490919 1104 84-85 0.5656907259217832 1104 86-87 0.7693704759458733 1104 88-89 0.6272736732482045 1104 90-91 0.7535454339364662 1104 92-93 0.6606961625713659 1104 94-95 0.8145442947857511 1104 96-97 0.9559645931077476 1104 98-99 1.0760249847026984 1104 100-101 1.3007667004632957 1105 1 -0.11989961324303522 1105 2 -0.039300798462797104 1105 3 -0.0077406738552170395 1105 4 -0.028377091997384696 1105 5 -0.08066892223992284 1105 6 -0.09024311945345431 1105 7 -0.07735191481541648 1105 8 -0.02510460370167067 1105 9 -0.06905860405775854 1105 10-11 -0.021850340104769828 1105 12-13 0.043943837959766086 1105 14-15 0.08313509101449768 1105 16-17 -0.03294169448667361 1105 18-19 -0.031239406991026897 1105 20-21 0.05526263130852982 1105 22-23 0.025889256543621286 1105 24-25 -0.08854351448005104 1105 26-27 -0.15970706232409526 1105 28-29 0.006512206245609775 1105 30-31 -0.09588240212725907 1105 32-33 0.06400300138442105 1105 34-35 0.10764650803645992 1105 36-37 0.09280349661235476 1105 38-39 -0.05945457957985667 1105 40-41 0.16846205620434773 1105 42-43 0.20415713756938914 1105 44-45 0.2978752235026576 1105 46-47 0.36489476224305406 1105 48-49 0.36311066405163217 1105 50-51 0.36812854422267094 1105 52-53 0.38315225312842216 1105 54-55 0.6162092631607408 1105 56-57 0.5955105870534751 1105 58-59 0.6000057762681976 1105 60-61 0.44731977806478795 1105 62-63 0.3637862740555349 1105 64-65 0.23063885380802418 1105 66-67 0.21304443472990187 1105 68-69 0.3036000824042304 1105 70-71 0.34738287849089744 1105 72-73 0.35807905617198443 1105 74-75 0.3091971701104157 1105 76-77 0.13989516559051296 1105 78-79 0.27767954981441534 1105 80-81 0.2803838293788381 1105 82-83 0.19171308374577123 1105 84-85 0.264119989078079 1105 86-87 0.35462951061647985 1105 88-89 0.5114482959811788 1105 90-91 0.273232132766676 1105 92-93 0.6383256759151621 1105 94-95 0.640079326025262 1105 96-97 0.5596087967846408 1105 98-99 0.7179412301658559 1105 100-101 0.8661637094693262 1106 1 0.02968263991024145 1106 2 0.09304645307262405 1106 3 -0.015027979741354613 1106 4 0.02546394719954037 1106 5 0.022804797819105715 1106 6 -0.021603903067614283 1106 7 0.14077495450422362 1106 8 0.07931975203222663 1106 9 0.02578487312196387 1106 10-11 0.14154933694166516 1106 12-13 0.09844418276791345 1106 14-15 -0.03512908376655588 1106 16-17 -0.11626981515485824 1106 18-19 0.0033740410672677967 1106 20-21 0.051382863940119705 1106 22-23 -0.0649454880705349 1106 24-25 0.048129863010345275 1106 26-27 -0.12287533565000075 1106 28-29 -0.04533122382706978 1106 30-31 0.04824141836245843 1106 32-33 0.012775438046674026 1106 34-35 -0.06490757432982974 1106 36-37 -0.12887126706085184 1106 38-39 -0.07556499695602525 1106 40-41 0.098902900949561 1106 42-43 -0.024200648955364557 1106 44-45 -0.11528100277488562 1106 46-47 -0.03318817396242224 1106 48-49 -0.10365627884768003 1106 50-51 -0.1340899274547951 1106 52-53 -0.1261228157366716 1106 54-55 -0.12792494404695276 1106 56-57 -0.04901962825662309 1106 58-59 -0.10985052406780227 1106 60-61 -0.09746049072339957 1106 62-63 -0.02134705047419061 1106 64-65 -0.011419168174747085 1106 66-67 -0.014330860944049562 1106 68-69 0.05765611507953139 1106 70-71 0.08456107364226284 1106 72-73 0.19420676415153437 1106 74-75 0.039257760585677204 1106 76-77 -0.14064217137855906 1106 78-79 -0.049171158683311234 1106 80-81 -0.04822026522755962 1106 82-83 -0.0010443234514134758 1106 84-85 0.050852675114239076 1106 86-87 0.15448150953842443 1106 88-89 0.32415202431097256 1106 90-91 0.6907915166064882 1106 92-93 0.636394093882874 1106 94-95 0.40741182378767604 1106 96-97 0.41633058642000975 1106 98-99 0.6382662224626969 1106 100-101 0.9371526794698575 1107 1 0.04964938077383607 1107 2 0.0478420625063265 1107 3 0.062494293409152135 1107 4 0.08529098617938047 1107 5 0.06554290069725255 1107 6 0.09131671973617728 1107 7 0.03488896556282839 1107 8 0.13676832365702296 1107 9 0.05145543203083491 1107 10-11 0.09899925964851519 1107 12-13 0.08753841736304224 1107 14-15 0.10905614057499236 1107 16-17 0.10829550234380036 1107 18-19 0.11793059905064496 1107 20-21 0.10401482806438622 1107 22-23 0.018656654295234887 1107 24-25 0.03208942287285055 1107 26-27 0.059382817848678826 1107 28-29 0.0017261194727495877 1107 30-31 0.10252085815880463 1107 32-33 0.13202447286743535 1107 34-35 0.007369122769965486 1107 36-37 0.08394329580217885 1107 38-39 -0.011931284306406553 1107 40-41 -0.041969076145100814 1107 42-43 0.0957022532814662 1107 44-45 0.04088606752753776 1107 46-47 -0.01354175213433706 1107 48-49 -0.032862112819962874 1107 50-51 0.005150055116246222 1107 52-53 0.06291805111757043 1107 54-55 0.0822572606262213 1107 56-57 0.18692461483906442 1107 58-59 0.1150128759076523 1107 60-61 0.14267407704856794 1107 62-63 0.0612032182222535 1107 64-65 0.15427162318778187 1107 66-67 0.2611446354941549 1107 68-69 0.08008435854902274 1107 70-71 0.15763913128168383 1107 72-73 0.14032645651014874 1107 74-75 0.05459732691085861 1107 76-77 0.007702978293185225 1107 78-79 0.20852072030027102 1107 80-81 0.1614003959694763 1107 82-83 0.11984458720491276 1107 84-85 0.20246293973491447 1107 86-87 0.3145886728628504 1107 88-89 0.37749612126461685 1107 90-91 0.3299115906457821 1107 92-93 0.565429635222042 1107 94-95 0.7820924899161632 1107 96-97 0.7231396458755022 1107 98-99 0.6740150090221313 1107 100-101 0.6994002382334834 1108 1 0.013447156975104235 1108 2 0.054083165688545876 1108 3 0.02625265699746393 1108 4 0.0375443443658483 1108 5 -0.0033352772344699133 1108 6 0.05475398644828289 1108 7 0.09732202208051888 1108 8 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-0.8261724066914269 2316 54-55 -0.6897055966449841 2316 56-57 -0.8507592191167532 2316 58-59 -0.9197768962292159 2316 60-61 -0.9688047032346034 2316 62-63 -0.9249140123054786 2316 64-65 -0.700508532326829 2316 66-67 -0.8254630457942227 2316 68-69 -0.7352380002187502 2316 70-71 -0.8195108031731273 2316 72-73 -0.682611456380311 2316 74-75 -0.9328311099463633 2316 76-77 -0.8565921579314555 2316 78-79 -0.8139425930722943 2316 80-81 -0.9427350843602369 2316 82-83 -1.0011063693828 2316 84-85 -1.287139597915946 2316 86-87 -1.272118229944983 2316 88-89 -1.46852985269064 2316 90-91 -1.5113671781119606 2316 92-93 -1.4841787800938278 2316 94-95 -1.3211778271646573 2316 96-97 -1.321395766753671 2316 98-99 -1.3552198705485061 2316 100-101 -0.7339551174370946 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 8.0 4 21.0 5 83.0 6 193.0 7 354.0 8 785.0 9 1537.0 10 2607.0 11 4153.0 12 6135.0 13 8581.0 14 10292.0 15 14071.0 16 18659.0 17 27578.0 18 26931.0 19 17369.0 20 15257.0 21 16400.0 22 17223.0 23 19053.0 24 21638.0 25 25273.0 26 29505.0 27 35440.0 28 42710.0 29 51541.0 30 62189.0 31 75610.0 32 93812.0 33 119957.0 34 161877.0 35 230180.0 36 349971.0 37 507247.0 38 318919.0 39 15389.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.38283059768709 19.440337092148464 15.210099085154068 20.96673322501038 2 15.13669492380621 22.093069301028592 39.88101599667199 22.889219778493207 3 20.260407374589697 26.592439338128067 30.867117639770225 22.28003564751202 4 12.606539095808436 17.45778567295324 38.168087471807084 31.767587759431244 5 12.705834363401092 39.024700762299815 33.996749485108055 14.272715389191038 6 29.10066675154169 37.23006228095536 17.988495885335258 15.680775082167687 7 26.501106426898968 34.996259395686955 20.896331397529796 17.606302779884277 8 25.21524974335239 34.224549520108354 20.204032188357843 20.35616854818141 9 26.40487687940351 17.903592470421124 21.156364072996496 34.53516657717886 10-11 24.34418073101244 26.84506319002653 27.140160039104966 21.670596039856065 12-13 27.370259364178168 24.657267395939026 27.350502501329544 20.621970738553262 14-15 23.724728058441702 25.5258132911736 27.829511117280457 22.91994753310424 16-17 20.762075773256345 29.027033713314516 28.567057988450895 21.643832524978247 18-19 20.489163167863968 28.31042159570099 30.9558932639683 20.244521972466742 20-21 22.240074837633927 26.608725976144438 30.135751789081866 21.01544739713977 22-23 21.47647634647917 26.613218107675756 29.858921522249247 22.051384023595826 24-25 21.850522109831267 26.669435753495353 30.508637677407485 20.971404459265898 26-27 22.125519549185764 26.052601381149778 31.10220538157472 20.71967368808974 28-29 20.965991370849515 26.773295534139983 31.363530108564813 20.897182986445685 30-31 21.117276141757195 27.400341742631944 30.637529268046553 20.844852847564308 32-33 21.001374890304703 27.21586629372749 30.2881223358573 21.4946364801105 34-35 21.772004198880442 26.46580744343116 30.192952609289065 21.569235748399336 36-37 22.072835907855126 26.330608504812545 30.82733153419551 20.769224053136817 38-39 22.311563352677094 26.0444725498525 31.057625667902666 20.58633842956774 40-41 21.512676858797835 26.546033427680737 31.159361662535506 20.781928050985922 42-43 20.83013261614558 27.49717683969559 30.149755240943744 21.522935303215082 44-45 21.275003065771006 27.577483104195338 29.433637128530748 21.71387670150291 46-47 21.55191317791961 27.26547320937762 28.740078355496873 22.4425352572059 48-49 21.96135372666576 27.1216488963074 28.913978403052187 22.003018973974655 50-51 22.2482312943853 27.674518567816296 28.98297005436783 21.094280083430576 52-53 21.710429111396774 27.940504591980332 29.03447700305422 21.314589293568673 54-55 20.69899695599542 27.460442630370814 29.51615698360393 22.324403430029836 56-57 21.805913518590824 28.242839946843816 28.106947645590836 21.84429888897452 58-59 21.867611085208438 27.495757993813164 28.511093085689588 22.125537835288807 60-61 21.718170844129162 27.03868189047121 29.337262057087138 21.905885208312487 62-63 22.683941689134755 26.89115168898147 28.88187689587808 21.5430297260057 64-65 21.95480244271533 27.33573369599929 29.224039336879944 21.48542452440544 66-67 21.198128327672954 27.484087634256593 29.108111261373278 22.209672776697175 68-69 20.941326253622 28.20291292934411 28.95505419428187 21.90070662275202 70-71 21.37584437720202 28.03114091010604 28.022412121859848 22.570602590832088 72-73 21.281654092246665 27.421631465529174 28.117592507039447 23.179121935184714 74-75 21.269093155737302 28.114973871123087 28.160555167846045 22.455377805293562 76-77 21.844890562666837 27.023402134812713 28.558932163194626 22.572775139325817 78-79 21.197935340140024 27.173347458817936 29.090911800599027 22.537805400443013 80-81 21.11287272303025 28.399644290173885 28.366815432628776 22.120667554167085 82-83 21.10773834589924 27.994154693681338 28.133730116995544 22.764376843423882 84-85 21.144361170858367 27.433367943333558 28.00420940490741 23.418061480900665 86-87 21.43539495299074 27.423356508557045 28.375922343999815 22.765326194452392 88-89 21.795461450942433 26.746559225244887 28.473837025937282 22.984142297875398 90-91 21.057719062861842 27.070799981706205 28.859253604583024 23.012227350848928 92-93 20.848621128517113 27.97099147516916 28.42139685278284 22.75899054353088 94-95 20.724942836582883 28.00013199746224 27.8473981596999 23.427527006254977 96-97 20.399471844554366 27.48115753074981 27.76262895717402 24.356741667521803 98-99 20.91263932526907 27.31822728141735 27.619434280967287 24.149699112346294 100-101 21.306069893576527 26.792857015118315 28.583052804676264 23.318020286628897 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3143.0 1 2178.0 2 1283.0 3 1323.0 4 1158.0 5 1138.5 6 1908.0 7 3913.0 8 6087.5 9 6792.0 10 5655.0 11 4408.5 12 4286.5 13 4534.0 14 4695.0 15 4830.0 16 4988.0 17 5164.0 18 5414.5 19 5668.5 20 6167.5 21 7127.0 22 8204.0 23 9478.0 24 11067.5 25 12963.5 26 15777.0 27 18982.5 28 21680.5 29 24548.0 30 27972.0 31 31393.5 32 34717.0 33 38554.5 34 43333.0 35 47544.5 36 51834.0 37 56921.0 38 59778.5 39 60953.5 40 62696.0 41 65906.0 42 69572.5 43 72862.5 44 76612.0 45 79184.0 46 82482.5 47 97829.5 48 157396.0 49 158877.0 50 102562.0 51 84591.5 52 73368.5 53 68509.5 54 65205.5 55 59608.0 56 53194.5 57 46446.0 58 40928.0 59 36022.5 60 31243.0 61 26009.0 62 20487.0 63 15210.5 64 10730.0 65 7815.0 66 5458.0 67 3910.5 68 2697.0 69 1676.0 70 1122.5 71 749.5 72 509.5 73 373.0 74 255.5 75 185.5 76 130.5 77 68.5 78 31.5 79 16.5 80 11.5 81 7.0 82 2.5 83 1.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.5 89 1.0 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.39385987359865715 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 9.580375303751121E-4 16-17 0.002448318133180842 18-19 4.0450473504726955E-4 20-21 0.0 22-23 0.0 24-25 1.277383373833483E-4 26-27 2.1289722897224715E-5 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.013029310413101524 36-37 0.024461891608911195 38-39 0.010580992279920683 40-41 0.005875963519634021 42-43 0.005066954049539482 44-45 0.0016605983859835278 46-47 0.0010219066990667864 48-49 0.0030231406514059094 50-51 4.896636266361685E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 6.812711327111909E-4 60-61 0.0015328600486001793 62-63 0.0014902806028057299 64-65 7.238505785056403E-4 66-67 0.002682505085050314 68-69 0.002384448964489168 70-71 2.1289722897224715E-5 72-73 0.0 74-75 0.0 76-77 0.09207805153049689 78-79 0.003278617326172606 80-81 3.193458434583707E-4 82-83 0.0 84-85 2.1289722897224715E-5 86-87 0.09160967762675794 88-89 0.4326284589945034 90-91 0.38151183431826685 92-93 0.0 94-95 8.94168361683438E-4 96-97 0.0 98-99 0.0 100-101 3.832150121500448E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2348551.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.811475954014966 #Duplication Level Percentage of deduplicated Percentage of total 1 70.25536952112645 32.1850217145752 2 15.352188802065603 14.066128562946522 3 5.901850481142806 8.11117444203196 4 2.874528426259351 5.26745559514851 5 1.593118065394532 3.6491544972364225 6 0.9888151691610438 2.717944940699184 7 0.6445105826023642 2.0668186739897463 8 0.44537502972211584 1.6322629971706728 9 0.3063360279147796 1.2630335018999743 >10 1.4864410570914675 11.940211072668111 >50 0.09417837596537286 2.9650627541079535 >100 0.04984094104979656 4.1687415650769 >500 0.0026997261828447315 0.9183763968329983 >1k 0.0036306662458945745 3.681821896302991 >5k 6.516580441349236E-4 2.1214264469055113 >10k+ 4.6547003152494547E-4 3.245364942407348 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 20183 0.8593809544693729 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 16775 0.7142702032018892 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15516 0.6606626809466773 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13243 0.5638796006558938 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 10380 0.4419746473463851 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 9427 0.4013964355042748 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8702 0.3705263373032989 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 7170 0.3052946263462024 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 7000 0.298056120561146 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 6700 0.2852822868228112 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 5579 0.23755072808723335 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 5165 0.21992283752833128 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4836 0.20591419986195741 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 4562 0.1942474317142783 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 4081 0.17376671828714813 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3566 0.15183830370300666 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 3307 0.14081022724224426 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 3200 0.13625422654223818 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 3186 0.13565811430111588 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3071 0.13076147803475419 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3046 0.12969699188989298 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 2882 0.12271396277960325 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2821 0.12011661658614184 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2791 0.11883923321230835 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2730 0.11624188701884694 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 2598 0.11062140017397962 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 6.386916869167415E-5 0.0 12-13 0.0 0.0 0.0 1.49028060280573E-4 0.0 14-15 0.0 0.0 0.0 1.7031778317779772E-4 0.0 16-17 0.0 0.0 0.0 2.554766747666966E-4 0.0 18-19 0.0 0.0 0.0 3.193458434583707E-4 0.0 20-21 0.0 0.0 0.0 5.322430724306179E-4 0.0 22-23 0.0 0.0 0.0 0.001192224482244584 0.0 24-25 0.0 0.0 0.0 0.001937364783647449 0.0 26-27 0.0 0.0 0.0 0.0032360378803781564 0.0 28-29 0.0 0.0 0.0 0.007877197471973144 0.0 30-31 0.0 0.0 0.0 0.018181423354229904 0.0 32-33 0.0 0.0 0.0 0.037491202022012726 0.0 34-35 0.0 0.0 0.0 0.06987287054869151 0.0 36-37 0.0 0.0 0.0 0.11820054152539161 0.0 38-39 0.0 0.0 0.0 0.18377288804884373 0.0 40-41 0.0 0.0 0.0 0.25786112373118575 0.0 42-43 0.0 0.0 0.0 0.3331628736186696 0.0 44-45 0.0 0.0 0.0 0.42509189708888584 0.0 46-47 0.0 0.0 0.0 0.5335630352502458 0.0 48-49 0.0 0.0 0.0 0.6607691295611635 0.0 50-51 0.0 0.0 0.0 0.794404720187043 0.0 52-53 0.0 0.0 0.0 0.9233565717755331 0.0 54-55 0.0 0.0 0.0 1.0662531918617053 0.0 56-57 0.0 0.0 0.0 1.2377844892446448 0.0 58-59 0.0 0.0 0.0 1.44195293182903 0.0 60-61 0.0 0.0 0.0 1.6928097367270287 0.0 62-63 0.0 0.0 0.0 1.9322978295979096 0.0 64-65 0.0 0.0 0.0 2.1586501634412025 0.0 66-67 0.0 0.0 0.0 2.4227491759812754 0.0 68-69 0.0 0.0 0.0 2.727596718146636 0.0 70-71 0.0 0.0 0.0 3.080729351842902 0.0 72-73 0.0 0.0 0.0 3.4494247729770398 0.0 74-75 0.0 0.0 0.0 3.8242942137513727 0.0 76-77 0.0 0.0 0.0 4.201228757646737 0.0 78-79 0.0 0.0 0.0 4.647163293452005 0.0 80-81 0.0 0.0 0.0 5.135506957268546 0.0 82-83 0.0 0.0 0.0 5.63211103356921 0.0 84-85 0.0 0.0 0.0 6.200333737696138 0.0 86-87 0.0 0.0 0.0 6.790208090009542 0.0 88-89 0.0 0.0 0.0 7.374057450743033 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTTGCGT 740 0.0 28.214722 3 GACCGTA 70 0.008434785 27.115448 7 CTTTGCG 855 0.0 24.419762 2 GAGTACT 12055 0.0 22.654823 12-13 GTACATG 14900 0.0 22.316153 1 TTGCGTT 1015 0.0 21.505356 4 TACATGG 15200 0.0 21.44707 2 AGTACTT 12240 0.0 21.071754 12-13 GTACTTT 12900 0.0 21.060486 14-15 ACATGGG 15060 0.0 20.480627 3 CATGGGG 4740 0.0 19.921846 4 GTAAGCG 330 0.0 17.97502 94-95 ACTTTTT 15280 0.0 17.81061 16-17 GTATAGG 725 0.0 17.740911 1 TACTTTT 14605 0.0 17.61085 14-15 CATGGGA 10320 0.0 17.472649 4 ATGGGAT 4205 0.0 17.152699 5 ATGGGAG 3270 0.0 16.397799 5 GTGTAGC 1050 0.0 16.332903 1 GTATATG 1070 0.0 15.582402 1 >>END_MODULE