##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938936_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2359769 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.14762122902708 33.0 31.0 34.0 30.0 34.0 2 32.366964732564924 34.0 31.0 34.0 30.0 34.0 3 32.453193087967506 34.0 31.0 34.0 30.0 34.0 4 36.02218140843447 37.0 35.0 37.0 35.0 37.0 5 35.948520384834275 37.0 35.0 37.0 35.0 37.0 6 36.00463265684056 37.0 35.0 37.0 35.0 37.0 7 35.95142278756946 37.0 35.0 37.0 35.0 37.0 8 35.93136362076118 37.0 35.0 37.0 35.0 37.0 9 37.590390415333026 39.0 37.0 39.0 35.0 39.0 10-11 37.51494913273291 39.0 37.0 39.0 35.0 39.0 12-13 37.437610630532056 39.0 37.0 39.0 34.0 39.0 14-15 38.62080589244117 40.0 38.0 41.0 34.0 41.0 16-17 38.62617929975349 40.0 38.0 41.0 34.0 41.0 18-19 38.79336748639379 40.0 38.0 41.0 34.0 41.0 20-21 38.80629396351931 40.0 38.0 41.0 34.0 41.0 22-23 38.749049377290746 40.0 38.0 41.0 34.0 41.0 24-25 38.677762102985504 40.0 38.0 41.0 34.0 41.0 26-27 38.4756632958565 40.0 38.0 41.0 34.0 41.0 28-29 38.281674816475686 40.0 38.0 41.0 34.0 41.0 30-31 38.104749024162956 40.0 37.5 41.0 33.0 41.0 32-33 37.91233824158212 40.0 37.0 41.0 33.0 41.0 34-35 37.8649994978322 40.0 37.0 41.0 33.0 41.0 36-37 37.724527273644156 40.0 37.0 41.0 33.0 41.0 38-39 37.560179195505995 40.0 37.0 41.0 33.0 41.0 40-41 37.3788646261562 40.0 36.0 41.0 32.0 41.0 42-43 37.21248880716714 40.0 36.0 41.0 32.0 41.0 44-45 36.93045569290892 40.0 36.0 41.0 31.0 41.0 46-47 36.536754021262254 40.0 36.0 41.0 30.0 41.0 48-49 36.17932856987273 39.5 35.5 41.0 29.5 41.0 50-51 35.896013550478884 39.0 35.0 41.0 28.5 41.0 52-53 35.61690063730814 39.0 35.0 40.5 28.0 41.0 54-55 35.387378595108245 39.0 35.0 40.0 27.0 41.0 56-57 35.1521477314093 38.0 35.0 40.0 27.0 41.0 58-59 34.86967961694556 38.0 34.0 40.0 26.0 41.0 60-61 34.44782137573635 38.0 34.0 40.0 25.5 41.0 62-63 34.111090110938825 37.0 34.0 40.0 25.0 41.0 64-65 33.81526708758358 37.0 33.0 39.5 24.5 41.0 66-67 33.3667403461949 36.0 33.0 39.0 22.5 40.5 68-69 32.967697685663296 36.0 32.5 39.0 22.0 40.0 70-71 32.49140657411806 35.0 32.0 38.0 21.5 40.0 72-73 31.952512300992176 35.0 31.5 37.5 20.0 39.5 74-75 31.47873923252657 35.0 31.0 37.0 18.0 39.0 76-77 29.48245866438622 33.0 28.5 35.0 13.0 37.0 78-79 30.42841036559087 34.0 30.0 36.0 12.0 38.0 80-81 30.449510736008484 34.0 31.0 35.5 11.5 37.0 82-83 30.133648463048715 34.0 30.0 35.0 7.5 37.0 84-85 29.7396149792628 34.0 30.0 35.0 4.5 36.0 86-87 29.156571257610388 34.0 29.0 35.0 2.0 36.0 88-89 28.56273792053375 34.0 29.0 35.0 2.0 35.5 90-91 28.066103292313784 33.5 27.5 35.0 2.0 35.0 92-93 27.743492689326793 33.0 27.0 35.0 2.0 35.0 94-95 27.37542255195318 33.0 27.0 35.0 2.0 35.0 96-97 26.728753534773954 33.0 25.5 35.0 2.0 35.0 98-99 25.83322032792193 33.0 24.0 34.0 2.0 35.0 100-101 21.50563402604238 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.14785496801232512 1101 2 0.1626058090579221 1101 3 0.16071437601764416 1101 4 0.11890652386843925 1101 5 0.20576689803230863 1101 6 0.16624729719045206 1101 7 0.09396434041627089 1101 8 0.14063655473365344 1101 9 0.2811783343523615 1101 10-11 0.26109262599843674 1101 12-13 0.2351852547805109 1101 14-15 0.28915296877229935 1101 16-17 0.2918730383940229 1101 18-19 0.34235334741872947 1101 20-21 0.26078882193987596 1101 22-23 0.2716885613696789 1101 24-25 0.2851125196021087 1101 26-27 0.2232332027908157 1101 28-29 0.2862136168571965 1101 30-31 0.18885068345004186 1101 32-33 0.3020830183745673 1101 34-35 0.37014935483178135 1101 36-37 0.41485829250549244 1101 38-39 0.4149783900500026 1101 40-41 0.3910087011633365 1101 42-43 0.30709636707867816 1101 44-45 0.33896850919542487 1101 46-47 0.4230185423789763 1101 48-49 0.2500678712154425 1101 50-51 0.4418495279688841 1101 52-53 0.3553411472192707 1101 54-55 0.4612065296421548 1101 56-57 0.26898367847729787 1101 58-59 0.28713445869622234 1101 60-61 0.30968229582316553 1101 62-63 0.4086953207420194 1101 64-65 0.42125026831004675 1101 66-67 0.48602604799483373 1101 68-69 0.45235663807834214 1101 70-71 0.4061951861647941 1101 72-73 0.3181254686711483 1101 74-75 0.292532014087481 1101 76-77 0.051386002085216376 1101 78-79 0.38223354189542746 1101 80-81 0.6540019900408112 1101 82-83 0.5275915589987932 1101 84-85 0.2738329606434604 1101 86-87 0.4040939610228911 1101 88-89 0.3501052744166131 1101 90-91 0.23533575466323242 1101 92-93 0.4546932639599923 1101 94-95 0.5763129179617899 1101 96-97 0.48564662487908805 1101 98-99 0.40266726195709523 1101 100-101 1.1850129996766015 1102 1 0.10883711930924989 1102 2 0.07501223139171032 1102 3 0.09677876970915378 1102 4 0.10239572043269618 1102 5 0.033966664730868956 1102 6 0.09268151418157089 1102 7 0.030757723214499322 1102 8 0.05036634678789653 1102 9 0.1411453231329105 1102 10-11 0.16212118828608624 1102 12-13 0.16858953418964262 1102 14-15 0.15837234380281728 1102 16-17 0.2109721790428054 1102 18-19 0.1159982825648811 1102 20-21 0.09614448199287295 1102 22-23 0.12093479826724973 1102 24-25 0.061918762025790386 1102 26-27 0.011463009906819366 1102 28-29 0.11818391299214426 1102 30-31 0.10877603945287717 1102 32-33 0.23137471062025128 1102 34-35 0.23972896557362589 1102 36-37 0.25295082987163653 1102 38-39 0.2859242580903043 1102 40-41 0.15331026249637603 1102 42-43 0.07764688992944713 1102 44-45 0.15219626479606774 1102 46-47 0.22315389045621004 1102 48-49 0.14205086077712537 1102 50-51 0.29089788452329657 1102 52-53 0.23115684531099845 1102 54-55 0.11810227007300256 1102 56-57 0.08200254307001131 1102 58-59 -0.03187597338926906 1102 60-61 0.009268045529978508 1102 62-63 0.012203879456386346 1102 64-65 0.17388529697413446 1102 66-67 0.07212778445976653 1102 68-69 0.0276652223457603 1102 70-71 0.08906622738934544 1102 72-73 0.16779083794911287 1102 74-75 0.1227401883634549 1102 76-77 -0.11611654023572626 1102 78-79 0.0341446712883986 1102 80-81 0.25290206618059585 1102 82-83 0.2092436365346515 1102 84-85 0.31482556168448994 1102 86-87 0.2030021393499517 1102 88-89 0.36668141472156535 1102 90-91 0.42766506079399136 1102 92-93 0.31133451261092304 1102 94-95 0.4992923830720031 1102 96-97 0.3660385006704807 1102 98-99 0.38981895879402373 1102 100-101 1.0708739557554736 1103 1 -0.047966579625310146 1103 2 0.0014438238501455203 1103 3 0.0010651324612496182 1103 4 0.011282077214779918 1103 5 0.005370944403097155 1103 6 -0.028863729729280863 1103 7 0.0057909442499237684 1103 8 0.059901272878171596 1103 9 -0.1277981810352884 1103 10-11 0.06312620823928938 1103 12-13 -0.013543158500091579 1103 14-15 0.11099868409532121 1103 16-17 0.029282193319353667 1103 18-19 0.024248730572800525 1103 20-21 -0.0031037087344643055 1103 22-23 0.04616466930477969 1103 24-25 0.08644036367597607 1103 26-27 0.07842930921213309 1103 28-29 0.09201335398324773 1103 30-31 0.10808701819451016 1103 32-33 0.0672124622740995 1103 34-35 -0.0032162863658555807 1103 36-37 0.020785765328646733 1103 38-39 0.25382856915653207 1103 40-41 0.23827273893800083 1103 42-43 0.1369622123664911 1103 44-45 0.12484611456802241 1103 46-47 0.2320592895049245 1103 48-49 0.02573431448846719 1103 50-51 -0.012096897895645498 1103 52-53 0.15844997167033625 1103 54-55 0.05915358846690566 1103 56-57 0.09861954544668805 1103 58-59 0.04089255602278996 1103 60-61 0.05444628629216197 1103 62-63 0.18622446363195166 1103 64-65 0.04024512582017792 1103 66-67 0.17426288573889082 1103 68-69 0.07471269317249352 1103 70-71 0.05220708972611021 1103 72-73 0.17091387260706625 1103 74-75 0.06231248876190065 1103 76-77 0.059724587560531006 1103 78-79 0.19827531976507018 1103 80-81 0.1244656833054627 1103 82-83 0.15334686414737675 1103 84-85 0.04123224479955567 1103 86-87 -0.16110946686008987 1103 88-89 -0.16076816701761842 1103 90-91 0.1084163142383261 1103 92-93 0.23657910280590855 1103 94-95 0.1921961022840044 1103 96-97 -0.03753907803841372 1103 98-99 0.003385081263587608 1103 100-101 0.6384539307020276 1104 1 -0.0010477171536606988 1104 2 -0.043233015424966936 1104 3 0.014169518495251054 1104 4 0.008349216917757474 1104 5 0.07150859861831549 1104 6 -0.001976504064487017 1104 7 0.024956344821752907 1104 8 0.06463989162622852 1104 9 0.010322141146936303 1104 10-11 0.011665949541800558 1104 12-13 0.055863233174299864 1104 14-15 7.894597249133994E-4 1104 16-17 0.06364529957306075 1104 18-19 0.0021459020181495703 1104 20-21 0.032293115566758956 1104 22-23 -0.0729881853567349 1104 24-25 0.025658980832453437 1104 26-27 0.059130836727469216 1104 28-29 -0.024981116883850518 1104 30-31 -0.0363368118892069 1104 32-33 0.07263866999053192 1104 34-35 -0.030181568705678785 1104 36-37 0.021007348841777684 1104 38-39 0.1328408243590289 1104 40-41 0.18386875900171873 1104 42-43 0.11189972642480939 1104 44-45 0.11850302005630198 1104 46-47 0.30473340359719003 1104 48-49 0.23342069433964951 1104 50-51 0.15011583526948868 1104 52-53 0.24577458268620944 1104 54-55 0.34032550641584436 1104 56-57 0.2434907042259411 1104 58-59 0.32946544451360893 1104 60-61 0.2921710874861816 1104 62-63 0.1818398912850867 1104 64-65 0.01963400194210152 1104 66-67 0.21543894911644657 1104 68-69 0.22902637503581502 1104 70-71 -0.09413567591222716 1104 72-73 -0.03139999521182091 1104 74-75 0.007420172462044405 1104 76-77 0.10990802582206527 1104 78-79 0.2337255352380332 1104 80-81 0.30183003927800556 1104 82-83 0.3291806393620149 1104 84-85 0.16198754514215352 1104 86-87 -0.006015319750563464 1104 88-89 0.25646546787771385 1104 90-91 0.285883899909841 1104 92-93 0.2986491820684485 1104 94-95 0.4156667801773004 1104 96-97 0.424854149768521 1104 98-99 0.455724325114673 1104 100-101 0.8616299421094666 1105 1 -0.07561790583466177 1105 2 -0.027287989072306118 1105 3 -0.010209211953586816 1105 4 -0.028120550834408675 1105 5 0.04039198131601296 1105 6 -0.009187218278171372 1105 7 -0.01907245459238993 1105 8 0.015121407528589259 1105 9 -0.060342858147116374 1105 10-11 -0.07092737083112155 1105 12-13 -0.009083813264403773 1105 14-15 0.059018789140125705 1105 16-17 0.06148538230039691 1105 18-19 0.015076896375248339 1105 20-21 0.0841150330349123 1105 22-23 -0.06096502728887998 1105 24-25 -0.06571131862746427 1105 26-27 0.013478947822889609 1105 28-29 0.13282569338166184 1105 30-31 0.16868698851016006 1105 32-33 0.037444256102510565 1105 34-35 0.1281824331127055 1105 36-37 0.12739214730638793 1105 38-39 0.10029699764579902 1105 40-41 0.07993517670027472 1105 42-43 0.11887906941587545 1105 44-45 0.2215784148807316 1105 46-47 0.23931217221355183 1105 48-49 0.034240908232945344 1105 50-51 0.00864464787159136 1105 52-53 0.15689351176133215 1105 54-55 0.26343152059367725 1105 56-57 0.31068145030796046 1105 58-59 0.16116043169024863 1105 60-61 0.13008458839459536 1105 62-63 0.10975330685960927 1105 64-65 0.11660841108245279 1105 66-67 0.23857044709868802 1105 68-69 0.13202268085387914 1105 70-71 0.3192849410673517 1105 72-73 0.2004481275200405 1105 74-75 0.39005251369832905 1105 76-77 0.11422204214721177 1105 78-79 0.24748775931863776 1105 80-81 0.03867407374048781 1105 82-83 0.1440223760154069 1105 84-85 0.041047111304624906 1105 86-87 0.25112111518544467 1105 88-89 0.22728728759977557 1105 90-91 0.31389746644778427 1105 92-93 0.33893705984548816 1105 94-95 0.30877715414146323 1105 96-97 0.21224882058913863 1105 98-99 0.26681311580924216 1105 100-101 0.8190857585453983 1106 1 0.03937295360513815 1106 2 0.023969751254007576 1106 3 0.008981800201141255 1106 4 -0.021522084722242596 1106 5 -0.038122383313783814 1106 6 0.003576062601837293 1106 7 -0.022460083623066396 1106 8 0.07034277035601377 1106 9 0.048936334197598796 1106 10-11 0.14060022139503303 1106 12-13 0.07485223423546472 1106 14-15 0.058922064636881544 1106 16-17 0.06662938445369804 1106 18-19 -0.051682507196098015 1106 20-21 -0.053492419181090156 1106 22-23 -4.534398448257093E-4 1106 24-25 -0.014208170632912243 1106 26-27 -0.012827940286733508 1106 28-29 0.06541694850228907 1106 30-31 0.05520567488918715 1106 32-33 0.007859050900812292 1106 34-35 0.1336446229176005 1106 36-37 0.1635856614268434 1106 38-39 0.1881034449112562 1106 40-41 0.2860425056919169 1106 42-43 0.29921774146226454 1106 44-45 0.22502576324756518 1106 46-47 0.095000443714504 1106 48-49 0.146874131566598 1106 50-51 0.2349802694050851 1106 52-53 0.29202521419208693 1106 54-55 0.2648284419909217 1106 56-57 0.29037791708110916 1106 58-59 0.2901275086379016 1106 60-61 0.32258556226459234 1106 62-63 0.23533966366967718 1106 64-65 0.37951592693599423 1106 66-67 0.4400212767904961 1106 68-69 0.38656532538129795 1106 70-71 0.4994238230683834 1106 72-73 0.7112906414788469 1106 74-75 0.7164789729795835 1106 76-77 0.31851715901728994 1106 78-79 0.48397784477312555 1106 80-81 0.8079654068085702 1106 82-83 0.7260683207223053 1106 84-85 0.563067078573507 1106 86-87 0.6868207096996706 1106 88-89 0.7308330640325345 1106 90-91 0.9148385712573948 1106 92-93 0.9994642443523034 1106 94-95 1.1627247631533812 1106 96-97 1.02077462180306 1106 98-99 1.0748887032639018 1106 100-101 1.564202874693919 1107 1 -0.07921609048041489 1107 2 -0.05145582709605634 1107 3 -0.08456359482286047 1107 4 -0.09584006813560109 1107 5 -0.1381060149157065 1107 6 -0.06919783525935941 1107 7 -0.1286800748932535 1107 8 -0.03252715544057594 1107 9 -0.05206759421793805 1107 10-11 -0.045339324654726454 1107 12-13 -0.00578395083655181 1107 14-15 0.01976885643085069 1107 16-17 0.011025936177844642 1107 18-19 0.011534974253045505 1107 20-21 0.0020782922986057883 1107 22-23 -0.022569873716491884 1107 24-25 -0.14431511083370197 1107 26-27 -0.09954498524526656 1107 28-29 -0.06852292491543466 1107 30-31 -0.14153428582665128 1107 32-33 -0.2169690809193483 1107 34-35 -0.28409326446782046 1107 36-37 -0.13942885188612308 1107 38-39 -0.20722610550340903 1107 40-41 -0.17962477933645005 1107 42-43 -0.20257732619378288 1107 44-45 -0.2045873969444969 1107 46-47 -0.2824793685945366 1107 48-49 -0.1632906436407282 1107 50-51 -0.1864268672164826 1107 52-53 -0.15054080501349887 1107 54-55 -0.2001404580527293 1107 56-57 -0.12270316105071544 1107 58-59 -0.09373870698846076 1107 60-61 -0.23883139539582032 1107 62-63 -0.2617713140692999 1107 64-65 -0.2626299700522168 1107 66-67 -0.24132420553163314 1107 68-69 -0.04749458295567166 1107 70-71 -0.13602467103899585 1107 72-73 -0.25542149296026295 1107 74-75 -0.15149352811164007 1107 76-77 -0.32365329056803915 1107 78-79 -0.04348014300513725 1107 80-81 -0.11860323134071038 1107 82-83 -0.09934088922967987 1107 84-85 -0.3223514068040565 1107 86-87 -0.26168430994240666 1107 88-89 -0.0570870581848375 1107 90-91 0.1736938746183725 1107 92-93 0.21562530938940583 1107 94-95 0.26131980898489715 1107 96-97 0.15868110368869992 1107 98-99 -0.046368389708597135 1107 100-101 0.4580401728170109 1108 1 0.02784810678881655 1108 2 -0.0856116201389554 1108 3 -0.07254269021809989 1108 4 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6077.0 13 8529.0 14 9907.0 15 13464.0 16 18108.0 17 26483.0 18 25757.0 19 16132.0 20 13918.0 21 14273.0 22 15111.0 23 16649.0 24 18720.0 25 22522.0 26 26881.0 27 32493.0 28 39072.0 29 47298.0 30 57407.0 31 69936.0 32 86545.0 33 109513.0 34 149051.0 35 213598.0 36 329150.0 37 523903.0 38 408674.0 39 31029.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.37487207833295 19.44575291555021 15.158563294470678 21.02081171164616 2 15.130887811476462 22.04147948379693 39.9000919157765 22.927540788950104 3 20.215749931455154 26.604002340907094 30.861452964252013 22.31879476338574 4 12.58682523585995 17.519850459939086 38.14275888868784 31.750565415513126 5 12.702235406293038 39.019218137514564 34.02423985591694 14.254306600275454 6 29.132131153515452 37.209955720242114 17.951121486891303 15.70679163935114 7 26.441783072834674 35.01690207812714 20.873568556922308 17.66774629211588 8 25.193482921421545 34.252674732145394 20.194391908699536 20.35945043773352 9 26.372835646200965 17.952943699150214 21.149612525632804 34.524608129016016 10-11 24.359990321086514 26.859790089623182 27.14816577385329 21.63205381543702 12-13 27.318754505207927 24.65037891420728 27.377976403622554 20.652890176962234 14-15 23.682441258726886 25.541168102245443 27.865858581064344 22.910532057963326 16-17 20.712224470384122 29.076022410423676 28.54212104010437 21.669632079087837 18-19 20.52111880442535 28.338261075554428 30.904550403026736 20.236069716993484 20-21 22.22069194060944 26.584424153381114 30.122185688514424 21.072698217495017 22-23 21.47784380589795 26.601014760343066 29.80935845839148 22.111782975367504 24-25 21.835781383686285 26.673119275657914 30.49919292947742 20.99190641117838 26-27 22.146214311655083 26.063144316244514 31.06977844017783 20.720862931922575 28-29 20.98326149720587 26.739057933212955 31.324252500986326 20.95342806859485 30-31 21.12465245538864 27.395181477509027 30.594477679806793 20.885688387295538 32-33 20.939125821213857 27.27641985296018 30.25228316839487 21.532171157431087 34-35 21.785019436702207 26.462075228703853 30.20778815710907 21.545117177484872 36-37 22.09073300125636 26.394482778495533 30.77856418912834 20.736220031119768 38-39 22.27528766700622 26.073346159738975 31.06036541057884 20.591000762675964 40-41 21.500877437213763 26.55386481322093 31.164891662672538 20.78036608689277 42-43 20.823315505891195 27.507536280832962 30.149784839632478 21.51936337364337 44-45 21.264995194418407 27.596137058238224 29.40996865777648 21.728899089566887 46-47 21.502840325201873 27.309393096346458 28.67177451992042 22.515992058531253 48-49 21.977185844510657 27.105603401697543 28.92423942573894 21.99297132805286 50-51 22.211394847652908 27.68652907213638 28.999332347215894 21.102743732994824 52-53 21.72841918001296 27.964898259109262 29.013115266790944 21.293567294086838 54-55 20.719379735897878 27.452390467033 29.501086758915807 22.32714303815331 56-57 21.81039754314935 28.31493252093743 28.014394629304817 21.8602753066084 58-59 21.844820857009793 27.55005481482674 28.474151853385276 22.130972474778186 60-61 21.691128016208776 27.056598928088167 29.29501052884063 21.957262526862422 62-63 22.61865462254992 26.925961820839245 28.842590100980225 21.612793455630612 64-65 21.92142885747467 27.343718713707897 29.253087020096753 21.481765408720676 66-67 21.21887658314682 27.521905499274602 29.044849672961643 22.214368244616935 68-69 20.956667352478576 28.198057111110213 28.947802792137335 21.897472744273877 70-71 21.386415365232782 28.021344462106253 28.013335203572893 22.578904969088075 72-73 21.320858100941237 27.429803510428354 28.090673282003447 23.158665106626962 74-75 21.319438470460454 28.109213232312147 28.120040563292427 22.451307733934975 76-77 21.821182084475705 27.035055110606276 28.55515017329932 22.588612631618698 78-79 21.219349715183625 27.150467461604244 29.092121845979648 22.538060977232487 80-81 21.098082058031952 28.365594259438105 28.3704888063196 22.16583487621034 82-83 21.09638697686087 28.037998634612116 28.126714945403553 22.738899443123458 84-85 21.14732416605185 27.443194651679885 28.004097011190503 23.405384171077763 86-87 21.438467540465002 27.494175632659235 28.290223784299258 22.7771330425765 88-89 21.839909729644372 26.771387600254876 28.477470931779227 22.91123173832153 90-91 21.00531732819672 27.147926327046513 28.878194702231745 22.968561642525017 92-93 20.878039333511033 27.94332835120726 28.465370974870847 22.713261340410863 94-95 20.736521244240432 28.053063668520096 27.84723843732162 23.363176649917854 96-97 20.41227558860416 27.47789867301986 27.803843320274783 24.30598241810119 98-99 20.878314784201336 27.368356817976675 27.59501035906481 24.158318038757184 100-101 21.326954167046296 27.034090258987653 28.356319379260224 23.282636194705827 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3236.0 1 2169.0 2 1216.5 3 1319.5 4 1167.5 5 1186.5 6 2054.5 7 4076.0 8 6136.0 9 6832.0 10 5730.0 11 4454.5 12 4315.0 13 4497.5 14 4680.0 15 4833.0 16 4925.0 17 5123.5 18 5440.0 19 5698.0 20 6133.5 21 7066.5 22 8288.5 23 9547.5 24 10984.5 25 13049.5 26 15961.0 27 19147.0 28 21885.5 29 24631.0 30 28005.5 31 31377.5 32 34749.5 33 38662.0 34 43624.0 35 47759.5 36 51998.0 37 57156.0 38 59892.5 39 61110.5 40 63212.0 41 66504.5 42 69674.5 43 73302.5 44 77452.5 45 79439.0 46 82441.5 47 97755.0 48 159136.5 49 160340.0 50 102429.0 51 85230.5 52 74023.5 53 69066.0 54 65748.0 55 59569.5 56 53007.5 57 46575.5 58 41091.0 59 36242.5 60 31580.0 61 26345.0 62 20552.5 63 15078.0 64 10489.0 65 7720.0 66 5428.0 67 3950.0 68 2765.0 69 1687.5 70 1105.0 71 727.5 72 507.0 73 373.5 74 264.0 75 200.5 76 132.0 77 58.0 78 25.0 79 11.0 80 9.5 81 9.0 82 4.5 83 2.5 84 1.5 85 0.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4109724299285226 2 0.0 3 0.0 4 0.0 5 8.051635562633462E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0015043845393341467 16-17 0.002690941359090657 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0024578676980670567 36-37 0.007437168638116698 38-39 0.013285198678345211 40-41 0.00237311363951302 42-43 7.415980123478188E-4 44-45 5.509013806012368E-4 46-47 1.6950811710807288E-4 48-49 2.542621756621093E-4 50-51 4.025817781316731E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 2.542621756621093E-4 60-61 0.002542621756621093 62-63 0.0 64-65 4.025817781316731E-4 66-67 0.0012924993929490556 68-69 0.0015043845393341467 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.1274912925799093 78-79 1.2713108783105466E-4 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.14851029910131036 88-89 0.43995831795400314 90-91 0.34003328291879414 92-93 0.0 94-95 0.0 96-97 5.297128659627277E-4 98-99 0.0 100-101 1.6950811710807288E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2359769.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.77579088889605 #Duplication Level Percentage of deduplicated Percentage of total 1 70.1626286528178 32.11749817426654 2 15.42944723523106 14.125903003423842 3 5.892762574109113 8.092376020509946 4 2.853926246441048 5.225629242776697 5 1.627639377493964 3.7253239893348318 6 1.0015993226881128 2.7509400689898578 7 0.6476361166447231 2.0752238813337875 8 0.43640020046719935 1.5981251456367067 9 0.3184210026085719 1.311837590703835 >10 1.477544676666827 11.883653984446301 >50 0.09577882163879806 3.0293937482191504 >100 0.04870644046524639 4.100988361052785 >500 0.00278123434348717 0.9320599497956586 >1k 0.0036156046465331843 3.6666202095498237 >5k 6.489546801469819E-4 2.1259182800624816 >10k+ 4.635390572478442E-4 3.2385083498977565 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 20160 0.8543209102246871 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 16908 0.716510811015824 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15562 0.6594713296089575 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13176 0.5583597377539921 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 10506 0.4452130695843534 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 9412 0.3988525995552955 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8695 0.3684682695636734 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 7295 0.3091404285758479 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 7029 0.29786813878816104 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 6589 0.27922224590627304 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 5810 0.24621054009947582 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 5265 0.22311505914350088 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4743 0.20099424986089742 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 4571 0.19370540082525028 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 4172 0.17679696614372 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3587 0.15200640401666435 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 3331 0.14115788452174768 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 3279 0.13895427899934273 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 3197 0.13547936259862722 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3113 0.1319196921393577 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3107 0.1316654299636956 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2872 0.12170682808359631 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2735 0.11590117507264482 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 2733 0.11581642101409079 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 2715 0.11505363448710447 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2692 0.11407896281373305 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2423 0.10267954193821514 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2398 0.10162011620628968 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 4.237702927701822E-5 0.0 12-13 0.0 0.0 0.0 4.237702927701822E-5 0.0 14-15 0.0 0.0 0.0 1.0594257319254555E-4 0.0 16-17 0.0 0.0 0.0 2.330736610236002E-4 0.0 18-19 0.0 0.0 0.0 3.81393263493164E-4 0.0 20-21 0.0 0.0 0.0 5.085243513242186E-4 0.0 22-23 0.0 0.0 0.0 0.0010382372172869463 0.0 24-25 0.0 0.0 0.0 0.001991720376019856 0.0 26-27 0.0 0.0 0.0 0.0032842197689689117 0.0 28-29 0.0 0.0 0.0 0.00735241457956266 0.0 30-31 0.0 0.0 0.0 0.018434007735502925 0.0 32-33 0.0 0.0 0.0 0.03919875208124185 0.0 34-35 0.0 0.0 0.0 0.07333344916388002 0.0 36-37 0.0 0.0 0.0 0.12371973697425467 0.0 38-39 0.0 0.0 0.0 0.19071782026122047 0.0 40-41 0.0 0.0 0.0 0.26462335932034026 0.0 42-43 0.0 0.0 0.0 0.3429784864535469 0.0 44-45 0.0 0.0 0.0 0.437373319168105 0.0 46-47 0.0 0.0 0.0 0.553655887504243 0.0 48-49 0.0 0.0 0.0 0.6854060715264927 0.0 50-51 0.0 2.118851463850911E-5 0.0 0.8214787125349982 0.0 52-53 0.0 4.237702927701822E-5 0.0 0.9525932411180925 0.0 54-55 4.237702927701822E-5 4.237702927701822E-5 0.0 1.0997050982532612 0.0 56-57 4.237702927701822E-5 4.237702927701822E-5 0.0 1.2717982141472322 0.0 58-59 4.237702927701822E-5 4.237702927701822E-5 0.0 1.484043565281178 0.0 60-61 4.237702927701822E-5 4.237702927701822E-5 0.0 1.7356783651281122 0.0 62-63 4.237702927701822E-5 4.237702927701822E-5 0.0 1.983308535708368 0.0 64-65 4.237702927701822E-5 4.237702927701822E-5 0.0 2.218331540078711 0.0 66-67 4.237702927701822E-5 4.237702927701822E-5 0.0 2.4850101853189868 0.0 68-69 4.237702927701822E-5 4.237702927701822E-5 0.0 2.7953583592292297 0.0 70-71 4.237702927701822E-5 4.237702927701822E-5 0.0 3.1462401616429405 0.0 72-73 4.237702927701822E-5 4.237702927701822E-5 0.0 3.5187342489879305 0.0 74-75 4.237702927701822E-5 4.237702927701822E-5 0.0 3.9062721817262624 0.0 76-77 4.237702927701822E-5 4.237702927701822E-5 0.0 4.290695402812733 0.0 78-79 4.237702927701822E-5 4.237702927701822E-5 0.0 4.72989093423975 0.0 80-81 4.237702927701822E-5 4.237702927701822E-5 0.0 5.231401887218622 0.0 82-83 4.237702927701822E-5 4.237702927701822E-5 0.0 5.736557264715318 0.0 84-85 4.237702927701822E-5 4.237702927701822E-5 0.0 6.3066766280936815 0.0 86-87 4.237702927701822E-5 4.237702927701822E-5 0.0 6.906332780878128 0.0 88-89 4.237702927701822E-5 4.237702927701822E-5 0.0 7.498848404229397 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTTGCGT 750 0.0 27.204214 3 CTTTGCG 840 0.0 24.289476 2 GAGTACT 12050 0.0 22.486137 12-13 AGTACTT 12245 0.0 21.682388 12-13 TTGCGTT 950 0.0 21.47701 4 GTACATG 15075 0.0 21.2117 1 GTACTTT 12855 0.0 20.96728 14-15 CGCGGAA 115 8.495954E-8 20.77292 82-83 TACATGG 15265 0.0 20.5463 2 GTCTACG 165 6.169532E-5 20.217356 1 ACATGGG 15075 0.0 19.48329 3 CATGGGG 4195 0.0 19.228525 4 TACTTTT 14405 0.0 18.414686 14-15 TATCACG 155 9.6980424E-4 18.367437 2 ACTTTTT 14855 0.0 18.079697 16-17 ATCACGC 165 0.0014749614 17.25426 3 TCGCGGA 140 6.7210385E-7 17.063469 82-83 CATGGGA 10385 0.0 16.768282 4 ATGGGAG 3420 0.0 16.371367 5 ATGGGAT 3840 0.0 15.569272 5 >>END_MODULE