FastQCFastQC Report
Fri 27 May 2016
SRR938935_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938935_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2055034
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99260.48300904023972346No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77270.3760035113774274No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT72200.3513323867147697No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50520.2458353487095591No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT37410.18204078375345614No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT29720.14462047829865587No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC25620.1246694701888144No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA22780.11084974749809492No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT20880.10160415837402204No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGG650.005932404729.1422395
TACATGG84950.024.5281942
GTACATG88800.024.2627741
ACATGGG91600.021.7135143
GAGTACT77850.021.67691412-13
CATGGGA52500.020.1150844
AGTACTT81850.019.98139812-13
GTACTTT85450.019.5562514-15
GTATAGG2702.325578E-819.3342111
CATGGGG39450.018.2462184
TATTCCG2102.0087606E-518.0404345
CCCTATA4750.017.9454842
ATGGGAG22200.017.918545
GTATCAA216800.017.861931
AGAGTAC147150.017.29078510-11
TACTTTT96250.016.96841414-15
TTTGCGT4507.2759576E-1216.8377383
GGTATCA178250.016.3203121
TATCAAC240600.016.10032
ACTTTTT105550.015.96784916-17
Produced by FastQC (version 0.11.5)