##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938935_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2055034 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.880093468039945 33.0 31.0 34.0 30.0 34.0 2 31.942642311514067 33.0 31.0 34.0 30.0 34.0 3 31.98736468593707 34.0 31.0 34.0 30.0 34.0 4 35.531071505386286 37.0 35.0 37.0 33.0 37.0 5 35.575597289387915 37.0 35.0 37.0 33.0 37.0 6 35.67130373512068 37.0 35.0 37.0 33.0 37.0 7 35.597519554421 37.0 35.0 37.0 33.0 37.0 8 35.58833235849139 37.0 35.0 37.0 33.0 37.0 9 37.20941356688016 39.0 37.0 39.0 34.0 39.0 10-11 37.10404864347743 39.0 37.0 39.0 33.0 39.0 12-13 37.00144060876852 39.0 37.0 39.0 33.0 39.0 14-15 38.213562889957046 40.0 38.0 41.0 33.0 41.0 16-17 38.13945341050319 40.0 38.0 41.0 33.0 41.0 18-19 38.076598975978015 40.0 38.0 41.0 33.0 41.0 20-21 38.17611265799009 40.0 38.0 41.0 33.0 41.0 22-23 38.11185265061308 40.0 38.0 41.0 33.0 41.0 24-25 38.03208414070035 40.0 38.0 41.0 33.0 41.0 26-27 37.90600569139002 40.0 38.0 41.0 33.0 41.0 28-29 37.779567637323765 40.0 38.0 41.0 33.0 41.0 30-31 37.57778581765557 40.0 37.0 41.0 32.0 41.0 32-33 37.36156336099549 40.0 37.0 41.0 31.5 41.0 34-35 37.037835627050455 40.0 36.0 41.0 31.0 41.0 36-37 36.76007939528008 39.5 36.0 41.0 30.0 41.0 38-39 36.518761246772556 39.0 35.5 41.0 30.0 41.0 40-41 36.36110156814924 39.0 35.0 41.0 30.0 41.0 42-43 36.13432113531941 39.0 35.0 41.0 29.5 41.0 44-45 36.04363090829641 39.0 35.0 41.0 29.0 41.0 46-47 35.80593386776083 39.0 35.0 41.0 27.5 41.0 48-49 35.53939715839251 39.0 35.0 41.0 26.5 41.0 50-51 34.40254297495808 38.0 34.0 40.0 24.5 40.5 52-53 34.48456813853201 38.0 34.0 39.5 25.0 40.5 54-55 34.97387220844035 39.0 35.0 40.0 26.0 41.0 56-57 34.8664513579824 38.5 35.0 40.0 25.5 41.0 58-59 34.653027395167186 38.0 34.0 40.0 25.0 41.0 60-61 34.32898239153221 38.0 34.0 40.0 24.0 41.0 62-63 33.93996401032781 37.0 34.0 40.0 23.0 41.0 64-65 33.53984824581978 37.0 33.0 39.5 22.0 41.0 66-67 33.11293195149083 36.0 33.0 39.0 21.5 41.0 68-69 32.65897133575406 35.5 32.5 39.0 19.5 40.5 70-71 32.20237816016669 35.0 32.0 38.5 18.0 40.0 72-73 31.76696443951779 35.0 31.5 37.5 16.5 40.0 74-75 31.301923958435722 35.0 31.0 37.0 12.0 39.0 76-77 30.87354783424508 35.0 31.0 36.0 7.5 39.0 78-79 30.4906575754951 35.0 31.0 36.0 6.0 38.5 80-81 30.047979011539468 34.0 30.0 35.5 2.0 37.0 82-83 29.586310494132945 34.0 30.0 35.0 2.0 37.0 84-85 29.175810473208713 34.0 29.0 35.0 2.0 36.5 86-87 28.73223046431349 34.0 29.0 35.0 2.0 36.0 88-89 28.248642844838578 34.0 28.5 35.0 2.0 35.5 90-91 27.78740935673084 34.0 27.0 35.0 2.0 35.0 92-93 27.293763509508846 33.5 26.5 35.0 2.0 35.0 94-95 26.752031109947573 33.0 25.0 35.0 2.0 35.0 96-97 26.103682469487122 33.0 24.5 35.0 2.0 35.0 98-99 25.25382353771276 32.5 19.5 35.0 2.0 35.0 100-101 22.12403614733382 28.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.09930260981161965 1101 2 0.14408934290562314 1101 3 0.29004880172037417 1101 4 0.18630583520209854 1101 5 0.0900722030913883 1101 6 0.2061595436361756 1101 7 0.30994008074203805 1101 8 0.28203193228554113 1101 9 0.29518103283137975 1101 10-11 0.22512223161819378 1101 12-13 0.21538717715070987 1101 14-15 0.43319147492338317 1101 16-17 0.3870172366017002 1101 18-19 0.5070020789567451 1101 20-21 0.43273981711463705 1101 22-23 0.5592010896077255 1101 24-25 0.4464876355010432 1101 26-27 0.41011826460788825 1101 28-29 0.507002551564284 1101 30-31 0.4160417133635903 1101 32-33 0.36386886868201174 1101 34-35 0.3904893939942369 1101 36-37 0.3634688834675046 1101 38-39 0.6327238346092656 1101 40-41 0.5048072283085858 1101 42-43 0.5029868581425063 1101 44-45 0.5468513925937302 1101 46-47 0.3285877401328179 1101 48-49 0.49845187772782396 1101 50-51 0.3399269616312637 1101 52-53 0.38691264957274996 1101 54-55 0.4675660246130491 1101 56-57 0.4694071696106761 1101 58-59 0.34562394107013716 1101 60-61 0.3538823320130078 1101 62-63 0.3561604084806902 1101 64-65 0.24550429185809008 1101 66-67 0.22181837190351672 1101 68-69 0.27210362335567595 1101 70-71 0.28947708250374404 1101 72-73 0.10699051048350583 1101 74-75 -0.06629063953101522 1101 76-77 0.0423926653452078 1101 78-79 0.0018074817538895616 1101 80-81 0.1316538778426839 1101 82-83 0.05931691976729425 1101 84-85 -0.013634750315574706 1101 86-87 -0.08022046625921675 1101 88-89 0.029259143105537788 1101 90-91 -0.1278571194959639 1101 92-93 -0.2825925379136329 1101 94-95 -0.28982637247526455 1101 96-97 -0.5760106427477965 1101 98-99 -0.8440969507723715 1101 100-101 -0.6294392041077082 1102 1 0.1832604399499651 1102 2 0.29118020248627374 1102 3 0.31517847935825927 1102 4 0.19618137123621437 1102 5 0.23054634837018995 1102 6 0.2549948248091738 1102 7 0.27564773432397516 1102 8 0.3505269632465655 1102 9 0.3700265598107464 1102 10-11 0.36462863746105256 1102 12-13 0.3576404464883467 1102 14-15 0.42993376012784523 1102 16-17 0.4261651609846382 1102 18-19 0.3713110072870194 1102 20-21 0.392804307248511 1102 22-23 0.41513299750174326 1102 24-25 0.49308192326473943 1102 26-27 0.551430637725268 1102 28-29 0.29386824914473664 1102 30-31 0.29937777714086167 1102 32-33 0.28800246930367024 1102 34-35 0.2052299723022628 1102 36-37 0.24887630260024451 1102 38-39 0.36502613017466246 1102 40-41 0.29634032460986504 1102 42-43 0.232289908638748 1102 44-45 0.35324677489646916 1102 46-47 0.5749284840385798 1102 48-49 0.3779250090525821 1102 50-51 0.2926770905893932 1102 52-53 0.20030110466543505 1102 54-55 0.3131430928689838 1102 56-57 0.3273705831378848 1102 58-59 0.28878024939186275 1102 60-61 0.3893734909093425 1102 62-63 0.336350392031612 1102 64-65 0.28959800954937265 1102 66-67 0.3522326761827159 1102 68-69 0.305068199166449 1102 70-71 0.26120674862948334 1102 72-73 0.20855029502934386 1102 74-75 0.2932050511642963 1102 76-77 0.38238772840739443 1102 78-79 0.2493348618339013 1102 80-81 0.13285324378932373 1102 82-83 0.13990329736112628 1102 84-85 0.23384420128251193 1102 86-87 0.1207270162345786 1102 88-89 0.24940699664452737 1102 90-91 0.1338797306491557 1102 92-93 0.07491509441511823 1102 94-95 -0.099211314150768 1102 96-97 -0.029262214044482704 1102 98-99 -0.10991452317156813 1102 100-101 0.002856062409449578 1103 1 0.280514902493902 1103 2 0.21400571402907076 1103 3 0.35010974127704486 1103 4 0.3744279817686973 1103 5 0.10375182945255546 1103 6 0.2386598116420231 1103 7 0.2890254016472653 1103 8 0.16316664141907467 1103 9 0.33728944865709565 1103 10-11 0.33946747951531364 1103 12-13 0.3408560492384325 1103 14-15 0.4100418480869834 1103 16-17 0.42386196230185647 1103 18-19 0.46156956172423946 1103 20-21 0.44899893069496244 1103 22-23 0.5449654201843828 1103 24-25 0.4966671717901363 1103 26-27 0.5635929189908566 1103 28-29 0.5377203043822192 1103 30-31 0.5117667714131073 1103 32-33 0.517958002453959 1103 34-35 0.48029387129442114 1103 36-37 0.38826536479938056 1103 38-39 0.6708444891845389 1103 40-41 0.5495654483518848 1103 42-43 0.5301806103459583 1103 44-45 0.6603376403018615 1103 46-47 0.6337697299187539 1103 48-49 0.5881118529055058 1103 50-51 0.5688565904790792 1103 52-53 0.6276461401361857 1103 54-55 0.46795380420945776 1103 56-57 0.3949800113899684 1103 58-59 0.5524071690983519 1103 60-61 0.5766153617168968 1103 62-63 0.45998490719923524 1103 64-65 0.3528069262726916 1103 66-67 0.4125622787767398 1103 68-69 0.31615024642672296 1103 70-71 0.2865993351675016 1103 72-73 0.18840267474401529 1103 74-75 0.20856800810594578 1103 76-77 0.22233317357271076 1103 78-79 0.06602292640598506 1103 80-81 0.11401798782565109 1103 82-83 0.22159183629866774 1103 84-85 0.033332307621211754 1103 86-87 0.14449539228232666 1103 88-89 0.3057435086942526 1103 90-91 0.3105346906873727 1103 92-93 0.29108848649866204 1103 94-95 0.2948745274717943 1103 96-97 0.2225604440076303 1103 98-99 0.44327798443652355 1103 100-101 0.6331113440056981 1104 1 0.15237006880512283 1104 2 0.27341318802369585 1104 3 0.238218628891552 1104 4 0.2634683147300976 1104 5 0.32771455620644474 1104 6 0.26575495051694276 1104 7 0.3787996474038877 1104 8 0.32392143181768773 1104 9 0.3151221517032283 1104 10-11 0.3532578310091665 1104 12-13 0.43282690767555465 1104 14-15 0.5154528014984621 1104 16-17 0.3646909321260665 1104 18-19 0.5118531427402857 1104 20-21 0.45185472008249405 1104 22-23 0.5005695107175612 1104 24-25 0.4890976626811252 1104 26-27 0.4133270850400095 1104 28-29 0.3978555182798189 1104 30-31 0.4669291143453833 1104 32-33 0.41233182454072903 1104 34-35 0.4077025875836071 1104 36-37 0.4332781501268599 1104 38-39 0.45780433700070233 1104 40-41 0.41728064322926883 1104 42-43 0.31584773327386984 1104 44-45 0.28588820579666674 1104 46-47 0.4519524249318607 1104 48-49 0.24466163604133584 1104 50-51 0.00907612498237853 1104 52-53 0.344655958066177 1104 54-55 0.46494079897269813 1104 56-57 0.501931983257009 1104 58-59 0.4628551750199179 1104 60-61 0.4698864682546784 1104 62-63 0.4950517576931688 1104 64-65 0.37272442446560206 1104 66-67 0.3215527656332924 1104 68-69 0.4806173150111377 1104 70-71 0.47599606998307564 1104 72-73 0.5384895774925802 1104 74-75 0.43769956759622985 1104 76-77 0.3655550209815708 1104 78-79 0.2823613045171314 1104 80-81 0.28576093977436656 1104 82-83 0.5047892511795844 1104 84-85 0.418497359461103 1104 86-87 0.39409959690378216 1104 88-89 0.3074823903921491 1104 90-91 0.4698232813912746 1104 92-93 0.31546755278808547 1104 94-95 0.27654530894066554 1104 96-97 0.19948236987285028 1104 98-99 0.2755310156986397 1104 100-101 0.577700404373001 1105 1 0.2426426901397143 1105 2 0.2648284037663444 1105 3 0.36268320291576117 1105 4 0.2641101652891038 1105 5 0.3019089399479924 1105 6 0.348907760628002 1105 7 0.29218153131058955 1105 8 0.32115531751068715 1105 9 0.32457920796358053 1105 10-11 0.3413677409809779 1105 12-13 0.3361884975835139 1105 14-15 0.42098806892737883 1105 16-17 0.32854039716251293 1105 18-19 0.47839499779145456 1105 20-21 0.4505434053854671 1105 22-23 0.3532530485323093 1105 24-25 0.38832606793748425 1105 26-27 0.2754343842734315 1105 28-29 0.3384484042819764 1105 30-31 0.48601670356102744 1105 32-33 0.5422197583315551 1105 34-35 0.5169963405728808 1105 36-37 0.6451028395731839 1105 38-39 0.5819523457841953 1105 40-41 0.5281084692610776 1105 42-43 0.44956655569345116 1105 44-45 0.43530263937478253 1105 46-47 0.5948427871321798 1105 48-49 0.6023933060418827 1105 50-51 0.5394720693535646 1105 52-53 0.4597597930506012 1105 54-55 0.5023553488185115 1105 56-57 0.5532578314166798 1105 58-59 0.48586402457744526 1105 60-61 0.5229252831697053 1105 62-63 0.49410510078593717 1105 64-65 0.5151036051482549 1105 66-67 0.5278435088745752 1105 68-69 0.47088121626814683 1105 70-71 0.4084477065830967 1105 72-73 0.4270031213585277 1105 74-75 0.5017723194677437 1105 76-77 0.5092377211403836 1105 78-79 0.5256115681012936 1105 80-81 0.578165752200217 1105 82-83 0.6620709123279376 1105 84-85 0.7237045670015938 1105 86-87 0.7644615065479208 1105 88-89 0.6028337963064736 1105 90-91 0.860089957218424 1105 92-93 0.8174750203454302 1105 94-95 0.7436761655958009 1105 96-97 0.4166281563577847 1105 98-99 0.4103687826188924 1105 100-101 0.5952227396626242 1106 1 0.11388551802160052 1106 2 0.0869522412789081 1106 3 0.13579364399306826 1106 4 0.19052645983256866 1106 5 0.11958256563658409 1106 6 0.2079780251217258 1106 7 0.20187581778819919 1106 8 0.1784839182002287 1106 9 0.2477869735704914 1106 10-11 0.19341600885879018 1106 12-13 0.30535570494200925 1106 14-15 0.3790959019617475 1106 16-17 0.3874465457827938 1106 18-19 0.4517331655458676 1106 20-21 0.389968745385211 1106 22-23 0.41440211408959726 1106 24-25 0.41199692019502976 1106 26-27 0.3271576009445525 1106 28-29 0.3880005842242227 1106 30-31 0.3331465840999286 1106 32-33 0.36078011862314696 1106 34-35 0.2544249374457692 1106 36-37 0.24684577559343523 1106 38-39 0.4233896955036798 1106 40-41 0.5154391054963341 1106 42-43 0.4186714244643639 1106 44-45 0.5108601880688468 1106 46-47 0.2969788379190419 1106 48-49 0.17494778869522065 1106 50-51 0.1761583281477641 1106 52-53 0.15966119630493125 1106 54-55 0.059706213289331345 1106 56-57 0.10529019659358596 1106 58-59 0.1272174080360884 1106 60-61 0.22041583696536549 1106 62-63 0.2989185302987991 1106 64-65 0.28488274843795125 1106 66-67 0.2229804629348564 1106 68-69 0.26075819297387426 1106 70-71 0.2332800921154572 1106 72-73 0.18291701777469882 1106 74-75 0.16724819188991447 1106 76-77 0.10011656800373459 1106 78-79 0.20737816734050796 1106 80-81 0.34309584392699577 1106 82-83 0.3523931646592757 1106 84-85 0.3801935050828398 1106 86-87 0.3422696054754475 1106 88-89 0.29405932204976537 1106 90-91 0.38992533245855654 1106 92-93 0.29735746758027304 1106 94-95 0.3156874119858877 1106 96-97 0.3701591920688898 1106 98-99 0.5753637573654053 1106 100-101 0.8292875972380358 1107 1 0.08782035511433506 1107 2 0.0496750861268751 1107 3 0.15514863524478528 1107 4 0.16102818584571565 1107 5 0.1504569213371525 1107 6 0.15822628728657406 1107 7 0.11653037871882788 1107 8 0.10090317388661418 1107 9 0.22776049227290684 1107 10-11 0.17756033193062848 1107 12-13 0.1953046338680977 1107 14-15 0.16351449561569353 1107 16-17 0.2519024969815433 1107 18-19 0.20245903682784672 1107 20-21 0.21064780151610307 1107 22-23 0.23910538169256768 1107 24-25 0.21765729755352226 1107 26-27 0.30461539637652635 1107 28-29 0.30205695155764545 1107 30-31 0.33023837016887825 1107 32-33 0.4525023487267035 1107 34-35 0.5088132667596241 1107 36-37 0.43865785295665916 1107 38-39 0.5699140251958283 1107 40-41 0.4360803312020991 1107 42-43 0.4476306148932707 1107 44-45 0.2916967133443009 1107 46-47 0.31819934200945 1107 48-49 0.40986763548199434 1107 50-51 0.5550891457578189 1107 52-53 0.6209583070062124 1107 54-55 0.5475839264401401 1107 56-57 0.47308704721069006 1107 58-59 0.42489083689124385 1107 60-61 0.19963274716539559 1107 62-63 0.3367371334103595 1107 64-65 0.22685560510792868 1107 66-67 0.15705148955801462 1107 68-69 0.3389229444752715 1107 70-71 0.2331902448557983 1107 72-73 0.35055072468348314 1107 74-75 0.2695274750033576 1107 76-77 0.3692445767047339 1107 78-79 0.4588086302903349 1107 80-81 0.5279684026823723 1107 82-83 0.539131236253354 1107 84-85 0.6427035537895094 1107 86-87 0.7860701445590017 1107 88-89 0.8758085063312251 1107 90-91 1.049016456495064 1107 92-93 1.0858381031314117 1107 94-95 1.0200048092526401 1107 96-97 1.1263491953790528 1107 98-99 0.7892120573606753 1107 100-101 0.7540452460771156 1108 1 0.2988105054512964 1108 2 0.31595086536633943 1108 3 0.26823906547835463 1108 4 0.2709070380276657 1108 5 0.22119117502942487 1108 6 0.3161933534893038 1108 7 0.39066895147779235 1108 8 0.3485022766936581 1108 9 0.5082437141778442 1108 10-11 0.4831243387815789 1108 12-13 0.5493034436511479 1108 14-15 0.6107251305542221 1108 16-17 0.5386445251791869 1108 18-19 0.5415946778024008 1108 20-21 0.45346106872856495 1108 22-23 0.6172926998073933 1108 24-25 0.6456167395724037 1108 26-27 0.7096137412954349 1108 28-29 0.7009653444919195 1108 30-31 0.6186659813488902 1108 32-33 0.6232535650697457 1108 34-35 0.7625334669343431 1108 36-37 0.5807676720920725 1108 38-39 0.6769680426156199 1108 40-41 0.7033244487144046 1108 42-43 0.9308846948097482 1108 44-45 0.8316586850890033 1108 46-47 0.9148278929048672 1108 48-49 0.7276479683823922 1108 50-51 0.6952573977502254 1108 52-53 0.7201654896978198 1108 54-55 0.8062845565035133 1108 56-57 0.7230351811389255 1108 58-59 0.7612398669828337 1108 60-61 0.7496802889195138 1108 62-63 0.8004873824447216 1108 64-65 0.8822661759024157 1108 66-67 0.8371804262958236 1108 68-69 0.9225144498680535 1108 70-71 0.9343644515365597 1108 72-73 1.0372990849176809 1108 74-75 0.965865653116861 1108 76-77 0.9958746256673372 1108 78-79 0.8773150624554304 1108 80-81 0.9616192130028729 1108 82-83 0.9722542481611889 1108 84-85 1.0489505454286494 1108 86-87 1.0194887029859387 1108 88-89 1.0922523161992146 1108 90-91 1.253830039794547 1108 92-93 1.2965525782184528 1108 94-95 1.2892301334863738 1108 96-97 1.4923031990536906 1108 98-99 1.6918252064780788 1108 100-101 1.8476367360259118 1109 1 0.09872082904798773 1109 2 0.17012106380916947 1109 3 0.13803384164359755 1109 4 0.22833900066331836 1109 5 0.1628803836413084 1109 6 0.1491603648573232 1109 7 0.057728720186375426 1109 8 0.14659376628014087 1109 9 0.16319397448510387 1109 10-11 0.18917970720418253 1109 12-13 0.16260368270632597 1109 14-15 0.28064717350553536 1109 16-17 0.2912415783880036 1109 18-19 0.2513495049726302 1109 20-21 0.23616421035094248 1109 22-23 0.3208631719475221 1109 24-25 0.3352695567627535 1109 26-27 0.22872906515009106 1109 28-29 0.24735691343587973 1109 30-31 0.2603460994760525 1109 32-33 0.25963982713613376 1109 34-35 0.3478742004136208 1109 36-37 0.4202080913354038 1109 38-39 0.4011647017396385 1109 40-41 0.5330174114507926 1109 42-43 0.3347038039913883 1109 44-45 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70-71 -1.2483234353554487 2316 72-73 -1.360205529869983 2316 74-75 -1.3773766071715166 2316 76-77 -1.4393442983619416 2316 78-79 -1.3327190673225644 2316 80-81 -1.2784233572045913 2316 82-83 -1.1337352128428435 2316 84-85 -1.1985062527352959 2316 86-87 -1.2721240803033815 2316 88-89 -1.4731173440438674 2316 90-91 -1.5599777043150844 2316 92-93 -1.4007656983369294 2316 94-95 -1.3319443026798368 2316 96-97 -1.3357668746019975 2316 98-99 -1.4364618354976173 2316 100-101 -1.4598793634850686 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5355.0 3 1408.0 4 1408.0 5 1483.0 6 1885.0 7 2401.0 8 2916.0 9 3681.0 10 4687.0 11 6121.0 12 8797.0 13 12808.0 14 11618.0 15 11975.0 16 15216.0 17 20530.0 18 17498.0 19 12668.0 20 12905.0 21 13764.0 22 14762.0 23 16326.0 24 18656.0 25 21546.0 26 25031.0 27 29020.0 28 34580.0 29 41488.0 30 50239.0 31 60740.0 32 75472.0 33 95959.0 34 129358.0 35 186648.0 36 280757.0 37 412878.0 38 342545.0 39 49892.0 40 13.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.411813091995846 18.788482933385016 14.015561835572154 22.78414213904699 2 15.704757934273763 20.998137581310356 39.18029225946843 24.11681222494745 3 19.78493324767162 24.918937164795373 30.228193646777918 25.06793594075509 4 13.009063108579022 16.47736073860974 37.014853833871264 33.498722318939976 5 13.555509762054044 37.56465287307941 34.10271287523268 14.777124489633861 6 31.365927380089897 35.71191388752561 17.542086361759644 15.38007237062485 7 27.510600869298063 33.42415503439653 21.19287715066551 17.8723669456399 8 25.983975164231193 33.735804445587036 20.37956736059581 19.90065302958596 9 26.369726777712373 16.687433566614033 20.900071040813653 36.04276861485995 10-11 24.98387141537642 26.21336863687776 27.326180113321264 21.47657983442456 12-13 26.251718125635094 23.994917805064375 27.784970277582886 21.96839379171765 14-15 23.11998798369814 25.141360739070727 26.655643608561665 25.083007668669467 16-17 21.698571162866788 28.327015081033657 27.473612816590208 22.500800939509347 18-19 21.72062388508041 27.125313370465058 29.61068170568971 21.54338103876481 20-21 22.844146503815395 25.976954237156914 29.356614773771422 21.822284485256272 22-23 22.36013350002167 25.980052918839757 29.398081071117055 22.261732510021513 24-25 22.533102857760724 26.038844777721604 29.49244720054005 21.935605163977623 26-27 22.105032460416798 25.993317975716785 29.923560953990464 21.978088609875954 28-29 21.847898875227987 26.74327486661748 29.60215360114547 21.806672657009067 30-31 22.064848602021303 26.272980463410228 29.340873241782404 22.321297692786064 32-33 21.865104978380536 26.365980153287694 29.431003615863833 22.337911252467933 34-35 22.54858768824701 26.292427503803324 29.181658045479743 21.977326762469925 36-37 22.578212735118658 25.869146529881025 29.525247909302493 22.027392825697824 38-39 22.225044164482785 26.283116639945124 29.45491727241328 22.03692192315881 40-41 22.216981270413235 26.43311790577431 29.316559246163216 22.033341577649235 42-43 21.934721910861565 26.5704983951718 28.872948309839288 22.621831384127347 44-45 22.19414630592252 26.522939164582976 28.834764716079135 22.448149813415366 46-47 22.645586699749582 26.735893371902357 28.331266262444903 22.287253665903158 48-49 22.420055349588054 26.744394741642424 28.28318468951561 22.55236521925391 50-51 22.156597856850315 27.118802379103986 28.527755194356658 22.19684456968904 52-53 22.483625739430483 27.14575738733626 27.97655534997652 22.394061523256745 54-55 22.033768602436563 27.075319239448536 28.076837506326115 22.814074651788793 56-57 22.308710493927503 27.195284265280854 27.805601829388277 22.690403411403366 58-59 22.4752496057827 26.79832009173801 27.979530028457944 22.746900274021346 60-61 22.13042924860301 26.77813258791445 28.612436661260233 22.479001502222307 62-63 22.064512816877134 26.836050805462897 28.555960694339692 22.543475683320274 64-65 22.004173078338503 27.21369872995713 28.179209090884637 22.602919100819722 66-67 22.08609511214061 27.01024564506284 27.89651741974864 23.00714182304791 68-69 21.93812974590898 27.024132397638052 28.164605800492243 22.873132055960728 70-71 22.349590242919376 26.889152809993433 27.82816518634971 22.933091760737476 72-73 22.227145698039642 26.715179206368305 28.046474025078428 23.01120107051363 74-75 21.947696243021234 27.553133994752017 27.525524614899282 22.973645147327463 76-77 22.219899604225894 26.940168382470155 27.701498113056246 23.138433900247712 78-79 22.2706156555663 26.94183947465232 27.84856043930526 22.938984430476133 80-81 22.358546375472407 27.029217207644585 27.705647388980964 22.90658902790204 82-83 22.191214849312722 26.85994731598795 27.978437448431396 22.97040038626793 84-85 22.202362441887527 26.96565374452613 27.752066818829906 23.07991699475643 86-87 22.207529059945657 26.886531137524578 27.753501055414002 23.152438747115763 88-89 22.31410232431231 26.805909763373286 27.44498746122662 23.43500045108778 90-91 22.191808691118887 26.669411872297076 27.6411804319004 23.49759900468364 92-93 22.381412933036135 26.780043992913207 27.57037582272739 23.26816725132327 94-95 22.407126393211097 26.604483953998816 27.564297112392318 23.424092540397773 96-97 22.143161065980664 26.51824971022502 27.721048178452477 23.617541045341834 98-99 22.313390934852507 26.72528222820649 27.30345755841422 23.65786927852679 100-101 22.71078946725019 25.809051123444416 27.88276570255006 23.597393706755337 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2936.0 1 1833.0 2 847.0 3 982.5 4 869.0 5 887.0 6 1437.5 7 2591.0 8 3901.5 9 4404.5 10 3924.0 11 3391.5 12 3485.5 13 3719.0 14 3879.5 15 4242.0 16 4504.0 17 4590.5 18 4733.0 19 5008.0 20 5334.0 21 5795.0 22 6633.5 23 7849.5 24 9172.5 25 10747.0 26 13022.0 27 15948.0 28 18278.0 29 20348.5 30 22985.5 31 26583.0 32 30221.5 33 32299.0 34 34282.0 35 37284.0 36 40393.5 37 43080.5 38 45853.0 39 48343.5 40 51052.0 41 54615.0 42 56875.0 43 59271.0 44 63068.0 45 66086.5 46 69926.0 47 77982.5 48 93403.5 49 95475.0 50 83107.0 51 77232.0 52 75018.0 53 74112.5 54 72341.5 55 67929.5 56 62428.0 57 56564.5 58 51623.5 59 46572.5 60 39419.5 61 32237.5 62 25835.5 63 19313.0 64 13578.5 65 9663.0 66 7011.5 67 5313.5 68 3965.0 69 2835.0 70 2043.5 71 1273.5 72 842.0 73 589.5 74 381.5 75 267.0 76 197.5 77 124.0 78 69.0 79 49.0 80 35.0 81 25.0 82 24.5 83 20.0 84 12.5 85 11.5 86 10.0 87 10.5 88 9.0 89 5.5 90 5.5 91 4.0 92 5.0 93 3.0 94 2.0 95 1.5 96 1.5 97 2.0 98 1.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.31926479075285374 2 0.08710318174784457 3 0.09717600779354502 4 0.10199344633714089 5 0.11391539020765593 6 0.056544076643014175 7 0.05805256750009975 8 0.05946373636640562 9 0.06252937907596663 10-11 0.0597070413433549 12-13 0.05824721148165919 14-15 0.05632510216375983 16-17 0.058198550486269325 18-19 0.06591131825556171 20-21 0.06720083463339292 22-23 0.08277235315814725 24-25 0.09187195929605058 26-27 0.08583799586770828 28-29 0.08413486102906327 30-31 0.07810089760072096 32-33 0.07029080784064887 34-35 0.31607749555481807 36-37 0.49062448601823616 38-39 0.46498014144778144 40-41 0.44670793767889 42-43 0.47685342432290656 44-45 0.4858070474746404 46-47 0.48025969400019664 48-49 0.4969747459166125 50-51 0.4937387897231871 52-53 0.4930575357877291 54-55 0.4836416331797917 56-57 0.4642988875123234 58-59 0.44807044554980596 60-61 0.40872803077710634 62-63 0.4759775264058892 64-65 0.49928614319763076 66-67 0.5088966897871275 68-69 0.521037608136897 70-71 0.5183369228927599 72-73 0.5064636400176348 74-75 0.5079721308747203 76-77 0.48310636223050324 78-79 0.46381227755842486 80-81 0.4702598594475809 82-83 0.4779239662214834 84-85 0.47989473653477266 86-87 0.4800407195209422 88-89 0.48551508150230116 90-91 0.49911582971376633 92-93 0.5054174286167528 94-95 0.5119866629943836 96-97 0.5033493363126839 98-99 0.5066582839991941 100-101 0.4858313779723352 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2055034.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.563024556980935 #Duplication Level Percentage of deduplicated Percentage of total 1 70.09232414689728 32.63710610507846 2 14.489472009301121 13.493472819735523 3 5.912299677116204 8.258836651613768 4 2.9612813508033984 5.515448650303532 5 1.6980619536850272 3.9533450224355478 6 1.087438327722954 3.0380650534779723 7 0.7494301440860347 2.442701394197386 8 0.5424354305407872 2.0205947418277765 9 0.4032116158919109 1.6897277135191513 >10 1.912208439029139 15.528834629583363 >50 0.09816038628920722 3.110042023969112 >100 0.04791218089963195 4.064682082140279 >500 0.0033537965017038625 1.0630790021185947 >1k 0.0019913166728866656 1.723696907539058 >5k 4.192245627129822E-4 1.4603672024604715 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9926 0.48300904023972346 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7727 0.3760035113774274 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7220 0.3513323867147697 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5052 0.2458353487095591 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3741 0.18204078375345614 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2972 0.14462047829865587 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2562 0.1246694701888144 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2278 0.11084974749809492 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2088 0.10160415837402204 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 4.8660995389857295E-5 0.0 16-17 0.0 0.0 0.0 1.2165248847464323E-4 0.0 18-19 2.4330497694928648E-5 0.0 0.0 1.4598298616957188E-4 0.0 20-21 4.8660995389857295E-5 0.0 0.0 2.189744792543578E-4 0.0 22-23 4.8660995389857295E-5 0.0 0.0 5.839319446782875E-4 0.0 24-25 9.732199077971459E-5 0.0 0.0 0.001313846875526147 0.0 26-27 9.732199077971459E-5 0.0 0.0 0.002773676737221866 0.0 28-29 1.4598298616957188E-4 0.0 0.0 0.00756678478312281 0.0 30-31 1.4598298616957188E-4 0.0 0.0 0.02087556702224878 0.0 32-33 1.4598298616957188E-4 0.0 0.0 0.04749313150050072 0.0 34-35 1.4598298616957188E-4 0.0 0.0 0.09077708689977879 0.0 36-37 1.9464398155942918E-4 0.0 0.0 0.15578817674062814 0.0 38-39 1.9464398155942918E-4 0.0 0.0 0.25595683575064937 0.0 40-41 1.9464398155942918E-4 0.0 0.0 0.3653224228893537 0.0 42-43 1.9464398155942918E-4 0.0 0.0 0.47760766975144936 0.0 44-45 2.4330497694928648E-4 0.0 0.0 0.6126176014606084 0.0 46-47 3.4062696772900106E-4 0.0 0.0 0.774756038099613 0.0 48-49 3.4062696772900106E-4 0.0 0.0 0.9486461051252679 0.0 50-51 3.4062696772900106E-4 0.0 0.0 1.1405164099474754 0.0 52-53 3.4062696772900106E-4 0.0 0.0 1.328518165636189 0.0 54-55 3.4062696772900106E-4 0.0 0.0 1.5351327520615232 0.0 56-57 3.4062696772900106E-4 0.0 0.0 1.787877962116442 0.0 58-59 3.4062696772900106E-4 0.0 0.0 2.059260333405676 0.0 60-61 3.4062696772900106E-4 0.0 0.0 2.359206708988756 0.0 62-63 3.4062696772900106E-4 0.0 0.0 2.6577662462032254 0.0 64-65 3.4062696772900106E-4 0.0 0.0 2.9726272168732972 0.0 66-67 3.8928796311885836E-4 0.0 0.0 3.340990951974517 0.0 68-69 3.8928796311885836E-4 0.0 0.0 3.763903662907767 0.0 70-71 4.13618460813787E-4 0.0 0.0 4.213263624835404 0.0 72-73 4.622794562036443E-4 0.0 0.0 4.661772019343719 0.0 74-75 4.8660995389857297E-4 0.0 0.0 5.135559800957065 0.0 76-77 4.8660995389857297E-4 0.0 0.0 5.6489089718223635 0.0 78-79 4.8660995389857297E-4 0.0 0.0 6.240991633228452 0.0 80-81 5.839319446782875E-4 0.0 0.0 6.880689078623517 0.0 82-83 5.839319446782875E-4 0.0 0.0 7.527612681833975 0.0 84-85 5.839319446782875E-4 0.0 0.0 8.197406952877664 0.0 86-87 5.839319446782875E-4 0.0 0.0 8.895886880703678 0.0 88-89 5.839319446782875E-4 0.0 0.0 9.647309971513852 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTACGG 65 0.0059324047 29.142239 5 TACATGG 8495 0.0 24.528194 2 GTACATG 8880 0.0 24.262774 1 ACATGGG 9160 0.0 21.713514 3 GAGTACT 7785 0.0 21.676914 12-13 CATGGGA 5250 0.0 20.115084 4 AGTACTT 8185 0.0 19.981398 12-13 GTACTTT 8545 0.0 19.55625 14-15 GTATAGG 270 2.325578E-8 19.334211 1 CATGGGG 3945 0.0 18.246218 4 TATTCCG 210 2.0087606E-5 18.040434 5 CCCTATA 475 0.0 17.945484 2 ATGGGAG 2220 0.0 17.91854 5 GTATCAA 21680 0.0 17.86193 1 AGAGTAC 14715 0.0 17.290785 10-11 TACTTTT 9625 0.0 16.968414 14-15 TTTGCGT 450 7.2759576E-12 16.837738 3 GGTATCA 17825 0.0 16.320312 1 TATCAAC 24060 0.0 16.1003 2 ACTTTTT 10555 0.0 15.967849 16-17 >>END_MODULE