FastQCFastQC Report
Fri 27 May 2016
SRR938935_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938935_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2055034
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97890.47634248387131306No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83830.40792512435317374No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT73480.3575609941246714No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53970.26262339211905983No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT36080.17556887136660512No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT29150.141846801561434No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC25020.12174981046542295No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA22710.11050912053036592No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG90950.023.884041
TACATGG89650.023.609062
GAGTACT81750.022.0893112-13
ACATGGG94500.021.1419183
TATTCCG2257.228482E-821.09175
AGTACTT85850.020.59212712-13
CGTAACA1656.3707426E-520.1329864
GTACTTT89150.020.0433114-15
TTTGCGT4550.019.8169273
CATGGGA56100.019.3716564
CATGGGG38550.018.7117024
CCGTTGC3054.620233E-918.671349
CATACCG1300.00760533918.2524325
GTATCAA214950.017.9733521
AGAGTAC152200.017.7731710-11
TACTTTT100450.017.71769114-15
TCCGTTG3007.769813E-817.4006548
ATGGGAT20600.017.2777885
ACTTTTT107050.017.09063516-17
GGTATCA176900.016.5343151
Produced by FastQC (version 0.11.5)