FastQCFastQC Report
Thu 26 May 2016
SRR938934_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938934_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2065028
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99640.48251161727589165No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78860.3818834417741551No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT71770.34754976687967426No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51800.2508440563517783No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT36970.17902904948504328No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT29190.14135401553877236No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC24960.12087003178649393No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA22260.10779514854035877No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATGG87150.025.638852
GTACATG89200.025.513761
ACATGGG91150.022.7998373
GAGTACT79550.021.95807612-13
GTACTTT85000.020.44003314-15
CATGGGA55400.019.9953964
AGTACTT82450.019.69298712-13
CATGGGG38350.019.2567584
GTACCGT1300.00771849818.2057466
AGTACAT66450.017.8102052
GTATCAA221600.017.3305911
AGAGTAC147750.017.0922510-11
ACTTTTT104300.017.08976716-17
ATGGGAG23850.016.8711195
TACTTTT96050.016.6345414-15
CTACACT8650.015.8710554
GGTATCA183200.015.8129841
ATGGGAT20300.015.6239975
GTGTAGC11850.015.6043021
TATCAAC244100.015.5535852
Produced by FastQC (version 0.11.5)