##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938934_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2065028 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.87788543303045 33.0 31.0 34.0 30.0 34.0 2 31.922944870481174 33.0 31.0 34.0 30.0 34.0 3 31.97076746659125 34.0 31.0 34.0 30.0 34.0 4 35.51808014225473 37.0 35.0 37.0 33.0 37.0 5 35.55972751943315 37.0 35.0 37.0 33.0 37.0 6 35.64862800891804 37.0 35.0 37.0 33.0 37.0 7 35.5654867633756 37.0 35.0 37.0 33.0 37.0 8 35.555317893994655 37.0 35.0 37.0 33.0 37.0 9 37.17555548883599 39.0 37.0 39.0 33.0 39.0 10-11 37.07422417516857 39.0 37.0 39.0 33.0 39.0 12-13 36.95251444532471 39.0 37.0 39.0 33.0 39.0 14-15 38.166567717241605 40.0 38.0 41.0 33.0 41.0 16-17 38.1034225686044 40.0 38.0 41.0 33.0 41.0 18-19 38.06375748900257 40.0 38.0 41.0 33.0 41.0 20-21 37.9481072411609 40.0 38.0 41.0 33.0 41.0 22-23 38.01286423234939 40.0 38.0 41.0 33.0 41.0 24-25 37.94392594192428 40.0 38.0 41.0 33.0 41.0 26-27 37.85341482052544 40.0 38.0 41.0 33.0 41.0 28-29 37.72625213798554 40.0 37.5 41.0 32.0 41.0 30-31 37.53452979814317 40.0 37.0 41.0 32.0 41.0 32-33 37.32835462763701 40.0 36.5 41.0 31.5 41.0 34-35 37.00582776601576 40.0 36.0 41.0 30.5 41.0 36-37 36.732606773370634 39.5 36.0 41.0 30.0 41.0 38-39 36.518861003337484 39.0 35.5 41.0 30.0 41.0 40-41 36.35857843089779 39.0 35.0 41.0 30.0 41.0 42-43 36.13738143017916 39.0 35.0 41.0 29.5 41.0 44-45 36.03383537656632 39.0 35.0 41.0 29.0 41.0 46-47 35.77642119138336 39.0 35.0 41.0 27.5 41.0 48-49 35.56393206290665 39.0 35.0 41.0 26.5 41.0 50-51 34.36373526170105 38.0 34.0 40.0 24.5 40.5 52-53 34.4916579823615 38.0 34.0 39.5 25.0 40.5 54-55 34.98339247700273 39.0 35.0 40.0 26.0 41.0 56-57 34.86515219164099 38.5 35.0 40.5 25.0 41.0 58-59 34.65634219003326 38.0 34.0 40.0 25.0 41.0 60-61 34.37469564577333 38.0 34.0 40.0 24.0 41.0 62-63 34.00619483125652 37.0 34.0 40.0 23.0 41.0 64-65 33.58533976294753 37.0 33.5 40.0 22.0 41.0 66-67 33.18621636123093 36.0 33.0 39.0 21.5 41.0 68-69 32.78340051563465 36.0 33.0 39.0 20.0 40.5 70-71 32.34911463670226 35.0 32.0 38.5 19.0 40.0 72-73 31.909984271399708 35.0 32.0 37.5 18.0 40.0 74-75 31.44732347454853 35.0 31.0 37.0 14.0 39.0 76-77 31.027987513970757 35.0 31.0 36.5 9.5 39.0 78-79 30.5967071632927 35.0 31.0 36.0 7.0 38.5 80-81 30.159160069500267 34.0 30.0 35.5 3.5 37.0 82-83 29.783846030174892 34.0 30.0 35.0 2.0 37.0 84-85 29.426611406721847 34.0 30.0 35.0 2.0 36.5 86-87 29.029599840776978 34.0 29.0 35.0 2.0 36.0 88-89 28.60189159662726 34.0 29.0 35.0 2.0 36.0 90-91 28.14906529112438 34.0 29.0 35.0 2.0 35.0 92-93 27.728658158630296 34.0 27.0 35.0 2.0 35.0 94-95 27.237773289272592 34.0 26.5 35.0 2.0 35.0 96-97 26.640488651969854 33.0 25.0 35.0 2.0 35.0 98-99 25.929532916744954 33.0 24.0 35.0 2.0 35.0 100-101 23.346105234408444 30.0 10.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2590305420091745 1101 2 0.279224429615617 1101 3 0.18740833861354744 1101 4 0.08649021639225651 1101 5 0.06113195849999897 1101 6 0.17824378938114904 1101 7 0.26310010860571964 1101 8 0.201069565975466 1101 9 0.09753905597307977 1101 10-11 0.3243575534427592 1101 12-13 0.40130633393243187 1101 14-15 0.1249258425828117 1101 16-17 0.4330408540644015 1101 18-19 0.40877414023789527 1101 20-21 0.45265263064893446 1101 22-23 0.4624446651034333 1101 24-25 0.440633959973745 1101 26-27 0.4600380092148484 1101 28-29 0.3683209387904043 1101 30-31 0.29893369862008967 1101 32-33 0.2649949691647606 1101 34-35 0.10617128770505957 1101 36-37 0.14436944827034637 1101 38-39 0.06619930868846069 1101 40-41 -0.022701875635256386 1101 42-43 -0.12298470085115554 1101 44-45 -0.03905321590652733 1101 46-47 0.05801324883763215 1101 48-49 0.18404318349733018 1101 50-51 0.09899389214395171 1101 52-53 0.17385346025181292 1101 54-55 0.041662026054957835 1101 56-57 0.08562353515726073 1101 58-59 -0.037297817771730024 1101 60-61 -4.543661952496336E-4 1101 62-63 0.08936506559724222 1101 64-65 -0.027819249455106387 1101 66-67 -0.16467290287546632 1101 68-69 -0.1509123162347379 1101 70-71 -0.20216751896221297 1101 72-73 -0.2207059952182071 1101 74-75 -0.3107339391850381 1101 76-77 -0.3970586656560968 1101 78-79 -0.45056467690596236 1101 80-81 -0.47454833152582765 1101 82-83 -0.5170874084384387 1101 84-85 -0.6013576595623853 1101 86-87 -0.6906093839936709 1101 88-89 -0.7324304323016477 1101 90-91 -0.7046485439777506 1101 92-93 -0.744281132067762 1101 94-95 -0.7851005132871691 1101 96-97 -0.7839786479414492 1101 98-99 -0.974784253788652 1101 100-101 -0.39702812048164304 1102 1 0.1600935650296229 1102 2 0.12247301434747015 1102 3 0.13281597263226175 1102 4 0.20541474988505826 1102 5 0.26551485546013254 1102 6 0.28803614350103146 1102 7 0.22680202943856642 1102 8 0.2566037051151753 1102 9 0.40731845041568704 1102 10-11 0.2179137108545106 1102 12-13 0.3519896823396209 1102 14-15 0.4300950789448095 1102 16-17 0.3392421694716461 1102 18-19 0.45886837246762724 1102 20-21 0.5076402393303852 1102 22-23 0.4245807164545141 1102 24-25 0.42574913910559786 1102 26-27 0.39881286941781724 1102 28-29 0.40798414093995206 1102 30-31 0.34004635281142015 1102 32-33 0.4336194531801496 1102 34-35 0.3501104420202168 1102 36-37 0.2884880611675271 1102 38-39 0.2100315456660411 1102 40-41 0.10702270925693824 1102 42-43 0.08382031037049842 1102 44-45 0.11424411588262728 1102 46-47 0.09127033423916231 1102 48-49 0.07290104604017955 1102 50-51 0.08465299332443976 1102 52-53 -0.08685104098457685 1102 54-55 0.05957040552968351 1102 56-57 -0.026896529004126535 1102 58-59 -0.16690214125186742 1102 60-61 -0.16567501293805975 1102 62-63 -0.2200978875727415 1102 64-65 -0.061581679899802566 1102 66-67 -0.08780526785731979 1102 68-69 -0.27983872778247587 1102 70-71 -0.38614455287647687 1102 72-73 -0.4036440583944376 1102 74-75 -0.42749239441346987 1102 76-77 -0.3173486878577805 1102 78-79 -0.44666894849247285 1102 80-81 -0.4777673835693683 1102 82-83 -0.5487084718678226 1102 84-85 -0.6017195420763208 1102 86-87 -0.5072907675819955 1102 88-89 -0.3724163633638504 1102 90-91 -0.39817496551980724 1102 92-93 -0.5026218650473382 1102 94-95 -0.4554210126960214 1102 96-97 -0.622269469689325 1102 98-99 -0.5729726172065561 1102 100-101 -0.5765403808305756 1103 1 0.1118128945059631 1103 2 0.18328810787284056 1103 3 0.24196972645891535 1103 4 0.29151662308973414 1103 5 0.2082905429087205 1103 6 0.25996019037835083 1103 7 0.19501931578669485 1103 8 0.23469624601145256 1103 9 0.38321762911859736 1103 10-11 0.3666452181841535 1103 12-13 0.3560528777112282 1103 14-15 0.3763945219732321 1103 16-17 0.4542903819030286 1103 18-19 0.43292064418345433 1103 20-21 0.44815257227262606 1103 22-23 0.4759885194511 1103 24-25 0.5277772978627198 1103 26-27 0.38700992677375723 1103 28-29 0.460800421232868 1103 30-31 0.49065497575649175 1103 32-33 0.4875360557883468 1103 34-35 0.34018407555178953 1103 36-37 0.3557900530489633 1103 38-39 0.4306922245510165 1103 40-41 0.4158567101470183 1103 42-43 0.39096586471232087 1103 44-45 0.4756409952234293 1103 46-47 0.3823888626474101 1103 48-49 0.2893488350113387 1103 50-51 0.20749705476663394 1103 52-53 0.40090394546200514 1103 54-55 0.3016800196953753 1103 56-57 0.4426363161361948 1103 58-59 0.3782938776824665 1103 60-61 0.4341829795367502 1103 62-63 0.4101907127158526 1103 64-65 0.16311360404780828 1103 66-67 0.14316531918804287 1103 68-69 0.10665922908931691 1103 70-71 0.07721575244644896 1103 72-73 0.08520100101136663 1103 74-75 0.14577350894589358 1103 76-77 0.030046761967572166 1103 78-79 0.04588961443991835 1103 80-81 0.0028572877095065508 1103 82-83 0.06622726736698681 1103 84-85 0.006001189943471985 1103 86-87 0.06676258301341065 1103 88-89 0.21317493007790844 1103 90-91 0.05288193189223023 1103 92-93 -0.20689611623747695 1103 94-95 0.13331481015195124 1103 96-97 -0.05392501041028552 1103 98-99 -0.3122353378711473 1103 100-101 0.023629677563778984 1104 1 0.21269201538508398 1104 2 0.2723923369770702 1104 3 0.15542959991878647 1104 4 0.21623190780502455 1104 5 0.2156498502680293 1104 6 0.2686048790230373 1104 7 0.2305770913444718 1104 8 0.22384709516229861 1104 9 0.24755329345426702 1104 10-11 0.3754031269420608 1104 12-13 0.36059500225335483 1104 14-15 0.3685390441177461 1104 16-17 0.3496383672510248 1104 18-19 0.4085017297645379 1104 20-21 0.2890916332116831 1104 22-23 0.36840610186868616 1104 24-25 0.4805178906033163 1104 26-27 0.4984518182156492 1104 28-29 0.4653625257949727 1104 30-31 0.4297292348307522 1104 32-33 0.30800558625787033 1104 34-35 0.3432376886054129 1104 36-37 0.322410099669014 1104 38-39 0.291301615160414 1104 40-41 0.38541714970745744 1104 42-43 0.38247435622080417 1104 44-45 0.37396933355177 1104 46-47 0.48271853297708134 1104 48-49 0.5365483022108108 1104 50-51 0.5518859991555729 1104 52-53 0.45326158781964665 1104 54-55 0.443691341706689 1104 56-57 0.431350934850812 1104 58-59 0.336915256303854 1104 60-61 0.23845870381247636 1104 62-63 0.15614142866657232 1104 64-65 0.10563774657195779 1104 66-67 0.2884300544527747 1104 68-69 0.18826096069104636 1104 70-71 0.10578052101121216 1104 72-73 0.17884735465772295 1104 74-75 0.19333594650833064 1104 76-77 0.21051296243377493 1104 78-79 -0.0013897728394667297 1104 80-81 -0.004105749253529467 1104 82-83 -0.12462188348216685 1104 84-85 -0.07951329557100806 1104 86-87 0.040009336260165895 1104 88-89 0.1089591458621264 1104 90-91 0.2757590547693525 1104 92-93 0.4030039836626216 1104 94-95 0.21863282546996743 1104 96-97 0.2728515463662724 1104 98-99 0.30904671341090406 1104 100-101 0.7730202869543881 1105 1 0.19528588794361568 1105 2 0.23817315215718082 1105 3 0.2511129507258154 1105 4 0.2543618344155121 1105 5 0.30217517417850814 1105 6 0.37816311819258885 1105 7 0.232856955179102 1105 8 0.23058262112044048 1105 9 0.2925655250389383 1105 10-11 0.24015079388053806 1105 12-13 0.42786509376373516 1105 14-15 0.4144698741828563 1105 16-17 0.39944939250374034 1105 18-19 0.44206662188067014 1105 20-21 0.3868713093659224 1105 22-23 0.32321719183877917 1105 24-25 0.3168489329414257 1105 26-27 0.20731188629832786 1105 28-29 0.37420136657727454 1105 30-31 0.3207692431145759 1105 32-33 0.32184746875699943 1105 34-35 0.17719476803952006 1105 36-37 0.2732945273732241 1105 38-39 0.2577602801703094 1105 40-41 0.2883453522469708 1105 42-43 0.1403394593155909 1105 44-45 0.04791670537900217 1105 46-47 0.25296937753888216 1105 48-49 0.23473362689789923 1105 50-51 0.1959023597090379 1105 52-53 0.17056481462817175 1105 54-55 0.2570184621716223 1105 56-57 0.20692983180779834 1105 58-59 0.21743480742411236 1105 60-61 0.2964830116601078 1105 62-63 0.110388959698561 1105 64-65 0.18520621108741864 1105 66-67 0.21304738058953632 1105 68-69 0.06823235560943886 1105 70-71 -0.05740771595794314 1105 72-73 0.08198407659302731 1105 74-75 0.15023223043641565 1105 76-77 0.13999949474715834 1105 78-79 0.1501822123014236 1105 80-81 -0.02806373473094581 1105 82-83 0.06925145166325208 1105 84-85 0.18756238493223165 1105 86-87 0.05257874564936316 1105 88-89 -0.00415699594659813 1105 90-91 0.016186966080656617 1105 92-93 0.24247472493548372 1105 94-95 0.0039907772943514885 1105 96-97 -0.08540406139463741 1105 98-99 -0.05634437371163159 1105 100-101 0.06177111210097408 1106 1 0.260964613222459 1106 2 0.2780302841557898 1106 3 0.22994891085630442 1106 4 0.18417126209754997 1106 5 0.1881994584054283 1106 6 0.2371973804019376 1106 7 0.1739085006388592 1106 8 0.269883190132866 1106 9 0.3431703327431421 1106 10-11 0.28670057053565046 1106 12-13 0.3469969211615265 1106 14-15 0.3842075193810217 1106 16-17 0.48999251868571037 1106 18-19 0.429756074885816 1106 20-21 0.46837281226274996 1106 22-23 0.4715634497733987 1106 24-25 0.4782718958110479 1106 26-27 0.4866610510371885 1106 28-29 0.4480002549159465 1106 30-31 0.43570581286335397 1106 32-33 0.5943845449679941 1106 34-35 0.437355388480988 1106 36-37 0.410993109122451 1106 38-39 0.5903007301024914 1106 40-41 0.7031217712489948 1106 42-43 0.6838057911690498 1106 44-45 0.6871323253040558 1106 46-47 0.5645413674712572 1106 48-49 0.5352194326478212 1106 50-51 0.42999479714431743 1106 52-53 0.2116634810739626 1106 54-55 0.1276276688446174 1106 56-57 0.2181744748259291 1106 58-59 0.37628417153069904 1106 60-61 0.4591151858996696 1106 62-63 0.4000594132999211 1106 64-65 0.2806870661035106 1106 66-67 0.32057805152822993 1106 68-69 0.3971870904225767 1106 70-71 0.4959430381672263 1106 72-73 0.31273814620734797 1106 74-75 0.3382949644788553 1106 76-77 0.32159799212810825 1106 78-79 0.4446306732810257 1106 80-81 0.4828219453630105 1106 82-83 0.504895952358833 1106 84-85 0.6289178430752358 1106 86-87 0.6588687659750185 1106 88-89 0.852838008370302 1106 90-91 0.589183459279301 1106 92-93 0.5422201301974248 1106 94-95 0.747629759834588 1106 96-97 0.7963872885963603 1106 98-99 0.7156185890923332 1106 100-101 1.1381182205365654 1107 1 0.1008711779946232 1107 2 0.011548455120532708 1107 3 0.12089535778961036 1107 4 0.03866618239119646 1107 5 0.052306779836349904 1107 6 -0.015315925910421413 1107 7 -0.029643616218010038 1107 8 0.10421710489939784 1107 9 0.02194472480772447 1107 10-11 0.1699860545629619 1107 12-13 0.1333084069920787 1107 14-15 0.13448516550690925 1107 16-17 0.15843421275572922 1107 18-19 0.11223752982945001 1107 20-21 0.11633603438013296 1107 22-23 0.0633914962211648 1107 24-25 0.11307225606907423 1107 26-27 0.23388836479143293 1107 28-29 0.24711195779756423 1107 30-31 0.15651923807644863 1107 32-33 0.16217306434940326 1107 34-35 -0.07108882875908762 1107 36-37 0.07782068064921077 1107 38-39 0.17264533473197474 1107 40-41 0.06213574756529283 1107 42-43 0.16431466780795034 1107 44-45 0.18863989505777568 1107 46-47 -0.01296793245622041 1107 48-49 0.06207572837114128 1107 50-51 -0.008227600325113826 1107 52-53 0.060749416446718385 1107 54-55 0.0961775474840394 1107 56-57 0.2504194191091713 1107 58-59 0.23339042670306753 1107 60-61 0.18023085571120845 1107 62-63 -0.029304855007559638 1107 64-65 0.031934403501523434 1107 66-67 0.12609315085004624 1107 68-69 0.07770919178484803 1107 70-71 0.23703985239713177 1107 72-73 0.16455329428771037 1107 74-75 0.23403701758914863 1107 76-77 0.09563143046363365 1107 78-79 0.23301464131178307 1107 80-81 0.10016883691979217 1107 82-83 0.15666341349933433 1107 84-85 0.14784795077757806 1107 86-87 0.3742839224334844 1107 88-89 0.4439445402146056 1107 90-91 0.38107551046555344 1107 92-93 0.4395278395535662 1107 94-95 0.39949293582375134 1107 96-97 0.339232591481494 1107 98-99 0.4247558987403437 1107 100-101 0.5924457981520526 1108 1 0.1524308112027022 1108 2 0.15346850857370953 1108 3 0.04620064460594975 1108 4 0.10306830536400469 1108 5 0.008976446191894638 1108 6 0.10382650092480361 1108 7 0.16042070570551914 1108 8 0.1409435308135656 1108 9 0.2248456322423067 1108 10-11 0.18389044895136664 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-1.848033601298031 2316 84-85 -1.935830553442674 2316 86-87 -1.8581174074624336 2316 88-89 -2.0284422092594347 2316 90-91 -2.2492628663147443 2316 92-93 -2.357988443599236 2316 94-95 -2.4436615813322113 2316 96-97 -2.686150712169667 2316 98-99 -3.1654119022394234 2316 100-101 -4.036226424882994 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6115.0 3 1381.0 4 1331.0 5 1596.0 6 1882.0 7 2361.0 8 2910.0 9 3590.0 10 4480.0 11 6195.0 12 8480.0 13 12804.0 14 12297.0 15 11919.0 16 15080.0 17 19981.0 18 17323.0 19 12354.0 20 12239.0 21 13318.0 22 14483.0 23 16130.0 24 18013.0 25 20636.0 26 24151.0 27 28545.0 28 33704.0 29 40291.0 30 48506.0 31 59339.0 32 73720.0 33 94179.0 34 126756.0 35 182504.0 36 276587.0 37 418285.0 38 363815.0 39 57733.0 40 15.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.39937918723255 18.84004553144953 13.99956100223578 22.761014279082143 2 15.77825717967894 21.014637806262932 39.09740071218809 24.10970430187004 3 19.79403209772303 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22.383151211544806 25.975716502321134 29.375061964394323 22.26607032173974 24-25 22.448357678788362 26.051289461007553 29.557515001104278 21.942837859099807 26-27 22.086135431180217 25.968686003495495 29.915822511835298 22.029356053488986 28-29 21.851369254470544 26.74357069178472 29.571508743519782 21.833551310224955 30-31 22.037600085131903 26.30563661912903 29.36253124876526 22.294232046973804 32-33 21.897049962017405 26.370245691872206 29.39595799275446 22.33674635335592 34-35 22.538017250430677 26.262575198422727 29.183258604799477 22.016148946347116 36-37 22.499386912138846 25.90766835928434 29.557385876160392 22.03555885241642 38-39 22.211018911734605 26.310196860373015 29.470325823165332 22.008458404727044 40-41 22.21800323093598 26.394098734619035 29.35084084902007 22.03705718542491 42-43 21.92380647701186 26.584321742668095 28.8744319035331 22.617439876786946 44-45 22.198630747449027 26.539264585627475 28.810215284450848 22.45188938247265 46-47 22.630337263870125 26.763037744012948 28.35713431751371 22.249490674603223 48-49 22.427251901964386 26.741894458329213 28.300168192151133 22.53068544755527 50-51 22.1542769252349 27.125604664709403 28.50135690364913 22.218761506406572 52-53 22.424787347207555 27.178936171041812 27.95446619570681 22.44181028604382 54-55 22.07077369108899 27.026407726610497 28.078216760713566 22.82460182158695 56-57 22.3775321512489 27.210322286631296 27.765659827273765 22.646485734846042 58-59 22.462175421137946 26.869283834841333 27.94146607928915 22.72707466473157 60-61 22.120614989636387 26.810311205232633 28.602039925032596 22.467033880098384 62-63 22.02454939254704 26.84027311947918 28.603210027983472 22.531967459990305 64-65 21.981486285212533 27.195796965368075 28.22811741509473 22.594599334324663 66-67 22.089430438796715 27.009980292758502 27.941043923519338 22.95954534492545 68-69 21.902585935781445 27.02795338285165 28.20870482017962 22.860755861187286 70-71 22.34460573236166 26.8919149014949 27.851108552308656 22.91237081383478 72-73 22.190888013057418 26.74176646834207 28.08443381278895 22.98291170581156 74-75 21.922721596512513 27.596099910148187 27.52363877184825 22.95753972149105 76-77 22.183393480002074 26.964572682656694 27.666120114914676 23.185913722426555 78-79 22.24286568861921 27.0535941016225 27.83800254132947 22.865537668428814 80-81 22.35598038318236 27.039563763421803 27.679442658008675 22.925013195387166 82-83 22.202894556083088 26.90596990322017 27.96812038906722 22.923015151629517 84-85 22.158616333271482 26.97561790491163 27.749481436674884 23.116284325142004 86-87 22.22574744023315 26.90745880669125 27.745167482079623 23.121626270995982 88-89 22.27026742692602 26.829388274361605 27.483721426348374 23.416622872364 90-91 22.20927845120297 26.69766839195254 27.648836655915193 23.444216500929297 92-93 22.3841904542453 26.82190512216753 27.58866282999579 23.205241593591378 94-95 22.438069934171523 26.675245364896703 27.51009738600586 23.376587314925914 96-97 22.151558513520918 26.543917869777804 27.74133364184939 23.56318997485188 98-99 22.346652026083166 26.734759345002768 27.27803281611445 23.640555812799615 100-101 22.630437847827565 26.042444673302356 27.79917401721857 23.527943461651514 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3474.0 1 2135.5 2 897.5 3 1021.5 4 905.5 5 907.0 6 1441.5 7 2552.5 8 3896.5 9 4486.5 10 4010.0 11 3526.5 12 3607.5 13 3776.0 14 4003.5 15 4278.5 16 4558.0 17 4817.5 18 4866.0 19 5060.0 20 5470.0 21 6012.0 22 6798.0 23 7921.0 24 9105.5 25 10654.5 26 13152.0 27 16249.0 28 18493.0 29 20486.0 30 23270.0 31 26896.0 32 30390.5 33 32264.0 34 34133.0 35 36986.5 36 40370.5 37 43492.0 38 46395.0 39 48799.0 40 51230.5 41 54881.5 42 57348.5 43 59737.5 44 63343.0 45 66564.5 46 70417.5 47 78195.5 48 93725.5 49 95591.0 50 83227.0 51 77445.0 52 75395.0 53 74760.5 54 72717.0 55 68108.0 56 62854.5 57 56915.0 58 51797.0 59 46537.5 60 39099.0 61 32249.5 62 26069.5 63 19328.5 64 13475.5 65 9613.0 66 6893.5 67 5115.5 68 3786.0 69 2784.0 70 2047.0 71 1280.5 72 860.5 73 568.0 74 366.0 75 278.5 76 180.5 77 96.0 78 65.0 79 52.5 80 38.0 81 29.0 82 28.0 83 19.5 84 14.5 85 15.5 86 13.0 87 8.0 88 7.5 89 7.5 90 6.0 91 5.0 92 4.0 93 3.0 94 2.0 95 0.5 96 0.5 97 1.0 98 1.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.28048045837635127 2 0.08760171774910558 3 0.09157260821645033 4 0.10527702287814016 5 0.10726246811181253 6 0.08275916839868516 7 0.09249269259303021 8 0.08910290804773592 9 0.09423601035918157 10-11 0.08704482457380723 12-13 0.09149996997619403 14-15 0.10065238824848864 16-17 0.08876392959320649 18-19 0.09975651661866088 20-21 0.10181460009258954 22-23 0.11387254797513642 24-25 0.1257367938836665 26-27 0.12878759997443134 28-29 0.12108794650726286 30-31 0.11363042050761539 32-33 0.10852153094292184 34-35 0.37791255130681034 36-37 0.5760938834727665 38-39 0.5601618961098832 40-41 0.5241817544362595 42-43 0.5496051385259667 44-45 0.5709849939080729 46-47 0.5605977255514211 48-49 0.5820260064270315 50-51 0.5725830351937117 52-53 0.5770381805960985 54-55 0.5669898906939761 56-57 0.5395568486238443 58-59 0.5345932355396633 60-61 0.48432757328229936 62-63 0.5566268350840764 64-65 0.5920258708356497 66-67 0.6044954354129822 68-69 0.6063598169128942 70-71 0.6013719910819612 72-73 0.5931396571862464 74-75 0.5909605099785572 76-77 0.5739631617585815 78-79 0.5510094778375887 80-81 0.5462395667274246 82-83 0.5594355137073203 84-85 0.5694353781159384 86-87 0.5602587470968917 88-89 0.5747621824014009 90-91 0.5874738744462544 92-93 0.5953188043939356 94-95 0.5941323798030825 96-97 0.5903309785630025 98-99 0.5949798259394062 100-101 0.5732367793560184 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2065028.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.9558010236154 #Duplication Level Percentage of deduplicated Percentage of total 1 70.3657872688359 33.04081905865508 2 14.42645224148561 13.548112418557775 3 5.850427391753303 8.24134513530832 4 2.902150013168116 5.450911142360195 5 1.7124676926743947 4.020514611829432 6 1.1037085970702136 3.1095312763249594 7 0.7094372392994612 2.331853569310197 8 0.5121432995545173 1.9238479095567818 9 0.3986413993037518 1.6846673602934328 >10 1.8686787895968102 15.343917284933054 >50 0.09764004423827696 3.141263971134482 >100 0.04720036408632722 4.079659433015989 >500 0.002890950002583843 0.9255997503731177 >1k 0.001961716073181891 1.6934907228306804 >5k 4.1299285751197716E-4 1.4644663555164856 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9964 0.48251161727589165 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7886 0.3818834417741551 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7177 0.34754976687967426 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5180 0.2508440563517783 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3697 0.17902904948504328 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2919 0.14135401553877236 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2496 0.12087003178649393 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2226 0.10779514854035877 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 4.8425493504204304E-5 0.0 12-13 0.0 0.0 0.0 4.8425493504204304E-5 0.0 14-15 0.0 0.0 0.0 4.8425493504204304E-5 0.0 16-17 0.0 0.0 0.0 1.2106373376051077E-4 0.0 18-19 0.0 0.0 0.0 1.4527648051261292E-4 0.0 20-21 0.0 0.0 0.0 2.6634021427312366E-4 0.0 22-23 0.0 0.0 0.0 3.8740394803363443E-4 0.0 24-25 1.4527648051261292E-4 0.0 0.0 7.99020642819371E-4 0.0 26-27 1.4527648051261292E-4 0.0 0.0 0.0020822962206807853 0.0 28-29 1.4527648051261292E-4 0.0 0.0 0.0074333132528953605 0.0 30-31 1.4527648051261292E-4 0.0 0.0 0.021791472076891936 0.0 32-33 1.4527648051261292E-4 0.0 0.0 0.050653066205397695 0.0 34-35 1.4527648051261292E-4 0.0 0.0 0.09665728503439178 0.0 36-37 1.9370197401681721E-4 0.0 0.0 0.17089356657633697 0.0 38-39 3.3897845452943013E-4 0.0 0.0 0.27537156881165775 0.0 40-41 3.3897845452943013E-4 0.0 0.0 0.3863385871765419 0.0 42-43 4.116166947857366E-4 0.0 0.0 0.5047631315410734 0.0 44-45 4.358294415378387E-4 0.0 0.0 0.647327784417451 0.0 46-47 4.358294415378387E-4 0.0 0.0 0.8210300296170319 0.0 48-49 4.358294415378387E-4 0.0 0.0 1.0037878421018989 0.0 50-51 4.358294415378387E-4 0.0 0.0 1.2031071733652037 0.0 52-53 4.358294415378387E-4 0.0 0.0 1.3959859139924495 0.0 54-55 4.358294415378387E-4 0.0 0.0 1.6118909767809444 0.0 56-57 4.84254935042043E-4 0.0 0.0 1.8744782153074921 0.0 58-59 4.84254935042043E-4 0.0 0.0 2.1511330596970115 0.0 60-61 4.84254935042043E-4 0.0 0.0 2.4570126894163176 0.0 62-63 4.84254935042043E-4 0.0 0.0 2.7699624411872383 0.0 64-65 4.84254935042043E-4 0.0 0.0 3.0985536273600163 0.0 66-67 5.326804285462473E-4 0.0 0.0 3.474964988368196 0.0 68-69 5.811059220504517E-4 0.0 0.0 3.9108428553995394 0.0 70-71 6.295314155546559E-4 0.0 0.0 4.368826960215552 0.0 72-73 6.295314155546559E-4 0.0 0.0 4.828820723011988 0.0 74-75 6.53744162306758E-4 0.0 0.0 5.309637448015232 0.0 76-77 6.779569090588603E-4 0.0 0.0 5.8313010767892735 0.0 78-79 6.779569090588603E-4 0.0 0.0 6.4240775427742385 0.0 80-81 7.748078960672689E-4 0.0 0.0 7.067507074964601 0.0 82-83 7.748078960672689E-4 0.0 0.0 7.729241443699553 0.0 84-85 7.748078960672689E-4 0.0 0.0 8.401823122979447 0.0 86-87 7.748078960672689E-4 0.0 0.0 9.117890895426115 0.0 88-89 7.748078960672689E-4 0.0 0.0 9.866936428949147 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACATGG 8715 0.0 25.63885 2 GTACATG 8920 0.0 25.51376 1 ACATGGG 9115 0.0 22.799837 3 GAGTACT 7955 0.0 21.958076 12-13 GTACTTT 8500 0.0 20.440033 14-15 CATGGGA 5540 0.0 19.995396 4 AGTACTT 8245 0.0 19.692987 12-13 CATGGGG 3835 0.0 19.256758 4 GTACCGT 130 0.007718498 18.205746 6 AGTACAT 6645 0.0 17.810205 2 GTATCAA 22160 0.0 17.330591 1 AGAGTAC 14775 0.0 17.09225 10-11 ACTTTTT 10430 0.0 17.089767 16-17 ATGGGAG 2385 0.0 16.871119 5 TACTTTT 9605 0.0 16.63454 14-15 CTACACT 865 0.0 15.871055 4 GGTATCA 18320 0.0 15.812984 1 ATGGGAT 2030 0.0 15.623997 5 GTGTAGC 1185 0.0 15.604302 1 TATCAAC 24410 0.0 15.553585 2 >>END_MODULE