FastQCFastQC Report
Thu 26 May 2016
SRR938934_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938934_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2065028
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100470.4865309332367406No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84980.41151984379872814No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT75270.3644986896061458No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54860.2656622573640648No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT36950.1789321984980349No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT28430.1376736780324528No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC24280.11757709822820804No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA21380.1035337051119888No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATGG89850.023.1852342
GTACATG91350.023.0457381
GAGTACT87000.021.874212-13
ACATGGG95450.020.8306643
GTACTTT92750.020.21111514-15
AGTACTT91650.019.57340612-13
CATGGGG38200.019.1303254
GTATCAA211850.018.8405021
GGTATCA171650.018.2582441
AGAGTAC160350.017.77260810-11
TACTTTT100500.017.75533514-15
GTACGGA1350.00925648617.6405931
ACTTTTT109500.017.1836416-17
CATGGGA57550.017.068294
GTGTAGC10800.016.7585621
GTATTAC6550.016.7248951
CAACGCA239650.016.5140515
TATCAAC240200.016.4959952
TCAACGC241400.016.3943354
ATCAACG241550.016.3841553
Produced by FastQC (version 0.11.5)