##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938934_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2065028 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.12073395614975 33.0 31.0 34.0 30.0 34.0 2 32.343619553826876 34.0 31.0 34.0 30.0 34.0 3 32.434201860701165 34.0 31.0 34.0 30.0 34.0 4 36.02193868557715 37.0 35.0 37.0 35.0 37.0 5 35.93786428077489 37.0 35.0 37.0 35.0 37.0 6 35.98138524029699 37.0 35.0 37.0 35.0 37.0 7 35.920966204816594 37.0 35.0 37.0 35.0 37.0 8 35.892669736197284 37.0 35.0 37.0 35.0 37.0 9 37.56113815405893 39.0 37.0 39.0 35.0 39.0 10-11 37.483670197207985 39.0 37.0 39.0 34.5 39.0 12-13 37.4094900892385 39.0 37.0 39.0 34.0 39.0 14-15 38.64791300650645 40.0 38.0 41.0 34.0 41.0 16-17 38.63965355433437 40.0 38.0 41.0 34.0 41.0 18-19 38.749905812415136 40.0 38.0 41.0 34.0 41.0 20-21 38.74570393234377 40.0 38.0 41.0 34.0 41.0 22-23 38.67966536046969 40.0 38.0 41.0 34.0 41.0 24-25 38.611663134834004 40.0 38.0 41.0 34.0 41.0 26-27 38.44273879095101 40.0 38.0 41.0 34.0 41.0 28-29 38.25133363809111 40.0 38.0 41.0 33.5 41.0 30-31 38.06337008505454 40.0 37.5 41.0 33.0 41.0 32-33 37.87878759997443 40.0 37.0 41.0 33.0 41.0 34-35 37.83517245286747 40.0 37.0 41.0 33.0 41.0 36-37 37.71524550756697 40.0 37.0 41.0 33.0 41.0 38-39 37.56927484760497 40.0 37.0 41.0 33.0 41.0 40-41 37.39966988341078 40.0 37.0 41.0 32.0 41.0 42-43 37.20565290155872 40.0 36.0 41.0 31.5 41.0 44-45 36.92951499931236 40.0 36.0 41.0 31.0 41.0 46-47 36.57662680602878 39.5 36.0 41.0 30.0 41.0 48-49 36.24444293249293 39.0 35.0 41.0 30.0 41.0 50-51 35.952633329911265 39.0 35.0 41.0 29.0 41.0 52-53 35.71201455864037 39.0 35.0 40.0 28.0 41.0 54-55 35.44749925908995 38.5 35.0 40.0 28.0 41.0 56-57 35.15200036028567 38.0 34.5 40.0 27.0 41.0 58-59 34.83457681929737 38.0 34.0 40.0 26.5 41.0 60-61 34.4071387894014 37.5 34.0 40.0 26.0 41.0 62-63 34.077528730845295 37.0 33.5 40.0 26.0 41.0 64-65 33.79792913219579 36.5 33.0 39.0 25.5 41.0 66-67 33.33212600507112 36.0 33.0 39.0 23.5 40.5 68-69 32.904307108668746 35.5 32.5 39.0 23.0 40.0 70-71 32.4280423800549 35.0 32.0 38.0 22.0 40.0 72-73 31.907441690863273 35.0 31.0 37.0 20.5 39.0 74-75 31.444057901394075 35.0 31.0 36.5 19.0 39.0 76-77 29.47759981946976 32.5 28.5 35.0 15.0 37.0 78-79 30.45726740751215 34.0 30.0 35.0 17.0 37.0 80-81 30.464373122301488 34.0 31.0 35.0 15.5 37.0 82-83 30.15060691671009 34.0 30.0 35.0 11.0 37.0 84-85 29.764270508680752 34.0 30.0 35.0 7.0 36.0 86-87 29.195136095006944 34.0 29.0 35.0 2.0 36.0 88-89 28.62422567635887 34.0 29.0 35.0 2.0 35.0 90-91 28.154096457772003 33.0 28.0 35.0 2.0 35.0 92-93 27.794764526195287 33.0 27.0 35.0 2.0 35.0 94-95 27.393985456855788 33.0 27.0 35.0 2.0 35.0 96-97 26.766457403967404 33.0 25.5 35.0 2.0 35.0 98-99 25.851793777130382 32.5 24.5 34.0 2.0 35.0 100-101 21.550408759590667 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.10787390583733014 1101 2 0.13245259335744208 1101 3 0.0962664083500897 1101 4 0.10723640502534693 1101 5 0.12639152422689648 1101 6 0.07439042904536564 1101 7 0.07665797565686461 1101 8 0.13115151534906744 1101 9 0.1421631223102935 1101 10-11 0.2220639799439894 1101 12-13 0.19584576841557322 1101 14-15 0.1845851676862864 1101 16-17 0.15569459999468904 1101 18-19 0.22450864000904147 1101 20-21 0.16424947842761384 1101 22-23 0.21875683793680167 1101 24-25 0.24965524576808207 1101 26-27 0.21456588333563786 1101 28-29 0.1681176617001512 1101 30-31 0.18579365308359996 1101 32-33 0.22391016991414858 1101 34-35 0.29193869633557057 1101 36-37 0.26843307652065107 1101 38-39 0.3945237032174802 1101 40-41 0.38202756992911446 1101 42-43 0.2958238592091078 1101 44-45 0.23425131043727276 1101 46-47 0.24669944476351446 1101 48-49 0.020761346474429843 1101 50-51 0.10275303815134862 1101 52-53 0.07244618962755567 1101 54-55 0.11417020307533932 1101 56-57 -0.12591890124253524 1101 58-59 -0.021492583588610614 1101 60-61 0.012861324096967053 1101 62-63 -0.07963484987968883 1101 64-65 -0.11596656459740728 1101 66-67 -0.0012081994309411925 1101 68-69 -0.07573372794481514 1101 70-71 -0.09185406387970829 1101 72-73 -0.10630396378940787 1101 74-75 -0.010257883553283165 1101 76-77 -0.11035210867180112 1101 78-79 0.004390309359042277 1101 80-81 0.08371462828503695 1101 82-83 0.10445415289745696 1101 84-85 0.031170358097845963 1101 86-87 0.3060853003284869 1101 88-89 0.4447414264056313 1101 90-91 0.42406276758122274 1101 92-93 0.31753812505892753 1101 94-95 0.18939848242974477 1101 96-97 0.16965160983233574 1101 98-99 0.20970330372404078 1101 100-101 0.7932619876306255 1102 1 0.09872278117347122 1102 2 0.15785054056652825 1102 3 0.17730694962529014 1102 4 0.20293593380928598 1102 5 0.1228828014305492 1102 6 0.09600563316302413 1102 7 0.16739493649172488 1102 8 0.1160274072192351 1102 9 0.1108251399673037 1102 10-11 0.26242994255971297 1102 12-13 0.17743424045315237 1102 14-15 0.25285040505431056 1102 16-17 0.2398482095119263 1102 18-19 0.23322937708519476 1102 20-21 0.2682928977396202 1102 22-23 0.18236320860663824 1102 24-25 0.16784961676520993 1102 26-27 0.20806792311196887 1102 28-29 0.3619510518693545 1102 30-31 0.267461448230641 1102 32-33 0.32101343492911383 1102 34-35 0.41798336438935735 1102 36-37 0.3421563423813012 1102 38-39 0.3537031909038646 1102 40-41 0.3116390871284196 1102 42-43 0.299708604845236 1102 44-45 0.376723742293251 1102 46-47 0.5018325203310638 1102 48-49 0.4180613954027024 1102 50-51 0.6748279264153183 1102 52-53 0.5879006047864266 1102 54-55 0.7097059511919284 1102 56-57 0.574223133525642 1102 58-59 0.4988081193640497 1102 60-61 0.46784333156195856 1102 62-63 0.4887185242766279 1102 64-65 0.43210093335567734 1102 66-67 0.5950992260797463 1102 68-69 0.6399681566448621 1102 70-71 0.3902847825882034 1102 72-73 0.5471596197919943 1102 74-75 0.6138620527685745 1102 76-77 0.3235005452888906 1102 78-79 0.14891844256695563 1102 80-81 0.4680276802725629 1102 82-83 0.5241765027944645 1102 84-85 0.4780650601707883 1102 86-87 0.5357067569413019 1102 88-89 0.6825074361374703 1102 90-91 0.6460411721461021 1102 92-93 0.6658916419913474 1102 94-95 0.7675843253002483 1102 96-97 0.6258936164296891 1102 98-99 0.6830133700435432 1102 100-101 0.9468178265418388 1103 1 0.10663825095809898 1103 2 0.06461080003706599 1103 3 0.049318116028977954 1103 4 0.05656164350975956 1103 5 0.07305178094532039 1103 6 0.0944935834266758 1103 7 0.08900408539059868 1103 8 0.07863371057320023 1103 9 0.10754885239195033 1103 10-11 0.1713971850783551 1103 12-13 0.14814023228063888 1103 14-15 0.15021456737174788 1103 16-17 0.0735604992989849 1103 18-19 0.0771256158412541 1103 20-21 0.17480501490594946 1103 22-23 0.20385961458902813 1103 24-25 0.13813821705380747 1103 26-27 0.12374566178036162 1103 28-29 0.2552714386476893 1103 30-31 0.28453107642176434 1103 32-33 0.20554594291956363 1103 34-35 0.0672174947449875 1103 36-37 0.042202194763603984 1103 38-39 0.2149824723700604 1103 40-41 0.23696160469392424 1103 42-43 0.19297248530537559 1103 44-45 0.11768232680324076 1103 46-47 0.13487954599490592 1103 48-49 0.030628454511813175 1103 50-51 -0.11989999363970583 1103 52-53 0.08132213652571352 1103 54-55 0.16357373449896784 1103 56-57 -0.05212890488247268 1103 58-59 -0.04956870630248744 1103 60-61 -0.11688202615405174 1103 62-63 0.03266277317414534 1103 64-65 0.08911917990663198 1103 66-67 0.09871158464537189 1103 68-69 0.01714971859278336 1103 70-71 0.010207238118134399 1103 72-73 0.0339024823292533 1103 74-75 0.012092690251549243 1103 76-77 0.05952406486381179 1103 78-79 0.09185272531432176 1103 80-81 0.13970506830509422 1103 82-83 0.14195468515208276 1103 84-85 0.16756297658459474 1103 86-87 0.1683195335727703 1103 88-89 0.25360034115560737 1103 90-91 0.5581782977411756 1103 92-93 0.38953191656620234 1103 94-95 0.30042515010368476 1103 96-97 0.199893562485709 1103 98-99 0.3815138622823504 1103 100-101 0.843739917949911 1104 1 -0.00778732346747546 1104 2 -0.013597658171391913 1104 3 -0.05957299286212958 1104 4 0.0026755296236515846 1104 5 0.015289543183087062 1104 6 5.475294806274178E-4 1104 7 -0.004575668189154669 1104 8 0.08761139955088737 1104 9 0.07306333790643293 1104 10-11 0.016129119810294412 1104 12-13 0.08763074177115016 1104 14-15 0.1847866619438392 1104 16-17 0.04394677968525684 1104 18-19 0.050918489634121045 1104 20-21 0.12964683974777103 1104 22-23 0.018448359177774876 1104 24-25 0.10716585608145124 1104 26-27 -0.03696362892893035 1104 28-29 0.04097573435198143 1104 30-31 -0.009008717118028642 1104 32-33 0.030294527668146998 1104 34-35 0.03535935288683589 1104 36-37 0.09414691670832553 1104 38-39 0.2549325223201109 1104 40-41 0.1624594401917605 1104 42-43 0.09135410368699581 1104 44-45 0.12144856056947617 1104 46-47 0.09117991229527433 1104 48-49 0.12954953343289333 1104 50-51 0.04171505797534536 1104 52-53 0.05145200665558036 1104 54-55 0.10832564925087951 1104 56-57 0.057794505040938304 1104 58-59 0.19009496336118303 1104 60-61 0.28210565283362854 1104 62-63 0.21450093501230327 1104 64-65 0.1558224766099272 1104 66-67 0.24259103852482866 1104 68-69 0.1769998684429268 1104 70-71 0.19725019516108944 1104 72-73 0.09791846634523793 1104 74-75 -0.015243307084450208 1104 76-77 -0.04844130310155492 1104 78-79 0.036321589783185004 1104 80-81 0.12854289714292122 1104 82-83 0.23450879770619792 1104 84-85 0.10252468154630279 1104 86-87 0.05790661315984735 1104 88-89 0.17892168647695428 1104 90-91 0.3238692734321482 1104 92-93 0.3937477007819865 1104 94-95 0.21874016841870514 1104 96-97 0.227792330384478 1104 98-99 0.2808478616163512 1104 100-101 0.9610226352326237 1105 1 0.005310179736035536 1105 2 0.06585535053957159 1105 3 0.036376993547222014 1105 4 0.06885112081690892 1105 5 0.13290768475881976 1105 6 0.10917487386768698 1105 7 0.07533605730560566 1105 8 0.12413322872289712 1105 9 0.01838543630273648 1105 10-11 0.08377936120611906 1105 12-13 -0.016658078948765365 1105 14-15 0.05323875166801173 1105 16-17 0.0473753770007832 1105 18-19 0.10424690444663298 1105 20-21 0.15018567664703397 1105 22-23 0.1119785627786527 1105 24-25 0.13034050240096917 1105 26-27 0.061989891049321955 1105 28-29 0.04756642281545709 1105 30-31 0.15199103695592697 1105 32-33 0.08356806214310097 1105 34-35 0.28185840154136343 1105 36-37 0.23798944638123487 1105 38-39 0.11395360724225867 1105 40-41 0.30405627680627134 1105 42-43 0.21575436773390777 1105 44-45 0.33584028192232296 1105 46-47 0.3168987282261071 1105 48-49 0.2398496926376481 1105 50-51 0.1921517708502165 1105 52-53 0.3057175291189864 1105 54-55 0.29186064193781647 1105 56-57 0.24308838665990606 1105 58-59 0.11561600298116304 1105 60-61 0.08460115497577902 1105 62-63 -0.0012347074371135136 1105 64-65 0.03946748884151674 1105 66-67 0.22687183428971736 1105 68-69 0.17954520296706278 1105 70-71 0.21547913936176855 1105 72-73 0.07648795339529002 1105 74-75 0.048153449280505356 1105 76-77 0.08069886179832864 1105 78-79 0.3295508575707551 1105 80-81 0.30278615682787446 1105 82-83 0.368313273560144 1105 84-85 0.13425289444059274 1105 86-87 0.26746964141545604 1105 88-89 0.29004841604891496 1105 90-91 0.3633464864429321 1105 92-93 0.4493777244685475 1105 94-95 0.18958214152216257 1105 96-97 -0.02768754898232828 1105 98-99 0.06426545697740949 1105 100-101 0.6693688473244208 1106 1 0.0410332406607381 1106 2 0.015036402448664887 1106 3 -0.004468517547550732 1106 4 -0.01504948673068185 1106 5 0.04309498436521153 1106 6 0.032714382137719156 1106 7 0.1012990640487601 1106 8 0.07187994407956211 1106 9 -0.002531382530705173 1106 10-11 0.11779674849642419 1106 12-13 0.1311813632357257 1106 14-15 0.09172038521573 1106 16-17 0.061549298893581295 1106 18-19 -0.0621778021014876 1106 20-21 0.005854045914958306 1106 22-23 0.10418289311140683 1106 24-25 0.053139483921015085 1106 26-27 0.07719537364928186 1106 28-29 0.0743835921270275 1106 30-31 0.19482037691616227 1106 32-33 0.11069293199852126 1106 34-35 0.036479528359194546 1106 36-37 0.022774948673017548 1106 38-39 0.2300058282038222 1106 40-41 0.3466832187477067 1106 42-43 0.43532301121768313 1106 44-45 0.22529548403120714 1106 46-47 0.32327660166559724 1106 48-49 0.10666270540349387 1106 50-51 0.07434816940285316 1106 52-53 0.26695976053252224 1106 54-55 0.14128661691420064 1106 56-57 0.22635930025905537 1106 58-59 0.2307508041020654 1106 60-61 0.1777137132741089 1106 62-63 0.25471873622060315 1106 64-65 0.2647727209628741 1106 66-67 0.33815741145487976 1106 68-69 0.3626099811916177 1106 70-71 0.38748184944977737 1106 72-73 0.2857585402283327 1106 74-75 0.362306108649058 1106 76-77 -0.019773635934637213 1106 78-79 0.280063973795702 1106 80-81 0.38725481720551613 1106 82-83 0.6075786659736657 1106 84-85 0.48572153638397353 1106 86-87 0.3536605157521677 1106 88-89 0.34067127922204676 1106 90-91 0.5186927107918571 1106 92-93 0.5448063070081766 1106 94-95 0.6860651129422486 1106 96-97 0.623971445583102 1106 98-99 0.6416018283433012 1106 100-101 1.1331725563470272 1107 1 -0.0397250060380685 1107 2 0.042598382075283325 1107 3 -0.12189042226816582 1107 4 -0.13268961156427395 1107 5 -0.13497335847222303 1107 6 -0.046852665261340576 1107 7 -0.08601026734527295 1107 8 -0.06468980135790758 1107 9 -0.004911345637864883 1107 10-11 0.026778259699931084 1107 12-13 0.007786535344664003 1107 14-15 0.0763510890778889 1107 16-17 0.08906524771511926 1107 18-19 0.031168408491637933 1107 20-21 -0.05380333876569665 1107 22-23 -0.006154042639821 1107 24-25 -0.05031548114672546 1107 26-27 -0.025376481898746306 1107 28-29 0.01158917154770478 1107 30-31 0.0023901793441538643 1107 32-33 0.06088199926827542 1107 34-35 -0.023491669508480584 1107 36-37 0.06742507315102841 1107 38-39 0.10651966935029833 1107 40-41 0.022949540808447466 1107 42-43 0.04911457106771877 1107 44-45 -0.016993503695374557 1107 46-47 -1.1259402221241999E-5 1107 48-49 0.018098705779536317 1107 50-51 0.13902437313276295 1107 52-53 0.16065356459131408 1107 54-55 0.14396992059135272 1107 56-57 0.32940437196726435 1107 58-59 0.2171868166555626 1107 60-61 -0.0030290436934734544 1107 62-63 0.03235877337647963 1107 64-65 0.0658419508066217 1107 66-67 0.15737519951151313 1107 68-69 0.10221895315568474 1107 70-71 0.08288797510267187 1107 72-73 0.2348707545633495 1107 74-75 0.16861907525894537 1107 76-77 -0.1824367591223286 1107 78-79 0.13309211883219518 1107 80-81 0.40604695426723225 1107 82-83 0.41047439329204494 1107 84-85 0.3873705108225103 1107 86-87 0.34764046580509245 1107 88-89 0.5793955585963957 1107 90-91 0.8086079946265663 1107 92-93 0.8019852859815977 1107 94-95 0.6385778834462954 1107 96-97 0.5167959006538716 1107 98-99 0.5758949242583498 1107 100-101 1.0504676206269785 1108 1 -0.09611087460052659 1108 2 -0.07116182551827421 1108 3 -0.028827083894896077 1108 4 0.00477371441006369 1108 5 -0.017587201093355986 1108 6 -0.06100958949943447 1108 7 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-1.0358829216316252 2316 62-63 -1.1635460956772121 2316 64-65 -1.0358754109946915 2316 66-67 -1.0625570948616527 2316 68-69 -1.2500029215132287 2316 70-71 -1.251394683276537 2316 72-73 -1.258677787142208 2316 74-75 -1.4145511322471975 2316 76-77 -1.5116112247170719 2316 78-79 -1.5212976476287956 2316 80-81 -1.4686963748113762 2316 82-83 -1.8323438660376574 2316 84-85 -1.900880393516804 2316 86-87 -1.9836252056258559 2316 88-89 -2.058939347135375 2316 90-91 -2.337096582818681 2316 92-93 -2.6704531760613754 2316 94-95 -2.883247343206115 2316 96-97 -3.201244470465795 2316 98-99 -3.6544278126230516 2316 100-101 -4.184605104902886 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 4.0 4 10.0 5 55.0 6 117.0 7 332.0 8 725.0 9 1385.0 10 2178.0 11 3580.0 12 4944.0 13 6995.0 14 8230.0 15 11206.0 16 14110.0 17 19853.0 18 19317.0 19 13488.0 20 12182.0 21 12977.0 22 14079.0 23 15174.0 24 17021.0 25 20522.0 26 24117.0 27 29265.0 28 35698.0 29 43341.0 30 52291.0 31 63714.0 32 78531.0 33 99432.0 34 133734.0 35 192999.0 36 301992.0 37 461581.0 38 327298.0 39 22548.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.195346537397334 19.540001876709884 14.573381544357206 22.691270041535578 2 15.990533784529799 21.540482744059645 38.41347429671656 24.055509174694 3 19.69489033562741 24.972736447157132 30.55091746939993 24.781455747815524 4 13.267616710281892 17.066693526673728 36.54056990994795 33.12511985309642 5 13.436266811556093 37.83517181164568 34.08862758429325 14.639933792504975 6 31.35357002423212 36.16716092953703 17.15100231086455 15.3282667353663 7 27.45110477920881 33.55412130005017 20.930127823932654 18.064646096808374 8 26.093011813883393 34.10946485955638 20.04791218327306 19.74961114328716 9 26.09930712803894 16.73149226063763 20.662480121334916 36.50672048998852 10-11 25.02755410580389 26.459617012456977 27.128034099295505 21.384794782443628 12-13 26.227440983851068 24.1113679814511 27.723280265449183 21.93791076924865 14-15 22.965990130283075 25.358044187334304 26.608284199034042 25.067681483348576 16-17 21.564743358786316 28.46501474103431 27.480261348271952 22.489980551907422 18-19 21.609658561530402 27.162464625176995 29.701752228056954 21.52612458523565 20-21 22.756228971229444 25.982553263200302 29.468317136619937 21.79290062895031 22-23 22.26858425164211 26.09063896470169 29.46078697238004 22.179989811276165 24-25 22.410979415291223 26.114585371239517 29.645506017351824 21.82892919611744 26-27 22.07347793831367 26.014247748698804 30.022159505827524 21.89011480716 28-29 21.79946228332013 26.811331371778007 29.651534991293094 21.737671353608764 30-31 21.996118212440702 26.31712499782085 29.424274150277867 22.26248263946058 32-33 21.872560565764726 26.337899534534152 29.499527367183397 22.290012532517718 34-35 22.44917873376136 26.275846472269492 29.368197882596554 21.906776911372596 36-37 22.469934044497567 25.9528291515669 29.64740756955499 21.929829234380545 38-39 22.138322379294287 26.348538222416273 29.574549337006395 21.938590061283048 40-41 22.11802945893802 26.42638461657666 29.49997966078056 21.95560626370476 42-43 21.90026314591572 26.589480749283055 28.967067535619613 22.543188569181613 44-45 22.133977265133254 26.599522910651803 28.89988581235454 22.366614011860406 46-47 22.626894018727658 26.80496392166567 28.37378611979881 22.19435593980786 48-49 22.384617861777027 26.763198489505058 28.355702382327326 22.496481266390592 50-51 22.073156853582176 27.148834269968987 28.528461052341026 22.249547824107815 52-53 22.463157884542 27.19566030097413 27.952478126204582 22.38870368827929 54-55 22.037860987841327 27.07476605644088 27.961436842502863 22.925936113214927 56-57 22.28219181531679 27.31420590907242 27.740761868604203 22.662840407006588 58-59 22.427463312775057 26.952907084857124 27.848344619723548 22.771284982644268 60-61 22.089509233129096 26.959647357786327 28.453001417687556 22.49784199139702 62-63 21.940598384138134 26.91428397096795 28.45610325864831 22.68901438624561 64-65 21.8732029571562 27.327932428888857 28.063880113771045 22.7349845001839 66-67 22.017074654822867 27.060826672083966 27.827101383759395 23.094997289333776 68-69 21.823204085452417 27.07854365079077 28.045717512302144 23.05253475145467 70-71 22.182464882428928 26.98114189762606 27.67173802770181 23.164655192243202 72-73 22.104567105143367 26.73707087748931 27.858338966832413 23.300023050534907 74-75 21.774063111977174 27.585945565871263 27.3110824647414 23.328908857410166 76-77 22.00018036372381 26.90760092489742 27.58738307267058 23.504835638708187 78-79 22.00542656342992 26.91156096264117 27.69142970043491 23.391582773494 80-81 22.08754554417664 26.936341783259117 27.558173545346598 23.417939127217647 82-83 21.929339456898404 26.769540170883882 27.826039162665104 23.475081209552606 84-85 21.796193562508595 26.841355177750614 27.596308621481164 23.766142638259627 86-87 21.790186233529795 26.76532906947251 27.578527639197453 23.86595705780024 88-89 21.859918889709267 26.60649543815413 27.343813078620947 24.189772593515652 90-91 21.666806358785237 26.482443130123816 27.4783143169845 24.37243619410645 92-93 21.72735672349237 26.62080126758572 27.42863535022285 24.22320665869906 94-95 21.686776159935846 26.3625965362213 27.47500276025313 24.475624543589724 96-97 21.369691003067523 26.254824104651746 27.660161242951343 24.71532364932939 98-99 21.45358319596635 26.422523084432754 27.209461566622828 24.914432152978073 100-101 21.528862476516633 25.625131505640002 27.95425528643593 24.891750731407434 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1674.0 1 1155.0 2 745.5 3 837.0 4 722.0 5 749.0 6 1264.0 7 2325.5 8 3450.0 9 3951.0 10 3571.0 11 3136.0 12 3152.0 13 3313.5 14 3580.0 15 3727.0 16 3808.0 17 3985.5 18 4240.0 19 4601.0 20 4979.5 21 5565.0 22 6534.0 23 7779.5 24 9094.5 25 10648.5 26 13112.0 27 16254.0 28 18588.5 29 20588.5 30 23216.0 31 27065.0 32 30690.0 33 32355.0 34 34204.5 35 37185.0 36 40497.0 37 43384.0 38 46206.0 39 48551.0 40 51130.5 41 54841.5 42 57157.0 43 59288.5 44 63078.0 45 66920.5 46 70314.5 47 78186.5 48 93634.5 49 94610.5 50 82852.5 51 78561.0 52 77556.5 53 76903.5 54 74781.0 55 69874.5 56 64257.0 57 58376.5 58 52984.5 59 47427.5 60 39893.0 61 32667.0 62 26034.5 63 19096.5 64 13118.0 65 9248.0 66 6566.5 67 4809.0 68 3581.0 69 2620.5 70 1842.0 71 1102.5 72 715.5 73 484.0 74 324.5 75 236.0 76 146.5 77 67.0 78 31.0 79 20.0 80 12.5 81 8.0 82 4.0 83 2.5 84 3.0 85 3.5 86 2.0 87 1.0 88 1.0 89 1.5 90 1.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.39888078999413085 2 0.0 3 0.0 4 0.0 5 2.421274675210215E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0011864245908530054 16-17 0.0021307217141849893 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0022275727011933978 36-37 0.0073122495191348495 38-39 0.012808543031862036 40-41 0.0025181256622186237 42-43 6.779569090588603E-4 44-45 2.9055296102522584E-4 46-47 3.6319120128153225E-4 48-49 4.600421882899409E-4 50-51 6.295314155546559E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 1.937019740168172E-4 60-61 0.0022033599544412957 62-63 0.0 64-65 1.6948922726471507E-4 66-67 0.0018159560064076614 68-69 0.002058083473928683 70-71 2.421274675210215E-5 72-73 0.0 74-75 0.0 76-77 0.12246807307213269 78-79 4.84254935042043E-5 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.1475524787073105 88-89 0.43883182213509936 90-91 0.3356128827308879 92-93 0.0 94-95 0.0 96-97 3.6319120128153225E-4 98-99 0.0 100-101 1.2106373376051076E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2065028.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.53076730228439 #Duplication Level Percentage of deduplicated Percentage of total 1 69.00810891212424 31.419921488486256 2 15.057405041302733 13.711504102235969 3 6.012105560669922 8.212073378388967 4 3.0485307481051063 5.552077764233301 5 1.8343453163335157 4.175957487500828 6 1.160167701727721 3.1693995395394565 7 0.7761582140523576 2.473735532364217 8 0.5280412424267744 1.9233698347954082 9 0.40083909098628784 1.6425460239620282 >10 2.0098847208828494 15.846359250755068 >50 0.10746547282861915 3.3199216071985864 >100 0.05056321116376146 4.121215937123882 >500 0.004043686017544865 1.2499275408265418 >1k 0.0019154302188370397 1.6529877475145012 >5k 3.1923836980617327E-4 1.0422199911124788 >10k+ 1.0641278993539109E-4 0.4867827739624877 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10047 0.4865309332367406 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8498 0.41151984379872814 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7527 0.3644986896061458 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5486 0.2656622573640648 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3695 0.1789321984980349 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2843 0.1376736780324528 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2428 0.11757709822820804 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2138 0.1035337051119888 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 4.8425493504204304E-5 0.0 0.0 7.263824025630646E-5 0.0 18-19 1.2106373376051077E-4 0.0 0.0 9.685098700840861E-5 0.0 20-21 1.4527648051261292E-4 0.0 0.0 2.4212746752102154E-4 0.0 22-23 1.4527648051261292E-4 0.0 0.0 4.358294415378387E-4 0.0 24-25 1.4527648051261292E-4 0.0 0.0 8.474461363235752E-4 0.0 26-27 1.4527648051261292E-4 0.0 0.0 0.0021065089674328873 0.0 28-29 1.6948922726471507E-4 0.0 0.0 0.007578589733407973 0.0 30-31 1.9370197401681721E-4 0.0 0.0 0.02205781229116506 0.0 32-33 1.9370197401681721E-4 0.0 0.0 0.051064682900183435 0.0 34-35 2.6634021427312366E-4 0.0 0.0 0.09750473117071537 0.0 36-37 2.9055296102522584E-4 0.0 0.0 0.1723947568749673 0.0 38-39 2.9055296102522584E-4 0.0 0.0 0.2782044601816537 0.0 40-41 2.9055296102522584E-4 0.0 0.0 0.3905031796179035 0.0 42-43 3.3897845452943013E-4 0.0 0.0 0.5102109995602966 0.0 44-45 3.8740394803363443E-4 0.0 0.0 0.6540589280145354 2.4212746752102152E-5 46-47 3.8740394803363443E-4 0.0 0.0 0.830303511623087 4.8425493504204304E-5 48-49 3.8740394803363443E-4 0.0 0.0 1.0151194075818826 4.8425493504204304E-5 50-51 3.8740394803363443E-4 0.0 0.0 1.2156251634360404 4.8425493504204304E-5 52-53 3.8740394803363443E-4 0.0 0.0 1.410537774790463 4.8425493504204304E-5 54-55 3.8740394803363443E-4 0.0 0.0 1.6282103680918611 4.8425493504204304E-5 56-57 4.358294415378387E-4 0.0 0.0 1.8915724145144763 4.8425493504204304E-5 58-59 4.358294415378387E-4 0.0 0.0 2.1691715560273277 4.8425493504204304E-5 60-61 4.358294415378387E-4 0.0 0.0 2.477835651623126 4.8425493504204304E-5 62-63 4.358294415378387E-4 0.0 0.0 2.7929645506017353 4.8425493504204304E-5 64-65 4.358294415378387E-4 0.0 0.0 3.1228147996056226 4.8425493504204304E-5 66-67 4.84254935042043E-4 0.0 0.0 3.500848414646194 4.8425493504204304E-5 68-69 4.84254935042043E-4 0.0 0.0 3.93895385437873 4.8425493504204304E-5 70-71 5.084676817941452E-4 0.0 0.0 4.399189744642687 4.8425493504204304E-5 72-73 5.326804285462473E-4 0.0 0.0 4.861435292887069 4.8425493504204304E-5 74-75 5.326804285462473E-4 0.0 0.0 5.345908142649882 4.8425493504204304E-5 76-77 5.326804285462473E-4 0.0 0.0 5.872317469787335 4.8425493504204304E-5 78-79 5.326804285462473E-4 0.0 0.0 6.467224657486485 4.8425493504204304E-5 80-81 6.295314155546559E-4 0.0 0.0 7.1148429948649605 4.8425493504204304E-5 82-83 6.779569090588603E-4 0.0 0.0 7.778926000034867 4.8425493504204304E-5 84-85 7.263824025630645E-4 0.0 0.0 8.453444699054927 4.8425493504204304E-5 86-87 7.748078960672689E-4 0.0 0.0 9.171594767722278 4.8425493504204304E-5 88-89 7.748078960672689E-4 0.0 0.0 9.92352161810881 4.8425493504204304E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACATGG 8985 0.0 23.185234 2 GTACATG 9135 0.0 23.045738 1 GAGTACT 8700 0.0 21.8742 12-13 ACATGGG 9545 0.0 20.830664 3 GTACTTT 9275 0.0 20.211115 14-15 AGTACTT 9165 0.0 19.573406 12-13 CATGGGG 3820 0.0 19.130325 4 GTATCAA 21185 0.0 18.840502 1 GGTATCA 17165 0.0 18.258244 1 AGAGTAC 16035 0.0 17.772608 10-11 TACTTTT 10050 0.0 17.755335 14-15 GTACGGA 135 0.009256486 17.640593 1 ACTTTTT 10950 0.0 17.18364 16-17 CATGGGA 5755 0.0 17.06829 4 GTGTAGC 1080 0.0 16.758562 1 GTATTAC 655 0.0 16.724895 1 CAACGCA 23965 0.0 16.514051 5 TATCAAC 24020 0.0 16.495995 2 TCAACGC 24140 0.0 16.394335 4 ATCAACG 24155 0.0 16.384155 3 >>END_MODULE