FastQCFastQC Report
Thu 26 May 2016
SRR938933_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938933_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1993714
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52970.26568504810619775No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45040.2259100352407617No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26650.13367012520351465No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA67650.031.0783651
GTATCAA97800.028.6794531
GTATACG1000.001662320923.72971
ATCAACG118100.023.5381163
TCAACGC118200.023.5182024
TATCAAC118550.023.4887162
CAACGCA120000.023.165435
TATAACG1452.390549E-522.8619632
AACGCAG123700.022.537216
GTACATG78900.022.2559951
TACATGG81500.021.0927162
GAGTACT56500.020.860112-13
ACGCAGA132100.020.6741457
CGTATAC1150.00378987820.5899683
TAAATCG1150.00378987820.5899685
CGCAGAG133800.020.1284688
ACATGGG84250.019.898343
AGTACTT58750.019.57779912-13
GTACTTT60450.019.4588614-15
CATGGGG45550.018.818014
Produced by FastQC (version 0.11.5)