##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938933_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1993714 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.81545246710411 33.0 31.0 34.0 30.0 34.0 2 31.90753488213455 33.0 31.0 34.0 30.0 34.0 3 31.952560397328803 34.0 31.0 34.0 30.0 34.0 4 35.48018421900032 37.0 35.0 37.0 33.0 37.0 5 35.51833562888157 37.0 35.0 37.0 33.0 37.0 6 35.59267778628229 37.0 35.0 37.0 33.0 37.0 7 35.51395034593728 37.0 35.0 37.0 33.0 37.0 8 35.50040928638712 37.0 35.0 37.0 33.0 37.0 9 37.11428369364914 39.0 37.0 39.0 33.0 39.0 10-11 37.00194611664462 39.0 37.0 39.0 33.0 39.0 12-13 36.894379785666345 39.0 37.0 39.0 33.0 39.0 14-15 38.13142180874489 40.0 38.0 41.0 33.0 41.0 16-17 38.03826928034813 40.0 38.0 41.0 33.0 41.0 18-19 37.94594585783116 40.0 38.0 41.0 33.0 41.0 20-21 38.055508212311295 40.0 38.0 41.0 33.0 41.0 22-23 37.99003919318418 40.0 38.0 41.0 33.0 41.0 24-25 37.9184524460379 40.0 38.0 41.0 32.5 41.0 26-27 37.80214263429961 40.0 38.0 41.0 32.0 41.0 28-29 37.67538674052547 40.0 38.0 41.0 32.0 41.0 30-31 37.4894031942395 40.0 37.0 41.0 32.0 41.0 32-33 37.293949633698716 40.0 37.0 41.0 31.0 41.0 34-35 36.98716816955692 40.0 36.5 41.0 30.5 41.0 36-37 36.72954947399677 40.0 36.0 41.0 30.0 41.0 38-39 36.49946933211082 39.5 36.0 41.0 30.0 41.0 40-41 36.345170370474406 39.0 35.5 41.0 30.0 41.0 42-43 36.13890583102692 39.0 35.0 41.0 29.5 41.0 44-45 36.077398513528024 39.0 35.0 41.0 29.0 41.0 46-47 35.880512701420564 39.0 35.0 41.0 27.5 41.0 48-49 35.65904462726349 39.0 35.0 41.0 27.0 41.0 50-51 34.55436010380626 38.0 34.0 40.0 25.0 40.5 52-53 34.64540701424578 38.0 34.5 39.5 25.0 40.5 54-55 35.13139823465151 39.0 35.0 40.5 26.0 41.0 56-57 35.03226214993725 39.0 35.0 41.0 26.0 41.0 58-59 34.821289312308586 38.5 34.5 40.0 25.0 41.0 60-61 34.51104922772273 38.0 34.0 40.0 24.5 41.0 62-63 34.13090593736113 37.5 34.0 40.0 23.0 41.0 64-65 33.74931409419806 37.0 33.5 40.0 22.0 41.0 66-67 33.346448638069454 36.5 33.0 39.5 22.0 41.0 68-69 32.91333536304605 36.0 32.5 39.0 20.5 41.0 70-71 32.48162148633154 35.5 32.0 39.0 19.5 40.0 72-73 32.04415879108036 35.0 32.0 38.0 18.0 40.0 74-75 31.57053970629689 35.0 31.0 37.0 15.0 39.0 76-77 31.142675679661174 35.0 31.0 37.0 11.0 39.0 78-79 30.74642300751261 35.0 31.0 36.0 7.5 39.0 80-81 30.307980984233446 34.5 30.5 36.0 4.5 37.5 82-83 29.84120891963441 34.0 30.0 35.0 2.0 37.0 84-85 29.434400320206407 34.0 29.5 35.0 2.0 36.5 86-87 28.987088418900605 34.0 29.0 35.0 2.0 36.0 88-89 28.52870772839033 34.0 29.0 35.0 2.0 36.0 90-91 28.08775757204895 34.0 28.0 35.0 2.0 35.5 92-93 27.606011694756617 34.0 27.0 35.0 2.0 35.0 94-95 27.08870931337193 33.5 26.0 35.0 2.0 35.0 96-97 26.454022241906312 33.0 25.0 35.0 2.0 35.0 98-99 25.608787669645697 33.0 22.0 35.0 2.0 35.0 100-101 22.397754392054225 28.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.022028289240282106 1101 2 0.21689234531590174 1101 3 0.2766103671238227 1101 4 0.3094630783244767 1101 5 0.21053543764386973 1101 6 0.17033839760308922 1101 7 0.171797384748416 1101 8 0.2713217136669144 1101 9 0.24086634614771896 1101 10-11 0.12677543332723928 1101 12-13 0.15677835754326708 1101 14-15 0.3119459926957546 1101 16-17 0.36085042601822437 1101 18-19 0.38648307837750906 1101 20-21 0.41755068543194085 1101 22-23 0.353908261754583 1101 24-25 0.3028749520285956 1101 26-27 0.3881692836325499 1101 28-29 0.4159313387850432 1101 30-31 0.3127899932816476 1101 32-33 0.26222893851328877 1101 34-35 0.2321373166753702 1101 36-37 0.22483953476263707 1101 38-39 0.38892065632794015 1101 40-41 0.09374379895785268 1101 42-43 0.14889880966700986 1101 44-45 0.28860074347491604 1101 46-47 0.2674175791177049 1101 48-49 0.30741671978407936 1101 50-51 0.14874188806577138 1101 52-53 0.16889511447461558 1101 54-55 0.25362871903612216 1101 56-57 0.3189966008456935 1101 58-59 0.25794885003173107 1101 60-61 0.3623003728905019 1101 62-63 0.29752557778882505 1101 64-65 0.2731512580565223 1101 66-67 0.24323504847372135 1101 68-69 0.12304090459633699 1101 70-71 0.1620910296285345 1101 72-73 -2.7210870126737063E-4 1101 74-75 -0.17336759703808724 1101 76-77 -0.1808031945462254 1101 78-79 -0.25525871639304043 1101 80-81 -0.3097626942473255 1101 82-83 -0.16342267317235937 1101 84-85 -0.17201770243786996 1101 86-87 -0.09768854209056954 1101 88-89 -0.2268899067006238 1101 90-91 -0.1602954877253424 1101 92-93 -0.35633880546420826 1101 94-95 -0.6606730560208227 1101 96-97 -0.6383485038862808 1101 98-99 -0.5970112321509227 1101 100-101 -0.12658016824641294 1102 1 0.3395442746532922 1102 2 0.2073691747578401 1102 3 0.4292207500604519 1102 4 0.1683077467845493 1102 5 0.3509638968209501 1102 6 0.29131796724542625 1102 7 0.33708657799333963 1102 8 0.3565860977414701 1102 9 0.45546742769629844 1102 10-11 0.509484572465432 1102 12-13 0.5623902750858818 1102 14-15 0.5770818425328912 1102 16-17 0.6164302920346358 1102 18-19 0.6211085999472488 1102 20-21 0.5708687508322967 1102 22-23 0.7152899520712097 1102 24-25 0.6730072363531718 1102 26-27 0.6910752496110533 1102 28-29 0.6257658544700746 1102 30-31 0.6491764737486747 1102 32-33 0.7157814722538163 1102 34-35 0.7666681485443192 1102 36-37 0.7063976022255289 1102 38-39 0.6975746562939591 1102 40-41 0.664396319983247 1102 42-43 0.6423597416691393 1102 44-45 0.6760364573127902 1102 46-47 0.7113506998289054 1102 48-49 0.8334096579011927 1102 50-51 0.85956915143381 1102 52-53 0.8235667269593634 1102 54-55 0.9734327535439533 1102 56-57 0.9609986317522754 1102 58-59 0.8179736826401438 1102 60-61 0.9352657894695113 1102 62-63 0.7535682572964078 1102 64-65 0.662436673088628 1102 66-67 0.711169312961502 1102 68-69 0.739325540899415 1102 70-71 0.6550385219628225 1102 72-73 0.611949689031924 1102 74-75 0.5336824789304764 1102 76-77 0.6688396975444313 1102 78-79 0.6572543869388525 1102 80-81 0.701632150671923 1102 82-83 0.6180263487873283 1102 84-85 0.5391586413091787 1102 86-87 0.1990187934900831 1102 88-89 0.4231494692145574 1102 90-91 0.4552874721129818 1102 92-93 0.3361269471577799 1102 94-95 0.2593449092404789 1102 96-97 0.3142637247963762 1102 98-99 0.21671593260153799 1102 100-101 0.1718258396275978 1103 1 0.1634251631536614 1103 2 0.13958486905567824 1103 3 0.2451759065523902 1103 4 0.32870326615365286 1103 5 0.20669709096767264 1103 6 0.31484406938484 1103 7 0.18863254909894067 1103 8 0.19997500497157716 1103 9 0.328973651569747 1103 10-11 0.30896140207963185 1103 12-13 0.3376807186385733 1103 14-15 0.5261606418391267 1103 16-17 0.4936282353897141 1103 18-19 0.47226979292219795 1103 20-21 0.4389424538626301 1103 22-23 0.4496416299828567 1103 24-25 0.5143443810436992 1103 26-27 0.4757110937282505 1103 28-29 0.4294653625120546 1103 30-31 0.4475286119991111 1103 32-33 0.40021719686892254 1103 34-35 0.30283496788226216 1103 36-37 0.376244597701195 1103 38-39 0.571926868942576 1103 40-41 0.4665320129791226 1103 42-43 0.40177198435603145 1103 44-45 0.32050437076910043 1103 46-47 0.4071693472698428 1103 48-49 0.3973229328629557 1103 50-51 0.30435581462856476 1103 52-53 0.41078407196700795 1103 54-55 0.47557547949357826 1103 56-57 0.3288256001655938 1103 58-59 0.45079798899328694 1103 60-61 0.5932709764182107 1103 62-63 0.4258657431026265 1103 64-65 0.2792647876990344 1103 66-67 0.38940819159481066 1103 68-69 0.3618782473660076 1103 70-71 0.3523242086813525 1103 72-73 0.43840428986239743 1103 74-75 0.26570311047809625 1103 76-77 0.2504178923653875 1103 78-79 0.15407822365343193 1103 80-81 0.15473600821799138 1103 82-83 0.25032492731694234 1103 84-85 0.07494707611079221 1103 86-87 0.023766014020264237 1103 88-89 0.10928850547902513 1103 90-91 0.15873309633703414 1103 92-93 0.08735277355890503 1103 94-95 0.019710088098374712 1103 96-97 -0.17193802681597248 1103 98-99 -0.1899306155550171 1103 100-101 0.12541154169075597 1104 1 0.19294720453785175 1104 2 0.23493402691434184 1104 3 0.3014317650009275 1104 4 0.25024488071369433 1104 5 0.33045447573354636 1104 6 0.21603062508237514 1104 7 0.2131861154614043 1104 8 0.23928959202613953 1104 9 0.3253981538995063 1104 10-11 0.3038061092196145 1104 12-13 0.39626307622579304 1104 14-15 0.4107450920130944 1104 16-17 0.43980015773250614 1104 18-19 0.3895703446660619 1104 20-21 0.3747944283158162 1104 22-23 0.4474839335985834 1104 24-25 0.27209824336419786 1104 26-27 0.3414734693816115 1104 28-29 0.3588381482170959 1104 30-31 0.29318544479416886 1104 32-33 0.27671244883044466 1104 34-35 0.4754398979440211 1104 36-37 0.3935932653226146 1104 38-39 0.5024299238468828 1104 40-41 0.3505160076409837 1104 42-43 0.14889652163789435 1104 44-45 0.14006103515858115 1104 46-47 0.1661444053152934 1104 48-49 0.09389927256105324 1104 50-51 0.04899583069745006 1104 52-53 0.04550370619555366 1104 54-55 0.10311815963873272 1104 56-57 0.16357593096224576 1104 58-59 0.16446298333059417 1104 60-61 0.10993748232035472 1104 62-63 0.27771224664437 1104 64-65 0.17653980057911411 1104 66-67 0.16613661831910065 1104 68-69 0.2619831887758508 1104 70-71 -0.0106932072236674 1104 72-73 0.09398482381245543 1104 74-75 -0.11810697479140941 1104 76-77 0.06421483655324067 1104 78-79 -0.07994863839698496 1104 80-81 0.02313610422278245 1104 82-83 0.13072406213899157 1104 84-85 0.12325388518835112 1104 86-87 -0.10197430663120599 1104 88-89 -0.07544497341240586 1104 90-91 0.03900328855088375 1104 92-93 -0.04014891163161849 1104 94-95 0.01042990843550129 1104 96-97 0.07090033901449289 1104 98-99 0.1306646822527604 1104 100-101 0.21767955280279594 1105 1 0.18989609979654176 1105 2 0.16545781767199585 1105 3 0.23341205828872091 1105 4 0.25409258483456654 1105 5 0.2206057229533087 1105 6 0.15857442120682208 1105 7 0.19792357449463793 1105 8 0.1938390783065529 1105 9 0.20081399673982503 1105 10-11 0.32629109508965826 1105 12-13 0.26371643390059774 1105 14-15 0.4402320404704767 1105 16-17 0.3535936773769066 1105 18-19 0.37106288223085926 1105 20-21 0.46516565260878195 1105 22-23 0.2992601705731417 1105 24-25 0.35971378957089684 1105 26-27 0.22402305895609942 1105 28-29 0.2518464027606413 1105 30-31 0.3818928766902516 1105 32-33 0.3277757883052814 1105 34-35 0.38995100261796267 1105 36-37 0.4140704727000539 1105 38-39 0.42109777278154326 1105 40-41 0.5432555853254186 1105 42-43 0.4629450204494532 1105 44-45 0.5008757839799642 1105 46-47 0.4583201040379308 1105 48-49 0.3229149979605026 1105 50-51 0.19252294146124882 1105 52-53 0.27534718760992405 1105 54-55 0.46000899689331476 1105 56-57 0.3077171923400357 1105 58-59 0.22633093870306453 1105 60-61 0.3411051207372111 1105 62-63 0.2713547492351367 1105 64-65 0.3453857475780211 1105 66-67 0.2500940667759153 1105 68-69 0.40680765060812973 1105 70-71 0.2323604068898959 1105 72-73 0.21130780079247558 1105 74-75 0.20605465909623177 1105 76-77 0.11308794876989836 1105 78-79 0.14708176374254833 1105 80-81 0.13667733932794945 1105 82-83 0.1059649225785968 1105 84-85 0.07681393777850687 1105 86-87 0.1627925350740007 1105 88-89 0.384124941515946 1105 90-91 0.4243975350711686 1105 92-93 0.23456489642778777 1105 94-95 0.413750026864399 1105 96-97 0.533931026335484 1105 98-99 0.548881882736449 1105 100-101 0.8036193772702447 1106 1 0.20895510429777886 1106 2 0.21354720388892545 1106 3 0.16416569880281884 1106 4 0.18857082958938065 1106 5 0.1732474389492893 1106 6 0.2751715662125136 1106 7 0.2645534985885547 1106 8 0.23382742351962804 1106 9 0.35683870745901203 1106 10-11 0.29083826162671755 1106 12-13 0.4640392436469156 1106 14-15 0.37659053014721167 1106 16-17 0.35043038824178296 1106 18-19 0.3299259299147934 1106 20-21 0.3604795820073434 1106 22-23 0.4118110799250374 1106 24-25 0.5312405452338567 1106 26-27 0.4404116389788584 1106 28-29 0.3292931581665073 1106 30-31 0.29135778485524355 1106 32-33 0.42901148494660646 1106 34-35 0.48996765996508174 1106 36-37 0.4667376835424406 1106 38-39 0.5150369086562208 1106 40-41 0.6085494402104672 1106 42-43 0.5181317219947772 1106 44-45 0.6448164195514892 1106 46-47 0.6545565076791959 1106 48-49 0.619604042246543 1106 50-51 0.5660160139330372 1106 52-53 0.6813127138642585 1106 54-55 0.6535871878732777 1106 56-57 0.7176587898599678 1106 58-59 0.6428837890911652 1106 60-61 0.7551151815606403 1106 62-63 0.723831230774941 1106 64-65 0.6766029015390487 1106 66-67 0.7489893793663001 1106 68-69 0.7218516452863497 1106 70-71 0.57640612836191 1106 72-73 0.5834024059544234 1106 74-75 0.7000732789037976 1106 76-77 0.5579079422997779 1106 78-79 0.6294718413197238 1106 80-81 0.6097395703198245 1106 82-83 0.7530251953948799 1106 84-85 0.8633685128179813 1106 86-87 0.8833084950620851 1106 88-89 0.8096275006414935 1106 90-91 0.830841893104509 1106 92-93 0.8572826791902735 1106 94-95 0.77803014024148 1106 96-97 1.0226926730883186 1106 98-99 1.1173066225926043 1106 100-101 1.2921809751758104 1107 1 0.08511881175109792 1107 2 0.11942443158707405 1107 3 0.09143618450887914 1107 4 0.1569479277852608 1107 5 0.1968696901339797 1107 6 0.14013643483504268 1107 7 0.18150627895062144 1107 8 0.19887009710965486 1107 9 0.1499686635111246 1107 10-11 0.13684969512367218 1107 12-13 0.29663113328197 1107 14-15 0.22722027381557552 1107 16-17 0.2130504886526623 1107 18-19 0.21096073598253895 1107 20-21 0.3421759893033922 1107 22-23 0.24551513510751022 1107 24-25 0.19059646307346867 1107 26-27 0.2516571504299634 1107 28-29 0.19714333750125945 1107 30-31 0.23502222270141004 1107 32-33 0.2809593027814472 1107 34-35 0.4371941874185339 1107 36-37 0.43220382957542114 1107 38-39 0.4104379202244033 1107 40-41 0.35195207625519487 1107 42-43 0.173204222549046 1107 44-45 0.14059827141331027 1107 46-47 0.314893016753679 1107 48-49 0.43604207696144925 1107 50-51 0.16713242715511 1107 52-53 0.3574036928393056 1107 54-55 0.3824674386725704 1107 56-57 0.22609120106802294 1107 58-59 0.33361992146942754 1107 60-61 0.40469911664787617 1107 62-63 0.1937227285879075 1107 64-65 0.36966618981939803 1107 66-67 0.3289026439218361 1107 68-69 0.3715894290776376 1107 70-71 0.07539937766726013 1107 72-73 0.3630094753282762 1107 74-75 0.38331796361237735 1107 76-77 0.5776435711731907 1107 78-79 0.6438823365985868 1107 80-81 0.5145950250592968 1107 82-83 0.3890354789116195 1107 84-85 0.4265754657433867 1107 86-87 0.46426347592683115 1107 88-89 0.4796816015123717 1107 90-91 0.7513663462422961 1107 92-93 0.5612029202924305 1107 94-95 0.5114756052789744 1107 96-97 0.577394952535581 1107 98-99 0.352356620831376 1107 100-101 0.34092640892555437 1108 1 0.2582062608380653 1108 2 0.26865552643358015 1108 3 0.20872251299221034 1108 4 0.22586728197457262 1108 5 0.16005397600216043 1108 6 0.28083568584351326 1108 7 0.3387808986603176 1108 8 0.2833114656433082 1108 9 0.36189855450853514 1108 10-11 0.4009706828827717 1108 12-13 0.3786711694529359 1108 14-15 0.494908438487073 1108 16-17 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-1.580782843732937 2316 78-79 -1.5577878059212544 2316 80-81 -1.5910488968189647 2316 82-83 -1.413229017594066 2316 84-85 -1.469985003534834 2316 86-87 -1.4230089839036069 2316 88-89 -1.473926959653486 2316 90-91 -1.6123832384728551 2316 92-93 -1.6229412505454022 2316 94-95 -1.5551200226697581 2316 96-97 -1.7818727259185536 2316 98-99 -1.583274544510747 2316 100-101 -1.490014298964983 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5998.0 3 1724.0 4 1791.0 5 2016.0 6 2327.0 7 2937.0 8 3639.0 9 4253.0 10 5484.0 11 6844.0 12 8784.0 13 12534.0 14 10969.0 15 11051.0 16 13598.0 17 18017.0 18 15664.0 19 11774.0 20 11835.0 21 12669.0 22 13950.0 23 15488.0 24 17670.0 25 19902.0 26 23534.0 27 27381.0 28 32194.0 29 37977.0 30 45743.0 31 55698.0 32 68714.0 33 88226.0 34 118740.0 35 169186.0 36 262645.0 37 404580.0 38 370676.0 39 57487.0 40 15.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.98263360468955 19.174245718288766 14.668283128407051 23.174837548614637 2 15.182378709548514 20.35403427990807 40.56900912370466 23.894577886838753 3 18.921104952403905 24.66276057356308 30.039412379001483 26.37672209503153 4 12.947703078729065 16.9910015032641 37.40059829308729 32.660697124919544 5 13.925278040569719 37.15047834828737 34.37624909864038 14.547994512502536 6 31.539323214914848 35.953994352930785 17.953885948473754 14.552796483680613 7 28.13768037022854 32.70977802247329 21.213109768185582 17.93943183911259 8 25.818341509931113 35.24942596519404 20.05761678314659 18.874615741728253 9 26.32668333307402 17.019560526813727 20.783454985392215 35.87030115472004 10-11 24.876918185443184 26.486373358166766 27.52879520977799 21.107913246612068 12-13 25.812731586155945 24.07961519872683 27.780100365920557 22.327552849196667 14-15 22.884578757621238 25.372909609616467 26.234146809061095 25.5083648237012 16-17 22.16876933017986 28.417939661608145 26.94131153505938 22.471979473152615 18-19 22.11797070846721 27.22957130284054 28.9090448693641 21.74341311932815 20-21 23.114235793744843 26.366166091705658 28.699614367048486 21.819983747501016 22-23 22.805966402901625 26.37398578053326 28.568728468426634 22.25131934813848 24-25 22.480494446116577 26.53345245479664 28.75646179130532 22.22959130778146 26-27 22.43928936476986 26.56535844024925 28.733104139347805 22.26224805563309 28-29 22.224210775740026 26.684926775491025 28.84780085503897 22.243061593729983 30-31 22.508972988168203 26.54041735062171 28.660552485078483 22.2900571761316 32-33 22.27757510535731 26.62159408272381 28.717873298170726 22.38295751374815 34-35 22.645826848882972 26.604157613598133 28.546838133776653 22.203177403742238 36-37 22.685218795941704 26.4263228689604 28.51425667459208 22.374201660505815 38-39 22.52394696725511 26.66380043139697 28.558439067761178 22.25381353358674 40-41 22.71686532208382 26.54228708426929 28.53015988600988 22.21068770763701 42-43 22.42123972853749 26.615494817199348 28.35808330892698 22.605182145336187 44-45 22.652509557132824 26.6372403265381 28.189723013293527 22.520527103035544 46-47 22.688856194628368 26.83739489645226 27.849469059940308 22.624279848979064 48-49 22.771154013896712 26.79425234212672 27.792968149295106 22.641625494681467 50-51 22.642323725091202 27.054187783220634 27.696078925662338 22.607409566025826 52-53 22.761770915393097 27.081367575232395 27.571980463210966 22.58488104616354 54-55 22.687868483869263 26.9001605638134 27.58193921764841 22.83003173466893 56-57 22.667117599117358 27.20551582455368 27.436106282292116 22.691260294036855 58-59 22.779252776004896 26.95180816696874 27.58883606219904 22.68010299482733 60-61 22.77589078964535 26.878230414263715 27.792531153773893 22.55334764231705 62-63 22.58126388276638 26.836759460822528 27.933594095572662 22.64838256083843 64-65 22.500782774030153 26.958031715331433 27.749755335846615 22.791430174791802 66-67 22.560469358451844 26.959923252732736 27.55496670040958 22.92464068840584 68-69 22.456444002998673 26.918744643204313 27.77389033165673 22.850921022140287 70-71 22.59116970250484 26.759132855890016 27.685240425372037 22.964457016233105 72-73 22.63497142718837 26.728197505116785 27.583314466318974 23.053516601375872 74-75 22.492200512015522 27.046763647402127 27.406041823509447 23.0549940170729 76-77 22.624328888664834 26.84954503062536 27.488770700476394 23.037355380233407 78-79 22.51597014940419 26.8255667550733 27.542345971348993 23.11611712417352 80-81 22.52473332367229 26.942846521708997 27.473212654287927 23.059207500330785 82-83 22.6234746668804 26.94234698275536 27.482133558362026 22.952044792002205 84-85 22.686034465810188 26.76809303802733 27.3858260174774 23.160046478685086 86-87 22.510497352315568 26.82999494130834 27.577884821631336 23.081622884744753 88-89 22.725279101832783 26.688411696944186 27.466393089315105 23.119916111907926 90-91 22.68347659961635 26.6876887468257 27.52096682744995 23.107867826108002 92-93 22.594522966193416 26.699865394889137 27.510092862147374 23.195518776770065 94-95 22.579199039866467 26.690048069710535 27.496353484455195 23.234399405967803 96-97 22.58660971555623 26.550690379264243 27.532502042762243 23.330197862417286 98-99 22.574359173471247 26.559006919002815 27.39196984209304 23.474664065432897 100-101 22.95928128528156 25.927021952297252 27.804394694183017 23.30930206823817 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1254.0 1 711.0 2 178.0 3 185.0 4 188.5 5 220.5 6 338.5 7 653.0 8 1045.5 9 1321.5 10 1446.5 11 1557.5 12 1764.0 13 1979.0 14 2207.5 15 2400.0 16 2579.0 17 2807.5 18 2941.5 19 3011.5 20 3292.5 21 3773.0 22 4355.0 23 5117.0 24 6197.5 25 7983.0 26 10501.0 27 13099.5 28 15515.0 29 18307.0 30 21660.0 31 25761.0 32 29865.5 33 33928.5 34 38721.0 35 43011.0 36 47800.5 37 52829.5 38 55548.5 39 57397.0 40 58834.5 41 60388.5 42 61972.0 43 64180.5 44 67628.5 45 70129.0 46 72422.5 47 75295.5 48 80305.0 49 80492.0 50 75785.0 51 73151.5 52 71060.0 53 69245.0 54 66859.0 55 63230.5 56 58171.0 57 52596.0 58 47837.0 59 42413.5 60 35696.0 61 28979.0 62 23212.0 63 18422.5 64 14025.5 65 10456.5 66 7842.0 67 6018.5 68 4459.0 69 3086.5 70 2123.0 71 1488.5 72 993.0 73 686.0 74 486.0 75 287.5 76 190.0 77 122.0 78 74.0 79 45.0 80 34.5 81 26.5 82 20.0 83 19.0 84 18.5 85 12.5 86 7.5 87 9.0 88 11.5 89 8.0 90 7.0 91 6.0 92 4.0 93 3.5 94 4.5 95 4.5 96 2.0 97 1.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.33394960360412773 2 0.08827745604434739 3 0.0980080392674175 4 0.10272285794251332 5 0.11425911640285417 6 0.058985391084177566 7 0.06094153925788754 8 0.06214532274940136 9 0.06435225915051006 10-11 0.0621202439266615 12-13 0.06043996280309011 14-15 0.058859996970478216 16-17 0.06026441104391102 18-19 0.06838994961162935 20-21 0.07047149189903867 22-23 0.086597174920776 24-25 0.09577602404356894 26-27 0.09058470773641555 28-29 0.0881771407533879 30-31 0.08145601625910236 32-33 0.07252795536370814 34-35 0.33046364724328564 36-37 0.5127114520939312 38-39 0.4862532941033669 40-41 0.4680460687942202 42-43 0.4986171537141235 44-45 0.5074448993185582 46-47 0.5023538983023643 48-49 0.5199843106884939 50-51 0.5155202802407969 52-53 0.5154450437725772 54-55 0.5055389087903279 56-57 0.4853755353074714 58-59 0.46859780289449743 60-61 0.4253869913136989 62-63 0.4972879761089103 64-65 0.5207868330161698 66-67 0.5307431256438988 68-69 0.5434831675957535 70-71 0.5412511523719049 72-73 0.5284107951290907 74-75 0.5302164703663615 76-77 0.5047865441081318 78-79 0.4865291611535054 80-81 0.49162016216969934 82-83 0.49992125249659675 84-85 0.5020278736067461 86-87 0.5015513759746885 88-89 0.5075452146095177 90-91 0.5217649071030248 92-93 0.5269311445874383 94-95 0.5345049490548794 96-97 0.5262790951962016 98-99 0.5292885539249862 100-101 0.5074699781412981 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1993714.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.469385656744016 #Duplication Level Percentage of deduplicated Percentage of total 1 78.20198739489513 48.070281223006425 2 12.528492925989582 15.402375267308857 3 3.9757143582543972 7.331541574457823 4 1.8020770328508406 4.430902724618771 5 0.969993378697629 2.981244853982635 6 0.602420986233134 2.2218268758288358 7 0.3906670573733318 1.680984480914667 8 0.2782478481553234 1.3682979429134978 9 0.1987073774783224 1.0992978377149691 >10 0.9848814169613066 10.51007151137451 >50 0.04640544719763651 1.9100113491155213 >100 0.019422076702947506 1.9926913627272513 >500 5.732412060016655E-4 0.23417360390240027 >1k 3.2756640342952E-4 0.4996576079826327 >5k 8.189160085738E-5 0.2666417841511188 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5297 0.26568504810619775 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4504 0.2259100352407617 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2665 0.13367012520351465 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 5.015764547974283E-5 0.0 0.0 0.0 3 0.0 5.015764547974283E-5 0.0 0.0 0.0 4 0.0 5.015764547974283E-5 0.0 5.015764547974283E-5 0.0 5 0.0 5.015764547974283E-5 0.0 5.015764547974283E-5 0.0 6 0.0 5.015764547974283E-5 0.0 5.015764547974283E-5 0.0 7 0.0 5.015764547974283E-5 0.0 1.0031529095948566E-4 0.0 8 0.0 5.015764547974283E-5 0.0 1.0031529095948566E-4 0.0 9 0.0 5.015764547974283E-5 0.0 1.0031529095948566E-4 0.0 10-11 0.0 5.015764547974283E-5 0.0 1.2539411369935708E-4 0.0 12-13 0.0 5.015764547974283E-5 0.0 3.00945872878457E-4 0.0 14-15 0.0 5.015764547974283E-5 0.0 3.511035183581998E-4 0.0 16-17 0.0 5.015764547974283E-5 0.0 5.015764547974283E-4 0.0 18-19 0.0 5.015764547974283E-5 0.0 7.523646821961425E-4 0.0 20-21 0.0 5.015764547974283E-5 0.0 8.025223276758853E-4 0.0 22-23 0.0 5.015764547974283E-5 0.0 0.001078389377814471 5.015764547974283E-5 24-25 0.0 5.015764547974283E-5 0.0 0.001329177605213185 1.0031529095948566E-4 26-27 0.0 5.015764547974283E-5 0.0 0.001880911705490356 1.0031529095948566E-4 28-29 0.0 5.015764547974283E-5 0.0 0.003862138701940198 1.0031529095948566E-4 30-31 0.0 5.015764547974283E-5 0.0 0.009128691477313195 1.0031529095948566E-4 32-33 0.0 5.015764547974283E-5 0.0 0.01928561468696112 1.504729364392285E-4 34-35 0.0 5.015764547974283E-5 0.0 0.03646460826377304 1.504729364392285E-4 36-37 0.0 5.015764547974283E-5 0.0 0.06670966848805797 1.504729364392285E-4 38-39 0.0 5.015764547974283E-5 0.0 0.10811480483158567 1.504729364392285E-4 40-41 0.0 5.015764547974283E-5 0.0 0.15200274462636065 1.504729364392285E-4 42-43 0.0 5.015764547974283E-5 0.0 0.1990756949090993 1.504729364392285E-4 44-45 0.0 5.015764547974283E-5 0.0 0.2559544648831277 1.504729364392285E-4 46-47 0.0 5.015764547974283E-5 0.0 0.3287582872969744 1.504729364392285E-4 48-49 0.0 5.015764547974283E-5 0.0 0.40670326837249476 1.504729364392285E-4 50-51 0.0 5.015764547974283E-5 0.0 0.4892376740094116 1.504729364392285E-4 52-53 0.0 5.015764547974283E-5 0.0 0.5765370559669041 1.7555175917909993E-4 54-55 0.0 5.015764547974283E-5 0.0 0.6774793174948863 2.0063058191897132E-4 56-57 0.0 5.015764547974283E-5 0.0 0.8040019782175377 2.0063058191897132E-4 58-59 0.0 5.015764547974283E-5 0.0 0.944167518510679 2.0063058191897132E-4 60-61 0.0 5.015764547974283E-5 0.0 1.0938630114449714 2.0063058191897132E-4 62-63 0.0 5.015764547974283E-5 0.0 1.245715283134893 2.0063058191897132E-4 64-65 0.0 5.015764547974283E-5 0.0 1.4111853555725644 2.0063058191897132E-4 66-67 0.0 5.015764547974283E-5 0.0 1.6061732023750648 2.0063058191897132E-4 68-69 0.0 5.015764547974283E-5 0.0 1.826917000131413 2.0063058191897132E-4 70-71 0.0 5.015764547974283E-5 0.0 2.06744798902952 2.0063058191897132E-4 72-73 0.0 5.015764547974283E-5 0.0 2.3248319468088203 2.0063058191897132E-4 74-75 0.0 5.015764547974283E-5 0.0 2.5826171657519588 2.0063058191897132E-4 76-77 0.0 5.015764547974283E-5 0.0 2.8779453823366845 2.0063058191897132E-4 78-79 0.0 5.015764547974283E-5 0.0 3.2036189744366546 2.0063058191897132E-4 80-81 0.0 5.015764547974283E-5 0.0 3.5658073324458774 2.0063058191897132E-4 82-83 0.0 5.015764547974283E-5 0.0 3.9390805301061236 2.0063058191897132E-4 84-85 0.0 5.015764547974283E-5 0.0 4.322636045089717 2.0063058191897132E-4 86-87 0.0 5.015764547974283E-5 0.0 4.7410762025044715 2.0063058191897132E-4 88-89 0.0 5.015764547974283E-5 0.0 5.201071969199193 2.0063058191897132E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6765 0.0 31.078365 1 GTATCAA 9780 0.0 28.679453 1 GTATACG 100 0.0016623209 23.7297 1 ATCAACG 11810 0.0 23.538116 3 TCAACGC 11820 0.0 23.518202 4 TATCAAC 11855 0.0 23.488716 2 CAACGCA 12000 0.0 23.16543 5 TATAACG 145 2.390549E-5 22.861963 2 AACGCAG 12370 0.0 22.53721 6 GTACATG 7890 0.0 22.255995 1 TACATGG 8150 0.0 21.092716 2 GAGTACT 5650 0.0 20.8601 12-13 ACGCAGA 13210 0.0 20.674145 7 CGTATAC 115 0.003789878 20.589968 3 TAAATCG 115 0.003789878 20.589968 5 CGCAGAG 13380 0.0 20.128468 8 ACATGGG 8425 0.0 19.89834 3 AGTACTT 5875 0.0 19.577799 12-13 GTACTTT 6045 0.0 19.45886 14-15 CATGGGG 4555 0.0 18.81801 4 >>END_MODULE