FastQCFastQC Report
Thu 26 May 2016
SRR938933_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938933_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1993714
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51660.25911439654835144No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47270.23709519018274436No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26960.13522501221338667No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA75200.028.3798471
GTATCAA100900.026.1086121
TAGGACG2858.54925E-1121.6438734
GAGTACT57200.021.61075812-13
TATCAAC121850.021.4956252
ATCAACG121900.021.4089553
TCAACGC123550.021.1998524
CAACGCA124950.020.8483945
AACGCAG126700.020.5978816
GTACTTT61950.019.8771614-15
GTACATG79300.019.8239691
AGTACTT60600.019.30201312-13
TACATGG80000.018.9206982
ACGCAGA139350.018.3194227
ACATGGG79350.018.238513
CGCAGAG140700.018.0087538
GTATAGC8200.017.428391
TAGCCCT13900.017.4097254
AGAGTAC118800.016.79607810-11
GCAGAGT147650.016.6790149
Produced by FastQC (version 0.11.5)