FastQCFastQC Report
Thu 26 May 2016
SRR938932_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938932_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1999777
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53830.2691800135715132No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46110.23057570919157488No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25890.12946443528453422No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA67400.032.7029381
GTATCAA95700.028.5796951
TATCAAC116450.023.3717482
ATCAACG115300.023.3174923
TCAACGC116000.023.2175854
CAACGCA116500.023.0773125
AACGCAG118800.022.7096466
GTACATG77550.022.2491421
ACGGTCG1100.002937568421.5149389
GAGTACT53100.021.08166112-13
TACATGG80700.021.056382
ACGCAGA129050.020.5391257
CGCAGAG131100.020.0740458
GTACTTT56000.019.52529514-15
AGTACTT56000.019.18695612-13
ACATGGG83200.019.1151963
GTCCTAC5250.018.9606691
CATGGGG45000.018.4072254
GCAGAGT135900.018.2504949
AGAGTAC108450.017.78528610-11
Produced by FastQC (version 0.11.5)