##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938932_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1999777 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.820413976158342 33.0 31.0 34.0 30.0 34.0 2 31.896823495819785 33.0 31.0 34.0 30.0 34.0 3 31.942598099688116 34.0 31.0 34.0 30.0 34.0 4 35.471647088650386 37.0 35.0 37.0 33.0 37.0 5 35.51094247008542 37.0 35.0 37.0 33.0 37.0 6 35.582062900013355 37.0 35.0 37.0 33.0 37.0 7 35.49099624608144 37.0 35.0 37.0 33.0 37.0 8 35.47224665550209 37.0 35.0 37.0 33.0 37.0 9 37.08896791992307 39.0 37.0 39.0 33.0 39.0 10-11 36.97789303507341 39.0 37.0 39.0 33.0 39.0 12-13 36.85619096529263 39.0 37.0 39.0 33.0 39.0 14-15 38.09021355881181 40.0 38.0 41.0 33.0 41.0 16-17 38.018259285910375 40.0 38.0 41.0 33.0 41.0 18-19 37.94595972450928 40.0 38.0 41.0 33.0 41.0 20-21 37.82560155457333 40.0 38.0 41.0 32.0 41.0 22-23 37.899374780288 40.0 38.0 41.0 32.5 41.0 24-25 37.84534850635846 40.0 38.0 41.0 32.0 41.0 26-27 37.766961266181184 40.0 38.0 41.0 32.0 41.0 28-29 37.63693276800363 40.0 38.0 41.0 32.0 41.0 30-31 37.46183324440675 40.0 37.0 41.0 31.5 41.0 32-33 37.27926088758896 40.0 37.0 41.0 31.0 41.0 34-35 36.975246990039395 40.0 36.5 41.0 30.5 41.0 36-37 36.72268407927484 40.0 36.0 41.0 30.0 41.0 38-39 36.52852743080854 39.5 36.0 41.0 30.0 41.0 40-41 36.36935293285201 39.0 35.5 41.0 30.0 41.0 42-43 36.163873771925566 39.0 35.0 41.0 29.5 41.0 44-45 36.098110439313984 39.0 35.0 41.0 29.0 41.0 46-47 35.88580501725942 39.0 35.0 41.0 27.5 41.0 48-49 35.71527375302347 39.0 35.0 41.0 27.0 41.0 50-51 34.56572407823472 38.0 34.0 40.0 25.0 40.5 52-53 34.68947712669963 38.5 34.5 39.5 25.5 40.5 54-55 35.180663894024185 39.0 35.0 40.5 26.0 41.0 56-57 35.079173577853936 39.0 35.0 41.0 26.0 41.0 58-59 34.87626770384898 38.5 34.5 40.5 26.0 41.0 60-61 34.608019294151305 38.0 34.0 40.0 25.0 41.0 62-63 34.25273668013983 38.0 34.0 40.0 23.5 41.0 64-65 33.851751970344694 37.0 34.0 40.0 22.5 41.0 66-67 33.4809928807062 36.5 33.0 39.5 22.0 41.0 68-69 33.08732923720995 36.0 33.0 39.0 21.5 41.0 70-71 32.68614250488929 35.5 32.5 39.0 20.5 40.5 72-73 32.245213591315434 35.0 32.0 38.0 19.5 40.0 74-75 31.779256887142914 35.0 31.5 37.0 18.0 39.5 76-77 31.364009837096837 35.0 31.0 37.0 15.5 39.0 78-79 30.93531303740367 35.0 31.0 36.0 11.5 39.0 80-81 30.508733223754447 34.5 31.0 36.0 7.5 37.5 82-83 30.123809804793233 34.0 30.0 35.0 4.5 37.0 84-85 29.775255440981667 34.0 30.0 35.0 2.0 37.0 86-87 29.381625301221085 34.0 30.0 35.0 2.0 36.0 88-89 28.978260075998474 34.0 29.0 35.0 2.0 36.0 90-91 28.567509277284415 34.0 29.0 35.0 2.0 35.5 92-93 28.15490677210509 34.0 29.0 35.0 2.0 35.0 94-95 27.69178738429335 34.0 27.5 35.0 2.0 35.0 96-97 27.119505824899477 34.0 26.5 35.0 2.0 35.0 98-99 26.427083619823613 33.0 25.0 35.0 2.0 35.0 100-101 23.786787226775786 31.0 12.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.14095853087595955 1101 2 0.24962016884745353 1101 3 0.2924725028938582 1101 4 0.1616599844290647 1101 5 -0.02324380785086788 1101 6 0.0981359624973166 1101 7 0.13515390351204104 1101 8 0.17339076953114585 1101 9 0.1308005864187365 1101 10-11 0.3254515143771002 1101 12-13 0.42756756828386955 1101 14-15 0.1636345382272566 1101 16-17 0.4153335784243737 1101 18-19 0.39210864377334786 1101 20-21 0.31954194414745274 1101 22-23 0.4149489877034114 1101 24-25 0.43244234766760314 1101 26-27 0.46288177225874705 1101 28-29 0.32299944949087234 1101 30-31 0.4219540828529418 1101 32-33 0.3387309631699438 1101 34-35 0.21119373055626056 1101 36-37 0.1674388866997205 1101 38-39 0.2557070949745963 1101 40-41 0.2533352564867215 1101 42-43 0.2370131219076299 1101 44-45 0.2888945728382666 1101 46-47 0.274331171753289 1101 48-49 0.3760171197261002 1101 50-51 0.46462404144568126 1101 52-53 0.4675294523582565 1101 54-55 0.533747798660734 1101 56-57 0.4819592493546594 1101 58-59 0.3709882100196964 1101 60-61 0.3764980970865963 1101 62-63 0.3020470076141635 1101 64-65 0.34156543637822523 1101 66-67 0.3455028777469593 1101 68-69 0.2793467153595657 1101 70-71 0.11989562464346193 1101 72-73 0.16777489904266218 1101 74-75 0.13826384335957442 1101 76-77 0.0733333155695739 1101 78-79 -0.07034477487937707 1101 80-81 -0.015123397642472725 1101 82-83 -0.016354723830602325 1101 84-85 -0.06434444301568831 1101 86-87 -0.08550548892633003 1101 88-89 0.011632816747280117 1101 90-91 -0.03660006632117074 1101 92-93 -0.06368931989841542 1101 94-95 -0.1988234182594404 1101 96-97 -0.14511555162076917 1101 98-99 -0.24301525509861932 1101 100-101 0.10487063652165673 1102 1 0.17578032236145447 1102 2 0.17814903545610505 1102 3 0.22127290404983313 1102 4 0.1901571440715415 1102 5 0.369549073027315 1102 6 0.27556674842501394 1102 7 0.24728482115633454 1102 8 0.1977182536043287 1102 9 0.3549048646243165 1102 10-11 0.14251503027458057 1102 12-13 0.2990726434936306 1102 14-15 0.40583329760490017 1102 16-17 0.3304499793065716 1102 18-19 0.40778962420302634 1102 20-21 0.46484631007098187 1102 22-23 0.4498652016960989 1102 24-25 0.43442214739690854 1102 26-27 0.44409504560491797 1102 28-29 0.5039613019598974 1102 30-31 0.4372808392023728 1102 32-33 0.49164011223599147 1102 34-35 0.5180966304167214 1102 36-37 0.4559067697778403 1102 38-39 0.2868589797232701 1102 40-41 0.31203309189265127 1102 42-43 0.24529213203570066 1102 44-45 0.5276761034606281 1102 46-47 0.5298087247577001 1102 48-49 0.4229713653168119 1102 50-51 0.3274148690533636 1102 52-53 0.33049473811903596 1102 54-55 0.33519109417385096 1102 56-57 0.42666509391230534 1102 58-59 0.4827675574707868 1102 60-61 0.37684072787084943 1102 62-63 0.45397603429411504 1102 64-65 0.35078978900438784 1102 66-67 0.3760140582936913 1102 68-69 0.37512759368443227 1102 70-71 0.44654665665633075 1102 72-73 0.45744401520051525 1102 74-75 0.4062610386200092 1102 76-77 0.2246414723612169 1102 78-79 0.13947572458216229 1102 80-81 0.17503487179969213 1102 82-83 0.13845502738843862 1102 84-85 0.13710709351506623 1102 86-87 0.21650923036924752 1102 88-89 0.19695374851304948 1102 90-91 0.19993767265087925 1102 92-93 0.060326681643637414 1102 94-95 0.04375442792965245 1102 96-97 -0.019356644687000824 1102 98-99 -0.04409007934843956 1102 100-101 0.006640220462006852 1103 1 -0.09834817846966715 1103 2 0.11573180380801418 1103 3 0.1637640917991945 1103 4 0.07987138519627734 1103 5 0.09047762797539605 1103 6 0.061683222267269855 1103 7 0.040627368594286395 1103 8 0.03284566143007339 1103 9 0.07989892803883691 1103 10-11 0.16818718367483143 1103 12-13 0.16466175206269185 1103 14-15 0.08689550572278648 1103 16-17 -0.018928180907686 1103 18-19 0.10853603404243728 1103 20-21 0.19624921925048255 1103 22-23 0.10605985321151223 1103 24-25 0.21988124614744464 1103 26-27 0.16954551034272214 1103 28-29 0.07048375965575104 1103 30-31 0.16807198535352086 1103 32-33 0.17635984645659875 1103 34-35 0.07829837888850477 1103 36-37 0.02779082026379598 1103 38-39 0.06072770231041602 1103 40-41 0.12625164918168252 1103 42-43 0.2269169263603814 1103 44-45 0.17958251929555757 1103 46-47 0.028119955654673845 1103 48-49 0.038819269716171334 1103 50-51 -0.11810776659971367 1103 52-53 -0.255581821659149 1103 54-55 -0.016814461235121314 1103 56-57 0.03303242568237863 1103 58-59 0.014931360074122324 1103 60-61 -0.036428284500267694 1103 62-63 -0.08621338628952913 1103 64-65 -0.16870115791328288 1103 66-67 -0.22862043610290783 1103 68-69 -0.2065254380160937 1103 70-71 -0.3090196992120795 1103 72-73 -0.28767184505423415 1103 74-75 -0.2548326291905596 1103 76-77 -0.3767029186461599 1103 78-79 -0.2959405409322535 1103 80-81 -0.3922106423591316 1103 82-83 -0.2467876877334909 1103 84-85 -0.26380736520026105 1103 86-87 -0.28009260073756437 1103 88-89 -0.4175257939853232 1103 90-91 -0.45416120981146335 1103 92-93 -0.4763885031596047 1103 94-95 -0.3298709648253002 1103 96-97 -0.43191944197239707 1103 98-99 -0.5601390697920827 1103 100-101 -0.22218974730921204 1104 1 0.15209805125848064 1104 2 0.1979677567369329 1104 3 0.08980048838606791 1104 4 0.21147605028048133 1104 5 0.24177276119863222 1104 6 0.15032288642672853 1104 7 0.15252077249265739 1104 8 0.21088229440105977 1104 9 0.2827340052521805 1104 10-11 0.24503199203407178 1104 12-13 0.24841012555396702 1104 14-15 0.2852308034518529 1104 16-17 0.2367852558874617 1104 18-19 0.31460443003397387 1104 20-21 0.36622772580631846 1104 22-23 0.3545654897687527 1104 24-25 0.35660701978558507 1104 26-27 0.358219701903586 1104 28-29 0.1731896463861844 1104 30-31 0.35586821787231315 1104 32-33 0.3848823691565926 1104 34-35 0.2822032036414228 1104 36-37 0.2936961331061312 1104 38-39 0.46534124164812596 1104 40-41 0.5132381566830446 1104 42-43 0.4310723620458461 1104 44-45 0.351415437315147 1104 46-47 0.37734328063795886 1104 48-49 0.3945970555193412 1104 50-51 0.37755743032685274 1104 52-53 0.29251386356476416 1104 54-55 0.34632652547156084 1104 56-57 0.4456140794300012 1104 58-59 0.42215770989853496 1104 60-61 0.35247583438343355 1104 62-63 0.3639459541112515 1104 64-65 0.34713247363612254 1104 66-67 0.4196487616247424 1104 68-69 0.4305770727990321 1104 70-71 0.19283307069535027 1104 72-73 0.24685528275843893 1104 74-75 0.23646575431420302 1104 76-77 0.27291126089851403 1104 78-79 0.2625508469160671 1104 80-81 0.4020604902162397 1104 82-83 0.34963842526905964 1104 84-85 0.4730391175464348 1104 86-87 0.4865404687429482 1104 88-89 0.5041454307360773 1104 90-91 0.4575734008715635 1104 92-93 0.48378679316867235 1104 94-95 0.406364858967855 1104 96-97 0.41400736431410934 1104 98-99 0.24764562805759027 1104 100-101 0.7705020439242212 1105 1 0.03496803814599048 1105 2 0.07529301890797768 1105 3 0.05910687025240691 1105 4 0.11621995780226513 1105 5 0.0315215032290439 1105 6 0.10255783619718528 1105 7 0.14728627520281634 1105 8 0.14602743989299682 1105 9 0.23874890036800878 1105 10-11 0.09942220879432995 1105 12-13 0.21750575223961022 1105 14-15 0.22115781654440525 1105 16-17 0.2770242727342307 1105 18-19 0.21428417664483135 1105 20-21 0.22793663274543263 1105 22-23 0.24650818535639019 1105 24-25 0.2314819577138465 1105 26-27 0.2604959628389807 1105 28-29 0.31558823456268925 1105 30-31 0.3186894817315036 1105 32-33 0.2723506508955822 1105 34-35 0.29172865541339377 1105 36-37 0.23809639682568928 1105 38-39 0.2733351357844569 1105 40-41 0.2073455153196022 1105 42-43 0.17026400700709132 1105 44-45 0.10462532007962011 1105 46-47 0.3562323305021522 1105 48-49 0.39496791114862617 1105 50-51 0.3307529827888871 1105 52-53 0.30374162928688975 1105 54-55 0.28864154646309004 1105 56-57 0.23450221593427045 1105 58-59 0.16725522419471162 1105 60-61 0.3550117205052956 1105 62-63 0.2736416897701872 1105 64-65 0.3073028940021558 1105 66-67 0.248213201267923 1105 68-69 0.17468957564671683 1105 70-71 0.09564081262812607 1105 72-73 0.16178709046572237 1105 74-75 0.19243340667471642 1105 76-77 0.09602756101389431 1105 78-79 0.10395875451343883 1105 80-81 0.06703691608133155 1105 82-83 0.010058549842955955 1105 84-85 0.09600833521690433 1105 86-87 0.1157990094457304 1105 88-89 0.2004207069224755 1105 90-91 0.13416078369640871 1105 92-93 0.06598157609870725 1105 94-95 0.19755686717339316 1105 96-97 0.20702425517389145 1105 98-99 0.45712499189714606 1105 100-101 0.7600558833429467 1106 1 0.08791309651239132 1106 2 0.1398630014427944 1106 3 0.08807621748272965 1106 4 -0.052358121710092576 1106 5 0.07801240783458496 1106 6 0.009675842818126057 1106 7 0.01121712610433434 1106 8 0.026234994761964003 1106 9 0.04539347998044718 1106 10-11 0.04929707697795038 1106 12-13 0.1358659694761073 1106 14-15 0.07636383280144798 1106 16-17 0.17061645834409944 1106 18-19 0.06396605893352358 1106 20-21 0.13715284056096522 1106 22-23 0.155426497217249 1106 24-25 0.08324148327316294 1106 26-27 0.10770003346307533 1106 28-29 0.09623622032574275 1106 30-31 0.003841773280612415 1106 32-33 0.027523748615280397 1106 34-35 0.1014454163534424 1106 36-37 -0.10556059149792674 1106 38-39 -0.11981447263234202 1106 40-41 -0.05172957980282433 1106 42-43 -0.057175287641491934 1106 44-45 0.05645952649980046 1106 46-47 -0.04044522453747845 1106 48-49 0.00776148218155015 1106 50-51 -0.056597351645869765 1106 52-53 -0.12382646915945372 1106 54-55 -0.13763838471053447 1106 56-57 -0.19831125620341084 1106 58-59 -0.01630932329047141 1106 60-61 0.05302745416975796 1106 62-63 0.022978793693980037 1106 64-65 -0.1955965490461864 1106 66-67 -0.08669205902642574 1106 68-69 0.01973260764828666 1106 70-71 0.10056385358726061 1106 72-73 0.008283528902374826 1106 74-75 -0.08845726602709902 1106 76-77 -0.2026470088600334 1106 78-79 0.06325406508290143 1106 80-81 -0.13256501010563326 1106 82-83 0.020133613417367968 1106 84-85 0.02614694992830735 1106 86-87 0.013340643767065785 1106 88-89 0.037126013866501495 1106 90-91 -0.13158606887250457 1106 92-93 0.04144808581833814 1106 94-95 0.1576816059326056 1106 96-97 0.2213293378684682 1106 98-99 0.1180747886710769 1106 100-101 0.4908325436086436 1107 1 0.26811191213841923 1107 2 0.11808780862055457 1107 3 0.23039595077833042 1107 4 0.19439137961840203 1107 5 0.21205644847042038 1107 6 0.2410876648956588 1107 7 0.21894380074145658 1107 8 0.29512374890978776 1107 9 0.3714300663258925 1107 10-11 0.3075692715523459 1107 12-13 0.362939906563426 1107 14-15 0.2974774210752429 1107 16-17 0.3507840503191275 1107 18-19 0.3912479636746937 1107 20-21 0.3555038482945818 1107 22-23 0.41808347703769755 1107 24-25 0.3310518113832117 1107 26-27 0.4435736245767927 1107 28-29 0.36189896239972796 1107 30-31 0.4095262656721559 1107 32-33 0.39966872792993513 1107 34-35 0.3784506354968329 1107 36-37 0.2757585701846139 1107 38-39 0.38791266841616334 1107 40-41 0.19710231879727047 1107 42-43 0.33157510297804293 1107 44-45 0.6665358521316378 1107 46-47 0.6809311088160683 1107 48-49 0.45666015534481375 1107 50-51 0.5540824584936033 1107 52-53 0.4722461137801517 1107 54-55 0.5342941960421186 1107 56-57 0.5417786679114585 1107 58-59 0.4989503616123656 1107 60-61 0.3708181463605271 1107 62-63 0.3221715910584493 1107 64-65 0.4078700794783927 1107 66-67 0.4230524408173295 1107 68-69 0.4540990681093007 1107 70-71 0.5002103914932903 1107 72-73 0.562024614614792 1107 74-75 0.55051078323838 1107 76-77 0.4741985680312517 1107 78-79 0.5373249606497659 1107 80-81 0.5496913275181647 1107 82-83 0.5206239931643317 1107 84-85 0.6715278966086764 1107 86-87 0.7338634442875822 1107 88-89 0.785437345538373 1107 90-91 0.7640773158127239 1107 92-93 0.5726225045013322 1107 94-95 0.6574134193867103 1107 96-97 0.555111721717779 1107 98-99 0.480135694742561 1107 100-101 0.581538674897228 1108 1 0.3505634682299821 1108 2 0.16269592623551787 1108 3 0.19622080134281106 1108 4 0.2967421474944274 1108 5 0.3732362353124401 1108 6 0.2993471778004775 1108 7 0.27183129218690283 1108 8 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68-69 -1.8499963741597867 2316 70-71 -1.78692318774943 2316 72-73 -1.8543576799089934 2316 74-75 -1.908385777182822 2316 76-77 -1.883036948010659 2316 78-79 -1.7267014378285381 2316 80-81 -1.83696220296304 2316 82-83 -2.0048687188799725 2316 84-85 -2.0310272490978925 2316 86-87 -2.1422805072871824 2316 88-89 -2.3917869259287805 2316 90-91 -2.4218569822420726 2316 92-93 -2.407063908479323 2316 94-95 -2.6603809625294907 2316 96-97 -2.9480739608909623 2316 98-99 -3.1733876403772925 2316 100-101 -3.935036879187077 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6478.0 3 1799.0 4 1724.0 5 1966.0 6 2260.0 7 2830.0 8 3550.0 9 4202.0 10 5226.0 11 6362.0 12 8509.0 13 11950.0 14 11758.0 15 10846.0 16 13236.0 17 17312.0 18 15144.0 19 11184.0 20 11251.0 21 12221.0 22 13208.0 23 14878.0 24 16642.0 25 19067.0 26 22378.0 27 25799.0 28 31015.0 29 36428.0 30 44342.0 31 54042.0 32 66980.0 33 86517.0 34 115903.0 35 164863.0 36 258376.0 37 408849.0 38 393761.0 39 66901.0 40 20.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.862143779232646 19.26132368532708 14.694686541773534 23.18184599366674 2 15.22493808722044 20.25964327828418 40.6577269351122 23.857691699383174 3 18.852523428384945 24.673494179129353 30.114415307696042 26.35956708478966 4 12.917106986937817 16.987793846512737 37.378973457635894 32.71612570891355 5 13.911864209928742 37.09326506317226 34.36337640934863 14.631494317550365 6 31.555461464428298 35.910729541561004 17.986269702206588 14.547539291804112 7 28.17587000503538 32.70596267409861 21.21327699310563 17.904890327760377 8 25.75802925407319 35.258495733614154 20.103966877950533 18.879508134362126 9 26.33458619331284 16.98468687760564 20.7912188680813 35.889508061000214 10-11 24.851661268663403 26.49656324622953 27.52881562753227 21.1229598575748 12-13 25.838020599361073 24.042164747338113 27.81647022903056 22.303344424270257 14-15 22.903590098358652 25.344911044712166 26.233637968672802 25.517860888256383 16-17 22.183162477886558 28.403330135867684 26.953709489766066 22.459797896479692 18-19 22.147044608748953 27.20843103878509 28.971396549104767 21.67312780336119 20-21 23.152801708386853 26.370393767952127 28.734142808085807 21.742661715575213 22-23 22.75618199418392 26.395825359399637 28.559169737961 22.288822908455444 24-25 22.48763219035411 26.5163585563211 28.76905243550713 22.22695681781766 26-27 22.388472997671567 26.6311309181042 28.74897974512406 22.231416339100175 28-29 22.25239449849279 26.68434778189542 28.818473744570305 22.24478397504149 30-31 22.506447768382493 26.540999746419903 28.70746994900612 22.24508253619149 32-33 22.303715062249783 26.62828401460413 28.72611346987285 22.341887453273237 34-35 22.647218807740956 26.66966042136637 28.542472367803096 22.140648403089575 36-37 22.663110272208055 26.43887832713966 28.587480210345102 22.31053119030718 38-39 22.509644090980764 26.68519157028863 28.55991382876762 22.245250509962982 40-41 22.66053493670714 26.602156630622265 28.581125296302286 22.15618313636831 42-43 22.449145867847154 26.633855277835615 28.332714724990872 22.58428412932636 44-45 22.58445448851932 26.709551009731353 28.209446628485768 22.496547873263562 46-47 22.699981440004187 26.8054877229903 27.90130709650745 22.59322374049806 48-49 22.803948164120786 26.77092839915116 27.8433966004212 22.58172683630685 50-51 22.61745999566365 27.096879186376015 27.68673846629041 22.598922351669923 52-53 22.7327024653755 27.11050284222608 27.55325573796635 22.60353895443207 54-55 22.711170411431603 26.912609296540868 27.58532141011244 22.79089888191509 56-57 22.699568834162577 27.239004868844923 27.432888690420516 22.628537606571985 58-59 22.824428920370046 26.93459313837029 27.585053811157472 22.655924130102193 60-61 22.73528324555269 26.94443194962491 27.787342248017115 22.53294255680529 62-63 22.531415919411074 26.892474665067873 27.9360002494764 22.640109166044653 64-65 22.503324540010958 26.960019481829622 27.772248757605688 22.764407220553736 66-67 22.531864882531817 26.96675605456671 27.571244035005705 22.93013502789577 68-69 22.422329257973523 26.87274193942038 27.806046387694362 22.898882414911736 70-71 22.588325515639454 26.75847791852674 27.6887763612864 22.9644202045474 72-73 22.57813016919031 26.76779861897474 27.602567427940468 23.05150378389448 74-75 22.47965228827952 27.07477729026064 27.39990797523633 23.0456624462235 76-77 22.632727770296203 26.851915665541664 27.511599232737733 23.003757331424403 78-79 22.528374621703144 26.869657165920025 27.55356507460322 23.04840313777361 80-81 22.53677382107271 27.003921166301808 27.42050422467071 23.03880078795477 82-83 22.6132430118733 26.944538613128334 27.46434136522349 22.97787700977488 84-85 22.642432925064053 26.833923180450963 27.38602611893843 23.137617775546552 86-87 22.470830967234413 26.887568512081312 27.606275774550546 23.035324746133725 88-89 22.678646256994256 26.690254597734974 27.492717119384913 23.138382025885857 90-91 22.651944542484046 26.745499730702054 27.508615395993765 23.093940330820136 92-93 22.587302417930545 26.77311887579866 27.51539446976505 23.124184236505748 94-95 22.60564515946432 26.732101602102627 27.456281877077288 23.20597136135577 96-97 22.54742064734948 26.606203910481714 27.548692342583376 23.297683099585434 98-99 22.539133847570454 26.575417251598815 27.381894022873993 23.503554877956738 100-101 22.85588021944744 26.091955237230003 27.736477955150708 23.315686588171854 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1812.0 1 1007.5 2 217.0 3 215.5 4 185.5 5 218.0 6 359.0 7 675.5 8 1090.5 9 1422.0 10 1537.0 11 1605.0 12 1823.0 13 2049.5 14 2255.0 15 2520.0 16 2697.0 17 2796.0 18 2998.0 19 3177.0 20 3395.5 21 3830.5 22 4385.5 23 5157.5 24 6313.5 25 8128.5 26 10667.5 27 13399.0 28 15630.0 29 18108.5 30 21747.5 31 26072.5 32 29909.0 33 33873.0 34 38779.0 35 43016.0 36 47785.5 37 53115.0 38 56128.0 39 57646.5 40 59036.5 41 60488.0 42 61964.5 43 64197.5 44 67582.5 45 70433.0 46 73063.5 47 76109.0 48 80879.5 49 80994.5 50 76514.5 51 73372.0 52 70888.0 53 69289.5 54 66745.5 55 63054.5 56 57896.5 57 52283.0 58 47668.5 59 42326.5 60 35763.0 61 28971.0 62 23051.5 63 18331.0 64 13879.0 65 10354.5 66 7866.5 67 6098.5 68 4531.0 69 2994.0 70 2018.5 71 1391.5 72 942.5 73 663.5 74 435.5 75 273.0 76 192.0 77 113.0 78 74.5 79 51.5 80 32.5 81 22.5 82 18.5 83 16.0 84 10.0 85 5.5 86 6.5 87 7.5 88 7.0 89 6.0 90 3.5 91 2.0 92 3.5 93 3.0 94 2.0 95 2.5 96 1.5 97 0.5 98 1.0 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.29298266756743374 2 0.09026006399713568 3 0.09401048216876182 4 0.108912143704023 5 0.11116239460699869 6 0.08570955661556263 7 0.09576067731552068 8 0.09166022011454278 9 0.09741086131103618 10-11 0.09081012532897419 12-13 0.09498559089338461 14-15 0.10441164189807163 16-17 0.09203526193170539 18-19 0.10318650529534043 20-21 0.10516172553239687 22-23 0.11918828949427861 24-25 0.1328648144268086 26-27 0.13636520472032632 28-29 0.12713917601812602 30-31 0.11903827276741356 32-33 0.11301260090500091 34-35 0.39266878256925647 36-37 0.599866885157695 38-39 0.5821899141754305 40-41 0.5444357045810608 42-43 0.572213801838905 44-45 0.5936661937806066 46-47 0.5815648444801595 48-49 0.6060175709591619 50-51 0.5965665171666641 52-53 0.599391832189289 54-55 0.589040678035601 56-57 0.5608125305971615 58-59 0.5555369423690741 60-61 0.503231110268795 62-63 0.5807147496945909 64-65 0.6142684909367394 66-67 0.6279700186570802 68-69 0.629595199864785 70-71 0.6256447593906721 72-73 0.6170938059593645 74-75 0.6134934045146033 76-77 0.5965665171666641 78-79 0.571288698689904 80-81 0.5669632163986285 82-83 0.581014783148321 84-85 0.5916909735435502 86-87 0.5812648110264295 88-89 0.5948663275955269 90-91 0.6101430309479506 92-93 0.6188189983183124 94-95 0.617943900744933 96-97 0.6136934268170902 98-99 0.618418953713339 100-101 0.5938162105074716 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1999777.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.57991847266489 #Duplication Level Percentage of deduplicated Percentage of total 1 78.40681269942259 48.2828513373195 2 12.323301786803412 15.177358386907997 3 4.026125451850845 7.437854312570883 4 1.7338931951025909 4.27092006378904 5 0.9733556455483244 2.996958064888696 6 0.6245288656283962 2.3075061977533515 7 0.39960569426374226 1.722538025178173 8 0.2668393425346167 1.3145555966864957 9 0.196419820550163 1.0885964882305053 >10 0.9851337065490524 10.560909271223146 >50 0.0433044257241961 1.8166952469404858 >100 0.019703093219158113 2.021377212833527 >500 5.694924684624706E-4 0.23771975350000651 >1k 3.254242676928314E-4 0.49447712687987666 >5k 8.135606692320785E-5 0.2696829152983157 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5383 0.2691800135715132 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4611 0.23057570919157488 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2589 0.12946443528453422 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 5.0005575621681816E-5 0.0 10-11 0.0 0.0 0.0 5.0005575621681816E-5 0.0 12-13 0.0 0.0 0.0 1.5001672686504544E-4 0.0 14-15 0.0 0.0 0.0 1.7501951467588635E-4 0.0 16-17 0.0 0.0 0.0 3.250362415409318E-4 0.0 18-19 0.0 0.0 0.0 4.5005018059513635E-4 0.0 20-21 0.0 0.0 0.0 5.750641196493409E-4 0.0 22-23 0.0 0.0 0.0 7.750864221360682E-4 5.0005575621681816E-5 24-25 0.0 0.0 0.0 9.251031490011136E-4 1.0001115124336363E-4 26-27 0.0 0.0 0.0 0.0015001672686504545 1.0001115124336363E-4 28-29 0.0 0.0 0.0 0.0034503847178960455 1.0001115124336363E-4 30-31 0.0 0.0 0.0 0.00992610676090384 1.0001115124336363E-4 32-33 0.0 0.0 0.0 0.021277372427025612 1.0001115124336363E-4 34-35 0.0 0.0 0.0 0.03922937407520939 1.0001115124336363E-4 36-37 0.0 0.0 0.0 0.06790757169424391 1.0001115124336363E-4 38-39 0.0 0.0 0.0 0.10996226079207833 1.0001115124336363E-4 40-41 0.0 0.0 0.0 0.15699250466427006 1.0001115124336363E-4 42-43 0.0 0.0 0.0 0.20774816392027712 1.0001115124336363E-4 44-45 0.0 0.0 0.0 0.27068018084016365 1.0001115124336363E-4 46-47 0.0 0.0 0.0 0.34468843276025274 1.0001115124336363E-4 48-49 0.0 0.0 0.0 0.429672908529301 1.0001115124336363E-4 50-51 0.0 0.0 0.0 0.5167576184744599 1.0001115124336363E-4 52-53 0.0 0.0 0.0 0.6062675988372703 1.0001115124336363E-4 54-55 0.0 0.0 0.0 0.7086790177104747 1.0001115124336363E-4 56-57 0.0 0.0 0.0 0.8329678759181649 1.0001115124336363E-4 58-59 0.0 0.0 0.0 0.975633783166823 1.0001115124336363E-4 60-61 0.0 0.0 0.0 1.129050889174143 1.0001115124336363E-4 62-63 0.0 0.0 0.0 1.2825430035448953 1.0001115124336363E-4 64-65 0.0 0.0 0.0 1.4487365341235547 1.0001115124336363E-4 66-67 0.0 0.0 0.0 1.6400078608764876 1.0001115124336363E-4 68-69 0.0 0.0 0.0 1.863757808995703 1.0001115124336363E-4 70-71 0.0 0.0 0.0 2.1115104334133257 1.0001115124336363E-4 72-73 0.0 0.0 0.0 2.3684640837453377 1.0001115124336363E-4 74-75 0.0 0.0 0.0 2.636668988592228 1.0001115124336363E-4 76-77 0.0 0.0 0.0 2.929476636644986 1.0001115124336363E-4 78-79 0.0 2.5002787810840908E-5 0.0 3.261863697802305 1.0001115124336363E-4 80-81 0.0 5.0005575621681816E-5 0.0 3.6284295698970435 1.0001115124336363E-4 82-83 0.0 5.0005575621681816E-5 0.0 4.011097237341963 1.0001115124336363E-4 84-85 0.0 5.0005575621681816E-5 0.0 4.39959055434681 1.0001115124336363E-4 86-87 0.0 5.0005575621681816E-5 0.0 4.816912085697555 1.0001115124336363E-4 88-89 0.0 5.0005575621681816E-5 0.0 5.2702126287081015 1.0001115124336363E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6740 0.0 32.702938 1 GTATCAA 9570 0.0 28.579695 1 TATCAAC 11645 0.0 23.371748 2 ATCAACG 11530 0.0 23.317492 3 TCAACGC 11600 0.0 23.217585 4 CAACGCA 11650 0.0 23.077312 5 AACGCAG 11880 0.0 22.709646 6 GTACATG 7755 0.0 22.249142 1 ACGGTCG 110 0.0029375684 21.514938 9 GAGTACT 5310 0.0 21.081661 12-13 TACATGG 8070 0.0 21.05638 2 ACGCAGA 12905 0.0 20.539125 7 CGCAGAG 13110 0.0 20.074045 8 GTACTTT 5600 0.0 19.525295 14-15 AGTACTT 5600 0.0 19.186956 12-13 ACATGGG 8320 0.0 19.115196 3 GTCCTAC 525 0.0 18.960669 1 CATGGGG 4500 0.0 18.407225 4 GCAGAGT 13590 0.0 18.250494 9 AGAGTAC 10845 0.0 17.785286 10-11 >>END_MODULE