FastQCFastQC Report
Thu 26 May 2016
SRR938932_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938932_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1999777
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52430.26217923298447776No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48240.2412268967989931No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28050.1402656396188175No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA73900.028.43231
GTATCAA102000.026.5784681
GTACATG78050.022.1590731
ATCAACG123750.021.626643
TCAACGC124000.021.5830364
TATCAAC124900.021.50352
TACATGG79850.021.3341162
CAACGCA125900.021.257325
GAGTACT60800.020.99599512-13
AACGCAG128500.020.8272116
AGTACTT63100.020.15548112-13
GTACTTT63150.020.1019514-15
ACATGGG81650.019.9339373
CATGGGG43100.019.5973744
TAGGACG2451.7764069E-719.3681934
TATACCG1507.7764044E-418.980835
ACGCAGA138100.018.8647
CGCAGAG139050.018.3256118
AGAGTAC119050.017.39973310-11
GCAGAGT148050.016.7949249
Produced by FastQC (version 0.11.5)