FastQCFastQC Report
Thu 26 May 2016
SRR938931_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938931_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2930984
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108080.3687498805861786No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84550.2884696743482735No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT64570.22030144142717942No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52200.17809718510916472No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT36480.12446332016824382No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA34540.11784438263736684No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG122100.023.5196821
TACATGG120250.022.9639782
GAGTACT98450.021.71413412-13
ACATGGG124350.021.2926463
GTATCAA232100.021.1464351
ACCGGTC1150.00379524820.585338
AGTACTT103150.020.5640812-13
GTACTTT107450.019.94010514-15
CATGGGA73000.019.011154
GGTATCA182400.018.6328771
ATCAACG265350.018.2251153
TATCAAC268150.018.176122
TCAACGC266550.018.1430664
CAACGCA267300.018.074445
AACGCAG271800.017.8721246
GTATAGG8300.017.7286361
AGAGTAC202550.016.7947210-11
TACTTTT127200.016.58348814-15
ACGCAGA289150.016.5544077
ATGGGAT31700.016.4360355
Produced by FastQC (version 0.11.5)