##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938931_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2930984 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.767065599812213 33.0 31.0 34.0 30.0 34.0 2 31.844901234534206 33.0 31.0 34.0 30.0 34.0 3 31.88670698987098 34.0 31.0 34.0 30.0 34.0 4 35.42071570503285 37.0 35.0 37.0 33.0 37.0 5 35.462297303567674 37.0 35.0 37.0 33.0 37.0 6 35.550384785450895 37.0 35.0 37.0 33.0 37.0 7 35.47838746304995 37.0 35.0 37.0 33.0 37.0 8 35.463847977334574 37.0 35.0 37.0 33.0 37.0 9 37.062615149042095 39.0 37.0 39.0 33.0 39.0 10-11 36.9479533153371 39.0 37.0 39.0 33.0 39.0 12-13 36.836068023571606 39.0 37.0 39.0 33.0 39.0 14-15 38.03170436959056 40.0 38.0 41.0 33.0 41.0 16-17 37.9468560729093 40.0 38.0 41.0 33.0 41.0 18-19 37.871873746154876 40.0 38.0 41.0 32.5 41.0 20-21 37.991046010486585 40.0 38.0 41.0 33.0 41.0 22-23 37.92777886197946 40.0 38.0 41.0 32.5 41.0 24-25 37.847319705600576 40.0 38.0 41.0 32.0 41.0 26-27 37.7241081834633 40.0 38.0 41.0 32.0 41.0 28-29 37.59435380745852 40.0 37.0 41.0 32.0 41.0 30-31 37.40444966605072 40.0 37.0 41.0 31.5 41.0 32-33 37.20090590736763 40.0 36.5 41.0 31.0 41.0 34-35 36.87946982992742 40.0 36.0 41.0 30.0 41.0 36-37 36.61124762195904 39.5 36.0 41.0 30.0 41.0 38-39 36.36759156651827 39.0 35.5 41.0 30.0 41.0 40-41 36.22039697248432 39.0 35.0 41.0 30.0 41.0 42-43 36.004500877521 39.0 35.0 41.0 29.0 41.0 44-45 35.9228898895388 39.0 35.0 41.0 28.0 41.0 46-47 35.70819885062491 39.0 35.0 41.0 27.0 41.0 48-49 35.4595431090719 39.0 35.0 41.0 26.0 41.0 50-51 34.35862290616394 38.0 34.0 40.0 24.0 40.5 52-53 34.455997200940025 38.0 34.0 39.5 24.0 40.5 54-55 34.94578254265462 39.0 35.0 40.0 24.5 41.0 56-57 34.85369998607976 39.0 34.5 40.5 24.5 41.0 58-59 34.645550265712814 38.0 34.0 40.0 24.0 41.0 60-61 34.333636928758395 38.0 34.0 40.0 23.0 41.0 62-63 33.958125155237965 37.5 34.0 40.0 22.5 41.0 64-65 33.57241919437295 37.0 33.0 40.0 21.5 41.0 66-67 33.16364640680399 36.5 33.0 39.5 20.5 41.0 68-69 32.71985039836451 36.0 32.0 39.0 18.5 41.0 70-71 32.275007642484574 35.5 32.0 39.0 17.5 40.0 72-73 31.832979640966993 35.0 31.5 38.0 13.5 40.0 74-75 31.362232785985867 35.0 31.0 37.0 9.5 39.5 76-77 30.924501123172284 35.0 31.0 37.0 7.5 39.0 78-79 30.513632111263657 35.0 30.5 36.0 4.5 39.0 80-81 30.052222905345097 34.0 30.0 36.0 2.0 37.5 82-83 29.575245719526272 34.0 29.5 35.0 2.0 37.0 84-85 29.151602499365403 34.0 29.0 35.0 2.0 37.0 86-87 28.698437794269772 34.0 29.0 35.0 2.0 36.0 88-89 28.211606750497445 34.0 28.5 35.0 2.0 36.0 90-91 27.742873212545682 34.0 27.0 35.0 2.0 35.5 92-93 27.238335828513563 33.5 26.0 35.0 2.0 35.0 94-95 26.69693437425793 33.0 25.0 35.0 2.0 35.0 96-97 26.040992206030467 33.0 24.5 35.0 2.0 35.0 98-99 25.170889366847447 32.5 18.5 35.0 2.0 35.0 100-101 21.9105392591703 28.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.20340862581905483 1101 2 0.29261121340140406 1101 3 0.3094559127973113 1101 4 0.16495453830673057 1101 5 0.16681348027033494 1101 6 0.2253451243223168 1101 7 0.33096062132441517 1101 8 0.2540828682101832 1101 9 0.2838698824904924 1101 10-11 0.29872554995564116 1101 12-13 0.40267633489999355 1101 14-15 0.45016746650403405 1101 16-17 0.4393380276962233 1101 18-19 0.574308247244403 1101 20-21 0.5464177289717256 1101 22-23 0.5486209642728923 1101 24-25 0.49103885206258724 1101 26-27 0.5155132873919968 1101 28-29 0.47661684510464397 1101 30-31 0.4243643600551579 1101 32-33 0.35113077496546197 1101 34-35 0.3323440809806826 1101 36-37 0.3537553272426237 1101 38-39 0.48896824643134096 1101 40-41 0.3823974010939324 1101 42-43 0.3518219689109685 1101 44-45 0.43933750009879446 1101 46-47 0.4924823230620774 1101 48-49 0.34488995312886317 1101 50-51 0.3123675768363796 1101 52-53 0.4289567198582134 1101 54-55 0.5260987667932469 1101 56-57 0.5626055789381397 1101 58-59 0.39007002942814495 1101 60-61 0.4949017804651987 1101 62-63 0.3932453965236604 1101 64-65 0.3867603841285785 1101 66-67 0.36636288910772663 1101 68-69 0.42126769125029995 1101 70-71 0.3805895538478907 1101 72-73 0.32292649856180944 1101 74-75 0.10639406619371528 1101 76-77 0.10784578912531018 1101 78-79 0.14868560403399655 1101 80-81 0.025884374097007168 1101 82-83 0.10585170163413338 1101 84-85 0.21178571010463898 1101 86-87 0.1596467184794932 1101 88-89 0.17684367004788015 1101 90-91 0.17004984379079247 1101 92-93 0.1742556320465809 1101 94-95 -0.012055249885058572 1101 96-97 0.08558028728964118 1101 98-99 -0.07044718636473846 1101 100-101 0.15633641137548437 1102 1 0.2627741597728992 1102 2 0.23777423592715152 1102 3 0.38103023674950975 1102 4 0.20846943614227342 1102 5 0.30293040487241285 1102 6 0.23172145301097657 1102 7 0.341087569608824 1102 8 0.4049103727538892 1102 9 0.42391642101557636 1102 10-11 0.3957039880236053 1102 12-13 0.45933354699450746 1102 14-15 0.5222746427996725 1102 16-17 0.5600807164663948 1102 18-19 0.647380616027796 1102 20-21 0.48165895824968885 1102 22-23 0.47276385682122424 1102 24-25 0.6136637350278349 1102 26-27 0.613443149153305 1102 28-29 0.5551051691669926 1102 30-31 0.5831151173388491 1102 32-33 0.6195720097509465 1102 34-35 0.48826603945400393 1102 36-37 0.41118634558265654 1102 38-39 0.45036054692150884 1102 40-41 0.4394972799285455 1102 42-43 0.2898086957931696 1102 44-45 0.501299828677304 1102 46-47 0.6670761436271491 1102 48-49 0.5370809813824238 1102 50-51 0.37429892562748535 1102 52-53 0.40860299952996826 1102 54-55 0.5853720498245849 1102 56-57 0.47712339575810603 1102 58-59 0.4722153452842903 1102 60-61 0.49658501558113244 1102 62-63 0.4006020633739098 1102 64-65 0.24902975682230988 1102 66-67 0.3236644807803586 1102 68-69 0.4286911367534145 1102 70-71 0.21832086959387453 1102 72-73 0.2512158635450099 1102 74-75 0.17990084044055976 1102 76-77 0.1863251634712917 1102 78-79 0.04963496240817733 1102 80-81 0.08699097210328688 1102 82-83 0.01913721135490576 1102 84-85 0.034259136331439066 1102 86-87 0.02118772194922869 1102 88-89 -0.06671253417087541 1102 90-91 0.09957358119234527 1102 92-93 0.1761467759580171 1102 94-95 -0.01894033552696328 1102 96-97 -0.18877946377055466 1102 98-99 -0.15762017749763402 1102 100-101 0.17717617014620402 1103 1 0.3264188449039871 1103 2 0.29915449036557007 1103 3 0.3906329302230134 1103 4 0.27473469449977017 1103 5 0.14493425817293115 1103 6 0.281004919994821 1103 7 0.22743726622307747 1103 8 0.21382298011736367 1103 9 0.4829490671861194 1103 10-11 0.4485085248327607 1103 12-13 0.412549792246395 1103 14-15 0.47465376810593796 1103 16-17 0.6028809916238416 1103 18-19 0.6303390793118169 1103 20-21 0.6137845495367316 1103 22-23 0.5661367143477705 1103 24-25 0.5624521104478291 1103 26-27 0.6147925580032805 1103 28-29 0.6187574594964644 1103 30-31 0.5762326868544747 1103 32-33 0.5722962466275305 1103 34-35 0.49561594983598667 1103 36-37 0.4454997674560275 1103 38-39 0.6041242294270859 1103 40-41 0.5186581456330686 1103 42-43 0.604546199972603 1103 44-45 0.6464832030284597 1103 46-47 0.48322355566720177 1103 48-49 0.5407837537855613 1103 50-51 0.4272018504086432 1103 52-53 0.4221214109616085 1103 54-55 0.4299293313566963 1103 56-57 0.43434874532316314 1103 58-59 0.45199997428763794 1103 60-61 0.5535852923829481 1103 62-63 0.5233248888352904 1103 64-65 0.3237176011116958 1103 66-67 0.40781976933939745 1103 68-69 0.4497089589534653 1103 70-71 0.41460768286501803 1103 72-73 0.3243085346003163 1103 74-75 0.26422885607301083 1103 76-77 0.2877916649704062 1103 78-79 0.19858674682067345 1103 80-81 0.20017187716300455 1103 82-83 0.2447993432221942 1103 84-85 0.19118987010290667 1103 86-87 0.5245189358759248 1103 88-89 0.46291293378862974 1103 90-91 0.3723315550030577 1103 92-93 0.2129398350839793 1103 94-95 0.23075141413457345 1103 96-97 0.21226279098577905 1103 98-99 0.02413118321624097 1103 100-101 -0.04095893928643335 1104 1 0.26018896842334627 1104 2 0.3198629775917432 1104 3 0.327261877243636 1104 4 0.3285046691012994 1104 5 0.33686833650317993 1104 6 0.2974836089222208 1104 7 0.33183810728971963 1104 8 0.3846003551374082 1104 9 0.45815318433871965 1104 10-11 0.5351253190945329 1104 12-13 0.5248854939937644 1104 14-15 0.5903574726763594 1104 16-17 0.5670751613311396 1104 18-19 0.4799157138911241 1104 20-21 0.5206246115184641 1104 22-23 0.5579553414689471 1104 24-25 0.5842080085912258 1104 26-27 0.5826203893102289 1104 28-29 0.6028643367613142 1104 30-31 0.7491832650146577 1104 32-33 0.7490070665451753 1104 34-35 0.750467613048734 1104 36-37 0.6378901725736981 1104 38-39 0.7414226152907446 1104 40-41 0.7588101561700711 1104 42-43 0.5817902255088114 1104 44-45 0.7133168615433902 1104 46-47 0.6460498485997519 1104 48-49 0.6443741228438356 1104 50-51 0.5680789844211276 1104 52-53 0.6934570151146104 1104 54-55 0.7417115507270253 1104 56-57 0.6187339526766209 1104 58-59 0.7311420639718094 1104 60-61 0.7780954125463495 1104 62-63 0.7139643792384689 1104 64-65 0.7065786344472187 1104 66-67 0.783469456625248 1104 68-69 0.762246072069594 1104 70-71 0.65847712128258 1104 72-73 0.623814672541311 1104 74-75 0.574944584428799 1104 76-77 0.75772648416687 1104 78-79 0.7115108202658824 1104 80-81 0.7164489951668251 1104 82-83 0.9032513077808098 1104 84-85 0.7739399862455194 1104 86-87 0.8114165200297911 1104 88-89 0.8949343364722893 1104 90-91 0.7941455709899188 1104 92-93 0.7777488758856066 1104 94-95 0.8152981840403015 1104 96-97 0.859231927499021 1104 98-99 0.9667170429893588 1104 100-101 1.0390514876211014 1105 1 0.22457444892848955 1105 2 0.2845993021695783 1105 3 0.2294308784814305 1105 4 0.2144379730042374 1105 5 0.26036581514549795 1105 6 0.27871286433748566 1105 7 0.2606882070410421 1105 8 0.30859509104217153 1105 9 0.2830445739133154 1105 10-11 0.3162750837513002 1105 12-13 0.29423954455442214 1105 14-15 0.4645107077038162 1105 16-17 0.3467074047749392 1105 18-19 0.4113161149824265 1105 20-21 0.37718336179909784 1105 22-23 0.3260534719433039 1105 24-25 0.3779729057747474 1105 26-27 0.26222693547914133 1105 28-29 0.39002023643060113 1105 30-31 0.379144266694702 1105 32-33 0.441686433132773 1105 34-35 0.5622402895574652 1105 36-37 0.5581387907747768 1105 38-39 0.6638019821651682 1105 40-41 0.7210319678501165 1105 42-43 0.4973099453967862 1105 44-45 0.6901302534350435 1105 46-47 0.6033745758448887 1105 48-49 0.6136653578436508 1105 50-51 0.6062231405270495 1105 52-53 0.6279390031632275 1105 54-55 0.6804366529880994 1105 56-57 0.6156566029665669 1105 58-59 0.6703963658207854 1105 60-61 0.7958291397568047 1105 62-63 0.6970689099118346 1105 64-65 0.8688082948129505 1105 66-67 0.8257666365481526 1105 68-69 0.8536893228126701 1105 70-71 0.6377791462695583 1105 72-73 0.5664970377961929 1105 74-75 0.5912293034549805 1105 76-77 0.6178595154779138 1105 78-79 0.7378109576569969 1105 80-81 0.811338692450061 1105 82-83 0.9345751907055408 1105 84-85 0.8571964841619639 1105 86-87 0.9145884711739392 1105 88-89 0.9303480756356919 1105 90-91 1.0086132540917987 1105 92-93 0.9426438951513916 1105 94-95 0.8834709291793956 1105 96-97 0.9053382623270814 1105 98-99 0.8026026550447298 1105 100-101 1.0483543313746324 1106 1 0.14468986533599448 1106 2 0.03984237534370294 1106 3 0.08925191246185449 1106 4 0.08903464984648224 1106 5 0.07273090521162828 1106 6 0.11344423116565139 1106 7 0.0796009685233443 1106 8 0.16157484866975835 1106 9 0.08429103940092375 1106 10-11 0.09145004467217888 1106 12-13 0.16280677036628788 1106 14-15 0.22746142197199504 1106 16-17 0.15906360740940073 1106 18-19 0.26467995032223257 1106 20-21 0.22019461801902196 1106 22-23 0.31229860495555073 1106 24-25 0.29799580337925846 1106 26-27 0.1695034018670043 1106 28-29 0.12795487659575855 1106 30-31 0.1843203836210776 1106 32-33 0.23134164170481597 1106 34-35 0.21309325281899305 1106 36-37 0.2806137115935101 1106 38-39 0.2582233988550229 1106 40-41 0.35967091459611567 1106 42-43 0.2651828901622295 1106 44-45 0.36125679102465824 1106 46-47 0.3612013980369184 1106 48-49 0.3239900086890728 1106 50-51 0.04838252043752789 1106 52-53 0.19857183433322234 1106 54-55 0.248040596296768 1106 56-57 0.20947795817330217 1106 58-59 0.25898762733991276 1106 60-61 0.22556970859334768 1106 62-63 0.2989049382043021 1106 64-65 0.11710504091321638 1106 66-67 0.193396078473711 1106 68-69 0.23524907857837718 1106 70-71 0.3067648358421806 1106 72-73 0.16514426839576046 1106 74-75 0.13985992286932714 1106 76-77 0.1368891051266523 1106 78-79 0.33694680518294007 1106 80-81 0.3362399332633963 1106 82-83 0.3769555415787842 1106 84-85 0.3176302777832696 1106 86-87 0.5009515448800315 1106 88-89 0.5145146749539542 1106 90-91 0.6159411397065924 1106 92-93 0.5003655259580171 1106 94-95 0.48967196518538714 1106 96-97 0.6483776130611965 1106 98-99 0.874890210901281 1106 100-101 1.1850750634637066 1107 1 0.050420575433818726 1107 2 0.17974432763475434 1107 3 0.14979772946468728 1107 4 0.13875230152095241 1107 5 0.12743446187447205 1107 6 0.07188485785844989 1107 7 -0.00722201286785662 1107 8 0.05713073780560052 1107 9 0.056747141231532794 1107 10-11 0.036527669301754884 1107 12-13 0.21523843370710694 1107 14-15 0.2072435940476396 1107 16-17 0.1558127555349813 1107 18-19 0.1317185216626129 1107 20-21 0.20724435932004326 1107 22-23 0.12455831512905036 1107 24-25 0.12477223040583141 1107 26-27 0.2199338946895537 1107 28-29 0.27333532490774104 1107 30-31 0.22068822707931446 1107 32-33 0.3094446104697539 1107 34-35 0.18792847909595878 1107 36-37 0.2193428553576524 1107 38-39 0.3763734895689481 1107 40-41 0.2172793082726301 1107 42-43 0.36999543297999793 1107 44-45 0.08484582111260153 1107 46-47 0.18742523936821698 1107 48-49 0.4106477558173012 1107 50-51 0.30663010621246656 1107 52-53 0.4018841620663878 1107 54-55 0.4282848517455804 1107 56-57 0.4372368662604771 1107 58-59 0.4713836075481552 1107 60-61 0.4071861241288275 1107 62-63 0.3882580264606901 1107 64-65 0.31689691420051247 1107 66-67 0.2924960589567718 1107 68-69 0.28278233951980525 1107 70-71 0.2214395427894189 1107 72-73 0.41857883836597054 1107 74-75 0.4639564430793115 1107 76-77 0.46253167036897835 1107 78-79 0.3853448854533106 1107 80-81 0.4727590952101508 1107 82-83 0.38464550061313574 1107 84-85 0.4683902682637857 1107 86-87 0.5003468683832004 1107 88-89 0.47149234050935007 1107 90-91 0.6964936208468586 1107 92-93 0.4820747465153232 1107 94-95 0.5227460145313714 1107 96-97 0.5046202568691491 1107 98-99 0.5069821525974199 1107 100-101 0.5497511893718432 1108 1 0.2666583014968076 1108 2 0.2294284603072292 1108 3 0.23832092436934005 1108 4 0.23734538744012212 1108 5 0.2015293049384752 1108 6 0.24355178497834373 1108 7 0.34038037331251303 1108 8 0.330692894116126 1108 9 0.40080156170293435 1108 10-11 0.4175362400416702 1108 12-13 0.3939735442017067 1108 14-15 0.49506900134161924 1108 16-17 0.5378958061851264 1108 18-19 0.49833916441576065 1108 20-21 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8166.0 11 10229.0 12 13634.0 13 18842.0 14 17076.0 15 17516.0 16 21933.0 17 28500.0 18 25130.0 19 18465.0 20 18476.0 21 19875.0 22 21485.0 23 24097.0 24 27281.0 25 30926.0 26 36072.0 27 41827.0 28 49068.0 29 57768.0 30 69521.0 31 84263.0 32 104529.0 33 133364.0 34 178445.0 35 251841.0 36 383681.0 37 580822.0 38 521427.0 39 79901.0 40 20.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.403469366728814 19.278606667161842 14.56532967678049 22.75259428932885 2 15.064976840816986 20.624717425185114 40.80960843155441 23.500697302443495 3 19.42650067671199 25.066245373752317 30.066776429590362 25.440477519945333 4 12.630307368797816 16.869405914860785 38.00755537689623 32.49273133944517 5 13.477959094166488 37.57816123913018 34.44337720769421 14.500502459009116 6 30.8567688979484 36.436105477365885 17.947183482044863 14.759942142640853 7 27.628795730728946 33.27126886002106 21.34055165689612 17.759383752353873 8 25.451091177549994 35.09097271416013 20.240443128184555 19.21749298010532 9 26.1350601004542 17.417216779511495 21.09104652083783 35.356676599196476 10-11 24.6168418382579 26.721597937713188 27.610363394067715 21.051196829961196 12-13 25.922257278885276 24.285988858490263 27.85435469984221 21.93739916278225 14-15 22.88324582316089 25.54994344170416 26.74559622787659 24.821214507258357 16-17 21.659024718808066 28.675688159367642 27.40840842993732 22.25687869188697 18-19 21.532329486056323 27.640544650423116 29.54431856901234 21.282807294508224 20-21 22.6765764987574 26.6060998797177 29.320731062366185 21.396592559158716 22-23 22.133752524331584 26.55369749270787 29.245640598013047 22.066909384947504 24-25 22.077432887137967 26.66923703973617 29.4642445510766 21.78908552204926 26-27 21.980238254191303 26.623360657836997 29.64587989625681 21.750521191714885 28-29 21.66234215917591 26.994573436010576 29.586597344785982 21.75648706002753 30-31 21.913412785700896 26.779269904607304 29.381577945463256 21.92573936422854 32-33 21.78040413369377 26.833910492401902 29.32186014512485 22.063825228779482 34-35 22.171566559504242 26.822136328747053 29.21698979404106 21.789307317707642 36-37 22.219502004760265 26.561671987377732 29.45518901768204 21.763636990179958 38-39 22.003461886596217 26.90534453381681 29.387300234077557 21.70389334550941 40-41 22.11088774526949 26.882877794398357 29.256140337648954 21.750094122683198 42-43 21.841725212836074 26.947425439878366 28.966910450716227 22.243938896569336 44-45 21.990666095448027 26.97304009176528 28.884959617825245 22.151334194961446 46-47 22.301909892724208 27.14551153010327 28.46169234806939 22.09088622910313 48-49 22.18387176472666 27.18132824412037 28.43002797958394 22.204772011569034 50-51 22.001105839087554 27.403738421907615 28.502119524917813 22.093036214087018 52-53 22.239839469690374 27.544238760032286 28.10787447528301 22.108047294994332 54-55 21.972990389417095 27.323568976988327 28.257541198681974 22.445899434912604 56-57 22.137110071018647 27.513881166655587 28.030170070429055 22.318838691896715 58-59 22.37136787354688 27.214757228180336 28.119861693827342 22.29401320444544 60-61 22.122133353523928 27.26345525404607 28.530443748318046 22.083967644111954 62-63 22.118030390401675 27.17202371606004 28.561334484349853 22.148611409188433 64-65 21.970605955964828 27.369281409959434 28.397959789024576 22.262152845051162 66-67 21.981782175229746 27.32514243982221 28.067388221156524 22.62568716379152 68-69 21.957397520077365 27.33893219236473 28.303609509679955 22.40006077787795 70-71 22.218599928350606 27.128401095285632 28.08493248270974 22.568066493654023 72-73 22.087421758078655 27.106290355547202 28.166611567527085 22.63967631884706 74-75 21.890987120195106 27.689411391904756 27.869868924797874 22.549732563102264 76-77 22.08910494090122 27.195022293292265 28.012011583700602 22.703861182105914 78-79 22.115764652003662 27.236360258313624 28.075409918110644 22.572465171572066 80-81 22.148614677788874 27.393542167815603 27.867600350628564 22.590242803766962 82-83 22.153353308327738 27.253353702593603 28.085700371312733 22.50759261776593 84-85 22.151319692734244 27.228462692403227 27.89205894145847 22.728158673404057 86-87 22.036521943645138 27.180607844233016 28.075708481342527 22.70716173077932 88-89 22.21783540865997 27.060649002303734 27.90129429476896 22.82022129426734 90-91 22.110393367996465 26.998701204065274 28.016511425546415 22.874394002391842 92-93 22.263376489586907 27.16169071736455 27.898935285509946 22.675997507538597 94-95 22.209513644839426 27.00982340641013 27.89788431338186 22.882778635368584 96-97 22.01940887914126 26.798155647228693 28.106506897290206 23.075928576339834 98-99 22.108701403877042 26.949833511119238 27.750290170254367 23.19117491474936 100-101 22.50660234470025 26.185691962582858 28.26765275395562 23.04005293876127 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2257.0 1 1315.0 2 450.5 3 545.5 4 515.5 5 546.0 6 909.0 7 1733.0 8 2783.5 9 3381.0 10 3292.5 11 3179.5 12 3438.0 13 3801.0 14 4102.5 15 4426.5 16 4766.0 17 4964.0 18 5124.0 19 5485.0 20 5989.0 21 6775.0 22 7886.5 23 9327.0 24 11200.5 25 13803.0 26 17985.0 27 23087.0 28 27058.5 29 30703.5 30 35104.5 31 40774.5 32 46839.5 33 52513.0 34 59341.0 35 65759.0 36 73656.5 37 81314.0 38 85087.5 39 87595.5 40 89262.5 41 91474.5 42 93802.0 43 96496.5 44 100539.0 45 103367.0 46 105277.5 47 111183.0 48 127636.0 49 127078.0 50 109161.5 51 101050.0 52 97017.0 53 93874.0 54 90293.0 55 84513.5 56 77012.5 57 69067.5 58 63091.5 59 57077.5 60 48470.0 61 39449.0 62 31502.0 63 24164.0 64 17204.0 65 12190.0 66 8735.0 67 6518.0 68 4902.0 69 3540.5 70 2578.5 71 1742.0 72 1173.0 73 871.5 74 618.0 75 452.0 76 312.5 77 167.0 78 92.0 79 63.5 80 49.0 81 35.5 82 38.0 83 32.5 84 25.0 85 23.5 86 12.0 87 9.0 88 10.0 89 9.5 90 7.5 91 6.0 92 3.5 93 1.5 94 3.0 95 4.0 96 4.5 97 3.0 98 1.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3231338007986396 2 0.08720620788103928 3 0.0974075600549167 4 0.10245705878981257 5 0.1153878697393094 6 0.05837629956355954 7 0.06045751187996932 8 0.06171988656369328 9 0.06441522710461743 10-11 0.06170282744634567 12-13 0.05997985659423593 14-15 0.058188649272735704 16-17 0.059945738359540694 18-19 0.06775881410475117 20-21 0.06963531701298949 22-23 0.0848179314523723 24-25 0.09365455423844006 26-27 0.08867329197293468 28-29 0.08596089231466292 30-31 0.08028020623790509 32-33 0.07255242607943271 34-35 0.3209331746607965 36-37 0.4964373739331228 38-39 0.47043927909534816 40-41 0.45204955059461255 42-43 0.4819712424223401 44-45 0.4917290575451793 46-47 0.4861677852898549 48-49 0.5032610208721713 50-51 0.49971272446386605 52-53 0.49983213828529943 54-55 0.4897843181675505 56-57 0.46817041648811464 58-59 0.45222014176808883 60-61 0.4139736006747222 62-63 0.48250007506011633 64-65 0.5054616470100144 66-67 0.5153047577195918 68-69 0.5271096669241456 70-71 0.5253355187199931 72-73 0.512728831000101 74-75 0.5139059100970869 76-77 0.48787369702461697 78-79 0.46929631823305756 80-81 0.47550583694759163 82-83 0.4834724447489307 84-85 0.48534894765716907 86-87 0.48505894266225946 88-89 0.4918143531319175 90-91 0.5056834155355335 92-93 0.5114664563163771 94-95 0.518358339724816 96-97 0.509982313107134 98-99 0.5125752989439724 100-101 0.4915243481370079 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2930984.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.270260199561726 #Duplication Level Percentage of deduplicated Percentage of total 1 75.55995010851166 41.762181031633425 2 13.739661846451837 15.187893706147676 3 4.603405172512472 7.632942050663181 4 2.0608371513214463 4.556120223298396 5 1.1419569218482037 3.1558128103620406 6 0.67514136934032 2.238914349295674 7 0.4334014447839574 1.676794743685272 8 0.2925670038920065 1.293620354473391 9 0.21821984095251873 1.085496065113742 >10 1.148556693585274 11.425613284361482 >50 0.08283416481758732 3.117977072834181 >100 0.040187136902115735 3.9062195235593706 >500 0.0018572519325264485 0.6774268300585882 >1k 0.0011762595571089705 1.2243160624159 >5k 1.857251932277322E-4 0.6888552854463793 >10k+ 6.19083977425774E-5 0.36981660665132465 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10808 0.3687498805861786 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8455 0.2884696743482735 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6457 0.22030144142717942 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5220 0.17809718510916472 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3648 0.12446332016824382 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3454 0.11784438263736684 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.705911734762114E-5 3.411823469524228E-5 12-13 0.0 0.0 0.0 3.411823469524228E-5 1.0235470408572684E-4 14-15 0.0 0.0 0.0 3.411823469524228E-5 2.2176852551907483E-4 16-17 0.0 0.0 0.0 3.411823469524228E-5 2.3882764286669596E-4 18-19 0.0 0.0 0.0 3.411823469524228E-5 2.7294587756193823E-4 20-21 0.0 0.0 0.0 8.529558673810571E-5 2.7294587756193823E-4 22-23 0.0 0.0 0.0 1.8765029082383255E-4 3.2412322960480164E-4 24-25 0.0 0.0 0.0 5.288326377762553E-4 3.753005816476651E-4 26-27 0.0 0.0 0.0 0.0011600199796382375 3.753005816476651E-4 28-29 0.0 0.0 0.0 0.0035653555256528182 3.753005816476651E-4 30-31 0.0 1.705911734762114E-5 0.0 0.00943369189323449 3.753005816476651E-4 32-33 0.0 3.411823469524228E-5 0.0 0.02260333048559801 3.753005816476651E-4 34-35 0.0 3.411823469524228E-5 0.0 0.04324486247621959 4.094188163429074E-4 36-37 0.0 3.411823469524228E-5 0.0 0.07761898393167618 4.094188163429074E-4 38-39 0.0 3.411823469524228E-5 0.0 0.12819926686737287 4.094188163429074E-4 40-41 0.0 3.411823469524228E-5 0.0 0.18423846735430832 4.094188163429074E-4 42-43 0.0 3.411823469524228E-5 0.0 0.2433824271985108 4.094188163429074E-4 44-45 0.0 3.411823469524228E-5 0.0 0.31535484328812435 4.094188163429074E-4 46-47 0.0 3.411823469524228E-5 0.0 0.403311652332459 4.605961683857708E-4 48-49 0.0 3.411823469524228E-5 0.0 0.49889388683118024 4.776552857333919E-4 50-51 0.0 3.411823469524228E-5 0.0 0.6014021229730356 4.776552857333919E-4 52-53 0.0 3.411823469524228E-5 0.0 0.7039615364669339 4.776552857333919E-4 54-55 0.0 3.411823469524228E-5 0.0 0.8236483037778439 5.117735204286342E-4 56-57 0.0 3.411823469524228E-5 0.0 0.9654095689365756 5.117735204286342E-4 58-59 0.0 3.411823469524228E-5 0.0 1.1254411487746094 5.117735204286342E-4 60-61 0.0 3.411823469524228E-5 0.0 1.303538333883774 5.117735204286342E-4 62-63 0.0 3.411823469524228E-5 0.0 1.4861050077380156 5.458917551238765E-4 64-65 0.0 3.411823469524228E-5 0.0 1.6765871120415534 5.458917551238765E-4 66-67 0.0 3.411823469524228E-5 0.0 1.8974515043412041 5.458917551238765E-4 68-69 0.0 3.411823469524228E-5 0.0 2.1534235601422593 5.458917551238765E-4 70-71 0.0 3.411823469524228E-5 0.0 2.4366049081127703 5.458917551238765E-4 72-73 0.0 3.411823469524228E-5 0.0 2.7258593018590345 5.800099898191187E-4 74-75 0.0 3.411823469524228E-5 0.0 3.030262191809986 5.800099898191187E-4 76-77 0.0 3.411823469524228E-5 0.0 3.356398397261807 5.800099898191187E-4 78-79 0.0 3.411823469524228E-5 0.0 3.736902009700496 6.14128224514361E-4 80-81 0.0 3.411823469524228E-5 0.0 4.155652163232553 6.311873418619821E-4 82-83 0.0 3.411823469524228E-5 0.0 4.578001790524957 7.676602806429513E-4 84-85 0.0 3.411823469524228E-5 0.0 5.016864643409859 8.188376326858148E-4 86-87 0.0 3.411823469524228E-5 0.0 5.48319267522443 8.52955867381057E-4 88-89 0.0 3.411823469524228E-5 0.0 5.98846667194362 8.52955867381057E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 12210 0.0 23.519682 1 TACATGG 12025 0.0 22.963978 2 GAGTACT 9845 0.0 21.714134 12-13 ACATGGG 12435 0.0 21.292646 3 GTATCAA 23210 0.0 21.146435 1 ACCGGTC 115 0.003795248 20.58533 8 AGTACTT 10315 0.0 20.56408 12-13 GTACTTT 10745 0.0 19.940105 14-15 CATGGGA 7300 0.0 19.01115 4 GGTATCA 18240 0.0 18.632877 1 ATCAACG 26535 0.0 18.225115 3 TATCAAC 26815 0.0 18.17612 2 TCAACGC 26655 0.0 18.143066 4 CAACGCA 26730 0.0 18.07444 5 AACGCAG 27180 0.0 17.872124 6 GTATAGG 830 0.0 17.728636 1 AGAGTAC 20255 0.0 16.79472 10-11 TACTTTT 12720 0.0 16.583488 14-15 ACGCAGA 28915 0.0 16.554407 7 ATGGGAT 3170 0.0 16.436035 5 >>END_MODULE