FastQCFastQC Report
Thu 26 May 2016
SRR938931_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938931_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2930984
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108870.3714452211271027No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91920.31361481331866703No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT72450.24718661036703032No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54590.1862514432013276No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA33970.11589964325973802No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT32900.1122489921473471No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACT107500.021.89561812-13
GTACATG119850.021.7793451
TACATGG117300.021.2377382
GTATCAA249100.020.3647061
GTACTTT115400.020.21164914-15
AGTACTT111850.019.96216612-13
GGTATCA196700.019.493681
ACATGGG121550.019.4801623
ATCAACG277850.017.9831163
TCAACGC279350.017.903544
CAACGCA279400.017.8833525
TATCAAC283800.017.6562522
AACGCAG284450.017.5658596
GTATAGG7600.017.548771
ACTTTTT137450.017.05469716-17
AGAGTAC210400.016.69052710-11
CATGGGA73650.016.4935534
TACTTTT135850.016.19101714-15
ACGCAGA307800.016.0945517
CGCAGAG307250.015.8917038
Produced by FastQC (version 0.11.5)