##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938931_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2930984 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.19094884175417 33.0 31.0 34.0 30.0 34.0 2 32.42799687749916 34.0 31.0 34.0 31.0 34.0 3 32.51534058186602 34.0 31.0 34.0 30.0 34.0 4 36.06011940017414 37.0 35.0 37.0 35.0 37.0 5 35.98808147707391 37.0 35.0 37.0 35.0 37.0 6 36.02996297489171 37.0 35.0 37.0 35.0 37.0 7 35.96991965838094 37.0 35.0 37.0 35.0 37.0 8 35.939369508670126 37.0 35.0 37.0 35.0 37.0 9 37.62092525923035 39.0 37.0 39.0 35.0 39.0 10-11 37.53288963706387 39.0 37.0 39.0 35.0 39.0 12-13 37.44162660048639 39.0 37.0 39.0 34.0 39.0 14-15 38.75391472624893 40.0 38.0 41.0 34.5 41.0 16-17 38.845125561927325 40.0 38.0 41.0 35.0 41.0 18-19 38.89647384632601 40.0 38.0 41.0 35.0 41.0 20-21 38.87009021543618 40.0 38.0 41.0 34.5 41.0 22-23 38.792752195167225 40.0 38.0 41.0 34.0 41.0 24-25 38.71983521575007 40.0 38.0 41.0 34.0 41.0 26-27 38.554710295245556 40.0 38.0 41.0 34.0 41.0 28-29 38.39757279466555 40.0 38.0 41.0 34.0 41.0 30-31 38.20279639875209 40.0 38.0 41.0 33.5 41.0 32-33 38.10184412470351 40.0 38.0 41.0 33.0 41.0 34-35 38.006392051270154 40.0 37.0 41.0 33.0 41.0 36-37 37.87604742980514 40.0 37.0 41.0 33.0 41.0 38-39 37.729833223245166 40.0 37.0 41.0 33.0 41.0 40-41 37.55384096262552 40.0 37.0 41.0 32.5 41.0 42-43 37.36614375922898 40.0 37.0 41.0 32.0 41.0 44-45 37.10644172741987 40.0 36.5 41.0 31.0 41.0 46-47 36.78550292325035 40.0 36.0 41.0 30.5 41.0 48-49 36.43966787263253 39.0 36.0 41.0 30.0 41.0 50-51 36.17187794269775 39.0 35.0 41.0 29.5 41.0 52-53 35.90730314460946 39.0 35.0 40.0 28.5 41.0 54-55 35.605225241761815 38.5 35.0 40.0 28.0 41.0 56-57 35.460917732747774 38.0 35.0 40.0 28.0 41.0 58-59 35.183625533268014 38.0 34.5 40.0 27.5 41.0 60-61 34.81147406468271 38.0 34.0 40.0 26.5 41.0 62-63 34.387918528385 37.0 34.0 40.0 26.0 41.0 64-65 33.97153123319677 37.0 33.0 39.5 25.5 41.0 66-67 33.5064901411949 36.0 33.0 39.0 24.5 40.5 68-69 33.00623425443469 35.5 32.0 39.0 23.0 40.0 70-71 32.47781103547477 35.0 31.5 38.0 22.0 40.0 72-73 31.943655100130194 35.0 31.0 37.5 20.5 39.5 74-75 31.41064400215081 35.0 31.0 37.0 19.0 39.0 76-77 29.504266655839814 32.5 28.5 35.0 15.5 37.0 78-79 30.462963120917752 34.0 30.0 36.0 17.5 38.0 80-81 30.43111835479143 34.0 30.0 35.5 15.5 37.0 82-83 29.999611734489168 34.0 30.0 35.0 9.5 37.0 84-85 29.525014295540338 34.0 29.0 35.0 6.0 36.0 86-87 28.81062929719166 34.0 29.0 35.0 2.0 36.0 88-89 28.09909538912529 33.0 27.5 35.0 2.0 35.0 90-91 27.485403195650335 33.0 26.0 35.0 2.0 35.0 92-93 27.051613212491095 32.5 26.0 34.0 2.0 35.0 94-95 26.46466340314379 32.0 25.0 34.0 2.0 35.0 96-97 25.57079891940727 32.0 23.5 34.0 2.0 35.0 98-99 24.29559014992917 31.5 10.0 34.0 2.0 35.0 100-101 18.624378024581503 24.0 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.13580066119429546 1101 2 0.18371647716376316 1101 3 0.11529727961431036 1101 4 0.0916285991335215 1101 5 0.147997752221265 1101 6 0.13263753055100125 1101 7 0.1731180748260357 1101 8 0.13569540580341766 1101 9 0.11189692981957933 1101 10-11 0.25408477843706834 1101 12-13 0.22552150319754816 1101 14-15 0.21821625056765015 1101 16-17 0.1963285964967909 1101 18-19 0.23957525425512216 1101 20-21 0.2459510078246936 1101 22-23 0.2631613589679489 1101 24-25 0.15752078847434348 1101 26-27 0.07023732320839571 1101 28-29 0.14287236300526018 1101 30-31 0.17000005785018146 1101 32-33 0.1745056171335051 1101 34-35 0.23145808112333555 1101 36-37 0.33527007093826455 1101 38-39 0.1352054834434 1101 40-41 0.10603665866867829 1101 42-43 0.04538615711710037 1101 44-45 0.06956014388916998 1101 46-47 0.07631062818285272 1101 48-49 0.007244232945311069 1101 50-51 -0.13175143712727788 1101 52-53 -0.09021965049896608 1101 54-55 -0.012186940752414444 1101 56-57 -0.07567508193914563 1101 58-59 0.11217145321051447 1101 60-61 0.007906973262187478 1101 62-63 -0.011842749080884118 1101 64-65 -0.011215429001573796 1101 66-67 -0.20336992954322142 1101 68-69 -0.1286499918043731 1101 70-71 0.03865706845762418 1101 72-73 0.08434713628182422 1101 74-75 -0.10309164147389183 1101 76-77 -0.30395478907816553 1101 78-79 0.006708168839001161 1101 80-81 0.036953937414867966 1101 82-83 -0.05264033913654487 1101 84-85 0.11445470491745269 1101 86-87 0.27079449515620624 1101 88-89 0.2919507790604996 1101 90-91 0.28806718349083127 1101 92-93 0.14568117300391847 1101 94-95 0.14313409828863755 1101 96-97 0.32449093687746 1101 98-99 0.34163706016579454 1101 100-101 0.6487196005736813 1102 1 0.15391213335379206 1102 2 0.16354932399969613 1102 3 0.15729347942705374 1102 4 0.1050338973656082 1102 5 0.05010162400625262 1102 6 0.1156358232204937 1102 7 0.07977391004110501 1102 8 0.13372233756527407 1102 9 0.1427610593563955 1102 10-11 0.2484003316672272 1102 12-13 0.2597693139592323 1102 14-15 0.270892215760135 1102 16-17 0.2525693025605662 1102 18-19 0.23918697425290958 1102 20-21 0.2684781323496779 1102 22-23 0.24432909042138107 1102 24-25 0.28299512344157307 1102 26-27 0.35631707206558616 1102 28-29 0.3573282202504018 1102 30-31 0.2676572974822804 1102 32-33 0.31947863029647294 1102 34-35 0.25324857514767984 1102 36-37 0.30231859216974044 1102 38-39 0.30818284754539604 1102 40-41 0.35785551640950075 1102 42-43 0.36884887122185006 1102 44-45 0.3692620494903096 1102 46-47 0.21311943470374928 1102 48-49 0.2731354870071101 1102 50-51 0.06186810354573424 1102 52-53 0.1427373357873094 1102 54-55 0.21603220337932783 1102 56-57 0.29157033305231295 1102 58-59 0.25734686765122916 1102 60-61 0.18454132915798027 1102 62-63 0.18927321170558287 1102 64-65 0.2619388641085223 1102 66-67 0.05665444122288221 1102 68-69 0.190815641284793 1102 70-71 0.3107429031219482 1102 72-73 0.3152326074499747 1102 74-75 0.29810486675985004 1102 76-77 0.14140030812952276 1102 78-79 0.27824807080518354 1102 80-81 0.31682740219429206 1102 82-83 0.35158530386615183 1102 84-85 0.6314068445881027 1102 86-87 0.7369253262406374 1102 88-89 0.6421235774302758 1102 90-91 0.652303428465224 1102 92-93 0.7191308067539879 1102 94-95 0.7666780482803759 1102 96-97 0.9368828518413821 1102 98-99 1.233530682460227 1102 100-101 1.1279782886829501 1103 1 0.12934939800373257 1103 2 0.14875043011070943 1103 3 0.15001208434590296 1103 4 0.12095849764734368 1103 5 0.11898197436609337 1103 6 0.1879605747840145 1103 7 0.14407058677495144 1103 8 0.2344191381747862 1103 9 0.16189930130858698 1103 10-11 0.16190147724305604 1103 12-13 0.10120771048194399 1103 14-15 0.22792085789649263 1103 16-17 0.2154383259708723 1103 18-19 0.25384294522434914 1103 20-21 0.2939147118466039 1103 22-23 0.11586201065312451 1103 24-25 0.20459477757633948 1103 26-27 0.16736036825448508 1103 28-29 0.19833640919916462 1103 30-31 0.2541734177622672 1103 32-33 0.29958384239243685 1103 34-35 0.25388823399629246 1103 36-37 0.34220518564379887 1103 38-39 0.352973492192163 1103 40-41 0.34278694629642104 1103 42-43 0.3442912745193567 1103 44-45 0.36997495119552326 1103 46-47 0.4856640518676514 1103 48-49 0.3462496033515663 1103 50-51 0.22920822994277046 1103 52-53 0.494885975621564 1103 54-55 0.6803010629010373 1103 56-57 0.5544836858622588 1103 58-59 0.43235248590590203 1103 60-61 0.4137896889016872 1103 62-63 0.3948853685124476 1103 64-65 0.39381951319507635 1103 66-67 0.4308356148625805 1103 68-69 0.35260006748284667 1103 70-71 0.4248062852564374 1103 72-73 0.3571889796470096 1103 74-75 0.44743245759743644 1103 76-77 0.16572728232939937 1103 78-79 0.4609504238946762 1103 80-81 0.5859710568552501 1103 82-83 0.5501112656839453 1103 84-85 0.5646430689017201 1103 86-87 0.6741670235564818 1103 88-89 0.5027046008844316 1103 90-91 0.5081615552020864 1103 92-93 0.629271756430871 1103 94-95 0.923630227341004 1103 96-97 0.6104104580263972 1103 98-99 0.9981608231632464 1103 100-101 1.2672301564100472 1104 1 0.117239054144477 1104 2 0.10918708378872566 1104 3 0.13344125250950611 1104 4 0.11098459283828532 1104 5 0.08172698741368833 1104 6 0.1596391952092091 1104 7 0.12844564910105305 1104 8 0.08583982759520836 1104 9 0.1132096851629214 1104 10-11 0.21369357039754533 1104 12-13 0.1668590012937372 1104 14-15 0.287442384899947 1104 16-17 0.2713261248935055 1104 18-19 0.2284976619440755 1104 20-21 0.23053488335208527 1104 22-23 0.2586774698133425 1104 24-25 0.2549972147230406 1104 26-27 0.21682103307156098 1104 28-29 0.3170629463303598 1104 30-31 0.15997697173951764 1104 32-33 0.3096801390452768 1104 34-35 0.45242449136672747 1104 36-37 0.3028795095235992 1104 38-39 0.3638184998414786 1104 40-41 0.5003828327604012 1104 42-43 0.37292943689457303 1104 44-45 0.3317317880531121 1104 46-47 0.3021171056538563 1104 48-49 0.2831118992975803 1104 50-51 0.24300333974706234 1104 52-53 0.33174648987206723 1104 54-55 0.5426933401285936 1104 56-57 0.30636304220747945 1104 58-59 0.390198900030974 1104 60-61 0.37028008649367905 1104 62-63 0.46936024035667856 1104 64-65 0.48485285137710576 1104 66-67 0.3428888646884616 1104 68-69 0.38267355843330364 1104 70-71 0.4305604969053789 1104 72-73 0.3938306112758774 1104 74-75 0.4846377166306972 1104 76-77 0.38007023690706987 1104 78-79 0.6417938466327797 1104 80-81 0.7889070384085848 1104 82-83 0.6452852509086355 1104 84-85 0.6229455305932703 1104 86-87 0.6536813812299904 1104 88-89 0.677008935217728 1104 90-91 0.7753111721508503 1104 92-93 0.8968646033755157 1104 94-95 1.0381970373322211 1104 96-97 0.9483994902280166 1104 98-99 1.0499487280036917 1104 100-101 1.0277264702948017 1105 1 0.067548399269441 1105 2 0.03559757213604087 1105 3 -0.01673774185221788 1105 4 0.011325450045134744 1105 5 0.07224728747858933 1105 6 0.046883438224334384 1105 7 0.04967404924425978 1105 8 0.1309934988635746 1105 9 0.07238230853004524 1105 10-11 0.09791144104651295 1105 12-13 0.20088416193756586 1105 14-15 0.15992197245022055 1105 16-17 -0.047808736126079054 1105 18-19 0.08416437389495712 1105 20-21 0.11837964208583429 1105 22-23 0.08467764921187637 1105 24-25 -0.015293658611426508 1105 26-27 0.032252931121043105 1105 28-29 0.0893950646384738 1105 30-31 0.059883217754880036 1105 32-33 0.1658049300186093 1105 34-35 0.1944973188257606 1105 36-37 0.1569993654728421 1105 38-39 0.08394134350636051 1105 40-41 0.12980218386444875 1105 42-43 0.25198842641670893 1105 44-45 0.1839884937902383 1105 46-47 0.23587263395774727 1105 48-49 0.2341625671268588 1105 50-51 0.31778853738661894 1105 52-53 0.29132810538190057 1105 54-55 0.31587056110456047 1105 56-57 0.27644247038509917 1105 58-59 0.2784036292161147 1105 60-61 0.37482613129063935 1105 62-63 0.27095785206159917 1105 64-65 0.21730953953943555 1105 66-67 0.2687525525746537 1105 68-69 0.3497229636932744 1105 70-71 0.47702302125651386 1105 72-73 0.4738526553917559 1105 74-75 0.30461637654975604 1105 76-77 0.13599886288903207 1105 78-79 0.36248532887923446 1105 80-81 0.5795769198204219 1105 82-83 0.4740100454803695 1105 84-85 0.5523321962238761 1105 86-87 0.6374377309079726 1105 88-89 0.8648888696225363 1105 90-91 0.741052897719598 1105 92-93 0.9933931375200338 1105 94-95 1.0310040819578639 1105 96-97 1.1033459092586568 1105 98-99 1.3968114831739769 1105 100-101 1.2833470158071414 1106 1 -0.0024226699026570486 1106 2 -0.06662579472486385 1106 3 -0.07045414608176515 1106 4 0.0019958207034704856 1106 5 -0.042481271176924906 1106 6 0.013246491470155775 1106 7 0.056607780461732204 1106 8 0.012263933562557838 1106 9 -0.040185005772507054 1106 10-11 -0.002569605578365497 1106 12-13 -0.020549970301281917 1106 14-15 -0.11332006799156602 1106 16-17 -0.0978340429279001 1106 18-19 -0.17875686479050756 1106 20-21 -0.023384995194426494 1106 22-23 -0.1681020069327559 1106 24-25 0.05884951625026247 1106 26-27 -0.07716484363997722 1106 28-29 -0.049571054681756266 1106 30-31 -0.04188995913236937 1106 32-33 -0.00798649436431731 1106 34-35 -0.12273514262708574 1106 36-37 -0.05176998483160844 1106 38-39 -0.032910160426105506 1106 40-41 0.16197059442849593 1106 42-43 0.014487863759164554 1106 44-45 0.07234994806561446 1106 46-47 0.06693982140659926 1106 48-49 0.1867495897886613 1106 50-51 0.14383522108305158 1106 52-53 0.11229667686600919 1106 54-55 0.12486923272125239 1106 56-57 0.11612087579315045 1106 58-59 0.07175441090089407 1106 60-61 0.03865640717697971 1106 62-63 0.25658738656990465 1106 64-65 0.18812557967839183 1106 66-67 0.08246431232192464 1106 68-69 0.11026102798764015 1106 70-71 0.18719939905138716 1106 72-73 0.04635063238213277 1106 74-75 0.18683610403936157 1106 76-77 0.055094974004695985 1106 78-79 0.2890938772841487 1106 80-81 0.22224686030148533 1106 82-83 0.15914445413752532 1106 84-85 0.1929612882787417 1106 86-87 0.4699487743410451 1106 88-89 0.4765022875727425 1106 90-91 0.7594422725968357 1106 92-93 0.6323813155735252 1106 94-95 0.575125512663007 1106 96-97 0.6398520258576639 1106 98-99 0.8602315177723057 1106 100-101 1.1645125916001824 1107 1 -0.04409232385543049 1107 2 -0.023552365240746553 1107 3 0.014635711484430658 1107 4 -0.027362480367173703 1107 5 -0.0556859470762987 1107 6 0.008940069601628409 1107 7 -0.02493990259916501 1107 8 -0.05250229636287429 1107 9 -0.1149796796405198 1107 10-11 -0.07036892666780403 1107 12-13 -0.03422999810295835 1107 14-15 -0.14011459299315732 1107 16-17 -0.02014748939971156 1107 18-19 -0.06892101367285619 1107 20-21 0.01554681103824862 1107 22-23 -0.013760260405966562 1107 24-25 -0.056286713953987544 1107 26-27 0.02664341092857825 1107 28-29 0.03274778384149357 1107 30-31 -0.022167958467207427 1107 32-33 0.008353525013220064 1107 34-35 0.0664915087446758 1107 36-37 0.04810830815344502 1107 38-39 0.01456235903108194 1107 40-41 0.037120207674526284 1107 42-43 0.05155684816154604 1107 44-45 -0.08612694925936637 1107 46-47 -0.06061082305058818 1107 48-49 -0.09695210081612515 1107 50-51 -0.1579256720557538 1107 52-53 -0.2326420763005146 1107 54-55 -0.2319229472607418 1107 56-57 -0.1690345029150606 1107 58-59 -0.10062824103247436 1107 60-61 -0.06257848711291558 1107 62-63 0.046771799209707865 1107 64-65 -0.06238555742210394 1107 66-67 -0.1687191508587702 1107 68-69 -0.15690690528833073 1107 70-71 0.023524748364707193 1107 72-73 0.12200284406918982 1107 74-75 0.07964524583189458 1107 76-77 -0.16677039199760202 1107 78-79 0.1661607680968551 1107 80-81 0.015471209969295074 1107 82-83 -0.025399252845559772 1107 84-85 0.13583292658610802 1107 86-87 0.16133685168387402 1107 88-89 0.31480714958004086 1107 90-91 0.40057567135173855 1107 92-93 0.47164988852703615 1107 94-95 0.6044732337620111 1107 96-97 0.696431847138296 1107 98-99 0.425708659556868 1107 100-101 0.4379753590618627 1108 1 0.05367071718884375 1108 2 0.06907463912969547 1108 3 0.0674510847075922 1108 4 0.07891529999621127 1108 5 0.08406530685147118 1108 6 0.06192811133014686 1108 7 0.1101002380985534 1108 8 0.13524991916071372 1108 9 0.14995144782427872 1108 10-11 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-0.5916113714278666 2316 54-55 -0.6366029929262922 2316 56-57 -0.6489310415438183 2316 58-59 -0.7560809847395689 2316 60-61 -0.6663091762448801 2316 62-63 -0.6768663534070782 2316 64-65 -0.7413517570760391 2316 66-67 -0.6602326384040325 2316 68-69 -0.6176077052546276 2316 70-71 -0.5349562928846687 2316 72-73 -0.48660804321988493 2316 74-75 -0.618326955763596 2316 76-77 -0.6868955041280529 2316 78-79 -0.7682465014088713 2316 80-81 -0.7600115873321975 2316 82-83 -0.669669233131664 2316 84-85 -0.9486565280312682 2316 86-87 -1.0501373777685323 2316 88-89 -1.278577695005545 2316 90-91 -1.4089127153565713 2316 92-93 -1.2656803212454157 2316 94-95 -1.1807987787696845 2316 96-97 -1.095653132676759 2316 98-99 -1.0993573606298312 2316 100-101 -0.6427693919456274 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 12.0 4 24.0 5 57.0 6 128.0 7 337.0 8 825.0 9 1566.0 10 2766.0 11 4277.0 12 6183.0 13 8594.0 14 10665.0 15 14335.0 16 18908.0 17 26685.0 18 26503.0 19 19096.0 20 17765.0 21 18768.0 22 20568.0 23 22834.0 24 26364.0 25 31129.0 26 36647.0 27 44293.0 28 53217.0 29 64580.0 30 78055.0 31 94022.0 32 117197.0 33 148997.0 34 199900.0 35 286874.0 36 444225.0 37 650832.0 38 413357.0 39 20398.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.33285300668838 19.922206949654072 15.090111767568942 22.654828276088605 2 15.445495437709656 21.219392531654897 39.947335092924426 23.38777693771102 3 19.403995381755752 25.207575339885853 30.350285091969116 25.03814418638928 4 13.077997013972102 17.469559711004905 37.40191007525118 32.05053319977182 5 13.43374784713939 37.82156436200266 34.38241218648754 14.362275604370408 6 30.895494482399084 36.898495522322875 17.554104696579714 14.65190529869832 7 27.610590845941157 33.39786228788694 21.08012189762892 17.91142496854299 8 25.565202675961384 35.40544745382438 19.910651166980102 19.118698703234134 9 25.99154413671313 17.28839188477317 20.91976619456128 35.80029778395242 10-11 24.641622062761176 26.938666331853057 27.37251380423776 21.04719780114801 12-13 25.860513056366052 24.3983249311494 27.816511451444292 21.924650561040252 14-15 22.74294863899495 25.725794867823126 26.627581620896095 24.903674872285826 16-17 21.556596112876967 28.862864341016504 27.3434717159324 22.23706783017413 18-19 21.438770375045845 27.74399303985875 29.52703451922995 21.290202065865454 20-21 22.558192061096204 26.677098885561985 29.394513924333943 21.370195129007868 22-23 22.09769824741452 26.664611611663524 29.224502760847553 22.013187380074406 24-25 22.02980469170481 26.801465652376177 29.494300799919753 21.67442885599926 26-27 21.98311590631609 26.702982121871376 29.65086633889273 21.6630356329198 28-29 21.647320490319974 27.12508836622786 29.58735018683145 21.640240956620712 30-31 21.95361694229651 26.848491837553535 29.380508388991544 21.81738283115841 32-33 21.77161321931474 26.926281412658682 29.332913451591686 21.96919191643489 34-35 22.15750070804855 26.879638571072718 29.280537366195887 21.682323354682843 36-37 22.231934975552697 26.593425719192602 29.512768820071102 21.661870485183595 38-39 21.942912401452723 26.900623806466616 29.488798764363988 21.667665027716676 40-41 22.0652574641144 26.950465682398345 29.27300045226459 21.71127640122267 42-43 21.808724069667544 27.00888211341891 29.01924844600547 22.163145370908076 44-45 21.963131156322397 27.041655720198026 28.89239934082356 22.102813782656018 46-47 22.292734154774603 27.235046295632877 28.456221984301315 22.015997565291208 48-49 22.179610971411414 27.296725568001527 28.415174230353234 22.10848923023382 50-51 22.004934896824015 27.494871139622028 28.43254930205533 22.06764466149863 52-53 22.24150660665497 27.62625111566627 28.031729275901878 22.10051300177688 54-55 21.99972773648713 27.426676501816456 28.14515534714622 22.4284404145502 56-57 22.150871516187053 27.577922636220464 28.01801374555439 22.253192102038085 58-59 22.35355771840509 27.3391281384571 28.01209911365173 22.295215029486087 60-61 22.136603049365604 27.408056295980384 28.428258876212816 22.0270817784412 62-63 22.114503762099773 27.265883369277393 28.463859583758538 22.15575328486429 64-65 21.946425448977937 27.438000315595986 28.288812975259837 22.326761260166244 66-67 21.92307777605855 27.446202022555905 27.958895156787406 22.671825044598144 68-69 21.89401875973142 27.478982178033373 28.11518488057701 22.511814181658192 70-71 22.170197565184637 27.243650762781897 27.960151239413953 22.626000432619513 72-73 22.03150887210575 27.186262361036427 28.028914521539523 22.753314245318297 74-75 21.802797285826195 27.79489754976486 27.668864790800633 22.733440373608317 76-77 21.92648286443668 27.28686046100644 27.93129348817347 22.85536318638341 78-79 21.98926262886466 27.3280084343125 27.941969746339417 22.74075919048342 80-81 21.943415162064422 27.49350515757616 27.770085051771943 22.792994628587472 82-83 22.050256842070787 27.35064401579811 27.909364227167394 22.68973491496371 84-85 21.907943355426074 27.30972392150333 27.762217935446802 23.02011478762379 86-87 21.742676126754983 27.202830923232856 27.987587732119586 23.06690521789257 88-89 21.938533088031818 27.089076697504133 27.75666487265517 23.21572534180888 90-91 21.813630080787732 27.013292564695863 27.84579686456662 23.327280489949786 92-93 21.813783357398062 27.244809251773468 27.752932121089707 23.188475269738763 94-95 21.758342164360137 27.072959862215512 27.71667518493978 23.45202278848457 96-97 21.52850373799379 26.928004383510796 27.91714318467791 23.626348693817505 98-99 21.541485726295335 27.093443703548026 27.543343805356834 23.82172676479981 100-101 21.787378225228505 26.104594222842948 28.435986051738084 23.672041500190467 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 680.0 1 491.0 2 344.0 3 417.0 4 389.5 5 390.5 6 659.0 7 1406.5 8 2389.5 9 2934.0 10 2810.5 11 2576.5 12 2739.5 13 3008.5 14 3312.5 15 3536.5 16 3753.0 17 4093.5 18 4430.5 19 4858.5 20 5457.5 21 6421.5 22 7694.0 23 9328.0 24 11188.5 25 13760.5 26 18158.0 27 23295.5 28 27228.0 29 30895.0 30 35344.0 31 41052.5 32 47262.0 33 53015.0 34 59420.5 35 65813.5 36 73699.0 37 81277.5 38 85238.0 39 87623.5 40 89358.5 41 91639.0 42 93726.0 43 96630.5 44 101111.0 45 104012.5 46 105379.5 47 111727.5 48 128891.5 49 127876.0 50 110318.5 51 102861.5 52 98591.5 53 95289.5 54 91495.5 55 85462.0 56 77694.0 57 69682.5 58 63622.0 59 57185.0 60 48513.0 61 39170.5 62 31010.0 63 23670.0 64 16739.5 65 11878.5 66 8485.0 67 6223.5 68 4535.5 69 3253.0 70 2362.5 71 1504.5 72 988.5 73 739.5 74 507.0 75 345.0 76 225.5 77 104.0 78 55.5 79 27.0 80 13.5 81 7.5 82 6.5 83 5.0 84 3.0 85 1.5 86 1.0 87 1.0 88 0.0 89 0.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.39041495961765743 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 8.52955867381057E-4 16-17 0.002592985836838413 18-19 3.0706411225718056E-4 20-21 0.0 22-23 0.0 24-25 8.52955867381057E-5 26-27 1.7059117347621143E-5 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.013442584469925458 36-37 0.02393394163871246 38-39 0.011856086556596692 40-41 0.006055986658405505 42-43 0.005117735204286342 44-45 0.0018423846735430831 46-47 0.0012964929184192066 48-49 0.0032071140613527745 50-51 7.164829286000878E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 8.700149847286782E-4 60-61 0.0015864979133287662 62-63 0.0013988476225049336 64-65 7.335420459477091E-4 66-67 0.003019463770528942 68-69 0.0026782814235765187 70-71 6.823646939048457E-5 72-73 0.0 74-75 0.0 76-77 0.09121510045773024 78-79 0.003377705234828986 80-81 1.5353205612859028E-4 82-83 0.0 84-85 2.047094081714537E-4 86-87 0.09389338188130676 88-89 0.4415752525431732 90-91 0.38961318110231924 92-93 0.0 94-95 0.0010235470408572684 96-97 0.0 98-99 0.0 100-101 3.2412322960480164E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2930984.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.8147844583054 #Duplication Level Percentage of deduplicated Percentage of total 1 74.39962131351993 40.037995847666195 2 14.321542747436093 15.414214721273606 3 4.83497290671163 7.805790745092982 4 2.167777341616421 4.666338815707439 5 1.2118115217133383 3.2606687922547186 6 0.6982639042085864 2.254615289999993 7 0.45130222212584276 1.7000712266479547 8 0.327233913816072 1.4088018031567677 9 0.2265703033974623 1.0973548837788565 >10 1.2275207653658708 11.944307433281638 >50 0.08730896625862082 3.2424542821641777 >100 0.042520250186181816 4.054564256527989 >500 0.002094229290311599 0.7517806240740188 >1k 0.0012057683791583925 1.2414472113600357 >5k 1.9038448091974618E-4 0.747784879093817 >10k+ 6.346149363991538E-5 0.37180918791991163 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10887 0.3714452211271027 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9192 0.31361481331866703 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7245 0.24718661036703032 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5459 0.1862514432013276 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3397 0.11589964325973802 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3290 0.1122489921473471 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 3.411823469524228E-5 6 0.0 0.0 0.0 0.0 3.411823469524228E-5 7 0.0 0.0 0.0 0.0 3.411823469524228E-5 8 0.0 0.0 0.0 0.0 3.411823469524228E-5 9 0.0 0.0 0.0 0.0 6.823646939048456E-5 10-11 0.0 0.0 0.0 1.705911734762114E-5 6.823646939048456E-5 12-13 0.0 0.0 0.0 3.411823469524228E-5 1.5353205612859025E-4 14-15 0.0 0.0 0.0 6.823646939048456E-5 1.705911734762114E-4 16-17 0.0 0.0 0.0 6.823646939048456E-5 1.705911734762114E-4 18-19 0.0 0.0 0.0 6.823646939048456E-5 1.705911734762114E-4 20-21 0.0 0.0 0.0 1.1941382143334798E-4 2.3882764286669596E-4 22-23 0.0 0.0 0.0 2.2176852551907483E-4 3.070641122571805E-4 24-25 0.0 0.0 0.0 5.629508724714976E-4 3.070641122571805E-4 26-27 0.0 0.0 0.0 0.0011941382143334798 3.411823469524228E-4 28-29 0.0 3.411823469524228E-5 0.0 0.003531237290957576 3.411823469524228E-4 30-31 0.0 3.411823469524228E-5 0.0 0.00958722394936308 3.411823469524228E-4 32-33 0.0 3.411823469524228E-5 0.0 0.02291039459785519 4.094188163429074E-4 34-35 0.0 3.411823469524228E-5 0.0 0.04370545864460536 4.4353705103814965E-4 36-37 0.0 3.411823469524228E-5 0.0 0.07865959008988108 4.605961683857708E-4 38-39 0.0 3.411823469524228E-5 0.0 0.1299222377194826 4.776552857333919E-4 40-41 0.0 3.411823469524228E-5 0.0 0.18614908849724188 4.776552857333919E-4 42-43 0.0 3.411823469524228E-5 0.0 0.24583894009656826 4.776552857333919E-4 44-45 0.0 3.411823469524228E-5 0.0 0.31825489323722 4.947144030810131E-4 46-47 0.0 3.411823469524228E-5 0.0 0.4070987763836309 5.117735204286342E-4 48-49 0.0 3.411823469524228E-5 0.0 0.5036704396885141 5.458917551238765E-4 50-51 0.0 3.411823469524228E-5 0.0 0.6071681046365316 5.458917551238765E-4 52-53 0.0 3.411823469524228E-5 0.0 0.7103928237069872 6.482464592096033E-4 54-55 0.0 3.411823469524228E-5 0.0 0.8308813695332352 6.823646939048456E-4 56-57 0.0 3.411823469524228E-5 0.0 0.9733249993858717 6.994238112524667E-4 58-59 0.0 3.411823469524228E-5 0.0 1.1346701312596725 7.164829286000879E-4 60-61 0.0 3.411823469524228E-5 0.0 1.3141149866392992 7.676602806429513E-4 62-63 0.0 3.411823469524228E-5 0.0 1.4987287545752555 7.847193979905725E-4 64-65 0.0 5.117735204286342E-5 0.0 1.691138539139074 7.847193979905725E-4 66-67 0.0 6.823646939048456E-5 0.0 1.9129241237754966 7.847193979905725E-4 68-69 0.0 6.823646939048456E-5 0.0 2.1700732586735376 7.847193979905725E-4 70-71 0.0 6.823646939048456E-5 0.0 2.4559670063023202 7.847193979905725E-4 72-73 0.0 6.823646939048456E-5 0.0 2.7484114549925893 8.017785153381936E-4 74-75 0.0 1.0235470408572684E-4 0.0 3.0558167495967226 8.188376326858148E-4 76-77 0.0 1.0235470408572684E-4 0.0 3.384409467946601 8.188376326858148E-4 78-79 0.0 1.0235470408572684E-4 0.0 3.767216061227219 8.188376326858148E-4 80-81 0.0 1.0235470408572684E-4 0.0 4.18843978677468 8.358967500334359E-4 82-83 0.0 1.0235470408572684E-4 0.0 4.613280045199837 8.52955867381057E-4 84-85 0.0 1.0235470408572684E-4 0.0 5.05299585395212 8.52955867381057E-4 86-87 0.0 1.0235470408572684E-4 0.0 5.521098033970844 8.52955867381057E-4 88-89 0.0 1.0235470408572684E-4 0.0 6.02640614892473 8.52955867381057E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGTACT 10750 0.0 21.895618 12-13 GTACATG 11985 0.0 21.779345 1 TACATGG 11730 0.0 21.237738 2 GTATCAA 24910 0.0 20.364706 1 GTACTTT 11540 0.0 20.211649 14-15 AGTACTT 11185 0.0 19.962166 12-13 GGTATCA 19670 0.0 19.49368 1 ACATGGG 12155 0.0 19.480162 3 ATCAACG 27785 0.0 17.983116 3 TCAACGC 27935 0.0 17.90354 4 CAACGCA 27940 0.0 17.883352 5 TATCAAC 28380 0.0 17.656252 2 AACGCAG 28445 0.0 17.565859 6 GTATAGG 760 0.0 17.54877 1 ACTTTTT 13745 0.0 17.054697 16-17 AGAGTAC 21040 0.0 16.690527 10-11 CATGGGA 7365 0.0 16.493553 4 TACTTTT 13585 0.0 16.191017 14-15 ACGCAGA 30780 0.0 16.094551 7 CGCAGAG 30725 0.0 15.891703 8 >>END_MODULE