FastQCFastQC Report
Thu 26 May 2016
SRR938930_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938930_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2937520
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108270.3685762139491816No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85560.29126610201802877No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT67280.22903673847327No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53260.1813094038508674No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT36520.12432255780386176No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA34350.1169353740570277No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG116300.022.585551
TACATGG114500.022.0736162
GAGTACT97250.022.04627412-13
ACATGGG117500.020.7446963
GTACTTT103300.020.73288214-15
GTATCAA239250.020.3525621
AGTACTT103050.019.40462912-13
CATGGGA71700.018.681214
GGTATCA188950.018.493591
ATCAACG267900.018.0910993
CAACGCA268050.018.0106545
TCAACGC269000.017.9995254
TATCAAC271600.017.8272192
GTTCGCG4002.3646862E-1117.7481967
AACGCAG272900.017.7070056
GTATAGG8850.017.143811
ACTTTTT126250.017.13432316-17
TTCGCGC4205.0931703E-1116.9030428
TCGCGCA4507.2759576E-1216.8282059
AGAGTAC200450.016.4336310-11
Produced by FastQC (version 0.11.5)