##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938930_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2937520 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.772197295677987 33.0 31.0 34.0 30.0 34.0 2 31.832938328930528 33.0 31.0 34.0 30.0 34.0 3 31.87744900460252 34.0 31.0 34.0 30.0 34.0 4 35.41579019036466 37.0 35.0 37.0 33.0 37.0 5 35.45643706255617 37.0 35.0 37.0 33.0 37.0 6 35.538608077562024 37.0 35.0 37.0 33.0 37.0 7 35.45835126228928 37.0 35.0 37.0 33.0 37.0 8 35.43771957297312 37.0 35.0 37.0 33.0 37.0 9 37.04123103842697 39.0 37.0 39.0 33.0 39.0 10-11 36.92907895095182 39.0 37.0 39.0 33.0 39.0 12-13 36.803318445491435 39.0 37.0 39.0 33.0 39.0 14-15 38.00382959094746 40.0 38.0 41.0 33.0 41.0 16-17 37.93082021569215 40.0 38.0 41.0 33.0 41.0 18-19 37.88387091832566 40.0 38.0 41.0 32.5 41.0 20-21 37.764593092132145 40.0 38.0 41.0 32.0 41.0 22-23 37.848786561453196 40.0 38.0 41.0 32.0 41.0 24-25 37.78973777199815 40.0 38.0 41.0 32.0 41.0 26-27 37.70267827963724 40.0 38.0 41.0 32.0 41.0 28-29 37.57435847245296 40.0 37.0 41.0 32.0 41.0 30-31 37.39029947030148 40.0 37.0 41.0 31.5 41.0 32-33 37.197427251559134 40.0 36.5 41.0 31.0 41.0 34-35 36.88267450093957 40.0 36.0 41.0 30.5 41.0 36-37 36.61696226749095 40.0 36.0 41.0 30.0 41.0 38-39 36.41015107982244 39.0 35.5 41.0 30.0 41.0 40-41 36.25755773577712 39.0 35.0 41.0 30.0 41.0 42-43 36.049694470165306 39.0 35.0 41.0 29.0 41.0 44-45 35.96120877474877 39.0 35.0 41.0 28.0 41.0 46-47 35.73002600833356 39.0 35.0 41.0 27.0 41.0 48-49 35.538723651243224 39.0 35.0 41.0 26.0 41.0 50-51 34.38541065252322 38.0 34.0 40.0 24.5 40.5 52-53 34.525803228573764 38.0 34.0 39.5 24.5 40.5 54-55 35.0186318731447 39.0 35.0 40.5 25.0 41.0 56-57 34.92241840055557 39.0 35.0 41.0 24.5 41.0 58-59 34.724726810370655 38.5 34.0 40.5 24.0 41.0 60-61 34.453916739290285 38.0 34.0 40.0 23.5 41.0 62-63 34.10306738337101 38.0 34.0 40.0 22.5 41.0 64-65 33.69531884038236 37.0 33.5 40.0 22.0 41.0 66-67 33.31605946512704 36.5 33.0 39.5 21.5 41.0 68-69 32.921413811650645 36.0 33.0 39.0 20.0 41.0 70-71 32.504338523652606 35.5 32.0 39.0 18.5 40.5 72-73 32.061904259375254 35.0 32.0 38.0 17.5 40.0 74-75 31.591481419700973 35.0 31.0 37.0 13.5 39.5 76-77 31.17595607859691 35.0 31.0 37.0 9.5 39.0 78-79 30.736802472834228 35.0 31.0 36.0 7.5 39.0 80-81 30.29394165826956 34.5 30.0 36.0 4.5 37.5 82-83 29.897126487649444 34.0 30.0 35.0 2.0 37.0 84-85 29.531876038290804 34.0 30.0 35.0 2.0 37.0 86-87 29.132717053841336 34.0 29.0 35.0 2.0 36.0 88-89 28.705422090743212 34.0 29.0 35.0 2.0 36.0 90-91 28.273062991911544 34.0 29.0 35.0 2.0 35.5 92-93 27.847124274898555 34.0 27.5 35.0 2.0 35.0 94-95 27.362824423323076 34.0 26.5 35.0 2.0 35.0 96-97 26.769745227266537 33.0 25.5 35.0 2.0 35.0 98-99 26.051477096326153 33.0 24.5 35.0 2.0 35.0 100-101 23.36780209156023 30.0 10.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0717527157387039 1101 2 0.18998567057460392 1101 3 0.15853866855617937 1101 4 0.07279998511110364 1101 5 -0.1637610928079951 1101 6 0.03141003746661397 1101 7 0.09506544169799724 1101 8 0.21526035090914064 1101 9 0.2214571697063974 1101 10-11 0.2510104203858461 1101 12-13 0.3634549181393538 1101 14-15 0.06473004743621402 1101 16-17 0.2599111884676262 1101 18-19 0.2572265507935825 1101 20-21 0.46241155876953144 1101 22-23 0.28587300971217644 1101 24-25 0.4568728958632917 1101 26-27 0.4377221123407864 1101 28-29 0.474211108917693 1101 30-31 0.39862630264481425 1101 32-33 0.36456617241164224 1101 34-35 0.28890427843199973 1101 36-37 0.21184694648329838 1101 38-39 0.32319114395351534 1101 40-41 0.1864746800004582 1101 42-43 0.06355055460151249 1101 44-45 0.1766019907371259 1101 46-47 0.16384264523841097 1101 48-49 0.11036981652237188 1101 50-51 0.04471585401021372 1101 52-53 0.15608784201909032 1101 54-55 0.08542181716169495 1101 56-57 0.14039586774152468 1101 58-59 0.04438603122908802 1101 60-61 0.005260886745233506 1101 62-63 -0.024443422998920994 1101 64-65 0.042090484261699146 1101 66-67 -0.1283678240315851 1101 68-69 -0.13977256348237432 1101 70-71 -0.22772595677206908 1101 72-73 -0.3036969355436696 1101 74-75 -0.2888999628090012 1101 76-77 -0.2092224714880757 1101 78-79 -0.2238020360336428 1101 80-81 -0.3535745607525911 1101 82-83 -0.4302071595725181 1101 84-85 -0.46744763420089797 1101 86-87 -0.38299960571480085 1101 88-89 -0.3216081072479646 1101 90-91 -0.40482671797433056 1101 92-93 -0.35515860967114676 1101 94-95 -0.1498025522452231 1101 96-97 -0.32540184599001165 1101 98-99 -0.5215100058675795 1101 100-101 -0.2170253254120702 1102 1 0.2007611792108328 1102 2 0.12379136181808903 1102 3 0.08604725311634098 1102 4 0.05552180623287484 1102 5 0.16892908150590102 1102 6 0.1940723826827906 1102 7 0.210163850844431 1102 8 0.08629088413009356 1102 9 0.15814094909139698 1102 10-11 0.10782877405385705 1102 12-13 0.15096986907490617 1102 14-15 0.4068641632767509 1102 16-17 0.39194301928701236 1102 18-19 0.45598654848814846 1102 20-21 0.4554762943375863 1102 22-23 0.3438018702261587 1102 24-25 0.35167231009329925 1102 26-27 0.41608066617948936 1102 28-29 0.4281010885389449 1102 30-31 0.3109261769036138 1102 32-33 0.33362969921439856 1102 34-35 0.20468477278845398 1102 36-37 0.35724763684373073 1102 38-39 0.23765440933301818 1102 40-41 0.1388252155902947 1102 42-43 0.12376620763617296 1102 44-45 0.33884898812394937 1102 46-47 0.33899259082811284 1102 48-49 0.2592400228373535 1102 50-51 0.3070689488406444 1102 52-53 0.1604727227235827 1102 54-55 0.21519739185396958 1102 56-57 0.20974044833804584 1102 58-59 0.25359720876529934 1102 60-61 0.12936257478649793 1102 62-63 0.1292970737052599 1102 64-65 0.2737991845164558 1102 66-67 0.16933702890197821 1102 68-69 0.06880362891752156 1102 70-71 0.13403169160307726 1102 72-73 0.09003919088704748 1102 74-75 -0.10223350059957781 1102 76-77 -0.06045881207012371 1102 78-79 0.09106922729000644 1102 80-81 -0.07283763165095891 1102 82-83 -0.1668197931262796 1102 84-85 -0.3574637404960974 1102 86-87 -0.14161139746876117 1102 88-89 -0.21710774345508455 1102 90-91 -0.213223538805277 1102 92-93 -0.16404923439023733 1102 94-95 -0.40971888252685673 1102 96-97 -0.6370729349257367 1102 98-99 -0.5274108664915502 1102 100-101 -0.23784480059853763 1103 1 0.2315883288354037 1103 2 0.36120621231325245 1103 3 0.44678104618340697 1103 4 0.47579065646452534 1103 5 0.30214497512331207 1103 6 0.3155566744958307 1103 7 0.3826176603534037 1103 8 0.27638483029979 1103 9 0.4256870516577962 1103 10-11 0.38208404277543195 1103 12-13 0.46851062504825336 1103 14-15 0.4628537245548472 1103 16-17 0.5271880322474942 1103 18-19 0.4894591302926372 1103 20-21 0.5593708768488383 1103 22-23 0.5491082636265361 1103 24-25 0.5736093232187685 1103 26-27 0.5146177297064511 1103 28-29 0.38281117841989953 1103 30-31 0.48858893863938846 1103 32-33 0.5629646644977484 1103 34-35 0.42487111610282113 1103 36-37 0.4492236484197818 1103 38-39 0.5685455499830638 1103 40-41 0.5861954094266011 1103 42-43 0.40195267688963554 1103 44-45 0.6230628197795767 1103 46-47 0.5787913623917831 1103 48-49 0.49433333995806805 1103 50-51 0.34081810531376533 1103 52-53 0.2904109026605539 1103 54-55 0.5343196920345932 1103 56-57 0.5770266301943607 1103 58-59 0.4600124868586093 1103 60-61 0.467192067473043 1103 62-63 0.5290258908850092 1103 64-65 0.3793298115729087 1103 66-67 0.48043795253723687 1103 68-69 0.2723846308367399 1103 70-71 0.1890454188352848 1103 72-73 0.257361564070969 1103 74-75 0.08978767578795654 1103 76-77 0.10858016013237304 1103 78-79 0.265843147863297 1103 80-81 0.20575768711265852 1103 82-83 0.10050287744975606 1103 84-85 0.22911111303504939 1103 86-87 0.30190569359154296 1103 88-89 0.3735398951157407 1103 90-91 0.36838751704405226 1103 92-93 0.29300062029492224 1103 94-95 0.14578625285314573 1103 96-97 0.12839601300568404 1103 98-99 0.2309409062305363 1103 100-101 0.32969650490371905 1104 1 0.1861044124291098 1104 2 0.12852463161356553 1104 3 0.09431369997637162 1104 4 0.11542147652291845 1104 5 0.1101245376905382 1104 6 0.20066014220059003 1104 7 0.253518520876888 1104 8 0.24525616155011676 1104 9 0.26191380984045765 1104 10-11 0.19645030308378608 1104 12-13 0.3169357518218021 1104 14-15 0.2790445635652432 1104 16-17 0.19076791238886415 1104 18-19 0.28139024999759954 1104 20-21 0.2934578511868011 1104 22-23 0.2644868555437654 1104 24-25 0.3346750533831866 1104 26-27 0.2800011236520561 1104 28-29 0.36832505948326144 1104 30-31 0.34601633197032555 1104 32-33 0.3924379780253844 1104 34-35 0.2582381703465373 1104 36-37 0.2132090118372929 1104 38-39 0.2702235067520107 1104 40-41 0.2733635572522388 1104 42-43 0.2437165579530074 1104 44-45 0.28730109002493975 1104 46-47 0.44909453044997605 1104 48-49 0.4894795777289076 1104 50-51 0.45639735799010595 1104 52-53 0.4430002844154046 1104 54-55 0.31970334208837414 1104 56-57 0.3855273088810307 1104 58-59 0.42453894283361393 1104 60-61 0.32423030442433287 1104 62-63 0.22524080406433455 1104 64-65 0.2069637561510973 1104 66-67 0.2879382086454143 1104 68-69 0.28730274024615454 1104 70-71 0.17754059119609877 1104 72-73 0.2596346522234043 1104 74-75 0.34231495130913814 1104 76-77 0.1285398871210539 1104 78-79 0.12527682865006184 1104 80-81 -0.016619701096118433 1104 82-83 -0.09556534976942288 1104 84-85 0.05897255850961969 1104 86-87 0.0980686808373541 1104 88-89 0.05819446920783733 1104 90-91 0.13492506250325675 1104 92-93 0.024024988988255558 1104 94-95 0.013555742685650074 1104 96-97 0.20837590851354904 1104 98-99 0.20171108755512535 1104 100-101 0.5034847290495961 1105 1 0.13765086352531597 1105 2 0.14543097859524678 1105 3 0.21759530974711438 1105 4 0.22481305726173417 1105 5 0.20328151036047615 1105 6 0.17001393136867193 1105 7 0.18885313895677314 1105 8 0.18695953526119524 1105 9 0.31991110686718116 1105 10-11 0.19119276979717625 1105 12-13 0.23878115676724576 1105 14-15 0.36037340028563847 1105 16-17 0.3001872736862907 1105 18-19 0.34312428243328696 1105 20-21 0.2962419136555141 1105 22-23 0.24718444789535 1105 24-25 0.3195150023270301 1105 26-27 0.33862160618284065 1105 28-29 0.39161943329445137 1105 30-31 0.3593695204776921 1105 32-33 0.3504445352383172 1105 34-35 0.35985244604981403 1105 36-37 0.38583052049681754 1105 38-39 0.5234218999845623 1105 40-41 0.5284965785756981 1105 42-43 0.28514775727225583 1105 44-45 0.27087339455992065 1105 46-47 0.37801021270044544 1105 48-49 0.3657614878300137 1105 50-51 0.33610140744450234 1105 52-53 0.3234830654745693 1105 54-55 0.2631476308059675 1105 56-57 0.1959386363412321 1105 58-59 0.2935098107883576 1105 60-61 0.4152381630688424 1105 62-63 0.35562059683638125 1105 64-65 0.30010696367937584 1105 66-67 0.25544421525268746 1105 68-69 0.2541198640823765 1105 70-71 0.13822747176471495 1105 72-73 0.32273080799475906 1105 74-75 0.37874302218710554 1105 76-77 0.3449683334119733 1105 78-79 0.25779797691314954 1105 80-81 0.1632277561068065 1105 82-83 0.17674619292754556 1105 84-85 0.18495563989914743 1105 86-87 0.19609450924860639 1105 88-89 0.19811250404616132 1105 90-91 0.31040934521423935 1105 92-93 0.36532128991928303 1105 94-95 0.41806908235929185 1105 96-97 0.09953193013733497 1105 98-99 0.31040427506138357 1105 100-101 0.48453363284377104 1106 1 0.25877627811557957 1106 2 0.13003814244209977 1106 3 0.25905043750865886 1106 4 0.1780307552831033 1106 5 0.1413415057016678 1106 6 0.18071676846722795 1106 7 0.30405050975031855 1106 8 0.2636795670736589 1106 9 0.24383214880329973 1106 10-11 0.17333834202676002 1106 12-13 0.31779185156350565 1106 14-15 0.28506855522032026 1106 16-17 0.3370583952022912 1106 18-19 0.29567422575758684 1106 20-21 0.31281906716136376 1106 22-23 0.347067076088166 1106 24-25 0.2741597046845996 1106 26-27 0.27273805987299937 1106 28-29 0.3579559953068241 1106 30-31 0.3136241205655992 1106 32-33 0.3114062871849299 1106 34-35 0.22712849420904746 1106 36-37 0.2551673368919367 1106 38-39 0.3284946670937501 1106 40-41 0.37547536929277214 1106 42-43 0.36527551881133746 1106 44-45 0.3983012390414302 1106 46-47 0.32728522188649833 1106 48-49 0.2707154211622438 1106 50-51 0.3470011231401102 1106 52-53 0.2519507115960167 1106 54-55 0.24458512233872653 1106 56-57 0.36926538756173954 1106 58-59 0.45606586514634984 1106 60-61 0.5035751285848704 1106 62-63 0.4298968243305765 1106 64-65 0.29575821273763125 1106 66-67 0.3378334003799708 1106 68-69 0.3961376796461167 1106 70-71 0.4533147318676569 1106 72-73 0.3840281551043887 1106 74-75 0.3529418140152778 1106 76-77 0.38393701606999286 1106 78-79 0.5716038205038281 1106 80-81 0.45325636685539905 1106 82-83 0.5163986875840472 1106 84-85 0.6066161110627668 1106 86-87 0.6977776019579593 1106 88-89 0.7314661262712185 1106 90-91 0.60522359220721 1106 92-93 0.6372772739077881 1106 94-95 0.7523941591370722 1106 96-97 0.6230098246669336 1106 98-99 0.766094407642548 1106 100-101 1.1011218242214653 1107 1 0.10380145676408858 1107 2 0.05351231634262987 1107 3 0.1518013847054327 1107 4 0.0846702449958272 1107 5 0.06995212389743699 1107 6 0.07142368899861395 1107 7 -0.004498720502425613 1107 8 0.15454187583582524 1107 9 0.1252881940769086 1107 10-11 0.15705375135964772 1107 12-13 0.11543944640308013 1107 14-15 0.08400146011697274 1107 16-17 0.20313244440856693 1107 18-19 0.2241673435936633 1107 20-21 0.12491105315724127 1107 22-23 0.15329843189844183 1107 24-25 0.18530928983638972 1107 26-27 0.2254691039476242 1107 28-29 0.1253570791876939 1107 30-31 0.20308529748756854 1107 32-33 0.22121876620272474 1107 34-35 0.08690812108545032 1107 36-37 0.1400747753840932 1107 38-39 0.14508188113624243 1107 40-41 0.1637145424739117 1107 42-43 0.29691237076087873 1107 44-45 0.35134665652739727 1107 46-47 0.27814009695244124 1107 48-49 0.2138145530983664 1107 50-51 0.2257877520787659 1107 52-53 0.10027860953856305 1107 54-55 0.038841273122862674 1107 56-57 0.12321819558054159 1107 58-59 0.0248345093360669 1107 60-61 0.017740156641082194 1107 62-63 0.09741444938453725 1107 64-65 0.22177902708705943 1107 66-67 0.0654874697291632 1107 68-69 0.1442670259604455 1107 70-71 0.21477581287097536 1107 72-73 0.10866174581946098 1107 74-75 0.20031987741750967 1107 76-77 0.18497461618509448 1107 78-79 0.32018446411803936 1107 80-81 0.2500243875738306 1107 82-83 0.3424149457970813 1107 84-85 0.2964602432386805 1107 86-87 0.46917705522158926 1107 88-89 0.41636565147384985 1107 90-91 0.31833639255252066 1107 92-93 0.38592769834786367 1107 94-95 0.5371579594344205 1107 96-97 0.5621813272327607 1107 98-99 0.64584286095414 1107 100-101 0.903767980281124 1108 1 0.26776789600436146 1108 2 0.32461133902380723 1108 3 0.22906796531887252 1108 4 0.2926910207042255 1108 5 0.28731705105952443 1108 6 0.2755837480065466 1108 7 0.2953500716790245 1108 8 0.22509446050605675 1108 9 0.3586301327332464 1108 10-11 0.37959201431373657 1108 12-13 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-2.541213106249838 2316 94-95 -2.645174416044508 2316 96-97 -2.7562669486293103 2316 98-99 -3.295828594984556 2316 100-101 -3.8660787630138955 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9592.0 3 2578.0 4 2540.0 5 2959.0 6 3341.0 7 4177.0 8 5176.0 9 6236.0 10 7783.0 11 9822.0 12 12875.0 13 18406.0 14 17992.0 15 17012.0 16 21018.0 17 27475.0 18 23871.0 19 17902.0 20 17707.0 21 18624.0 22 20538.0 23 22472.0 24 25406.0 25 29347.0 26 34211.0 27 40011.0 28 47294.0 29 55867.0 30 66953.0 31 81535.0 32 100817.0 33 128793.0 34 172466.0 35 244718.0 36 376477.0 37 588191.0 38 560465.0 39 94843.0 40 30.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.36094825649789 19.277385235005635 14.587923398287803 22.77374311020867 2 15.03452961260531 20.657461268537798 40.90153481223448 23.406474306622414 3 19.422953147780735 25.071918754550822 30.09156331593245 25.413564781735992 4 12.662945635205562 16.842580195338815 38.05423155157061 32.44024261788501 5 13.480018552047458 37.63755147051557 34.38167395764858 14.500756019788398 6 30.83326661294966 36.458765951140236 17.963671176964603 14.7442962589455 7 27.617196963269546 33.27728309323727 21.296569827525406 17.808950115967775 8 25.450244943879945 35.14636325289834 20.205195440616187 19.19819636260553 9 26.142869750073856 17.41516620911556 21.07749451671257 35.36446952409801 10-11 24.586364694125713 26.735741885981422 27.624119552451727 21.053773867441134 12-13 25.93581334382321 24.32309724356267 27.879580638956746 21.861508773657377 14-15 22.89398461921421 25.549612549837963 26.76202887888462 24.79437395206321 16-17 21.61879087795524 28.68336015940273 27.4428815968621 22.25496736577993 18-19 21.50069113757684 27.700637332265178 29.546834218474437 21.251837311683538 20-21 22.709074422346706 26.59483150792155 29.298463941628622 21.397630128103117 22-23 22.15006259722507 26.60089825782559 29.23025435415801 22.018784790791333 24-25 22.0496010530876 26.723035166431536 29.477880422612067 21.749483357868794 26-27 21.95083584047698 26.614131547198355 29.678945293679647 21.75608731864502 28-29 21.683374417500918 27.060404377274505 29.56562120028255 21.690600004942027 30-31 21.925616195683066 26.840118000936535 29.36525761243045 21.869008190949945 32-33 21.743542889702653 26.870006507333827 29.36383697893406 22.022613624029457 34-35 22.19525913664743 26.82920036672682 29.240381365821623 21.73515913080413 36-37 22.229536521485148 26.563358704915906 29.466464004459574 21.74064076913937 38-39 21.964909674052198 26.89516568700938 29.427821833208405 21.712102805730016 40-41 22.096470890191213 26.893698934850498 29.270941523719895 21.738888651238398 42-43 21.857089871372704 26.95891289728661 28.973139931115565 22.210857300225125 44-45 21.988610343821072 27.005999556602895 28.859690495123914 22.14569960445212 46-47 22.259202124236037 27.199742139217438 28.462890231828208 22.07816550471832 48-49 22.16824794260631 27.21959332552826 28.47882133505187 22.13333739681357 50-51 22.030643764906166 27.39750558240256 28.536824168914094 22.03502648377718 52-53 22.22053108775025 27.525964240552597 28.14480531998566 22.108699351711493 54-55 21.989694786056138 27.327767534039577 28.270383560138313 22.41215411976597 56-57 22.166564040357297 27.482635388860153 28.066167785287732 22.28463278549481 58-59 22.330483044325653 27.22701681748664 28.138456723118555 22.304043415069152 60-61 22.130839113809998 27.275748406107247 28.536119227761613 22.05729325232114 62-63 22.143240928757475 27.181847296150696 28.56285788279734 22.11205389229449 64-65 21.930574217218965 27.34619417075749 28.418058093798628 22.305173518224922 66-67 21.945794189582653 27.338820907857585 28.074567582073012 22.64081732048675 68-69 21.939830729507836 27.363381149053488 28.298100472094823 22.398687649343852 70-71 22.225279937967375 27.14064267058115 28.1023504034929 22.531726987958574 72-73 22.070109360300634 27.091409156429442 28.233420011979533 22.605061471290394 74-75 21.918659205632764 27.69016732532893 27.892526180968886 22.498647288069424 76-77 22.093820738517397 27.19245533425988 28.021689236223462 22.69203469099926 78-79 22.099417631931402 27.250135857356195 28.093760831147492 22.556685679564914 80-81 22.11217275472986 27.435828580924955 27.849806266726706 22.60219239761848 82-83 22.186813585596045 27.271055975810825 28.09256221208254 22.449568226510593 84-85 22.107112042401084 27.25452634318036 27.898674281331743 22.739687333086817 86-87 22.02042928790205 27.171641041187407 28.079923216853675 22.728006454056867 88-89 22.18340747416256 27.075760605471267 27.892296977848147 22.848534942518032 90-91 22.126807174660616 27.01106717905763 28.003870327919717 22.85825531836204 92-93 22.22591903712793 27.208108684760017 27.89641262070919 22.669559657402864 94-95 22.177160915042865 27.062412402856534 27.882266014491574 22.878160667609027 96-97 21.99409568038799 26.893044008773092 28.101903878526013 23.010956432312906 98-99 22.106624574564048 26.996560895304228 27.72498190035021 23.171832629781512 100-101 22.405852207117867 26.438763168279202 28.114493303191203 23.040891321411728 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3028.0 1 1754.0 2 544.0 3 589.0 4 521.5 5 569.5 6 936.0 7 1799.0 8 2905.0 9 3527.5 10 3456.5 11 3342.0 12 3590.0 13 3870.5 14 4194.0 15 4548.0 16 4803.0 17 5021.0 18 5290.0 19 5617.5 20 6065.0 21 6839.0 22 7843.0 23 9359.5 24 11270.5 25 13790.0 26 18069.5 27 23321.0 28 27212.0 29 30681.5 30 35075.0 31 40905.5 32 46917.0 33 52746.0 34 59566.0 35 65782.0 36 73880.0 37 81637.0 38 85442.0 39 87953.0 40 89891.5 41 91838.5 42 93730.5 43 96417.5 44 100520.5 45 103827.0 46 105724.0 47 111400.0 48 128138.5 49 127927.0 50 109587.0 51 100901.5 52 97132.5 53 94203.5 54 90257.0 55 84380.5 56 76896.0 57 69059.0 58 63161.5 59 56824.5 60 48293.5 61 39444.5 62 31288.5 63 23760.0 64 16901.5 65 11993.5 66 8659.0 67 6394.0 68 4723.5 69 3480.0 70 2520.0 71 1666.5 72 1114.5 73 810.0 74 603.5 75 449.0 76 298.5 77 163.0 78 93.0 79 59.0 80 42.0 81 34.5 82 27.5 83 27.5 84 24.0 85 15.0 86 9.0 87 9.0 88 10.5 89 9.5 90 7.5 91 5.0 92 4.5 93 3.0 94 3.0 95 3.0 96 1.5 97 1.0 98 2.0 99 1.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2786704430948555 2 0.0852079304991966 3 0.08888450121190665 4 0.10311419156295105 5 0.10461205370516627 6 0.08119093657234674 7 0.09031427871129388 8 0.08721642746262154 9 0.09167597156785316 10-11 0.08534409978485252 12-13 0.08941215719382337 14-15 0.09819507611863068 16-17 0.08728451210544949 18-19 0.09754827201176502 20-21 0.09977804406438083 22-23 0.11181200468422343 24-25 0.12496936191072743 26-27 0.12719913396334323 28-29 0.11923323075247146 30-31 0.11254391459462404 32-33 0.10684182575778209 34-35 0.37756338680247287 36-37 0.5807960456439445 38-39 0.5622259593126174 40-41 0.5240815381682508 42-43 0.5532047441379122 44-45 0.5748897028786187 46-47 0.5638770119011957 48-49 0.585493885999074 50-51 0.5773067076990114 52-53 0.5807960456439445 54-55 0.5707365396661129 56-57 0.5418516299463493 58-59 0.536830387537787 60-61 0.48251586372177896 62-63 0.5594685312780849 64-65 0.5946002069773142 66-67 0.6070767177755385 68-69 0.6084554317928048 70-71 0.6047958822408017 72-73 0.5970682752798279 74-75 0.5943789318881233 76-77 0.5784811677877938 78-79 0.5534430403878101 80-81 0.5483026498542989 82-83 0.563196165472916 84-85 0.5732726926114545 86-87 0.5639621177047305 88-89 0.5770854326098206 90-91 0.5907704458182412 92-93 0.598770391350527 94-95 0.5973576350118468 96-97 0.5941916991203464 98-99 0.5982597565293173 100-101 0.5757407609139682 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2937520.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.64555261315388 #Duplication Level Percentage of deduplicated Percentage of total 1 76.1077813948812 42.35059553879276 2 13.38660538699602 14.898101087472323 3 4.4910663866522915 7.49723612722881 4 2.0766527904175764 4.622259684337363 5 1.0979854878847455 3.0549004617285016 6 0.675673012600381 2.2558918903165615 7 0.4162163793645482 1.6212413305470463 8 0.2829817700677913 1.2597341579896555 9 0.2170985906220622 1.0872513942031365 >10 1.1235072961431165 11.377829440153189 >50 0.0828678430453128 3.1887969487937498 >100 0.0382524497170559 3.7936690080546285 >500 0.0019008807380224717 0.6976068729311352 >1k 0.0011650559359509453 1.2222195247765788 >5k 1.8395620041330714E-4 0.7032368622458204 >10k+ 6.131873347110239E-5 0.3694296704286995 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10827 0.3685762139491816 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8556 0.29126610201802877 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6728 0.22903673847327 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5326 0.1813094038508674 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3652 0.12432255780386176 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3435 0.1169353740570277 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 3.404232141398186E-5 3 0.0 0.0 0.0 0.0 3.404232141398186E-5 4 0.0 0.0 0.0 0.0 3.404232141398186E-5 5 0.0 0.0 0.0 0.0 3.404232141398186E-5 6 0.0 0.0 0.0 0.0 3.404232141398186E-5 7 0.0 0.0 0.0 0.0 3.404232141398186E-5 8 0.0 0.0 0.0 0.0 3.404232141398186E-5 9 0.0 0.0 0.0 0.0 3.404232141398186E-5 10-11 0.0 0.0 0.0 3.404232141398186E-5 3.404232141398186E-5 12-13 0.0 0.0 0.0 5.106348212097279E-5 1.1914812494893651E-4 14-15 0.0 0.0 0.0 6.808464282796372E-5 1.8723276777690023E-4 16-17 0.0 0.0 0.0 1.5319044636291838E-4 2.0425392848389117E-4 18-19 0.0 0.0 0.0 2.0425392848389117E-4 2.723385713118549E-4 20-21 0.0 0.0 0.0 3.0638089272583675E-4 2.723385713118549E-4 22-23 0.0 0.0 0.0 4.0850785696778234E-4 3.0638089272583675E-4 24-25 0.0 0.0 0.0 5.787194640376916E-4 3.5744437484680957E-4 26-27 0.0 0.0 0.0 0.0011233966066614015 4.0850785696778234E-4 28-29 0.0 0.0 0.0 0.003999972766142869 4.0850785696778234E-4 30-31 0.0 3.404232141398186E-5 0.0 0.010723331245404286 4.425501783817642E-4 32-33 0.0 3.404232141398186E-5 0.0 0.024170048203927125 4.425501783817642E-4 34-35 0.0 3.404232141398186E-5 0.0 0.04582096462321959 4.765924997957461E-4 36-37 0.0 3.404232141398186E-5 0.0 0.08117391541163975 4.765924997957461E-4 38-39 0.0 3.404232141398186E-5 0.0 0.13227143985402653 4.765924997957461E-4 40-41 0.0 3.404232141398186E-5 0.0 0.1879306353658869 4.765924997957461E-4 42-43 0.0 3.404232141398186E-5 0.0 0.2460578991802609 4.765924997957461E-4 44-45 0.0 3.404232141398186E-5 0.0 0.31953824995234076 4.765924997957461E-4 46-47 0.0 3.404232141398186E-5 0.0 0.4099376344671696 5.446771426237099E-4 48-49 0.0 5.106348212097279E-5 0.0 0.5081837740679213 5.787194640376916E-4 50-51 0.0 6.808464282796372E-5 0.0 0.6142426264331817 6.468041068656554E-4 52-53 0.0 8.510580353495464E-5 0.0 0.7243695362074131 6.638252675726464E-4 54-55 0.0 1.0212696424194558E-4 0.0 0.8483857131185489 7.148887496936191E-4 56-57 0.0 1.1914812494893651E-4 0.0 0.9941719545739263 7.489310711076009E-4 58-59 0.0 1.3616928565592744E-4 0.0 1.1585112611999238 7.489310711076009E-4 60-61 0.0 1.3616928565592744E-4 0.0 1.343888722459762 7.489310711076009E-4 62-63 0.0 1.3616928565592744E-4 0.0 1.5310363844331274 7.489310711076009E-4 64-65 0.0 1.7021160706990932E-4 0.0 1.7274435578310956 7.659522318145919E-4 66-67 0.0 1.7021160706990932E-4 0.0 1.9509484190745936 7.829733925215828E-4 68-69 0.0 1.7021160706990932E-4 0.0 2.21167855878428 7.829733925215828E-4 70-71 0.0 1.7021160706990932E-4 0.0 2.495693646341131 8.510580353495466E-4 72-73 0.0 1.7021160706990932E-4 0.0 2.7920150330891365 9.191426781775103E-4 74-75 0.0 1.7021160706990932E-4 0.0 3.1015278193850593 9.531849995914922E-4 76-77 0.0 1.7021160706990932E-4 0.0 3.4336787494212806 9.87227321005474E-4 78-79 0.0 1.7021160706990932E-4 0.0 3.811293199705874 9.87227321005474E-4 80-81 0.0 1.7021160706990932E-4 0.0 4.229843541490782 0.001004248481712465 82-83 0.0 1.7021160706990932E-4 0.0 4.665670361393284 0.0010382908031264467 84-85 0.0 1.7021160706990932E-4 0.0 5.11494389825431 0.0010553119638334377 86-87 0.0 1.7021160706990932E-4 0.0 5.593051281352978 0.0010723331245404287 88-89 0.0 1.7021160706990932E-4 0.0 6.110375418720554 0.0010893542852474195 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 11630 0.0 22.58555 1 TACATGG 11450 0.0 22.073616 2 GAGTACT 9725 0.0 22.046274 12-13 ACATGGG 11750 0.0 20.744696 3 GTACTTT 10330 0.0 20.732882 14-15 GTATCAA 23925 0.0 20.352562 1 AGTACTT 10305 0.0 19.404629 12-13 CATGGGA 7170 0.0 18.68121 4 GGTATCA 18895 0.0 18.49359 1 ATCAACG 26790 0.0 18.091099 3 CAACGCA 26805 0.0 18.010654 5 TCAACGC 26900 0.0 17.999525 4 TATCAAC 27160 0.0 17.827219 2 GTTCGCG 400 2.3646862E-11 17.748196 7 AACGCAG 27290 0.0 17.707005 6 GTATAGG 885 0.0 17.14381 1 ACTTTTT 12625 0.0 17.134323 16-17 TTCGCGC 420 5.0931703E-11 16.903042 8 TCGCGCA 450 7.2759576E-12 16.828205 9 AGAGTAC 20045 0.0 16.43363 10-11 >>END_MODULE