FastQCFastQC Report
Thu 26 May 2016
SRR938930_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938930_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2937520
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107750.3668060132356546No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92260.3140744573653967No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT72290.24609194150167485No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56680.1929518777744492No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT34710.11816089762793104No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA33420.11376943816552738No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG122500.022.5579871
TACATGG120850.022.1066072
GAGTACT99000.022.00226612-13
ACATGGG123350.020.7352853
GTACTTT104250.020.62111314-15
AGTACTT103100.020.29877512-13
GTATCAA251450.019.7814121
GGTATCA199350.018.1159631
CAACGCA284900.017.3054735
ATCAACG285200.017.287273
CATGGGA70600.017.2738344
TATCAAC287400.017.2374942
TCAACGC286700.017.1968214
AACGCAG289950.017.0040666
AGAGTAC204150.016.79493710-11
ACTTTTT129850.016.75608416-17
TACTTTT128100.016.00385314-15
ATGGGCG4208.749339E-1015.8175465
ACGCAGA308950.015.7125927
TTTGCGT4852.5465852E-1115.6544793
Produced by FastQC (version 0.11.5)