FastQCFastQC Report
Thu 26 May 2016
SRR938929_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938929_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2721927
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT160340.5890679654524166No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137390.5047526990988369No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103060.3786288170108897No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT93450.3433229473090204No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT69890.2567666215883086No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63830.23450298262958558No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC56330.20694897401730464No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT49990.18365665207038984No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC41580.1527594237464855No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT40640.14930598800041295No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC40260.14790991823072405No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA40040.14710166731143046No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG36070.13251641208599643No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA36060.13247967340784672No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC33900.12454411892750981No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA32470.11929048795210158No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACT109600.022.91561512-13
GTACATG140550.022.8267271
TACATGG143800.021.7712272
TTTGCGT8300.021.684323
GTACTTT119750.020.91417914-15
CTTTGCG9300.020.3712352
AGTACTT113300.020.34959212-13
ACATGGG145700.020.2195343
GTAAGCG3450.019.30181994-95
TTGCGTT9450.019.0454884
CATGGGG51250.018.3907554
ACTTTTT143250.017.86576516-17
GTATAGG7300.016.9035641
CATGGGA87500.016.617694
AGAGTAC241750.016.56687410-11
TACTTTT139600.016.51597214-15
GTGTAGC14550.015.9830951
GTATCAA404100.015.4089411
ATGGGGA28600.015.235695
GTGTAAG10950.015.1698661
Produced by FastQC (version 0.11.5)