FastQCFastQC Report
Thu 26 May 2016
SRR938929_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938929_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2721927
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT171020.6283048737163047No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131430.4828564469216111No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108500.3986146579243308No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT88460.32499034691231615No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67750.24890454446427107No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT66230.24332026538551546No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC53820.19772756580172796No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT52230.1918861159759244No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC45350.16660990540892537No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC43860.1611358423646189No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA41150.15117966058604804No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT40370.14831404369037082No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG39880.14651384846103513No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA39210.1440523570250047No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC33180.12189893410073085No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA32060.11778420214796355No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA31330.11510227864303488No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAAGCG3650.022.75244594-95
GAGTACT118100.022.01859712-13
GTACATG134900.021.437711
AGTACTT122600.021.15235112-13
TACATGG138300.020.6206952
CTTTGCG7450.020.3819032
GTACTTT128450.020.26289614-15
ACATGGG136250.019.920973
TAGGACG2953.0595402E-919.3023534
TTTGCGT8000.018.9806463
CATGGGG46700.018.8993594
CTCCGAC5150.018.88989494-95
TTGCGTT9250.017.4416754
ACTTTTT153250.017.09187116-17
TACTTTT152250.017.0018614-15
AGAGTAC251800.016.91366410-11
CATGGGA87250.016.2069684
TAAAGCG2751.13988535E-515.529625
GTATCAA411900.015.1639571
GGATATG11000.015.1592251
Produced by FastQC (version 0.11.5)