##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938929_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2721927 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.18875855230504 33.0 31.0 34.0 31.0 34.0 2 32.42415465220044 34.0 31.0 34.0 31.0 34.0 3 32.507848667506515 34.0 31.0 34.0 30.0 34.0 4 36.05398418105996 37.0 35.0 37.0 35.0 37.0 5 35.984705320899494 37.0 35.0 37.0 35.0 37.0 6 36.03359384730009 37.0 35.0 37.0 35.0 37.0 7 35.973897169174634 37.0 35.0 37.0 35.0 37.0 8 35.94935683433097 37.0 35.0 37.0 35.0 37.0 9 37.61980905439418 39.0 37.0 39.0 35.0 39.0 10-11 37.53567784881814 39.0 37.0 39.0 35.0 39.0 12-13 37.44986493024978 39.0 37.0 39.0 34.0 39.0 14-15 38.733046661427736 40.0 38.0 41.0 34.0 41.0 16-17 38.83577774128402 40.0 38.0 41.0 35.0 41.0 18-19 38.90516718486572 40.0 38.0 41.0 35.0 41.0 20-21 38.885310664099364 40.0 38.0 41.0 35.0 41.0 22-23 38.811252285605015 40.0 38.0 41.0 34.0 41.0 24-25 38.72794714920716 40.0 38.0 41.0 34.0 41.0 26-27 38.56135836853817 40.0 38.0 41.0 34.0 41.0 28-29 38.41019211756965 40.0 38.0 41.0 34.0 41.0 30-31 38.204157385558105 40.0 38.0 41.0 34.0 41.0 32-33 38.076772080955884 40.0 37.0 41.0 33.0 41.0 34-35 37.959537673126434 40.0 37.0 41.0 33.0 41.0 36-37 37.815614452555124 40.0 37.0 41.0 33.0 41.0 38-39 37.657814298473106 40.0 37.0 41.0 33.0 41.0 40-41 37.48969002475085 40.0 37.0 41.0 32.5 41.0 42-43 37.286727197312786 40.0 37.0 41.0 32.0 41.0 44-45 37.0000336158905 40.0 36.0 41.0 31.0 41.0 46-47 36.63439926934117 40.0 36.0 41.0 30.5 41.0 48-49 36.25196469266075 39.0 36.0 41.0 30.0 41.0 50-51 35.98314300861118 39.0 35.0 41.0 29.0 41.0 52-53 35.73223620618775 39.0 35.0 40.0 28.0 41.0 54-55 35.448132885268414 39.0 35.0 40.0 27.5 41.0 56-57 35.31038874297511 38.5 35.0 40.0 27.5 41.0 58-59 35.045194268619255 38.0 34.5 40.0 26.5 41.0 60-61 34.66962229332381 38.0 34.0 40.0 26.0 41.0 62-63 34.267654863631535 37.5 34.0 40.0 25.5 41.0 64-65 33.86696961380669 37.0 33.0 39.5 24.5 41.0 66-67 33.404758467071304 36.0 33.0 39.0 23.0 40.5 68-69 32.90992245567203 36.0 32.0 39.0 22.0 40.0 70-71 32.36874170394724 35.0 31.5 38.0 21.0 40.0 72-73 31.844459825704362 35.0 31.0 37.5 19.5 39.5 74-75 31.32085118373858 35.0 31.0 37.0 18.0 39.0 76-77 29.409408848951493 32.5 28.5 35.0 13.0 37.0 78-79 30.37107314046262 34.0 30.0 36.0 13.0 38.0 80-81 30.334479580091603 34.0 30.0 35.5 10.0 37.0 82-83 29.91369441575766 34.0 30.0 35.0 7.0 37.0 84-85 29.449978636458656 34.0 29.0 35.0 2.0 36.0 86-87 28.769514575519473 34.0 29.0 35.0 2.0 36.0 88-89 28.071531124824432 33.0 27.5 35.0 2.0 35.5 90-91 27.48061355062057 33.0 26.0 35.0 2.0 35.0 92-93 27.08037981915018 33.0 26.0 34.5 2.0 35.0 94-95 26.5261180773768 32.0 25.0 34.0 2.0 35.0 96-97 25.659110071651444 32.0 23.5 34.0 2.0 35.0 98-99 24.440515671434245 32.0 10.0 34.0 2.0 35.0 100-101 18.8033560415103 24.0 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1310096550231563 1101 2 0.1705824955119155 1101 3 0.2012588833686877 1101 4 0.09074720364850464 1101 5 0.16855147715698138 1101 6 0.1717469100059219 1101 7 0.19891955761460167 1101 8 0.24102155448099438 1101 9 0.26138187126338863 1101 10-11 0.3303090300949876 1101 12-13 0.28825975128333425 1101 14-15 0.3278987657576451 1101 16-17 0.28314393258205683 1101 18-19 0.2991755220144299 1101 20-21 0.26437839241090444 1101 22-23 0.23885125642750893 1101 24-25 0.28270110967255135 1101 26-27 0.23643272088224876 1101 28-29 0.24437745500305397 1101 30-31 0.20086727332369492 1101 32-33 0.4352902266194789 1101 34-35 0.48431057510915565 1101 36-37 0.538698405137076 1101 38-39 0.4977724010541422 1101 40-41 0.45579444975593475 1101 42-43 0.519671103059757 1101 44-45 0.8035874330156432 1101 46-47 0.7343232910132542 1101 48-49 0.7180963673082132 1101 50-51 0.7478565640332135 1101 52-53 0.7124392082604274 1101 54-55 0.7746139544337112 1101 56-57 0.76943005229883 1101 58-59 0.7227393779277236 1101 60-61 0.8198601949194497 1101 62-63 0.8419667337082402 1101 64-65 0.7668000341921015 1101 66-67 0.7453355169208038 1101 68-69 0.8698710970179206 1101 70-71 0.9141020813998253 1101 72-73 0.9542296700638211 1101 74-75 0.8711613350012293 1101 76-77 0.4303875881097561 1101 78-79 0.8762136826394773 1101 80-81 0.887161997843922 1101 82-83 0.87603843862086 1101 84-85 1.054612297817009 1101 86-87 1.273479553128567 1101 88-89 1.2473416414263312 1101 90-91 1.0843831864011086 1101 92-93 1.0445102119826224 1101 94-95 0.9232032886177066 1101 96-97 1.012603671770183 1101 98-99 1.351137383885142 1101 100-101 1.3242471061686096 1102 1 0.17147670731361586 1102 2 0.1172475777165829 1102 3 0.1586380333476498 1102 4 0.10134243677318722 1102 5 0.1259163348605128 1102 6 0.11625048628652479 1102 7 0.20963073481632932 1102 8 0.18293276893899701 1102 9 0.15407546736047095 1102 10-11 0.22840639100223115 1102 12-13 0.247575424288911 1102 14-15 0.31160621412077916 1102 16-17 0.24007512410818066 1102 18-19 0.19705480528090646 1102 20-21 0.26797627201534624 1102 22-23 0.29338744800181615 1102 24-25 0.25059142529095624 1102 26-27 0.26379407778124175 1102 28-29 0.366114159172227 1102 30-31 0.29059201052247374 1102 32-33 0.28274379278307293 1102 34-35 0.32992300714055034 1102 36-37 0.41810789328090436 1102 38-39 0.2573455758984551 1102 40-41 0.39125703771767206 1102 42-43 0.47300714496663687 1102 44-45 0.36152141233841206 1102 46-47 0.5460786875516348 1102 48-49 0.507582471683584 1102 50-51 0.42125221596973716 1102 52-53 0.37131318572038907 1102 54-55 0.49235315734226504 1102 56-57 0.5121919783194429 1102 58-59 0.4027193526833983 1102 60-61 0.42905277771026107 1102 62-63 0.46086208534639184 1102 64-65 0.5105404993173437 1102 66-67 0.39455466086167945 1102 68-69 0.477544523342047 1102 70-71 0.5034272772361135 1102 72-73 0.5677124245867695 1102 74-75 0.6712804105318959 1102 76-77 0.2877027440240312 1102 78-79 0.6143926058690816 1102 80-81 0.6643634900217137 1102 82-83 0.7643956138248882 1102 84-85 0.9725345703209172 1102 86-87 1.2093776540223935 1102 88-89 1.0146703830316284 1102 90-91 0.9504732373715363 1102 92-93 0.9815294535606185 1102 94-95 1.3500686714419246 1102 96-97 1.2985150211215704 1102 98-99 1.3305110610467317 1102 100-101 1.4561293185486086 1103 1 0.12544115064638817 1103 2 0.14525338478124894 1103 3 0.09534115748139271 1103 4 0.05261415041166373 1103 5 0.14919197574565146 1103 6 0.14061462946728653 1103 7 0.18045809970602278 1103 8 0.15677809266608733 1103 9 0.1660699439446205 1103 10-11 0.1519391853168841 1103 12-13 0.14235751109318073 1103 14-15 0.217701053388609 1103 16-17 0.09074675935644905 1103 18-19 0.1924761333504037 1103 20-21 0.23708159855313937 1103 22-23 0.22855580646170637 1103 24-25 0.20322997161660794 1103 26-27 0.15141338756055234 1103 28-29 0.10120599691994414 1103 30-31 0.13540823188199624 1103 32-33 0.2347832921687214 1103 34-35 0.21174040951056838 1103 36-37 0.12635736756362803 1103 38-39 0.029054540618993485 1103 40-41 0.17583914566666436 1103 42-43 0.08515640354733733 1103 44-45 -0.018997329756487602 1103 46-47 -0.025139564733350994 1103 48-49 0.0729156558864048 1103 50-51 -0.06583725073183189 1103 52-53 0.02782431184482448 1103 54-55 0.15488182843471776 1103 56-57 0.10389612101026557 1103 58-59 0.0577013538772988 1103 60-61 -0.04748640385978575 1103 62-63 0.023873378436839232 1103 64-65 0.09206567895084561 1103 66-67 -0.02173429088836798 1103 68-69 0.10028113479603462 1103 70-71 0.007846557876952431 1103 72-73 -0.02936509018553224 1103 74-75 0.07092143362735115 1103 76-77 -0.12707029825124394 1103 78-79 0.05965628322114114 1103 80-81 0.08863393239781558 1103 82-83 0.16754550159021164 1103 84-85 0.30637197122111814 1103 86-87 0.339470439414999 1103 88-89 0.10621013834585113 1103 90-91 0.27957064108243657 1103 92-93 0.48874186912674844 1103 94-95 0.5802649886489633 1103 96-97 0.551732823436403 1103 98-99 0.8001915822385186 1103 100-101 0.7743091441561667 1104 1 0.029139676627863764 1104 2 -0.009751033467928494 1104 3 0.01361188400890967 1104 4 -0.002085473132744653 1104 5 0.043352336956793636 1104 6 0.04449183579999527 1104 7 0.03760992232425053 1104 8 -0.01109115358280377 1104 9 0.07024348283165693 1104 10-11 0.07407403172884841 1104 12-13 0.03154281991920982 1104 14-15 0.09511815341279828 1104 16-17 0.048099959134610515 1104 18-19 0.07581681732494161 1104 20-21 0.08783246497982589 1104 22-23 0.0704594002331902 1104 24-25 0.11130018921970475 1104 26-27 0.014047834301067041 1104 28-29 0.03776110266379362 1104 30-31 7.572515465881224E-4 1104 32-33 0.07687689110242246 1104 34-35 0.1341523992137965 1104 36-37 0.15025562860744657 1104 38-39 0.12444664804363015 1104 40-41 0.14289188877182113 1104 42-43 0.16843230016565514 1104 44-45 0.21151644546048232 1104 46-47 0.24939823355352075 1104 48-49 0.27980980759671326 1104 50-51 0.23846521798259346 1104 52-53 0.2525867346119739 1104 54-55 0.430509875713291 1104 56-57 0.34733119099288245 1104 58-59 0.38213044970753174 1104 60-61 0.27825605546762944 1104 62-63 0.29178160091023386 1104 64-65 0.38664650522792954 1104 66-67 0.3552243902241443 1104 68-69 0.37337627799355744 1104 70-71 0.32476557383896676 1104 72-73 0.49754619973875336 1104 74-75 0.4712281787162347 1104 76-77 0.19779739807143315 1104 78-79 0.5450676459702528 1104 80-81 0.3880138046224886 1104 82-83 0.3268172501819251 1104 84-85 0.5577650016178524 1104 86-87 0.7190316250094604 1104 88-89 0.8032634225253119 1104 90-91 0.6577824439892019 1104 92-93 0.7288758353492426 1104 94-95 0.9256722744661161 1104 96-97 0.734507565710441 1104 98-99 0.8995648325945425 1104 100-101 1.0033816798851554 1105 1 -0.033313149007490495 1105 2 0.02374339151441518 1105 3 0.03150084323826974 1105 4 0.06837255665178077 1105 5 0.005081775536133648 1105 6 -0.038904927907680076 1105 7 0.05728955327406737 1105 8 0.03763976589024054 1105 9 0.04103525630546301 1105 10-11 0.06868562459039396 1105 12-13 0.10070330329432409 1105 14-15 0.04345724392216965 1105 16-17 -0.020793092169704153 1105 18-19 0.051683577263986535 1105 20-21 0.056051381048192184 1105 22-23 -0.035840370476449834 1105 24-25 -0.04311572593299218 1105 26-27 -0.06595495537060003 1105 28-29 0.09108473538698547 1105 30-31 0.14096094311090468 1105 32-33 0.11075212599718753 1105 34-35 0.15741580204556982 1105 36-37 0.09358158200960531 1105 38-39 0.10587878776276938 1105 40-41 0.1782124255362234 1105 42-43 0.3046920516845333 1105 44-45 0.22493206449515668 1105 46-47 0.23336240251291684 1105 48-49 0.1781988253363309 1105 50-51 0.29802425254978715 1105 52-53 0.1992780519790145 1105 54-55 0.38942566177916405 1105 56-57 0.28052826854620605 1105 58-59 0.1541770897392567 1105 60-61 0.06228894930878681 1105 62-63 0.13908904412759426 1105 64-65 0.16756211553538236 1105 66-67 0.15573251115868914 1105 68-69 0.16271243175880556 1105 70-71 0.4196323958550252 1105 72-73 0.4300288602902036 1105 74-75 0.25598133299555315 1105 76-77 0.22361635566614524 1105 78-79 0.26968106471590403 1105 80-81 0.2961864801376848 1105 82-83 0.376429086646457 1105 84-85 0.3606449505720235 1105 86-87 0.4238625076157341 1105 88-89 0.4285932920922235 1105 90-91 0.39921395534922155 1105 92-93 0.7313047317335801 1105 94-95 0.8807002781858841 1105 96-97 0.9162434426941211 1105 98-99 1.0452697020286514 1105 100-101 1.1565900527249262 1106 1 0.01940851615844963 1106 2 0.04538492377218262 1106 3 0.07085659347515616 1106 4 0.057483687232661396 1106 5 0.10335033221021916 1106 6 0.07766360866673239 1106 7 0.11621045848091427 1106 8 0.09572355281603251 1106 9 0.07035442219108745 1106 10-11 0.11178271190470213 1106 12-13 0.17819395105600933 1106 14-15 0.09543846066659256 1106 16-17 -0.018397107981442673 1106 18-19 0.03322552410513424 1106 20-21 0.12471731489891624 1106 22-23 0.07889968478661302 1106 24-25 0.18314585990918175 1106 26-27 0.175559337853457 1106 28-29 0.16310562137765316 1106 30-31 0.23758007490806676 1106 32-33 0.2338046848034523 1106 34-35 0.08150978900361139 1106 36-37 0.05903679544532281 1106 38-39 0.1927881573766328 1106 40-41 0.2914561962939217 1106 42-43 0.2831460849086298 1106 44-45 0.416025994761803 1106 46-47 0.36278692602916607 1106 48-49 0.37011617960080656 1106 50-51 0.3172710522473352 1106 52-53 0.36620989199739284 1106 54-55 0.41104356035459233 1106 56-57 0.41186910821192413 1106 58-59 0.35165498562000863 1106 60-61 0.31076980418546185 1106 62-63 0.3906896923236616 1106 64-65 0.24808023064245788 1106 66-67 0.33801424379473843 1106 68-69 0.2642090843900533 1106 70-71 0.3596144754865449 1106 72-73 0.39397247518339995 1106 74-75 0.49188023384526147 1106 76-77 0.2699646572437153 1106 78-79 0.42188116911640705 1106 80-81 0.4110469861929893 1106 82-83 0.37947289878150414 1106 84-85 0.4878617494817803 1106 86-87 0.6848746623964779 1106 88-89 0.8563518250663655 1106 90-91 1.0441675015427627 1106 92-93 0.9990479948456752 1106 94-95 1.0208807040486434 1106 96-97 0.9050079459339955 1106 98-99 0.8748475131668165 1106 100-101 1.3712389600168358 1107 1 0.02294290401405874 1107 2 0.05249932785120848 1107 3 0.025026881440858517 1107 4 0.006285037252943937 1107 5 0.06831426407185148 1107 6 -0.017849601832139683 1107 7 0.03297055466261156 1107 8 -0.006399976936563689 1107 9 -0.05042531591656285 1107 10-11 0.00391399123721925 1107 12-13 -0.002044257994342047 1107 14-15 0.02421330277795164 1107 16-17 0.09477754002828931 1107 18-19 0.04602247062579323 1107 20-21 0.013756366987678348 1107 22-23 0.00562523377554669 1107 24-25 0.07103587381497078 1107 26-27 0.02114678498249134 1107 28-29 0.0029826420625127525 1107 30-31 0.052194913542983556 1107 32-33 0.1677311869182958 1107 34-35 0.17478213966123946 1107 36-37 0.19872912987953129 1107 38-39 0.07929048949320361 1107 40-41 0.1199779096029161 1107 42-43 0.0953752117410076 1107 44-45 0.09294673176858481 1107 46-47 0.07690492243683877 1107 48-49 -0.0031006927556447295 1107 50-51 -0.010704569167579336 1107 52-53 -0.11236650292699579 1107 54-55 -0.19566851740194124 1107 56-57 0.030499243213945704 1107 58-59 0.05229160998990068 1107 60-61 0.04828284086197954 1107 62-63 0.02175391968329876 1107 64-65 -0.041889495297624535 1107 66-67 -0.042706684416621954 1107 68-69 -0.023266290776525977 1107 70-71 0.19152391404034574 1107 72-73 0.14543584212237803 1107 74-75 0.029437812499875093 1107 76-77 0.017032491417186435 1107 78-79 0.2483351206779112 1107 80-81 0.07611050377801476 1107 82-83 0.036304117276198866 1107 84-85 0.4265097012750445 1107 86-87 0.29975711502988744 1107 88-89 0.44386703452230236 1107 90-91 0.5234627243250749 1107 92-93 0.5726429308211074 1107 94-95 0.7529980051668979 1107 96-97 0.9567027138811532 1107 98-99 0.7866496888432728 1107 100-101 0.7222507509664879 1108 1 0.11984143926876101 1108 2 0.11361315694449559 1108 3 0.13464409071560368 1108 4 0.06789255074401979 1108 5 -0.010548482770346368 1108 6 0.09432572284318752 1108 7 0.09609618474029702 1108 8 0.1512439772827321 1108 9 0.0072860936784096 1108 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-0.7739551532980897 2316 58-59 -0.6775249876216023 2316 60-61 -0.6705106571205306 2316 62-63 -0.46561541711633225 2316 64-65 -0.67903203327797 2316 66-67 -0.7213615304642289 2316 68-69 -0.6144952695725294 2316 70-71 -0.4826880446890378 2316 72-73 -0.47922311338442825 2316 74-75 -0.7430343961821215 2316 76-77 -0.7238960635696792 2316 78-79 -0.6368246201621233 2316 80-81 -0.7334734388655768 2316 82-83 -0.72799165546461 2316 84-85 -0.9244861824956345 2316 86-87 -1.0536545146719583 2316 88-89 -1.1498943054186626 2316 90-91 -1.3416314141102141 2316 92-93 -1.2571611420794824 2316 94-95 -1.0949794232729957 2316 96-97 -0.9622265248872246 2316 98-99 -1.2234897416547348 2316 100-101 -0.48960134912287856 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 11.0 4 24.0 5 58.0 6 161.0 7 367.0 8 871.0 9 1610.0 10 2926.0 11 4361.0 12 6500.0 13 9081.0 14 11035.0 15 14955.0 16 20400.0 17 29364.0 18 27978.0 19 18326.0 20 16273.0 21 17112.0 22 18478.0 23 20335.0 24 23880.0 25 27728.0 26 33088.0 27 39328.0 28 48003.0 29 58263.0 30 70139.0 31 85492.0 32 105835.0 33 134162.0 34 179497.0 35 257950.0 36 402997.0 37 610950.0 38 405218.0 39 19169.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.51752146840971 20.35205240947856 15.495617788532476 21.634808333579247 2 15.14114816451727 22.165326255994376 39.51039098403447 23.183134595453883 3 19.42660475464625 26.150958493743587 31.08882053045508 23.333616221155086 4 12.61058801356539 18.099383267809902 38.455954182459706 30.834074536165 5 12.783700665006814 38.591409688797675 34.441114695581476 14.183774950614032 6 29.434294159982983 37.63466103242298 17.83060309846664 15.100441709127393 7 26.44369228124046 34.37924676157737 21.62912524839939 17.547935708782784 8 25.3626566766853 34.26432817632508 20.362632796544506 20.01038235044511 9 25.83221372211672 17.68934288098101 21.73441829997645 34.74402509692582 10-11 24.2818047655209 27.15085672760511 27.602117911317976 20.965220595556016 12-13 26.189295304392807 25.05675207307176 27.79279165091496 20.961160971620473 14-15 22.70130324357866 26.090313350852952 27.65754522298937 23.550838182579014 16-17 20.77742030177147 29.409841281387994 28.090762166032725 21.721976250807813 18-19 20.649400127557392 28.376101064251895 30.433845767240086 20.540653040950627 20-21 22.195378494720835 26.719599754144767 29.936309827559665 21.14871192357473 22-23 21.534357828112217 26.932298331292497 30.055508468816395 21.47783537177889 24-25 21.875886321587227 27.059094963709494 30.19639784211462 20.868620872588654 26-27 21.48949990209142 26.762730962292526 30.82648799912709 20.921281136488965 28-29 20.768999315558425 27.76112291034991 30.523393904391998 20.946483869699666 30-31 21.30700051838275 27.0269739048843 30.200773202220336 21.465252374512616 32-33 21.18570777247149 27.247075325679198 30.258287602863703 21.308929298985607 34-35 21.925562329809274 27.020362076573928 30.045003286694964 21.009072306921826 36-37 21.755546924006065 26.67236373431209 30.473493569026083 21.098595772655766 38-39 21.403243967370365 27.223749387546793 30.384182457870473 20.988824187212362 40-41 21.41723289150436 27.291274800868337 30.18947888209609 21.102013425531215 42-43 21.163242609315773 27.427436033376797 29.492433419270025 21.9168879380374 44-45 21.57990065689891 27.148092494893234 29.34499316648787 21.927013681719988 46-47 21.991812681801356 27.653057569319877 28.926766946159706 21.428362802719064 48-49 21.88771829660177 27.551879857809503 28.956608972271162 21.603792873317566 50-51 21.282805796006734 27.880694740473462 29.215493467175698 21.62100599634411 52-53 21.712228138374027 28.356032325628128 28.226767286558385 21.70497224943946 54-55 21.213574059848042 27.663030639690188 28.698528652678785 22.424866647782988 56-57 21.8507513243375 27.852785912333434 28.304928824321884 21.991533939007184 58-59 22.325822164060277 27.413036913158518 28.393564500117936 21.86757642266327 60-61 21.811140233118113 27.65498283907972 29.114671392808354 21.4192055349938 62-63 21.583292170078426 27.588945236675094 29.021757160615312 21.806005432631167 64-65 21.279791645847908 28.103452583191768 28.66107695201594 21.955678818944385 66-67 21.453921880195235 27.728966932356546 28.201954923957828 22.61515626349039 68-69 21.2045277944637 27.71828403790797 28.633854485268316 22.44333368236001 70-71 21.72672553795997 27.63289163024791 28.299570175756035 22.340812656036082 72-73 21.593158082490824 27.185078806301565 28.775826831505768 22.445936279701844 74-75 21.068713451903744 28.2716803205964 28.116312450701287 22.54329377679857 76-77 21.260723343125466 27.430393076016614 28.411543232882597 22.897340347975323 78-79 21.236901749666494 27.523106671129344 28.573520124122236 22.666471455081926 80-81 21.40097534165247 27.618741637330736 28.356713905415226 22.623569115601573 82-83 21.213849599934164 27.435122249788478 28.66952346627959 22.681504683997773 84-85 20.92521003077788 27.479217354645474 28.457055628879957 23.138516985696693 86-87 20.93947806120629 27.418132421550506 28.509307885198034 23.133081632045176 88-89 21.015311580759906 27.198767869999728 28.178589471687545 23.607331077552818 90-91 20.87313473987562 26.974661546267008 28.525849036734495 23.626354677122876 92-93 21.132418319815336 27.361479569437385 28.410038182508202 23.096063928239076 94-95 20.77542257760352 27.0204983914708 28.44413812207775 23.75994090884793 96-97 20.219168258369898 26.951659614677396 28.824634165427653 24.004537961525052 98-99 20.427311239427066 27.111564711324 28.134387880350943 24.32673616889799 100-101 20.44628212426529 26.022769668583884 29.20588314714977 24.325065060001055 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1191.0 1 830.0 2 532.5 3 611.0 4 559.5 5 575.5 6 950.5 7 1925.0 8 3133.0 9 3793.0 10 3595.5 11 3312.0 12 3481.0 13 3737.0 14 4041.5 15 4309.5 16 4478.0 17 4733.5 18 5053.5 19 5440.5 20 6081.0 21 6984.0 22 8171.0 23 9536.0 24 11249.5 25 13749.5 26 17439.0 27 21762.5 28 25185.0 29 29078.0 30 34495.5 31 40464.5 32 46688.5 33 52904.0 34 60130.0 35 66423.5 36 73227.0 37 80411.5 38 82879.5 39 82461.0 40 82236.5 41 84639.5 42 87861.5 43 91066.0 44 95281.5 45 98102.0 46 100181.0 47 112963.5 48 144941.5 49 144368.0 50 111720.0 51 93743.5 52 83482.0 53 77961.0 54 73375.0 55 66651.5 56 59996.0 57 53526.5 58 47612.5 59 42020.0 60 35750.5 61 28872.5 62 22352.0 63 16575.5 64 11887.5 65 8752.0 66 6110.5 67 4431.0 68 3177.5 69 2097.0 70 1480.0 71 1041.0 72 759.5 73 571.5 74 462.0 75 379.0 76 243.0 77 109.0 78 53.5 79 29.5 80 19.0 81 11.5 82 7.5 83 5.5 84 3.0 85 3.0 86 3.0 87 2.5 88 1.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4029130832678467 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 9.552056318924057E-4 16-17 0.0024247527578807222 18-19 5.510801722456186E-4 20-21 0.0 22-23 0.0 24-25 1.1021603444912373E-4 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.012785059996098353 36-37 0.023659708728411895 38-39 0.010672586002490148 40-41 0.0063374219808246144 42-43 0.005731233791354434 44-45 0.0017267178730362718 46-47 0.0012674843961649228 48-49 0.0031411569818000262 50-51 6.796655457695963E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 9.184669537426978E-4 60-61 0.0016532405167368558 62-63 0.0015246551432128784 64-65 8.44989597443282E-4 66-67 0.0030125716082760484 68-69 0.0026451848267789696 70-71 1.8369339074853955E-5 72-73 0.0 74-75 0.0 76-77 0.09432655614937506 78-79 0.0034534357460725435 80-81 3.306481033473712E-4 82-83 0.0 84-85 1.653240516736856E-4 86-87 0.09427144813215049 88-89 0.4463014621626517 90-91 0.39716348013741737 92-93 0.0 94-95 0.0011205296835660912 96-97 0.0 98-99 0.0 100-101 6.061881894701806E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2721927.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.837961537242954 #Duplication Level Percentage of deduplicated Percentage of total 1 70.66770593937258 32.39263586774156 2 15.39526896036231 14.11375492921196 3 5.771719015452049 7.936915027021945 4 2.6866886383467614 4.9260932186834605 5 1.5135776018792122 3.468965594928588 6 0.9222974000910973 2.536573965076494 7 0.5873627500752399 1.8846457802450665 8 0.40799313848312296 1.4961259031398741 9 0.31568658833503355 1.3023386724532249 >10 1.507297759163535 12.513599307963627 >50 0.13916566425766091 4.408377109580318 >100 0.0777842065135922 6.435874005368864 >500 0.004166898480945447 1.2260500334632882 >1k 0.002644377882127884 2.642000263838466 >5k 4.006633154739219E-4 1.206582508436628 >10k+ 2.403979892843531E-4 1.5094678128467602 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 17102 0.6283048737163047 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13143 0.4828564469216111 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10850 0.3986146579243308 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 8846 0.32499034691231615 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6775 0.24890454446427107 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 6623 0.24332026538551546 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 5382 0.19772756580172796 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 5223 0.1918861159759244 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 4535 0.16660990540892537 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 4386 0.1611358423646189 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 4115 0.15117966058604804 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 4037 0.14831404369037082 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 3988 0.14651384846103513 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 3921 0.1440523570250047 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 3318 0.12189893410073085 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3206 0.11778420214796355 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 3133 0.11510227864303488 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 7.347735629941582E-5 0.0 0.0 0.0 0.0 6 7.347735629941582E-5 0.0 0.0 0.0 0.0 7 1.1021603444912373E-4 0.0 0.0 0.0 0.0 8 1.1021603444912373E-4 0.0 0.0 0.0 0.0 9 1.1021603444912373E-4 0.0 0.0 1.1021603444912373E-4 0.0 10-11 1.1021603444912373E-4 0.0 0.0 1.285853735239777E-4 0.0 12-13 1.1021603444912373E-4 0.0 0.0 2.571707470479554E-4 0.0 14-15 1.1021603444912373E-4 0.0 0.0 2.755400861228093E-4 0.0 16-17 1.1021603444912373E-4 0.0 0.0 3.857561205719331E-4 0.0 18-19 1.1021603444912373E-4 0.0 0.0 5.143414940959108E-4 0.0 20-21 1.1021603444912373E-4 0.0 0.0 6.061881894701806E-4 0.0 22-23 1.1021603444912373E-4 0.0 0.0 9.552056318924056E-4 0.0 24-25 1.8369339074853955E-4 0.0 0.0 0.0011388990226409452 0.0 26-27 1.8369339074853955E-4 0.0 0.0 0.001965519281009373 0.0 28-29 1.8369339074853955E-4 0.0 0.0 0.005125045601884253 0.0 30-31 1.8369339074853955E-4 0.0 0.0 0.013758634967065614 1.8369339074853955E-5 32-33 1.8369339074853955E-4 0.0 0.0 0.031007444358353474 3.673867814970791E-5 34-35 1.8369339074853955E-4 0.0 0.0 0.059241118516404005 3.673867814970791E-5 36-37 1.8369339074853955E-4 0.0 0.0 0.10534815959428744 3.673867814970791E-5 38-39 2.571707470479554E-4 0.0 0.0 0.1709083307524412 3.673867814970791E-5 40-41 2.571707470479554E-4 0.0 0.0 0.24638794501101607 3.673867814970791E-5 42-43 2.939094251976633E-4 0.0 0.0 0.32693749685425066 3.673867814970791E-5 44-45 3.306481033473712E-4 0.0 0.0 0.4243317326291264 3.673867814970791E-5 46-47 4.04125459646787E-4 0.0 0.0 0.5432915724778806 3.673867814970791E-5 48-49 4.4086413779649493E-4 0.0 0.0 0.6694338239049027 3.673867814970791E-5 50-51 4.4086413779649493E-4 0.0 0.0 0.8035299991513365 3.673867814970791E-5 52-53 4.4086413779649493E-4 0.0 0.0 0.9391508295409833 5.5108017224561866E-5 54-55 4.4086413779649493E-4 0.0 0.0 1.0961168319356105 1.1021603444912373E-4 56-57 4.4086413779649493E-4 0.0 0.0 1.2869742649233429 1.4695471259883164E-4 58-59 4.4086413779649493E-4 0.0 0.0 1.492361845119285 1.4695471259883164E-4 60-61 4.7760281594620284E-4 0.0 0.0 1.7192231826937312 1.4695471259883164E-4 62-63 4.7760281594620284E-4 0.0 0.0 1.953468994576269 1.4695471259883164E-4 64-65 4.7760281594620284E-4 0.0 0.0 2.1985894551911205 1.4695471259883164E-4 66-67 4.7760281594620284E-4 0.0 0.0 2.4805771793292033 1.4695471259883164E-4 68-69 5.143414940959108E-4 0.0 0.0 2.8084515124762714 1.4695471259883164E-4 70-71 5.510801722456186E-4 0.0 0.0 3.1709336804403643 1.8369339074853955E-4 72-73 6.061881894701806E-4 0.0 0.0 3.5306604475432293 1.8369339074853955E-4 74-75 6.245575285450344E-4 0.0 0.0 3.9249032027677453 1.8369339074853955E-4 76-77 6.245575285450344E-4 0.0 0.0 4.327834655374667 1.8369339074853955E-4 78-79 6.245575285450344E-4 0.0 0.0 4.803306627988187 1.8369339074853955E-4 80-81 6.980348848444503E-4 0.0 0.0 5.326079648719455 1.8369339074853955E-4 82-83 6.980348848444503E-4 0.0 0.0 5.847089212899538 1.8369339074853955E-4 84-85 7.164042239193042E-4 0.0 0.0 6.392309565980278 1.8369339074853955E-4 86-87 7.347735629941582E-4 0.0 0.0 6.969639523763863 1.8369339074853955E-4 88-89 7.347735629941582E-4 0.0 0.0 7.588502557195692 1.8369339074853955E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTAAGCG 365 0.0 22.752445 94-95 GAGTACT 11810 0.0 22.018597 12-13 GTACATG 13490 0.0 21.43771 1 AGTACTT 12260 0.0 21.152351 12-13 TACATGG 13830 0.0 20.620695 2 CTTTGCG 745 0.0 20.381903 2 GTACTTT 12845 0.0 20.262896 14-15 ACATGGG 13625 0.0 19.92097 3 TAGGACG 295 3.0595402E-9 19.302353 4 TTTGCGT 800 0.0 18.980646 3 CATGGGG 4670 0.0 18.899359 4 CTCCGAC 515 0.0 18.889894 94-95 TTGCGTT 925 0.0 17.441675 4 ACTTTTT 15325 0.0 17.091871 16-17 TACTTTT 15225 0.0 17.00186 14-15 AGAGTAC 25180 0.0 16.913664 10-11 CATGGGA 8725 0.0 16.206968 4 TAAAGCG 275 1.13988535E-5 15.52962 5 GTATCAA 41190 0.0 15.163957 1 GGATATG 1100 0.0 15.159225 1 >>END_MODULE