FastQCFastQC Report
Thu 26 May 2016
SRR938928_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938928_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2731670
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT160180.5863812246720871No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135490.4959969542441071No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104080.3810123477579649No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT93650.3428305761676923No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT70400.25771780632360425No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64660.236705019273924No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC57310.20979840171030908No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT51600.18889543758945992No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA41480.15184850293044183No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT40160.14701629406187422No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC39510.14463679727053413No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC39490.14456358198464678No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG37330.1366563311088089No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA35310.12926158723418274No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC33860.12395347900734716No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA32050.11732749563453859No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGCGT7800.023.6618043
GAGTACT104700.023.54967112-13
TAGATCG1050.00224476422.5354655
GTACATG142950.022.1923921
CTTTGCG8650.021.8837532
AGTACTT110500.021.58549712-13
TACATGG146450.021.3594462
GTACTTT115300.021.2828214-15
TTGCGTT8600.020.9108164
GTCTACG2309.1939E-820.617421
ACATGGG147500.020.3732173
GTATAGG7750.019.5798971
CATGGGA90300.018.395234
ACTTTTT138200.018.3573616-17
TACTTTT138200.017.27678114-15
CATGGGG52250.017.20894
AGAGTAC240250.016.910910-11
GTGTAGC14950.016.8111271
ATGGGAT33200.015.9648855
AGTACAT112050.015.8801242
Produced by FastQC (version 0.11.5)