##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938928_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2731670 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.863504010367283 33.0 31.0 34.0 30.0 34.0 2 31.924314064290343 33.0 31.0 34.0 30.0 34.0 3 31.96305849535266 34.0 31.0 34.0 30.0 34.0 4 35.50003770587223 37.0 35.0 37.0 33.0 37.0 5 35.544447901832946 37.0 35.0 37.0 33.0 37.0 6 35.634638883906185 37.0 35.0 37.0 33.0 37.0 7 35.55720896008669 37.0 35.0 37.0 33.0 37.0 8 35.543015444764556 37.0 35.0 37.0 33.0 37.0 9 37.15640102940692 39.0 37.0 39.0 33.0 39.0 10-11 37.05263080826015 39.0 37.0 39.0 33.0 39.0 12-13 36.93952874981238 39.0 37.0 39.0 33.0 39.0 14-15 38.134363411393025 40.0 38.0 41.0 33.0 41.0 16-17 38.083672808208895 40.0 38.0 41.0 33.0 41.0 18-19 38.069114131648405 40.0 38.0 41.0 33.0 41.0 20-21 37.967091925452195 40.0 38.0 41.0 33.0 41.0 22-23 38.02929197157783 40.0 38.0 41.0 33.0 41.0 24-25 37.95625203630014 40.0 38.0 41.0 33.0 41.0 26-27 37.86827032547855 40.0 38.0 41.0 33.0 41.0 28-29 37.745247229716625 40.0 38.0 41.0 32.0 41.0 30-31 37.56060376985507 40.0 37.0 41.0 32.0 41.0 32-33 37.36006911522988 40.0 37.0 41.0 31.5 41.0 34-35 37.03786712889917 40.0 36.0 41.0 30.5 41.0 36-37 36.76247240698913 40.0 36.0 41.0 30.0 41.0 38-39 36.554374613331774 39.0 36.0 41.0 30.0 41.0 40-41 36.41668008946908 39.0 35.0 41.0 30.0 41.0 42-43 36.19228878305212 39.0 35.0 41.0 30.0 41.0 44-45 36.07168160868626 39.0 35.0 41.0 29.0 41.0 46-47 35.79285089340953 39.0 35.0 41.0 27.0 41.0 48-49 35.56597356196026 39.0 35.0 41.0 26.5 41.0 50-51 34.393468647384196 38.0 34.0 40.0 24.5 40.5 52-53 34.53958494254431 38.5 34.5 39.5 24.0 40.5 54-55 35.04792416360688 39.0 35.0 40.5 25.0 41.0 56-57 34.954998773643965 39.0 35.0 41.0 24.5 41.0 58-59 34.77166184055907 39.0 35.0 40.5 24.0 41.0 60-61 34.49277877635293 38.0 34.0 40.0 23.0 41.0 62-63 34.16148528189716 38.0 34.0 40.0 22.5 41.0 64-65 33.76394256992975 37.0 34.0 40.0 22.0 41.0 66-67 33.37906573634444 37.0 33.0 39.5 20.5 41.0 68-69 32.98876694476273 36.0 33.0 39.0 19.5 41.0 70-71 32.5565375759151 36.0 32.5 39.0 18.5 40.5 72-73 32.113125487339246 35.0 32.0 38.0 16.5 40.0 74-75 31.649902623669767 35.0 31.5 37.0 12.5 39.5 76-77 31.233188306054537 35.0 31.0 37.0 9.0 39.0 78-79 30.791940827405945 35.0 31.0 36.0 7.0 39.0 80-81 30.33840123440972 34.5 30.5 36.0 2.0 37.5 82-83 29.9472093993784 34.0 30.0 35.5 2.0 37.0 84-85 29.58459605296394 34.0 30.0 35.0 2.0 37.0 86-87 29.20498980477144 34.0 29.5 35.0 2.0 36.0 88-89 28.779354387609047 34.0 29.0 35.0 2.0 36.0 90-91 28.33870892164866 34.0 29.0 35.0 2.0 35.5 92-93 27.928521746770294 34.0 28.5 35.0 2.0 35.0 94-95 27.458082967561968 34.0 27.0 35.0 2.0 35.0 96-97 26.880795630511738 34.0 25.5 35.0 2.0 35.0 98-99 26.213774174772208 33.0 24.5 35.0 2.0 35.0 100-101 23.678049691214532 31.0 11.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2893315807305683 1101 2 0.35897029377436596 1101 3 0.3211898223326628 1101 4 0.22541126802192224 1101 5 0.17104414914285115 1101 6 0.3192451760903978 1101 7 0.17547276008845358 1101 8 0.26911082086984095 1101 9 0.2699061880658178 1101 10-11 0.43254495517087577 1101 12-13 0.4504579571415519 1101 14-15 0.24893398873808792 1101 16-17 0.45729836004193913 1101 18-19 0.40499007829671996 1101 20-21 0.6053916932086238 1101 22-23 0.4247969357371275 1101 24-25 0.4188312166786332 1101 26-27 0.40704283676029007 1101 28-29 0.4002450122819212 1101 30-31 0.48815319746002217 1101 32-33 0.39580021942265375 1101 34-35 0.20512180462779384 1101 36-37 0.21073997079596296 1101 38-39 0.18627432819640433 1101 40-41 0.16837192857907723 1101 42-43 0.026560087347192507 1101 44-45 0.23271308254127376 1101 46-47 0.2832312461125497 1101 48-49 0.22408939722848942 1101 50-51 0.03476000052526018 1101 52-53 0.08144320521022763 1101 54-55 -0.0277870158907092 1101 56-57 0.04998844886582532 1101 58-59 -0.09374960436932867 1101 60-61 -0.03512044976024953 1101 62-63 -0.14239621321272722 1101 64-65 -0.03391421138808681 1101 66-67 -0.23630639728625624 1101 68-69 -0.19461011614284018 1101 70-71 -0.3026273378418338 1101 72-73 -0.39420888949003796 1101 74-75 -0.33303515913479487 1101 76-77 -0.27420067261863323 1101 78-79 -0.3490675298368373 1101 80-81 -0.37808443438866135 1101 82-83 -0.42261247261010126 1101 84-85 -0.5030649465622936 1101 86-87 -0.45358237133228485 1101 88-89 -0.44165056871169384 1101 90-91 -0.410370381541032 1101 92-93 -0.47760334520652137 1101 94-95 -0.4967100415565966 1101 96-97 -0.6880974014719214 1101 98-99 -0.6741992649658854 1101 100-101 -0.31319594334386736 1102 1 0.20626759242964 1102 2 0.1716511588847318 1102 3 0.22508461146090752 1102 4 0.25358425348833435 1102 5 0.29368213364044493 1102 6 0.23254952682528085 1102 7 0.2757421682362775 1102 8 0.2397588394560728 1102 9 0.41395591922570674 1102 10-11 0.2494773535124537 1102 12-13 0.4132289352853107 1102 14-15 0.46912883864646915 1102 16-17 0.5038230201812084 1102 18-19 0.5641489296971542 1102 20-21 0.6085150384013431 1102 22-23 0.5715417740007211 1102 24-25 0.549783868125651 1102 26-27 0.5525584234275343 1102 28-29 0.5158313481733217 1102 30-31 0.45543653328642364 1102 32-33 0.5533844990138377 1102 34-35 0.48841061003602704 1102 36-37 0.4618134062121655 1102 38-39 0.5645900965098107 1102 40-41 0.4872444158755229 1102 42-43 0.4925437189955346 1102 44-45 0.625851979372186 1102 46-47 0.6640734455837674 1102 48-49 0.6255534539465302 1102 50-51 0.5214485650850733 1102 52-53 0.4586270774545298 1102 54-55 0.49710191675819715 1102 56-57 0.413845185710521 1102 58-59 0.39134166683192717 1102 60-61 0.369482099850039 1102 62-63 0.4098818903457868 1102 64-65 0.4683775882154251 1102 66-67 0.5286612655310066 1102 68-69 0.4431384086921284 1102 70-71 0.3761109408367105 1102 72-73 0.4142169710413057 1102 74-75 0.26594772017780244 1102 76-77 0.3516585648587949 1102 78-79 0.31676964249159667 1102 80-81 0.12575905768625262 1102 82-83 0.07472642371588734 1102 84-85 0.18649817800418234 1102 86-87 0.2327269169214965 1102 88-89 0.3249298507407268 1102 90-91 0.19425422690671112 1102 92-93 0.11312640468602453 1102 94-95 0.18764677134217678 1102 96-97 -0.03785746822278924 1102 98-99 -0.0013891016168798842 1102 100-101 0.38216701447168333 1103 1 0.017564795601220595 1103 2 0.16657698817469324 1103 3 0.2512570947567667 1103 4 0.1818353387644578 1103 5 0.1481292767954585 1103 6 0.10217644730849429 1103 7 0.17973209597219864 1103 8 0.07498800130920102 1103 9 0.12161089628040855 1103 10-11 0.19181425214926406 1103 12-13 0.31630898743116376 1103 14-15 0.3606669721781657 1103 16-17 0.37372255113153585 1103 18-19 0.3457940505098449 1103 20-21 0.2718692883069451 1103 22-23 0.3445511775355925 1103 24-25 0.5021740115079538 1103 26-27 0.37087775289501934 1103 28-29 0.24163241438315453 1103 30-31 0.25769039491582646 1103 32-33 0.28533468056630795 1103 34-35 0.3624706792006549 1103 36-37 0.23311834860941616 1103 38-39 0.3368532846575292 1103 40-41 0.3245379973900384 1103 42-43 0.11543773856524808 1103 44-45 0.16858654647334248 1103 46-47 0.2001726128656287 1103 48-49 0.04934039606951757 1103 50-51 0.07903418026898379 1103 52-53 0.15004095133145512 1103 54-55 0.20314189399134364 1103 56-57 0.24551655790153148 1103 58-59 0.12675994192383655 1103 60-61 0.12003929592106743 1103 62-63 0.12416334456882794 1103 64-65 -0.04745095975932401 1103 66-67 0.0962883851302152 1103 68-69 0.038761284866396295 1103 70-71 0.02820222237357939 1103 72-73 0.028217690488745006 1103 74-75 -0.04534408244188981 1103 76-77 -0.018046781030118808 1103 78-79 -0.021173883702488183 1103 80-81 -0.06331637722254158 1103 82-83 -0.03783187876385696 1103 84-85 0.06896174071539107 1103 86-87 0.04342724170599155 1103 88-89 -0.05167787007609448 1103 90-91 -0.05736711269313943 1103 92-93 -0.08449505137790325 1103 94-95 -0.07980311623241931 1103 96-97 -0.2778899278877063 1103 98-99 -0.3135912494398454 1103 100-101 -0.12083217027979032 1104 1 0.17181758009622428 1104 2 0.15943817091149626 1104 3 0.1870879007795594 1104 4 0.21523146518787684 1104 5 0.23079793859009357 1104 6 0.1748472022061378 1104 7 0.09856868697541898 1104 8 0.21354696669283868 1104 9 0.3420948038810394 1104 10-11 0.25183286740575994 1104 12-13 0.31892536618483547 1104 14-15 0.30413315075102787 1104 16-17 0.3037683767270707 1104 18-19 0.42518722877542103 1104 20-21 0.49006414283628885 1104 22-23 0.287119993863584 1104 24-25 0.4106631028785799 1104 26-27 0.39760712358356187 1104 28-29 0.38693607018795007 1104 30-31 0.5524171113716179 1104 32-33 0.5075019773905751 1104 34-35 0.43315966636147607 1104 36-37 0.5545588488164981 1104 38-39 0.46647648158289456 1104 40-41 0.5795603312444229 1104 42-43 0.6090782681648506 1104 44-45 0.6949618710156429 1104 46-47 0.7512777267170705 1104 48-49 0.8351402508883226 1104 50-51 0.7694731529562162 1104 52-53 0.7209748760777188 1104 54-55 0.7460104248557116 1104 56-57 0.7576164746227576 1104 58-59 0.7576056000711233 1104 60-61 0.7972605458152984 1104 62-63 0.6624337289427018 1104 64-65 0.522247143533086 1104 66-67 0.4994289303167463 1104 68-69 0.7169956856822566 1104 70-71 0.6601055655489461 1104 72-73 0.5498634929265407 1104 74-75 0.5418559246835706 1104 76-77 0.51391534716576 1104 78-79 0.5715349923902657 1104 80-81 0.5198711069121842 1104 82-83 0.4686411536079831 1104 84-85 0.4197056829635635 1104 86-87 0.49485834521661687 1104 88-89 0.5050229542502116 1104 90-91 0.678915269169007 1104 92-93 0.5918533300299949 1104 94-95 0.5745565501180536 1104 96-97 0.5347884868762378 1104 98-99 0.4369687669487057 1104 100-101 0.9425777171825231 1105 1 0.12098923064841216 1105 2 0.1415623111730362 1105 3 0.10030405818197607 1105 4 0.11026267941095824 1105 5 0.15852849124060242 1105 6 0.17474684476468383 1105 7 0.10414136528102347 1105 8 0.11961487765583456 1105 9 0.20230475339644016 1105 10-11 0.1335397013251196 1105 12-13 0.19974587930412468 1105 14-15 0.30946338353950864 1105 16-17 0.3279318705060774 1105 18-19 0.2934262105770813 1105 20-21 0.2602997133588758 1105 22-23 0.24653138071894176 1105 24-25 0.27763756635225434 1105 26-27 0.20835134174227932 1105 28-29 0.20780714073529083 1105 30-31 0.23030825049412584 1105 32-33 0.19873038743156712 1105 34-35 0.136637219212993 1105 36-37 0.11760691842872717 1105 38-39 0.17335103915078065 1105 40-41 0.24635308802984213 1105 42-43 0.16535548775392073 1105 44-45 0.05743627185695743 1105 46-47 0.20670045148715133 1105 48-49 0.01296154764047941 1105 50-51 -0.13926275112012831 1105 52-53 -0.048409768214142446 1105 54-55 -6.163124641460627E-4 1105 56-57 -0.07774961883638554 1105 58-59 -0.12252297106613241 1105 60-61 -0.14686890800134478 1105 62-63 -0.2093290517012747 1105 64-65 -0.06864268759257897 1105 66-67 -0.19018888116274724 1105 68-69 -0.20461990890280646 1105 70-71 -0.33445576027627055 1105 72-73 -0.009983043212336895 1105 74-75 0.016149052893478455 1105 76-77 -0.08952258707316929 1105 78-79 -0.06159985946234059 1105 80-81 -0.11746244393509997 1105 82-83 -0.17539770176368918 1105 84-85 -0.16201112843727472 1105 86-87 0.035721666829442 1105 88-89 0.018254988228999736 1105 90-91 0.05238296106438156 1105 92-93 0.004173334807536122 1105 94-95 -0.11593148710013779 1105 96-97 -0.1445599471689647 1105 98-99 0.062366555297920456 1105 100-101 0.28526094598292673 1106 1 0.21121479455818815 1106 2 0.2655051469859693 1106 3 0.24292390567304523 1106 4 0.15206665744113934 1106 5 0.1854655146501898 1106 6 0.1296047341690425 1106 7 0.041007466704797935 1106 8 0.12368622624006065 1106 9 0.2294017512101263 1106 10-11 0.1665130922492395 1106 12-13 0.2300663613603362 1106 14-15 0.315877024023699 1106 16-17 0.4356919622247375 1106 18-19 0.3500838120275134 1106 20-21 0.4022196728320253 1106 22-23 0.342621339658848 1106 24-25 0.3402982073163727 1106 26-27 0.2528988808026966 1106 28-29 0.26609781422754253 1106 30-31 0.2105394135080445 1106 32-33 0.28564714741663977 1106 34-35 0.3109906559964415 1106 36-37 0.1281545641387538 1106 38-39 0.1559698201458204 1106 40-41 0.37313546951547494 1106 42-43 0.4717624626379404 1106 44-45 0.5378034131143536 1106 46-47 0.41592964148342304 1106 48-49 0.5142546740735767 1106 50-51 0.4613344866551543 1106 52-53 0.17469986757746625 1106 54-55 0.17246532780674073 1106 56-57 0.3484555216744596 1106 58-59 0.4316483615206579 1106 60-61 0.4455509714846073 1106 62-63 0.3817030498291416 1106 64-65 0.23232382443216437 1106 66-67 0.2894503765560188 1106 68-69 0.4015097770141125 1106 70-71 0.45598043659889953 1106 72-73 0.3040307145864176 1106 74-75 0.3791829674671483 1106 76-77 0.39639699912637383 1106 78-79 0.4523232461740925 1106 80-81 0.5343479330712455 1106 82-83 0.4342194261625103 1106 84-85 0.5449250951002931 1106 86-87 0.5289880775370612 1106 88-89 0.5150981599881845 1106 90-91 0.4763484599178973 1106 92-93 0.502929533815692 1106 94-95 0.6315969083565918 1106 96-97 0.7079423191350926 1106 98-99 0.49140857524139747 1106 100-101 0.9518796203283628 1107 1 0.016679318583928904 1107 2 -0.08185030889927702 1107 3 0.003302270140984831 1107 4 0.09121101799936326 1107 5 0.03873510304393335 1107 6 -0.015062731689582165 1107 7 0.002060299901899043 1107 8 0.19833408753349602 1107 9 0.11215505431754025 1107 10-11 0.04790015234012657 1107 12-13 0.05588500807127872 1107 14-15 -0.00465607677669766 1107 16-17 0.10276970590477674 1107 18-19 0.09089954507611253 1107 20-21 0.019799879753854555 1107 22-23 0.006501965470391724 1107 24-25 0.05985036579271963 1107 26-27 0.09838089800353345 1107 28-29 0.1513745645461384 1107 30-31 0.17402909611907091 1107 32-33 0.31025366298729296 1107 34-35 0.2839021369461818 1107 36-37 0.206653052142606 1107 38-39 0.18156587481458786 1107 40-41 0.28153096144556855 1107 42-43 0.1790796894810427 1107 44-45 0.31019856957423997 1107 46-47 0.3590605809932086 1107 48-49 0.32042563170660543 1107 50-51 0.3643401232826804 1107 52-53 0.23394235177074307 1107 54-55 0.2938049432257088 1107 56-57 0.3899591786637373 1107 58-59 0.3571380626133376 1107 60-61 0.33368656921382467 1107 62-63 0.2139494652963947 1107 64-65 0.40416766725176956 1107 66-67 0.40679697244350876 1107 68-69 0.3867337565812292 1107 70-71 0.4436559669036484 1107 72-73 0.3739739422746382 1107 74-75 0.540814570776714 1107 76-77 0.4858055494722464 1107 78-79 0.5510625166580034 1107 80-81 0.5357574399832501 1107 82-83 0.6478045273650217 1107 84-85 0.5777190578686984 1107 86-87 0.5386289255990242 1107 88-89 0.4462223184074112 1107 90-91 0.596423341460234 1107 92-93 0.6197220998183361 1107 94-95 0.5396094658072634 1107 96-97 0.539376591476973 1107 98-99 0.579836477289696 1107 100-101 0.8000619206626851 1108 1 0.12081444154149779 1108 2 0.07171028018091974 1108 3 0.0750146532672531 1108 4 0.09530474341242723 1108 5 0.19519580739684983 1108 6 0.19540004025340352 1108 7 0.16088249401749977 1108 8 0.15193481622176108 1108 9 0.2251837878728793 1108 10-11 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-1.7757039961683105 2316 82-83 -1.8329412712841062 2316 84-85 -1.8594703262983607 2316 86-87 -1.885234943306763 2316 88-89 -1.9676320356530574 2316 90-91 -2.230150788715232 2316 92-93 -2.3017739130985184 2316 94-95 -2.409659797189697 2316 96-97 -2.8189568142252988 2316 98-99 -3.1601009558664543 2316 100-101 -3.8225745376504676 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8662.0 3 2099.0 4 1924.0 5 2228.0 6 2613.0 7 3231.0 8 4136.0 9 4903.0 10 6300.0 11 8369.0 12 11507.0 13 17275.0 14 17215.0 15 16258.0 16 21714.0 17 29346.0 18 24767.0 19 16599.0 20 15578.0 21 16465.0 22 17979.0 23 19823.0 24 22549.0 25 25454.0 26 29818.0 27 35135.0 28 42066.0 29 49724.0 30 59566.0 31 72997.0 32 91067.0 33 116835.0 34 157819.0 35 224271.0 36 344812.0 37 551531.0 38 548336.0 39 90678.0 40 21.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.5424003360071 19.745483436083756 15.02108121444243 21.691035013466717 2 14.856631559816972 21.64787233730716 40.31955245361997 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29.882994730951644 21.05363743171822 22-23 21.63173984640922 26.892497083475813 29.97052505498606 21.505238015128906 24-25 21.89731014957909 26.9611304765223 30.133021710989215 21.008537662909397 26-27 21.61147360703812 26.68200146627566 30.769849706744868 20.93667521994135 28-29 20.866316270022285 27.67297176587923 30.45956525476209 21.0011467093364 30-31 21.310413428386813 27.05459808556499 30.152241454697926 21.482747031350275 32-33 21.216463028588738 27.243786086837652 30.154866579600448 21.384884304973166 34-35 21.941465341804367 27.028716068778913 29.947547580656277 21.082271008760443 36-37 21.765102852318563 26.642465399030907 30.415708789332047 21.176722959318486 38-39 21.487967857337477 27.195264023753523 30.292570544042253 21.024197574866747 40-41 21.467541430109407 27.2601194364953 30.09805202572762 21.174287107667674 42-43 21.1590797637263 27.419857799874237 29.432561708168702 21.988500728230758 44-45 21.571630817057347 27.173600635296946 29.325479395657762 21.929289151987945 46-47 22.017046579021446 27.576579722866523 28.9475006825517 21.458873015560336 48-49 21.87971460249059 27.473460119791532 28.968985200717196 21.677840077000685 50-51 21.30019469703749 27.769019418516137 29.32368649468367 21.6070993897627 52-53 21.707021606742664 28.21749298590589 28.424292988336454 21.651192419014993 54-55 21.19101827783921 27.567843640647155 28.853611214522406 22.387526866991227 56-57 21.842023549273147 27.79967196452608 28.3701540360493 21.988150450151476 58-59 22.305470667009246 27.350831948654108 28.498520595707777 21.84517678862887 60-61 21.881000340314376 27.484653661138857 29.26166496509478 21.372681033451986 62-63 21.627250993844918 27.492307528036974 29.1370440067939 21.74339747132421 64-65 21.32338980403972 28.004609833538087 28.81035466731834 21.861645695103856 66-67 21.505150571969594 27.65695711696897 28.331304562919236 22.5065877481422 68-69 21.254993504691118 27.626455918927345 28.78776520669412 22.330785369687415 70-71 21.850093903345766 27.617658212449392 28.376362620703606 22.155885263501244 72-73 21.778080868835932 27.122430330288633 28.917170566805243 22.182318234070195 74-75 21.25035680545401 28.292379740448204 28.273061602576732 22.184201851521053 76-77 21.552445886286122 27.457582990441754 28.373617685232805 22.616353438039322 78-79 21.603331751448458 27.561687067336383 28.510505053256995 22.324476127958164 80-81 21.782272622436054 27.71262797290405 28.323779753429832 22.181319651230062 82-83 21.651512853065324 27.517353184620507 28.66758618048785 22.163547781826324 84-85 21.535969138772234 27.589513413134025 28.383396108579174 22.491121339514564 86-87 21.520196119655672 27.552650270095324 28.491917759646647 22.43523585060236 88-89 21.67982749849433 27.434474634335583 28.0750030242264 22.810694842943683 90-91 21.65603441703586 27.15836631779924 28.427956733416398 22.757642531748505 92-93 22.02148299914823 27.562126199958563 28.313745712378278 22.10264508851493 94-95 21.79268921983501 27.290139106150807 28.25990791082208 22.657263763192102 96-97 21.353424471862695 27.208173223364042 28.626391819557647 22.812010485215616 98-99 21.678128239595587 27.391989845821723 27.935224386971136 22.994657527611555 100-101 21.86206185453119 26.704866206480048 28.517085762999596 22.915986175989158 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3661.0 1 2151.0 2 725.5 3 783.5 4 699.0 5 724.0 6 1138.0 7 2250.0 8 3685.0 9 4480.0 10 4298.0 11 4091.0 12 4321.0 13 4642.5 14 5054.0 15 5386.5 16 5763.0 17 6046.0 18 6032.5 19 6105.5 20 6516.5 21 7360.0 22 8422.5 23 9688.5 24 11250.0 25 13713.5 26 17520.0 27 21742.5 28 25246.0 29 29068.0 30 34405.5 31 40408.0 32 46373.5 33 52742.5 34 60304.5 35 66437.0 36 73063.0 37 80304.5 38 82870.0 39 82706.0 40 82301.0 41 84500.5 42 87781.0 43 90603.5 44 94875.5 45 97759.0 46 99617.5 47 111647.0 48 144354.0 49 143501.5 50 109198.5 51 92324.0 52 83147.5 53 78092.5 54 73268.5 55 66565.5 56 60498.5 57 53806.0 58 47611.0 59 41830.0 60 35717.5 61 28928.5 62 22339.0 63 16876.5 64 12227.0 65 8962.0 66 6337.5 67 4674.0 68 3428.5 69 2283.0 70 1652.0 71 1200.0 72 910.0 73 680.0 74 523.5 75 446.5 76 307.0 77 160.0 78 92.0 79 64.0 80 48.0 81 34.0 82 30.5 83 22.0 84 16.0 85 20.0 86 16.0 87 9.0 88 8.5 89 6.0 90 4.0 91 4.0 92 2.0 93 2.0 94 2.5 95 2.5 96 1.5 97 0.5 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2898593168281674 2 0.09071373921447319 3 0.09503344108182907 4 0.10865148425688316 5 0.11084794283350478 6 0.08533241570175021 7 0.09682721558607006 8 0.09298341307698221 9 0.09832812894676152 10-11 0.09086016978624797 12-13 0.09567407483334371 14-15 0.10495411231957008 16-17 0.09248920989724234 18-19 0.1042219594606962 20-21 0.10630859510848674 22-23 0.11901144720994848 24-25 0.1311102732028393 26-27 0.1343500496033562 28-29 0.12550930383245412 30-31 0.11864537078051156 32-33 0.11331895873220411 34-35 0.38923076359882414 36-37 0.5947826787276648 38-39 0.5775953903656006 40-41 0.5397796952047649 42-43 0.5673452503413663 44-45 0.5889986711425612 46-47 0.5768815413281985 48-49 0.5992488111667954 50-51 0.5919089787565848 52-53 0.5946728557988337 54-55 0.5848803113113956 56-57 0.5562714383508989 58-59 0.5504325193013798 60-61 0.49768090581951696 62-63 0.5748498171448235 64-65 0.6078516072585635 66-67 0.621286612218899 68-69 0.6230071714372527 70-71 0.6190535459993337 72-73 0.610652091943756 74-75 0.6078699110800353 76-77 0.5916344214345071 78-79 0.5674550732701973 80-81 0.5609572166476917 82-83 0.5762775152196276 84-85 0.5868022125659395 86-87 0.5775587827226568 88-89 0.5893647475719981 90-91 0.6041359314997785 92-93 0.6125373855553563 94-95 0.6110913836590803 96-97 0.6080163416518101 98-99 0.6123909549835814 100-101 0.5895660896081883 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2731670.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.57045440615115 #Duplication Level Percentage of deduplicated Percentage of total 1 72.36136390870037 34.42262962585742 2 14.54433988964546 13.837617151758897 3 5.4151872984210945 7.728087614409285 4 2.580019720602314 4.909308419435328 5 1.3841306470460102 3.292186191872936 6 0.8888361693961453 2.5369404282478425 7 0.5671989494729763 1.8887338235584732 8 0.37811219735920004 1.438957523590836 9 0.27908422308043096 1.1948546978571382 >10 1.398750645324619 12.143814542229928 >50 0.12326520081319021 4.060251385027485 >100 0.07322803510549963 6.243229817560679 >500 0.0033187374572874657 1.0217008063086996 >1k 0.0025469380486074383 2.574445182484245 >5k 3.8589970433446037E-4 1.239566717784271 >10k+ 2.315398226006762E-4 1.4676760720166528 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 16018 0.5863812246720871 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13549 0.4959969542441071 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10408 0.3810123477579649 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 9365 0.3428305761676923 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 7040 0.25771780632360425 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6466 0.236705019273924 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 5731 0.20979840171030908 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 5160 0.18889543758945992 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 4148 0.15184850293044183 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 4016 0.14701629406187422 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3951 0.14463679727053413 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3949 0.14456358198464678 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 3733 0.1366563311088089 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 3531 0.12926158723418274 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 3386 0.12395347900734716 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3205 0.11732749563453859 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 3.6607642943693786E-5 0.0 0.0 0.0 0.0 3 3.6607642943693786E-5 0.0 0.0 0.0 0.0 4 3.6607642943693786E-5 0.0 0.0 0.0 0.0 5 7.321528588738757E-5 0.0 0.0 0.0 0.0 6 7.321528588738757E-5 0.0 0.0 0.0 0.0 7 7.321528588738757E-5 0.0 0.0 0.0 0.0 8 7.321528588738757E-5 0.0 0.0 0.0 0.0 9 7.321528588738757E-5 0.0 0.0 3.6607642943693786E-5 3.6607642943693786E-5 10-11 7.321528588738757E-5 0.0 0.0 3.6607642943693786E-5 3.6607642943693786E-5 12-13 7.321528588738757E-5 0.0 0.0 5.491146441554068E-5 3.6607642943693786E-5 14-15 7.321528588738757E-5 0.0 0.0 1.2812675030292824E-4 3.6607642943693786E-5 16-17 7.321528588738757E-5 0.0 0.0 1.4643057177477514E-4 3.6607642943693786E-5 18-19 7.321528588738757E-5 0.0 0.0 1.6473439324662205E-4 3.6607642943693786E-5 20-21 7.321528588738757E-5 7.321528588738757E-5 0.0 2.562535006058565E-4 3.6607642943693786E-5 22-23 7.321528588738757E-5 7.321528588738757E-5 0.0 5.674184656272536E-4 3.6607642943693786E-5 24-25 7.321528588738757E-5 7.321528588738757E-5 0.0 0.001006710180951579 3.6607642943693786E-5 26-27 7.321528588738757E-5 7.321528588738757E-5 0.0 0.0019402050760157705 3.6607642943693786E-5 28-29 7.321528588738757E-5 7.321528588738757E-5 0.0 0.004813905047095732 3.6607642943693786E-5 30-31 7.321528588738757E-5 7.321528588738757E-5 0.0 0.013984119604491026 3.6607642943693786E-5 32-33 9.151910735923446E-5 7.321528588738757E-5 0.0 0.03212320668309129 3.6607642943693786E-5 34-35 1.2812675030292824E-4 7.321528588738757E-5 0.0 0.06162896689570849 3.6607642943693786E-5 36-37 1.4643057177477514E-4 7.321528588738757E-5 0.0 0.10726039382502278 3.6607642943693786E-5 38-39 1.4643057177477514E-4 7.321528588738757E-5 0.0 0.1755336479150117 3.6607642943693786E-5 40-41 1.4643057177477514E-4 7.321528588738757E-5 0.0 0.24808999622941277 7.321528588738757E-5 42-43 1.6473439324662205E-4 7.321528588738757E-5 0.0 0.3288281527417294 7.321528588738757E-5 44-45 2.562535006058565E-4 7.321528588738757E-5 0.0 0.42810808040502696 7.321528588738757E-5 46-47 2.562535006058565E-4 7.321528588738757E-5 0.0 0.5506155575160983 7.321528588738757E-5 48-49 2.562535006058565E-4 7.321528588738757E-5 0.0 0.6830070982219667 7.321528588738757E-5 50-51 2.562535006058565E-4 7.321528588738757E-5 0.0 0.8191692261510358 7.321528588738757E-5 52-53 2.562535006058565E-4 7.321528588738757E-5 0.0 0.9569054827266836 7.321528588738757E-5 54-55 2.562535006058565E-4 7.321528588738757E-5 0.0 1.1179791116789364 7.321528588738757E-5 56-57 2.928611435495503E-4 7.321528588738757E-5 0.0 1.3136286593915076 7.321528588738757E-5 58-59 2.928611435495503E-4 1.0982292883108135E-4 0.0 1.5228596426361896 7.321528588738757E-5 60-61 2.928611435495503E-4 1.0982292883108135E-4 0.0 1.7539636925397284 7.321528588738757E-5 62-63 2.928611435495503E-4 1.4643057177477514E-4 0.0 1.9909066614927864 7.321528588738757E-5 64-65 2.928611435495503E-4 1.4643057177477514E-4 0.0 2.242382864694491 7.321528588738757E-5 66-67 3.2946878649324404E-4 1.8303821471846892E-4 0.0 2.5337613987048213 7.321528588738757E-5 68-69 3.2946878649324404E-4 1.8303821471846892E-4 0.0 2.8635047425201434 7.321528588738757E-5 70-71 3.6607642943693784E-4 1.8303821471846892E-4 0.0 3.2247672669099856 7.321528588738757E-5 72-73 4.209878938524785E-4 1.8303821471846892E-4 0.0 3.591337899526663 7.321528588738757E-5 74-75 4.575955367961723E-4 1.8303821471846892E-4 0.0 3.979232484158042 7.321528588738757E-5 76-77 4.758993582680192E-4 1.8303821471846892E-4 0.0 4.393521179351826 7.321528588738757E-5 78-79 4.758993582680192E-4 1.8303821471846892E-4 0.0 4.872715225484777 7.321528588738757E-5 80-81 5.12507001211713E-4 1.8303821471846892E-4 0.0 5.388315572525231 7.321528588738757E-5 82-83 5.12507001211713E-4 1.8303821471846892E-4 0.0 5.918357634706974 1.0982292883108135E-4 84-85 5.12507001211713E-4 1.8303821471846892E-4 0.0 6.467252633004719 1.0982292883108135E-4 86-87 5.12507001211713E-4 1.8303821471846892E-4 0.0 7.040638144431794 1.0982292883108135E-4 88-89 5.12507001211713E-4 2.013420361903158E-4 0.0 7.66377344261935 1.2812675030292824E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTTGCGT 780 0.0 23.661804 3 GAGTACT 10470 0.0 23.549671 12-13 TAGATCG 105 0.002244764 22.535465 5 GTACATG 14295 0.0 22.192392 1 CTTTGCG 865 0.0 21.883753 2 AGTACTT 11050 0.0 21.585497 12-13 TACATGG 14645 0.0 21.359446 2 GTACTTT 11530 0.0 21.28282 14-15 TTGCGTT 860 0.0 20.910816 4 GTCTACG 230 9.1939E-8 20.61742 1 ACATGGG 14750 0.0 20.373217 3 GTATAGG 775 0.0 19.579897 1 CATGGGA 9030 0.0 18.39523 4 ACTTTTT 13820 0.0 18.35736 16-17 TACTTTT 13820 0.0 17.276781 14-15 CATGGGG 5225 0.0 17.2089 4 AGAGTAC 24025 0.0 16.9109 10-11 GTGTAGC 1495 0.0 16.811127 1 ATGGGAT 3320 0.0 15.964885 5 AGTACAT 11205 0.0 15.880124 2 >>END_MODULE