FastQCFastQC Report
Thu 26 May 2016
SRR938928_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938928_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2731670
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT173620.6355818967884115No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133910.49021294665900345No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108390.39679024186669687No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT88350.3234285254075346No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69330.25380078852862903No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT64920.23765681799046007No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC54590.19984112282962438No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT53110.1944231916739577No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC45510.16660138303675043No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC44630.16337991045770536No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT41260.15104313478568054No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA41070.1503475895697504No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA40380.1478216622066355No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG38860.14225730047919405No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA33620.12307489557669851No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC33460.12248917328959939No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA30050.11000596704579982No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACT117900.023.06177312-13
GTACATG135250.021.7722591
TTGCGTT7700.021.5677764
AGTACTT123050.021.38315612-13
TTTGCGT7350.021.303683
GTACTTT128550.021.07734514-15
TACATGG139450.020.8238472
ACATGGG139300.020.1650183
CATGGGG46250.018.8770484
CTTTGCG8050.018.8617552
ACTTTTT150150.018.09181616-17
TACTTTT149400.017.40531214-15
CATGGGA89400.017.1432464
GTGTAGC15450.016.9623681
AGAGTAC250950.016.84776910-11
TAGCCCT20300.015.8942814
ATCACGC1850.00316733615.3888993
AGTACAT107300.015.3004582
ATAGCCC9000.015.2891563
GTAAGCG3000.015.02527594-95
Produced by FastQC (version 0.11.5)