##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938928_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2731670 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.16267448117818 33.0 31.0 34.0 30.0 34.0 2 32.38831154568451 34.0 31.0 34.0 31.0 34.0 3 32.480519608883945 34.0 31.0 34.0 30.0 34.0 4 36.04935112952882 37.0 35.0 37.0 35.0 37.0 5 35.9712219997291 37.0 35.0 37.0 35.0 37.0 6 36.01898875047132 37.0 35.0 37.0 35.0 37.0 7 35.96506422810955 37.0 35.0 37.0 35.0 37.0 8 35.938074877272875 37.0 35.0 37.0 35.0 37.0 9 37.60729846577368 39.0 37.0 39.0 35.0 39.0 10-11 37.53270709858805 39.0 37.0 39.0 35.0 39.0 12-13 37.46685598919343 39.0 37.0 39.0 34.0 39.0 14-15 38.69332166769778 40.0 38.0 41.0 34.0 41.0 16-17 38.69630354325376 40.0 38.0 41.0 34.0 41.0 18-19 38.82990185490927 40.0 38.0 41.0 34.5 41.0 20-21 38.839737230338955 40.0 38.0 41.0 34.5 41.0 22-23 38.78253632393371 40.0 38.0 41.0 34.0 41.0 24-25 38.712846171023585 40.0 38.0 41.0 34.0 41.0 26-27 38.550702500668095 40.0 38.0 41.0 34.0 41.0 28-29 38.37363389428445 40.0 38.0 41.0 34.0 41.0 30-31 38.199350763452394 40.0 38.0 41.0 34.0 41.0 32-33 38.02211888698122 40.0 37.0 41.0 33.0 41.0 34-35 37.965112733236445 40.0 37.0 41.0 33.0 41.0 36-37 37.84727437794463 40.0 37.0 41.0 33.0 41.0 38-39 37.71065190890555 40.0 37.0 41.0 33.0 41.0 40-41 37.56237045470353 40.0 37.0 41.0 33.0 41.0 42-43 37.37516281249199 40.0 37.0 41.0 32.0 41.0 44-45 37.09028963967097 40.0 36.5 41.0 31.0 41.0 46-47 36.73247097929105 40.0 36.0 41.0 30.5 41.0 48-49 36.40049768090582 40.0 36.0 41.0 30.0 41.0 50-51 36.12672724011319 39.0 35.5 41.0 29.0 41.0 52-53 35.90753604937639 39.0 35.0 41.0 28.5 41.0 54-55 35.6756429217292 39.0 35.0 40.0 28.0 41.0 56-57 35.40491750467663 38.5 35.0 40.0 27.5 41.0 58-59 35.113380825648775 38.0 34.5 40.0 27.0 41.0 60-61 34.698213363986135 38.0 34.0 40.0 26.0 41.0 62-63 34.39947651070591 37.5 34.0 40.0 26.0 41.0 64-65 34.15503025621689 37.0 34.0 40.0 26.0 41.0 66-67 33.69715613525792 36.5 33.0 39.0 24.5 41.0 68-69 33.27914352758569 36.0 33.0 39.0 23.0 40.0 70-71 32.80735539065846 35.5 32.0 38.5 22.5 40.0 72-73 32.28718824016078 35.0 32.0 37.5 21.5 40.0 74-75 31.821573433101364 35.0 31.0 37.0 20.0 39.0 76-77 29.830367870203943 33.0 29.0 35.0 17.5 37.5 78-79 30.80928443040338 34.0 30.5 36.0 18.0 38.0 80-81 30.791755775770866 34.0 31.0 36.0 18.0 37.5 82-83 30.463143974198935 34.0 31.0 35.0 15.0 37.0 84-85 30.0583320825722 34.0 30.0 35.0 9.0 36.5 86-87 29.507932327111256 34.0 29.5 35.0 4.5 36.0 88-89 28.939575973671783 34.0 29.0 35.0 2.0 36.0 90-91 28.473765132684402 34.0 29.0 35.0 2.0 35.0 92-93 28.135377077026142 33.5 29.0 35.0 2.0 35.0 94-95 27.751241914286865 33.0 27.5 35.0 2.0 35.0 96-97 27.14225638528812 33.0 26.5 35.0 2.0 35.0 98-99 26.27458148312205 33.0 25.0 34.5 2.0 35.0 100-101 21.915178626993743 28.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.19271795551455284 1101 2 0.1592457254190549 1101 3 0.18302861455630648 1101 4 0.12933120424749234 1101 5 0.14986543178719813 1101 6 0.16200373077689534 1101 7 0.17573479957702176 1101 8 0.2595681999671342 1101 9 0.33734221839465306 1101 10-11 0.2862177138276891 1101 12-13 0.27973094286205935 1101 14-15 0.35271059129049576 1101 16-17 0.37025910940489837 1101 18-19 0.36977249951448954 1101 20-21 0.27551424600100205 1101 22-23 0.35057859362648003 1101 24-25 0.34205683085627925 1101 26-27 0.3675563056530393 1101 28-29 0.42369763483005585 1101 30-31 0.3211654997416531 1101 32-33 0.46618656059310126 1101 34-35 0.5841810603536786 1101 36-37 0.41573405012857023 1101 38-39 0.5608347508778948 1101 40-41 0.5381367578746321 1101 42-43 0.35891552761942336 1101 44-45 0.5655801686753463 1101 46-47 0.60404014155705 1101 48-49 0.544614970451498 1101 50-51 0.5871345790627416 1101 52-53 0.6269103880896765 1101 54-55 0.6096413570772938 1101 56-57 0.3725563634488296 1101 58-59 0.43563861663171366 1101 60-61 0.5448416477187763 1101 62-63 0.5825131724751884 1101 64-65 0.5660292575070329 1101 66-67 0.4534610317785166 1101 68-69 0.4324657890880417 1101 70-71 0.45527856679011336 1101 72-73 0.4889101518541068 1101 74-75 0.4645766824018587 1101 76-77 0.2438706407555742 1101 78-79 0.40549717642960204 1101 80-81 0.5537112417111523 1101 82-83 0.6086217603860362 1101 84-85 0.5673094301513153 1101 86-87 0.6350799079578557 1101 88-89 0.757267659311001 1101 90-91 0.7672262642645755 1101 92-93 0.6998607185912071 1101 94-95 0.7180356823851355 1101 96-97 0.6957560208138815 1101 98-99 0.7342417066823721 1101 100-101 1.2140161766174558 1102 1 0.1619827997867347 1102 2 0.10934525842809961 1102 3 0.10724136020938602 1102 4 0.09198470882557075 1102 5 0.06534379298283 1102 6 0.0791974111566418 1102 7 0.12137680641999538 1102 8 0.18083172007791148 1102 9 0.238953807394644 1102 10-11 0.26217460663057324 1102 12-13 0.22803657799562416 1102 14-15 0.23545903407542568 1102 16-17 0.26290247133582767 1102 18-19 0.17997876700140125 1102 20-21 0.2207079756107717 1102 22-23 0.2746229000164746 1102 24-25 0.16867736646631215 1102 26-27 0.1974458252753024 1102 28-29 0.23330201794315286 1102 30-31 0.08472687222421627 1102 32-33 0.147141294848808 1102 34-35 0.14495804897838838 1102 36-37 0.19337008120407262 1102 38-39 0.21166149969558035 1102 40-41 0.21165886310192406 1102 42-43 0.20344064408314466 1102 44-45 0.2088019044881051 1102 46-47 0.13857910554276742 1102 48-49 0.11800533802936286 1102 50-51 0.3230308873790122 1102 52-53 0.21183849016497902 1102 54-55 0.14174903639292324 1102 56-57 0.3002217589710554 1102 58-59 0.32598623424571116 1102 60-61 0.19167019280117614 1102 62-63 0.3120811348381949 1102 64-65 0.3393171887667634 1102 66-67 0.4784517380336979 1102 68-69 0.36585504267883806 1102 70-71 0.33957097040813977 1102 72-73 0.2939452156227631 1102 74-75 0.4331882032372043 1102 76-77 0.33024495366538886 1102 78-79 0.2975803890547013 1102 80-81 0.36946756319330376 1102 82-83 0.4073566434552589 1102 84-85 0.4303881117207915 1102 86-87 0.48673793412424615 1102 88-89 0.5104744746397145 1102 90-91 0.5768139217927626 1102 92-93 0.647595632586988 1102 94-95 0.6278478572224095 1102 96-97 0.3474364056159409 1102 98-99 0.7012467824240254 1102 100-101 1.1327308595981975 1103 1 0.014571683478571629 1103 2 0.08779329948356462 1103 3 0.10545448195379237 1103 4 0.05488197811869355 1103 5 0.02154015141417176 1103 6 -0.0511977401809105 1103 7 0.05239109581068391 1103 8 0.037853282837957636 1103 9 -0.04093472521435615 1103 10-11 0.1616391066897478 1103 12-13 0.04768913935998853 1103 14-15 0.09779896641354924 1103 16-17 0.004642914237436457 1103 18-19 0.05862601797960565 1103 20-21 0.09242091796293295 1103 22-23 0.15821870017163775 1103 24-25 0.0869153589262055 1103 26-27 0.10512386033850163 1103 28-29 0.1701867072376615 1103 30-31 0.10965276726003026 1103 32-33 0.1835597991119471 1103 34-35 0.2364593648345945 1103 36-37 0.13938663413042462 1103 38-39 0.154064848601287 1103 40-41 0.18563712564576207 1103 42-43 0.17951432076008444 1103 44-45 0.1672277516233791 1103 46-47 0.17930020601556862 1103 48-49 0.1366576702224762 1103 50-51 0.15537967662223906 1103 52-53 0.2136333518451039 1103 54-55 0.06279555064192266 1103 56-57 0.0710676544576927 1103 58-59 -0.008937444215753487 1103 60-61 -0.08259425203148396 1103 62-63 0.05522384277823278 1103 64-65 0.07354358553732965 1103 66-67 0.297714990048803 1103 68-69 0.2476533982812299 1103 70-71 0.17742198280028987 1103 72-73 0.25420701559383474 1103 74-75 0.21630884874873502 1103 76-77 0.15920125709563138 1103 78-79 0.19727617361870387 1103 80-81 0.2067296806229031 1103 82-83 0.21782310086228307 1103 84-85 0.11702179275574309 1103 86-87 0.11571274031487278 1103 88-89 0.3410608055141502 1103 90-91 0.47103425116068465 1103 92-93 0.5047904826207059 1103 94-95 0.48698737554953553 1103 96-97 0.4453643782205816 1103 98-99 0.4963289459508573 1103 100-101 1.1177598253408547 1104 1 0.07256845030244818 1104 2 0.06510762566026784 1104 3 0.11154229430488272 1104 4 0.06503130097760135 1104 5 0.1340644781698188 1104 6 0.08132240036873384 1104 7 0.04421018045665903 1104 8 0.10687224100707482 1104 9 0.06317265542310935 1104 10-11 0.08491352140025299 1104 12-13 0.1707725027538345 1104 14-15 0.16832317738052183 1104 16-17 0.18037004486042463 1104 18-19 0.14829321460214118 1104 20-21 0.11976708602574604 1104 22-23 0.10901964607605663 1104 24-25 0.16742856996888378 1104 26-27 0.18834029164241883 1104 28-29 0.0762464631016897 1104 30-31 0.1047874161308684 1104 32-33 0.12205151799486202 1104 34-35 0.07545298309714354 1104 36-37 0.014813604875968167 1104 38-39 0.26347178790575043 1104 40-41 0.2686130727748619 1104 42-43 0.24328048107395972 1104 44-45 0.2495134201025806 1104 46-47 0.20411238342163784 1104 48-49 0.2115551304423846 1104 50-51 0.12074995545027889 1104 52-53 0.1731629826128298 1104 54-55 0.19561629641007272 1104 56-57 0.16586963355337758 1104 58-59 0.18846888942486117 1104 60-61 0.31703032545117793 1104 62-63 0.24124280540075205 1104 64-65 0.20178622516288414 1104 66-67 0.31789562038454733 1104 68-69 0.47950240984673087 1104 70-71 0.26970110476585063 1104 72-73 0.200278439393756 1104 74-75 0.31431283009784394 1104 76-77 0.08803130158520389 1104 78-79 0.19188532003348513 1104 80-81 0.4679133081040092 1104 82-83 0.5151728761877123 1104 84-85 0.3149090947459712 1104 86-87 0.24082685896264167 1104 88-89 0.38731392846836954 1104 90-91 0.483018379205145 1104 92-93 0.34870511964546935 1104 94-95 0.4634266065345827 1104 96-97 0.33111880825082096 1104 98-99 0.39722497217254116 1104 100-101 1.1932941232829322 1105 1 -0.028930502426142368 1105 2 -0.03342517476388451 1105 3 -0.008587310994691677 1105 4 0.019892155166544967 1105 5 0.011255372880441428 1105 6 0.01739004914973208 1105 7 -0.02053959596036492 1105 8 0.012411855249965242 1105 9 -0.08154233818256529 1105 10-11 -0.03162921572151589 1105 12-13 -0.0406508396004952 1105 14-15 0.00914294765082957 1105 16-17 0.06845278075867611 1105 18-19 0.08856866591931123 1105 20-21 0.08994504933775005 1105 22-23 -0.06580993562713644 1105 24-25 0.07287744011014041 1105 26-27 0.022793554788663073 1105 28-29 0.1369031416053943 1105 30-31 0.21214256786141306 1105 32-33 0.0795499230543868 1105 34-35 0.12378841874166113 1105 36-37 0.11202631895484672 1105 38-39 0.11673639071459974 1105 40-41 0.1704961221262593 1105 42-43 0.1928668161483742 1105 44-45 0.15289644177919826 1105 46-47 0.32027772412713773 1105 48-49 0.2484707194924951 1105 50-51 0.2315919820588377 1105 52-53 0.32114760418225075 1105 54-55 0.3124488902930693 1105 56-57 0.3207707618099036 1105 58-59 0.25537605951979714 1105 60-61 0.24267507467410354 1105 62-63 0.21609205075357352 1105 64-65 0.2449767761237851 1105 66-67 0.4577895968988699 1105 68-69 0.2206383279061228 1105 70-71 0.21344493256471964 1105 72-73 0.1818613923204282 1105 74-75 0.1709654069098896 1105 76-77 4.218770164214902E-4 1105 78-79 0.2940567695794769 1105 80-81 0.23292716005274983 1105 82-83 0.36256635018654393 1105 84-85 0.2804412659333373 1105 86-87 0.1781899052843734 1105 88-89 0.35277305283876714 1105 90-91 0.4900098262458421 1105 92-93 0.4026527065515175 1105 94-95 0.3162165871039875 1105 96-97 0.4028352700756699 1105 98-99 0.6843288952891271 1105 100-101 1.447405282622583 1106 1 -0.07965038724456974 1106 2 -0.12862860888109395 1106 3 -0.06808445811781638 1106 4 2.7129450424467905E-4 1106 5 -0.06451650361931627 1106 6 -0.07964775813443481 1106 7 -0.04641012334264616 1106 8 -0.02023892062589283 1106 9 -0.05819940638498622 1106 10-11 0.005910111319494149 1106 12-13 0.048904164932110916 1106 14-15 0.08975804923458242 1106 16-17 0.04134645110475077 1106 18-19 -0.04261967727911298 1106 20-21 0.003617528407758641 1106 22-23 0.10860349155784377 1106 24-25 -0.02007568738945764 1106 26-27 0.04421546061706039 1106 28-29 0.016959665894837883 1106 30-31 0.06767901536314724 1106 32-33 0.04439021527430498 1106 34-35 0.03440272045338588 1106 36-37 0.0076430202826358595 1106 38-39 -0.01112048551853917 1106 40-41 0.03808530785654085 1106 42-43 0.06681498444145006 1106 44-45 -0.03430027341303088 1106 46-47 0.061096526452097066 1106 48-49 -0.1657363407111987 1106 50-51 -0.02850373595961031 1106 52-53 0.09445720797896939 1106 54-55 -0.0028593270588146424 1106 56-57 -0.017355138046120544 1106 58-59 0.03416212041672395 1106 60-61 0.12230233431255755 1106 62-63 0.14280111063733614 1106 64-65 0.1381592002692571 1106 66-67 0.24247338082677317 1106 68-69 0.08756895233954509 1106 70-71 0.014262135145635568 1106 72-73 0.13501955368128904 1106 74-75 0.23182500270789674 1106 76-77 0.07680698873187808 1106 78-79 0.12460654828154816 1106 80-81 0.3191746937806883 1106 82-83 0.4759722052497324 1106 84-85 0.3036042265263106 1106 86-87 0.36464407261759746 1106 88-89 0.315016427232198 1106 90-91 0.5593600635412024 1106 92-93 0.633855714070318 1106 94-95 0.684542988213142 1106 96-97 0.622036197927212 1106 98-99 0.733519691428409 1106 100-101 1.0858631293671053 1107 1 0.0029865933891670693 1107 2 -0.028633845352587173 1107 3 -0.10347334453314971 1107 4 -0.0703156411434378 1107 5 -0.10089681930293892 1107 6 -0.07387195808139779 1107 7 -0.12623397068185938 1107 8 0.007223152059133042 1107 9 -0.038520774520975465 1107 10-11 0.009955005521767646 1107 12-13 -0.01672366419762028 1107 14-15 0.062244521916582585 1107 16-17 0.03234098025215815 1107 18-19 -0.025523437933721027 1107 20-21 0.013887313099175458 1107 22-23 -0.07481176315169336 1107 24-25 -0.04373977810418239 1107 26-27 -0.09767401511133755 1107 28-29 -0.06968412551214698 1107 30-31 -0.08519045900432332 1107 32-33 0.010213698020692163 1107 34-35 -0.15594833875598368 1107 36-37 0.03398989255502016 1107 38-39 -0.010877843671067922 1107 40-41 -0.11050580128692644 1107 42-43 -0.10465474469295799 1107 44-45 -0.12494933545458053 1107 46-47 -0.26189897606294466 1107 48-49 -0.13793438742332853 1107 50-51 -0.10597817175780477 1107 52-53 0.03469793725424353 1107 54-55 -0.026532518592986776 1107 56-57 0.016687457149153317 1107 58-59 0.039484069605030925 1107 60-61 -0.16167347486929629 1107 62-63 -0.19690748672477554 1107 64-65 -0.26842760880703764 1107 66-67 -0.16569508066149297 1107 68-69 -0.07955429543768133 1107 70-71 -0.02643077884433609 1107 72-73 -0.10608103501572685 1107 74-75 -0.12753506407251436 1107 76-77 -0.396736999168791 1107 78-79 -0.19410295832242497 1107 80-81 -0.06075462505518203 1107 82-83 -0.025194029758068837 1107 84-85 -0.21050078348878998 1107 86-87 -0.21393975307006485 1107 88-89 -0.22903489914923014 1107 90-91 0.038500285326772854 1107 92-93 0.08713679749906689 1107 94-95 0.04075914191562191 1107 96-97 0.024472963333568742 1107 98-99 0.08578174910408265 1107 100-101 0.7926015440939622 1108 1 0.020023055248536537 1108 2 -0.011566220976980901 1108 3 -0.0704024815430131 1108 4 -0.02318562312063932 1108 5 0.03868923955933212 1108 6 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-1.1751544631359039 2316 60-61 -1.1672983227553573 2316 62-63 -1.2858000905524918 2316 64-65 -1.2928132688908036 2316 66-67 -1.339776279055485 2316 68-69 -1.3476458062940253 2316 70-71 -1.276049004861214 2316 72-73 -1.2760662071769744 2316 74-75 -1.4714259677868675 2316 76-77 -1.386726871970918 2316 78-79 -1.4703683180728717 2316 80-81 -1.6025852630352446 2316 82-83 -1.6692355499333011 2316 84-85 -1.761372138905564 2316 86-87 -1.8124664439591704 2316 88-89 -2.116253364495755 2316 90-91 -2.1693908459600557 2316 92-93 -2.565625625074521 2316 94-95 -2.6820771771374297 2316 96-97 -2.8785124112380345 2316 98-99 -3.2562432050418444 2316 100-101 -3.9174951697550213 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 14.0 4 15.0 5 65.0 6 139.0 7 375.0 8 860.0 9 1677.0 10 2711.0 11 4231.0 12 6489.0 13 8887.0 14 10492.0 15 14608.0 16 19537.0 17 28163.0 18 27042.0 19 17058.0 20 14676.0 21 15348.0 22 16215.0 23 18077.0 24 20927.0 25 24690.0 26 29540.0 27 35872.0 28 43260.0 29 52895.0 30 64601.0 31 78097.0 32 97304.0 33 124650.0 34 165889.0 35 238201.0 36 376346.0 37 621257.0 38 513067.0 39 38392.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.517251988762 20.39699148400738 15.481815063973789 21.60394146325684 2 15.131073665559896 22.178630654508048 39.51114153612991 23.179154143802144 3 19.43569318402296 26.243799580476413 31.038265969169043 23.282241266331585 4 12.624804606705789 18.103650880230774 38.41638997389875 30.855154539164687 5 12.753577773490596 38.6171836361593 34.41687591084825 14.212362679501855 6 29.354460824330904 37.731900266137565 17.84304107011462 15.070597839416914 7 26.46091951077546 34.43948207506764 21.582072505097614 17.51752590905929 8 25.434331379705455 34.18385090439182 20.36658893643815 20.01522877946458 9 25.833757371864095 17.705213294431612 21.736556758320003 34.72447257538429 10-11 24.295668949763332 27.149289628688678 27.60558559416035 20.94945582738764 12-13 26.220425600456863 25.026906617563615 27.808501758997245 20.944166022982277 14-15 22.71931378801113 26.12110553610037 27.64997067689156 23.50960999899694 16-17 20.806578562014934 29.41501317908918 28.065437838629375 21.71297042026651 18-19 20.697229167505593 28.37634121251835 30.40453275834929 20.52189686162677 20-21 22.194536675367083 26.765421884781105 29.93212942998239 21.10791200986942 22-23 21.540559437999466 26.96240761146112 29.983563168318284 21.513469782221133 24-25 21.882969026273305 27.068295218675754 30.155161494616845 20.893574260434093 26-27 21.531389223442073 26.77201126051097 30.798943503424645 20.897656012622313 28-29 20.815563373321083 27.80689102270772 30.454154418359465 20.92339118561173 30-31 21.28055365399188 27.054237883785376 30.196674561714993 21.468533900507747 32-33 21.184989402087368 27.268575633220703 30.201982669941827 21.3444522947501 34-35 21.946952837651974 27.048491968280942 29.963717328297957 21.040837865769124 36-37 21.7598518891944 26.690807527159844 30.439706699894213 21.109633883751542 38-39 21.39722866700765 27.233287482503886 30.40915000470468 20.96033384578378 40-41 21.422840875040748 27.31019950406328 30.122179375922194 21.144780244973777 42-43 21.141740916580545 27.458008026641746 29.49578515279942 21.904465903978288 44-45 21.598449879725106 27.16389563997556 29.320991382892224 21.916663097407106 46-47 21.97977713925703 27.632985937859782 28.96453842552205 21.42269849736113 48-49 21.878578981538883 27.544491711177137 28.957094045910903 21.619835261373073 50-51 21.25030155733543 27.8476438991336 29.253459352267658 21.64859519126331 52-53 21.75744141861938 28.308305908107496 28.281747063151847 21.65250561012128 54-55 21.197930203867962 27.614975454575408 28.77146580663111 22.41562853492552 56-57 21.83940593117031 27.898373522424013 28.256817258307187 22.00540328809849 58-59 22.30900483441241 27.41230077176346 28.40257557109837 21.876118822725765 60-61 21.832380261351794 27.651503850025193 29.07427518358709 21.441840705035922 62-63 21.590528870617607 27.596872975139753 28.973796249180907 21.83880190506174 64-65 21.28145016424061 28.07077593392743 28.68089877850965 21.966875123322314 66-67 21.48712770052513 27.71992752290169 28.213096140881888 22.57984863569129 68-69 21.16075314931819 27.77207908997691 28.619066393861818 22.448101366843083 70-71 21.699949115376306 27.680594654551978 28.256872169771608 22.362584060300108 72-73 21.575391610260393 27.21166905226473 28.784132051089628 22.42880728638525 74-75 21.024446583957797 28.332247306592674 28.11274787950228 22.530558229947246 76-77 21.279870830821 27.462177512320373 28.371896540032733 22.886055116825897 78-79 21.278032790966105 27.498758084349227 28.505066686386005 22.718142438298667 80-81 21.387246629351274 27.622480021378866 28.33900141671578 22.651271932554078 82-83 21.216984482020155 27.413651722206563 28.653991880424794 22.715371915348488 84-85 20.92337288179026 27.54968572338533 28.429898194144975 23.097043200679437 86-87 20.922000701340558 27.455356447264634 28.49803685135851 23.124606000036295 88-89 21.012604937738246 27.285108010151603 28.099019493394195 23.603267558715956 90-91 20.8847853957959 26.978869324570987 28.507302965207177 23.629042314425934 92-93 21.153781386477867 27.40272434078787 28.354687791717154 23.08880648101711 94-95 20.787046019467944 27.03941178839318 28.408793887987933 23.76474830415094 96-97 20.234549730667382 26.9991190336863 28.796671034585387 23.96966020106093 98-99 20.413519934691966 27.149857047154303 28.110954104998044 24.32566891315569 100-101 20.414081016933455 26.315393532182863 28.964457005776882 24.306068445106803 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1305.0 1 868.5 2 499.0 3 585.0 4 557.0 5 592.5 6 976.0 7 1916.0 8 3166.5 9 3792.5 10 3594.5 11 3377.5 12 3490.0 13 3741.5 14 4012.0 15 4268.0 16 4539.0 17 4789.0 18 5049.0 19 5447.5 20 6018.0 21 6920.0 22 8091.5 23 9567.0 24 11318.0 25 13641.5 26 17507.0 27 21875.5 28 25164.0 29 29324.5 30 34656.0 31 40371.0 32 46601.0 33 52898.0 34 60342.5 35 66625.5 36 73449.5 37 80625.5 38 82765.0 39 82517.5 40 82700.0 41 85439.5 42 88675.0 43 91474.0 44 95682.5 45 98481.0 46 100210.5 47 113434.0 48 146425.0 49 144984.0 50 111851.5 51 94500.5 52 83927.0 53 78442.5 54 73601.0 55 66686.0 56 60666.0 57 53983.5 58 47740.0 59 42138.5 60 35692.5 61 28785.0 62 22345.0 63 16605.5 64 11730.5 65 8584.0 66 5981.0 67 4380.5 68 3186.5 69 2031.5 70 1431.5 71 1032.5 72 770.5 73 590.0 74 444.0 75 357.5 76 247.0 77 113.5 78 48.0 79 23.5 80 17.5 81 12.5 82 3.5 83 4.0 84 4.5 85 2.0 86 2.0 87 2.0 88 3.0 89 3.0 90 1.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4121654519030483 2 0.0 3 0.0 4 0.0 5 4.758993582680192E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0013178751459729761 16-17 0.0025625350060585646 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0023062815054527086 36-37 0.007376440053154297 38-39 0.01277606738734913 40-41 0.0026174464704741053 42-43 6.223299300427943E-4 44-45 4.0268407238063165E-4 46-47 3.2946878649324404E-4 48-49 3.4777260796509097E-4 50-51 6.223299300427943E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 1.4643057177477514E-4 60-61 0.0023611929698682493 62-63 0.0 64-65 2.562535006058565E-4 66-67 0.0017754706827691486 68-69 0.001720559218353608 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.12684548279989896 78-79 1.0982292883108135E-4 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.14776675074221995 88-89 0.4357224701373153 90-91 0.3335505386814659 92-93 0.0 94-95 0.0 96-97 3.8438025090878477E-4 98-99 0.0 100-101 2.379496791340096E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2731670.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.73474822778118 #Duplication Level Percentage of deduplicated Percentage of total 1 70.5922886882649 32.28520549980641 2 15.398904373864344 14.085300290447284 3 5.7373587516731295 7.871899740006826 4 2.773918574010923 5.074578703470215 5 1.5120418373814517 3.457642637125618 6 0.9300976269801612 2.5522668478316652 7 0.6122139058470345 1.959961419182246 8 0.3929308231258092 1.4376473813274964 9 0.29117701823326786 1.19852168567531 >10 1.5362695523618821 12.695000306479137 >50 0.13609409801858907 4.3036518111555715 >100 0.07933818169065131 6.484518342220177 >500 0.004083641260263902 1.2033114302744028 >1k 0.0026423561095704996 2.6578025403910144 >5k 4.0035698629856055E-4 1.2095735275516377 >10k+ 2.4021419177913631E-4 1.5231178370550322 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 17362 0.6355818967884115 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13391 0.49021294665900345 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10839 0.39679024186669687 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 8835 0.3234285254075346 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6933 0.25380078852862903 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 6492 0.23765681799046007 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 5459 0.19984112282962438 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 5311 0.1944231916739577 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 4551 0.16660138303675043 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 4463 0.16337991045770536 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 4126 0.15104313478568054 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 4107 0.1503475895697504 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 4038 0.1478216622066355 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 3886 0.14225730047919405 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3362 0.12307489557669851 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 3346 0.12248917328959939 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 3005 0.11000596704579982 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 1.8303821471846893E-5 0.0 14-15 3.6607642943693786E-5 0.0 0.0 9.151910735923446E-5 0.0 16-17 7.321528588738757E-5 0.0 0.0 1.4643057177477514E-4 0.0 18-19 7.321528588738757E-5 0.0 0.0 2.745573220777034E-4 0.0 20-21 7.321528588738757E-5 0.0 0.0 3.6607642943693784E-4 0.0 22-23 7.321528588738757E-5 0.0 0.0 7.138490374020289E-4 0.0 24-25 1.0982292883108135E-4 0.0 0.0 0.001189748395670048 0.0 26-27 1.0982292883108135E-4 0.0 0.0 0.0021781547551497802 0.0 28-29 1.0982292883108135E-4 0.0 0.0 0.005033550904757895 0.0 30-31 1.0982292883108135E-4 3.6607642943693786E-5 0.0 0.014496626605702738 0.0 32-33 1.0982292883108135E-4 3.6607642943693786E-5 0.0 0.032855359541965175 0.0 34-35 1.4643057177477514E-4 3.6607642943693786E-5 0.0 0.06267228471960376 3.6607642943693786E-5 36-37 1.8303821471846892E-4 3.6607642943693786E-5 0.0 0.10865148425688315 3.6607642943693786E-5 38-39 1.8303821471846892E-4 3.6607642943693786E-5 0.0 0.1778399294204644 3.6607642943693786E-5 40-41 1.8303821471846892E-4 3.6607642943693786E-5 0.0 0.2513846840943452 3.6607642943693786E-5 42-43 2.196458576621627E-4 3.6607642943693786E-5 0.0 0.33309294314466975 3.6607642943693786E-5 44-45 2.562535006058565E-4 3.6607642943693786E-5 0.0 0.43370904977541214 3.6607642943693786E-5 46-47 3.294687864932441E-4 3.6607642943693786E-5 0.0 0.5571866294244913 3.6607642943693786E-5 48-49 3.6607642943693784E-4 3.6607642943693786E-5 0.0 0.6904750573824803 3.6607642943693786E-5 50-51 3.6607642943693784E-4 3.6607642943693786E-5 0.0 0.8276255916710291 3.6607642943693786E-5 52-53 3.6607642943693784E-4 5.491146441554068E-5 0.0 0.96618552021291 3.6607642943693786E-5 54-55 3.6607642943693784E-4 7.321528588738757E-5 0.0 1.1286868472399667 3.6607642943693786E-5 56-57 3.6607642943693784E-4 7.321528588738757E-5 0.0 1.3258556121347014 3.6607642943693786E-5 58-59 3.6607642943693784E-4 7.321528588738757E-5 0.0 1.5364593819897718 3.6607642943693786E-5 60-61 3.6607642943693784E-4 7.321528588738757E-5 0.0 1.768570142074262 3.6607642943693786E-5 62-63 3.6607642943693784E-4 7.321528588738757E-5 0.0 2.007215366424202 3.6607642943693786E-5 64-65 3.6607642943693784E-4 9.151910735923446E-5 0.0 2.2597531912712734 3.6607642943693786E-5 66-67 3.6607642943693784E-4 1.0982292883108135E-4 0.0 2.552358081320218 3.6607642943693786E-5 68-69 4.0268407238063165E-4 1.0982292883108135E-4 0.0 2.8831081353164913 3.6607642943693786E-5 70-71 4.0268407238063165E-4 1.0982292883108135E-4 0.0 3.2468416756050327 5.491146441554068E-5 72-73 4.0268407238063165E-4 1.0982292883108135E-4 0.0 3.615627070619804 1.4643057177477514E-4 74-75 4.0268407238063165E-4 1.0982292883108135E-4 0.0 4.006871254580531 1.4643057177477514E-4 76-77 4.0268407238063165E-4 1.0982292883108135E-4 0.0 4.424820714068684 1.4643057177477514E-4 78-79 4.0268407238063165E-4 1.0982292883108135E-4 0.0 4.905003166561114 1.4643057177477514E-4 80-81 4.758993582680192E-4 1.0982292883108135E-4 0.0 5.422342376641396 1.4643057177477514E-4 82-83 5.12507001211713E-4 1.0982292883108135E-4 0.0 5.953647402504695 1.4643057177477514E-4 84-85 5.12507001211713E-4 1.0982292883108135E-4 0.0 6.502670527552743 1.4643057177477514E-4 86-87 5.12507001211713E-4 1.0982292883108135E-4 0.0 7.075964519872459 1.4643057177477514E-4 88-89 5.491146441554068E-4 1.0982292883108135E-4 0.0 7.699209640988846 1.4643057177477514E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGTACT 11790 0.0 23.061773 12-13 GTACATG 13525 0.0 21.772259 1 TTGCGTT 770 0.0 21.567776 4 AGTACTT 12305 0.0 21.383156 12-13 TTTGCGT 735 0.0 21.30368 3 GTACTTT 12855 0.0 21.077345 14-15 TACATGG 13945 0.0 20.823847 2 ACATGGG 13930 0.0 20.165018 3 CATGGGG 4625 0.0 18.877048 4 CTTTGCG 805 0.0 18.861755 2 ACTTTTT 15015 0.0 18.091816 16-17 TACTTTT 14940 0.0 17.405312 14-15 CATGGGA 8940 0.0 17.143246 4 GTGTAGC 1545 0.0 16.962368 1 AGAGTAC 25095 0.0 16.847769 10-11 TAGCCCT 2030 0.0 15.894281 4 ATCACGC 185 0.003167336 15.388899 3 AGTACAT 10730 0.0 15.300458 2 ATAGCCC 900 0.0 15.289156 3 GTAAGCG 300 0.0 15.025275 94-95 >>END_MODULE