##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938927_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2250891 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.797103458141688 33.0 31.0 34.0 30.0 34.0 2 31.847870021249364 33.0 31.0 34.0 30.0 34.0 3 31.903395588680215 34.0 31.0 34.0 30.0 34.0 4 35.40811971792503 37.0 35.0 37.0 33.0 37.0 5 35.45950870122098 37.0 35.0 37.0 33.0 37.0 6 35.571579876591095 37.0 35.0 37.0 33.0 37.0 7 35.50688816117706 37.0 35.0 37.0 33.0 37.0 8 35.50642301204279 37.0 35.0 37.0 33.0 37.0 9 37.08134423212852 39.0 37.0 39.0 33.0 39.0 10-11 36.96670318553852 39.0 37.0 39.0 33.0 39.0 12-13 36.87624189709764 39.0 37.0 39.0 33.0 39.0 14-15 38.004972031075695 40.0 38.0 41.0 33.0 41.0 16-17 37.94799792615458 40.0 38.0 41.0 33.0 41.0 18-19 37.90557361507065 40.0 38.0 41.0 33.0 41.0 20-21 38.013764771372756 40.0 38.0 41.0 33.0 41.0 22-23 37.95345998540134 40.0 38.0 41.0 33.0 41.0 24-25 37.83537163727608 40.0 38.0 41.0 32.0 41.0 26-27 37.68958981132361 40.0 37.5 41.0 32.0 41.0 28-29 37.60006859505858 40.0 37.0 41.0 32.0 41.0 30-31 37.454276550930274 40.0 37.0 41.0 31.5 41.0 32-33 37.22669200774271 40.0 36.5 41.0 31.0 41.0 34-35 36.85530130068493 40.0 36.0 41.0 30.0 41.0 36-37 36.55526989090098 39.0 35.5 41.0 30.0 41.0 38-39 36.30053098972807 39.0 35.0 41.0 30.0 41.0 40-41 36.24384410440132 39.0 35.0 41.0 30.0 41.0 42-43 36.00709296896207 39.0 35.0 41.0 29.0 41.0 44-45 35.92378529213542 39.0 35.0 41.0 28.5 41.0 46-47 35.68017687218084 39.0 35.0 41.0 27.0 41.0 48-49 35.380721456525436 39.0 35.0 41.0 26.0 41.0 50-51 34.209849121969924 38.0 34.0 40.0 24.5 40.5 52-53 34.34533524724209 38.0 33.5 39.5 24.0 40.5 54-55 34.85980307353844 38.0 34.0 40.0 25.5 41.0 56-57 34.67434407085905 38.0 34.0 40.0 24.5 41.0 58-59 34.43443596335851 38.0 34.0 40.0 24.0 41.0 60-61 33.98541022199653 37.5 33.5 40.0 22.5 41.0 62-63 33.63263658702265 37.0 33.0 40.0 22.0 41.0 64-65 33.22965527873184 36.5 33.0 39.0 21.5 41.0 66-67 32.67507666963883 36.0 32.0 39.0 20.0 40.5 68-69 32.191816040847826 35.0 32.0 38.5 18.5 40.0 70-71 31.621902615453166 35.0 31.0 37.5 15.5 39.5 72-73 31.16534274649461 35.0 31.0 37.0 12.0 39.0 74-75 30.735618250728265 35.0 30.5 36.5 8.0 39.0 76-77 30.328808458517095 34.0 30.0 36.0 7.0 38.0 78-79 29.91043080273545 34.0 30.0 35.5 2.0 37.0 80-81 29.413281451656253 34.0 29.0 35.0 2.0 37.0 82-83 28.939103670502035 34.0 29.0 35.0 2.0 36.0 84-85 28.514913649750255 34.0 29.0 35.0 2.0 36.0 86-87 28.197205684326782 34.0 28.0 35.0 2.0 35.5 88-89 27.67358925865357 34.0 26.5 35.0 2.0 35.0 90-91 27.171516301766722 33.0 25.5 35.0 2.0 35.0 92-93 26.64167256433119 33.0 25.0 35.0 2.0 35.0 94-95 26.054410897728943 33.0 24.0 35.0 2.0 35.0 96-97 25.34450157737536 32.0 19.5 35.0 2.0 35.0 98-99 24.496237712088238 32.0 9.5 34.0 2.0 35.0 100-101 21.219425329791626 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.03700625290369075 1101 2 0.276764252078344 1101 3 0.318113082066251 1101 4 0.21324160132439118 1101 5 0.21138310114630343 1101 6 0.2583378756683459 1101 7 0.2767849731662011 1101 8 0.1977389027257317 1101 9 0.3772099078475719 1101 10-11 0.2515065862899135 1101 12-13 0.22056233348967424 1101 14-15 0.3095908520800279 1101 16-17 0.2423703006449216 1101 18-19 0.3977649189260788 1101 20-21 0.3819492961290507 1101 22-23 0.48502959050653516 1101 24-25 0.5226857405254535 1101 26-27 0.6150830881118594 1101 28-29 0.4910905638736338 1101 30-31 0.44192767805322575 1101 32-33 0.4014653409370865 1101 34-35 0.31788076998324755 1101 36-37 0.16566005124968086 1101 38-39 0.4599724318507228 1101 40-41 0.40566874219429394 1101 42-43 0.3143688315138178 1101 44-45 0.3015577337916042 1101 46-47 0.3764296313974995 1101 48-49 0.4709606895698286 1101 50-51 0.3235412233642947 1101 52-53 0.4060364089754458 1101 54-55 0.43982248936197266 1101 56-57 0.4611084972614421 1101 58-59 0.40866667639971155 1101 60-61 0.5454313159248159 1101 62-63 0.49405332125946444 1101 64-65 0.45326718373591035 1101 66-67 0.5957333443842003 1101 68-69 0.3914913901758368 1101 70-71 0.29710621345706656 1101 72-73 0.3021034910363234 1101 74-75 0.11262953131596021 1101 76-77 0.08245155560316775 1101 78-79 0.05052456354071211 1101 80-81 0.14616505177100692 1101 82-83 0.14526495356298597 1101 84-85 -0.0019989138007829865 1101 86-87 -0.11035132519031876 1101 88-89 -0.17969408687338628 1101 90-91 -0.2923941195779882 1101 92-93 -0.21987798220451893 1101 94-95 -0.46973033661448227 1101 96-97 -0.47077896531193275 1101 98-99 -0.5457350121636324 1101 100-101 -0.19932785588104096 1102 1 0.18952530711515436 1102 2 0.203915852148306 1102 3 0.30868901607615484 1102 4 0.15026129191674187 1102 5 0.19064350374095085 1102 6 0.23621503368804753 1102 7 0.27513028981221055 1102 8 0.28378243713309814 1102 9 0.2349997123558225 1102 10-11 0.37759897037369683 1102 12-13 0.30756706820071145 1102 14-15 0.4441467996362931 1102 16-17 0.5022325762834399 1102 18-19 0.45593643567108444 1102 20-21 0.3895666777007989 1102 22-23 0.43369974982505255 1102 24-25 0.3997053659911174 1102 26-27 0.5184953084857256 1102 28-29 0.49107765483143595 1102 30-31 0.508721853493391 1102 32-33 0.3900328140583653 1102 34-35 0.4406722770894973 1102 36-37 0.4489171165661716 1102 38-39 0.485134364736723 1102 40-41 0.4906012848895003 1102 42-43 0.31229652320030254 1102 44-45 0.48361721214534725 1102 46-47 0.5798361299256314 1102 48-49 0.5107028622709535 1102 50-51 0.4426066691844639 1102 52-53 0.509171050052899 1102 54-55 0.6312476522717603 1102 56-57 0.5370101764138226 1102 58-59 0.5400195533251377 1102 60-61 0.47661960837719164 1102 62-63 0.4476768312536237 1102 64-65 0.3588173043133409 1102 66-67 0.43122876783799313 1102 68-69 0.47054752752990936 1102 70-71 0.4339399673228037 1102 72-73 0.37853432462085834 1102 74-75 0.3543669302300181 1102 76-77 0.3154199933439017 1102 78-79 0.25828071150113274 1102 80-81 0.2665461732155521 1102 82-83 0.32589048155443834 1102 84-85 0.30202426479151256 1102 86-87 0.016627130897543196 1102 88-89 0.11892311048283943 1102 90-91 0.1750793971149136 1102 92-93 0.2784491920038228 1102 94-95 0.29790399650672583 1102 96-97 0.14599623034727927 1102 98-99 -0.010933862584352028 1102 100-101 -0.1488051210806809 1103 1 0.2627731410542964 1103 2 0.2819082166409075 1103 3 0.3325067697738895 1103 4 0.29843539977400013 1103 5 0.07756804023821928 1103 6 0.2252564803195085 1103 7 0.19046714370883677 1103 8 0.14626603450867748 1103 9 0.3331733629970728 1103 10-11 0.33329645339954794 1103 12-13 0.3428007055826967 1103 14-15 0.41805153868024547 1103 16-17 0.4264317624389449 1103 18-19 0.42991192889285657 1103 20-21 0.3691350657857484 1103 22-23 0.3965052272728684 1103 24-25 0.4629099930171563 1103 26-27 0.506425834512747 1103 28-29 0.39404875877949763 1103 30-31 0.49009212988047324 1103 32-33 0.5356181648573468 1103 34-35 0.36549704905301184 1103 36-37 0.4966470390680584 1103 38-39 0.589692808373421 1103 40-41 0.4691003590270739 1103 42-43 0.39940897547838006 1103 44-45 0.5772789408790899 1103 46-47 0.605516010135041 1103 48-49 0.7170681092819038 1103 50-51 0.6419617659999446 1103 52-53 0.5578248918104336 1103 54-55 0.5696743719754807 1103 56-57 0.7449484751130413 1103 58-59 0.7534258126123419 1103 60-61 0.7866079493688929 1103 62-63 0.7186352703181598 1103 64-65 0.6846805281434385 1103 66-67 0.8004108083930532 1103 68-69 0.773227270574516 1103 70-71 0.8106748734792184 1103 72-73 0.650885329353045 1103 74-75 0.716612089455495 1103 76-77 0.7786254204979599 1103 78-79 0.5026854162538932 1103 80-81 0.6289003784598215 1103 82-83 0.9127774120347425 1103 84-85 0.7066624926222502 1103 86-87 0.7340562052637232 1103 88-89 0.6318023140125497 1103 90-91 0.5901598671329751 1103 92-93 0.6915395150269354 1103 94-95 0.3784742591773238 1103 96-97 0.280818487622728 1103 98-99 0.47378049171233627 1103 100-101 0.899296175126711 1104 1 0.142978768759928 1104 2 0.14753592226861656 1104 3 0.19893804683016825 1104 4 0.21712858159218484 1104 5 0.2079035380737153 1104 6 0.24809738941041815 1104 7 0.24647093158762345 1104 8 0.17747815572079872 1104 9 0.28000236732607675 1104 10-11 0.2633492131398114 1104 12-13 0.21548062982512306 1104 14-15 0.4762685300222387 1104 16-17 0.36494231438699387 1104 18-19 0.4441719397153676 1104 20-21 0.34783636448704414 1104 22-23 0.3533842857144265 1104 24-25 0.34092812072278633 1104 26-27 0.25575213321404533 1104 28-29 0.38603198388772597 1104 30-31 0.3743900194908534 1104 32-33 0.4370372665889519 1104 34-35 0.41983417026514047 1104 36-37 0.3573883810594012 1104 38-39 0.6310821482002638 1104 40-41 0.5455559867327011 1104 42-43 0.5443778607597665 1104 44-45 0.6972424149050696 1104 46-47 0.7587411183601489 1104 48-49 0.6010589101477066 1104 50-51 0.4472363872120724 1104 52-53 0.5959864177844665 1104 54-55 0.8017631165642314 1104 56-57 0.7449241244636937 1104 58-59 0.6491143948634246 1104 60-61 0.7135789991524462 1104 62-63 0.593798960794345 1104 64-65 0.6612646731650855 1104 66-67 0.6167703863151317 1104 68-69 0.6208287316134786 1104 70-71 0.629646734951077 1104 72-73 0.5562492362794558 1104 74-75 0.5700874682433756 1104 76-77 0.5876378663854069 1104 78-79 0.4586134465569245 1104 80-81 0.476101406598346 1104 82-83 0.55413563714297 1104 84-85 0.5507561073408667 1104 86-87 0.7050721684671863 1104 88-89 0.6015669244021602 1104 90-91 0.4413422264403337 1104 92-93 0.4336742552866717 1104 94-95 0.46854595831152324 1104 96-97 0.35859581446255717 1104 98-99 0.5023126886820322 1104 100-101 0.5091541296721651 1105 1 0.1400025782837382 1105 2 0.15928607687591523 1105 3 0.23164715319999019 1105 4 0.23364257355260065 1105 5 0.19379562218009028 1105 6 0.19578214513089165 1105 7 0.177158547543101 1105 8 0.29436127260391487 1105 9 0.22616037598408667 1105 10-11 0.22086602668341015 1105 12-13 0.33397262889747736 1105 14-15 0.30539847745576054 1105 16-17 0.34168841055272736 1105 18-19 0.2731617742855619 1105 20-21 0.34002869596509555 1105 22-23 0.2643932915894993 1105 24-25 0.3377924914710846 1105 26-27 0.25612898850161514 1105 28-29 0.27947856706644814 1105 30-31 0.3466041892496676 1105 32-33 0.40840109621171194 1105 34-35 0.48093304163186446 1105 36-37 0.5452555733908824 1105 38-39 0.5793805016325422 1105 40-41 0.5518369081921932 1105 42-43 0.5205345166039237 1105 44-45 0.5547959476818107 1105 46-47 0.5970991888610726 1105 48-49 0.48234949456328735 1105 50-51 0.4762784017451551 1105 52-53 0.47794857764841225 1105 54-55 0.5974109211406073 1105 56-57 0.6132663477166318 1105 58-59 0.5799131348139426 1105 60-61 0.7597341089236238 1105 62-63 0.8138486283886621 1105 64-65 0.8076149746493186 1105 66-67 0.7994244138352329 1105 68-69 0.6861648092263337 1105 70-71 0.6853440138626432 1105 72-73 0.7056626948446976 1105 74-75 0.7549639756645234 1105 76-77 0.67254626156166 1105 78-79 0.6538728002984229 1105 80-81 0.7081428027022447 1105 82-83 0.6875424089425977 1105 84-85 0.543035405423737 1105 86-87 0.650722871930391 1105 88-89 0.9094770202586808 1105 90-91 0.7106391497551137 1105 92-93 0.8877268990714597 1105 94-95 0.8935855764314979 1105 96-97 0.671158623677151 1105 98-99 0.5592999722318197 1105 100-101 0.609064264180514 1106 1 0.16262226454391282 1106 2 0.1900330680340332 1106 3 0.11571883989472198 1106 4 0.16822810668881516 1106 5 0.17571688488729365 1106 6 0.209018017617133 1106 7 0.1760110989214283 1106 8 0.1193239857872399 1106 9 0.23037393364748482 1106 10-11 0.20642238556997228 1106 12-13 0.25274547786291635 1106 14-15 0.19558185448337895 1106 16-17 0.23657064232135383 1106 18-19 0.253507242642506 1106 20-21 0.2954766346420712 1106 22-23 0.3911769952083688 1106 24-25 0.38396656090077386 1106 26-27 0.23107955346741704 1106 28-29 0.2305430414676053 1106 30-31 0.2781888810639188 1106 32-33 0.2499700227135193 1106 34-35 0.32064457515378564 1106 36-37 0.2980250301192271 1106 38-39 0.4223517366746492 1106 40-41 0.43121079285108266 1106 42-43 0.2856039909334953 1106 44-45 0.4633575566195347 1106 46-47 0.2705741559535184 1106 48-49 0.21276802065816725 1106 50-51 0.17402864449896782 1106 52-53 0.14844017832449907 1106 54-55 0.09825876987445525 1106 56-57 0.16476247334056637 1106 58-59 0.28902164513508666 1106 60-61 0.5416796049035497 1106 62-63 0.34961607571339925 1106 64-65 0.2714640268357016 1106 66-67 0.2840731303349955 1106 68-69 0.34465757124676344 1106 70-71 0.2852308901664209 1106 72-73 0.22259598365561928 1106 74-75 0.3327461490711734 1106 76-77 0.18125181740261453 1106 78-79 0.33841169859615405 1106 80-81 0.4596320646885559 1106 82-83 0.5278478787143825 1106 84-85 0.5976728032376464 1106 86-87 0.5869624154100599 1106 88-89 0.7591746201751413 1106 90-91 0.905347706226852 1106 92-93 1.0342170326976436 1106 94-95 0.7733841223509188 1106 96-97 0.9253134403927596 1106 98-99 1.1262725479992177 1106 100-101 1.122630265004151 1107 1 0.19563028391144144 1107 2 0.20220422693692086 1107 3 0.2649520722191916 1107 4 0.2825795439181462 1107 5 0.23823728090746243 1107 6 0.18489611995914856 1107 7 0.1313680446097365 1107 8 0.18869846694111203 1107 9 0.23678983661354636 1107 10-11 0.24713171723481508 1107 12-13 0.31307097585296617 1107 14-15 0.3921699427986596 1107 16-17 0.27726187826905146 1107 18-19 0.3094936534745827 1107 20-21 0.2624555291062123 1107 22-23 0.2923546481222914 1107 24-25 0.2555869556941204 1107 26-27 0.24911566720258094 1107 28-29 0.344808089538823 1107 30-31 0.36661079139913255 1107 32-33 0.2961908804300677 1107 34-35 0.34392047247644086 1107 36-37 0.3044655718865883 1107 38-39 0.45822562690624125 1107 40-41 0.3007412856680034 1107 42-43 0.41978864085804446 1107 44-45 0.34693145053159924 1107 46-47 0.5166035262225606 1107 48-49 0.5618364490502401 1107 50-51 0.5063311353693152 1107 52-53 0.4868660758516228 1107 54-55 0.5796808069819193 1107 56-57 0.5440411392057101 1107 58-59 0.5099316041181297 1107 60-61 0.4661703689313157 1107 62-63 0.4319042535871489 1107 64-65 0.5465981600610732 1107 66-67 0.4069487749310241 1107 68-69 0.5528862152334568 1107 70-71 0.5549026727070192 1107 72-73 0.5766897051574205 1107 74-75 0.38503551287891824 1107 76-77 0.4145906714632126 1107 78-79 0.5032259567944308 1107 80-81 0.5049750245344669 1107 82-83 0.534843049100381 1107 84-85 0.6822287731885339 1107 86-87 0.7869197831273027 1107 88-89 0.8105989678092023 1107 90-91 0.657714564687673 1107 92-93 0.709280827768243 1107 94-95 0.699787000490069 1107 96-97 0.8065649483691892 1107 98-99 0.7558075187393634 1107 100-101 1.096683562514098 1108 1 0.2800072605085333 1108 2 0.1281149574017313 1108 3 0.2295348713370977 1108 4 0.19985222456843132 1108 5 0.20494583006382783 1108 6 0.22596489271672482 1108 7 0.26977770664368705 1108 8 0.27796116417344763 1108 9 0.41404080674876553 1108 10-11 0.2953525661672316 1108 12-13 0.3926425778154581 1108 14-15 0.4929105790706032 1108 16-17 0.3760314732910288 1108 18-19 0.4638673820418546 1108 20-21 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2316 100-101 -2.662040821210283 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6512.0 3 1855.0 4 1911.0 5 2225.0 6 2558.0 7 3101.0 8 3935.0 9 4924.0 10 6243.0 11 7896.0 12 10205.0 13 13962.0 14 12860.0 15 12754.0 16 15843.0 17 20752.0 18 18816.0 19 14861.0 20 15217.0 21 16542.0 22 18027.0 23 20257.0 24 22831.0 25 26290.0 26 29973.0 27 34841.0 28 40735.0 29 48801.0 30 58574.0 31 71310.0 32 88856.0 33 113507.0 34 152405.0 35 217969.0 36 325147.0 37 442916.0 38 309527.0 39 35949.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.63579207194856 18.36118065450973 15.024360370350726 20.978666903190984 2 15.494963678815527 22.183112839117406 36.14403616244465 26.17788731962242 3 22.81427933608807 23.46304495155492 30.113459496285017 23.60921621607199 4 12.862522669479654 16.315265643163933 38.660590638338746 32.16162104901766 5 13.422892223774468 38.05679090427526 33.65419042294515 14.866126449005115 6 30.63757510669518 35.72916115650864 16.221268091284223 17.411995645511958 7 26.93651696106807 33.269618104252636 20.949345059306285 18.84451987537301 8 26.227505621094238 29.701648587591716 19.748822215169 24.322023576145046 9 24.396377509333178 18.989669713563266 22.596512472538365 34.01744030456519 10-11 26.057652214346106 24.643040988496406 26.188811313155743 23.110495484001746 12-13 28.904875448857975 23.08363538730031 25.94423537005278 22.067253793788936 14-15 24.04219270675784 24.82661041446584 27.67663400722919 23.45456287154713 16-17 21.64366334859973 27.94039659442133 27.33157806066226 23.084361996316684 18-19 21.43168811261775 28.815965221171997 27.669689156889493 22.082657509320757 20-21 23.92622436107607 25.121594211757646 26.569721757589566 24.38245966957672 22-23 22.60683399657633 25.15662168471132 28.155043240479316 24.081501078233032 24-25 24.913467498730473 25.408761571387533 27.0125031904969 22.665267739385094 26-27 23.410301558928936 23.880470257627902 30.55980933578778 22.14941884765538 28-29 21.76979865368955 28.27712145939893 27.745360177729523 22.207719709182 30-31 22.75713047151146 25.21861592157037 27.22690794300069 24.79734566391748 32-33 23.199126310234554 25.516924960576816 28.30580119867817 22.978147530510455 34-35 25.04924896443467 25.01285821379132 27.29633881861317 22.641554003160845 36-37 23.64773682395213 24.266765786310103 29.927926178189885 22.157571211547886 38-39 22.861344489051845 26.159383221606525 28.587376790284814 22.391895499056815 40-41 22.730773015602765 26.82159274823835 27.73408779169416 22.713546444464725 42-43 22.314445463029493 26.685291371498355 25.900214527850117 25.100048637622034 44-45 23.387751348098195 24.527507096546064 27.02920142761344 25.055540127742294 46-47 25.22155016025988 26.338938429803626 27.15641002689562 21.28310138304087 48-49 23.96131803558312 25.71044564855808 27.36107111042645 22.96716520543235 50-51 20.91191826906226 26.608700544261215 29.73357566378369 22.745805522892837 52-53 23.213718024050163 27.90996101180005 25.397293865296934 23.47902709885286 54-55 22.16004368098948 25.501657234596912 27.111411524566538 25.22688755984706 56-57 24.15429632620249 24.898224085333535 26.570426745779713 24.377052842684265 58-59 26.07779602837215 25.10397892461726 26.146345469098414 22.671879577912176 60-61 23.573856809203477 25.876130844877938 29.03590532163739 21.5141070242812 62-63 22.004018205807046 26.451186790321398 28.37332752395087 23.17146747992069 64-65 21.9100976843803 28.456690385412692 26.642564652020745 22.990647278186263 66-67 23.280062716321403 25.777662959612858 25.54761231185159 25.39466201221414 68-69 22.19204249765743 25.85540649059354 27.278389312342956 24.67416169940607 70-71 24.906534814246456 25.735138131071437 25.80628377240719 23.55204328227491 72-73 23.380783347228803 24.380587307236645 29.12997987352518 23.108649472009375 74-75 21.930416461616918 28.73153324217365 26.304853226061432 23.033197070148002 76-77 22.878620458730957 25.708443551537474 26.121948769462584 25.29098722026899 78-79 23.180650020923423 25.93934997907658 27.19044776119403 23.68955223880597 80-81 24.79893901311882 25.61713274187749 26.133096090333872 23.45083215466982 82-83 23.416088093171762 25.000714288265314 28.594253908049673 22.98894371051325 84-85 22.842458865738834 26.511422233918903 27.276237900686688 23.369880999655575 86-87 22.667535432568187 27.055093419120617 26.79131626962815 23.486054878683046 88-89 22.399818378221994 26.88587183029704 25.176961804288243 25.537347987192717 90-91 23.30923423634619 24.912670355878067 26.26993578485941 25.50815962291633 92-93 24.68915832556885 26.336144176682346 26.61577720633564 22.358920291413163 94-95 23.67993093994513 25.6156578401182 26.924891608002017 23.779519611934653 96-97 21.140899213022823 26.052545970635325 29.068521515777835 23.738033300564016 98-99 23.016379650462962 27.22075564001521 25.502476518124702 24.260388191397126 100-101 22.75339490960004 24.59907564165756 27.11849234582717 25.529037102915233 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1961.0 1 1174.5 2 429.5 3 468.5 4 431.5 5 465.5 6 797.0 7 1556.5 8 2609.0 9 3178.0 10 2930.0 11 2642.5 12 2661.0 13 2864.0 14 3103.5 15 3451.0 16 3978.0 17 4140.0 18 3923.0 19 3921.5 20 4222.5 21 4615.5 22 5127.0 23 5846.0 24 6689.5 25 7877.0 26 9369.5 27 11124.5 28 12856.0 29 14758.0 30 16987.0 31 19602.0 32 22313.5 33 24538.0 34 27164.5 35 29998.0 36 33627.0 37 37120.0 38 39549.0 39 41615.5 40 43155.0 41 44908.5 42 46867.5 43 50376.0 44 55083.0 45 60315.5 46 71963.0 47 120747.0 48 204668.5 49 201151.5 50 121854.5 51 83108.0 52 75756.0 53 75306.0 54 75076.5 55 72329.5 56 67200.0 57 61233.0 58 56162.0 59 50635.5 60 43521.5 61 36328.0 62 29395.5 63 22839.5 64 16933.0 65 12284.0 66 8890.0 67 6731.5 68 4911.0 69 3423.0 70 2435.0 71 1562.0 72 1020.0 73 663.0 74 405.0 75 262.0 76 193.5 77 125.0 78 81.0 79 62.0 80 45.5 81 34.5 82 27.0 83 20.5 84 19.0 85 13.5 86 8.0 87 8.0 88 10.0 89 7.0 90 4.0 91 7.0 92 5.5 93 3.0 94 2.0 95 2.0 96 3.5 97 2.0 98 1.0 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3237828930854493 2 0.0856549695209586 3 0.09591757219696556 4 0.10098223325785212 5 0.11275534888184278 6 0.05588897907539725 7 0.05766605313184868 8 0.05904328552559853 9 0.06170889661027566 10-11 0.059220992931243666 12-13 0.05744391887479225 14-15 0.05600004620392547 16-17 0.05722178461773582 18-19 0.06559624610876316 20-21 0.06679577109686786 22-23 0.08178983344817674 24-25 0.0899643741078533 26-27 0.08489971304696674 28-29 0.08243402279364039 30-31 0.0773471483070482 32-33 0.0701944252298312 34-35 0.319384634795732 36-37 0.49640342424399936 38-39 0.47114675921668353 40-41 0.45166558487283476 42-43 0.48227568549521055 44-45 0.49176081827152 46-47 0.4858298336081134 48-49 0.5040670561124462 50-51 0.4997798649512571 52-53 0.49931338301143857 54-55 0.4893617682953106 56-57 0.46881434951759104 58-59 0.4519321459813025 60-61 0.4113704306427988 62-63 0.4814315753183961 64-65 0.5056886361889581 66-67 0.5154181166480296 68-69 0.5285462512400645 70-71 0.5252142373842181 72-73 0.513218987503171 74-75 0.5152181958166788 76-77 0.4894061951467219 78-79 0.4699694476542844 80-81 0.47592264574339677 82-83 0.48420825353160146 84-85 0.48600754101375854 86-87 0.4856743396281739 88-89 0.4918718854000482 90-91 0.5059329838717201 92-93 0.5121305296435945 94-95 0.5189278379095211 96-97 0.5108865778040784 98-99 0.513463335185933 100-101 0.49167196456869744 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2250891.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.2022395443462 #Duplication Level Percentage of deduplicated Percentage of total 1 71.76065693331975 30.28460433759608 2 14.12312123939374 11.920546913174764 3 5.43705769620695 6.883680339352703 4 2.63621625894007 4.450169202019561 5 1.537715869326955 3.2447526734239354 6 0.9812901516249936 2.4847585224831468 7 0.6883893431040211 2.0336100370215715 8 0.4963109279069048 1.6756346134403146 9 0.3521363833960091 1.3374849603922299 >10 1.8514437972114586 13.836127451026655 >50 0.09388402495697785 2.705658160433112 >100 0.035327202652457125 2.633913177729842 >500 0.0021148104786006846 0.6075229693486984 >1k 0.0020090699546706503 2.0242733168499822 >5k 0.0011631457632303764 3.5246506692403106 >10k+ 0.0011631457632303764 10.35261265646711 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 53538 2.378524770857407 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 35128 1.5606264363756397 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 25347 1.1260874027218555 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 18193 0.8082577077255185 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 17655 0.7843560616662468 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 15533 0.6900822829714989 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 14617 0.6493872870787613 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 14515 0.6448557482348102 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 13394 0.595053247802759 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 12436 0.5524923241507473 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 11636 0.516950843021719 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 9772 0.43413919199108264 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8643 0.3839812767477412 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 7713 0.3426643049352457 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 7695 0.34186462160984254 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 7640 0.3394211447822218 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 7238 0.321561550514885 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7124 0.31649688945399845 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 6462 0.2870863138197274 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 5923 0.2631402409090445 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 5534 0.24585819571005438 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 5240 0.23279670139513642 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 4639 0.2060961636969538 No Hit GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 4090 0.181705822272158 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4059 0.18032858987840814 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 4022 0.17868479637619059 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 3014 0.13390253015361472 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 2923 0.12985968667518774 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 2758 0.12252925619232562 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 2745 0.1219517071239789 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 2325 0.10329242953123897 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 2314 0.10280373416571482 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 4.4426851411285574E-5 0.0 0.0 0.0 0.0 7 1.3328055423385672E-4 0.0 0.0 0.0 0.0 8 1.3328055423385672E-4 0.0 0.0 0.0 0.0 9 1.3328055423385672E-4 0.0 0.0 4.4426851411285574E-5 0.0 10-11 1.3328055423385672E-4 0.0 0.0 1.1106712852821394E-4 0.0 12-13 2.6656110846771345E-4 0.0 0.0 1.777074056451423E-4 8.885370282257115E-5 14-15 3.554148112902846E-4 0.0 0.0 2.8877453417335626E-4 8.885370282257115E-5 16-17 3.776282369959274E-4 0.0 0.0 3.554148112902846E-4 8.885370282257115E-5 18-19 5.109087912297841E-4 0.0 0.0 5.109087912297842E-4 8.885370282257115E-5 20-21 6.21975919757998E-4 0.0 0.0 7.108296225805692E-4 8.885370282257115E-5 22-23 6.664027711692837E-4 0.0 0.0 0.0010218175824595684 1.3328055423385672E-4 24-25 9.329638796369971E-4 0.0 0.0 0.0013994458194554957 1.777074056451423E-4 26-27 9.329638796369971E-4 0.0 0.0 0.00231019627338685 1.777074056451423E-4 28-29 0.0011106712852821392 0.0 0.0 0.00597541151481791 1.777074056451423E-4 30-31 0.0011995249881047105 0.0 0.0 0.01363904338326467 1.777074056451423E-4 32-33 0.0011995249881047105 0.0 0.0 0.029166227951508982 1.777074056451423E-4 34-35 0.001243951839515996 0.0 0.0 0.04920273793799877 1.777074056451423E-4 36-37 0.0013772323937498528 0.0 0.0 0.07839117931521339 1.777074056451423E-4 38-39 0.001488299522278067 0.0 0.0 0.12510601357418016 1.777074056451423E-4 40-41 0.0015105129479837096 0.0 0.0 0.17544163622316675 1.777074056451423E-4 42-43 0.0017104337793344946 0.0 0.0 0.2353290319255797 1.777074056451423E-4 44-45 0.0018659277592739942 0.0 0.0 0.30767815944885823 1.777074056451423E-4 46-47 0.001999208313507851 0.0 0.0 0.3983755766049978 1.777074056451423E-4 48-49 0.002088062016330422 0.0 0.0 0.48398611927454505 2.2213425705642787E-4 50-51 0.002088062016330422 0.0 0.0 0.5835244798615304 2.2213425705642787E-4 52-53 0.0021324888677417076 0.0 0.0 0.6870612570755315 2.6656110846771345E-4 54-55 0.002221342570564279 0.0 0.0 0.8052588952552566 2.6656110846771345E-4 56-57 0.0022657694219755642 0.0 0.0 0.96537326774153 2.6656110846771345E-4 58-59 0.0022657694219755642 0.0 0.0 1.1143142870978648 2.6656110846771345E-4 60-61 0.0022657694219755642 0.0 0.0 1.2800264428619599 2.6656110846771345E-4 62-63 0.0022657694219755642 0.0 0.0 1.4565787503704088 2.6656110846771345E-4 64-65 0.00231019627338685 0.0 0.0 1.6391731096707924 2.6656110846771345E-4 66-67 0.0026656110846771347 0.0 0.0 1.8642173254946597 2.6656110846771345E-4 68-69 0.0027544647874997055 0.0 0.0 2.128668158520337 2.6656110846771345E-4 70-71 0.0027766782132053486 0.0 0.0 2.406335979840872 2.6656110846771345E-4 72-73 0.0028655319160279195 0.0 0.0 2.678339377606468 2.8877453417335626E-4 74-75 0.0029543856188504907 0.0 0.0 3.0006783980210505 3.332013855846418E-4 76-77 0.0030210258959674193 0.0 0.0 3.312066199562751 3.776282369959274E-4 78-79 0.0030210258959674193 0.0 0.0 3.738008637468451 3.9984166270157017E-4 80-81 0.003443080984374632 0.0 0.0 4.184343000171932 3.9984166270157017E-4 82-83 0.0034652944100802747 0.0 0.0 4.588338573480457 3.9984166270157017E-4 84-85 0.003554148112902846 0.0 0.0 5.035805820894925 3.9984166270157017E-4 86-87 0.003643001815725417 0.0 0.0 5.492247292294474 4.2205508840721293E-4 88-89 0.003643001815725417 0.0 0.0 6.007376634408329 4.4426851411285574E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTTGCGT 1435 0.0 36.971024 3 CTTTGCG 1650 0.0 31.866505 2 GTAAGCG 585 0.0 27.235832 94-95 TTGCGTT 1935 0.0 27.172989 4 TAACACG 120 1.7438401E-4 23.684565 4 GCTTTGC 2325 0.0 23.06807 1 GAGTACT 7295 0.0 22.328749 12-13 GTACATG 8200 0.0 21.705667 1 AGTACTT 7485 0.0 21.445646 12-13 TACATGG 8240 0.0 21.44258 2 GTACTTT 7645 0.0 21.27577 14-15 ACATGGG 8610 0.0 19.860924 3 TATAGGG 315 3.3287506E-10 19.549164 2 TACTTTT 8425 0.0 18.996904 14-15 ACTTTTT 8695 0.0 18.898605 16-17 AGAGTAC 13100 0.0 18.832052 10-11 GTATAGG 390 1.6370905E-11 18.255022 1 CCGTTTA 160 0.0012168277 17.75671 9 CATGGGG 4725 0.0 17.644373 4 TCGCGCT 205 6.020855E-10 16.24182 60-61 >>END_MODULE