##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938927_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2250891 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.15817380761663 33.0 31.0 34.0 30.0 34.0 2 32.39371475562344 34.0 31.0 34.0 31.0 34.0 3 32.483207316569306 34.0 31.0 34.0 30.0 34.0 4 36.00515795744885 37.0 35.0 37.0 35.0 37.0 5 35.93951772875719 37.0 35.0 37.0 35.0 37.0 6 36.00466437512967 37.0 35.0 37.0 35.0 37.0 7 35.947772237749405 37.0 35.0 37.0 35.0 37.0 8 35.93260357787205 37.0 35.0 37.0 35.0 37.0 9 37.57822968771033 39.0 37.0 39.0 35.0 39.0 10-11 37.48510745300417 39.0 37.0 39.0 34.5 39.0 12-13 37.413423395446515 39.0 37.0 39.0 34.0 39.0 14-15 38.644270646601726 40.0 38.0 41.0 34.0 41.0 16-17 38.79377211068861 40.0 38.0 41.0 34.5 41.0 18-19 38.861528612447245 40.0 38.0 41.0 35.0 41.0 20-21 38.81762088879471 40.0 38.0 41.0 34.0 41.0 22-23 38.744206405374584 40.0 38.0 41.0 34.0 41.0 24-25 38.62651034634729 40.0 38.0 41.0 34.0 41.0 26-27 38.438315093889486 40.0 38.0 41.0 34.0 41.0 28-29 38.317939429319324 40.0 38.0 41.0 34.0 41.0 30-31 38.17455198852366 40.0 38.0 41.0 33.5 41.0 32-33 38.058916891133336 40.0 37.0 41.0 33.0 41.0 34-35 37.91201217651144 40.0 37.0 41.0 33.0 41.0 36-37 37.75843965789547 40.0 37.0 41.0 33.0 41.0 38-39 37.604491065982316 40.0 37.0 41.0 33.0 41.0 40-41 37.53311866278732 40.0 37.0 41.0 32.0 41.0 42-43 37.31746383987497 40.0 36.5 41.0 31.5 41.0 44-45 37.04794856792266 40.0 36.0 41.0 31.0 41.0 46-47 36.72572461305323 39.0 36.0 41.0 30.5 41.0 48-49 36.337231123141905 39.0 35.0 41.0 30.0 41.0 50-51 36.04510280595551 39.0 35.0 40.0 29.0 41.0 52-53 35.826976073030636 39.0 35.0 40.0 28.5 41.0 54-55 35.54330351847335 38.0 35.0 40.0 28.0 41.0 56-57 35.32131986844321 38.0 34.5 40.0 28.0 41.0 58-59 34.998467495760565 38.0 34.0 40.0 27.0 41.0 60-61 34.49254606287022 37.0 34.0 40.0 26.0 41.0 62-63 34.09659485954673 37.0 33.0 39.5 26.0 41.0 64-65 33.66822182860032 36.0 33.0 39.0 25.5 40.5 66-67 33.10778154073209 36.0 32.0 39.0 23.5 40.0 68-69 32.55569749934581 35.0 32.0 38.0 22.5 40.0 70-71 31.901600299614685 35.0 31.0 37.0 21.5 39.0 72-73 31.341619607524308 34.5 30.5 36.5 20.0 39.0 74-75 30.850052268190687 34.0 30.0 36.0 18.0 38.5 76-77 29.00539341976133 32.5 28.0 34.5 13.5 36.0 78-79 29.97840832807986 34.0 29.5 35.0 15.5 37.0 80-81 29.92125629361884 34.0 30.0 35.0 12.0 36.5 82-83 29.490670139069373 34.0 29.0 35.0 7.5 36.0 84-85 29.01942986133047 34.0 29.0 35.0 4.0 35.5 86-87 28.441027131033888 33.0 28.5 35.0 2.0 35.0 88-89 27.70465340169737 33.0 26.5 35.0 2.0 35.0 90-91 27.075772660693033 32.5 25.5 34.0 2.0 35.0 92-93 26.65183853860538 32.0 25.0 34.0 2.0 35.0 94-95 26.036875841611167 32.0 24.5 34.0 2.0 35.0 96-97 25.07462711432939 31.5 20.5 34.0 2.0 35.0 98-99 23.81715418472063 31.0 4.5 34.0 2.0 35.0 100-101 18.157962113669655 23.5 2.0 30.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.09824748388217586 1101 2 0.1631547806470266 1101 3 0.12453644259300489 1101 4 0.053397565303470174 1101 5 0.11065710890643032 1101 6 0.07253569987921082 1101 7 0.14839696886033238 1101 8 0.08569547553621248 1101 9 0.1400016763700691 1101 10-11 0.2576928197769135 1101 12-13 0.1899308386742078 1101 14-15 0.22148034436781217 1101 16-17 0.24442299330372919 1101 18-19 0.29427179481947263 1101 20-21 0.22266817914223225 1101 22-23 0.20269618057231042 1101 24-25 0.19706661309859896 1101 26-27 0.18408900118765104 1101 28-29 0.2959195054774213 1101 30-31 0.2619176085273409 1101 32-33 0.3105644318208931 1101 34-35 0.3684243564779379 1101 36-37 0.35871310985250204 1101 38-39 0.3228973981100367 1101 40-41 0.36065582253258555 1101 42-43 0.32221184333904773 1101 44-45 0.41376559908659516 1101 46-47 0.4411642028007918 1101 48-49 0.33030447212906466 1101 50-51 0.2736389567856321 1101 52-53 0.2959489763582255 1101 54-55 0.35388555418975187 1101 56-57 0.26912069373992864 1101 58-59 0.3278374660882051 1101 60-61 0.3392012718824233 1101 62-63 0.42718645177509273 1101 64-65 0.26721443567360126 1101 66-67 0.317549467781582 1101 68-69 0.28417609695234347 1101 70-71 0.41143832701080996 1101 72-73 0.5215147392829884 1101 74-75 0.5041921416603081 1101 76-77 0.22092907403766304 1101 78-79 0.5792625122398434 1101 80-81 0.6810420743858678 1101 82-83 0.48861095665936816 1101 84-85 0.5750892771096687 1101 86-87 0.7667535149143454 1101 88-89 0.8264773498755211 1101 90-91 0.8398782797049869 1101 92-93 0.6521631867582833 1101 94-95 0.8711009966919825 1101 96-97 0.9129685504811071 1101 98-99 1.3311893776548693 1101 100-101 1.6517924245603162 1102 1 0.07797889207027708 1102 2 0.13294506337133782 1102 3 0.10250849951746233 1102 4 0.022938970998154673 1102 5 0.04864602434220444 1102 6 0.04674087503281754 1102 7 0.016279783599060238 1102 8 0.09016921459027571 1102 9 0.13086184190353123 1102 10-11 0.1100676881279341 1102 12-13 0.13306418186957814 1102 14-15 0.186673612500023 1102 16-17 0.15544584998405497 1102 18-19 0.10273975434416371 1102 20-21 0.10015194863837706 1102 22-23 0.12187476077304638 1102 24-25 0.11462342218804622 1102 26-27 0.020433165091084504 1102 28-29 0.020927832391123502 1102 30-31 0.1217161065195782 1102 32-33 0.1693035742697333 1102 34-35 0.12372043640885977 1102 36-37 0.13744061322979206 1102 38-39 0.16686219992018891 1102 40-41 0.1427666579406619 1102 42-43 0.38509620716283877 1102 44-45 0.3577416352523741 1102 46-47 0.31762548311727556 1102 48-49 0.24563429699406925 1102 50-51 0.2788973562690842 1102 52-53 0.22035990536267747 1102 54-55 0.33603374441181444 1102 56-57 0.3472564512750935 1102 58-59 0.2670919328493966 1102 60-61 0.06816220795776928 1102 62-63 0.3166146905258529 1102 64-65 0.24628771530532134 1102 66-67 0.21317334899791973 1102 68-69 0.1032508698462351 1102 70-71 0.25806529895423225 1102 72-73 0.36868203023558976 1102 74-75 0.46445287640112554 1102 76-77 0.1628515258159915 1102 78-79 0.5534676873934501 1102 80-81 0.49918309322795196 1102 82-83 0.2943580157237058 1102 84-85 0.5733983088803356 1102 86-87 0.670487763556924 1102 88-89 0.5009695709564745 1102 90-91 0.4354475242724902 1102 92-93 0.5546873580675538 1102 94-95 0.7461757760985464 1102 96-97 0.7749757739624457 1102 98-99 1.0094341758923555 1102 100-101 1.1668857697281005 1103 1 0.1002048482232567 1103 2 0.1042483576126969 1103 3 0.1095281369451655 1103 4 0.11352215002108323 1103 5 0.0734118154402097 1103 6 0.10859328570423799 1103 7 0.11879287140739336 1103 8 0.10841419092934501 1103 9 0.12685937293706218 1103 10-11 0.11246441446129296 1103 12-13 0.15319357400088762 1103 14-15 0.18280647947302242 1103 16-17 0.10750023582864543 1103 18-19 0.10995839504095528 1103 20-21 0.23229857781333152 1103 22-23 0.10709125255458929 1103 24-25 0.10099518452716438 1103 26-27 0.11742925589418718 1103 28-29 0.16171961621938635 1103 30-31 0.07542812901460394 1103 32-33 0.138813324401184 1103 34-35 0.14900436755213065 1103 36-37 0.07849301018472943 1103 38-39 0.15594833941679553 1103 40-41 0.2888244271837479 1103 42-43 0.3564241910688324 1103 44-45 0.2814441705151651 1103 46-47 0.39154210977753934 1103 48-49 0.3563634422287336 1103 50-51 0.45180479288751485 1103 52-53 0.4514057869894472 1103 54-55 0.5900967944998285 1103 56-57 0.5868837059215508 1103 58-59 0.5222062368523197 1103 60-61 0.532104095802687 1103 62-63 0.6588517341671221 1103 64-65 0.5630602274786938 1103 66-67 0.5345054478480264 1103 68-69 0.6467896074728259 1103 70-71 0.5765476847073749 1103 72-73 0.5653588699585193 1103 74-75 0.6100074794233201 1103 76-77 0.37405891899890165 1103 78-79 0.6836534313982057 1103 80-81 0.5885046989705849 1103 82-83 0.715875629970558 1103 84-85 0.8625754343632721 1103 86-87 1.004365641160195 1103 88-89 0.8565230309386429 1103 90-91 0.9156313251091923 1103 92-93 0.8079522233031327 1103 94-95 1.0092114618082597 1103 96-97 1.0290261363061362 1103 98-99 1.3419092004677147 1103 100-101 1.5630410402856754 1104 1 0.03139803004144426 1104 2 0.02034954808888756 1104 3 0.0028398252568493376 1104 4 0.018051370800300504 1104 5 0.0031980708514609546 1104 6 0.05199967098562297 1104 7 0.026447092186614896 1104 8 -0.019286025520869998 1104 9 0.062129935707631034 1104 10-11 0.07202069151757229 1104 12-13 0.09348307616539131 1104 14-15 0.08348153358557653 1104 16-17 0.14291554968146158 1104 18-19 0.16465807036563262 1104 20-21 0.1255872682895145 1104 22-23 0.09220353610437115 1104 24-25 0.04649843128041198 1104 26-27 0.06841004593747613 1104 28-29 -0.02585749417022498 1104 30-31 -0.023279933755951276 1104 32-33 0.01744427678212901 1104 34-35 0.19834419439195727 1104 36-37 0.12688722014144105 1104 38-39 0.1326555904990414 1104 40-41 0.2303869271837371 1104 42-43 0.11985763262728 1104 44-45 0.20306484151083026 1104 46-47 0.26410839765632943 1104 48-49 0.19723871279150984 1104 50-51 0.11961323444595706 1104 52-53 0.2276625510587209 1104 54-55 0.4817539914695246 1104 56-57 0.34372759120293495 1104 58-59 0.3703244727830608 1104 60-61 0.568191758140351 1104 62-63 0.521013530703911 1104 64-65 0.5051570889505612 1104 66-67 0.38145891105148877 1104 68-69 0.47340486721308395 1104 70-71 0.5114096976943863 1104 72-73 0.39790216000180934 1104 74-75 0.46352750106834506 1104 76-77 0.4846271008170824 1104 78-79 0.6259613318311068 1104 80-81 0.527820175161061 1104 82-83 0.5800761169835411 1104 84-85 0.6897331724585101 1104 86-87 0.5280290610736138 1104 88-89 0.4932045785576875 1104 90-91 0.4861575696979408 1104 92-93 0.5819497882381981 1104 94-95 0.7826584315052294 1104 96-97 0.803417370072367 1104 98-99 1.015066668000184 1104 100-101 1.3727501853073214 1105 1 -0.03314085369387243 1105 2 0.0101358035001482 1105 3 -0.026458257612659963 1105 4 -0.02405979184657525 1105 5 0.03335615681312021 1105 6 0.05148753431276987 1105 7 1.3486274938401266E-4 1105 8 0.11060343026144182 1105 9 0.13027310206508247 1105 10-11 0.10939700567960386 1105 12-13 0.10441187950491582 1105 14-15 0.04646758027697473 1105 16-17 -0.0985665545238561 1105 18-19 0.05783409077379531 1105 20-21 0.10934867057770958 1105 22-23 0.12739119071166982 1105 24-25 0.09777007630205503 1105 26-27 -0.055019723697647294 1105 28-29 0.12008696315816536 1105 30-31 0.1428214499793583 1105 32-33 0.2653377523541849 1105 34-35 0.2248206940827444 1105 36-37 0.20216647957248313 1105 38-39 0.2198444433128941 1105 40-41 0.3365393313272165 1105 42-43 0.35885152563903233 1105 44-45 0.24820050941435312 1105 46-47 0.34006406401377376 1105 48-49 0.23978953994054564 1105 50-51 0.29399094007366244 1105 52-53 0.2977044882881543 1105 54-55 0.24764472276203975 1105 56-57 0.26272786176570406 1105 58-59 0.3222464347496654 1105 60-61 0.34395319908036015 1105 62-63 0.21318073849612773 1105 64-65 0.12063907720040135 1105 66-67 0.18968788445903328 1105 68-69 0.29008892720072055 1105 70-71 0.5109941905824549 1105 72-73 0.50268725585833 1105 74-75 0.3702703118290138 1105 76-77 0.1394856351399021 1105 78-79 0.332564996024054 1105 80-81 0.44582999272444823 1105 82-83 0.3918465637986337 1105 84-85 0.4741091387541161 1105 86-87 0.632099098179367 1105 88-89 0.7625063333505153 1105 90-91 0.6092491358698631 1105 92-93 0.9202063977001664 1105 94-95 0.8800803548200058 1105 96-97 0.9632716526945089 1105 98-99 1.136423733553677 1105 100-101 1.0761022647754714 1106 1 0.010101958224773 1106 2 0.051516801077241325 1106 3 -0.04475195729861525 1106 4 -0.010412311643747785 1106 5 0.09860408572099288 1106 6 0.017764545674410215 1106 7 0.05802227719603792 1106 8 -0.0023587809920613267 1106 9 3.280195877337633E-4 1106 10-11 0.032045072870822366 1106 12-13 0.04742179093451426 1106 14-15 -0.06398771891446131 1106 16-17 -0.1737788641000222 1106 18-19 -0.08830728163371049 1106 20-21 0.06388991430252844 1106 22-23 0.022826829581589436 1106 24-25 0.05783412709466518 1106 26-27 -0.071780263592224 1106 28-29 0.009700616154546537 1106 30-31 -0.040003749592486315 1106 32-33 0.09577969061176361 1106 34-35 0.03570010712803651 1106 36-37 -0.0958721412321637 1106 38-39 0.08004761961911555 1106 40-41 0.17037761223442516 1106 42-43 0.2395100264016108 1106 44-45 0.18864171498915283 1106 46-47 0.1562410052231371 1106 48-49 0.13967570040481547 1106 50-51 0.04075081886939813 1106 52-53 0.10382262117659735 1106 54-55 0.013649525624053638 1106 56-57 0.0051312255376601 1106 58-59 0.11610276323410318 1106 60-61 0.1650049020625488 1106 62-63 0.1116331145358771 1106 64-65 0.034575693401784235 1106 66-67 0.17355328305110618 1106 68-69 0.18601933749466326 1106 70-71 0.30958758700510813 1106 72-73 0.22928825197005764 1106 74-75 0.2562410786278697 1106 76-77 0.17376613642968053 1106 78-79 0.26461845744493573 1106 80-81 0.1562695434467294 1106 82-83 0.06830100229833036 1106 84-85 0.20266416617918992 1106 86-87 0.4221158951428592 1106 88-89 0.7394537747295757 1106 90-91 0.7403210339477901 1106 92-93 0.7597351816067466 1106 94-95 0.867040180222542 1106 96-97 1.001770554091408 1106 98-99 0.9697077651954693 1106 100-101 0.9622084094575847 1107 1 -0.014299656281245632 1107 2 0.048418241782584914 1107 3 0.047700595117625255 1107 4 -0.01320886670993815 1107 5 0.054981957010348026 1107 6 0.0156846627956142 1107 7 0.02134113395565862 1107 8 -0.036643724128566646 1107 9 -0.0015576154799248343 1107 10-11 0.06689169888857549 1107 12-13 0.027704513511828566 1107 14-15 0.004769156435699529 1107 16-17 0.06176664509505514 1107 18-19 -0.008844693762135591 1107 20-21 0.04953152504627667 1107 22-23 0.15554685101019317 1107 24-25 0.026908955440937632 1107 26-27 0.0643726278375496 1107 28-29 -0.05387883937906679 1107 30-31 -0.06475848617201052 1107 32-33 0.056014096601956 1107 34-35 0.06434542289319012 1107 36-37 0.07357666526839068 1107 38-39 -0.020377271908813555 1107 40-41 -0.029734131374816286 1107 42-43 0.058097292741940976 1107 44-45 -0.07165106758007056 1107 46-47 -0.19580023256480672 1107 48-49 -0.22526461439932177 1107 50-51 -0.26390949282677667 1107 52-53 -0.4488001332164657 1107 54-55 -0.35436266495963054 1107 56-57 -0.37255644851860126 1107 58-59 -0.20042568077959544 1107 60-61 -0.1243502156516243 1107 62-63 0.029958555273942977 1107 64-65 -0.10311737869891147 1107 66-67 -0.055513857171277436 1107 68-69 -0.049330083646864864 1107 70-71 -0.030080371920679028 1107 72-73 -0.0950851454854984 1107 74-75 -0.08015339573755398 1107 76-77 -0.18058072064073727 1107 78-79 0.1778168805616538 1107 80-81 0.19113661660250258 1107 82-83 0.18385503794996438 1107 84-85 0.19909409588193228 1107 86-87 0.39232574412029564 1107 88-89 0.6054934299090426 1107 90-91 0.5509981204759917 1107 92-93 0.556073201424109 1107 94-95 0.7944366870334854 1107 96-97 1.039450846730844 1107 98-99 0.9081640267225417 1107 100-101 0.8819856992303343 1108 1 0.06737119863439034 1108 2 0.09469924764613324 1108 3 0.11669900529250299 1108 4 0.0314113702252925 1108 5 -0.027723993722169382 1108 6 -0.01570327162837515 1108 7 0.06201415992280346 1108 8 0.09158849290820115 1108 9 0.15209385382619445 1108 10-11 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-1.0207058510597662 2316 60-61 -0.9259911422925455 2316 62-63 -1.0599211596057785 2316 64-65 -0.9989544245359525 2316 66-67 -1.1176190942765132 2316 68-69 -0.969382553699333 2316 70-71 -0.9643863812266886 2316 72-73 -0.9118755622759132 2316 74-75 -1.0258716414557973 2316 76-77 -1.0145674546274712 2316 78-79 -0.9834344806897093 2316 80-81 -1.073748048282166 2316 82-83 -1.0135932344983125 2316 84-85 -1.2441095473217132 2316 86-87 -1.1427772159826617 2316 88-89 -1.3419201925045812 2316 90-91 -1.4795335100556386 2316 92-93 -1.5243371539862416 2316 94-95 -1.429140186543389 2316 96-97 -1.3406625083825077 2316 98-99 -1.4073765138179972 2316 100-101 -0.8866659194212829 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 7.0 4 15.0 5 38.0 6 96.0 7 251.0 8 647.0 9 1285.0 10 2142.0 11 3336.0 12 4697.0 13 6375.0 14 8021.0 15 10541.0 16 13827.0 17 19546.0 18 20025.0 19 15213.0 20 14499.0 21 16257.0 22 17381.0 23 19093.0 24 21743.0 25 25302.0 26 30320.0 27 36145.0 28 44035.0 29 53048.0 30 63826.0 31 77510.0 32 96493.0 33 122932.0 34 166712.0 35 241016.0 36 372466.0 37 481491.0 38 235640.0 39 8919.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.72233918045188 19.044868546879236 15.454439218988625 20.77835305368026 2 15.83994960217976 22.883293771222153 35.467999116794196 25.808757509803897 3 22.935939590144525 23.67391401893739 30.232161397419954 23.15798499349813 4 13.27514304335483 16.918322566485894 38.03653753113767 31.769996859021603 5 13.411844465147357 38.34583727066304 33.49371426692807 14.748603997261528 6 30.585177158734027 36.43894795438784 15.793256981346499 17.182617905531632 7 27.118061247745896 33.32604732970188 20.626498573231665 18.929392849320557 8 26.432466076766936 30.126869759575207 19.317416969546727 24.123247194111133 9 24.26834529082039 19.08364287742054 22.268248440284314 34.379763391474754 10-11 26.112748240585614 24.923730202839675 26.109816068392472 22.853705488182236 12-13 29.0084459887218 23.1448790723318 25.794785265035046 22.051889673911354 14-15 23.842804033281322 25.116532614915464 27.536483638341053 23.50417971346216 16-17 21.6738984449955 28.05054758701181 27.259950165039154 23.01560380295353 18-19 21.432677766345194 28.974141623083632 27.626110900822614 21.96706970974856 20-21 23.969330367396733 25.14333212936566 26.621591183224773 24.26574632001283 22-23 22.659004811872276 25.312065311025723 28.11251188973611 23.916417987365893 24-25 24.889371562055107 25.622525840328525 27.08850119541577 22.399601402200595 26-27 23.459426391465954 24.0036998690074 30.49946232391136 22.03741141561529 28-29 21.774866041936285 28.493205579479415 27.707339004865183 22.02458937371912 30-31 22.907284270984245 25.248623767210404 27.170307225005562 24.673784736799785 32-33 23.240774431103063 25.605749012280025 28.293995577751215 22.859480978865705 34-35 25.08027848536592 25.048309275336432 27.453064800855593 22.418347438442055 36-37 23.73657084035161 24.404326659552293 29.852222308874506 22.00688019122159 38-39 22.77635768978021 26.295485334635483 28.73303318207286 22.195123793511453 40-41 22.792363166136436 26.869138480315115 27.5818121918943 22.75668616165415 42-43 22.341556824505417 26.83584599475512 25.902986425741442 24.91961075499802 44-45 23.572208754443608 24.500926429646107 27.005363095778893 24.92150172013139 46-47 25.287740083288185 26.51738147138904 27.06097381911221 21.133904626210565 48-49 24.09405947192344 25.833766510722807 27.32165309377513 22.750520923578623 50-51 21.004013102448514 26.745619596272917 29.53068959343403 22.719677707844543 52-53 23.30348293187009 28.118331807270984 25.252555543560305 23.325629717298614 54-55 22.277200450843686 25.614701022839398 26.867071750697836 25.24102677561908 56-57 24.236513451784205 25.06087589314631 26.55779422459817 24.144816430471312 58-59 26.28370719189683 25.137019028390533 25.98217980708378 22.597093972628848 60-61 23.45432933508289 26.165515884043405 28.906224488911903 21.473930291961803 62-63 22.07630278451162 26.523266758083512 28.232434558945542 23.167995898459324 64-65 21.900734470907274 28.59194125654413 26.518322676722622 22.98900159582597 66-67 23.351201568666827 25.878271045528344 25.424127160287867 25.34640022551696 68-69 22.251352281941067 26.02203636444415 27.060744393722302 24.665866959892487 70-71 24.845566676833013 25.975430612906315 25.75531772869449 23.423684981566183 72-73 23.42610104176524 24.512915107839518 28.87314401274873 23.187839837646514 74-75 21.837263554743434 28.94053954633965 26.08071648071808 23.14148041819884 76-77 22.894544283823443 25.73458410049953 25.97871359763501 25.392158018042014 78-79 23.0864512643551 26.11435919889338 27.04697838979408 23.752211146957443 80-81 24.711724499474652 25.657574687289664 26.123058263749595 23.50764254948609 82-83 23.281780414955676 25.07995722582746 28.512620113546145 23.125642245670715 84-85 22.51584707901948 26.605588549941178 27.367376786406076 23.511187584633262 86-87 22.496946702872666 27.057470911689062 26.591088530163997 23.85449385527427 88-89 22.108421221960363 26.926304295783243 25.165185104369765 25.800089377886625 90-91 23.104560303661952 24.81815584819311 26.24316014662157 25.83412370152337 92-93 24.373792422645078 26.441018245663606 26.455612466352214 22.729576865339105 94-95 23.269416466947284 25.703434655053954 26.87809339203369 24.14905548596507 96-97 20.537911431517564 26.211886759509902 29.02839364500547 24.22180816396707 98-99 22.421076809139137 27.30196619916291 25.389434672758476 24.887522318939478 100-101 22.121047135491466 24.562888305858564 27.121394776762102 26.194669781887868 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 743.0 1 532.5 2 343.5 3 389.5 4 364.0 5 385.5 6 655.0 7 1391.0 8 2345.0 9 2896.0 10 2675.5 11 2291.0 12 2346.0 13 2459.0 14 2628.0 15 2823.5 16 2942.5 17 3076.0 18 3239.5 19 3485.0 20 3777.0 21 4214.0 22 4869.5 23 5710.5 24 6692.0 25 7927.5 26 9366.0 27 10938.5 28 12649.5 29 14734.0 30 17046.0 31 19592.5 32 22319.5 33 24748.5 34 27400.5 35 30184.0 36 33824.5 37 37410.5 38 39846.5 39 41954.0 40 43462.0 41 44788.0 42 46798.0 43 50650.5 44 55427.5 45 60508.0 46 70771.0 47 122879.0 48 209366.0 49 202805.5 50 123180.0 51 84446.5 52 75742.5 53 75100.0 54 75229.5 55 72936.0 56 67899.5 57 60967.0 58 55939.5 59 51253.0 60 43842.0 61 35976.5 62 28974.5 63 22251.0 64 16376.0 65 12019.5 66 8531.0 67 6315.0 68 4596.0 69 3173.5 70 2235.0 71 1378.5 72 869.5 73 565.0 74 332.0 75 203.5 76 128.0 77 56.0 78 26.5 79 18.5 80 9.5 81 6.5 82 5.0 83 3.0 84 1.0 85 0.5 86 1.5 87 1.0 88 0.0 89 0.5 90 0.5 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3942883062751595 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 8.44110176814426E-4 16-17 0.002732251361794063 18-19 6.664027711692836E-4 20-21 0.0 22-23 0.0 24-25 2.2213425705642787E-5 26-27 2.2213425705642787E-5 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.012794933206450244 36-37 0.022990895605340286 38-39 0.011017859149998824 40-41 0.006286399474696909 42-43 0.005486716149293768 44-45 0.0020214217392134936 46-47 0.0013994458194554957 48-49 0.0035985749643141314 50-51 6.21975919757998E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 7.774698996974975E-4 60-61 0.0013994458194554957 62-63 0.0013105921166329244 64-65 8.44110176814426E-4 66-67 0.002776678213205348 68-69 0.002932172193144848 70-71 2.2213425705642787E-5 72-73 0.0 74-75 0.0 76-77 0.09260777176682479 78-79 0.0035319346871972037 80-81 2.6656110846771345E-4 82-83 0.0 84-85 1.3328055423385672E-4 86-87 0.09296318657811506 88-89 0.43736013871840085 90-91 0.39062309103372844 92-93 0.0 94-95 0.0010884578595764965 96-97 0.0 98-99 0.0 100-101 3.332013855846418E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2250891.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.59048145029652 #Duplication Level Percentage of deduplicated Percentage of total 1 70.10393277444706 28.455523828740276 2 14.919497341134317 12.111791601461217 3 5.6818711486407425 6.918896563856312 4 2.816697569619525 4.573244418029465 5 1.640719691625176 3.329880110402397 6 1.0645001342953082 2.592514377297112 7 0.7052145284331341 2.003749806439132 8 0.526347593348864 1.7091761779388228 9 0.3891305896361543 1.4215498182332345 >10 2.0045435314576543 14.348629898883955 >50 0.10066371849602455 2.7597629073266514 >100 0.040091799640760345 2.8577366417901326 >500 0.0024092055315479504 0.6757345139482996 >1k 0.0019711681621755956 2.069164690454765 >5k 0.0010950934234308864 3.0967238773343104 >10k+ 0.0013141121081170636 11.075920767863936 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 58538 2.600659027913835 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 31978 1.42068185443009 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 23212 1.0312360749587608 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 18620 0.8272279732781375 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 17446 0.7750708497212881 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 17230 0.7654746498164505 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 16425 0.7297110344303656 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 14920 0.6628486230563808 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 14601 0.6486764574561807 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 12983 0.5767938118727206 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 12273 0.5452507473707079 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 10949 0.48642959610216574 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8629 0.38335930082798325 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 8189 0.3638114862070176 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 7784 0.3458186113854469 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 7709 0.3424865975296005 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7376 0.3276924560096424 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 7112 0.315963767237063 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 6155 0.2734472704364627 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 5727 0.25443257803243247 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 5627 0.24998989289130394 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 5359 0.23808349671307938 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 4998 0.22204540335360531 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4468 0.19849917210562396 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 3926 0.17441981864070716 No Hit GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 3702 0.1644682039245792 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 3619 0.1607807752574425 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 3197 0.14203264396187998 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 2644 0.11746459513143906 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 2624 0.11657605810321335 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 2559 0.11368831276147978 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 2445 0.10862365170059324 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 2325 0.10329242953123897 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 4.4426851411285574E-5 0.0 0.0 0.0 0.0 3 4.4426851411285574E-5 0.0 0.0 0.0 0.0 4 4.4426851411285574E-5 0.0 0.0 0.0 0.0 5 4.4426851411285574E-5 0.0 0.0 0.0 0.0 6 8.885370282257115E-5 0.0 0.0 0.0 0.0 7 1.3328055423385672E-4 0.0 0.0 0.0 0.0 8 1.3328055423385672E-4 0.0 0.0 0.0 0.0 9 1.3328055423385672E-4 0.0 0.0 8.885370282257115E-5 0.0 10-11 1.3328055423385672E-4 0.0 0.0 1.9992083135078509E-4 0.0 12-13 2.2213425705642787E-4 0.0 0.0 2.2213425705642787E-4 4.4426851411285574E-5 14-15 3.332013855846418E-4 0.0 0.0 2.8877453417335626E-4 6.664027711692836E-5 16-17 3.776282369959274E-4 0.0 0.0 3.10987959878999E-4 8.885370282257115E-5 18-19 5.109087912297841E-4 0.0 0.0 4.2205508840721293E-4 8.885370282257115E-5 20-21 6.21975919757998E-4 0.0 0.0 6.664027711692837E-4 8.885370282257115E-5 22-23 6.21975919757998E-4 0.0 0.0 9.329638796369971E-4 1.3328055423385672E-4 24-25 7.552564739918548E-4 0.0 0.0 0.0013105921166329244 1.3328055423385672E-4 26-27 7.552564739918548E-4 0.0 0.0 0.002221342570564279 1.3328055423385672E-4 28-29 0.0010662444338708538 0.0 0.0 0.005819917534878411 1.3328055423385672E-4 30-31 0.0011995249881047105 0.0 0.0 0.013661256808970314 1.3328055423385672E-4 32-33 0.001243951839515996 0.0 0.0 0.029521642762799265 1.3328055423385672E-4 34-35 0.0013328055423385671 0.0 0.0 0.04962479302640599 1.777074056451423E-4 36-37 0.0015105129479837096 0.0 0.0 0.0790131552349714 1.777074056451423E-4 38-39 0.001688220353628852 0.0 0.0 0.12599455060240589 1.9992083135078509E-4 40-41 0.001688220353628852 0.0 0.0 0.17695214917115043 2.2213425705642787E-4 42-43 0.0018659277592739942 0.0 0.0 0.23701725227920853 2.2213425705642787E-4 44-45 0.002176915719152993 0.0 0.0 0.3102104899793015 2.2213425705642787E-4 46-47 0.002465690253326349 0.0 0.0 0.40190751129219493 2.2213425705642787E-4 48-49 0.0025323305304432776 0.0 0.0 0.4878512553473269 2.2213425705642787E-4 50-51 0.0025323305304432776 0.0 0.0 0.5880560187054815 2.2213425705642787E-4 52-53 0.0025323305304432776 0.0 0.0 0.6919926375821841 2.2213425705642787E-4 54-55 0.0025323305304432776 0.0 0.0 0.8111898799186633 2.2213425705642787E-4 56-57 0.00271003793608842 0.0 0.0 0.9728591922043315 2.2213425705642787E-4 58-59 0.0027544647874997055 0.0 0.0 1.1227109620145979 2.2213425705642787E-4 60-61 0.0027766782132053486 0.0 0.0 1.2893338682326243 2.2213425705642787E-4 62-63 0.002821105064616634 0.0 0.0 1.4673078349862343 2.6656110846771345E-4 64-65 0.002932172193144848 0.0 0.0 1.6508573715919606 2.6656110846771345E-4 66-67 0.0030210258959674193 0.0 0.0 1.876567990186997 2.6656110846771345E-4 68-69 0.0030654527473787047 0.0 0.0 2.1418185065380775 2.6656110846771345E-4 70-71 0.0030876661730843474 0.0 0.0 2.42026379775831 2.8877453417335626E-4 72-73 0.0031543064502012755 0.0 0.0 2.69413312328318 3.332013855846418E-4 74-75 0.003332013855846418 0.0 0.0 3.0183602848827418 3.554148112902846E-4 76-77 0.003443080984374632 0.0 0.0 3.3311253188181924 3.554148112902846E-4 78-79 0.0035097212614915605 0.0 0.0 3.7570899701495986 3.554148112902846E-4 80-81 0.004042843478426987 0.0 0.0 4.2037575342386635 3.9984166270157017E-4 82-83 0.004087270329838273 0.0 0.0 4.609330260772289 5.331222169354269E-4 84-85 0.004198337458366487 0.0 0.0 5.05719734984946 5.331222169354269E-4 86-87 0.004353831438305986 0.0 0.0 5.513860955506065 5.331222169354269E-4 88-89 0.004398258289717272 0.0 0.0 6.029301285579799 5.331222169354269E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTAAGCG 785 0.0 36.574497 94-95 TTTGCGT 1175 0.0 30.693693 3 CTTTGCG 1340 0.0 27.268377 2 TTGCGTT 1415 0.0 25.82306 4 GAGTACT 7570 0.0 21.941427 12-13 GTACTTT 7895 0.0 20.587381 14-15 TATAGGG 470 0.0 20.193218 2 AGTACTT 7835 0.0 19.624504 12-13 GTACATG 8775 0.0 19.377983 1 TACATGG 8965 0.0 18.949865 2 TCAACGA 530 0.0 17.90799 94-95 ACATGGG 9010 0.0 17.854525 3 ACTTTTT 9345 0.0 17.595522 16-17 TACTTTT 8650 0.0 17.583471 14-15 TATACGC 135 0.009455649 17.575579 3 GTCTACG 190 1.8278472E-4 17.548174 1 GTTGATC 1650 0.0 17.400595 94-95 GCTTTGC 2140 0.0 17.360744 1 AGAGTAC 14285 0.0 17.042362 10-11 CATGGGG 4580 0.0 16.68145 4 >>END_MODULE