##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938926_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2257441 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.13645938033375 33.0 31.0 34.0 30.0 34.0 2 32.36198243940816 34.0 31.0 34.0 30.0 34.0 3 32.46043285295164 34.0 31.0 34.0 30.0 34.0 4 36.007335739893094 37.0 35.0 37.0 35.0 37.0 5 35.932896142136165 37.0 35.0 37.0 35.0 37.0 6 35.99502312574282 37.0 35.0 37.0 35.0 37.0 7 35.94313384048576 37.0 35.0 37.0 35.0 37.0 8 35.925419091794645 37.0 35.0 37.0 35.0 37.0 9 37.5762179388077 39.0 37.0 39.0 35.0 39.0 10-11 37.49240179477559 39.0 37.0 39.0 34.5 39.0 12-13 37.438968504603224 39.0 37.0 39.0 34.0 39.0 14-15 38.60811135263336 40.0 38.0 41.0 34.0 41.0 16-17 38.644132670576994 40.0 38.0 41.0 34.0 41.0 18-19 38.793305782963984 40.0 38.0 41.0 34.0 41.0 20-21 38.77734722635054 40.0 38.0 41.0 34.0 41.0 22-23 38.719041826563796 40.0 38.0 41.0 34.0 41.0 24-25 38.61820530414748 40.0 38.0 41.0 34.0 41.0 26-27 38.43345894754282 40.0 38.0 41.0 34.0 41.0 28-29 38.292764240571515 40.0 38.0 41.0 34.0 41.0 30-31 38.18480770926018 40.0 38.0 41.0 33.5 41.0 32-33 38.00462625601289 40.0 37.0 41.0 33.0 41.0 34-35 37.93424944439301 40.0 37.0 41.0 33.0 41.0 36-37 37.821382928723274 40.0 37.0 41.0 33.0 41.0 38-39 37.67817099095835 40.0 37.0 41.0 33.0 41.0 40-41 37.61809478077168 40.0 37.0 41.0 33.0 41.0 42-43 37.42073591292087 40.0 37.0 41.0 32.0 41.0 44-45 37.15691727934417 40.0 36.0 41.0 31.0 41.0 46-47 36.8434539374451 40.0 36.0 41.0 31.0 41.0 48-49 36.509867367519234 39.0 36.0 41.0 30.0 41.0 50-51 36.2210128636806 39.0 35.0 41.0 30.0 41.0 52-53 36.04626743290301 39.0 35.0 40.0 29.0 41.0 54-55 35.81690130550477 39.0 35.0 40.0 28.5 41.0 56-57 35.44383640591271 38.0 34.5 40.0 28.0 41.0 58-59 35.114427353804594 38.0 34.0 40.0 27.5 41.0 60-61 34.555514186195786 37.0 34.0 40.0 26.0 41.0 62-63 34.27805865136675 37.0 34.0 40.0 26.0 41.0 64-65 34.01734552530941 36.5 33.5 39.0 26.0 40.5 66-67 33.46640177971429 36.0 33.0 39.0 25.5 40.0 68-69 32.97706451685781 35.0 32.0 38.0 24.5 40.0 70-71 32.40340323401586 35.0 32.0 37.5 23.5 39.0 72-73 31.854914037620475 35.0 31.0 37.0 21.5 39.0 74-75 31.433988750979537 34.5 31.0 36.0 20.5 38.5 76-77 29.50162041887252 32.5 28.5 34.5 18.0 36.0 78-79 30.49596556454853 34.0 30.0 35.0 19.0 37.0 80-81 30.46579711274846 34.0 30.5 35.0 18.0 36.5 82-83 30.144872889258238 34.0 30.0 35.0 17.0 36.0 84-85 29.746287499872643 34.0 30.0 35.0 11.5 36.0 86-87 29.29367921465057 34.0 29.0 35.0 7.0 35.0 88-89 28.708655065625194 34.0 29.0 35.0 2.0 35.0 90-91 28.21812751695393 33.0 28.0 35.0 2.0 35.0 92-93 27.878830277291854 33.0 27.0 35.0 2.0 35.0 94-95 27.450094155284678 33.0 27.0 35.0 2.0 35.0 96-97 26.77590001244772 33.0 25.0 34.0 2.0 35.0 98-99 25.8834640639556 32.0 24.5 34.0 2.0 35.0 100-101 21.412605246382963 27.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.22803572125771865 1101 2 0.21602735723763544 1101 3 0.20251139261731055 1101 4 0.15266002716690963 1101 5 0.17353506991054246 1101 6 0.17886206735791177 1101 7 0.14313169940088954 1101 8 0.2245754936088744 1101 9 0.2596795369883509 1101 10-11 0.23496563901081657 1101 12-13 0.25973970863814344 1101 14-15 0.3205399596310272 1101 16-17 0.35758725958726245 1101 18-19 0.44317235346753847 1101 20-21 0.4256739803982228 1101 22-23 0.41085455528686055 1101 24-25 0.34783520969972415 1101 26-27 0.3128301532785045 1101 28-29 0.36507023271634154 1101 30-31 0.27784772120107704 1101 32-33 0.45940797706812475 1101 34-35 0.5452602352394536 1101 36-37 0.524394180150118 1101 38-39 0.4946866623504036 1101 40-41 0.4713687702399909 1101 42-43 0.41955674879122995 1101 44-45 0.45047828474984897 1101 46-47 0.4290150172872771 1101 48-49 0.3009453417640273 1101 50-51 0.340627351983251 1101 52-53 0.3129155821899019 1101 54-55 0.37229948475356167 1101 56-57 0.21862459461301853 1101 58-59 0.3525193575366714 1101 60-61 0.4776425020757884 1101 62-63 0.3976494030699982 1101 64-65 0.4231710837888727 1101 66-67 0.3776955844192784 1101 68-69 0.5021596587955628 1101 70-71 0.5025560877061679 1101 72-73 0.4428491365497038 1101 74-75 0.41866314021521234 1101 76-77 0.1947425651158916 1101 78-79 0.5724173850687322 1101 80-81 0.7715805317998949 1101 82-83 0.7674878917461179 1101 84-85 0.6103994929389422 1101 86-87 0.7853557309035075 1101 88-89 0.8303614177407894 1101 90-91 0.9033712971897039 1101 92-93 0.9555111804413734 1101 94-95 0.7824350304546464 1101 96-97 0.7787972469473594 1101 98-99 0.8889795088326693 1101 100-101 1.4425782639033642 1102 1 0.08070330175548435 1102 2 0.06396630973633677 1102 3 0.15417823846654954 1102 4 0.12980484251927038 1102 5 0.11426371582786743 1102 6 0.11109521971685155 1102 7 0.12830658158835462 1102 8 0.1918899052629115 1102 9 0.27099446827183726 1102 10-11 0.1972486041239705 1102 12-13 0.20905343939841003 1102 14-15 0.2833331471097509 1102 16-17 0.2262450925532704 1102 18-19 0.2320215982130165 1102 20-21 0.18386687939301538 1102 22-23 0.26874284391414704 1102 24-25 0.23185705205490592 1102 26-27 0.23719696687486902 1102 28-29 0.21072012789453964 1102 30-31 0.2396746666758176 1102 32-33 0.19482923528812535 1102 34-35 0.3847433383231902 1102 36-37 0.3041859700048022 1102 38-39 0.2890177738198787 1102 40-41 0.28061974230542575 1102 42-43 0.19088149032037194 1102 44-45 0.15475134106692678 1102 46-47 0.3563434947729931 1102 48-49 0.2891627439751332 1102 50-51 0.24557749443339816 1102 52-53 0.22660221875389652 1102 54-55 0.35737447265873357 1102 56-57 0.4601992062348046 1102 58-59 0.3105695824107926 1102 60-61 0.10555085915081719 1102 62-63 0.30665489501574683 1102 64-65 0.31293483157148927 1102 66-67 0.3679305823586745 1102 68-69 0.4007367252185432 1102 70-71 0.4070296045224495 1102 72-73 0.47235826590160457 1102 74-75 0.515744032186042 1102 76-77 0.2366085723907041 1102 78-79 0.3354663556648738 1102 80-81 0.5620540665344365 1102 82-83 0.6678124361744402 1102 84-85 0.5685690981997418 1102 86-87 0.48976832564628836 1102 88-89 0.7240425444407848 1102 90-91 0.7991231024540859 1102 92-93 0.6716535141151532 1102 94-95 0.7090239318863105 1102 96-97 0.41391725447303074 1102 98-99 0.6949639826630545 1102 100-101 1.072045509329726 1103 1 0.01781745664614931 1103 2 0.018490532783097535 1103 3 0.018677199020217472 1103 4 -0.030456948837098707 1103 5 0.06552925269970444 1103 6 -0.020379390600417935 1103 7 0.08706753524747057 1103 8 0.08539753225518609 1103 9 0.02136660760150022 1103 10-11 0.08683835176218935 1103 12-13 0.01799267783950853 1103 14-15 0.12063194626416163 1103 16-17 0.11052467291519008 1103 18-19 0.08115528329147281 1103 20-21 0.0792936444781347 1103 22-23 0.14431881559910664 1103 24-25 0.15671758204095454 1103 26-27 -0.0036972420494478797 1103 28-29 0.15621388683070592 1103 30-31 0.0931592240997503 1103 32-33 0.12272696604959776 1103 34-35 0.10920683928368646 1103 36-37 -0.0077623997749398654 1103 38-39 0.22634595816590775 1103 40-41 0.1419552992644526 1103 42-43 0.18756765208804893 1103 44-45 0.20861900244425868 1103 46-47 0.3124790789242837 1103 48-49 0.19796799471415483 1103 50-51 0.21122719224836572 1103 52-53 0.38212608654684743 1103 54-55 0.3234402779445631 1103 56-57 0.28687251746345765 1103 58-59 0.15457127534870096 1103 60-61 0.14585188193091625 1103 62-63 0.14156000716191386 1103 64-65 0.1244549231480292 1103 66-67 0.3650409654187712 1103 68-69 0.3545290596307993 1103 70-71 0.31179011521076205 1103 72-73 0.2848884186168803 1103 74-75 0.2672557248607994 1103 76-77 0.08854667928262572 1103 78-79 0.27474426940398544 1103 80-81 0.3327705169787194 1103 82-83 0.34708414393651665 1103 84-85 0.2606744594621624 1103 86-87 0.1566767622663754 1103 88-89 0.43496988845804907 1103 90-91 0.5260790816827026 1103 92-93 0.5220077811081296 1103 94-95 0.33809290375547363 1103 96-97 0.13817988352665012 1103 98-99 0.23627375082704916 1103 100-101 0.7471434234197076 1104 1 0.047012833915125896 1104 2 0.03085099795966073 1104 3 0.0881510237650005 1104 4 0.09361735567070184 1104 5 0.04786650743900367 1104 6 0.10694813407471315 1104 7 0.09773388045908149 1104 8 0.14486563648266326 1104 9 0.07033514302809607 1104 10-11 0.04125330356985302 1104 12-13 0.13115749354724926 1104 14-15 0.10982539653108603 1104 16-17 0.09552246507203677 1104 18-19 0.17181608513171653 1104 20-21 0.09491759132399125 1104 22-23 0.0809377306364567 1104 24-25 0.06712656878353584 1104 26-27 0.11133160249246288 1104 28-29 0.056545862621341314 1104 30-31 -0.0027097760540684135 1104 32-33 0.09895527863525189 1104 34-35 0.1804963017797263 1104 36-37 0.10824625618001704 1104 38-39 0.2735359052640831 1104 40-41 0.2603077902818569 1104 42-43 0.27741617503664884 1104 44-45 0.264196701240877 1104 46-47 0.2854300838169692 1104 48-49 0.23502530271821342 1104 50-51 0.08856076580885741 1104 52-53 0.08753456777543533 1104 54-55 0.14282898623101659 1104 56-57 0.022340610457291632 1104 58-59 0.054061375915736676 1104 60-61 0.28724230896494163 1104 62-63 0.2791289428518908 1104 64-65 0.12878581705230374 1104 66-67 0.16575708863629046 1104 68-69 0.14665101293685012 1104 70-71 0.1567069559989136 1104 72-73 0.099637847861338 1104 74-75 0.2271910406731017 1104 76-77 0.08012403847753902 1104 78-79 0.14124158888364136 1104 80-81 0.163474421447809 1104 82-83 0.29154025408844575 1104 84-85 0.2248677585069565 1104 86-87 0.09232321096578033 1104 88-89 0.2831556117059897 1104 90-91 0.27872698125046824 1104 92-93 0.3654355159388203 1104 94-95 0.3974094618014412 1104 96-97 0.35357496647414877 1104 98-99 0.5723838465895454 1104 100-101 1.1840694274050563 1105 1 -0.1655273937210069 1105 2 0.016124478417630428 1105 3 -0.07347046200943197 1105 4 -0.04523967345511437 1105 5 0.00539219577856187 1105 6 0.0070562511177385545 1105 7 0.009735715581435045 1105 8 -0.031801153874845056 1105 9 -0.14235604366187005 1105 10-11 -0.015588261951428706 1105 12-13 -0.05817509876548144 1105 14-15 -0.027758179123857474 1105 16-17 0.00476613491193234 1105 18-19 0.0046618235323236945 1105 20-21 -0.02574043834832196 1105 22-23 -0.11293754851129023 1105 24-25 -0.03198142344418642 1105 26-27 -0.11044411452087388 1105 28-29 -0.0746101463923452 1105 30-31 -0.04769135736280106 1105 32-33 -0.1555414458062856 1105 34-35 -0.09406406160147895 1105 36-37 0.04168554196658647 1105 38-39 0.040228265892608306 1105 40-41 0.07449237525409558 1105 42-43 -0.0024218518020120428 1105 44-45 0.03596474379952497 1105 46-47 -0.014018283126539188 1105 48-49 -0.2439066324587884 1105 50-51 -0.23788439304392028 1105 52-53 0.01305609335582858 1105 54-55 -0.09548054223699154 1105 56-57 -0.1518530657920394 1105 58-59 -0.26546370184082235 1105 60-61 -0.2806787234263126 1105 62-63 -0.39879274734976633 1105 64-65 -0.38123271970749073 1105 66-67 -0.17693809551688133 1105 68-69 -0.19390038398256593 1105 70-71 -0.210063245527202 1105 72-73 -0.28835981100830566 1105 74-75 -0.15589201548029408 1105 76-77 -0.2815316871716682 1105 78-79 -0.21109136100806936 1105 80-81 -0.21792615810002047 1105 82-83 -0.14706916845522144 1105 84-85 -0.2533768491344368 1105 86-87 -0.08806400322403363 1105 88-89 -0.0660868943155073 1105 90-91 -0.05377171999566244 1105 92-93 0.1966873291341571 1105 94-95 0.14295195298003804 1105 96-97 -0.0425816497491418 1105 98-99 0.14978240885214333 1105 100-101 0.5518314046144575 1106 1 0.02375458688126031 1106 2 -0.04951423566059532 1106 3 -0.06330242683548448 1106 4 0.03282538055059803 1106 5 -0.018234635476957806 1106 6 -0.05830071550013116 1106 7 0.02016181330496636 1106 8 0.009918065832977163 1106 9 0.011278086665278408 1106 10-11 0.050002567415880605 1106 12-13 0.11131793292781822 1106 14-15 0.058309594321521274 1106 16-17 0.07089368495299908 1106 18-19 0.01434019892045768 1106 20-21 0.08423333936611499 1106 22-23 0.18104285933051756 1106 24-25 0.13783753321695258 1106 26-27 0.14187144593002188 1106 28-29 0.24620454451338247 1106 30-31 0.15937593167466702 1106 32-33 0.04481189187134049 1106 34-35 0.03275473359035885 1106 36-37 -0.09204172978707703 1106 38-39 0.10344257190824635 1106 40-41 0.16856317707276958 1106 42-43 0.3148527687657179 1106 44-45 0.21264388040008697 1106 46-47 0.06559267470772312 1106 48-49 -0.19111008295897847 1106 50-51 -0.030426817868011824 1106 52-53 0.1252176359808317 1106 54-55 0.08144028343135545 1106 56-57 0.0496149043822669 1106 58-59 0.11827541236822725 1106 60-61 0.24487791316806806 1106 62-63 0.09208845902365681 1106 64-65 0.06028985345417226 1106 66-67 0.019982886677830436 1106 68-69 -0.05508734401809079 1106 70-71 0.004061632032104967 1106 72-73 0.07913269014298407 1106 74-75 0.16879979234157716 1106 76-77 0.06152570939941526 1106 78-79 0.1610429707437966 1106 80-81 0.25496285729146706 1106 82-83 0.12643800251193582 1106 84-85 0.18036893804078247 1106 86-87 0.10541947459412171 1106 88-89 0.40404622724039996 1106 90-91 0.5303053881879514 1106 92-93 0.46228796199497424 1106 94-95 0.4816517378798544 1106 96-97 0.36358676603102325 1106 98-99 0.7950863501036807 1106 100-101 1.1396842994872287 1107 1 -0.03692922295250867 1107 2 -0.005777625573635703 1107 3 -0.06889004545079302 1107 4 -0.1272257055490158 1107 5 -0.09204805416314343 1107 6 -0.05422373805493663 1107 7 -0.05239871939996732 1107 8 -0.10501036427886845 1107 9 0.0566413685413778 1107 10-11 0.008030275282997934 1107 12-13 0.039915973091133594 1107 14-15 0.08461347055988 1107 16-17 0.08363502966018643 1107 18-19 0.020128067215708256 1107 20-21 -0.044154234713012386 1107 22-23 -0.0028991147561185926 1107 24-25 -0.04139092638175157 1107 26-27 -0.00587356322395749 1107 28-29 -0.0180534570111206 1107 30-31 -0.035437642398754576 1107 32-33 0.014348552448957719 1107 34-35 -0.20587885394994743 1107 36-37 -0.07074664455045365 1107 38-39 -0.022100824562869548 1107 40-41 -0.1311390178023899 1107 42-43 -0.15652417005698283 1107 44-45 -0.17694227977764143 1107 46-47 -0.2952063704561212 1107 48-49 -0.2582092236798488 1107 50-51 -0.2720887229969762 1107 52-53 -0.25649526552282964 1107 54-55 -0.25232903138439156 1107 56-57 -0.2472468854093961 1107 58-59 -0.045023409502952916 1107 60-61 -0.17920879942959544 1107 62-63 -0.22406163688003033 1107 64-65 -0.29917208666750383 1107 66-67 -0.11290948951913293 1107 68-69 -0.15670936642566602 1107 70-71 -0.2270337377956082 1107 72-73 -0.19464532798878764 1107 74-75 -0.34862029845187337 1107 76-77 -0.6856735480046261 1107 78-79 -0.3810655401123064 1107 80-81 -0.09013596041974381 1107 82-83 -0.06813769859644836 1107 84-85 -0.3016353043159832 1107 86-87 -0.35732286932226387 1107 88-89 -0.09841927047385823 1107 90-91 0.032085815841330856 1107 92-93 -0.060994733863282136 1107 94-95 -0.035585232534170785 1107 96-97 -0.030837911744402646 1107 98-99 -0.15336261212580382 1107 100-101 0.548965994504929 1108 1 -0.039353076677706156 1108 2 -0.0675945625043326 1108 3 -0.03681226256914982 1108 4 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-1.2957206270315282 2316 54-55 -1.3944881655381138 2316 56-57 -1.4199537592177052 2316 58-59 -1.3654828530283325 2316 60-61 -1.2635511891300908 2316 62-63 -1.3260011414778887 2316 64-65 -1.3502675396863282 2316 66-67 -1.4971537505007753 2316 68-69 -1.5849820709333429 2316 70-71 -1.58060669449344 2316 72-73 -1.599102100414946 2316 74-75 -1.7990551079224453 2316 76-77 -1.9316361723333166 2316 78-79 -2.1142824400729374 2316 80-81 -1.9841973654678853 2316 82-83 -2.114603092941792 2316 84-85 -2.29315140945371 2316 86-87 -2.3939798282784324 2316 88-89 -2.5720237624424875 2316 90-91 -2.6895035210569738 2316 92-93 -3.047878479922197 2316 94-95 -3.200311342989121 2316 96-97 -3.2277245103059258 2316 98-99 -3.524112531118675 2316 100-101 -4.061797536314916 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 5.0 4 16.0 5 40.0 6 92.0 7 270.0 8 653.0 9 1179.0 10 1965.0 11 3084.0 12 4427.0 13 6015.0 14 7566.0 15 10107.0 16 13197.0 17 18542.0 18 18535.0 19 13759.0 20 12896.0 21 13940.0 22 15229.0 23 16436.0 24 18641.0 25 22362.0 26 26666.0 27 32797.0 28 39068.0 29 47550.0 30 58720.0 31 70757.0 32 87666.0 33 112402.0 34 152306.0 35 222577.0 36 356973.0 37 519450.0 38 313348.0 39 18203.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.67493902924506 19.07469456798723 15.457014894967505 20.79335150780021 2 15.826194350151344 22.908682884735416 35.46998570505276 25.795137060060487 3 22.9278195975 23.68101757698208 30.195517845206144 23.195644980311776 4 13.169247834162665 16.90981071044603 38.11873710099179 31.802204354399517 5 13.396986754424162 38.42302296457968 33.42473508626834 14.755255194727818 6 30.601818607883885 36.36086170136894 15.85055822056922 17.18676147017796 7 27.056166694943524 33.36299819131486 20.654227508050045 18.92660760569158 8 26.434666509556614 30.040386437563594 19.367948043824846 24.156999009054942 9 24.343759150294513 19.065658858858328 22.231322989172252 34.35925900167491 10-11 26.131247726961636 24.944483598906906 26.07616323084413 22.848105443287334 12-13 29.01570849470706 23.144591597299772 25.826699346738184 22.013000561254977 14-15 23.80333572471644 25.130608721807572 27.56855698155032 23.497498571925664 16-17 21.631150692747546 28.05501313907933 27.256501762213674 23.057334405959455 18-19 21.462089153160594 29.03433578109018 27.54696579002508 21.956609275724148 20-21 24.00970833789233 25.185730213990087 26.57086054519254 24.233700902925037 22-23 22.63069112326745 25.330495902218487 28.10142103381661 23.937391940697452 24-25 24.90118235648241 25.655997211001306 27.043010204917866 22.39981022759842 26-27 23.477424216181063 24.009752635838545 30.460375265621558 22.05244788235883 28-29 21.752639382380316 28.54453338979845 27.678929371797533 22.0238978560237 30-31 22.90591426309702 25.262587150671934 27.129524093874437 24.70197449235661 32-33 23.267518398044512 25.611854307598737 28.235798853657744 22.884828440699003 34-35 25.064167252153158 25.10536541887904 27.404422468151157 22.426044860816646 36-37 23.68698576050131 24.38895970920722 29.898229172088943 22.025825358202525 38-39 22.746544954796658 26.298261423557484 28.75480587328887 22.200387748356988 40-41 22.82482732224557 26.850194738688675 27.575060335326203 22.74991760373955 42-43 22.302094534806844 26.89656012554141 25.84722177616286 24.95412356348889 44-45 23.61289811008662 24.478483621762166 26.9926019903594 24.916016277791815 46-47 25.29398371073757 26.541747768930975 27.05970219337142 21.104566326960033 48-49 24.085526366790592 25.841193479760744 27.34834817873846 22.72493197471021 50-51 20.977296759015005 26.750850856571027 29.55667305002899 22.71517933438497 52-53 23.281560846994452 28.11825425337805 25.25848959064711 23.34169530898039 54-55 22.281667605044827 25.608908494175488 26.864533779620377 25.244890121159315 56-57 24.218639601212168 25.106879869728598 26.539519748245908 24.13496078081332 58-59 26.25127633796765 25.13485438118927 25.97328383762988 22.640585443213205 60-61 23.513197014865206 26.191472391401472 28.83180564418439 21.463524949548937 62-63 22.063610964804838 26.560317633993535 28.202065967615543 23.174005433586085 64-65 21.90642392519655 28.58705628863897 26.499754588834413 23.00676519733007 66-67 23.381686083148185 25.836874090077384 25.46243947969426 25.319000347080173 68-69 22.243003281433598 26.0476943028421 27.06762987023774 24.64167254548656 70-71 24.83759265469175 25.96869641332819 25.753784927269418 23.439926004710642 72-73 23.391596945390823 24.537141834493127 28.888817027776142 23.18244419233991 74-75 21.83120178999141 29.001909684461296 26.079241938106023 23.087646587441267 76-77 22.90761198604269 25.727172446951528 25.977528498542306 25.387687068463478 78-79 23.106083090654096 26.107915256144352 27.04468516964807 23.74131648355348 80-81 24.686647402966454 25.71710622780396 26.124691630921916 23.471554738307667 82-83 23.271372319365156 25.115894501783213 28.4772448094989 23.135488369352732 84-85 22.492326488267025 26.69682175525296 27.296660244941062 23.51419151153895 86-87 22.50919570673293 27.085487797249474 26.546966093340806 23.85835040267679 88-89 22.14929434829 26.953924178148693 25.119425511674244 25.777355961887068 90-91 23.127833438120124 24.89683001627029 26.21040965078552 25.764926894824065 92-93 24.371578260517108 26.487026681981945 26.500648300442847 22.640746757058103 94-95 23.244948594448317 25.74200167357641 26.898443857447436 24.11460587452784 96-97 20.522634840462175 26.268291289153535 29.049422196155177 24.15965167422911 98-99 22.428980425180548 27.35588659902961 25.380220346844055 24.834912628945784 100-101 21.91989325952695 24.889653669336656 27.050731847341986 26.13972122379441 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 758.0 1 533.5 2 339.5 3 387.5 4 367.5 5 419.0 6 686.0 7 1346.5 8 2294.5 9 2876.0 10 2642.0 11 2292.0 12 2306.0 13 2416.0 14 2636.5 15 2788.5 16 2914.0 17 3053.5 18 3215.5 19 3492.0 20 3814.0 21 4314.5 22 4975.0 23 5741.5 24 6582.0 25 7771.5 26 9380.5 27 10975.5 28 12728.0 29 14823.5 30 17087.5 31 19994.0 32 22734.5 33 24945.0 34 27489.0 35 30108.0 36 34054.5 37 37678.0 38 39994.5 39 42176.0 40 43474.0 41 44873.5 42 46832.5 43 50531.0 44 55500.5 45 60678.5 46 70561.5 47 123487.0 48 212531.0 49 204677.0 50 122457.5 51 84042.5 52 75748.5 53 75086.5 54 75274.0 55 72847.0 56 67783.0 57 61422.0 58 56542.0 59 51180.5 60 43530.0 61 35995.0 62 28853.0 63 22256.5 64 16419.0 65 11926.5 66 8534.5 67 6296.0 68 4561.0 69 3106.0 70 2126.5 71 1297.5 72 850.5 73 594.5 74 355.5 75 204.5 76 116.5 77 50.5 78 25.0 79 20.5 80 12.0 81 5.5 82 5.0 83 5.5 84 4.5 85 2.0 86 0.5 87 0.5 88 1.5 89 1.5 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4089586394505991 2 0.0 3 0.0 4 0.0 5 3.543835697145573E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0013510873595367497 16-17 0.0023477911493589424 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0026135788266448603 36-37 0.007242714206041266 38-39 0.013599469487796136 40-41 0.0025249829342162207 42-43 8.195120049649138E-4 44-45 4.2083048903603684E-4 46-47 1.3289383864295898E-4 48-49 3.7653254282171716E-4 50-51 4.429794621431967E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 2.6578767728591796E-4 60-61 0.0024585360148947416 62-63 0.0 64-65 2.2148973107159834E-4 66-67 0.0016611729830369876 68-69 0.0019269606603229054 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.12633774260323968 78-79 1.3289383864295898E-4 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.15061301712868685 88-89 0.44364393133641145 90-91 0.3381926703732235 92-93 0.0 94-95 0.0 96-97 6.201712470004754E-4 98-99 0.0 100-101 1.3289383864295898E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2257441.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.46766594535178 #Duplication Level Percentage of deduplicated Percentage of total 1 70.1491221437364 28.387712412724042 2 14.838112161257246 12.009275324028401 3 5.683509658778188 6.899951108058482 4 2.7895742146916342 4.515502297996323 5 1.6498640232154065 3.3383073073367595 6 1.0477733773931823 2.544056581366818 7 0.7366924272303373 2.0868556134739373 8 0.5455167010572399 1.7660630100795776 9 0.3994724370872272 1.4549145424579322 >10 2.0124277122138223 14.448980962889118 >50 0.10264179810841077 2.819272534441393 >100 0.03849535812453378 2.7803679067306053 >500 0.002302544019874061 0.6326825732787631 >1k 0.001973609159892052 1.8884636370935854 >5k 0.0012060944866006988 3.317423751578652 >10k+ 0.001315739439928035 11.110170436465571 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 58620 2.596745607083419 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 32190 1.42595088863895 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 23436 1.0381666674787957 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 18751 0.8306307894647081 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 17728 0.7853139904874591 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 17202 0.7620132707787269 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 16402 0.7265749138072711 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 14793 0.6552995183484308 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 14767 0.6541477717468585 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 13170 0.5834039516425901 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 12443 0.5511993447447796 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 10892 0.4824932301663698 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8504 0.3767097346065744 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 8387 0.37152687489949904 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 7717 0.34184725093590485 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 7626 0.33781613783040176 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7332 0.3247925416433918 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 7182 0.3181478497112438 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 5962 0.26410435532977383 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 5720 0.2533842523459085 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 5714 0.25311846466862253 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 5475 0.24253125552340019 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 5147 0.2280015291651033 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4348 0.1926074701398619 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 3897 0.17262909639720372 No Hit GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 3697 0.1637695071543398 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 3559 0.1576563905767637 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 3204 0.1419306196706802 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 2636 0.11676938622094664 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 2628 0.11641500265123209 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 2622 0.11614921497394616 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 2409 0.10671375243029607 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 2287 0.10130940299214908 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 4.4297946214319665E-5 0.0 0.0 0.0 0.0 3 4.4297946214319665E-5 0.0 0.0 0.0 0.0 4 4.4297946214319665E-5 0.0 0.0 0.0 0.0 5 4.4297946214319665E-5 0.0 0.0 0.0 0.0 6 4.4297946214319665E-5 0.0 0.0 0.0 0.0 7 2.2148973107159834E-4 0.0 0.0 0.0 0.0 8 2.2148973107159834E-4 0.0 0.0 0.0 0.0 9 2.2148973107159834E-4 0.0 0.0 0.0 0.0 10-11 2.2148973107159834E-4 0.0 0.0 6.644691932147949E-5 0.0 12-13 2.8793665039307786E-4 0.0 0.0 1.32893838642959E-4 4.4297946214319665E-5 14-15 5.31575354571836E-4 0.0 0.0 2.65787677285918E-4 4.4297946214319665E-5 16-17 5.31575354571836E-4 0.0 0.0 3.98681515928877E-4 4.4297946214319665E-5 18-19 5.537243276789958E-4 0.0 0.0 4.8727740835751636E-4 8.859589242863933E-5 20-21 5.758733007861556E-4 0.0 0.0 8.195120049649138E-4 1.32893838642959E-4 22-23 7.087671394291146E-4 0.0 0.0 0.0013732363326439097 1.32893838642959E-4 24-25 9.745548167150326E-4 0.0 0.0 0.0018162157947871063 1.32893838642959E-4 26-27 9.967037898221925E-4 0.0 0.0 0.0026135788266448603 1.32893838642959E-4 28-29 0.0011074486553579916 0.0 0.0 0.006002371712040315 1.32893838642959E-4 30-31 0.001196044547786631 0.0 0.0 0.012735659536616904 1.5504281175011882E-4 32-33 0.001196044547786631 0.0 0.0 0.02761976946462831 1.7719178485727866E-4 34-35 0.0012624914671081106 0.0 0.0 0.05072114841539602 1.7719178485727866E-4 36-37 0.0014175342788582293 0.0 0.0 0.08223913714688447 1.7719178485727866E-4 38-39 0.0015282791443940284 0.0 0.0 0.1336690527017096 1.7719178485727866E-4 40-41 0.0015504281175011882 0.0 0.0 0.18844346319571587 1.7719178485727866E-4 42-43 0.001727619902358467 0.0 0.0 0.24966322486390563 1.7719178485727866E-4 44-45 0.0019048116872157456 0.0 0.0 0.3306841684898963 1.7719178485727866E-4 46-47 0.002259195256930303 0.0 0.0 0.4284275868117926 1.7719178485727866E-4 48-49 0.0023477911493589424 0.0 0.0 0.5178872891916112 1.7719178485727866E-4 50-51 0.0023477911493589424 0.0 0.0 0.6175798171469375 2.2148973107159834E-4 52-53 0.0023477911493589424 0.0 0.0 0.723917037034412 2.65787677285918E-4 54-55 0.0023477911493589424 0.0 0.0 0.8445624935491116 2.65787677285918E-4 56-57 0.0025692808804305407 0.0 0.0 1.0097716839554167 2.65787677285918E-4 58-59 0.0025692808804305407 0.0 0.0 1.1653017731138933 2.65787677285918E-4 60-61 0.0025914298535377007 0.0 0.0 1.3362253985818455 2.65787677285918E-4 62-63 0.0026135788266448603 0.0 0.0 1.519198065420093 3.1008562350023764E-4 64-65 0.00265787677285918 0.0 0.0 1.7108974276625613 3.1008562350023764E-4 66-67 0.002879366503930778 0.0 0.0 1.942154855874417 3.1008562350023764E-4 68-69 0.0029679623963594177 0.0 0.0 2.211397772965052 3.1008562350023764E-4 70-71 0.003078707261895217 0.0 0.0 2.4932656047267683 3.322345966073975E-4 72-73 0.0032116011005381756 0.0 0.0 2.7700391726738376 3.543835697145573E-4 74-75 0.0032558990467524956 0.0 0.0 3.088054128546438 3.543835697145573E-4 76-77 0.003278048019859655 0.0 0.0 3.403832038135216 3.543835697145573E-4 78-79 0.003278048019859655 0.0 0.0 3.834518820203939 3.543835697145573E-4 80-81 0.003853921320645811 0.0 0.0 4.279912520415816 3.543835697145573E-4 82-83 0.004031113105503089 0.0 0.0 4.690222247225952 3.543835697145573E-4 84-85 0.004075411051717409 0.0 0.0 5.139381272866046 3.543835697145573E-4 86-87 0.004119708997931729 0.0 0.0 5.599171805597577 3.543835697145573E-4 88-89 0.004119708997931729 0.0 0.0 6.125586449435445 3.543835697145573E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTTGCGT 1325 0.0 34.02072 3 GTAAGCG 435 0.0 30.5425 94-95 CTTTGCG 1505 0.0 29.9518 2 TTGCGTT 1730 0.0 26.056334 4 TTACGTA 120 1.7238037E-4 23.724976 4 GCTTTGC 2080 0.0 22.45071 1 GAGTACT 7310 0.0 21.29317 12-13 GTACATG 8330 0.0 21.222485 1 TACATGG 8350 0.0 20.457464 2 GTACTTT 7705 0.0 19.955208 14-15 AGTACTT 7545 0.0 19.906658 12-13 ACATGGG 8760 0.0 18.958317 3 GTATTAG 410 1.8189894E-12 18.59531 1 AGAGTAC 13450 0.0 18.346994 10-11 CATGGGG 4620 0.0 17.45994 4 ACTTTTT 8860 0.0 17.406227 16-17 TACTTTT 8400 0.0 17.400242 14-15 ATATAGG 455 9.094947E-12 16.685698 2 TATACAG 770 0.0 16.022062 5 AGTATCG 180 0.0026381435 15.816651 5 >>END_MODULE