##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938925_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 509859 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.874628083450524 33.0 31.0 34.0 30.0 34.0 2 31.94060710902426 33.0 31.0 34.0 30.0 34.0 3 31.972211925257767 34.0 31.0 34.0 30.0 34.0 4 35.47823417846895 37.0 35.0 37.0 33.0 37.0 5 35.5274575912164 37.0 35.0 37.0 33.0 37.0 6 35.65328257420189 37.0 35.0 37.0 33.0 37.0 7 35.619943945286835 37.0 35.0 37.0 33.0 37.0 8 35.62356651544839 37.0 35.0 37.0 33.0 37.0 9 37.222159459772215 39.0 37.0 39.0 34.0 39.0 10-11 37.12146299270975 39.0 37.0 39.0 33.5 39.0 12-13 37.07549930470974 39.0 37.0 39.0 33.5 39.0 14-15 38.19933354123395 40.0 38.0 41.0 33.0 41.0 16-17 38.16171235576895 40.0 38.0 41.0 33.0 41.0 18-19 38.14619630133036 40.0 38.0 41.0 33.5 41.0 20-21 38.23717243394742 40.0 38.0 41.0 34.0 41.0 22-23 38.16932524482259 40.0 38.0 41.0 34.0 41.0 24-25 38.062664383682545 40.0 38.0 41.0 33.5 41.0 26-27 37.90962403331117 40.0 38.0 41.0 33.0 41.0 28-29 37.81251581319541 40.0 37.5 41.0 33.0 41.0 30-31 37.65246470102518 40.0 37.0 41.0 33.0 41.0 32-33 37.3984562398624 40.0 36.5 41.0 33.0 41.0 34-35 37.05372759919899 40.0 36.0 41.0 31.5 41.0 36-37 36.746182768177086 39.5 35.5 41.0 30.5 41.0 38-39 36.498444668035674 39.0 35.0 41.0 30.0 41.0 40-41 36.44703633749723 39.0 35.0 41.0 30.0 41.0 42-43 36.231415940485505 39.0 35.0 41.0 30.0 41.0 44-45 36.08832245777754 39.0 35.0 41.0 30.0 41.0 46-47 35.79810888892811 39.0 35.0 41.0 28.0 41.0 48-49 35.519992782318255 39.0 35.0 41.0 27.0 41.0 50-51 34.39816106021469 38.0 34.0 40.0 24.5 40.5 52-53 34.577570465560086 38.5 34.5 39.5 24.0 40.5 54-55 35.14863913356438 39.0 35.0 40.5 25.0 41.0 56-57 35.07710072784829 39.0 35.0 41.0 24.5 41.0 58-59 34.92747602768608 39.0 35.0 41.0 24.0 41.0 60-61 34.57209640312321 39.0 35.0 40.5 22.0 41.0 62-63 34.27149368747045 38.0 34.0 40.0 22.0 41.0 64-65 33.91127743160364 38.0 34.0 40.0 21.0 41.0 66-67 33.43239993802209 37.0 33.5 39.5 19.0 41.0 68-69 32.98047793605683 37.0 33.0 39.0 16.0 41.0 70-71 32.43244799052287 36.0 32.5 39.0 10.5 40.0 72-73 31.93838002271216 35.5 32.0 38.0 7.5 40.0 74-75 31.456993011793458 35.0 31.0 37.0 4.5 39.5 76-77 31.012270058977087 35.0 31.0 37.0 2.0 39.0 78-79 30.551687819573644 35.0 31.0 36.0 2.0 39.0 80-81 30.032980686817336 35.0 30.5 36.0 2.0 38.5 82-83 29.500203977962535 34.0 29.5 35.5 2.0 37.0 84-85 29.013825390941417 34.0 29.0 35.0 2.0 37.0 86-87 28.630144804740134 34.0 29.0 35.0 2.0 36.0 88-89 28.07583567221526 34.0 28.5 35.0 2.0 36.0 90-91 27.56460511631647 34.0 27.0 35.0 2.0 35.5 92-93 27.065524978474443 34.0 26.0 35.0 2.0 35.0 94-95 26.504361009612463 33.5 25.0 35.0 2.0 35.0 96-97 25.838819163729582 33.0 23.5 35.0 2.0 35.0 98-99 25.110340309771917 33.0 10.5 35.0 2.0 35.0 100-101 22.05715697869411 28.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.276790193189413 1101 2 0.5337379629841443 1101 3 0.6959619325869291 1101 4 0.28488065484290814 1101 5 0.5097042513600698 1101 6 0.5183222790126081 1101 7 0.47113936445269644 1101 8 0.5873745439368889 1101 9 0.5977456213558554 1101 10-11 0.5486529758376335 1101 12-13 0.5038749564517033 1101 14-15 0.7165885582739193 1101 16-17 0.6965548475413996 1101 18-19 0.7070568654946925 1101 20-21 0.5534768367809448 1101 22-23 0.571480927668766 1101 24-25 0.6647219287273387 1101 26-27 0.6904696300575779 1101 28-29 0.7472692539813934 1101 30-31 0.5434897615302887 1101 32-33 0.5596225158884565 1101 34-35 0.6612021189889958 1101 36-37 0.9087048526045365 1101 38-39 0.8837162406979928 1101 40-41 0.5600947332655082 1101 42-43 0.7098218624331594 1101 44-45 0.7516086225677157 1101 46-47 0.6828985119274904 1101 48-49 0.5016094716658444 1101 50-51 0.49304904136791805 1101 52-53 0.43128105871360845 1101 54-55 0.33093046383584124 1101 56-57 0.5264467996412918 1101 58-59 0.39447048442118415 1101 60-61 0.49268527701678266 1101 62-63 0.19007943410516503 1101 64-65 0.34503884378933947 1101 66-67 0.36430332448563263 1101 68-69 0.4391723331244677 1101 70-71 0.22914270740410103 1101 72-73 0.09319061125486527 1101 74-75 -0.06289594972173873 1101 76-77 -0.09044774525696297 1101 78-79 -0.02101369867257219 1101 80-81 -0.3898078484261447 1101 82-83 -0.1818265795779972 1101 84-85 -0.05901903271541897 1101 86-87 0.1036537305883769 1101 88-89 0.1412114753168936 1101 90-91 0.17615820284435912 1101 92-93 0.09437234536605033 1101 94-95 -0.006755546603567808 1101 96-97 0.18687317603225395 1101 98-99 0.029212627305341243 1101 100-101 0.3991023524497912 1102 1 0.32085712567494795 1102 2 0.42371011452975793 1102 3 0.4019948024206563 1102 4 0.18629039266342318 1102 5 0.3180934178784369 1102 6 0.2977952265718997 1102 7 0.35410370977353267 1102 8 0.32688808578893 1102 9 0.46936857540831056 1102 10-11 0.6316692664619907 1102 12-13 0.4813917785534727 1102 14-15 0.5936874130723666 1102 16-17 0.47054227485404 1102 18-19 0.6140651526945007 1102 20-21 0.44886476719347 1102 22-23 0.344186556519567 1102 24-25 0.4296910741789972 1102 26-27 0.5065760409737834 1102 28-29 0.5598549276590674 1102 30-31 0.6265806032846939 1102 32-33 0.7478352607648802 1102 34-35 0.6319997198855347 1102 36-37 0.7224015786074887 1102 38-39 0.960306704886996 1102 40-41 0.7610821750074095 1102 42-43 0.7482132895341778 1102 44-45 1.1169668768207401 1102 46-47 1.2710636402756847 1102 48-49 1.418554703038879 1102 50-51 1.3316554643085041 1102 52-53 1.3479353001919279 1102 54-55 1.3281576427729789 1102 56-57 1.4654014485054674 1102 58-59 1.2917630759627556 1102 60-61 1.2305538842573824 1102 62-63 0.8005858527169707 1102 64-65 1.0802644932258758 1102 66-67 1.0940602878016676 1102 68-69 1.3671030342417723 1102 70-71 1.2328390005295233 1102 72-73 0.9817198377027232 1102 74-75 1.0181555578944987 1102 76-77 1.3142975544145301 1102 78-79 1.3260602897166969 1102 80-81 1.1182725321213027 1102 82-83 1.299028209249915 1102 84-85 1.2226856389950704 1102 86-87 1.2774901317407945 1102 88-89 1.4179621041917478 1102 90-91 1.7869171333482754 1102 92-93 1.630750891955696 1102 94-95 1.328649987495158 1102 96-97 1.4287651394204381 1102 98-99 1.6640010704372585 1102 100-101 1.6411867058510055 1103 1 -0.16705461570321134 1103 2 0.0416303606906645 1103 3 0.04187458874993766 1103 4 0.02004577982215494 1103 5 -0.2812644232445791 1103 6 0.03264190158408553 1103 7 0.02117013308237148 1103 8 -0.15188818108924096 1103 9 -0.042022099909665656 1103 10-11 0.048611462607119904 1103 12-13 -0.0055070336437239575 1103 14-15 0.04203519179512227 1103 16-17 0.09279374885711889 1103 18-19 0.1890816366835324 1103 20-21 0.18901020853435568 1103 22-23 0.13572143090679134 1103 24-25 0.26746473228673295 1103 26-27 0.35042010721553396 1103 28-29 0.06936308365015975 1103 30-31 0.0816868175096559 1103 32-33 0.13358688288495557 1103 34-35 0.18413451591408858 1103 36-37 0.1555864764067607 1103 38-39 0.21057188210182431 1103 40-41 0.24521932179613515 1103 42-43 0.17283845795754615 1103 44-45 0.05761973834567158 1103 46-47 -0.17934105359992714 1103 48-49 -0.41047527393131134 1103 50-51 -0.25732701966141747 1103 52-53 -0.4243945324836389 1103 54-55 -0.4387468074262699 1103 56-57 -0.321224063931588 1103 58-59 -0.24760976797925593 1103 60-61 -0.38672611421366554 1103 62-63 -0.4296715841899541 1103 64-65 -0.446301007935169 1103 66-67 -0.47253502787867063 1103 68-69 -0.5900505392003694 1103 70-71 -0.38327756277377034 1103 72-73 -0.28812306271487387 1103 74-75 -0.4015315807716817 1103 76-77 -0.26847357137025085 1103 78-79 -0.17662970617425344 1103 80-81 -0.2441745518849281 1103 82-83 0.053861123714735726 1103 84-85 -0.2900548122362103 1103 86-87 -0.25914015866408846 1103 88-89 -0.23230259522629382 1103 90-91 0.05483212374456059 1103 92-93 -0.09840044539749826 1103 94-95 -0.3800229797184258 1103 96-97 -0.24076193732328122 1103 98-99 -0.4257626112736368 1103 100-101 0.10368443631628566 1104 1 0.1449137118070425 1104 2 0.44507483889209354 1104 3 0.24666343881829889 1104 4 0.37392129250633843 1104 5 0.3212724140636425 1104 6 0.24642171322011563 1104 7 0.23355430443282188 1104 8 0.25610399572983766 1104 9 0.20413276869127372 1104 10-11 0.35738500810310114 1104 12-13 0.2805470070671916 1104 14-15 0.48834576448234657 1104 16-17 0.3155217983786187 1104 18-19 0.5087164916128941 1104 20-21 0.5293518081090269 1104 22-23 0.5518311073461106 1104 24-25 0.6502736719798818 1104 26-27 0.6470268974327666 1104 28-29 0.7512188105224098 1104 30-31 0.517943083649719 1104 32-33 0.7026093650359471 1104 34-35 0.6093680720435088 1104 36-37 0.496417024788947 1104 38-39 0.6319935363628062 1104 40-41 0.441995107911886 1104 42-43 0.28936492765220834 1104 44-45 0.3709172470802855 1104 46-47 0.5809316184341071 1104 48-49 0.7658880862388173 1104 50-51 0.6168137596185517 1104 52-53 0.7461536598299006 1104 54-55 0.5535344473208141 1104 56-57 0.7322992157281405 1104 58-59 0.585521023453218 1104 60-61 0.5926953121876721 1104 62-63 0.7064080907302852 1104 64-65 0.6148033517792442 1104 66-67 0.5604096781823955 1104 68-69 0.5954414477356735 1104 70-71 0.6335935446240697 1104 72-73 0.7612845022251271 1104 74-75 0.5084923444903566 1104 76-77 0.4963278109782223 1104 78-79 0.48297647641726726 1104 80-81 0.2833442465352469 1104 82-83 0.2905360819404663 1104 84-85 0.6790749538278966 1104 86-87 0.8675118237147643 1104 88-89 0.8114844319649563 1104 90-91 0.4616707611439246 1104 92-93 0.3858629983211692 1104 94-95 0.4138447477614484 1104 96-97 0.3643857916756552 1104 98-99 0.9033046832729212 1104 100-101 1.1975531132113169 1105 1 0.1848715254534099 1105 2 0.3295392556198955 1105 3 0.3589708489430201 1105 4 0.49306105773010955 1105 5 0.2870831257290831 1105 6 0.25415282107044845 1105 7 0.22685951353039968 1105 8 0.2491615892734913 1105 9 0.17105316487616307 1105 10-11 0.37783163319480906 1105 12-13 0.3993153489600729 1105 14-15 0.36573938150362295 1105 16-17 0.38056673601617774 1105 18-19 0.5169474160442888 1105 20-21 0.4585200032667629 1105 22-23 0.5410827581164668 1105 24-25 0.504267619155236 1105 26-27 0.42148581159270293 1105 28-29 0.5074688105224041 1105 30-31 0.3750413961955772 1105 32-33 0.32559175681878116 1105 34-35 0.6777374777661791 1105 36-37 0.7414005170853599 1105 38-39 0.4592174908749058 1105 40-41 0.5873179252266354 1105 42-43 0.3711560134922749 1105 44-45 0.4871773351213733 1105 46-47 0.6292258223959522 1105 48-49 0.6653378294523193 1105 50-51 0.9017413091416628 1105 52-53 0.6858501015026874 1105 54-55 0.7807629873061401 1105 56-57 0.8122561122798757 1105 58-59 0.6734887417217408 1105 60-61 0.8924706239998486 1105 62-63 0.7727232044500241 1105 64-65 0.237877453026492 1105 66-67 0.3390780567590639 1105 68-69 0.43911808016414255 1105 70-71 0.4452865013372076 1105 72-73 0.5235368866711987 1105 74-75 0.6895359981954208 1105 76-77 0.6097999313010405 1105 78-79 0.4195226979873361 1105 80-81 0.795463652257915 1105 82-83 0.6760047173036376 1105 84-85 0.7084173969680272 1105 86-87 0.34623522797008377 1105 88-89 0.4685644026179254 1105 90-91 0.7161874522664462 1105 92-93 0.7054658963402467 1105 94-95 0.4777800008795694 1105 96-97 0.5061777946103554 1105 98-99 0.39721517483565094 1105 100-101 0.3815314697777161 1106 1 0.06989194238872187 1106 2 0.16044404822681813 1106 3 0.10670349454800387 1106 4 0.046940275439119716 1106 5 -0.1417369903250716 1106 6 0.03485344432426274 1106 7 0.05862396657144586 1106 8 0.13652458089180186 1106 9 0.24421026782049893 1106 10-11 0.07469387261023996 1106 12-13 0.19012772807032263 1106 14-15 0.13518919882578473 1106 16-17 0.16777928705851508 1106 18-19 0.06357324884034199 1106 20-21 0.09853719647544779 1106 22-23 0.16896799344851132 1106 24-25 0.188890443139762 1106 26-27 -0.10571169539203851 1106 28-29 -0.13344028038668654 1106 30-31 -0.1002648578340839 1106 32-33 0.23471490317596988 1106 34-35 -0.11886686351900977 1106 36-37 -0.06522961401285698 1106 38-39 0.1559507812252221 1106 40-41 0.04935317130443906 1106 42-43 0.28067675280024673 1106 44-45 0.4047251537330254 1106 46-47 0.2821594136274115 1106 48-49 0.35056241574073965 1106 50-51 0.25432029044404203 1106 52-53 0.1804557322785243 1106 54-55 0.2126993724340167 1106 56-57 -0.1604711604474076 1106 58-59 0.06268925751800225 1106 60-61 0.3690341314144234 1106 62-63 0.2616980593823328 1106 64-65 0.0010624949349349322 1106 66-67 0.12882660608207885 1106 68-69 0.006676644531218301 1106 70-71 0.17681454775886607 1106 72-73 0.05438795050098477 1106 74-75 0.26600279381115044 1106 76-77 0.21982572111406284 1106 78-79 0.4614203983956706 1106 80-81 0.2877481998616105 1106 82-83 0.3875993874089474 1106 84-85 0.3270612826331849 1106 86-87 0.3462997025026411 1106 88-89 0.26052657755882436 1106 90-91 0.34679136372141883 1106 92-93 0.41435263607804984 1106 94-95 0.2044294743375552 1106 96-97 0.13001188014657217 1106 98-99 0.3581113708382304 1106 100-101 0.2874738725286292 1107 1 -0.08334098569450887 1107 2 0.0015739097159617188 1107 3 -0.16949562236967353 1107 4 -0.0010485953042476126 1107 5 -0.04451325597558764 1107 6 -0.25276525766569335 1107 7 -0.10125022444047005 1107 8 -0.2158615559254713 1107 9 -0.1385998694806574 1107 10-11 -0.10134193456329399 1107 12-13 -0.20934605164296727 1107 14-15 -0.06342569260348085 1107 16-17 -0.265439124603013 1107 18-19 -0.17487107463392704 1107 20-21 -0.2791196111214873 1107 22-23 -0.12595125895577297 1107 24-25 -0.19704677333637477 1107 26-27 -0.1565857049486823 1107 28-29 -0.19283059067519304 1107 30-31 0.16130291570973299 1107 32-33 0.24491268942166045 1107 34-35 0.414073316726558 1107 36-37 0.39487442034267417 1107 38-39 0.6133390350206582 1107 40-41 0.18040621672873414 1107 42-43 0.3185888074787684 1107 44-45 0.3566999919354146 1107 46-47 0.3260534437489895 1107 48-49 0.48875270183521025 1107 50-51 0.6976155181704087 1107 52-53 0.7045106527406304 1107 54-55 0.6405285625386483 1107 56-57 0.4691423398247778 1107 58-59 0.6484813097771251 1107 60-61 0.48028435823351145 1107 62-63 0.49490437402577214 1107 64-65 0.45920076385165487 1107 66-67 0.42835482237676104 1107 68-69 0.6994123851711507 1107 70-71 0.4078252536198761 1107 72-73 0.6302362538941999 1107 74-75 0.761103501486712 1107 76-77 1.0531409089202697 1107 78-79 0.8352847908229428 1107 80-81 0.9647336229548209 1107 82-83 1.0584334768059094 1107 84-85 1.1811591475094936 1107 86-87 1.322601644074048 1107 88-89 0.990333630120368 1107 90-91 0.9132826236258573 1107 92-93 0.7717227443463592 1107 94-95 0.7240024495991513 1107 96-97 1.0402882970098126 1107 98-99 0.6253949854272349 1107 100-101 0.6825443376589746 1108 1 0.10620538739781793 1108 2 0.2312264059656961 1108 3 0.07099234579338187 1108 4 -0.1185155247992924 1108 5 -0.0784205965738991 1108 6 0.03525497516057641 1108 7 0.0486735305340531 1108 8 0.27335876120424274 1108 9 0.2495430992402845 1108 10-11 0.06825055747127351 1108 12-13 0.16791805368674062 1108 14-15 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-1.675213879839589 2316 72-73 -1.9293254045478747 2316 74-75 -1.858343272717324 2316 76-77 -1.961656857111187 2316 78-79 -1.9487529967940098 2316 80-81 -2.036846279130259 2316 82-83 -1.7546488276405725 2316 84-85 -1.8050069567640428 2316 86-87 -1.4305337788910961 2316 88-89 -1.6800633609138487 2316 90-91 -1.9828388142800932 2316 92-93 -2.040303041216063 2316 94-95 -1.750260750385241 2316 96-97 -1.9905718068866278 2316 98-99 -2.5161606654859234 2316 100-101 -2.0675190534740686 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2833.0 3 484.0 4 451.0 5 435.0 6 490.0 7 547.0 8 699.0 9 791.0 10 987.0 11 1364.0 12 2119.0 13 3287.0 14 2972.0 15 3172.0 16 4270.0 17 5721.0 18 4916.0 19 3166.0 20 3104.0 21 3172.0 22 3467.0 23 3917.0 24 4451.0 25 5167.0 26 5810.0 27 6829.0 28 8002.0 29 9617.0 30 11531.0 31 13850.0 32 17473.0 33 21694.0 34 29445.0 35 44797.0 36 59661.0 37 96887.0 38 104769.0 39 17510.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 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19.50548426157249 18-19 16.983866852466097 32.76942552648624 32.43380895370061 17.81289866734706 20-21 19.26296513647667 29.481492676167463 30.768732544195203 20.48680964316066 22-23 18.150213332731337 29.18943377262765 32.256674561335494 20.403678333305525 24-25 20.641296335014808 30.04233623787606 31.049551950480442 18.26681547662869 26-27 19.502609450165245 27.939030052110482 34.825779816783914 17.732580680940362 28-29 17.04938403688221 31.7087271338845 32.95773210143982 18.284156727793476 30-31 18.472091496275826 29.581512303179068 31.94086072689406 20.005535473651047 32-33 18.64988625724509 29.973227762260958 32.94653599825706 18.430349982236898 34-35 20.36996405832921 28.86815713584338 31.943024629834245 18.81885417599317 36-37 19.530057919700976 28.34033837091477 33.942758460258574 18.186845249125685 38-39 18.85240732346623 29.948956465185944 33.04132752606373 18.15730868528409 40-41 18.135996823673484 30.438252271654736 33.093302620961985 18.332448283709798 42-43 17.624530512846036 30.750952771883693 30.830485940763065 20.794030774507206 44-45 18.876110800091467 28.888293132949073 31.21052569328907 21.025070373670392 46-47 20.560534890276138 30.45302856782419 30.97045448721613 18.015982054683544 48-49 19.361297121243336 29.78556451843447 31.492038393937094 19.361099966385098 50-51 16.840514624125667 30.50652151627225 33.62740613984591 19.025557719756172 52-53 18.275693818106546 31.863242302820616 30.512028151937663 19.349035727135174 54-55 17.54362102561525 29.425551774064623 31.66339934022675 21.367427860093375 56-57 19.84529428574244 29.40009656789807 30.293645241074863 20.46096390528463 58-59 21.264639032757344 28.543392226078783 30.672141823372996 19.519826917790876 60-61 19.25846992329033 29.629195567798288 32.60776433025044 18.504570178660945 62-63 18.351594091773247 29.687094997008828 32.43719774149389 19.524113169724032 64-65 18.17601996808025 31.411083585776684 31.15842664618861 19.254469799954457 66-67 19.010639514478495 29.485612205865536 29.631816801599477 21.871931478056496 68-69 17.837774946854452 29.48295990124197 31.50830805879731 21.170957093106264 70-71 20.496443061875443 29.67832264362093 29.701986265239615 20.123248029264012 72-73 19.391824280693452 27.81400805445879 32.63682474897594 20.15734291587182 74-75 18.11128341987659 31.9716778283969 30.047154325667506 19.869884426059002 76-77 19.028911487521363 29.003739353093888 30.619033445231388 21.34831571415336 78-79 18.988551686896816 29.092392557512547 31.57598081239863 20.343074943192008 80-81 20.32906659676432 29.142379051371325 30.223533110158275 20.30502124170608 82-83 19.049425910412456 28.80017740107426 32.08456117873158 20.0658355097817 84-85 19.113216305797266 29.26989364627407 30.829669446982304 20.787220600946352 86-87 18.886573918939696 29.95391523399156 30.751232935035855 20.408277912032894 88-89 19.39095740486666 29.136703411080095 29.63070287489552 21.841636309157717 90-91 19.704953101048385 27.70680638594658 30.640714106159706 21.947526406845324 92-93 20.924963991825333 29.381487509772242 30.49579387057563 19.197754627826804 94-95 19.98507303760727 28.412989809439676 30.679935954063602 20.92200119888945 96-97 18.51365126811773 28.454716278005947 31.545599198689196 21.486033255187127 98-99 20.04290415377669 29.286422688726027 29.074760882295532 21.595912275201755 100-101 20.053736044841802 27.27399961955038 30.716970122877342 21.955294212730475 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5128.0 1 3045.0 2 939.5 3 852.5 4 640.5 5 662.5 6 1108.5 7 1876.0 8 2590.0 9 2592.5 10 1918.5 11 1375.5 12 1318.5 13 1324.5 14 1244.5 15 1178.5 16 1195.5 17 1195.0 18 1143.5 19 1134.5 20 1278.0 21 1481.5 22 1664.0 23 1894.5 24 2160.0 25 2692.0 26 3812.5 27 5083.5 28 5596.5 29 6069.0 30 7568.0 31 9015.0 32 10557.5 33 13171.0 34 16306.5 35 18391.0 36 21006.0 37 23603.0 38 23057.0 39 21566.0 40 19714.5 41 17563.5 42 15881.5 43 14906.0 44 14522.0 45 14286.5 46 15887.5 47 25000.5 48 45671.0 49 44283.0 50 21415.0 51 9917.0 52 5694.5 53 4018.5 54 3086.5 55 2491.0 56 1947.0 57 1521.5 58 1257.5 59 973.5 60 760.0 61 614.0 62 483.5 63 376.5 64 296.0 65 217.0 66 173.5 67 165.0 68 146.5 69 131.5 70 114.0 71 102.5 72 89.5 73 66.5 74 49.5 75 39.5 76 33.0 77 24.5 78 15.0 79 14.5 80 13.0 81 7.5 82 5.0 83 3.0 84 2.5 85 1.0 86 0.5 87 0.5 88 0.0 89 0.5 90 1.0 91 0.5 92 0.5 93 0.5 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3361713728697542 2 0.0862983687646977 3 0.09669339954771809 4 0.10277351189250361 5 0.11611053251977509 6 0.05844753157245435 7 0.06080112344785519 8 0.0613895214167054 9 0.06452764391723986 10-11 0.06266438368254754 12-13 0.0613895214167054 14-15 0.059232062197587955 16-17 0.06080112344785519 18-19 0.06845029704290795 20-21 0.07021549094945857 22-23 0.08698483306168961 24-25 0.09502627196930916 26-27 0.09031908821850747 28-29 0.08894615962452365 30-31 0.0818853839983211 32-33 0.07374587876255985 34-35 0.32842413294655975 36-37 0.510925569618267 38-39 0.480328875238056 40-41 0.4617943392192744 42-43 0.4949407581311696 44-45 0.5034725286794977 46-47 0.49817694695984577 48-49 0.5184766768851781 50-51 0.5128868961811011 52-53 0.5122004318841091 54-55 0.5027860643825057 56-57 0.47934821195663896 58-59 0.4617943392192744 60-61 0.42472526718171105 62-63 0.4967059520377203 64-65 0.5196534728228784 66-67 0.5281852433712065 68-69 0.541522263998478 70-71 0.5396590037637857 72-73 0.5269103811053644 74-75 0.5284794423556316 76-77 0.5005305388352467 78-79 0.4801327425817726 80-81 0.4872915845361169 82-83 0.49700015102214534 84-85 0.49866727860055426 86-87 0.49680401836586197 88-89 0.5042570593046313 90-91 0.5200457381354453 92-93 0.5261258504802308 94-95 0.5340692230597087 96-97 0.5275968454023563 98-99 0.5260277841520891 100-101 0.5029821970387892 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 509859.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.43807419762758 #Duplication Level Percentage of deduplicated Percentage of total 1 78.69883250387957 26.315374005313906 2 10.434297306063112 6.978056150404878 3 3.4004254104781557 3.411110315372004 4 1.6365664880207413 2.1889452662315336 5 0.9981223750160778 1.6687645017049935 6 0.6943655314496782 1.393094769653367 7 0.4917928074129687 1.1511223068894094 8 0.3877613843488076 1.0372795152664172 9 0.2943122228705118 0.8857110551051591 >10 2.4239061273910387 16.74557557168466 >50 0.322011082440303 7.477186140758008 >100 0.1906258684755892 11.024946609233812 >500 0.010557740407878788 2.419043485995941 >1k 0.014663528344276092 12.559648674899309 >5k 0.0011730822675420874 2.5196571805245545 >10k+ 5.865411337710437E-4 2.2244844509620534 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 11342 2.2245365875663663 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 7674 1.5051220043188411 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5173 1.014594230954048 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4380 0.8590610345213088 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4115 0.8070858806062068 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3942 0.773154931069178 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3567 0.6996051849629015 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3535 0.6933289399618325 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3439 0.6745002049586257 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3201 0.6278206327631758 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3097 0.6074228365097017 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 3000 0.5883979688502116 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2956 0.5797681319737418 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2852 0.5593703357202677 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2707 0.5309311005591743 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2349 0.46071560960971564 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2235 0.43835648679340755 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2232 0.43776808882455737 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 2191 0.42972664991693776 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 2072 0.40638686381921274 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1842 0.36127635287402987 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1814 0.3557846384980946 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1701 0.33362164833806995 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1499 0.2940028517688224 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 1480 0.2902763312994377 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 1428 0.2800774331727007 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 1218 0.23888957535318586 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 1186 0.23261333035211698 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 952 0.18671828878180047 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 919 0.18024591112444813 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 823 0.16141717612124135 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 807 0.15827905362070688 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 778 0.15259120658848818 No Hit GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 767 0.15043374736937073 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 717 0.14062711455520055 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 670 0.13140887970988058 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 660 0.12944755314704653 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 652 0.1278784918967793 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 640 0.12552490002137848 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 605 0.11866025705145931 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 603 0.1182679917388925 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 577 0.11316854267552402 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 567 0.11120721611268998 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 540 0.10591163439303808 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 532 0.10434257314277084 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 525 0.10296964454878701 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.9613265628340385E-4 0.0 12-13 0.0 0.0 0.0 1.9613265628340385E-4 0.0 14-15 0.0 0.0 0.0 1.9613265628340385E-4 0.0 16-17 0.0 0.0 0.0 2.941989844251058E-4 0.0 18-19 0.0 0.0 0.0 4.903316407085096E-4 0.0 20-21 0.0 0.0 0.0 8.825969532753172E-4 0.0 22-23 0.0 0.0 0.0 0.002451658203542548 0.0 24-25 0.0 0.0 0.0 0.0057859133603604136 0.0 26-27 0.0 0.0 0.0 0.011866025705145932 0.0 28-29 0.0 0.0 0.0 0.03108702602091951 0.0 30-31 0.0 0.0 0.0 0.0820815166546045 0.0 32-33 0.0 0.0 0.0 0.1623978394026584 0.0 34-35 0.0 0.0 0.0 0.274389586140482 0.0 36-37 0.0 0.0 0.0 0.43100151218277993 0.0 38-39 0.0 0.0 0.0 0.6477280973759412 0.0 40-41 0.0 0.0 0.0 0.8970127035121475 0.0 42-43 0.0 0.0 0.0 1.1440417841010948 0.0 44-45 0.0 0.0 0.0 1.4209810947732608 0.0 46-47 0.0 0.0 0.0 1.7383237326398082 0.0 48-49 0.0 0.0 0.0 2.034484043627748 0.0 50-51 0.0 0.0 0.0 2.3422161813364086 0.0 52-53 0.0 0.0 0.0 2.6329828442765546 0.0 54-55 0.0 0.0 0.0 2.9453240994078755 0.0 56-57 0.0 0.0 0.0 3.3610272644005503 0.0 58-59 0.0 0.0 0.0 3.7742787711896817 0.0 60-61 0.0 0.0 0.0 4.2529405188493286 0.0 62-63 0.0 0.0 0.0 4.701790102753899 0.0 64-65 0.0 0.0 0.0 5.153189411190152 0.0 66-67 0.0 0.0 0.0 5.6698028278406385 0.0 68-69 0.0 0.0 0.0 6.277323730678482 0.0 70-71 0.0 0.0 0.0 6.943390231416922 0.0 72-73 0.0 0.0 0.0 7.5630713589443355 0.0 74-75 0.0 0.0 0.0 8.275621299221942 0.0 76-77 0.0 0.0 0.0 8.94678724902375 0.0 78-79 0.0 0.0 0.0 9.84115216167607 0.0 80-81 0.0 0.0 0.0 10.739439727454062 0.0 82-83 0.0 0.0 0.0 11.56162782259409 0.0 84-85 0.0 0.0 0.0 12.479724786656703 0.0 86-87 0.0 0.0 0.0 13.423318996036159 0.0 88-89 0.0 0.0 0.0 14.424478140034793 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAAGGTG 85 3.7040445E-7 39.024406 5 GACGTGA 110 7.203744E-8 34.432667 7 GTAAGCG 70 4.4929038E-10 33.972855 94-95 GCATCAC 35 2.0929902E-4 33.817795 12-13 CGCAGAA 130 8.536517E-9 32.777252 9 GATAGCG 30 0.0039246418 31.709562 92-93 GTAAGGT 90 2.4499785E-5 31.591183 4 ACCCGAA 60 0.0040126266 31.563278 7 CCCGAAT 60 0.0040126266 31.563278 8 CTATACA 125 2.1953565E-7 30.327538 4 CATTCCG 125 2.2124368E-7 30.300745 9 TACAATC 80 4.576985E-4 29.59057 7 AACCCGA 85 6.5258396E-4 27.84995 6 AGGTGGC 125 7.57019E-6 26.513153 7 CGCAGCC 90 9.1130176E-4 26.30273 8 ACTATAC 185 9.173164E-9 25.614473 3 TAGGACG 185 9.173164E-9 25.614473 4 CCGACCT 65 9.907302E-6 25.614096 64-65 GGACGTG 190 1.2298187E-8 24.918375 6 GAGTACT 2570 0.0 24.685675 12-13 >>END_MODULE