##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938924_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 514079 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.884578051233373 33.0 31.0 34.0 30.0 34.0 2 31.933311806162088 33.0 31.0 34.0 30.0 34.0 3 31.96290064367539 34.0 31.0 34.0 30.0 34.0 4 35.478115231316586 37.0 35.0 37.0 33.0 37.0 5 35.522133757652036 37.0 35.0 37.0 33.0 37.0 6 35.64693364249464 37.0 35.0 37.0 33.0 37.0 7 35.59432499674175 37.0 35.0 37.0 33.0 37.0 8 35.59914332232984 37.0 35.0 37.0 33.0 37.0 9 37.19313957582395 39.0 37.0 39.0 34.0 39.0 10-11 37.10353467074127 39.0 37.0 39.0 33.5 39.0 12-13 37.03869152406537 39.0 37.0 39.0 33.0 39.0 14-15 38.1736824495846 40.0 38.0 41.0 33.0 41.0 16-17 38.12798616555043 40.0 38.0 41.0 33.0 41.0 18-19 38.13317505675198 40.0 38.0 41.0 33.5 41.0 20-21 38.0182180170752 40.0 38.0 41.0 33.0 41.0 22-23 38.09122916905767 40.0 38.0 41.0 33.5 41.0 24-25 37.99415264969002 40.0 38.0 41.0 33.0 41.0 26-27 37.86853090672834 40.0 37.5 41.0 33.0 41.0 28-29 37.762444099058705 40.0 37.0 41.0 33.0 41.0 30-31 37.613650820204676 40.0 37.0 41.0 33.0 41.0 32-33 37.37729609651435 40.0 36.5 41.0 33.0 41.0 34-35 37.035480928028576 40.0 36.0 41.0 31.5 41.0 36-37 36.72449759667289 39.5 35.5 41.0 30.5 41.0 38-39 36.50722457054266 39.0 35.0 41.0 30.0 41.0 40-41 36.448524448576975 39.0 35.0 41.0 30.0 41.0 42-43 36.233995164167375 39.0 35.0 41.0 30.0 41.0 44-45 36.08132213142338 39.5 35.0 41.0 30.0 41.0 46-47 35.79257079164876 39.5 35.0 41.0 27.5 41.0 48-49 35.551335495128185 39.0 35.0 41.0 27.0 41.0 50-51 34.37812184508607 38.0 34.0 40.0 24.0 40.5 52-53 34.58375269170692 38.5 34.5 39.5 24.0 40.5 54-55 35.15466202665349 39.0 35.0 40.5 25.0 41.0 56-57 35.06437629235973 39.0 35.0 41.0 23.5 41.0 58-59 34.94051011614946 39.0 35.0 41.0 23.5 41.0 60-61 34.62746970796317 39.0 35.0 40.5 22.5 41.0 62-63 34.32520779880135 38.0 34.5 40.0 22.0 41.0 64-65 33.94375767148629 38.0 34.0 40.0 21.5 41.0 66-67 33.5023420524861 37.0 34.0 40.0 19.5 41.0 68-69 33.087453484775686 37.0 33.0 39.0 18.0 41.0 70-71 32.557541739693704 36.0 33.0 39.0 13.0 40.5 72-73 32.06262461606096 36.0 32.0 38.5 8.0 40.0 74-75 31.582856914987772 35.0 32.0 37.0 4.5 39.5 76-77 31.141003620066176 35.0 31.5 37.0 2.0 39.0 78-79 30.630706564555254 35.0 31.0 36.0 2.0 39.0 80-81 30.114336512481543 35.0 31.0 36.0 2.0 38.5 82-83 29.667933333203653 34.5 30.0 35.5 2.0 37.0 84-85 29.223167061871813 34.0 30.0 35.0 2.0 37.0 86-87 28.87200021786535 34.0 29.5 35.0 2.0 36.0 88-89 28.367398785011645 34.0 29.0 35.0 2.0 36.0 90-91 27.86673157238479 34.0 28.5 35.0 2.0 36.0 92-93 27.443311242046455 34.0 27.0 35.0 2.0 35.0 94-95 26.927376920667832 34.0 26.0 35.0 2.0 35.0 96-97 26.30179991791145 34.0 25.0 35.0 2.0 35.0 98-99 25.694625728730408 33.0 22.0 35.0 2.0 35.0 100-101 23.17194341725688 30.0 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3410789381198853 1101 2 0.1273932990562976 1101 3 0.320678141554545 1101 4 -0.03991628807820291 1101 5 0.01242892432144771 1101 6 0.33364560918990804 1101 7 0.15354322868245163 1101 8 0.19622919647503068 1101 9 0.1553702255610645 1101 10-11 0.18078095397614646 1101 12-13 0.3870140520976406 1101 14-15 0.10921238677889278 1101 16-17 0.07280907395745828 1101 18-19 0.23629558587178678 1101 20-21 0.42222742335331986 1101 22-23 0.4542982068447756 1101 24-25 0.47576841743128284 1101 26-27 0.5054459075587587 1101 28-29 0.4962480058375647 1101 30-31 0.537674888943684 1101 32-33 0.700550844624722 1101 34-35 0.36508616662209903 1101 36-37 0.41321463044739204 1101 38-39 0.4271042290520697 1101 40-41 0.40372591634598365 1101 42-43 0.2751402071426341 1101 44-45 0.5355074494542649 1101 46-47 0.5761576335291565 1101 48-49 0.7079522217544607 1101 50-51 0.8362384961769536 1101 52-53 0.9063453069184391 1101 54-55 0.8702935153315181 1101 56-57 0.7228292891491961 1101 58-59 0.4463782164117873 1101 60-61 0.6806002294270286 1101 62-63 0.6026212603067833 1101 64-65 0.6661333690684472 1101 66-67 0.6919005206571924 1101 68-69 0.8985643887006702 1101 70-71 0.897241054769438 1101 72-73 0.6290851973092515 1101 74-75 0.7476176774228769 1101 76-77 0.7144708034955904 1101 78-79 0.5611372920114448 1101 80-81 0.5182440024605697 1101 82-83 0.7737799287957188 1101 84-85 0.6804577515944317 1101 86-87 0.7340265773938128 1101 88-89 0.6374303228168081 1101 90-91 0.4687858219433316 1101 92-93 0.8099604466361754 1101 94-95 0.8743982041404799 1101 96-97 0.5145920918206563 1101 98-99 0.4705393442164514 1101 100-101 0.4990567139637001 1102 1 0.1658002350330534 1102 2 0.17774639699100803 1102 3 -0.07158479426601971 1102 4 0.19701908833751958 1102 5 0.3544098259425894 1102 6 0.2409962643087198 1102 7 0.1455396754956837 1102 8 0.2660448749116284 1102 9 0.3671579226518915 1102 10-11 0.14849358999656914 1102 12-13 0.1660058353532463 1102 14-15 0.1442845608850405 1102 16-17 0.3480633488852902 1102 18-19 0.4575014486299409 1102 20-21 0.4369353958161213 1102 22-23 0.31933929056673804 1102 24-25 0.42727852180614434 1102 26-27 0.18873038457408597 1102 28-29 0.23346319571098917 1102 30-31 0.2572129746643128 1102 32-33 0.22022439559074058 1102 34-35 0.22828181396831582 1102 36-37 0.24959260068944644 1102 38-39 0.1681679643396521 1102 40-41 0.09969304936841183 1102 42-43 0.1602723412754372 1102 44-45 0.2288341205624178 1102 46-47 0.03416784894110947 1102 48-49 0.06934018533429764 1102 50-51 -0.17590896107376608 1102 52-53 -0.1548094787799812 1102 54-55 0.08377563836061341 1102 56-57 -0.00948472373110576 1102 58-59 0.04124608227898818 1102 60-61 -0.17804067663558953 1102 62-63 -0.36151154004853225 1102 64-65 -0.21939183635016235 1102 66-67 -0.053851200417646794 1102 68-69 -0.3056683450323945 1102 70-71 -0.24481313132871918 1102 72-73 0.06435279668744442 1102 74-75 0.027846413820832083 1102 76-77 -0.0010788300820259167 1102 78-79 0.06702003684821989 1102 80-81 -0.22102093202754958 1102 82-83 -0.2829911127321516 1102 84-85 -0.3667552175372606 1102 86-87 -0.46623991161351697 1102 88-89 -0.3973664793150995 1102 90-91 -0.10350376277796514 1102 92-93 0.11687583637634802 1102 94-95 -0.1892815649023838 1102 96-97 -0.13763531213226443 1102 98-99 -0.08659367821303832 1102 100-101 -0.2518560108863994 1103 1 -0.021126831059849138 1103 2 0.263144168148461 1103 3 0.21590941295013266 1103 4 0.2653937986983266 1103 5 0.1850133788500159 1103 6 0.061055847228281834 1103 7 0.16107978362764896 1103 8 0.04211460899526287 1103 9 0.29750328290788275 1103 10-11 0.3379937555046837 1103 12-13 0.39658070018829505 1103 14-15 0.2496271626725317 1103 16-17 0.48840098542947885 1103 18-19 0.3300447565853659 1103 20-21 0.4284304857421901 1103 22-23 0.5448297462657408 1103 24-25 0.4608877019893072 1103 26-27 0.28465005797141174 1103 28-29 0.23635627747199095 1103 30-31 0.20925093119085147 1103 32-33 0.2855294822066483 1103 34-35 0.12934989297085764 1103 36-37 0.009312340290016152 1103 38-39 0.22902419296152488 1103 40-41 0.4115941858574317 1103 42-43 0.3837634590068717 1103 44-45 0.24408293442096607 1103 46-47 -0.0251745321688972 1103 48-49 0.15235022615963345 1103 50-51 0.2181879163398932 1103 52-53 0.3473399198738818 1103 54-55 0.5363564614876069 1103 56-57 0.5225201311734509 1103 58-59 0.4132266626607546 1103 60-61 0.3639056987368008 1103 62-63 0.2737129992872198 1103 64-65 0.17059161283884805 1103 66-67 0.17157305203739526 1103 68-69 0.060971840336684124 1103 70-71 0.02108337093322632 1103 72-73 0.29104815142649443 1103 74-75 -0.014801715937526438 1103 76-77 -0.03638502008533706 1103 78-79 0.07661509367266817 1103 80-81 0.17452800320361206 1103 82-83 0.22158132465061797 1103 84-85 -0.0025160031490898405 1103 86-87 0.10325142679539923 1103 88-89 0.16764951980218612 1103 90-91 0.36288926381367403 1103 92-93 0.4993827325475948 1103 94-95 0.3309104245050989 1103 96-97 0.24745571732818306 1103 98-99 -0.11733184438318389 1103 100-101 0.33857320681193315 1104 1 0.20230437882812424 1104 2 0.24143383644854666 1104 3 0.1982376145166711 1104 4 0.15673924763145664 1104 5 0.1731935951816368 1104 6 0.2413622098474093 1104 7 0.04101701916160039 1104 8 0.09282778310235074 1104 9 0.4334629490214965 1104 10-11 0.29929328862966287 1104 12-13 0.2833667222330263 1104 14-15 0.05888363525809126 1104 16-17 -0.131308085836487 1104 18-19 0.2661356107222872 1104 20-21 0.09256342293914344 1104 22-23 0.18521379277317607 1104 24-25 0.1577919218707251 1104 26-27 0.25064406447936705 1104 28-29 0.2429928480627197 1104 30-31 0.24055599479131473 1104 32-33 0.319516183342337 1104 34-35 0.35643378899791855 1104 36-37 -0.04037833892953557 1104 38-39 -0.01649676496827368 1104 40-41 0.2948625682576562 1104 42-43 0.36423316357659274 1104 44-45 0.4290709088521609 1104 46-47 0.6811122036930186 1104 48-49 0.6320610980455186 1104 50-51 0.23286296875079415 1104 52-53 0.1696830408626795 1104 54-55 0.13626514433413206 1104 56-57 0.35833574769284837 1104 58-59 0.40432002480400797 1104 60-61 0.5071514447722194 1104 62-63 0.3614224821234586 1104 64-65 0.22673236987090917 1104 66-67 0.182736895589251 1104 68-69 0.5086702622219335 1104 70-71 0.6423615538504848 1104 72-73 0.41595972812648085 1104 74-75 0.2550404725873783 1104 76-77 0.3253273694659242 1104 78-79 0.46280305266997956 1104 80-81 0.34286024330342 1104 82-83 0.31162518260742544 1104 84-85 0.3463219539189879 1104 86-87 0.5931293704874427 1104 88-89 0.8328756905266808 1104 90-91 0.9850163104111438 1104 92-93 0.8657317184127287 1104 94-95 0.8453218147122996 1104 96-97 0.30677971316103125 1104 98-99 0.1554620746660902 1104 100-101 0.7153079111887273 1105 1 0.057642867666743314 1105 2 -0.025025462517483277 1105 3 0.15410026005314137 1105 4 0.1095979302318355 1105 5 0.24494036069630454 1105 6 0.27911460625576723 1105 7 0.21959093591124912 1105 8 0.21639188472072135 1105 9 0.3817808527225637 1105 10-11 0.3152249474483213 1105 12-13 0.30290067766945583 1105 14-15 0.260479055136706 1105 16-17 0.3145816486426938 1105 18-19 0.5184992336737153 1105 20-21 0.23616965380578137 1105 22-23 0.274664678184088 1105 24-25 0.28074334619175545 1105 26-27 0.14272679845448266 1105 28-29 0.11534696324606131 1105 30-31 0.026682439910587163 1105 32-33 0.027451481341188355 1105 34-35 0.07840943763670793 1105 36-37 0.10657815073691523 1105 38-39 0.1488113563869078 1105 40-41 -0.008879885975964896 1105 42-43 -0.10473135031827496 1105 44-45 0.09112712762918562 1105 46-47 0.26828450330246056 1105 48-49 -0.0435867211101737 1105 50-51 -0.11817243539082511 1105 52-53 -0.09408170744784883 1105 54-55 -0.04834333643108124 1105 56-57 -0.0010763279922656466 1105 58-59 -0.034471669013072415 1105 60-61 -0.0365556012216075 1105 62-63 -0.09392584233732038 1105 64-65 0.02262271907648028 1105 66-67 -0.23355376343842238 1105 68-69 -0.3073749160692003 1105 70-71 -0.35204443604340696 1105 72-73 -0.4244163204942808 1105 74-75 -0.18782070990029354 1105 76-77 -0.3850319995789171 1105 78-79 -0.340951696091345 1105 80-81 -0.3259866529430617 1105 82-83 -0.42993974684247505 1105 84-85 -0.13848815710059625 1105 86-87 -0.0849404706262753 1105 88-89 -0.22371602050273864 1105 90-91 -0.2525344557428433 1105 92-93 0.09577257722646948 1105 94-95 0.27179848994415323 1105 96-97 0.13843894163843729 1105 98-99 0.18906711706172175 1105 100-101 0.3896607067594182 1106 1 -0.1492367550324687 1106 2 0.03708268952958349 1106 3 0.08614507256974235 1106 4 0.11081321220139273 1106 5 0.13006402167287945 1106 6 -0.10049602062047569 1106 7 0.14703618786093386 1106 8 0.14984111249353305 1106 9 0.13002835173665517 1106 10-11 0.10934330464464637 1106 12-13 0.058713508149693894 1106 14-15 0.007450910494007701 1106 16-17 0.1448948855582941 1106 18-19 -0.014212776692403395 1106 20-21 0.009919965674505704 1106 22-23 0.07572060529935953 1106 24-25 0.16194349749838466 1106 26-27 0.29047208823082116 1106 28-29 0.2688447218154053 1106 30-31 0.2218800640745826 1106 32-33 0.23926265388708856 1106 34-35 0.23944435888482474 1106 36-37 0.42969828659094844 1106 38-39 0.23559134206106336 1106 40-41 0.38168839918874653 1106 42-43 0.5221408680139703 1106 44-45 0.7030256234159324 1106 46-47 0.38502601846129636 1106 48-49 0.4224558057105341 1106 50-51 0.1977224065018035 1106 52-53 0.1901514174364607 1106 54-55 0.030326050535627758 1106 56-57 0.23913501839528806 1106 58-59 0.2586145033316143 1106 60-61 0.41050719907778443 1106 62-63 0.489942697956117 1106 64-65 0.12198208036934943 1106 66-67 -0.025106748927470335 1106 68-69 -0.04069899394382759 1106 70-71 0.0068440236068099125 1106 72-73 0.16332341600683264 1106 74-75 0.2217293138419727 1106 76-77 0.3785956573413536 1106 78-79 0.3966057481143359 1106 80-81 0.5842675904835488 1106 82-83 0.7372540785593316 1106 84-85 0.4239761289011241 1106 86-87 0.34037602582042936 1106 88-89 0.2796320410822766 1106 90-91 0.6352491435843284 1106 92-93 0.7222806133790982 1106 94-95 0.947793049344078 1106 96-97 0.9967358567538085 1106 98-99 0.891590637999176 1106 100-101 1.249247349925266 1107 1 -0.15171358843612381 1107 2 -0.15138323643106943 1107 3 -0.20849652885541303 1107 4 -0.12790821045420842 1107 5 -0.19061167723169348 1107 6 -0.2020305634611006 1107 7 -0.32518733658704946 1107 8 -0.33393055003205063 1107 9 -0.2026720998752367 1107 10-11 -0.15960798690287703 1107 12-13 -0.17457372639158564 1107 14-15 -0.27917028244897324 1107 16-17 -0.07051539369099658 1107 18-19 0.0016873998894126885 1107 20-21 -0.3161938280216816 1107 22-23 -0.3457395544056112 1107 24-25 -0.2320292807742419 1107 26-27 -0.2564119459604157 1107 28-29 -0.2506613179076922 1107 30-31 -0.24885501632483908 1107 32-33 -0.4292206177207447 1107 34-35 -0.20063893147506207 1107 36-37 -0.22553744231689876 1107 38-39 0.0014446385153732422 1107 40-41 0.09511799305057167 1107 42-43 0.010569289872613297 1107 44-45 -0.05844828779310518 1107 46-47 -0.14525463723541776 1107 48-49 -0.2679790856056954 1107 50-51 -0.1711577835189857 1107 52-53 -0.13925961189487168 1107 54-55 -0.3978770341767657 1107 56-57 -0.27321398852733836 1107 58-59 -0.27543218927539215 1107 60-61 -0.3703282047945109 1107 62-63 -0.3633771964073347 1107 64-65 -0.13927305691129277 1107 66-67 -0.19678177588354373 1107 68-69 -0.40478569092631034 1107 70-71 -0.5016490928277975 1107 72-73 -0.7297000396836353 1107 74-75 -0.45890763082385533 1107 76-77 -0.09086584626060557 1107 78-79 -0.29922817122059087 1107 80-81 -0.5907710856216504 1107 82-83 -0.5300134350749737 1107 84-85 -0.5443407182540341 1107 86-87 -0.4820498645113247 1107 88-89 -0.671206331864763 1107 90-91 -0.6810237300112227 1107 92-93 -0.9373305940967214 1107 94-95 -0.9999512351870443 1107 96-97 -0.9062652527425463 1107 98-99 -0.17251012302156 1107 100-101 0.23574587320124962 1108 1 0.3054691654235455 1108 2 0.2386632051404156 1108 3 0.12683201388338716 1108 4 0.3491455739062772 1108 5 0.33037332339589653 1108 6 0.2526261454632177 1108 7 0.17645223916291997 1108 8 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-1.6096475015994436 2316 72-73 -1.9382939159407684 2316 74-75 -1.9089082523974277 2316 76-77 -1.8337457209513737 2316 78-79 -1.5399263226607758 2316 80-81 -1.42812703594549 2316 82-83 -1.2731357234602463 2316 84-85 -1.7273319789295556 2316 86-87 -1.655335253802857 2316 88-89 -1.86948519463375 2316 90-91 -2.1347413615672686 2316 92-93 -2.404430704736818 2316 94-95 -2.2313195672565094 2316 96-97 -2.527450344921906 2316 98-99 -2.9655696324756917 2316 100-101 -3.793113787916834 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2964.0 3 492.0 4 449.0 5 418.0 6 484.0 7 563.0 8 697.0 9 814.0 10 973.0 11 1401.0 12 2050.0 13 3247.0 14 3181.0 15 3161.0 16 4226.0 17 5841.0 18 4783.0 19 3118.0 20 3047.0 21 3162.0 22 3479.0 23 3817.0 24 4228.0 25 4902.0 26 5619.0 27 6676.0 28 8009.0 29 9408.0 30 11192.0 31 13740.0 32 16899.0 33 21349.0 34 28585.0 35 44014.0 36 59557.0 37 97469.0 38 110055.0 39 20003.0 40 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.40945947791078 22.55637631151362 16.439783018739114 18.59438119183648 2 13.704494703581629 25.0949578408602 38.500026282539245 22.700521173018927 3 20.855674318088894 28.119463426980513 33.22780990206958 17.79705235286101 4 10.961502138986411 19.53399888231388 42.9088267995428 26.595672179156903 5 9.997935960245815 40.87285515114223 36.6668873501624 12.462321538449556 6 24.337657129396558 41.00773065923776 18.13580340647382 16.518808804891865 7 21.69697441688408 36.80717261788871 24.791285386083096 16.704567579144115 8 22.418599942371642 32.10697848281662 22.420157465598205 23.05426410921353 9 22.210453764889586 21.09911334192604 26.612787034621334 30.077645858563045 10-11 22.155390764474433 27.397025583717998 29.638545538083548 20.809038113724014 12-13 26.29870256294439 26.96416472622209 28.52628750769039 18.21084520314313 14-15 21.907692727063857 28.97803496570215 30.735958129944375 18.378314177289624 16-17 17.167746702216824 31.8400710224369 31.49216231975477 19.500019955591508 18-19 16.976347075705714 32.79448358041566 32.499406145710424 17.729763198168204 20-21 19.29785106716474 29.407171806561973 30.841513647811787 20.453463478461504 22-23 18.111111760226983 29.25412691643347 32.24544758811261 20.389313735226935 24-25 20.645569028578997 29.991489013621475 31.109408048238013 18.25353390956152 26-27 19.554357098617555 27.85253526229068 34.89311270299432 17.699994936097447 28-29 17.05976404419984 31.607385595712444 33.02446415286882 18.30838620721889 30-31 18.438937377561235 29.683733121463153 31.89863335942764 19.978696141547978 32-33 18.725081370430914 29.92465141910226 32.840064142195494 18.510203068271323 34-35 20.345158515761756 28.919253760923496 31.932874020168256 18.80271370314649 36-37 19.554118131596685 28.233837051298234 33.972739163313605 18.239305653791483 38-39 18.856320130205482 30.03900687021706 32.98349739432542 18.121175605252038 40-41 18.154237543337512 30.438038846835997 33.180156943876824 18.22756666594967 42-43 17.57404343046705 30.862839214949904 30.752913424638244 20.8102039299448 44-45 18.843639095508454 28.931325786773826 31.28010439450794 20.94493072320978 46-47 20.684987377899148 30.388404685777665 30.95471203906836 17.97189589725483 48-49 19.36216673123657 29.670556514320822 31.576934064342932 19.390342690099672 50-51 16.817870558772892 30.409297229613397 33.707446392236655 19.065385819377052 52-53 18.31841291487682 31.891183827838166 30.45007782226783 19.340325435017185 54-55 17.477741498142436 29.434728936345866 31.698532533311948 21.388997032199754 56-57 19.803410203229493 29.30912924136326 30.338331196700295 20.54912935870695 58-59 21.16338013294767 28.62941472124601 30.63888416264284 19.56832098316348 60-61 19.287877618075434 29.50989178312059 32.73704754486559 18.46518305393839 62-63 18.253066254572484 29.679485925549187 32.497603724496784 19.569844095381544 64-65 18.217663300308978 31.447038081391366 31.130329115113 19.204969503186657 66-67 18.98676332010024 29.498998466631377 29.67347174423372 21.840766469034662 68-69 17.907366109652553 29.384100976870393 31.448807509660575 21.25972540381648 70-71 20.51221082584284 29.596893035161777 29.769306490847995 20.121589648147385 72-73 19.492712785109347 27.828883514143925 32.61234130152319 20.066062399223533 74-75 18.125382188195584 31.86292591052516 30.12156642125089 19.890125480028374 76-77 19.01994427966599 29.096070620827824 30.50336121958664 21.38062387991955 78-79 19.1098462621758 29.047646989789932 31.46510581700113 20.377400931033137 80-81 20.28757180493701 29.192361930132353 30.2667222134208 20.253344051509835 82-83 19.191499308986305 28.86461511075292 31.900282762998444 20.043602817262336 84-85 19.019628557957365 29.36702644856723 30.85027086353525 20.763074129940154 86-87 18.976992753446 29.926243401664877 30.67124275124533 20.425521093643788 88-89 19.40356032578605 29.265083647504664 29.547202000262164 21.784154026447126 90-91 19.736311729784095 27.750568428632366 30.708060533246716 21.805059308336823 92-93 21.003044926362097 29.277475763873394 30.568144904053508 19.151334405710998 94-95 20.04166152991157 28.443338166775273 30.64969678468484 20.86530351862832 96-97 18.52077970489561 28.434299109951382 31.659979316667304 21.384941868485704 98-99 20.18956520547972 29.211377826135028 29.122436579215925 21.476620389169323 100-101 19.897269955423653 27.402080229601943 30.66465419337547 22.03599562159894 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5323.0 1 3158.5 2 977.5 3 869.0 4 646.0 5 642.0 6 1047.0 7 1855.5 8 2587.0 9 2583.5 10 1942.5 11 1434.5 12 1337.5 13 1313.5 14 1234.5 15 1183.5 16 1262.0 17 1262.5 18 1174.0 19 1181.5 20 1267.5 21 1467.5 22 1704.5 23 1932.5 24 2168.5 25 2642.5 26 3807.0 27 5093.5 28 5625.0 29 6143.0 30 7626.0 31 9129.0 32 10771.0 33 13348.5 34 16450.0 35 18708.0 36 21207.0 37 23767.5 38 23262.5 39 21550.0 40 19763.0 41 17733.5 42 15978.0 43 15057.0 44 14788.0 45 14381.5 46 15922.0 47 25061.0 48 46089.0 49 44667.5 50 21481.0 51 10157.5 52 5936.0 53 4073.5 54 3062.5 55 2438.0 56 1902.5 57 1547.0 58 1221.5 59 948.0 60 768.5 61 594.0 62 449.0 63 332.0 64 263.0 65 217.5 66 170.5 67 161.5 68 150.5 69 136.5 70 133.5 71 114.5 72 89.5 73 64.0 74 43.0 75 36.5 76 32.0 77 27.0 78 17.0 79 12.5 80 11.0 81 6.0 82 5.0 83 4.0 84 2.5 85 1.5 86 1.5 87 0.5 88 0.5 89 0.5 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2738878654837097 2 0.08364473164630339 3 0.08734066164927959 4 0.10076272323903525 5 0.1017353363977132 6 0.0797542790115916 7 0.08928588796663547 8 0.08617352585886605 9 0.09045302375704901 10-11 0.08403377690977457 12-13 0.08772970691275077 14-15 0.09531608955043874 16-17 0.0852981740160559 18-19 0.0943434763917608 20-21 0.09696953192019125 22-23 0.10893267377193 24-25 0.1215766448347433 26-27 0.12468900694251271 28-29 0.11535192061920443 30-31 0.10834910587672322 32-33 0.1035833013992013 34-35 0.37688759898770424 36-37 0.5795801812561883 38-39 0.5615868378206462 40-41 0.5224877888417928 42-43 0.5495264346530397 44-45 0.572091059934368 46-47 0.5587662596604802 48-49 0.5845405083654458 50-51 0.5755924673056086 52-53 0.5793856586244527 54-55 0.5691732204583342 56-57 0.5369797249060942 58-59 0.5349372372728706 60-61 0.47998459380756653 62-63 0.5580854304494056 64-65 0.5917378457396626 66-67 0.6054516912770216 68-69 0.6050626460135504 70-71 0.6026311131168556 72-73 0.5927104588983405 74-75 0.5916405844237949 76-77 0.5748143767786663 78-79 0.5483592988626261 80-81 0.5442743235961788 82-83 0.5589607822922158 84-85 0.5711184467756901 86-87 0.558377214397009 88-89 0.5730636730930461 90-91 0.5876528704732151 92-93 0.5962118662695811 94-95 0.5944611625839609 96-97 0.5912515391603236 98-99 0.5966981728489201 100-101 0.5706321401963511 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 514079.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.51437941291744 #Duplication Level Percentage of deduplicated Percentage of total 1 78.81682315471976 26.414969153280943 2 10.42613703431577 6.988510247582572 3 3.3234784092641507 3.3415294913615425 4 1.6192948248511103 2.1707864456573525 5 1.0020646255856156 1.679178702906969 6 0.6869818958305877 1.3814263144002985 7 0.5022634051278767 1.1783132427275669 8 0.362810438361953 0.9727493348983512 9 0.32607228306319946 0.9835299189554652 >10 2.386506689743411 16.441315492307503 >50 0.3284240871679235 7.581923679204723 >100 0.19298548130929308 11.257069243278751 >500 0.009300505123339426 2.1739172727008786 >1k 0.015113320825426569 12.7446606308891 >5k 0.0011625631404174283 2.5015925888298223 >10k+ 5.812815702087141E-4 2.1885282410181626 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 11234 2.18526724491761 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 7566 1.4717582317114686 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5275 1.0261068824052335 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4405 0.8568721927952707 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4074 0.7924852016907907 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4020 0.7819809795770689 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3596 0.699503383721179 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3554 0.6913334331882842 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3513 0.6833580052871251 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 3160 0.6146915162844621 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3159 0.6144969936527266 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3088 0.6006858867994996 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 3042 0.5917378457396626 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2771 0.5390222125393178 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2697 0.5246275377908843 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2356 0.45829532036904835 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2230 0.4337854687703641 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 2179 0.4238648145518491 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 2143 0.41686199980936783 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2083 0.4051906419052325 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1832 0.35636546133959957 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1819 0.3538366671270369 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1658 0.322518523417607 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 1579 0.3071512355104955 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 1511 0.2939236965524754 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1482 0.2882825402321433 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 1247 0.24256972177427982 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 1206 0.23459429387312067 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 1016 0.1976349938433587 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 994 0.19335549594517576 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 837 0.16281544276268822 No Hit GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 809 0.15736880907409173 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 796 0.15484001486152907 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 748 0.14550292853822078 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 738 0.1435577022208649 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 735 0.14297413432565811 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 735 0.14297413432565811 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 685 0.13324800273887866 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 661 0.12857945957722453 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 635 0.12352187115209919 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 579 0.11262860377490619 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 574 0.11165599061622825 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 551 0.10718197008630968 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 545 0.10601483429589614 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 537 0.10445865324201144 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 9.726131586779464E-5 0.0 12-13 0.0 0.0 0.0 4.863065793389732E-4 0.0 14-15 0.0 0.0 0.0 5.835678952067679E-4 0.0 16-17 0.0 0.0 0.0 5.835678952067679E-4 0.0 18-19 0.0 0.0 0.0 0.0011671357904135355 0.0 20-21 0.0 0.0 0.0 0.0014589197380169196 0.0 22-23 0.0 0.0 0.0 0.0029178394760338393 0.0 24-25 0.0 0.0 0.0 0.006127462899671062 0.0 26-27 0.0 0.0 0.0 0.01381110685322684 0.0 28-29 0.0 0.0 0.0 0.03919631029472124 0.0 30-31 0.0 0.0 0.0 0.0960941800773811 0.0 32-33 0.0 0.0 0.0 0.18061426356649463 0.0 34-35 0.0 0.0 0.0 0.30909646182785133 0.0 36-37 0.0 0.0 0.0 0.4771640156474005 0.0 38-39 9.726131586779464E-5 0.0 0.0 0.7028102684606841 0.0 40-41 1.9452263173558927E-4 0.0 0.0 0.9365292104909946 0.0 42-43 1.9452263173558927E-4 0.0 0.0 1.186393530955359 0.0 44-45 1.9452263173558927E-4 0.0 0.0 1.4700075280258482 0.0 46-47 1.9452263173558927E-4 0.0 0.0 1.8012795698715567 0.0 48-49 1.9452263173558927E-4 0.0 0.0 2.0984128898476695 0.0 50-51 1.9452263173558927E-4 0.0 0.0 2.4204451066859374 0.0 52-53 1.9452263173558927E-4 0.0 0.0 2.73168131746288 0.0 54-55 1.9452263173558927E-4 0.0 0.0 3.0662602440480935 0.0 56-57 1.9452263173558927E-4 0.0 0.0 3.5034498588738305 0.0 58-59 1.9452263173558927E-4 0.0 0.0 3.9207009039466696 0.0 60-61 1.9452263173558927E-4 0.0 0.0 4.382011325107619 0.0 62-63 1.9452263173558927E-4 0.0 0.0 4.8470176762715464 0.0 64-65 1.9452263173558927E-4 0.0 0.0 5.308036313484893 0.0 66-67 1.9452263173558927E-4 0.0 0.0 5.828676137325197 0.0 68-69 1.9452263173558927E-4 0.0 0.0 6.432474386232466 0.0 70-71 1.9452263173558927E-4 0.0 0.0 7.073426457801233 0.0 72-73 1.9452263173558927E-4 0.0 0.0 7.6957043567233825 0.0 74-75 1.9452263173558927E-4 0.0 0.0 8.419231285463908 0.0 76-77 1.9452263173558927E-4 0.0 0.0 9.098212531536982 0.0 78-79 1.9452263173558927E-4 0.0 0.0 9.96802047934267 0.0 80-81 1.9452263173558927E-4 0.0 0.0 10.864575289012 0.0 82-83 1.9452263173558927E-4 0.0 0.0 11.68351556861883 0.0 84-85 1.9452263173558927E-4 0.0 0.0 12.577152538812129 0.0 86-87 1.9452263173558927E-4 0.0 0.0 13.519809212202794 0.0 88-89 1.9452263173558927E-4 0.0 0.0 14.498549833780412 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTTCGCA 25 0.0050843223 56.81607 9 TGTTCGC 25 0.0050843223 56.81607 8 CGGATTA 35 2.8313202E-4 54.11055 9 GTAAGCG 35 7.841845E-8 47.559834 94-95 CGCAGTA 50 0.0016392587 37.88476 2 GCTTGCG 50 0.0016408287 37.87738 9 CATTCCG 95 8.909901E-7 34.887062 9 GTCCTAT 60 0.003977583 31.619925 1 GCTTATG 85 6.4578303E-4 27.899935 1 GTAGCGG 35 0.008335794 27.179707 66-67 CGCAGTC 35 0.008524486 27.055275 12-13 AGTGGAC 110 9.676902E-5 25.825487 8 CTTTGCG 405 0.0 25.724222 2 CCTTTAG 130 1.0122267E-5 25.539171 1 TAGGCAT 140 1.8192528E-5 23.677977 5 TTCTGCG 100 0.0016818717 23.677977 3 CGCAGAA 180 2.0083644E-7 23.673365 9 ACTCCCG 120 1.7468803E-4 23.673363 6 GCTTTGC 470 0.0 23.210371 1 TTTGCGT 450 0.0 23.151798 3 >>END_MODULE