##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938924_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 514079 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.053670739322165 33.0 31.0 34.0 30.0 34.0 2 32.286508493830716 34.0 31.0 34.0 30.0 34.0 3 32.3679434483805 34.0 31.0 34.0 30.0 34.0 4 35.94503179472416 37.0 35.0 37.0 35.0 37.0 5 35.8658980429078 37.0 35.0 37.0 35.0 37.0 6 35.933765043894034 37.0 35.0 37.0 35.0 37.0 7 35.89666568756942 37.0 35.0 37.0 35.0 37.0 8 35.883576259679934 37.0 35.0 37.0 35.0 37.0 9 37.50364243627925 39.0 37.0 39.0 34.0 39.0 10-11 37.43106020669975 39.0 37.0 39.0 34.0 39.0 12-13 37.3983891580866 39.0 37.0 39.0 34.0 39.0 14-15 38.51257977859434 40.0 38.0 41.0 34.0 41.0 16-17 38.51989188432128 40.0 38.0 41.0 34.0 41.0 18-19 38.66504758996186 40.0 38.0 41.0 34.0 41.0 20-21 38.66266079727046 40.0 38.0 41.0 34.0 41.0 22-23 38.59210743873996 40.0 38.0 41.0 34.0 41.0 24-25 38.483480165499856 40.0 38.0 41.0 34.0 41.0 26-27 38.274555078110566 40.0 37.5 41.0 34.0 41.0 28-29 38.101572910000215 40.0 37.0 41.0 33.0 41.0 30-31 37.9472785311207 40.0 37.0 41.0 33.0 41.0 32-33 37.72440033535702 40.0 36.5 41.0 33.0 41.0 34-35 37.60828880386089 40.0 36.0 41.0 33.0 41.0 36-37 37.44875884834821 40.0 36.0 41.0 32.5 41.0 38-39 37.27047788374938 40.0 36.0 41.0 32.0 41.0 40-41 37.18167538452261 40.0 36.0 41.0 32.0 41.0 42-43 36.992212286438466 40.0 36.0 41.0 31.0 41.0 44-45 36.737371104441145 40.0 35.0 41.0 31.0 41.0 46-47 36.388170884241525 40.0 35.0 41.0 30.0 41.0 48-49 36.05260767313973 39.0 35.0 41.0 29.5 41.0 50-51 35.798082590418986 39.0 35.0 41.0 28.0 41.0 52-53 35.651506869566745 39.0 35.0 41.0 28.0 41.0 54-55 35.47634215752832 39.0 35.0 40.0 27.0 41.0 56-57 35.16756082236388 38.5 35.0 40.0 26.5 41.0 58-59 34.90831662059722 38.0 34.5 40.0 26.0 41.0 60-61 34.41643502263271 38.0 34.0 40.0 24.0 41.0 62-63 34.14404498141336 38.0 34.0 40.0 23.5 41.0 64-65 33.90264434065581 37.5 34.0 40.0 23.0 41.0 66-67 33.38800067693876 37.0 33.0 39.0 22.0 41.0 68-69 32.91748155439144 36.0 32.5 39.0 21.0 40.0 70-71 32.36261644611042 35.5 32.0 38.5 19.0 40.0 72-73 31.80863544319064 35.0 31.5 37.5 16.5 40.0 74-75 31.33481721680909 35.0 31.0 37.0 12.5 39.0 76-77 29.300571507492037 33.0 28.5 35.0 7.5 37.5 78-79 30.269438160282757 34.0 30.0 36.0 7.0 38.0 80-81 30.203881115548388 34.0 30.0 36.0 4.0 37.5 82-83 29.842678848970685 34.0 30.0 35.0 2.0 37.0 84-85 29.39952322502962 34.0 29.5 35.0 2.0 36.5 86-87 28.90441449660461 34.0 29.0 35.0 2.0 36.0 88-89 28.299064929709246 34.0 29.0 35.0 2.0 36.0 90-91 27.799227356106748 33.0 27.0 35.0 2.0 35.0 92-93 27.46009757255208 33.0 27.0 35.0 2.0 35.0 94-95 27.047244684182782 33.0 26.0 35.0 2.0 35.0 96-97 26.418687594708206 33.0 25.0 34.5 2.0 35.0 98-99 25.584112558575626 32.5 22.5 34.0 2.0 35.0 100-101 21.335343400527933 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.11718075329545741 1101 2 0.18052200795911233 1101 3 0.10443363542140816 1101 4 0.0037986607146649476 1101 5 0.08226188048626426 1101 6 0.13241241245666657 1101 7 0.22680815263942833 1101 8 0.09423682546609768 1101 9 0.1735650187227833 1101 10-11 0.12520914759227963 1101 12-13 0.12963908339133212 1101 14-15 0.039055020087076286 1101 16-17 0.2320855094726113 1101 18-19 0.09944193783808686 1101 20-21 0.25387995403104924 1101 22-23 0.19188056610047255 1101 24-25 0.1383998113767433 1101 26-27 0.24171234872063252 1101 28-29 0.15460098104246356 1101 30-31 0.20884807602191557 1101 32-33 0.4156647086473271 1101 34-35 0.5055521184186134 1101 36-37 0.35448337225204796 1101 38-39 0.4887923888633168 1101 40-41 0.7087955012054366 1101 42-43 0.20440112175888459 1101 44-45 0.15388611621258264 1101 46-47 0.13542871139011226 1101 48-49 0.23198215182780046 1101 50-51 0.5238878693823636 1101 52-53 0.4085075659330002 1101 54-55 0.389343574479426 1101 56-57 0.31837132013291836 1101 58-59 0.3587173076241825 1101 60-61 0.356818140398083 1101 62-63 0.6103123387103722 1101 64-65 0.44731884904614816 1101 66-67 0.4434762894997917 1101 68-69 0.32282093732708717 1101 70-71 0.32236397126262517 1101 72-73 0.2582114542729137 1101 74-75 0.2770579690176582 1101 76-77 0.3712631513134532 1101 78-79 0.6132260494143686 1101 80-81 0.5952185422818737 1101 82-83 0.5851602230625694 1101 84-85 0.5795228649583883 1101 86-87 0.841860684674856 1101 88-89 1.1537549561107134 1101 90-91 1.1280743647348253 1101 92-93 1.0775933248377463 1101 94-95 0.9457985812877041 1101 96-97 0.7958268889438855 1101 98-99 0.9367951129673706 1101 100-101 1.064328418433444 1102 1 0.07046523031078067 1102 2 0.15843006925158676 1102 3 0.2760525561409324 1102 4 -0.008757413013960047 1102 5 2.0991512982959648E-4 1102 6 0.10274330297409762 1102 7 0.13597981597498432 1102 8 0.188991433505187 1102 9 0.17206379731483423 1102 10-11 0.05241767524051255 1102 12-13 0.24741834166358956 1102 14-15 0.287338386731534 1102 16-17 0.2672909280824243 1102 18-19 0.26305952611257055 1102 20-21 0.32243869264975444 1102 22-23 0.3428221605930375 1102 24-25 0.4454417833953954 1102 26-27 0.35004235094137215 1102 28-29 0.18979307520191213 1102 30-31 0.20000508246207005 1102 32-33 0.014272303584036194 1102 34-35 0.17789722168310362 1102 36-37 0.2980387797581514 1102 38-39 0.16925208240095913 1102 40-41 0.15109618963536775 1102 42-43 -0.1844818451520709 1102 44-45 0.04563828143577098 1102 46-47 0.4290063263134982 1102 48-49 0.22611939366657907 1102 50-51 0.24009484251138957 1102 52-53 0.11051511645488432 1102 54-55 0.03285900863443203 1102 56-57 0.2594594835795121 1102 58-59 -0.045992885580346865 1102 60-61 -0.24585119115865695 1102 62-63 -0.22920131885125272 1102 64-65 -0.08601004280373559 1102 66-67 -0.17192233364033882 1102 68-69 -0.05768095030337861 1102 70-71 0.10407838387643409 1102 72-73 -0.06194621838975678 1102 74-75 0.16097757816711322 1102 76-77 -0.46813280693660175 1102 78-79 -0.4634450587357506 1102 80-81 -0.12757737155335036 1102 82-83 -0.07368499123900563 1102 84-85 -0.10783889386906154 1102 86-87 2.9284101507442983E-4 1102 88-89 0.12219599541784021 1102 90-91 -0.016096188229862918 1102 92-93 -0.08154724811361902 1102 94-95 -0.14060603785508974 1102 96-97 -0.22161259584403226 1102 98-99 -0.39710450950653353 1102 100-101 0.426340410438776 1103 1 0.025839830332841984 1103 2 -0.09721596407071331 1103 3 -0.0935601548819136 1103 4 -0.019036570025981803 1103 5 0.019528021717057698 1103 6 -0.09249006346085764 1103 7 0.0036836667970092662 1103 8 0.06703623103359746 1103 9 -0.040563368082942475 1103 10-11 0.05345265262106835 1103 12-13 0.06744923651076817 1103 14-15 0.19377996236873685 1103 16-17 0.16325473697573045 1103 18-19 0.1322617770228689 1103 20-21 0.15156591321689916 1103 22-23 0.17949434419669785 1103 24-25 0.0535671282039587 1103 26-27 0.2026562756903516 1103 28-29 0.08782683885633702 1103 30-31 0.12058546423411087 1103 32-33 0.2857188444645331 1103 34-35 0.2919080348796115 1103 36-37 0.13344868817700473 1103 38-39 0.22601474377136554 1103 40-41 0.1915596123430845 1103 42-43 0.3885557803582529 1103 44-45 0.3850115573929571 1103 46-47 0.2032444373142681 1103 48-49 0.34654309442791487 1103 50-51 0.35217914130868877 1103 52-53 0.2698508772404935 1103 54-55 0.5455479637265626 1103 56-57 0.2744523115614186 1103 58-59 0.3247298331662023 1103 60-61 -0.01698437001775943 1103 62-63 0.30463736591097046 1103 64-65 0.44628389959944315 1103 66-67 0.4949583901818002 1103 68-69 0.48694003612495607 1103 70-71 0.17025293976301015 1103 72-73 0.6613043608886251 1103 74-75 0.21853688523982484 1103 76-77 0.21583497415133834 1103 78-79 0.21017294854660307 1103 80-81 0.4663208961284333 1103 82-83 0.6703710608408819 1103 84-85 0.6057482185661165 1103 86-87 0.622292178982633 1103 88-89 0.8393492559276083 1103 90-91 0.9468520629011081 1103 92-93 0.862131445475697 1103 94-95 0.5660782830099649 1103 96-97 0.830729895604712 1103 98-99 1.0179125929981545 1103 100-101 1.4319743064996793 1104 1 0.0070619368009303685 1104 2 0.022229444991552327 1104 3 -0.04120069969805229 1104 4 0.0560235578180297 1104 5 0.06667854068144408 1104 6 -0.005404366813863248 1104 7 -0.10274583250203762 1104 8 -0.013888773146412348 1104 9 -0.13665216436852035 1104 10-11 0.027787387865629398 1104 12-13 -0.0019003538481925375 1104 14-15 -0.0069184209317967316 1104 16-17 6.716190737066086E-4 1104 18-19 -0.09529156634248892 1104 20-21 0.08615196355707866 1104 22-23 0.1130643304348169 1104 24-25 0.1776105488673565 1104 26-27 0.09416892504534502 1104 28-29 -0.2191338433213872 1104 30-31 0.03501100216931974 1104 32-33 0.006828540802843008 1104 34-35 0.12100991138281358 1104 36-37 0.03512197855268795 1104 38-39 0.04150264746392196 1104 40-41 0.07871861994426865 1104 42-43 -0.06613290531782212 1104 44-45 0.024508155507447782 1104 46-47 0.23408005357644157 1104 48-49 -0.1428075248567282 1104 50-51 0.21811012611917846 1104 52-53 0.23325970799508156 1104 54-55 0.2575388062880748 1104 56-57 0.177784744584784 1104 58-59 -0.017683769231197743 1104 60-61 0.17170773683617568 1104 62-63 0.23396303374803296 1104 64-65 0.1569950158551805 1104 66-67 0.4031589277954595 1104 68-69 0.455589957026298 1104 70-71 0.06411282580949518 1104 72-73 0.24977910719413998 1104 74-75 0.5141279387311002 1104 76-77 0.11217071259843081 1104 78-79 0.136167469517396 1104 80-81 0.5207382026582543 1104 82-83 0.7379779083412323 1104 84-85 0.15426206404720588 1104 86-87 -0.03374439932504103 1104 88-89 0.5558866638066036 1104 90-91 0.5131148762411293 1104 92-93 0.4214039950249031 1104 94-95 0.1909255518553863 1104 96-97 0.2544029030258379 1104 98-99 0.6678077711952213 1104 100-101 0.9034602037871764 1105 1 0.009471664231249122 1105 2 -0.08869998032124471 1105 3 0.014202540319814716 1105 4 0.06170772779397282 1105 5 0.06749817586182161 1105 6 0.06964552847773575 1105 7 0.17753661378112184 1105 8 0.04255540355053711 1105 9 0.02361110245141873 1105 10-11 0.0823259127681979 1105 12-13 -0.0935499106304718 1105 14-15 -0.005303739626448589 1105 16-17 0.10195393831341448 1105 18-19 0.019199173829058225 1105 20-21 0.15395013659026802 1105 22-23 0.10056079575810628 1105 24-25 0.11259503727157494 1105 26-27 0.3447559887432945 1105 28-29 0.1393827809291608 1105 30-31 0.23788505292932172 1105 32-33 0.1670979548866427 1105 34-35 0.24723168007356833 1105 36-37 0.0723600538853546 1105 38-39 0.15804862285027355 1105 40-41 0.07216358758917352 1105 42-43 -0.08013842146258554 1105 44-45 -0.32295363924770015 1105 46-47 -0.47833045250730066 1105 48-49 -0.4145514216482695 1105 50-51 -0.19363155765737616 1105 52-53 -0.3603508259562602 1105 54-55 -0.39673702553918133 1105 56-57 -0.4683582571651357 1105 58-59 -0.3986645446880388 1105 60-61 -0.24678569173877207 1105 62-63 -0.23883532370306426 1105 64-65 -0.3929228157179878 1105 66-67 -0.3097474575724135 1105 68-69 -0.035816168107409396 1105 70-71 0.027491525950061657 1105 72-73 0.12319669902401742 1105 74-75 0.09378213464849594 1105 76-77 -0.1624583000280495 1105 78-79 0.024108996743628097 1105 80-81 -0.09656798435790748 1105 82-83 -0.30519432569739635 1105 84-85 -0.40729253799942455 1105 86-87 0.011964605596865852 1105 88-89 -0.2906591886344714 1105 90-91 -0.04732707104813372 1105 92-93 -0.023196863133129852 1105 94-95 0.2282537870578949 1105 96-97 0.4218912539608368 1105 98-99 0.5098251392646915 1105 100-101 1.0711268253739092 1106 1 -0.13694969253851497 1106 2 -0.283028396189124 1106 3 -0.0532858501156781 1106 4 -0.06877009532213663 1106 5 -0.11129716582556881 1106 6 -0.11904912991535355 1106 7 -0.06926220473945222 1106 8 -0.19652114311709568 1106 9 -0.09574004965197958 1106 10-11 -0.11138278977745841 1106 12-13 0.019565879182088963 1106 14-15 -0.09562293222852247 1106 16-17 -0.12967800026253684 1106 18-19 -0.21634017796690586 1106 20-21 -0.2007995233722326 1106 22-23 -0.08877369651963107 1106 24-25 -0.08794862032475237 1106 26-27 -0.05053258036279118 1106 28-29 -0.11710610044804781 1106 30-31 -0.2626582022557429 1106 32-33 -0.36520560806255276 1106 34-35 -0.47590535705289483 1106 36-37 -0.5822000703792369 1106 38-39 -0.40329494782520925 1106 40-41 -0.31283532590045837 1106 42-43 -0.19071097218651545 1106 44-45 -0.3993775714494632 1106 46-47 -0.3559672522111299 1106 48-49 -0.3580488582886119 1106 50-51 -0.22960712324638166 1106 52-53 -0.5053758156947694 1106 54-55 -0.5271705834179841 1106 56-57 -0.24005224755555332 1106 58-59 -0.4639658299895615 1106 60-61 -0.34585330487669097 1106 62-63 -0.5622894608931048 1106 64-65 -0.6871176310514571 1106 66-67 -0.5044038386075798 1106 68-69 -0.18866805662339203 1106 70-71 -0.26389244681474366 1106 72-73 -0.21226538981229126 1106 74-75 -0.20550519503094122 1106 76-77 -0.883985692161712 1106 78-79 -0.6900884745040905 1106 80-81 -0.4778204491267992 1106 82-83 -0.366316570186374 1106 84-85 -0.3861880800876172 1106 86-87 -0.5394112384607226 1106 88-89 -0.48333771838473893 1106 90-91 -0.19129170714764143 1106 92-93 -0.36859649774372016 1106 94-95 -0.2971987243907037 1106 96-97 -0.023068947566716957 1106 98-99 0.1346752410747385 1106 100-101 0.4015507253827586 1107 1 -0.06685608855057978 1107 2 -0.07968584309737992 1107 3 -0.18202649362356027 1107 4 -0.01092001796993003 1107 5 -0.03444501012103984 1107 6 6.749024006822424E-4 1107 7 0.023036952980923786 1107 8 0.10267564403149976 1107 9 -0.16791981931429234 1107 10-11 0.10081620073712827 1107 12-13 -0.06334553300361989 1107 14-15 -0.04482831406716059 1107 16-17 -0.009348432966113762 1107 18-19 -0.0897911804202991 1107 20-21 -0.0036764005306650915 1107 22-23 -0.12837177755974238 1107 24-25 -0.025418343575729807 1107 26-27 -0.14784009837257628 1107 28-29 0.005086951917050442 1107 30-31 -0.16764390381685956 1107 32-33 -0.014210186688345061 1107 34-35 -0.20445379203796676 1107 36-37 -0.4829626537867924 1107 38-39 -0.1897403402900082 1107 40-41 -0.08942807823274279 1107 42-43 0.10950545773550147 1107 44-45 -0.03620894363078975 1107 46-47 -0.47843377198672954 1107 48-49 -0.40010423934592154 1107 50-51 -0.7486170149532896 1107 52-53 -0.4812911501844823 1107 54-55 -0.6091131396350704 1107 56-57 -0.4254514318413385 1107 58-59 -0.5060563711425061 1107 60-61 -0.7282349716214966 1107 62-63 -0.8172353143458295 1107 64-65 -0.6708697601905911 1107 66-67 -0.732737406341549 1107 68-69 -0.9116846365618301 1107 70-71 -0.8058970410672437 1107 72-73 -0.7670721655533832 1107 74-75 -0.5508164009440897 1107 76-77 -0.9410388073461853 1107 78-79 -0.7462118004928691 1107 80-81 -0.5830350819772683 1107 82-83 -0.9820753250501859 1107 84-85 -1.0768456520095455 1107 86-87 -1.1567561043054262 1107 88-89 -1.0060120733923164 1107 90-91 -0.5328993829871855 1107 92-93 -1.0598986714189635 1107 94-95 -0.8872515426677055 1107 96-97 -0.8945431713099374 1107 98-99 -0.7715566905427842 1107 100-101 0.15523862874719185 1108 1 0.046258948477159834 1108 2 -0.022826297471674195 1108 3 0.0205983965484009 1108 4 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1999.0 14 2311.0 15 3168.0 16 4007.0 17 5712.0 18 5533.0 19 3721.0 20 3304.0 21 3584.0 22 3826.0 23 4256.0 24 4792.0 25 5517.0 26 6176.0 27 7442.0 28 8798.0 29 10389.0 30 12348.0 31 15247.0 32 18910.0 33 23445.0 34 31828.0 35 45989.0 36 65510.0 37 110646.0 38 94101.0 39 7347.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.19140476241625 23.289477024749488 16.796178505656663 18.722939707177606 2 13.738549911589464 25.4972484773741 38.169230799157326 22.594970811879108 3 20.76723616409151 28.363733978629746 33.169804640920944 17.699225216357796 4 10.940536376704747 20.203315054690037 42.14002128077591 26.7161272878293 5 9.888070589059163 41.28938635293751 36.71105716297654 12.111485895026785 6 24.317079670634282 41.50257061657839 17.664794710540598 16.51555500224674 7 21.767666059107647 36.84842212967268 24.18558237158102 17.198329439638655 8 22.784241332557837 32.40319094925099 22.088433878839634 22.72413383935154 9 21.937095271349342 21.058631066431424 26.237018045864545 30.76725561635468 10-11 22.302019728485313 27.53915254270258 29.418921994479447 20.739905734332662 12-13 26.23730982981215 26.90539780850803 28.455062354229604 18.402230007450214 14-15 21.702289074011937 29.207845196932343 30.426253106322555 18.663612622733176 16-17 17.23084464753101 32.19826617762488 31.088973897345966 19.481915277498143 18-19 17.10418048587863 32.89980722807195 31.995860558396664 18.000151727652757 20-21 19.39429542930172 29.556741279064113 30.401261284744173 20.647702006889993 22-23 18.10879261747708 29.519392933770877 31.854734389072497 20.517080059679543 24-25 20.705280705883727 30.26198308042149 30.842244090888755 18.190492122806027 26-27 19.642895352659806 28.129334207388357 34.522612283326104 17.705158156625732 28-29 17.16506606961187 31.88060589909333 32.69030635369272 18.26402167760208 30-31 18.344651308456484 29.775871023714256 31.808048957455952 20.07142871037331 32-33 18.518749063859836 30.03701765681928 32.89494416227856 18.54928911704232 34-35 20.32713666422535 29.080867942182575 31.823120160523978 18.768875233068094 36-37 19.439999766553086 28.541024890304268 33.88092845727581 18.13804688586683 38-39 18.74223025487682 30.139704637820447 32.8666784692216 18.251386638081136 40-41 18.117013350555673 30.555128671500075 32.96709923530476 18.36075874263949 42-43 17.65508011508093 30.958443645163236 30.755748697169466 20.630727542586364 44-45 18.861079473889486 29.01748676505059 31.135856767842014 20.985576993217908 46-47 20.646575033360694 30.506168324651124 30.78735133578951 18.05990530619867 48-49 19.402521993279223 29.684823786296814 31.458875364123113 19.45377885630085 50-51 16.68983118271308 30.536213968154545 33.60200281475617 19.171952034376208 52-53 18.191853781228176 32.08057516451752 30.28989707807555 19.43767397617876 54-55 17.417167400341192 29.556157711168908 31.409763868977336 21.616911019512568 56-57 19.795595618572243 29.623170757801816 29.965044283077113 20.616189340548825 58-59 21.04546493476178 28.99543356789589 30.42352563572613 19.5355758616162 60-61 19.16539656015928 30.416416616980946 31.977796560548338 18.44039026231144 62-63 18.03613841452384 30.40145580737591 31.879146979355312 19.68325879874494 64-65 17.82586621433407 32.15273617130541 30.55277818843906 19.46861942592146 66-67 18.774826192338068 30.02330403794006 29.049806059049864 22.152063710672003 68-69 17.64447850791619 29.907036549283855 30.8569989087153 21.591486034084653 70-71 20.10887431698241 29.944716668060746 29.389451815771505 20.556957199185337 72-73 19.122061006187767 27.952318612508975 32.149144392204306 20.77647598909895 74-75 17.530379572011306 32.17345972117126 29.560048163803614 20.736112543013817 76-77 18.451262845362912 28.99991917633873 30.253464949309933 22.29535302898843 78-79 18.459729399303804 28.97310430216397 31.129292510774132 21.437873787758097 80-81 19.61537040026922 28.981926902285448 29.96757307728968 21.435129620155656 82-83 18.218017075196613 28.829810204268213 31.599228912287803 21.35294380824737 84-85 18.044600149004335 29.11643930213061 30.562423285137108 22.27653726372795 86-87 17.77155697633985 29.69260604502159 30.47913482807893 22.05670215055963 88-89 18.237636663596323 28.915101296869523 29.305910587600962 23.541351451933192 90-91 18.537649813163554 27.266703295523637 30.587405203314553 23.60824168799826 92-93 19.387487137190977 29.122761287662012 30.44288912793559 21.04686244721142 94-95 18.394546363496662 27.9259607959088 30.697421991561608 22.982070849032933 96-97 16.501256623534264 28.079019444595744 31.803274224044692 23.6164497078253 98-99 17.986437882115393 28.720974791812154 29.245213284339567 24.047374041732887 100-101 17.59178568232836 26.91342558911294 30.947249250113796 24.547539478444904 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4499.0 1 2761.0 2 927.0 3 779.0 4 634.5 5 627.5 6 1002.0 7 1774.0 8 2410.5 9 2383.0 10 1787.5 11 1249.0 12 1117.0 13 1092.0 14 1044.5 15 983.0 16 940.0 17 911.0 18 929.0 19 1056.0 20 1199.5 21 1370.0 22 1558.5 23 1760.0 24 2008.0 25 2558.0 26 3771.5 27 5021.5 28 5449.0 29 5950.0 30 7476.5 31 9031.0 32 10841.5 33 13406.5 34 16411.0 35 18747.0 36 21268.0 37 23626.0 38 23369.0 39 22003.0 40 20093.5 41 17799.0 42 15834.0 43 15049.5 44 15144.5 45 14944.0 46 16048.0 47 25560.0 48 46191.0 49 43303.5 50 22025.5 51 13025.5 52 8225.5 53 4851.5 54 2852.0 55 2172.5 56 1726.5 57 1341.0 58 1081.0 59 847.0 60 649.0 61 492.0 62 347.5 63 210.0 64 138.0 65 119.5 66 91.5 67 71.0 68 66.0 69 57.0 70 55.5 71 53.5 72 40.5 73 28.0 74 18.5 75 16.0 76 11.0 77 6.5 78 3.5 79 1.5 80 2.0 81 2.0 82 0.5 83 0.0 84 0.0 85 0.0 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.39449189715977506 2 0.0 3 0.0 4 0.0 5 9.726131586779463E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0012643971062813303 16-17 0.002431532896694866 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.002431532896694866 36-37 0.008461734480498135 38-39 0.012838493694548894 40-41 0.0035014073712406075 42-43 0.0011671357904135358 44-45 8.753518428101519E-4 46-47 1.9452263173558927E-4 48-49 2.9178394760338394E-4 50-51 4.8630657933897316E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 2.9178394760338394E-4 60-61 0.0028205781601660447 62-63 0.0 64-65 5.835678952067679E-4 66-67 9.726131586779463E-4 68-69 0.0011671357904135358 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.1197286798332552 78-79 9.726131586779464E-5 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.14540566722235299 88-89 0.42649087008027947 90-91 0.33701045948190844 92-93 0.0 94-95 0.0 96-97 5.835678952067679E-4 98-99 0.0 100-101 1.9452263173558927E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 514079.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 31.8572512595771 #Duplication Level Percentage of deduplicated Percentage of total 1 77.43749831892532 24.669458408590835 2 11.024460964312045 7.024180458829771 3 3.560341072500949 3.4026804034936484 4 1.8107862290963088 2.30746687510813 5 1.0785064012152226 1.7179124704287814 6 0.7240197134294736 1.383916675656585 7 0.49935156807538444 1.113557785972894 8 0.4097733135831115 1.0443401128229328 9 0.29839694556630253 0.8555495822996445 >10 2.572910076782712 16.836786629881797 >50 0.34829205515303685 7.758944167026474 >100 0.20566982518703056 11.523657431713023 >500 0.01163013892426661 2.5426342203588477 >1k 0.017139152098919214 14.088716909786516 >5k 6.121125749614005E-4 1.4129862382636866 >10k+ 6.121125749614005E-4 2.3172116297664074 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 11883 2.3115124329140073 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 7246 1.4095109895560798 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4959 0.9646377307767873 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4444 0.8644585754329588 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3869 0.7526080621849949 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3795 0.7382133874365613 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3746 0.7286817784815174 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3547 0.6899717747661351 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3521 0.6849141863410099 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3447 0.6705195115925763 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 3343 0.650289157892075 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 3208 0.6240286026077704 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3085 0.6001023189042929 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2958 0.5753979446738731 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2826 0.5497209572847753 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2391 0.4651036124797939 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2313 0.449930847204418 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 2159 0.4199743619171373 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2152 0.41861270349498814 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 2024 0.39371380663283273 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1995 0.3880726503125006 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1851 0.36006139134257575 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1779 0.34605576185761333 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 1414 0.27505500127412325 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 1412 0.274665956010652 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1409 0.27408238811544533 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 1279 0.2487944459898187 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 1232 0.23965188229824597 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 1072 0.2085282612205517 No Hit CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT 1019 0.19821856173856547 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 909 0.17682107224765065 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 901 0.17526489119376593 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 826 0.16067569381359673 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 764 0.14861529064599022 No Hit GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 754 0.1466700643286343 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 722 0.14044534011309545 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 707 0.13752750063706162 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 705 0.13713845537359043 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 681 0.1324699122119363 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 677 0.13169182168499394 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 671 0.13052468589458038 No Hit ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT 657 0.12780136905028217 RNA PCR Primer, Index 27 (95% over 23bp) GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 637 0.12391091641557038 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 617 0.12002046378085858 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 614 0.11943689588565182 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 591 0.11496287535573327 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 553 0.10757101534978088 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 544 0.10582031166416056 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 9.726131586779464E-5 0.0 12-13 0.0 0.0 0.0 4.863065793389732E-4 0.0 14-15 0.0 0.0 0.0 5.835678952067679E-4 0.0 16-17 0.0 0.0 0.0 6.808292110745625E-4 0.0 18-19 0.0 0.0 0.0 0.0012643971062813303 0.0 20-21 0.0 9.726131586779464E-5 0.0 0.0014589197380169196 0.0 22-23 0.0 1.9452263173558927E-4 0.0 0.0029178394760338393 0.0 24-25 0.0 1.9452263173558927E-4 0.0 0.006516508163142241 0.0 26-27 0.0 1.9452263173558927E-4 0.0 0.014200152116698016 0.0 28-29 0.0 1.9452263173558927E-4 0.0 0.03968261687406021 0.0 30-31 0.0 1.9452263173558927E-4 0.0 0.09745583849953024 0.0 32-33 0.0 1.9452263173558927E-4 0.0 0.18246222856798272 0.0 34-35 0.0 1.9452263173558927E-4 0.0 0.31318143709429874 0.0 36-37 0.0 1.9452263173558927E-4 0.0 0.48387504644227836 0.0 38-39 0.0 1.9452263173558927E-4 0.0 0.7119528321522568 0.0 40-41 0.0 1.9452263173558927E-4 0.0 0.9491731815538079 0.0 42-43 0.0 3.8904526347117855E-4 0.0 1.2013717735989995 0.0 44-45 0.0 3.8904526347117855E-4 0.0 1.4912104948850273 0.0 46-47 0.0 3.8904526347117855E-4 0.0 1.8258866827861087 0.0 48-49 0.0 3.8904526347117855E-4 0.0 2.1258405809223873 0.0 50-51 0.0 3.8904526347117855E-4 0.0 2.4516659890794994 0.0 52-53 0.0 3.8904526347117855E-4 0.0 2.763583029067517 0.0 54-55 0.0 3.8904526347117855E-4 0.0 3.100788011181161 0.0 56-57 0.0 3.8904526347117855E-4 0.0 3.5390475004814435 0.0 58-59 0.0 3.8904526347117855E-4 0.0 3.9548396258162657 0.0 60-61 0.0 3.8904526347117855E-4 0.0 4.419359670400853 0.0 62-63 0.0 3.8904526347117855E-4 0.0 4.887867428936019 0.0 64-65 0.0 3.8904526347117855E-4 0.0 5.349664156676308 0.0 66-67 0.0 3.8904526347117855E-4 0.0 5.872443729465704 0.0 68-69 0.0 3.8904526347117855E-4 0.0 6.477798159426859 0.0 70-71 0.0 3.8904526347117855E-4 0.0 7.123321512841412 0.0 72-73 0.0 3.8904526347117855E-4 0.0 7.748030944660257 0.0 74-75 0.0 3.8904526347117855E-4 0.0 8.476809984457642 0.0 76-77 0.0 3.8904526347117855E-4 0.0 9.15656932105766 0.0 78-79 0.0 3.8904526347117855E-4 0.0 10.027349882022024 0.0 80-81 0.0 3.8904526347117855E-4 0.0 10.927892405641934 0.0 82-83 0.0 3.8904526347117855E-4 0.0 11.7497505247248 0.0 84-85 0.0 3.8904526347117855E-4 0.0 12.638135383861236 0.0 86-87 0.0 3.8904526347117855E-4 0.0 13.577387911196528 0.0 88-89 0.0 3.8904526347117855E-4 0.0 14.556614839353486 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATTCCG 115 0.0 49.513653 9 GACGTGG 60 8.340096E-5 39.538307 7 GTAAGAC 65 1.3360796E-4 36.4969 3 GTAAGCG 100 0.0 35.587936 94-95 CCTTTAG 170 3.6379788E-12 33.641792 1 TAGGCAT 175 3.6379788E-12 32.534378 5 TTAGGCA 180 5.456968E-12 31.630644 4 GGCATTC 180 5.456968E-12 31.630644 7 CTTTGCG 460 0.0 30.943024 2 CGCAGAA 145 2.4188921E-8 29.452087 9 GCTTGCG 65 0.0058708903 29.20036 9 GGACGTG 150 3.36604E-8 28.467579 6 TTTGCGT 500 0.0 28.467579 3 GCTTTGC 520 0.0 27.495697 1 GTTATAT 70 0.008251508 27.233833 1 CTGACCA 70 0.008430021 27.114618 9 GAGCGGC 35 0.008433875 27.114618 92-93 TTAGCAC 70 0.008434022 27.11198 3 TAGGACG 140 5.853508E-7 27.11198 4 TTGCGTT 540 0.0 26.358871 4 >>END_MODULE