##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938923_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 319108 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.702323351341864 33.0 31.0 34.0 30.0 34.0 2 31.7779403838199 33.0 31.0 34.0 30.0 34.0 3 31.79850708850922 34.0 31.0 34.0 30.0 34.0 4 35.300171101946674 37.0 35.0 37.0 33.0 37.0 5 35.33785426877421 37.0 35.0 37.0 33.0 37.0 6 35.455228323953015 37.0 35.0 37.0 33.0 37.0 7 35.415006831542925 37.0 35.0 37.0 33.0 37.0 8 35.4139194253983 37.0 35.0 37.0 33.0 37.0 9 36.99632099477293 39.0 37.0 39.0 33.0 39.0 10-11 36.901376649911626 39.0 37.0 39.0 33.0 39.0 12-13 36.84116819384033 39.0 37.0 39.0 33.0 39.0 14-15 37.98095002318964 40.0 38.0 41.0 33.0 41.0 16-17 37.90113378542688 40.0 38.0 41.0 33.0 41.0 18-19 37.83889466888953 40.0 38.0 41.0 33.0 41.0 20-21 37.93585400554045 40.0 38.0 41.0 33.0 41.0 22-23 37.87307588653371 40.0 38.0 41.0 33.0 41.0 24-25 37.77669315717563 40.0 38.0 41.0 33.0 41.0 26-27 37.61941725058601 40.0 37.5 41.0 32.5 41.0 28-29 37.51694410669742 40.0 37.0 41.0 32.0 41.0 30-31 37.375075209646894 40.0 37.0 41.0 32.0 41.0 32-33 37.14091310778796 40.0 36.5 41.0 31.5 41.0 34-35 36.83203022174311 40.0 36.0 41.0 30.5 41.0 36-37 36.547483923937975 39.5 35.5 41.0 30.0 41.0 38-39 36.29770641914337 39.0 35.0 41.0 30.0 41.0 40-41 36.22723654687441 39.0 35.0 41.0 30.0 41.0 42-43 36.02218997956805 39.0 35.0 41.0 30.0 41.0 44-45 35.91826278250623 39.0 35.0 41.0 29.0 41.0 46-47 35.66358411572257 39.0 35.0 41.0 27.0 41.0 48-49 35.39717744462689 39.0 35.0 41.0 26.0 41.0 50-51 34.29697312508618 38.0 34.0 40.0 23.5 40.5 52-53 34.457204144051545 38.5 34.5 39.5 24.0 40.5 54-55 34.99896116675232 39.0 35.0 40.5 24.0 41.0 56-57 34.91002575930406 39.0 35.0 41.0 23.0 41.0 58-59 34.76371949308698 39.0 35.0 41.0 23.0 41.0 60-61 34.41528103338055 39.0 34.5 40.0 22.0 41.0 62-63 34.083535981548565 38.0 34.0 40.0 21.0 41.0 64-65 33.73593109542851 38.0 34.0 40.0 19.5 41.0 66-67 33.273114431477744 37.0 33.0 40.0 16.5 41.0 68-69 32.83557604322048 36.5 33.0 39.0 13.0 41.0 70-71 32.29566949120674 36.0 32.0 39.0 8.0 40.5 72-73 31.79511325319328 35.5 31.5 38.5 6.5 40.0 74-75 31.30658272434411 35.0 31.0 37.5 2.0 39.5 76-77 30.864752685611144 35.0 31.0 37.0 2.0 39.0 78-79 30.403667410406506 35.0 31.0 36.5 2.0 39.0 80-81 29.899574752121538 35.0 30.0 36.0 2.0 38.5 82-83 29.375365080160947 34.0 29.5 35.5 2.0 37.0 84-85 28.88709935194354 34.0 29.0 35.0 2.0 37.0 86-87 28.49222520275267 34.0 29.0 35.0 2.0 36.0 88-89 27.94197262368853 34.0 28.0 35.0 2.0 36.0 90-91 27.444004851022225 34.0 26.5 35.0 2.0 36.0 92-93 26.95822730862279 34.0 25.5 35.0 2.0 35.0 94-95 26.405763879313586 33.0 25.0 35.0 2.0 35.0 96-97 25.775254772678842 33.0 22.5 35.0 2.0 35.0 98-99 25.017932800180503 33.0 10.0 35.0 2.0 35.0 100-101 21.987696955262795 28.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.07264969999529214 1101 2 0.20726235522851155 1101 3 0.3931537179589206 1101 4 0.19329954438317287 1101 5 0.16386799920695694 1101 6 0.3490953042609348 1101 7 0.47225674164199205 1101 8 0.6276283642636358 1101 9 0.764327445686547 1101 10-11 0.5598888968028675 1101 12-13 0.5678032953164234 1101 14-15 0.6597269014527924 1101 16-17 0.7546722177986211 1101 18-19 0.7635546417531884 1101 20-21 0.5387802598347307 1101 22-23 0.7978523390364458 1101 24-25 0.8251002152637525 1101 26-27 0.718771738995315 1101 28-29 0.7529959950569634 1101 30-31 0.7489755700939966 1101 32-33 0.3246794866620135 1101 34-35 0.43057118786715876 1101 36-37 -0.10893557617342964 1101 38-39 0.117330501472658 1101 40-41 0.14316457304152408 1101 42-43 0.2083242852483167 1101 44-45 -0.010580783355635504 1101 46-47 -0.061459850479288036 1101 48-49 -0.04676859286115587 1101 50-51 0.2946432424095775 1101 52-53 0.5241334582215416 1101 54-55 0.23919943535999977 1101 56-57 0.4323593844424707 1101 58-59 0.4861153989534799 1101 60-61 0.7612834105755368 1101 62-63 0.4041775886161716 1101 64-65 0.5656199501669974 1101 66-67 0.8630549285958438 1101 68-69 0.3024733030269786 1101 70-71 0.24901931096545837 1101 72-73 -0.12374604040779857 1101 74-75 0.03791328626660828 1101 76-77 -0.3916722023402279 1101 78-79 -0.4581015647822788 1101 80-81 -0.27681214967083534 1101 82-83 -0.12844778823404823 1101 84-85 0.037216508167446705 1101 86-87 0.26109447893611915 1101 88-89 0.07796659273885354 1101 90-91 -0.10381863209715192 1101 92-93 0.07141934019501761 1101 94-95 -0.5144828506230219 1101 96-97 -0.5281188815805145 1101 98-99 -0.4119938968617838 1101 100-101 0.24525492114826974 1102 1 0.35319780889719965 1102 2 0.3560149866801048 1102 3 0.5224275583054272 1102 4 0.35784088539510606 1102 5 0.3454563070928458 1102 6 0.5761502562293828 1102 7 0.41106188113917597 1102 8 0.5544558620253923 1102 9 0.5382040880753038 1102 10-11 0.7745488043693456 1102 12-13 0.648113988083864 1102 14-15 0.8309950796413759 1102 16-17 0.8946714918809349 1102 18-19 0.8659090347650178 1102 20-21 0.7256314709073948 1102 22-23 0.7178380626553817 1102 24-25 0.8944193044790296 1102 26-27 0.8222452550985935 1102 28-29 1.0302784012321027 1102 30-31 0.7056201366001531 1102 32-33 0.8364454024071648 1102 34-35 0.5864559605339466 1102 36-37 0.3344428447136423 1102 38-39 0.3840205588201471 1102 40-41 0.6110185184041157 1102 42-43 0.47855924060438326 1102 44-45 0.7542158387074238 1102 46-47 0.9033089447393294 1102 48-49 0.628352998350401 1102 50-51 0.5917371519602384 1102 52-53 0.5543316337484328 1102 54-55 0.2322003790529763 1102 56-57 0.5455601974702731 1102 58-59 0.7749459455694918 1102 60-61 1.0025421518342839 1102 62-63 0.8706341424429667 1102 64-65 0.2727726590498136 1102 66-67 -0.00736271604325367 1102 68-69 0.15471275907266602 1102 70-71 0.6159525507360684 1102 72-73 0.600411290150916 1102 74-75 0.2084615960882772 1102 76-77 0.14267580259600976 1102 78-79 -0.03939829573298681 1102 80-81 0.4565945820057955 1102 82-83 0.6867880140259786 1102 84-85 0.33710520078970063 1102 86-87 0.5470999959104894 1102 88-89 0.36826107334475466 1102 90-91 0.3756137002763573 1102 92-93 0.05188005594985512 1102 94-95 0.5767529032424896 1102 96-97 0.7086936138824989 1102 98-99 0.7275274814139188 1102 100-101 0.860542747508763 1103 1 0.3627607799357335 1103 2 0.556505577759399 1103 3 0.5257574156311087 1103 4 0.5258505550163761 1103 5 0.28701197851532356 1103 6 0.730046544801219 1103 7 0.35797376434531714 1103 8 0.198540329011216 1103 9 0.5906997035656545 1103 10-11 0.6526061110954231 1103 12-13 0.5932128173012785 1103 14-15 0.608333089784999 1103 16-17 0.9273420137560819 1103 18-19 0.8206123101122245 1103 20-21 0.7048761502456884 1103 22-23 0.5692624357890068 1103 24-25 0.71402635761455 1103 26-27 0.6530446578572793 1103 28-29 0.6956988401570712 1103 30-31 0.8241144722876967 1103 32-33 0.7315311965959097 1103 34-35 0.4531787173240076 1103 36-37 0.996450476130434 1103 38-39 0.8370115054002341 1103 40-41 0.9613823142044637 1103 42-43 0.9881044366038125 1103 44-45 0.837201571276033 1103 46-47 0.8191441345767458 1103 48-49 0.5841227760439125 1103 50-51 0.6977948852872444 1103 52-53 0.8356216275864199 1103 54-55 0.9291122622591246 1103 56-57 0.7420514047509315 1103 58-59 0.7307868234194146 1103 60-61 0.9597339326561993 1103 62-63 0.6459991232459288 1103 64-65 0.5595729150103779 1103 66-67 0.8353104128376287 1103 68-69 0.6503993495831608 1103 70-71 0.5575378472237276 1103 72-73 0.5305790865200635 1103 74-75 0.7025602831053845 1103 76-77 0.756797478663696 1103 78-79 0.8826877828563866 1103 80-81 0.829040901387696 1103 82-83 0.7438340395272327 1103 84-85 0.8123386463032816 1103 86-87 0.7347629337689554 1103 88-89 1.0926590920203978 1103 90-91 0.8794412586969855 1103 92-93 0.2998655515179429 1103 94-95 0.1455660046313092 1103 96-97 0.307078455329151 1103 98-99 0.4806203382182055 1103 100-101 0.5625027418207473 1104 1 0.16246587887028596 1104 2 0.2384880739542261 1104 3 0.2527836713388716 1104 4 0.2663376174212502 1104 5 0.045763881102836024 1104 6 0.03108345499908438 1104 7 -0.14159815346290117 1104 8 0.44326064864592496 1104 9 0.21722177358088146 1104 10-11 0.3585849923739559 1104 12-13 0.37037225413537556 1104 14-15 0.2818252891761759 1104 16-17 0.36453284328424473 1104 18-19 0.31466672411527696 1104 20-21 0.37732192650140206 1104 22-23 0.4075187143278143 1104 24-25 0.39172803189156724 1104 26-27 0.6797760124995875 1104 28-29 0.5200900121509804 1104 30-31 0.3102493657427914 1104 32-33 0.42514325900078376 1104 34-35 0.2651792881002706 1104 36-37 0.026796747058895676 1104 38-39 0.35881991483706344 1104 40-41 0.06726130963825483 1104 42-43 0.16630554010457388 1104 44-45 -0.09211730239215399 1104 46-47 0.14703131113686396 1104 48-49 -0.029152528370090636 1104 50-51 0.17684264023397844 1104 52-53 0.3481549228680052 1104 54-55 0.6014153444509063 1104 56-57 0.7349404838985691 1104 58-59 0.48720805629613295 1104 60-61 0.5667782629453981 1104 62-63 0.5669228294864155 1104 64-65 0.34945590587795294 1104 66-67 0.11872460614052471 1104 68-69 0.39459645765013107 1104 70-71 0.5569908533120014 1104 72-73 0.39889624785948996 1104 74-75 0.12365046575378713 1104 76-77 0.31475505096202383 1104 78-79 0.5280216869659746 1104 80-81 0.7063002240665419 1104 82-83 0.6785039405926838 1104 84-85 0.8153827863337213 1104 86-87 0.727889836356475 1104 88-89 0.3537654466627096 1104 90-91 0.024204386550870538 1104 92-93 0.3766349579106354 1104 94-95 0.26935310508793364 1104 96-97 -0.10407070753234393 1104 98-99 -0.16761501185790095 1104 100-101 0.12041274943110025 1105 1 0.19488474575697623 1105 2 0.2730727824250714 1105 3 0.2887778957613172 1105 4 0.5325642400835164 1105 5 0.4324525499697245 1105 6 0.3177652481784037 1105 7 0.5606501963720802 1105 8 0.2882482724082962 1105 9 0.3903515865621756 1105 10-11 0.5061200583805672 1105 12-13 0.24664425633559972 1105 14-15 0.490244568604119 1105 16-17 0.6356583933392486 1105 18-19 0.4999321256554268 1105 20-21 0.5800343852472665 1105 22-23 0.3554869486512544 1105 24-25 0.33287214630301065 1105 26-27 0.209822767175055 1105 28-29 0.6047984829980635 1105 30-31 0.3281777210783261 1105 32-33 0.5043339905739401 1105 34-35 0.2940056082322826 1105 36-37 0.36886220360455013 1105 38-39 0.5097928246280432 1105 40-41 0.3764609796052554 1105 42-43 0.47033125517607743 1105 44-45 0.46845750508859396 1105 46-47 0.503346630168771 1105 48-49 0.32162786216895967 1105 50-51 0.5582542188918396 1105 52-53 0.5107821230880205 1105 54-55 0.589709486365102 1105 56-57 0.6921565180019797 1105 58-59 0.5477484853390351 1105 60-61 0.5652484224613445 1105 62-63 0.666224947198188 1105 64-65 0.7408028530726725 1105 66-67 0.7520957557554837 1105 68-69 0.7728280308074531 1105 70-71 0.6018719039170577 1105 72-73 0.5872385195866627 1105 74-75 0.6753212578329979 1105 76-77 0.433687256682596 1105 78-79 0.7268150037976575 1105 80-81 0.3532508566297956 1105 82-83 0.3465285555541797 1105 84-85 0.8060318512402134 1105 86-87 0.9763256174345827 1105 88-89 0.9555428118992317 1105 90-91 1.1260505086630772 1105 92-93 1.0785739018050258 1105 94-95 1.1507990621836406 1105 96-97 1.1808577853169417 1105 98-99 0.9955622433676226 1105 100-101 0.788609256581811 1106 1 0.04399365664806609 1106 2 0.050432518398672244 1106 3 0.21986700467220643 1106 4 0.3274487285323602 1106 5 0.4202083255472857 1106 6 0.4653890105546381 1106 7 0.23381851320376512 1106 8 0.17867731531259068 1106 9 0.3244439958031009 1106 10-11 0.3754599923739619 1106 12-13 0.4444000319131618 1106 14-15 0.5668947336206216 1106 16-17 0.5579356210620219 1106 18-19 0.6320278352263813 1106 20-21 0.4221135931680635 1106 22-23 0.5814770476611528 1106 24-25 0.44617247633601664 1106 26-27 0.5067204569440378 1106 28-29 0.3962011232620952 1106 30-31 0.13073547685390707 1106 32-33 0.46812937011188893 1106 34-35 0.34747095476693346 1106 36-37 0.45985230261445054 1106 38-39 0.5401393592815111 1106 40-41 0.5030946429715897 1106 42-43 0.4742916512156796 1106 44-45 0.34649380871895374 1106 46-47 0.7096007555813131 1106 48-49 0.7716113605187971 1106 50-51 0.6846898624561959 1106 52-53 0.8382243673124492 1106 54-55 0.7249570111175743 1106 56-57 0.5873710394541263 1106 58-59 0.615124722962797 1106 60-61 0.6158754851676136 1106 62-63 0.3378256072641932 1106 64-65 0.5729975725446224 1106 66-67 0.48115516169607986 1106 68-69 0.8648742354279122 1106 70-71 0.8252547422008902 1106 72-73 0.5734101367483788 1106 74-75 0.7557337990871247 1106 76-77 0.25718560651757727 1106 78-79 0.93198002029931 1106 80-81 0.9980363351776518 1106 82-83 0.9909344961482383 1106 84-85 1.4948272307781671 1106 86-87 1.136292614134252 1106 88-89 1.3346682244404846 1106 90-91 1.8198293865508681 1106 92-93 1.4858016245773058 1106 94-95 1.6028947717545954 1106 96-97 1.1031515146898805 1106 98-99 0.825232210364323 1106 100-101 0.9546488605422105 1107 1 0.13366685926244415 1107 2 0.2625893811437727 1107 3 0.4413049131689384 1107 4 0.36110885925131697 1107 5 0.372561266723757 1107 6 0.14362430467228648 1107 7 0.29610609490310935 1107 8 0.23880803426683883 1107 9 0.2883002049534298 1107 10-11 0.24532927341971345 1107 12-13 0.41387715609616293 1107 14-15 0.4559470212023129 1107 16-17 0.3474781047221498 1107 18-19 0.4929755476446829 1107 20-21 0.4962965997040101 1107 22-23 0.46235940060233105 1107 24-25 0.6433620188196727 1107 26-27 0.6491387575976262 1107 28-29 0.7079985088830014 1107 30-31 0.8027943003833187 1107 32-33 0.9597633570399964 1107 34-35 0.999758536466274 1107 36-37 0.807434001960857 1107 38-39 0.8623942612422937 1107 40-41 0.5578658848016573 1107 42-43 1.0612197557908445 1107 44-45 0.37397746884968086 1107 46-47 0.5380648078688921 1107 48-49 0.6271995958129182 1107 50-51 0.8117486859856129 1107 52-53 0.804139399992188 1107 54-55 1.0094014555620205 1107 56-57 0.8684167910881158 1107 58-59 0.8542423700216233 1107 60-61 0.9224767923571591 1107 62-63 0.7365510974602714 1107 64-65 0.874272082348547 1107 66-67 0.45890025973529447 1107 68-69 0.6499069151664756 1107 70-71 0.3469214088675585 1107 72-73 0.7838022936111173 1107 74-75 0.7043285703289541 1107 76-77 0.9253555411581011 1107 78-79 1.0795943993842734 1107 80-81 1.0724154201449707 1107 82-83 0.9631240386319 1107 84-85 0.6944677536539849 1107 86-87 0.9338743134806577 1107 88-89 0.7456159368587905 1107 90-91 0.8314960532175348 1107 92-93 1.272468291243971 1107 94-95 1.0000516344996946 1107 96-97 1.353445632336939 1107 98-99 1.2556897267041904 1107 100-101 1.403603108908225 1108 1 -0.10600634335193604 1108 2 -0.0029008149346623213 1108 3 -0.040132995327791576 1108 4 -0.06588460480097069 1108 5 -0.04312500778605255 1108 6 0.13872234388797722 1108 7 0.1404851798704314 1108 8 0.09534398197925498 1108 9 -0.04555600419689654 1108 10-11 0.11879332570729417 1108 12-13 0.2610666985798247 1108 14-15 0.48189473362061364 1108 16-17 0.2546022877286873 1108 18-19 0.263694501893049 1108 20-21 0.4371135931680641 1108 22-23 0.5198103809944854 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-1.5281155909397022 2316 96-97 -1.871917569938791 2316 98-99 -1.5711753889448303 2316 100-101 -1.1478209149327476 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2456.0 3 418.0 4 390.0 5 341.0 6 397.0 7 448.0 8 531.0 9 596.0 10 745.0 11 1016.0 12 1350.0 13 1990.0 14 1828.0 15 2025.0 16 2584.0 17 3215.0 18 2880.0 19 2139.0 20 2004.0 21 2024.0 22 2290.0 23 2607.0 24 2904.0 25 3283.0 26 3744.0 27 4433.0 28 5186.0 29 6180.0 30 7294.0 31 8872.0 32 10956.0 33 13850.0 34 18215.0 35 27301.0 36 37364.0 37 59477.0 38 64945.0 39 10830.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.67866597081182 22.119695416789803 16.790746807346718 19.410891805051655 2 14.364623554394296 24.43422592068655 38.19943477483525 23.001715750083907 3 20.618847753665467 26.89327636721813 32.750810888809426 19.737064990306976 4 11.83176532222393 19.73466679633214 41.05951331528473 27.374054566159195 5 11.131434380068834 39.409293555501876 36.381405359277394 13.077866705151898 6 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33.31671049763438 18.79289985368768 28-29 18.326903776188683 30.92303349642454 31.569282398695268 19.180780328691508 30-31 19.390566514088757 28.97804163981264 30.868408218821497 20.762983627277105 32-33 19.281952216910202 29.474549083741923 31.644027427374375 19.599471271973496 34-35 20.949538010758264 28.58061311426964 30.67127555559747 19.798573319374626 36-37 19.964411410394703 28.158605431196705 32.67347469037139 19.203508468037196 38-39 19.723551637279595 29.32147355163728 31.860358942065492 19.09461586901763 40-41 19.18489347234814 29.880471189684698 31.622406064269164 19.312229273697994 42-43 18.918701900671344 29.983751716146262 29.688228181325808 21.409318201856586 44-45 19.84732824427481 28.39795429924672 30.407124681933844 21.34759277454463 46-47 21.111693693778804 29.726044436886617 30.26957832036693 18.892683548967646 48-49 19.98387368560301 29.020594122399356 30.765039284268813 20.23049290772882 50-51 18.02792060030078 29.73205357353763 32.30159917482264 19.938426651338943 52-53 19.418789044107342 30.821439988409793 29.39502597560073 20.364744991882134 54-55 18.764201168058374 28.691437650278473 30.719399878123866 21.82496130353929 56-57 20.567806856250474 28.768796755749225 29.410625676935087 21.252770711065214 58-59 21.77841785500677 28.28548493719898 29.719835048950166 20.216262158844085 60-61 19.90475021712042 29.10294654432403 31.437777693866504 19.554525544689046 62-63 19.11020771896457 29.289114002175907 31.327886739396384 20.27279153946314 64-65 19.20921215709928 30.522357150618834 30.10628564049343 20.162145051788464 66-67 19.804105623576625 28.992934698724593 28.945843190000854 22.257116487697935 68-69 18.866162073609676 28.77724133042444 30.719320768388634 21.637275827577245 70-71 21.22291336213754 28.76897462594174 29.14953036963997 20.85858164228075 72-73 20.004630336896383 27.32985480586446 31.738597007920394 20.92691784931876 74-75 18.911916690422455 30.778523088262855 29.465786153814655 20.843774067500036 76-77 19.787177424053233 28.369677664179328 29.643526909081174 22.199618002686265 78-79 19.927834548422798 28.076674218010822 30.91433186347128 21.081159370095104 80-81 20.88616064751475 28.560520304108998 29.46374732944249 21.08957171893376 82-83 19.90694452098472 28.11944481621149 31.116429566764552 20.857181096039238 84-85 19.812183804159734 28.590953393933766 30.248516349676972 21.34834645222953 86-87 19.651629277877845 29.128616593820457 29.97757932259324 21.242174805708455 88-89 20.03590211948477 28.448020659465246 28.964822221522375 22.551254999527604 90-91 20.593872296195972 27.031032229107776 29.844012063277088 22.531083411419168 92-93 21.517105391639603 28.493131699388606 29.913409959272126 20.07635294969966 94-95 20.476836254297968 27.70419582512329 30.05167799928492 21.767289921293827 96-97 19.130264964910733 27.86399601859668 30.900918494626367 22.10482052186622 98-99 20.672764957507262 28.487145080103065 28.65535206919796 22.184737893191713 100-101 20.67671067217575 26.58501713191575 30.075959891161947 22.66231230474655 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3319.0 1 1929.0 2 486.0 3 401.5 4 324.0 5 304.0 6 454.5 7 777.5 8 1029.0 9 1033.0 10 804.5 11 589.5 12 570.5 13 565.0 14 542.5 15 544.0 16 569.5 17 582.5 18 548.0 19 576.5 20 657.5 21 725.0 22 856.0 23 999.0 24 1126.5 25 1399.0 26 1902.0 27 2528.5 28 2973.5 29 3450.0 30 4347.5 31 5253.0 32 6255.0 33 7632.5 34 9053.0 35 10326.0 36 11547.0 37 12737.5 38 12861.0 39 12117.0 40 11538.5 41 10984.0 42 10196.5 43 9799.5 44 9895.5 45 10000.0 46 10963.0 47 16422.5 48 27812.5 49 26824.0 50 14472.0 51 8141.5 52 5682.5 53 4481.0 54 3658.0 55 2999.0 56 2417.5 57 1919.5 58 1604.5 59 1394.0 60 1140.0 61 914.5 62 706.5 63 505.0 64 342.5 65 248.0 66 192.5 67 163.0 68 126.0 69 92.5 70 83.5 71 76.0 72 60.5 73 47.5 74 44.5 75 34.5 76 21.0 77 19.0 78 15.0 79 8.5 80 7.5 81 5.0 82 3.0 83 3.0 84 2.0 85 1.0 86 1.0 87 0.5 88 0.5 89 0.5 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3240282286874663 2 0.09307193802725097 3 0.10090627624503304 4 0.10623362623312484 5 0.11626157915188588 6 0.05891422339772115 7 0.06173458515612269 8 0.06173458515612269 9 0.06455494691452424 10-11 0.06283139250661218 12-13 0.06016771751256628 14-15 0.059070910162076794 16-17 0.06079446456998884 18-19 0.0678453689659927 20-21 0.0689421763164822 22-23 0.08320067187284556 24-25 0.08993820274013813 26-27 0.08445416598769069 28-29 0.08398410569462376 30-31 0.07975356305702144 32-33 0.07191922483923938 34-35 0.32183461398648733 36-37 0.4984205974152951 38-39 0.47256728129661435 40-41 0.45251137545909226 42-43 0.4813417401005302 44-45 0.49011619890444613 46-47 0.48776589743911153 48-49 0.5059415621043659 50-51 0.501554332702408 52-53 0.5009275856449854 54-55 0.4927798738984921 56-57 0.47006029306692404 58-59 0.4506311342868245 60-61 0.41114606966920286 62-63 0.4824385474510197 64-65 0.5059415621043659 66-67 0.5142459606152149 68-69 0.5297579502864234 70-71 0.5256840944131768 72-73 0.5128357797360141 74-75 0.5144026473795705 76-77 0.4908996327262244 78-79 0.47147047394612485 80-81 0.4777379445203505 82-83 0.4874525239104002 84-85 0.4899595121400905 86-87 0.4838487283302205 88-89 0.4945034283064041 90-91 0.5062549356330772 92-93 0.5123657194429472 94-95 0.5206701179537963 96-97 0.5114255988568134 98-99 0.5145593341439262 100-101 0.49262318713413644 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 319108.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.24894049637561 #Duplication Level Percentage of deduplicated Percentage of total 1 78.9330241406301 37.29501760819611 2 11.458643035745597 10.82817485930305 3 3.6843164433941924 5.222401452112512 4 1.6473105881656573 3.113347198371546 5 1.0137752786906356 2.3949903909775223 6 0.6517228440026741 1.847592832584662 7 0.40284403367707333 1.332376764356958 8 0.3088124991360457 1.1672850716972853 9 0.22305536271579554 0.9485216604320494 >10 1.531148950146852 13.450066434342691 >50 0.08893367294599934 2.8724268568455833 >100 0.0358389428289848 3.6002546663148625 >500 0.006636841264626816 2.271286869446786 >1k 0.013273682529253632 11.7844761222988 >5k 6.636841264626817E-4 1.8717812127195725 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5969 1.8705265928776464 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4328 1.3562806322624315 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2801 0.8777592539203029 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2633 0.8251125010968073 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2575 0.806936836431553 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2096 0.6568309161788485 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2043 0.6402221191571505 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2034 0.637401757398749 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1899 0.5950963310227259 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1892 0.5929027163217468 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1778 0.5571781340486607 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1737 0.544329819371498 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1673 0.524273913533976 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1574 0.49324993419155894 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1536 0.4813417401005302 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1348 0.42242751670280904 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1283 0.40205823733657564 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1199 0.37573486092482794 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1102 0.3453376286398335 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1042 0.3265352169171566 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1007 0.31556714341226166 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 934 0.292690875816338 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 878 0.27514195820850623 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 854 0.2676209935194354 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 824 0.25821978765809694 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 743 0.23283653183248307 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 703 0.2203015906840317 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 668 0.20933351717913684 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 564 0.17674267019316345 No Hit GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 544 0.17047519961893778 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 531 0.16640134374569113 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 437 0.13694423204683054 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 432 0.13537736440327414 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 398 0.12472266442709053 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 378 0.11845519385286485 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 363 0.11375459092219561 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 363 0.11375459092219561 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 358 0.1121877232786392 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 352 0.11030748210637152 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 340 0.10654699976183613 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 333 0.10435338506085713 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 6.267470574225654E-4 0.0 18-19 0.0 0.0 0.0 9.401205861338481E-4 0.0 20-21 0.0 0.0 0.0 0.0015668676435564135 0.0 22-23 0.0 0.0 0.0 0.004073855873246675 0.0 24-25 0.0 0.0 0.0 0.007520964689070785 0.0 26-27 0.0 0.0 0.0 0.01833235142961004 0.0 28-29 0.0 0.0 0.0 0.056093861639319606 0.0 30-31 0.0 0.0 0.0 0.1322436291161613 0.0 32-33 0.0 0.0 0.0 0.22500219361470097 0.0 34-35 0.0 0.0 0.0 0.3476879301051682 0.0 36-37 0.0 0.0 0.0 0.5140892738508592 0.0 38-39 0.0 0.0 0.0 0.7209158028003059 0.0 40-41 0.0 0.0 0.0 0.9360467302606015 0.0 42-43 0.0 0.0 0.0 1.141149704802136 0.0 44-45 0.0 0.0 0.0 1.392788648357296 0.0 46-47 0.0 0.0 0.0 1.6812489815360316 0.0 48-49 0.0 0.0 0.0 1.9463629868257768 0.0 50-51 0.0 0.0 0.0 2.2055228950700076 0.0 52-53 0.0 0.0 0.0 2.4521478621657873 0.0 54-55 0.0 0.0 0.0 2.7443686776890583 0.0 56-57 0.0 0.0 0.0 3.1067851636436563 0.0 58-59 0.0 0.0 0.0 3.43739423643406 0.0 60-61 0.0 0.0 0.0 3.806078192962884 0.0 62-63 0.0 0.0 0.0 4.174762149491708 0.0 64-65 0.0 0.0 0.0 4.5661656868521 0.0 66-67 0.0 0.0 0.0 5.034815799039823 0.0 68-69 0.0 0.0 0.0 5.5697444125499835 0.0 70-71 0.0 0.0 0.0 6.128019353949133 0.0 72-73 0.0 0.0 0.0 6.689741404164107 0.0 74-75 0.0 0.0 0.0 7.329023402735125 0.0 76-77 0.0 0.0 0.0 7.954516966042844 0.0 78-79 0.0 0.0 0.0 8.755813078957594 0.0 80-81 0.0 0.0 0.0 9.570270880078219 0.0 82-83 0.0 0.0 0.0 10.346810484224775 0.0 84-85 0.0 0.0 0.0 11.208431001416448 0.0 86-87 0.0 0.0 0.0 12.080236158291237 0.0 88-89 0.0 0.0 0.0 13.016752948844905 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCAATAC 80 2.3092434E-7 41.438538 3 CTAGTCG 55 0.0026148616 34.442425 4 CCTGTAC 80 4.5656023E-4 29.598955 3 GGATATG 65 0.0058648307 29.203148 1 TGTATCG 65 0.0059327376 29.134445 9 GTACCCA 70 0.008421349 27.11721 1 GAGTACT 1130 0.0 25.347614 12-13 GTAAGCG 85 1.10760084E-7 25.178713 94-95 GATATGA 95 0.0012461192 24.925436 2 GTACTTT 1240 0.0 22.908134 14-15 ACTATAT 125 2.3018091E-4 22.724869 8 CTTTGCG 345 0.0 21.963284 2 CTCGTCA 65 2.914602E-4 21.950674 76-77 CGTCATC 55 0.0028550448 21.618088 78-79 GTATTAG 110 0.002888643 21.570509 1 ATGGGAT 245 7.728886E-9 21.262924 5 GTGTAGC 135 3.8118768E-4 21.091164 1 GTATAGG 115 0.0037378608 20.63266 1 TGGAGTG 115 0.0037823494 20.590578 5 TTGCGTT 405 0.0 19.878807 4 >>END_MODULE